CN111518674B - Culture system suitable for directional evolution of microorganisms - Google Patents
Culture system suitable for directional evolution of microorganisms Download PDFInfo
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- CN111518674B CN111518674B CN202010505026.7A CN202010505026A CN111518674B CN 111518674 B CN111518674 B CN 111518674B CN 202010505026 A CN202010505026 A CN 202010505026A CN 111518674 B CN111518674 B CN 111518674B
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- 244000005700 microbiome Species 0.000 title claims abstract description 28
- 239000007788 liquid Substances 0.000 claims abstract description 128
- 238000002347 injection Methods 0.000 claims abstract description 56
- 239000007924 injection Substances 0.000 claims abstract description 56
- 238000004321 preservation Methods 0.000 claims abstract description 49
- 238000000855 fermentation Methods 0.000 claims abstract description 24
- 230000004151 fermentation Effects 0.000 claims abstract description 24
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 230000033001 locomotion Effects 0.000 claims description 12
- 238000007789 sealing Methods 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims 3
- 241000894006 Bacteria Species 0.000 abstract description 4
- 238000011109 contamination Methods 0.000 abstract description 4
- 230000000737 periodic effect Effects 0.000 abstract 1
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
- 238000005057 refrigeration Methods 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 230000001502 supplementing effect Effects 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 6
- 239000002699 waste material Substances 0.000 description 5
- 238000010276 construction Methods 0.000 description 3
- 239000008367 deionised water Substances 0.000 description 3
- 229910021641 deionized water Inorganic materials 0.000 description 3
- 239000002360 explosive Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000002572 peristaltic effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000003028 elevating effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention provides a culture system suitable for directional evolution of microorganisms, which can solve the problems of complex strain preservation operation and easy bacteria contamination existing in the current directional evolution of microorganisms. Culture system suitable for microorganism directed evolution, including culture apparatus, culture apparatus includes fermentation cylinder, its characterized in that: the culture system also comprises a strain preservation device, wherein the strain preservation device comprises a pipe rack, a refrigerating mechanism, a pipe cover opening mechanism and a pipe body liquid injection mechanism, and the pipe rack is provided with more than 2 pipe holes for containing preservation pipes; the refrigerating mechanism is used for refrigerating the preservation pipes placed on the pipe rack; the tube cover opening mechanism is used for opening and closing the tube cover of the preservation tube; the pipe body liquid injection mechanism is used for conveying the bacterial liquid in the fermentation tank into the preservation pipe. The culture system can realize periodic and automatic preservation of bacterial liquid, avoids manual operation and reduces the risk of bacterial contamination.
Description
Technical Field
The invention relates to the technical field of microorganisms, in particular to a culture system suitable for directional evolution of microorganisms.
Background
In the process of directional evolution of microorganisms, the conditions for culturing the microorganisms are required to be continuously changed, part of fermentation liquor is required to be taken out at intervals to preserve strains, the manual operation is complex and complicated, bacteria are easy to dye, the evolution effect is poor, meanwhile, manual sampling cannot accurately control time and can possibly be missed or forgotten to take, and if the directional evolution of the microorganisms can be automatically controlled, the working efficiency can be greatly improved, the burden of people is lightened, and the probability of dyeing bacteria is reduced.
Disclosure of Invention
The invention provides a culture system suitable for directional evolution of microorganisms, which can solve the problems of complex strain preservation operation and easy bacteria contamination existing in the current directional evolution of microorganisms.
The technical scheme is that the culture system suitable for directional evolution of microorganisms comprises a culture device, wherein the culture device comprises a fermentation tank and is characterized in that: the culture system also comprises a strain preservation device, wherein the strain preservation device comprises a pipe frame, a refrigerating mechanism, a pipe cover opening mechanism and a pipe body liquid injection mechanism,
The pipe rack is provided with more than 2 pipe holes for holding the preservation pipes;
the refrigerating mechanism is used for refrigerating the preservation pipes placed on the pipe rack;
The tube cover opening mechanism is used for opening and closing the tube cover of the preservation tube;
The pipe body liquid injection mechanism is used for conveying the bacterial liquid in the fermentation tank to the preservation pipe.
Further, the fermentation tank, the refrigerating mechanism, the pipe cover opening mechanism and the pipe body liquid injection mechanism are respectively and electrically connected with the controller.
Further, the pipe frame is of an annular structure, pipe holes are uniformly and alternately distributed on the pipe frame, and the pipe frame is connected with the pipe frame driving unit and rotates around the axis under the action of the pipe frame driving unit;
The pipe cover opening mechanism comprises a pipe cover push rod, a pipe cover lifting arm, a pipe cover motor and a pipe cover clamp, wherein the pipe cover push rod is arranged on one side of the bracket, the output end of the pipe cover push rod is connected with one end of the pipe cover lifting arm and is used for driving the pipe cover lifting arm to do lifting movement, the other end of the pipe cover lifting arm is vertically matched with a pair of guide posts arranged on the bracket, and the pipe cover motor is arranged on the pipe cover lifting arm and is used for driving the pipe cover clamp to do rotary movement in a horizontal plane; preferably, the pipe cover clamping device is of a sleeve structure with an upper end sealing plate and a lower end open, and the side wall of the pipe cover clamping device is provided with more than 2 slits extending from bottom to top to form spring petals for clamping the pipe cover; the inner side wall of the pipe cover clamp is provided with anti-slip rubber;
The pipe body annotates liquid mechanism and includes filling liquid pipeline, valve, delivery pump, liquid push rod, liquid feeding motor, liquid feeding lifting arm, liquid feeding head, annotate liquid pipeline intercommunication liquid joint on fermentation cylinder and the liquid feeding head, annotate liquid pipeline connection the valve with the delivery pump, the liquid feeding push rod is installed in the support opposite side, the liquid feeding motor is connected to the output of liquid feeding push rod and is used for driving the liquid feeding motor and is elevating movement, the one end of liquid feeding lifting arm is connected to the output of liquid feeding motor and is used for driving the liquid feeding lifting arm and is the rotary motion in the horizontal plane, the liquid feeding head is connected to the other end of liquid feeding lifting arm.
Further, the culture system further comprises a tube fixing mechanism, wherein the tube fixing mechanism is used for fixing the tube body of the preservation tube; preferably, the pipe body fixing mechanism comprises a left clamp valve, a right clamp valve and a power source, wherein the left clamp valve and the right clamp valve are respectively provided with an arc-shaped groove matched with the pipe body, the left clamp valve and the right clamp valve are oppositely moved under the action of the power source to clamp the pipe body, and the power source is an air cylinder.
Further, the refrigeration mechanism includes the refrigeration piece and the cold board that passes that are annular structure, refrigeration piece, pass cold board with the backup pad of pipe support connects gradually from bottom to top, refrigeration piece the interior, the outside of passing the cold board are encircleed respectively and are provided with lateral part heat preservation, the upper portion of passing the cold board is provided with upper portion heat preservation, refrigeration piece, pass the cold board be equipped with the tube hole intercommunication and run through the perforation of upper portion heat preservation.
Further, the liquid adding head is sleeved with a sealing ring, the liquid adding head is further provided with a gas connector, the gas connector is connected with a gas source, and a filter element is fixed on the upper portion of the preservation pipe.
Further, the culture system further comprises a base and an outer cover, and the pipe rack, the moving mechanism, the refrigerating mechanism and the pipe cover opening mechanism are all arranged on the base and are covered by the outer cover.
The culture system can automatically realize that bacterial liquid in the fermentation tank is conveyed into the preservation pipe for preservation at regular intervals, and specifically, firstly, the pipe cover opening mechanism rotates and lifts the pipe cover to a fully opened state, then, the pipe body liquid injection mechanism conveys bacterial liquid in the fermentation tank into the preservation pipe, and finally, the pipe cover opening mechanism moves down and rotates the pipe cover to a closed state, so that automatic preservation of bacterial in the process of directional evolution culture of microorganisms is realized, manual operation is avoided, and the risk of bacterial contamination is reduced.
Drawings
FIG. 1 is a schematic perspective view of a culture system according to the present invention.
FIG. 2 is a schematic view of the explosive structures of the upper base, the lower base and the fermenter according to the present invention.
FIG. 3 is a schematic view of an exploded view of the strain-preserving device according to the present invention at a first view angle.
FIG. 4 is a schematic view showing a structure of the strain-preserving device according to the present invention with a cover omitted.
FIG. 5 is a schematic view of an exploded view of a second view of the strain-preserving device of the present invention.
Fig. 6 is a schematic view of an exploded construction of the tube cap opening mechanism of the present invention.
Fig. 7 is a schematic diagram of an exploded structure of the tube liquid injection mechanism of the present invention.
Fig. 8 is a schematic structural view of the pipe fixing mechanism of the present invention.
FIG. 9 is a schematic view of the explosive structure of the preservation tube of the present invention.
Fig. 10 is a schematic of the explosive construction of the tube rack and refrigeration mechanism of the present invention.
Detailed Description
As shown in figures 1-10, the culture system suitable for directional evolution of microorganisms comprises a culture device and a strain preservation device.
The culture device comprises a fermentation tank 21, a reagent bottle 22 and a fermentation tank liquid injection mechanism, wherein the fermentation tank liquid injection mechanism comprises a liquid supplementing pipeline 23 communicated with the reagent bottle and the fermentation tank 21, and a liquid discharging pipeline for outputting bacterial liquid and waste liquid in the fermentation tank 21, wherein the liquid supplementing pipeline 23 and the liquid discharging pipeline are respectively connected with a liquid supplementing valve (pinch valve) and a liquid supplementing conveying pump 24 (peristaltic pump), and the fermentation tank 21, the reagent bottle 22, the liquid supplementing pipeline 23, the liquid supplementing valve and the liquid supplementing conveying pump 24 are connected to be conventional technologies, and are not repeated herein.
The strain preserving device comprises a base, a pipe rack, a culturing device, a refrigerating mechanism, a pipe body fixing mechanism, a pipe cover opening mechanism and a pipe body liquid injection mechanism 70.
The base includes base 11 and lower base 12, and the pipe support is all and is covered through dustcoat 13 to the pipe support, support 14, mobile mechanism, refrigeration mechanism and tube cap opening mechanism of being installed to base 11, and dustcoat 13 and the both sides wall sliding fit of base 11, and the lower part of lower base 12 outwards extends and forms boss 121, is equipped with on the boss 121 with fermentation cylinder complex constant head tank 122.
The pipe frame has offered the tube hole 311 that is used for holding the deposit pipe more than 2, and specifically, the pipe frame is annular structure, and even, the interval distribution has tube hole 311 more than 2 on the pipe frame, and pipe frame connection pipe frame drive unit and rotate around the axis under the effect of pipe frame drive unit for deposit the pipe after the application of sample and leave annotate the liquid station, and empty deposit the pipe and remove annotate the liquid station.
The refrigerating mechanism is used for refrigerating the preservation pipe contained on the pipe rack, and specifically, the refrigerating mechanism comprises a refrigerating sheet 41 (a semiconductor refrigerating sheet 41) and a cold transfer plate 42 which are of annular structures, the refrigerating sheet 41 and the cold transfer plate 42 are arranged from bottom to top and are connected with a supporting plate 31 of the pipe rack through connecting columns distributed in an annular mode, side heat preservation layers 43 are respectively arranged on the inner side and the outer side of the refrigerating sheet 41 and the cold transfer plate 42 in a surrounding mode, an upper heat preservation layer 44 is arranged on the cold transfer plate 42, and the refrigerating sheet 41 and the cold transfer plate 42 are provided with refrigerating sheet perforation 411 and cold transfer plate perforation 421 which are communicated with the pipe holes 311 and penetrate through the upper heat preservation layer 44. The conventional pipe rack includes an upper support plate and a lower support plate connected by a connecting column, and in this embodiment, a stable pipe rack is formed by replacing the lower support plate with the upper support plate by a refrigerating sheet, and an output shaft 32 of a pipe rack driving unit is connected with a connecting frame 412 in the middle of the refrigerating sheet 41.
The tube body fixing mechanism is used for fixing the tube body of the preservation tube; specifically, the pipe body fixing mechanism comprises a left clamp valve 51, a right clamp valve 52 and a power source 53, wherein the left clamp valve 51 and the right clamp valve 52 are respectively provided with an arc-shaped groove matched with the pipe body, the right clamp valve 52 and the left clamp valve 51 are respectively arranged on the inner side and the outer side of the pipe frame, a gap is arranged between the upper heat preservation layer 44 and the supporting plate 31, and the right clamp valve 52, the left clamp valve 51 and the gap are arranged at equal heights. When the tube cover opening mechanism acts, the left clamp valve 51 and the right clamp valve 52 move in opposite directions under the action of the power source and extend into the gap to clamp the tube body, the tube body and the tube cover are prevented from rotating along, and the power source 53 is an air cylinder.
The tube cover opening mechanism 60 is used for opening and closing the tube cover of the preservation tube, and specifically comprises a tube cover push rod 61, a tube cover lifting arm 63, a tube cover motor and a tube cover clamp 62, wherein the tube cover push rod 61 (electric push rod) is installed on one side of the support, the output end of the tube cover push rod 61 is connected with one end of the tube cover lifting arm 63 and is used for driving the tube cover lifting arm 63 to perform lifting motion, the other end of the tube cover lifting arm is vertically matched with a pair of guide posts installed on the support, the tube cover motor is installed on the tube cover lifting arm and is in transmission matching with the tube cover clamp 62 through a gear set to drive the tube cover clamp 62 to perform rotation motion in a horizontal plane, when the tube cover needs to be opened, the tube cover push rod drives the tube cover lifting arm to move downwards, the tube cover motor drives the tube cover clamp to rotate, meanwhile, the tube cover push rod drives the tube cover to be lifted to be completely opened, and the opposite operation is performed when the tube cover needs to be closed. The tube cap clamp 62 has a sleeve structure with an upper end closing plate and a lower end opening, and the side wall of the tube cap clamp 62 is provided with more than 2 slits extending from bottom to top to form spring flaps for clamping the tube cap so as to clamp the tube cap; the inner side wall of the tube cover clamp 62 is provided with anti-slip rubber 64, so that the tube cover is prevented from slipping or falling off, and smooth opening and closing of the tube cover are ensured.
The pipe body liquid injection mechanism is used for conveying bacterial liquid in the fermentation tank into the preservation pipe, and specifically, the pipe body liquid injection mechanism comprises a liquid injection pipeline 77, a liquid injection valve 76 (pinch valve), a liquid injection conveying pump 78 (peristaltic pump), a liquid injection push rod 71 (electric push rod), a liquid injection motor 72, a liquid injection lifting arm 73 and a liquid injection head 74, the fermentation tank, a reagent bottle (a reagent bottle containing sodium hydroxide solution and a reagent bottle containing deionized water) are respectively communicated with a liquid joint 741 on the liquid injection head 74 through the liquid injection pipeline 77, the liquid injection valve 76 and the liquid injection conveying pump 78 are installed on the liquid injection pipeline 77, the liquid injection push rod 71 is installed on the other side of the support, the output end of the liquid injection push rod 71 is connected with the liquid injection motor 72 and is used for driving the liquid injection motor 72 to do lifting motion, the output end of the liquid injection motor 72 is connected with one end of the liquid injection lifting arm 73 and is used for driving the liquid injection lifting arm 73 to do rotary motion in the horizontal plane, and the other end of the liquid injection lifting arm 73 is connected with the liquid injection head 74. Through the switching control of annotating liquid valve, annotate liquid delivery pump, make sodium hydroxide solution, deionized water, the culture medium that contains the microorganism flow through liquid joint respectively, wherein, when using sodium hydroxide solution, deionized water to wash liquid joint, start the liquid feeding motor, make the liquid feeding lifting arm outwards rotate to liquid joint and waste liquid groove just, the liquid feeding push rod drives the liquid feeding motor, the liquid feeding lifting arm descends, make the liquid joint stretch into the upper portion opening of waste liquid groove, waste liquid after the washing flows into the waste liquid groove, after the washing, start the liquid feeding motor and make the liquid feeding lifting arm inwards rotate to liquid feeding head just to preserving the pipe, the liquid feeding push rod drives liquid feeding motor, the liquid feeding lifting arm descends, make the liquid joint stretch into the upper portion opening of body, the culture medium that contains the microorganism flows into preserving the pipe. When the pipe cover opening mechanism acts, the liquid adding motor drives the liquid adding lifting arm to rotate outwards, and mutual interference of the liquid adding lifting arm and the pipe cover opening mechanism can be avoided.
The pipe rack, the refrigerating mechanism, the pipe body fixing mechanism, the pipe cover opening mechanism and the pipe body liquid injection mechanism are arranged, so that the whole structure of the strain preservation device is compact, the volume of the device can be effectively reduced, the mechanical stroke is shortened, and the preservation efficiency is improved.
The preservation pipe 80 includes tube cap 81, intubate 82, filter core and body 83, intubate 82 cartridge in body 83 and through upper portion limit flange and body upper edge butt, the diapire of intubate 82 is hollow out construction, and filter core 84 is installed in the bottom of intubate 82 and is located the upper portion of preserving the pipe, and the liquid feeding head 74 overcoat is equipped with sealing washer 75, bottom is equipped with gas connector 742, and gas connector connects the air supply. The filter element is arranged to filter and separate microorganisms from the culture medium, the microorganisms are remained on the filter element, and the culture medium flows into the lower part of the preservation tube; through the arrangement of the air source, the space at the upper part of the filter element is pressurized, so that the filtration and separation of microorganisms and culture medium are promoted, and the preservation efficiency is further improved; the pressure head is pressed on the upper edge of the tube body of the preservation tube, and the joint of the pressure head and the preservation tube is sealed by a sealing ring. After the microorganism and the culture medium are separated, the components in the microorganism cells and the culture medium are not greatly changed, and relatively accurate sampling point data can be obtained.
The fermentation tank, the fermentation tank liquid injection mechanism, the refrigeration mechanism, the pipe body fixing mechanism, the pipe cover opening mechanism and the pipe body liquid injection mechanism are respectively and electrically connected with the controller.
Claims (6)
1. Culture system suitable for microorganism directed evolution, including culture apparatus, culture apparatus includes fermentation cylinder, its characterized in that: the culture system also comprises a strain preservation device, wherein the strain preservation device comprises a pipe frame, a refrigerating mechanism, a pipe cover opening mechanism and a pipe body liquid injection mechanism,
The pipe rack is provided with more than 2 pipe holes for holding the preservation pipes;
the refrigerating mechanism is used for refrigerating the preservation pipes placed on the pipe rack;
The tube cover opening mechanism is used for opening and closing the tube cover of the preservation tube;
the pipe body liquid injection mechanism is used for conveying the bacterial liquid in the fermentation tank into a preservation pipe;
The fermentation tank, the refrigerating mechanism, the pipe cover opening mechanism and the pipe body liquid injection mechanism are respectively and electrically connected with the controller;
the pipe frame is of an annular structure, pipe holes are uniformly and alternately distributed on the pipe frame, and the pipe frame is connected with the pipe frame driving unit and rotates around the axis under the action of the pipe frame driving unit;
The pipe cover opening mechanism comprises a pipe cover push rod, a pipe cover lifting arm, a pipe cover motor and a pipe cover clamp, wherein the pipe cover push rod is arranged on one side of the bracket, the output end of the pipe cover push rod is connected with one end of the pipe cover lifting arm and is used for driving the pipe cover lifting arm to do lifting movement, the other end of the pipe cover lifting arm is vertically matched with a pair of guide posts arranged on the bracket, and the pipe cover motor is arranged on the pipe cover lifting arm and is used for driving the pipe cover clamp to do rotary movement in a horizontal plane;
The pipe body liquid injection mechanism comprises a liquid injection pipeline, a valve, a delivery pump, a liquid push rod, a liquid injection motor, a liquid injection lifting arm and a liquid injection head, wherein the liquid injection pipeline is communicated with the fermentation tank and a liquid joint on the liquid injection head, the liquid injection pipeline is connected with the valve and the delivery pump, the liquid injection push rod is arranged on the other side of the bracket, the output end of the liquid injection push rod is connected with the liquid injection motor and is used for driving the liquid injection motor to do lifting motion, the output end of the liquid injection motor is connected with one end of the liquid injection lifting arm and is used for driving the liquid injection lifting arm to do rotary motion in a horizontal plane, and the other end of the liquid injection lifting arm is connected with the liquid injection head;
The refrigerating mechanism comprises a refrigerating sheet and a cold transfer plate which are of annular structures, the refrigerating sheet, the cold transfer plate and the support plate of the pipe rack are sequentially connected from bottom to top, side heat insulation layers are respectively arranged on the inner side and the outer side of the cold transfer plate in a surrounding mode, an upper heat insulation layer is arranged on the upper portion of the cold transfer plate, and the refrigerating sheet and the cold transfer plate are communicated with the pipe holes and penetrate through the perforations of the upper heat insulation layer.
2. The culture system suitable for directed evolution of microorganisms according to claim 1, wherein: the pipe cover clamping device is of a sleeve structure with an upper end sealing plate and a lower end open, and the side wall of the pipe cover clamping device is provided with more than 2 slots extending from bottom to top to form spring petals for clamping the pipe cover; the inner side wall of the pipe cover clamp is provided with anti-slip rubber.
3. The culture system suitable for directed evolution of microorganisms according to claim 1, wherein: the culture system further comprises a tube fixing mechanism, wherein the tube fixing mechanism is used for fixing the tube body of the preservation tube.
4. A culture system suitable for directed evolution of microorganisms according to claim 3, characterized in that: the pipe body fixing mechanism comprises a left clamping flap, a right clamping flap and a power source, wherein the left clamping flap and the right clamping flap are respectively provided with an arc-shaped groove matched with the pipe body, the left clamping flap and the right clamping flap move in opposite directions under the action of the power source to clamp the pipe body, and the power source is an air cylinder.
5. The culture system suitable for directed evolution of microorganisms according to claim 1, wherein: the liquid adding head is sleeved with a sealing ring, the liquid adding head is further provided with a gas connector, the gas connector is connected with a gas source, and a filter element is fixed on the upper portion of the preservation tube.
6. The culture system suitable for directed evolution of microorganisms according to claim 1, wherein: the culture system further comprises a base and an outer cover, and the pipe rack, the moving mechanism, the refrigerating mechanism and the pipe cover opening mechanism are all arranged on the base and are covered by the outer cover.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010505026.7A CN111518674B (en) | 2020-06-05 | 2020-06-05 | Culture system suitable for directional evolution of microorganisms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010505026.7A CN111518674B (en) | 2020-06-05 | 2020-06-05 | Culture system suitable for directional evolution of microorganisms |
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| Publication Number | Publication Date |
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| CN111518674A CN111518674A (en) | 2020-08-11 |
| CN111518674B true CN111518674B (en) | 2024-06-04 |
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| CN202010505026.7A Active CN111518674B (en) | 2020-06-05 | 2020-06-05 | Culture system suitable for directional evolution of microorganisms |
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| CN114907964B (en) * | 2022-06-23 | 2023-06-09 | 北京蓝晶微生物科技有限公司 | Sampling system and sampling method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201962288U (en) * | 2011-03-15 | 2011-09-07 | 河南科技学院 | Strain culturing preservation pipe |
| CN110951612A (en) * | 2019-12-12 | 2020-04-03 | 中国科学院深圳先进技术研究院 | Automatic liquid changing device |
| CN212894668U (en) * | 2020-06-05 | 2021-04-06 | 江南大学 | Culture system suitable for directed evolution of microorganisms |
-
2020
- 2020-06-05 CN CN202010505026.7A patent/CN111518674B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201962288U (en) * | 2011-03-15 | 2011-09-07 | 河南科技学院 | Strain culturing preservation pipe |
| CN110951612A (en) * | 2019-12-12 | 2020-04-03 | 中国科学院深圳先进技术研究院 | Automatic liquid changing device |
| CN212894668U (en) * | 2020-06-05 | 2021-04-06 | 江南大学 | Culture system suitable for directed evolution of microorganisms |
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