CN110241000B - Microbial cultivation device - Google Patents
Microbial cultivation device Download PDFInfo
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- CN110241000B CN110241000B CN201910402660.5A CN201910402660A CN110241000B CN 110241000 B CN110241000 B CN 110241000B CN 201910402660 A CN201910402660 A CN 201910402660A CN 110241000 B CN110241000 B CN 110241000B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a microbial bacteria culture device, which comprises a shell, wherein a fixed plate is arranged on the left upper side of the shell, a PLC (programmable logic controller) is arranged on the front side surface of the fixed plate, a temperature control purification system is arranged on the left side surface of the shell, a negative pressure pipe is arranged on a left side surface interface of the shell, a vacuum pump is arranged at a left end inlet of the negative pressure pipe, a first electromagnetic valve is arranged in the middle of the negative pressure pipe, an electric telescopic rod in the horizontal direction is fixed on the left side surface of an inner cavity of the shell, a horizontal mounting frame is fixed on a right telescopic arm of the electric telescopic rod, the microbial bacteria culture device can be used for placing culture and inoculation of microbial bacteria in the shell for operation, has good sealing performance, can be used for controlling the temperature and cleanliness of a bacterial colony environment through the temperature control purification system, is used for controlling air pressure through the vacuum pump, and is used for inoculating microbial bacteria through the matching of a culture pipe, a seed receiving pipe and a flushing pipe, the culture environment of the microorganism bacteria is ensured to be controllable all the time, and the culture quality is ensured.
Description
Technical Field
The invention relates to the technical field of microbial culture, in particular to a microbial culture device.
Background
Present microbial flora is closely related with human life, consequently need use the incubator device when studying it, in microbial pesticide's production, need cultivate biological bacterial in a large number, after inoculating the bacterin with the culture medium, through the stirring, and provide appropriate temperature and oxygen, the bacterial constantly breeds, but prior art's operation is all through manual realization, complex operation, the error appears easily, the environment of bacterial colony changes during the operation, liquid medium to the inconvenient control of inoculation of solid medium, need more experience, environmental control is complicated simultaneously, the cost of cultivateing has been improved.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the existing defects, and provide a microbial bacteria culture device which is simple in structure, convenient to use, capable of effectively controlling the environment of bacterial colonies, performing closed operation, improving the culture stability, automatically performing inoculation, convenient to operate, improving the quality of microbial bacteria culture and effectively solving the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: a microbial bacteria culture device comprises a shell, wherein a fixed plate is arranged on the upper left side of the shell, a PLC (programmable logic controller) is installed on the front side face of the fixed plate, a temperature control purification system is arranged on the left side face of the shell, a negative pressure pipe is arranged on a left side face interface of the shell, a vacuum pump is arranged at the left end inlet of the negative pressure pipe, a first electromagnetic valve is arranged in the middle of the negative pressure pipe, a horizontal electric telescopic rod is fixed on the left side face of an inner cavity of the shell, a horizontal mounting rack is fixed on a right telescopic arm of the electric telescopic rod, rollers are arranged on the lower surface of the mounting rack, a turntable is arranged in a circular through groove at the right end of the mounting rack, a chuck is fixed on the lower end face of the turntable, a first motor is arranged at the right end of the bottom face of the inner cavity of the shell, a square shaft matched with the chuck is fixed on an output shaft on the upper side of the first motor, and a side door is arranged on the right side face of the shell, the side door comprises a side door body, a side door cover, a cover plate, an electric push rod, a placing dish, a glass cover, a culture tube and a connecting seat, wherein the side door body is hinged with the lower side edge position of the side door body through a hinge, the upper end of the cover plate is connected with the side surface of a shell through a hasp, the inner cavity right side surface of the shell is provided with the temperature and humidity sensor and the air pressure sensor, the inner cavity upper surface of the shell is provided with a lamp, the upper surface outer side of the shell is fixedly provided with the electric push rod in the vertical direction, the upper end telescopic arm of the electric push rod is fixedly provided with the placing dish, the upper side opening of the placing dish is provided with the glass cover, the bottom interface of the placing dish is provided with the culture tube in the vertical direction, the culture tube penetrates through a preformed hole corresponding to the upper surface of the shell, the middle of the culture tube is provided with the second electromagnetic valve and the first negative pressure pump, the lower end side surface of the culture tube is fixedly provided with the L-shaped connecting seat, the improved washing machine is characterized in that a flushing pipe is arranged in a shaft hole of the connecting seat, two positioning rings I which are respectively attached to the upper end face and the lower end face of the connecting seat are fixed at the lower end of the flushing pipe, a reserved hole at the position corresponding to the shell is penetrated through the upper end of the flushing pipe, stirring pieces are radially distributed on the side face of the lower end of the flushing pipe, the input end of the PLC is respectively and electrically connected with the output ends of an external power supply, a temperature and humidity sensor and an air pressure sensor, and the output end of the PLC is respectively and electrically connected with the input ends of a vacuum pump, an electric telescopic rod, a first motor, a lamp, an electric push rod, a first electromagnetic valve, a second electromagnetic valve, a first negative pressure pump, a second negative pressure pump and a third electromagnetic valve.
As a preferable technical scheme of the invention, the temperature control purification system comprises a circulating pipe, two ends of the circulating pipe are respectively connected with two interfaces on the upper side and the lower side of the left side surface of the shell, a circulating pump and a heater are arranged in the middle of the circulating pipe, and input ends of the circulating pump and the heater are electrically connected with an output end of the PLC.
As a preferable technical scheme of the invention, the right end of the circulating pipe is provided with a heat exchanger, the left side of the shell is provided with a fan corresponding to the heat exchanger, and the input end of the fan is electrically connected with the output end of the PLC.
As a preferable technical scheme of the invention, an electromagnetic three-way valve is arranged in the middle of the circulating pipe and is positioned on the upper side of the circulating pump, a side interface of the electromagnetic three-way valve is connected with an air outlet of the atomizer, a water injection pipe is arranged on the upper surface of the atomizer, and input ends of the electromagnetic three-way valve and the atomizer are respectively and electrically connected with an output end of the PLC.
As a preferable technical scheme of the invention, a second positioning ring is fixed on the side surface of the rotary table and is positioned on the upper side of the mounting frame, and a clamping seat is oppositely arranged on the upper surface of the rotary table.
As a preferred technical scheme of the invention, the chuck and the turntable are coaxial, and the axis of the chuck is provided with a square hole matched with the square shaft.
As a preferable technical scheme of the invention, the circumferential surface of the chuck is provided with a notch, and the inner side end of the notch extends to one side of the square hole and is communicated with the square hole.
As a preferable technical scheme of the invention, the outer side surface of the cover plate is provided with a handle, and the inner side surface of the cover plate is provided with a rubber mat.
As a preferable technical scheme of the present invention, the right side surface of the housing is transitionally connected with an extension cover, the inner side of the upper surface of the extension cover is provided with a negative ion generator, and the input end of the negative ion generator is electrically connected with the output end of the PLC controller.
As a preferable technical scheme of the present invention, a second motor is provided on an upper surface of the housing, a vertical tooth post is fixed on an output shaft of the second motor, the tooth post is engaged with a gear sleeve at an upper end of the flushing pipe, and an input end of the second motor is electrically connected with an output end of the PLC controller.
Compared with the prior art, the invention has the beneficial effects that:
1) according to the microbial bacterium culture device, the culture and inoculation of the microbial bacterium are both arranged in the shell for operation, the device has good sealing performance, the temperature and the cleanliness of a bacterial colony environment can be controlled through the temperature control purification system, the air pressure is controlled through the vacuum pump, the inoculation of the microbial bacterium is carried out through the matching of the culture pipe, the inoculation pipe and the flushing pipe, the culture environment of the microbial bacterium is guaranteed to be always controllable, and the culture quality is guaranteed;
2) the microbial strain culture device circularly controls gas in an inner cavity of a shell through a circulating pipe of a temperature control purification system, can heat the circulated gas through a heater, can cool environmental gas through the cooperation of a heat exchanger and a fan, and controls environmental pressure through a vacuum pump so that bacterial colonies are in an environment with proper temperature and pressure;
3) according to the microbial strain culture device, the translational mounting frame and the rotary table are arranged at the bottom of the inner cavity of the shell, the liquid culture medium and the solid culture medium can be fixed on the clamping seat, so that inoculation operation is facilitated, the rotary table and the chuck can be pulled to the outer side of the shell, so that the culture medium is convenient to fix, the negative ion generator can interfere with the external operation environment, dust can be suppressed, and dust is prevented from entering the culture medium when the culture medium is fixed;
4) this microbial bacteria culture device carries out the coating of microbial bacteria through the rotatable holder of culture tube cooperation of liftable, can carry out the inoculation transfer between liquid medium and the solid medium through the inoculation pipe, can wash the microbial bacteria of solid medium through the flushing pipe, makes things convenient for the absorption of inoculation pipe to shift, and the flushing pipe can drive the stirring piece and rotate to make the bacterial colony distribute evenly.
Drawings
FIG. 1 is a schematic view of the overall cross-sectional structure of the present invention;
FIG. 2 is an enlarged view of the structure at A of the present invention;
FIG. 3 is a schematic view of the chuck structure of the present invention.
In the figure: 1 shell, 2 PLC controllers, 3 temperature control purification systems, 301 circulating pipes, 302 circulating pumps, 303 heaters, 304 heat exchangers, 305 fans, 306 electromagnetic three-way valves, 307 atomizers, 308 water injection pipes, 4 negative pressure pipes, 401 vacuum pumps, 402 first electromagnetic valves, 5 electric telescopic rods, 6 mounting racks, 601 rollers, 7 turntables, 701 clamping seats, 702 positioning ring two, 8 clamping discs, 801 square holes, 802 missing grooves, 9 first motors, 10 square shafts, 11 side doors, 12 cover plates, 13 handles, 14 rubber mats, 15 buckles, 16 extending covers, 17 negative ion generators, 18 temperature and humidity sensors, 19 air pressure sensors, 20 lamps, 21 electric push rods, 22 placing dishes, 23 glass covers, 24 culture pipes, 25 second electromagnetic valves, 26 first negative pressure pumps, 27 seed pipes, 28 second negative pressure pumps, 29 third electromagnetic valves, 30 connecting seats, 31 flushing pipes, 32 gear sleeves, 33 second motors, 34 toothed columns, 30 connecting seats, 31 flushing pipes, 32 gear sleeves, and the like, 35 stirring sheet, 36 positioning ring one.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-3, the present invention provides a technical solution: a microbial cultivation device comprises a shell 1, a fixing plate is arranged on the upper left side of the shell 1, a PLC (programmable logic controller) 2 is installed on the front side face of the fixing plate, a temperature control purification system 3 is arranged on the left side face of the shell 1, the temperature control purification system 3 comprises a circulating pipe 301, two ends of the circulating pipe 301 are respectively connected with two interfaces on the upper side and the lower side of the left side face of the shell 1, a circulating pump 302 and a heater 303 are arranged in the middle of the circulating pipe 301, input ends of the circulating pump 302 and the heater 303 are electrically connected with an output end of the PLC 2, a heat exchanger 304 is arranged at the right end of the circulating pipe 301, a fan 305 is arranged at the position, corresponding to the heat exchanger 304, on the left side face of the shell 1, an input end of the fan 305 is electrically connected with an output end of the PLC 2, an electromagnetic three-way valve 306 is arranged in the middle of the circulating pipe 301, the electromagnetic three-way valve 306 is positioned on the upper side of the circulating pump 302, a side interface on the electromagnetic three-way valve 306 is connected with an air outlet of an atomizer 307, the upper surface of the atomizer 307 is provided with a water injection pipe 308, the input ends of the electromagnetic three-way valve 306 and the atomizer 307 are respectively and electrically connected with the output end of the PLC controller 2, the left side interface of the shell 1 is provided with a negative pressure pipe 4, the left end inlet of the negative pressure pipe 4 is provided with a vacuum pump 401, the middle part of the negative pressure pipe 4 is provided with a first electromagnetic valve 402, the left side surface of the inner cavity of the shell 1 is fixed with an electric telescopic rod 5 in the horizontal direction, the right telescopic arm of the electric telescopic rod 5 is fixed with a horizontal mounting rack 6, the lower surface of the mounting rack 6 is provided with a roller 601, the circular through groove at the right end of the mounting rack 6 is internally provided with a turntable 7, the side surface of the turntable 7 is fixed with a second positioning ring 702, the second positioning ring 702 is positioned at the upper side of the mounting rack 6, the upper surface of the turntable 7 is relatively provided with a clamping seat 701, the lower end surface of the turntable 7 is fixed with a chuck 8, the right end of the bottom surface of the inner cavity of the shell 1 is provided with a first motor 9, the output shaft 10 matched with the chuck 8 is fixed on the upper side of the first motor 9, the chuck 8 and the turntable 7 are coaxial, the axis of the chuck 8 is provided with a square hole 801 matched with the square shaft 10, the circumferential surface of the chuck 8 is provided with a notch 802, the inner side end of the notch 802 extends to one side of the square hole 801 and is communicated with the square hole 801, the right side surface of the shell 1 is provided with a side door 11, the lower side edge position of the side door 11 is hinged with a cover plate 12 through a hinge, the upper end of the cover plate 12 is connected with the side surface of the shell 1 through a hasp 15, the outer side surface of the cover plate 12 is provided with a handle 13, the inner side surface of the cover plate 12 is provided with a rubber cushion 14, the right side surface of the inner cavity of the shell 1 is provided with a temperature and humidity sensor 18 and an air pressure sensor 19, the upper surface of the inner cavity of the shell 1 is provided with a lamp 20, the outer side of the upper surface of the shell 1 is fixedly provided with an electric push rod 21 in the vertical direction, a placing dish 22 is fixedly arranged on an upper end telescopic arm of the electric push rod 21, the upper side opening of the placing dish 22 is provided with a glass cover 23, a bottom interface of the placing dish 22 is provided with a culture tube 24 in the vertical direction, the culture tube 24 penetrates through a corresponding preformed hole on the upper surface of the shell 1, a second electromagnetic valve 25 and a first negative pressure pump 26 are arranged in the middle of the culture tube 24, an L-shaped inoculation tube 27 is fixed on the side surface of the lower end of the culture tube 24, a second negative pressure pump 28 and a third electromagnetic valve 29 are arranged in the middle of the inoculation tube 27, a connecting seat 30 is fixed on the vertical part on the left side of the inoculation tube 27, a flushing tube 31 is arranged in a shaft hole of the connecting seat 30, two positioning rings 36 which are respectively attached to the upper end surface and the lower end surface of the connecting seat 30 are fixed on the lower end of the flushing tube 31, the upper end of the flushing tube 31 penetrates through the preformed hole on the corresponding position of the shell 1, stirring blades 35 are radially distributed on the side surface of the lower end of the flushing tube 31, microbial bacteria to be stirred can rotate to the lower part of the flushing tube 31 along with the turntable 7, a second motor 33 works, the flushing tube 31 is driven to rotate by the matching of the tooth column 34 and the gear sleeve 32, the stirring blades 35 rotate along with the stirring blades to stir the liquid culture medium, the input end of the PLC controller 2 is respectively and electrically connected with the output ends of an external power supply, a temperature and humidity sensor 18 and an air pressure sensor 19, the output end of the PLC controller 2 is respectively and electrically connected with the input ends of a vacuum pump 401, an electric telescopic rod 5, a first motor 9, a lamp 20, an electric push rod 21, a first electromagnetic valve 402, a second electromagnetic valve 25, a first negative pressure pump 26, a second negative pressure pump 28 and a third electromagnetic valve 29, the upper surface of the shell 1 is provided with a second motor 33, a vertical toothed column 34 is fixed on the output shaft of the second motor 33, the toothed column 34 is meshed with a gear sleeve 32 at the upper end of a flushing pipe 31, the input end of the second motor 33 is electrically connected with the output end of the PLC controller 2, the right side surface of the shell 1 is in transition connection with an extension cover 16, the inner side of the upper surface of the extension cover 16 is provided with a negative ion generator 17, the input end of the negative ion generator 17 is electrically connected with the output end of the PLC controller 2, the gas in the inner cavity of the shell 1 is subjected to circulation control through a circulation pipe 301 of a temperature control purification system 3, the circulated gas can be heated through a heater 303, the ambient gas can be cooled through the cooperation of a heat exchanger 304 and a fan 305, the ambient pressure is controlled through a vacuum pump 401, bacterial colonies are in an environment with proper temperature and pressure, a translational mounting frame 6 and a rotary table 7 are arranged at the bottom of the inner cavity of the shell 1, a liquid culture medium and a solid culture medium can be fixed on a clamping seat 701, inoculation operation is convenient, the rotary table 7 and a chuck 8 can be drawn to the outer side of the shell 1, the culture medium is convenient to fix, an anion generator 17 can interfere with the external operation environment, dust can be suppressed, dust can be prevented from entering when the culture medium is fixed, microorganism bacteria are coated through a liftable culture pipe 24 which is matched with a rotatable clamping seat 701, inoculation transfer between the liquid culture medium and the solid culture medium can be carried out through an inoculation pipe 27, can wash the microorganism fungus of solid medium through flushing pipe 31, make things convenient for the absorption of inoculation pipe 27 to shift, flushing pipe 31 can drive stirring piece 35 and rotate, thereby make the bacterial colony distribute evenly, the electronic component that this microorganism fungus culture apparatus adopted is prior art and not innovate here, no longer describe here, PLC controller 2 adopts 6ES 7211-0 AA23-0XB0, this microorganism fungus culture apparatus, the cultivation and the inoculation of microorganism fungus are all arranged in shell 1 and are operated, have good closure, can control the temperature and the cleanliness factor of bacterial colony environment through control by temperature change clean system 3, control atmospheric pressure through vacuum pump 401, carry out the inoculation of microorganism fungus through the cooperation of culture pipe 24, inoculation pipe 27 and flushing pipe 31, guarantee that the culture environment of microorganism fungus is controllable all the time, guarantee the quality of cultivateing.
When in use: rotating the turntable 7 to rotate the notch 802 to the left, controlling the electric telescopic rod 5 to push the mounting rack 6 to the right, enabling the turntable 7 and the chuck 8 to slide out of the side door 11 rightwards, at the moment, the square shaft 10 is separated from the square hole 801 and the notch 802, controlling the anion generator 17 to work, enabling the gas with anions to move downwards to cover the upper side of the turntable 7, relatively mounting the vessel with the solid culture medium and the liquid culture medium on a clamping seat 701 of the turntable 7, after the mounting is completed, pulling the mounting rack 6 and the turntable 7 into the shell 1 by the work of the electric telescopic rod 5, enabling the square shaft 10 to be clamped in the square hole 801, closing the cover plate 12 and locking the cover plate by a hasp 15, transmitting detected temperature, humidity and air pressure data to the PLC controller 2 by the temperature and humidity sensor 18 and the air pressure sensor 19 respectively, when the temperature is higher, the PLC controller 2 drives the fan 305 to work, cooling the air flow passing through the heat exchanger 304, and the PLC controller 2 drives the heater 303 to work when the temperature is raised, heating the air flow passing through the heater 303, when the air pressure is increased, driving the vacuum pump 401 to work, pumping out the air in the shell 1, reducing the air pressure in the shell 1, driving the first electromagnetic valve 402 to close after the adjustment is finished, when inoculating, enabling the solid culture medium and the liquid culture medium to respectively correspond to the flushing pipe 31 and the culture pipe 24, connecting the upper end of the flushing pipe 31 with external flushing equipment, enabling flushing liquid to enter the solid culture medium through the flushing pipe 31, then opening the third electromagnetic valve 29 and the second negative pressure pump 28, enabling the flushed microorganism bacteria to enter the liquid culture medium through the inoculation pipe 27, when directly coating, enabling the culture medium to be positioned at the lower side of the culture pipe 24, placing the microorganism bacteria in the placing dish 22, opening the second electromagnetic valve 25 and the first negative pressure pump 26, simultaneously driving the culture pipe 24 to descend by the electric push rod 21, enabling the lower end of the culture pipe 24 to be in contact with the culture medium, and simultaneously enabling the first motor 9 to work, the chuck 8 is driven to rotate by the matching of the square shaft 10 and the square hole 801, and the culture medium on the rotary disc 7 rotates with the chuck 8 in a small range, so that the microorganism bacteria are coated on the culture medium.
The invention has simple structure and convenient use, can effectively control the environment of bacterial colonies, seals the operation, improves the stability of culture, automatically inoculates, is convenient to operate and improves the quality of microbial bacteria culture.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. A microbial cultivation device comprising a housing (1), characterized in that: the left upper side of shell (1) is equipped with the fixed plate, and PLC controller (2) are installed to the leading flank of fixed plate, the left surface of shell (1) is equipped with temperature control clean system (3), be equipped with negative pressure pipe (4) on the left surface interface of shell (1), the left end import of negative pressure pipe (4) is equipped with vacuum pump (401), the middle part of negative pressure pipe (4) is equipped with first solenoid valve (402), shell (1) inner chamber left surface is fixed with the electric telescopic handle (5) of horizontal direction, be fixed with horizontally mounting bracket (6) on the flexible arm in right side of electric telescopic handle (5), the lower surface of mounting bracket (6) is equipped with gyro wheel (601), the circular logical inslot of mounting bracket (6) right-hand member is equipped with carousel (7), the lower terminal surface of carousel (7) is fixed with chuck (8), the right-hand member of shell (1) inner chamber bottom surface is equipped with first motor (9), the utility model discloses a culture dish, including first motor (9), be fixed with on the output shaft of first motor (9) upside with chuck (8) complex square shaft (10), the right flank of shell (1) is equipped with side door (11), the downside border position of side door (11) articulates through the hinge has apron (12), the upper end of apron (12) is passed through hasp (15) and is connected with the side of shell (1), the inner chamber right flank of shell (1) is equipped with temperature and humidity sensor (18) and baroceptor (19), the inner chamber upper surface of shell (1) is equipped with lamps and lanterns (20), the upper surface outside of shell (1) is fixed with electric putter (21) of vertical direction, be fixed with on the flexible arm in upper end of electric putter (21) and place dish (22), the upside opening department of placing dish (22) is equipped with glass lid (23), be equipped with culture tube (24) of vertical direction on the bottom interface of placing dish (22), cultivate the preformed hole that pipe (24) passed shell (1) upper surface and correspond, the middle part of cultivating pipe (24) is equipped with second solenoid valve (25) and first negative pressure pump (26), the lower extreme side of cultivating pipe (24) is fixed with inoculation pipe (27) of "L" type, the middle part of inoculation pipe (27) is equipped with second negative pressure pump (28) and third solenoid valve (29), the left vertical portion of inoculation pipe (27) is fixed with connecting seat (30), be equipped with flushing pipe (31) in the shaft hole of connecting seat (30), the lower extreme of flushing pipe (31) is fixed with two holding ring one (36) of laminating with terminal surface about connecting seat (30) respectively, the preformed hole that shell (1) corresponds the position is passed to the upper end of flushing pipe (31), the lower extreme side radial distribution of flushing pipe (31) has stirring piece (35), the input of PLC controller (2) respectively with external power source, The output ends of the temperature and humidity sensor (18) and the air pressure sensor (19) are electrically connected, and the output end of the PLC (2) is respectively electrically connected with the input ends of a vacuum pump (401), an electric telescopic rod (5), a first motor (9), a lamp (20), an electric push rod (21), a first electromagnetic valve (402), a second electromagnetic valve (25), a first negative pressure pump (26), a second negative pressure pump (28) and a third electromagnetic valve (29); the right side surface of the shell (1) is connected with an extension cover (16) in a transition mode, a negative ion generator (17) is arranged on the inner side of the upper surface of the extension cover (16), and the input end of the negative ion generator (17) is electrically connected with the output end of the PLC (2); the chuck (8) and the turntable (7) are coaxial, and a square hole (801) matched with the square shaft (10) is formed in the axis of the chuck (8); the circumference of the chuck (8) is provided with a notch (802), and the inner side end of the notch (802) extends to one edge of the square hole (801) and is communicated with the square hole (801).
2. The microorganism culture apparatus according to claim 1, wherein: the temperature control purification system (3) comprises a circulating pipe (301), two ends of the circulating pipe (301) are connected with two interfaces on the upper side and the lower side of the left side surface of the shell (1) respectively, a circulating pump (302) and a heater (303) are arranged in the middle of the circulating pipe (301), and input ends of the circulating pump (302) and the heater (303) are electrically connected with an output end of the PLC (2).
3. The microbial culture device according to claim 2, wherein: the right end of the circulating pipe (301) is provided with a heat exchanger (304), the left side of the shell (1) is provided with a fan (305) at a position corresponding to the heat exchanger (304), and the input end of the fan (305) is electrically connected with the output end of the PLC (2).
4. The microorganism culture apparatus according to claim 3, wherein: the middle part of circulating pipe (301) is equipped with electromagnetism three-way valve (306), and electromagnetism three-way valve (306) are in the upside of circulating pump (302), the side interface of electromagnetism three-way valve (306) links to each other with the gas outlet of atomizer (307), the upper surface of atomizer (307) is equipped with water injection pipe (308), the input of electromagnetism three-way valve (306) and atomizer (307) is connected with the output electricity of PLC controller (2) respectively.
5. The microbial culture device according to claim 1, wherein: the side of carousel (7) is fixed with holding ring two (702), and holding ring two (702) are in the upside of mounting bracket (6), carousel (7) upper surface is equipped with holder (701) relatively.
6. The microbial culture device according to claim 1, wherein: the outer side surface of the cover plate (12) is provided with a handle (13), and the inner side surface of the cover plate (12) is provided with a rubber mat (14).
7. The microbial culture device according to claim 1, wherein: the upper surface of shell (1) is equipped with second motor (33), be fixed with vertical direction's tooth post (34) on the output shaft of second motor (33), tooth post (34) and gear sleeve (32) the meshing of flushing pipe (31) upper end, the input of second motor (33) is connected with the output electricity of PLC controller (2).
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| CN114480097B (en) * | 2022-02-09 | 2023-12-29 | 青岛海信日立空调系统有限公司 | Ion generator microorganism purifying effect testing device |
| CN114464407A (en) * | 2022-02-23 | 2022-05-10 | 安徽飞晶电气设备有限公司 | Moisture absorption device of frame-type amorphous alloy dry-type transformer and use method thereof |
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