CN1896222A - Microbe culturing dish made from adjusting gas - Google Patents

Microbe culturing dish made from adjusting gas Download PDF

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Publication number
CN1896222A
CN1896222A CN 200610042920 CN200610042920A CN1896222A CN 1896222 A CN1896222 A CN 1896222A CN 200610042920 CN200610042920 CN 200610042920 CN 200610042920 A CN200610042920 A CN 200610042920A CN 1896222 A CN1896222 A CN 1896222A
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CN
China
Prior art keywords
wire loop
cultivation device
top cover
microbial cultivation
base
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN 200610042920
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Chinese (zh)
Inventor
蒲鹏
梁宗锁
王妮妮
兰晓继
杜淑媛
陈越
杨学贞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Northwest A&F University
Original Assignee
Northwest A&F University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Northwest A&F University filed Critical Northwest A&F University
Priority to CN 200610042920 priority Critical patent/CN1896222A/en
Publication of CN1896222A publication Critical patent/CN1896222A/en
Pending legal-status Critical Current

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Abstract

A microbiological culturing device made from adjustable gas consists of culture dish and case containing it, the case is constituted of end cap and base, a observation window and at least one reagent-feeding channel are arranged at case top, at least one reactive chamber and one indicating chamber are set in the case, both enclose a circular pool, and internal arm and base of circular pool constitute an area for containing culture dish. It is cheap, simple and practical.

Description

The microbial cultivation device that a kind of adjustable gas is formed
Technical field
The invention belongs to the microorganism culturing technical field, be specifically related to the microbial cultivation device that a kind of adjustable gas is formed.
Technical background
We generally adopt anaerobic jar, moisture eliminator or back-off Plating at present anaerobion solid separation and Culture.The shortcoming of these method maximums is the microorganism growth situations that can not observe at any time in each plate, uses trouble in addition.Anaerobic jar is relatively more expensive; The space of wasting in the moisture eliminator is big, waste oxygen uptake reagent; Back-off plate anaerobism is not strict, pollutes easily yet.
Summary of the invention
At the shortcoming and defect of above-mentioned prior art, the object of the present invention is to provide a kind of adjustable gas to form, can provide strict anaerobic condition, can observe the microorganism growth situation at any time, can reflect air chamber anaerobic degree, save the microbial cultivation device of oxygen uptake reagent.
Realize that foregoing invention purpose technical scheme is the microbial cultivation device that a kind of adjustable gas is formed, comprise a culture dish, it is characterized in that, this microbial cultivation device is to be rotatably connected by a top cover and base by thread to be one, be equipped with seal washer and ring groove respectively on the last inner edge of its base, top cover is provided with rectangular strip, the upper end of rectangular strip is equipped with wire loop, wire loop is provided with pin hole, and the wire loop upper surface is equipped with gasket, and another wire loop is connected by screw thread rotation with top cover, on the step of this wire loop gasket is housed, be inlaid with the perspective window on the gasket, the corresponding place of top cover and pin hole is provided with pin hole, and the reaction chamber of base is built-in with culture dish.
Described ring groove is divided at least two independently unit.
Description of drawings
Fig. 1 is the sectional view of microbial cultivation device of the present invention.
Fig. 2 is the front view of microbial cultivation device top cover 1 of the present invention.
Fig. 3 is the vertical view of microbial cultivation device top cover 1 of the present invention.
Fig. 4 is the front view of microbial cultivation device wire loop 2 of the present invention.
Fig. 5 is the vertical view of microbial cultivation device wire loop 2 of the present invention.
Fig. 6 is the front view of microbial cultivation device wire loop 3 of the present invention.
Fig. 7 is the vertical view of microbial cultivation device wire loop 3 of the present invention.
Fig. 8 is the front view of microbial cultivation device base 4 of the present invention.
Fig. 9 is the vertical view of microbial cultivation device base 4 of the present invention.
Figure 10 is the front view of microbial cultivation device ring groove 5 of the present invention.
Figure 11 is the vertical view of microbial cultivation device ring groove 5 of the present invention.
Figure 12 is the front view of microbial cultivation device perspective window 6 of the present invention.
Figure 13 is the vertical view of microbial cultivation device perspective window 6 of the present invention.
Figure 14 is the front view of microbial cultivation device gasket 7 of the present invention.
Figure 15 is the vertical view of microbial cultivation device gasket 7 of the present invention.
Figure 16 is the drawing in side sectional elevation of microbial cultivation device gasket 8 of the present invention.
Figure 17 is the longitudinal diagram of microbial cultivation device gasket 8 of the present invention
Figure 18 is the mansion view of microbial cultivation device gasket 9 of the present invention.
The embodiment that provides below in conjunction with accompanying drawing and contriver is to this bright being described in further detail.
Embodiment
Figure 1 shows that the sectional view of microbial cultivation device of the present invention, Fig. 2 to Figure 15 is the front view and the vertical view of top cover 1, wire loop 2, wire loop 3, base 4, ring groove 5, perspective window 6, gasket 7 successively, Figure 16, Figure 17 divide horizontal stroke, the longitudinal sectional drawing of other gasket 8, and Figure 18 is the mansion view of gasket 9.
The microbial cultivation device that a kind of adjustable gas is formed, comprise a culture dish 14, it is characterized in that, this microbial cultivation device is to be connected as one by screw thread rotation by a top cover 1 and base 4, be equipped with seal washer 9 and ring groove 5 on the last inner edge of its base 4 respectively, top cover 1 is provided with rectangular strip 12, the upper end of rectangular strip 12 is equipped with wire loop 3, wire loop 3 is provided with pin hole 11, wire loop 3 upper surfaces are equipped with gasket 8 respectively, wire loop 2 and top cover 1 are connected by screw thread rotation, gasket 7 is housed on the step of wire loop 2, be inlaid with perspective window 6 on the gasket 7, top cover 1 is provided with pin hole 10 with pin hole 11 corresponding places, and the reaction chamber of base 4 is built-in with culture dish 14.
Described ring groove 5 is divided at least two independently unit.
Basic functional principle:
Injection orifice 10 by top cover 1 upper surface adds reagent A, perhaps bleeds with vacuum pump, injects the gas of the ratio that proportionally prepares then, with the reagent B reaction in the ring groove 5, begin the oxygen of absorption unit inside immediately, cause low-oxygen environment, for the anerobe growth; Whether the inner oxygen of device is absorbed, and can observe by the colour-change of indicator, and originally indicator is blue, when indicator is colourless, illustrates that indoor oxygen is absorbed, and can reach the cultivation requirement; When indicator becomes blueness, illustrate that device inside has entered oxygen, can not satisfy culture condition, need to change the reagent in ring groove 5 or the ring groove 5.
Concrete using method:
1. prepare certain density reagent A earlier.
2. before using, take by weighing pack into the annular reaction chamber of ring groove 5 of a certain amount of solid reagent B earlier.
3. there is inoculation the culture dish 14 of microorganism to put into the middle part of base 4.Ring groove 5 is enclosed within the outside of plate.
4. top cover 1 is screwed on the base 4.
5. draw the circular hole 10 injection ring grooves 5 of a certain amount of reagent A with plastic injector from top cover 1.
6. device is placed common incubator to cultivate.
When 7. opening, by rotation, top cover 1 separates with base 4, takes out culture dish, carries out form and the biochemical property test of microorganism.
When 8. needing continue to cultivate, change ring groove 5 or change reagent B in the ring groove 5, repeat 4.~6. step.
Mentioned reagent A, B change according to using device of the present invention to cultivate different microorganisms.
Precaution:
1. this device in use keeps flat as far as possible, the too much even inversion of not tiltable.
2. each opening unit needs to change a reagent B, not reproducible utilization.Black water-absorbing material after will using is buried with breadboard waste liquid.
3. use liquid A to have certain corrodibility,, use light NaHCO again if a large amount of flushing with clean water are used in contact accidentally immediately 3Flushing, the severe patient treatment of seeking medical advice.
4. reagent B in use avoids long-time strong illumination as far as possible.
The present invention compares with current existing equipment, and its outstanding feature is:
1. can observe microbial growth situation in each device culture dish at any time.
If 2. operation failure does not cause oxygen-free environment, can reflect by the indicator in the ring groove 5, can in time be corrected.
3. strict oxygen free condition can be provided.
4. at the bottom of simple to operate, practical, the cost more in promoting the use of.
5. can regulate the gas composition in the culturing process within the specific limits.
6. each plate works alone, and has avoided misoperation to cause all to cultivate the accident of failure.

Claims (2)

1. the microbial cultivation device formed of an adjustable gas, comprise a culture dish (14), it is characterized in that this microbial cultivation device is to be connected as one by screw thread rotation by a top cover (1) and base (4), be equipped with seal washer (9) and ring groove (5) on the last inner edge of its base (4) respectively, top cover (1) is provided with rectangular strip (12), the upper end of rectangular strip (12) is equipped with wire loop (3), wire loop (3) is provided with pin hole (11), wire loop (3) upper surface is equipped with gasket (8) respectively, wire loop (2) is connected by screw thread rotation with top cover (1), gasket (7) is housed on the step of wire loop (2), be inlaid with perspective window (6) on the gasket (7), top cover (1) is provided with pin hole (10) with the corresponding place of pin hole (11), and the reaction chamber of base (4) is built-in with culture dish (14).
2. the microbial cultivation device that adjustable gas according to claim 1 is formed is characterized in that described ring groove (5) is divided at least two independently unit.
CN 200610042920 2006-06-02 2006-06-02 Microbe culturing dish made from adjusting gas Pending CN1896222A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200610042920 CN1896222A (en) 2006-06-02 2006-06-02 Microbe culturing dish made from adjusting gas

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 200610042920 CN1896222A (en) 2006-06-02 2006-06-02 Microbe culturing dish made from adjusting gas

Publications (1)

Publication Number Publication Date
CN1896222A true CN1896222A (en) 2007-01-17

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN 200610042920 Pending CN1896222A (en) 2006-06-02 2006-06-02 Microbe culturing dish made from adjusting gas

Country Status (1)

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CN (1) CN1896222A (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010045753A1 (en) * 2008-10-22 2010-04-29 Huang Lynn L H Flushtype culture tank
CN101624567B (en) * 2009-08-13 2012-02-15 浙江大学 Microorganism online observation device
CN104673628A (en) * 2015-02-16 2015-06-03 陈子江 Heat-preservation culturing device with gas control function
CN106434323A (en) * 2016-12-05 2017-02-22 无锡艾科瑞思产品设计与研究有限公司 Microorganism culture and detection device capable of controlling oxygen environments
CN110241000A (en) * 2019-05-15 2019-09-17 铜仁学院 A kind of microbial bacteria culture apparatus

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010045753A1 (en) * 2008-10-22 2010-04-29 Huang Lynn L H Flushtype culture tank
CN101624567B (en) * 2009-08-13 2012-02-15 浙江大学 Microorganism online observation device
CN104673628A (en) * 2015-02-16 2015-06-03 陈子江 Heat-preservation culturing device with gas control function
CN106434323A (en) * 2016-12-05 2017-02-22 无锡艾科瑞思产品设计与研究有限公司 Microorganism culture and detection device capable of controlling oxygen environments
CN110241000A (en) * 2019-05-15 2019-09-17 铜仁学院 A kind of microbial bacteria culture apparatus
CN110241000B (en) * 2019-05-15 2022-05-31 铜仁学院 Microbial cultivation device

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Open date: 20070117