WO2010045753A1

WO2010045753A1 - Flushtype culture tank

Info

Publication number: WO2010045753A1
Application number: PCT/CN2008/001782
Authority: WO
Inventors: 黄玲惠; 陈有利
Original assignee: Huang Lynn L H; Chen Yu Lea
Priority date: 2008-10-22
Filing date: 2008-10-22
Publication date: 2010-04-29

Abstract

A flushtype culture tank comprises a support (10), a hollow body (20) and a membrane (30). The support includes an annular bell socket (11) and several arms (12) disposed around the bottom of the annular bell socket. A body-height-adjustment space (14) is provided inside the annular bell socket and a first adjustable part (13) is set on the sidewall of the body-height-adjustment space. A second adjustable part (21) which corresponds to the first adjustable part is provided on the periphery of the hollow body. The hollow body is movably fixed in the body-height-adjustment space of the annular bell socket and can be lifted or dropped through cooperation of the first and second adjustable part. The membrane is fixed on the bottom of hollow body and is tightened and flattened. The flushtype culture tank has many advantages, such as simple structure, easy fabrication, low cost and so on.

Description

Embedded culture tank

The present invention relates to a cell culture apparatus, and more particularly to a cell culture apparatus which can be applied to the field of living cell culture, or as a carrier used in the field of microscopic observation of biological materials, so as to facilitate microscopy of living cells or other biological materials. Observe and record the embedded culture tank. Background technique

In the field of research and application of cell biology, for in vitro cell culture, or for tissue formation, cell line establishment, cell physiology and toxicity testing, cell migration observation, cells and another cell or other extracellular matrix or factor Various research and applications, or when living experiments are difficult, usually require an embedded cell culture tank to provide stem cells and other types of cells for in vitro culture.

The current cell culture tank design, as shown in FIG. 7 , mainly includes a tank body 40 and a diaphragm 50 , wherein the tank body 40 forms an upper and lower through-hole accommodating space, and the tank body The bottom of the 40 is integrally formed with three support legs 41, and the diaphragm 50 is disposed at the bottom of the groove body 40.

In the foregoing cell culture tank design, the tank body is formed by casting by casting, and the prepared cell culture tank is a fixed shape and immutable shape, and has no adjustability, and is expensive due to the mold. If you need to order a product other than the specifications, you usually have to pay a very high fee.

In other words, the cell culture tank is designed as a tripod structure, and the fluidity of the culture medium and the convenience of observation and observation are mutually inhibited, because if the height of the culture tank is low, the cell culture is performed. When the tank is immersed in the liquid medium, the membrane height is too low, and the culture solution and the additive are equal to the permeability inside and outside the membrane, which is not conducive to cell culture.

If the culture trough height is designed to be biased, although there is no problem of poor permeability of the above medium, when the user wants to observe live cells or image, such as high magnification microscopic image observation. When a high magnification objective lens is used, the high magnification lens has a large NA value and a short working distance. At this time, the growth chamber of the culture tank is too high, and the cell culture tank does not have The lifting adjustment function makes it difficult to adjust the focal length, so that the acquired image is not ideal. Summary of the invention

The main object of the present invention is to overcome the above disadvantages of the prior art, and to provide an embedded culture tank comprising a tripod, a hollow body and a diaphragm, wherein the diaphragm is fixed at the bottom end of the body. The body is disposed on the socket of the tripod, and the first and second adjustment portions are designed to cooperate with each other, so that the body can be lifted and adjusted in the tripod. When the embedded culture tank is in use, The height of the body provided with the membrane can be adjusted according to the needs of culturing cells or microscopic observation, and the convenience of adjusting the focal length of the microscope can be facilitated to facilitate image acquisition, and the composition of the culture tank is simple and easy to manufacture. The advantage of low cost can overcome the shortcomings of the existing cell culture tank without the lifting adjustment function.

The object of the present invention is achieved by the following technical solutions.

The embedded culture tank of the present invention is characterized in that it comprises:

a tripod includes an annular sleeve portion and a plurality of support legs, wherein the support legs are distributed on a bottom surface of the socket portion, and a lifting space is formed in the socket portion, and is disposed on the side wall of the lifting space First adjustment unit;

a hollow body, a second adjusting portion corresponding to the first adjusting portion is disposed on the outer peripheral surface of the body, so that the body is movably mounted in the lifting space of the tripod socket portion, and passes through the first and the first The combination of the two adjustment parts can be adjusted up and down to adjust the positioning;

a diaphragm which is fixed to the bottom end of the body and has a tight and flat shape.

In the above-mentioned embedded culture tank, the first adjustment portion disposed on the inner side wall of the socket portion is formed with a convex granule; the second adjustment portion disposed on the outer peripheral surface of the body is a concave guide groove, and the guide groove has A longitudinal groove segment and a transverse groove segment engaging the bottom end of the longitudinal groove segment form an L-shaped groove into which the convex first adjustment portion projects.

In the above-mentioned embedded culture tank, the first adjusting portion of the foot frame disposed on the inner side wall of the socket portion is formed with an internal thread; the second adjusting portion disposed on the outer peripheral surface of the body is formed with a corresponding external thread, and the body is screwed to In the socket of the tripod.

The beneficial effects of the embedded culture tank of the present invention include: a tripod comprising an annular sleeve portion and a plurality of support legs, wherein the support legs are distributed on the bottom surface of the sleeve portion, and a sleeve portion is formed in the sleeve portion a lifting space, and a first adjusting portion is disposed on the side wall of the lifting space; a hollow body having a second adjusting portion corresponding to the first adjusting portion on the outer peripheral surface of the body, so that the body is movably mounted on the body The lifting space of the tripod socket can be adjusted up and down by the combination of the first and second adjusting portions; and a diaphragm fixed to the bottom end of the body is tight and flat. It includes at least: 1. It has a lifting adjustment positioning function. The embedded culture tank designed by the invention adopts a combination of the first and second adjustment portions between the main body and the tripod, so that the body provided with the diaphragm on the bottom surface can be lifted and lowered in the tripod. 2, easy to manufacture, low cost. The foot frame, the body and the like used in the embedded culture tank designed by the invention are simple in shape and can be manufactured by using a plastic material such as a ruthenium molecular polymer by a general machining tool by turning, ..., etc. There is no need to order special molding molds, which are easy to manufacture and low in cost. 3. The specifications have good variability and are easy to customize. The main body design of the embedded culture tank designed by the invention can be formed by using a general mechanical processing machine mechanism, so that the size of the main body of the product can be changed according to the customer's use requirements, and can be realized, and the basic manufacturing demand of the product is required. It can be large or small, and can be used to obtain the required products without having to pay a large amount of money, and has good production economy. 4. The culture solution and the like have good permeability. The culture tank designed by the invention can be used for cell culture, and the body with the membrane on the bottom surface can be elevated by means of a tripod, so that the culture medium and the additives, etc. can obtain good communication at the membrane. Permeability. 5, the microscope to observe the concern that the focal length is difficult to adjust. The main body of the embedded culture tank designed by the invention (including the body and the tripod, etc.) has an adjustable function of lifting and lowering, so that the user wants to perform microscopic observation of the cells in the middle or completion of the culture medium in the culture tank. The embedded culture tank can be placed under a microscope, and in addition to focusing by the adjustment mechanism of the microscope itself, the mechanism of the culture tank itself can be adjusted up and down, so that the diaphragm disposed at the bottom end of the body can be accessed. The objective lens of the microscope is focused by an auxiliary microscope, so that the user can observe the growth of the cells in the culture tank or capture images by operating the microscope.

Therefore, it is possible to solve the disadvantage that the existing cell culture tank does not have a lifting adjustment function. BRIEF DESCRIPTION OF THE DRAWINGS:

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a perspective exploded view showing the structure of a first preferred embodiment of the embedded culture tank of the present invention.

2 is a perspective view showing the height of the lifting and adjusting body of the first preferred embodiment of the embedded culture tank shown in FIG.

3 is another perspective view of the height of the lifting and adjusting body of the first preferred embodiment of the embedded culture tank shown in FIG. 1. 4 is a cross-sectional view showing the first preferred embodiment of the embedded culture tank shown in FIG. 1 with the height of the body lowered.

Fig. 5 is a perspective exploded view showing the structure of a second preferred embodiment of the embedded culture tank of the present invention.

Figure 6 is a cross-sectional view showing the second preferred embodiment of the embedded culture tank shown in Figure 5.

Fig. 7 is a schematic cross-sectional view showing the structure of a conventional embedded cell culture tank. The main symbols in the figure are: 10 tripod, 11 socket, 12 support legs, 13 first adjustment part, 14 lifting space, 20 body, 21 second adjustment part, 211 longitudinal groove section, 212 transverse groove section, 30 diaphragms, 40 slots #, 41 support feet, 50 diaphragms. detailed description

Referring to FIG. 1 and FIG. 5, it is a plurality of concrete and feasible preferred embodiments for embedding the culture tank of the present invention. As can be seen from the figure, the embedded culture tank includes a tripod 10, a body 20, and a diaphragm 30; wherein:

The tripod 10 includes an annular sleeve portion 11 and a plurality of support legs 12. The support legs 12 are distributed downwardly on the bottom surface of the socket portion 11. A lifting and lowering space 14 is formed in the socket portion 11. And a first adjustment portion 13 located on a side wall of the lifting and lowering activity space 14.

The body 20 can be a hollow tubular body, and can be assembled and lowered in the socket portion 11 of the stand 10, and a second adjustment corresponding to the first adjusting portion 13 is disposed on the outer peripheral surface of the body 20. The portion 21 allows the main body 20 to be lifted and lowered in the stand 10 by the corresponding cooperation of the first adjusting portion 13 and the second adjusting portion 21.

The diaphragm 30 can be a film made of a material such as porous nylon, and has a film thickness of about ι μηη to 60 um, and a hole size is used as needed. The diaphragm 30 can be thermocompression-bonded. Or other fixing means is fixed on the bottom end of the body 20, and the diaphragm 30 is disposed at the bottom end of the body 20 in a tight and flat state for providing the growth of living cells to the surface thereof, and for microscopic observation.

As shown in FIG. 1 , the first adjusting portion 13 of the stand 10 disposed on the inner side wall of the socket portion 11 is a convex design; and the second adjusting portion is disposed on the outer circumferential surface of the body 20 . 21 can be designed as a concave guide groove, the guide groove has a longitudinal groove segment 211 and a transverse groove segment 212 that is connected to the bottom end of the longitudinal groove segment 211 to form an L-shaped groove, and the convex groove is provided. The first adjusting portion 13 moves along the channel-shaped second adjusting portion 21, so that the body 20 can be lifted and adjusted in the stand 10, as shown in FIG. 2 to FIG.

As shown in FIG. 5 and FIG. 6 , the first adjusting portion 13 of the tripod 10 disposed on the inner side wall of the socket portion 11 is designed as an internal thread; and is disposed on the outer circumferential surface of the body 20 . The second adjusting portion 21 is designed to correspond to the external thread of the internal thread, so that the body 20 is screwed into the socket portion 11 of the stand 10, and the body 20 can be lifted and lowered in the stand 10 by rotation. Adjust the positioning.

The invention adopts the above-mentioned embedded culture tank design with lifting adjustment positioning function, and the culture tank can be dipped in use. The medium containing the culture solution, the additive, and the like is placed, and the living cells to be cultured are placed on the surface of the porous membrane having a suitable permeability and tightness, and the cells are allowed to survive in the culture medium. During the culture, the user can extend the body of the culture tank on the tripod to a higher position, so that the culture liquid and the additive have permeability in the membrane, so that the cells on the surface of the membrane can be well cultured. When the cell culture is in the middle or completed, if you want to observe through the microscope, the body in the culture tank can be lowered, so that the diaphragm can be closer to the objective lens of the microscope, so as to adjust the focal length of the microscope, and the obtained image is ideal.

In summary, the design of the embedded culture tank with the lifting adjustment function can overcome the problem that the existing embedded cell culture tank adjusts the focal length when viewed under the microscope, and is simple and easy to manufacture. The structural design makes it easy to operate and reduce the manufacturing cost. Therefore, the present invention is an industrially valuable invention in the technical field of cell culture technology or microscopic observation of other biological materials.

The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way. Any simple modifications, equivalent changes and modifications made to the above embodiments in accordance with the technical spirit of the present invention are still Within the scope of the technical solution of the present invention.

Claims

Claim

1. An embedded culture tank, comprising:

a hollow body, a second adjusting portion corresponding to the first adjusting portion is disposed on the outer peripheral surface of the body, so that the body is movably mounted in the lifting space of the tripod socket portion, and passes through the first and the first The combination of the two adjustment parts can be adjusted up and down;

The embedded culture tank according to claim 1, wherein the first adjusting portion provided on the inner side wall of the socket portion is formed with a convex granule; and the second adjusting portion disposed on the outer peripheral surface of the body is formed. a concave guiding groove, the guiding groove has a longitudinal groove segment and a transverse groove segment connected to the bottom end of the longitudinal groove segment to form an L-shaped groove, wherein the convex first adjusting portion projects into the groove .

The embedded culture tank according to claim 1, wherein the first adjusting portion of the foot frame disposed on the inner side wall of the socket portion is formed with an internal thread; and the second adjusting portion disposed on the outer peripheral surface of the body is A corresponding external thread is formed, and the body is screwed into the socket of the tripod.