CN110241000A - A kind of microbial bacteria culture apparatus - Google Patents
A kind of microbial bacteria culture apparatus Download PDFInfo
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- CN110241000A CN110241000A CN201910402660.5A CN201910402660A CN110241000A CN 110241000 A CN110241000 A CN 110241000A CN 201910402660 A CN201910402660 A CN 201910402660A CN 110241000 A CN110241000 A CN 110241000A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/20—Heat exchange systems, e.g. heat jackets or outer envelopes the heat transfer medium being a gas
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
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Abstract
The invention discloses a kind of microbial bacteria culture apparatus, including shell, the upper left side of the shell is equipped with fixed plate, the leading flank of fixed plate is equipped with PLC controller, the left side of the shell is equipped with temperature control purification system, the left side interface of the shell is equipped with negative tube, the left end import of the negative tube is equipped with vacuum pump, the middle part of negative tube is equipped with the first solenoid valve, the shell inner cavity left side is fixed with the electric telescopic rod of horizontal direction, horizontal mounting rack is fixed on the right side telescopic arm of electric telescopic rod, this microbial bacteria culture apparatus, the culture of microbial bacteria and inoculation are placed in shell and operated, with good closure, it can control the temperature and cleanliness of bacterium colony environment by temperature control purification system, pass through vacuum pump control pressure, pass through culture tube, the cooperation of inoculated tube and flushing pipe carries out micro- The inoculation of biological bacteria guarantees that the culture environment of microbial bacteria is controllable always, guarantees the quality of culture.
Description
Technical field
The present invention relates to field of microbial culture technology, specially a kind of microbial bacteria culture apparatus.
Background technique
The life of current microbial bacteria and the mankind is closely bound up, therefore needs that incubator is used to fill when studying it
It sets, in the production of microbial pesticide, needs mass propgation biological inoculum, after culture medium inoculated vaccine, by stirring, and
Temperature appropriate and oxygen are provided, strain is constantly bred, but the operation of the prior art is by manual realization, and it is cumbersome, hold
It easily makes a fault, the environment of bacterium colony changes when operation, and the inoculation of fluid nutrient medium to solid medium is inconvenient to control, and needs
More experience is wanted, while environmental Kuznets Curves are complicated, improve the cost of culture.
Summary of the invention
The technical problem to be solved by the present invention is to overcome the existing defects, provides a kind of microbial bacteria culture apparatus, structure
Simply, easy to use, effectively the environment of bacterium colony can be controlled, " locked in " operation improves the stability of culture, connect automatically
Kind, it is easy to operate, the quality of microbial bacteria culture is improved, the problems in background technique can be effectively solved.
To achieve the above object, the invention provides the following technical scheme: a kind of microbial bacteria culture apparatus, including shell,
The upper left side of the shell is equipped with fixed plate, and the leading flank of fixed plate is equipped with PLC controller, and the left side of the shell is equipped with
Temperature control purification system, the left side interface of the shell are equipped with negative tube, and the left end import of the negative tube is equipped with vacuum pump,
The middle part of the negative tube is equipped with the first solenoid valve, and the shell inner cavity left side is fixed with the electric telescopic rod of horizontal direction,
Horizontal mounting rack is fixed on the right side telescopic arm of the electric telescopic rod, the lower surface of mounting rack is equipped with idler wheel, the peace
It shelves and is equipped with turntable in the round through slot of right end, the lower end surface of the turntable is fixed with chuck, the right side of the shell inner cavity bottom surface
End is equipped with first motor, and the square shaft with chuck cooperation, the right side of the shell are fixed on the output shaft on the upside of the first motor
Side is equipped with side door, and the lower edge position of the side door is hinged with cover board by hinge, and the upper end of the cover board passes through hasp
It is connect with the side of shell, the inner cavity right side of the shell is equipped with Temperature Humidity Sensor and baroceptor, the shell
Inner cavity upper surface is equipped with lamps and lanterns, and the electric pushrod of vertical direction, the electric pushrod are fixed on the outside of the upper surface of the shell
Telescopic upper end arm on be fixed with placement ware, the upside open-mouth for placing ware is equipped with glass cover, the bottom for placing ware
Interface is equipped with the culture tube of vertical direction, and the culture tube passes through the corresponding preformed hole of upper surface of outer cover, the culture tube
Middle part is equipped with second solenoid valve and the first negative pressure pump, and the lower end side of the culture tube is fixed with the inoculated tube of " L " type, described to connect
The middle part of kind pipe is equipped with the second negative pressure pump and third solenoid valve, and the vertical portion on the left of the inoculated tube is fixed with attachment base, institute
It states and is equipped with flushing pipe in the axis hole of attachment base, the lower end of the flushing pipe is fixed, and there are two be bonded with attachment base upper and lower end face respectively
Positioning ring, the upper end of the flushing pipe passes through the preformed hole of shell corresponding position, and the lower end side of the flushing pipe is radially point
It is furnished with stirring sheet, the output with external power supply, Temperature Humidity Sensor and baroceptor respectively of the input terminal of the PLC controller
End electrical connection, the output end of the PLC controller respectively with vacuum pump, electric telescopic rod, first motor, lamps and lanterns, electric pushrod,
The input terminal electrical connection of first solenoid valve, second solenoid valve, the first negative pressure pump, the second negative pressure pump and third solenoid valve.
As a preferred technical solution of the present invention, the temperature control purification system includes circulation pipe, the circulation pipe
Both ends are connected with two interfaces of shell left side upper and lower sides respectively, and the middle part of the circulation pipe is equipped with circulating pump and heater,
The input terminal of the circulating pump and heater is electrically connected with the output end of PLC controller.
As a preferred technical solution of the present invention, the right end of the circulation pipe is equipped with heat exchanger, the shell left side
The position that face corresponds to heat exchanger is equipped with blower, and the input terminal of the blower is electrically connected with the output end of PLC controller.
As a preferred technical solution of the present invention, the middle part of the circulation pipe is equipped with three-way solenoid valve, and electromagnetism three
Port valve is in the upside of circulating pump, and the side face port of the three-way solenoid valve is connected with the gas outlet of atomizer, the atomizer
Upper surface be equipped with water injection pipe, the input terminal of the three-way solenoid valve and atomizer is electrically connected with the output end of PLC controller respectively
It connects.
As a preferred technical solution of the present invention, the side of the turntable is fixed with positioning ring, and positioning ring is in
The upside of mounting rack, the disk upper surface is opposite to be equipped with holder.
As a preferred technical solution of the present invention, the chuck and turntable coaxial line, the axle center of the chuck is equipped with
With the square hole of square shaft cooperation.
As a preferred technical solution of the present invention, the periphery of the chuck is equipped with short slot, the short slot it is interior
Side extends to a line of square hole and is connected to square hole.
As a preferred technical solution of the present invention, the lateral surface of the cover board is equipped with handle, the inside of the cover board
Face is equipped with rubber mat.
As a preferred technical solution of the present invention, the shell right side transition is connected with extension cover, the extension
Anion generator, the input terminal of the anion generator and the output end electricity of PLC controller are equipped on the inside of the upper surface of cover
Connection.
As a preferred technical solution of the present invention, the upper surface of the shell is equipped with the second motor, second electricity
The tooth post of vertical direction is fixed on the output shaft of machine, the tooth post is engaged with the geared sleeve of flushing pipe upper end, second electricity
The input terminal of machine is electrically connected with the output end of PLC controller.
Compared with prior art, the beneficial effects of the present invention are:
1) culture of microbial bacteria and inoculation are placed in shell and operate by this microbial bacteria culture apparatus, have good
Closure, can control the temperature and cleanliness of bacterium colony environment by temperature control purification system, by vacuum pump control pressure, lead to
The cooperation for crossing culture tube, inoculated tube and flushing pipe carries out the inoculation of microbial bacteria, guarantees that the culture environment of microbial bacteria always may be used
Control guarantees the quality of culture；
2) this microbial bacteria culture apparatus carries out circulation control to the gas of shell inner cavity by the circulation pipe of temperature control purification system
System, can heat the gas of circulation by heater, can be dropped to environmental gas by the cooperation of heat exchanger and blower
Temperature controls environmental stress by vacuum pump, so that bacterium colony is in the environment of preference temperature and pressure；
3) this microbial bacteria culture apparatus, bottom of shell inner cavity are equipped with the mounting rack and turntable of translation, can fix liquid on holder
Culture medium and solid medium facilitate and carry out inoculation operation, turntable and chuck can pull to the outside of shell, facilitate culture medium
Fixed, anion generator can interfere external operating environment, can dust suppression, dust enters when avoiding culture medium fixed；
4) this microbial bacteria culture apparatus cooperates the painting of rotatable holder progress microbial bacteria by liftable culture tube
Cloth can carry out the transfer of the inoculation between fluid nutrient medium and solid medium by inoculated tube, can be trained to solid by flushing pipe
The microbial bacteria for supporting base is rinsed, and facilitates the absorption of inoculated tube to shift, flushing pipe can drive stirring sheet to rotate, to make bacterium colony
It is evenly distributed.
Detailed description of the invention
Fig. 1 is the whole the schematic diagram of the section structure of the present invention；
Fig. 2 is structure enlargement diagram at A of the present invention；
Fig. 3 is chuck structure schematic diagram of the present invention.
In figure: 1 shell, 2 PLC controllers, 3 temperature control purification systems, 301 circulation pipes, 302 circulating pumps, 303 heaters,
304 heat exchangers, 305 blowers, 306 triple valves, 307 atomizers, 308 water injection pipes, 4 negative tubes, 401 vacuum pumps, 402 first electromagnetism
Valve, 5 electric telescopic rods, 6 mounting racks, 601 idler wheels, 7 turntables, 701 holders, 702 positioning rings, 8 chucks, 801 square holes, 802 short slots,
9 first motors, 10 square shafts, 11 side doors, 12 cover boards, 13 handles, 14 rubber mats, 15 hasps, 16 extend covers, 17 anion generators,
Wares, 23 glass covers, 24 culture tubes, 25 the are placed in 18 Temperature Humidity Sensors, 19 baroceptors, 20 lamps and lanterns, 21 electric pushrods, 22
Two solenoid valves, 26 first negative pressure pumps, 27 inoculated tubes, 28 second negative pressure pumps, 29 third solenoid valves, 30 attachment bases, 31 flushing pipes, 32
Geared sleeve, 33 second motors, 34 tooth posts, 35 stirring sheets, 36 positioning rings.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution: a kind of microbial bacteria culture apparatus, including shell 1, outside
The upper left side of shell 1 is equipped with fixed plate, and the leading flank of fixed plate is equipped with PLC controller 2, and it is net that the left side of shell 1 is equipped with temperature control
Change system 3, temperature control purification system 3 include circulation pipe 301, the both ends of circulation pipe 301 respectively with 1 left side upper and lower sides of shell two
A interface is connected, and the middle part of circulation pipe 301 is equipped with circulating pump 302 and heater 303, the input of circulating pump 302 and heater 303
End is electrically connected with the output end of PLC controller 2, and the right end of circulation pipe 301 is equipped with heat exchanger 304, the corresponding heat exchange in 1 left side of shell
The position of device 304 is equipped with blower 305, and the input terminal of blower 305 is electrically connected with the output end of PLC controller 2, circulation pipe 301
Middle part is equipped with three-way solenoid valve 306, and three-way solenoid valve 306 is in the upside of circulating pump 302, the side of three-way solenoid valve 306
Interface is connected with the gas outlet of atomizer 307, and the upper surface of atomizer 307 is equipped with water injection pipe 308, three-way solenoid valve 306 and mist
The input terminal for changing device 307 is electrically connected with the output end of PLC controller 2 respectively, and the left side interface of shell 1 is equipped with negative tube 4,
The left end import of negative tube 4 is equipped with vacuum pump 401, and the middle part of negative tube 4 is equipped with the first solenoid valve 402,1 inner cavity left side of shell
It is fixed with the electric telescopic rod 5 of horizontal direction, horizontal mounting rack 6 is fixed on the right side telescopic arm of electric telescopic rod 5, is installed
The lower surface of frame 6 is equipped with idler wheel 601, is equipped with turntable 7 in the round through slot of 6 right end of mounting rack, the side of turntable 7 is fixed with positioning
Ring 702, and positioning ring 702 is in the upside of mounting rack 6,7 upper surface of turntable is opposite to be equipped with holder 701, and the lower end surface of turntable 7 is solid
Surely there is chuck 8, the right end of 1 inner chamber bottom surface of shell is equipped with first motor 9, is fixed with and blocks on the output shaft of 9 upside of first motor
The square shaft 10 that disk 8 cooperates, chuck 8 and 7 coaxial line of turntable, the axle center of chuck 8 are equipped with the square hole 801 cooperated with square shaft 10, chuck 8
Periphery be equipped with short slot 802, the inner end of short slot 802 extends to a line of square hole 801 and is connected to square hole 801, outside
The right side of shell 1 is equipped with side door 11, and the lower edge position of side door 11 is hinged with cover board 12 by hinge, and the upper end of cover board 12 is logical
Hasp 15 to be crossed to connect with the side of shell 1, the lateral surface of cover board 12 is equipped with handle 13, and the medial surface of cover board 12 is equipped with rubber mat 14,
The inner cavity right side of shell 1 is equipped with Temperature Humidity Sensor 18 and baroceptor 19, and the inner cavity upper surface of shell 1 is equipped with lamps and lanterns
20, the upper surface outside of shell 1 is fixed with the electric pushrod 21 of vertical direction, is fixed on the telescopic upper end arm of electric pushrod 21
Ware 22 is placed, the upside open-mouth for placing ware 22 is equipped with glass cover 23, and the bottom interface for placing ware 22 is equipped with vertical direction
Culture tube 24, culture tube 24 pass through the corresponding preformed hole in 1 upper surface of shell, and the middle part of culture tube 24 is equipped with 25 He of second solenoid valve
First negative pressure pump 26, the lower end side of culture tube 24 are fixed with the inoculated tube 27 of " L " type, and it is negative that the middle part of inoculated tube 27 is equipped with second
The vertical portion of press pump 28 and third solenoid valve 29,27 left side of inoculated tube is fixed with attachment base 30, sets in the axis hole of attachment base 30
There is flushing pipe 31, the lower end of flushing pipe 31 is fixed, and there are two the positioning rings 36 being bonded respectively with 30 upper and lower end face of attachment base, rinse
The upper end of pipe 31 passes through the preformed hole of 1 corresponding position of shell, and the lower end side of flushing pipe 31 is radially distributed with stirring sheet 35, need to stir
The microbial bacteria mixed can go to the lower section of flushing pipe 31 with turntable 7, the work of the second motor 33, pass through tooth post 34 and geared sleeve 32
Cooperation drives flushing pipe 31 to rotate, and stirring sheet 35, which rotates with it, is stirred fluid nutrient medium, the input terminal of PLC controller 2
It is electrically connected respectively with the output end of external power supply, Temperature Humidity Sensor 18 and baroceptor 19, the output end of PLC controller 2
It is electric with vacuum pump 401, electric telescopic rod 5, first motor 9, lamps and lanterns 20, electric pushrod 21, the first solenoid valve 402, second respectively
The input terminal electrical connection of magnet valve 25, the first negative pressure pump 26, the second negative pressure pump 28 and third solenoid valve 29, the upper surface of shell 1 is set
There is the second motor 33, the tooth post 34 of vertical direction, tooth post 34 and 31 upper end of flushing pipe are fixed on the output shaft of the second motor 33
Geared sleeve 32 engage, the input terminal of the second motor 33 is electrically connected with the output end of PLC controller 2,1 right side transition of shell company
Be connected to extension cover 16, extend cover 16 upper surface on the inside of be equipped with anion generator 17, the input terminal of anion generator 17 with
The output end of PLC controller 2 is electrically connected, and is followed by the circulation pipe 301 of temperature control purification system 3 to the gas of 1 inner cavity of shell
Ring control, can heat the gas of circulation by heater 303, can be to ring by the cooperation of heat exchanger 304 and blower 305
Border gas cools down, and is controlled by vacuum pump 401 environmental stress, so that bacterium colony is in the ring of preference temperature and pressure
In border, 1 intracavity bottom of shell is equipped with the mounting rack 6 and turntable 7 of translation, and fluid nutrient medium and solid training can be fixed on holder 701
Support base, facilitate and carry out inoculation operation, turntable 7 and chuck 8 can pull to the outside of shell 1, facilitate the fixation of culture medium, anion
Generator 17 can interfere external operating environment, can dust suppression, dust enters when avoiding culture medium fixed, passes through liftable
Culture tube 24 cooperate rotatable holder 701 to carry out the coating of microbial bacteria, fluid nutrient medium can be carried out by inoculated tube 27
Inoculation transfer between solid medium, can be rinsed the microbial bacteria of solid medium by flushing pipe 31, convenient
The absorption of inoculated tube 27 is shifted, and flushing pipe 31 can drive stirring sheet 35 to rotate, so that bacterium colony is made to be evenly distributed, the training of this microbial bacteria
It supports electronic component used by device to be the prior art and do not innovated herein, no longer be repeated herein, PLC control
Device 2 uses 6ES7 211-0AA23-0XB0, and the culture of microbial bacteria and inoculation are placed in outer by this microbial bacteria culture apparatus
It is operated in shell 1, there is good closure, can control the temperature and cleaning of bacterium colony environment by temperature control purification system 3
Degree carries out microbial bacteria by the cooperation of culture tube 24, inoculated tube 27 and flushing pipe 31 by 401 control pressure of vacuum pump
Inoculation, guarantees that the culture environment of microbial bacteria is controllable always, guarantees the quality of culture.
When in use: rotary turnplate 7 makes short slot 802 go to left side, and control electric telescopic rod 5 pushes to the right mounting rack 6, makes
It obtains turntable 7 and chuck 8 skids off to the right side door 11, square shaft 10 is detached from square hole 801 and short slot 802 at this time, controls anion generator
17 work, the gas with anion moves down the top of covering turntable 7, will be with solid medium and fluid nutrient medium
Vessel are opposite to be mounted on the holder 701 of turntable 7, and after being installed, the work of electric telescopic rod 5 pulls in mounting rack 6 and turntable 7
In shell 1, so that square shaft 10 is stuck in square hole 801, closes cover board 12 and locked by hasp 15, Temperature Humidity Sensor 18 is gentle
Pressure sensor 19 will test temperature and humidity respectively and barometric information is transferred to PLC controller 2, when temperature is higher, PLC controller 2
It drives blower 305 to work, cools down to by the air-flow of heat exchanger 304, PLC controller 2 drives heater when temperature is higher
303 work, are heated to by the air-flow of heater 303, and when air pressure is higher, driving vacuum pump 401 works, will be in shell 1
Gas extraction, reduce the air pressure in shell 1, the first solenoid valve 402 driven to close after adjusting, when inoculation, train solid
It supports base and fluid nutrient medium respectively corresponds flushing pipe 31 and culture tube 24, flushing device outside the upper termination of flushing pipe 31 rinses
Liquid enters in solid medium by flushing pipe 31, and third solenoid valve 29 and the second negative pressure pump 28 are opened later, after flushing
Microbial bacteria enters in fluid nutrient medium by inoculated tube 27, when being directly coated, culture medium is made to be in culture tube 24
Microbial bacteria is placed in and places in ware 22 by downside, and second solenoid valve 25 and the first negative pressure pump 26 are opened, while 21 band of electric pushrod
Dynamic culture tube 24 declines, and contacts the lower end of culture tube 24 with culture medium, while first motor 9 works, and passes through square shaft 10 and side
The cooperation in hole 801 drives chuck 8 to rotate, and the culture medium on turntable 7 carries out rotation by a small margin with chuck 8, so that microbial bacteria
It is coated on culture medium.
The configuration of the present invention is simple, it is easy to use, effectively the environment of bacterium colony can be controlled, " locked in " operation, improve culture
Stability, be inoculated with automatically, it is easy to operate, improve microbial bacteria culture quality.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (10)
1. a kind of microbial bacteria culture apparatus, including shell (1), it is characterised in that: the upper left side of the shell (1), which is equipped with, to be fixed
Plate, the leading flank of fixed plate are equipped with PLC controller (2), and the left side of the shell (1) is equipped with temperature control purification system (3), institute
The left side interface for stating shell (1) is equipped with negative tube (4), and the left end import of the negative tube (4) is equipped with vacuum pump (401),
The middle part of the negative tube (4) is equipped with the first solenoid valve (402), and shell (1) the inner cavity left side is fixed with horizontal direction
Electric telescopic rod (5) is fixed with horizontal mounting rack (6) on the right side telescopic arm of the electric telescopic rod (5), mounting rack (6)
Lower surface be equipped with idler wheel (601), be equipped with turntable (7) in the round through slot of mounting rack (6) right end, the turntable (7)
Lower end surface is fixed with chuck (8), and the right end of shell (1) inner chamber bottom surface is equipped with first motor (9), the first motor (9)
The square shaft (10) with chuck (8) cooperation is fixed on the output shaft of upside, the right side of the shell (1) is equipped with side door (11),
The lower edge position of the side door (11) is hinged with cover board (12) by hinge, and the upper end of the cover board (12) passes through hasp
(15) it is connect with the side of shell (1), the inner cavity right side of the shell (1) is equipped with Temperature Humidity Sensor (18) and air pressure sensing
The inner cavity upper surface of device (19), the shell (1) is equipped with lamps and lanterns (20), and it is vertical to be fixed on the outside of the upper surface of the shell (1)
The electric pushrod (21) in direction is fixed on the telescopic upper end arm of the electric pushrod (21) and places ware (22), the placement ware
(22) upside open-mouth is equipped with glass cover (23), and the bottom interface for placing ware (22) is equipped with the culture tube of vertical direction
(24), the culture tube (24) passes through the corresponding preformed hole in shell (1) upper surface, and the middle part of the culture tube (24) is equipped with second
The lower end side of solenoid valve (25) and the first negative pressure pump (26), the culture tube (24) is fixed with the inoculated tube (27) of " L " type, institute
The middle part for stating inoculated tube (27) is equipped with the second negative pressure pump (28) and third solenoid valve (29), vertical on the left of the inoculated tube (27)
Part is fixed with attachment base (30), is equipped with flushing pipe (31) in the axis hole of the attachment base (30), under the flushing pipe (31)
End is fixed there are two the positioning ring (36) being bonded respectively with attachment base (30) upper and lower end face, and the upper end of the flushing pipe (31) passes through
The lower end side of the preformed hole of shell (1) corresponding position, the flushing pipe (31) is radially distributed with stirring sheet (35), the PLC
Output end of the input terminal of controller (2) respectively with external power supply, Temperature Humidity Sensor (18) and baroceptor (19) is electrically connected
Connect, the output end of the PLC controller (2) respectively with vacuum pump (401), electric telescopic rod (5), first motor (9), lamps and lanterns
(20), electric pushrod (21), the first solenoid valve (402), second solenoid valve (25), the first negative pressure pump (26), the second negative pressure pump
(28) it is electrically connected with the input terminal of third solenoid valve (29).
2. a kind of microbial bacteria culture apparatus according to claim 1, it is characterised in that: the temperature control purification system (3)
Including circulation pipe (301), the both ends of the circulation pipe (301) are connected with two interfaces of shell (1) left side upper and lower sides respectively,
The middle part of the circulation pipe (301) is equipped with circulating pump (302) and heater (303), the circulating pump (302) and heater
(303) input terminal is electrically connected with the output end of PLC controller (2).
3. a kind of microbial bacteria culture apparatus according to claim 2, it is characterised in that: the right side of the circulation pipe (301)
End is equipped with heat exchanger (304), and the position that shell (1) left side corresponds to heat exchanger (304) is equipped with blower (305), the wind
The input terminal of machine (305) is electrically connected with the output end of PLC controller (2).
4. a kind of microbial bacteria culture apparatus according to claim 3, it is characterised in that: in the circulation pipe (301)
Portion is equipped with three-way solenoid valve (306), and three-way solenoid valve (306) is in the upside of circulating pump (302), the three-way solenoid valve
(306) side face port is connected with the gas outlet of atomizer (307), and the upper surface of the atomizer (307) is equipped with water injection pipe
(308), output end of the input terminal of the three-way solenoid valve (306) and atomizer (307) respectively with PLC controller (2) is electrically connected
It connects.
5. a kind of microbial bacteria culture apparatus according to claim 1, it is characterised in that: the side of the turntable (7) is solid
Surely have positioning ring (702), and positioning ring (702) is in the upside of mounting rack (6), turntable (7) upper surface is opposite to be equipped with folder
Seat (701).
6. a kind of microbial bacteria culture apparatus according to claim 1, it is characterised in that: the chuck (8) and turntable (7)
Coaxial line, the axle center of the chuck (8) are equipped with the square hole (801) cooperated with square shaft (10).
7. a kind of microbial bacteria culture apparatus according to claim 6, it is characterised in that: the periphery of the chuck (8)
It is equipped with short slot (802), the inner end of the short slot (802) extends to a line of square hole (801) and connects with square hole (801)
It is logical.
8. a kind of microbial bacteria culture apparatus according to claim 1, it is characterised in that: the lateral surface of the cover board (12)
Equipped with handle (13), the medial surface of the cover board (12) is equipped with rubber mat (14).
9. a kind of microbial bacteria culture apparatus according to claim 1, it is characterised in that: shell (1) right side mistake
It crosses and is connected with extension cover (16), be equipped with anion generator (17) on the inside of the upper surface for extending cover (16), the anion
The input terminal of generator (17) is electrically connected with the output end of PLC controller (2).
10. a kind of microbial bacteria culture apparatus according to claim 1, it is characterised in that: the upper surface of the shell (1)
Equipped with the second motor (33), the tooth post (34) of vertical direction, the tooth post are fixed on the output shaft of second motor (33)
(34) it is engaged with the geared sleeve (32) of flushing pipe (31) upper end, input terminal and PLC controller (2) of second motor (33)
Output end electrical connection.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910402660.5A CN110241000B (en) | 2019-05-15 | 2019-05-15 | Microbial cultivation device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910402660.5A CN110241000B (en) | 2019-05-15 | 2019-05-15 | Microbial cultivation device |
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| CN110241000B CN110241000B (en) | 2022-05-31 |
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| CN201910402660.5A Active CN110241000B (en) | 2019-05-15 | 2019-05-15 | Microbial cultivation device |
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| CN114480097A (en) * | 2022-02-09 | 2022-05-13 | 青岛海信日立空调系统有限公司 | Little biological purification effect testing arrangement of ion generator |
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| CN110241000B (en) | 2022-05-31 |
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