CN216838008U - Cell preparation system - Google Patents
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- CN216838008U CN216838008U CN202122965199.XU CN202122965199U CN216838008U CN 216838008 U CN216838008 U CN 216838008U CN 202122965199 U CN202122965199 U CN 202122965199U CN 216838008 U CN216838008 U CN 216838008U
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Abstract
The utility model discloses a cell preparation system, comprising: a sterile operating chamber for providing a sterile environment for the cell extraction operation; a sterile transfer chamber for transferring reagents and materials for testing from a sterile environment into a sterile, aseptic processing chamber; the full-automatic cell liquid changing device is used for automatically changing liquid for the cell culture medium, and the sterile operation cabin is arranged between the sterile transfer cabin and the full-automatic cell liquid changing device; the cell culture box is used for culturing cells, and the full-automatic cell liquid changing device is arranged between the sterile operation cabin and the cell culture box. The utility model provides a cell preparation system, wherein the whole cell preparation process is completely finished in an isolator, and simple and repeated liquid changing work is automatically replaced, so that full-automatic liquid changing is realized in the whole liquid changing process, and the operation errors and the pollution risks caused by manual operation are reduced.
Description
Technical Field
The utility model relates to the technical field of cell liquid changing equipment, in particular to a cell preparation system.
Background
At present, cell preparation operation is mostly carried out in an open cell laboratory, manual operation is mainly used, and cell preparation personnel need to be trained. The quality of cell preparation is affected by the problems of pollution risk, poor reproducibility and the like caused by manual operation. If the automation can be used for replacing the manual work in the cell liquid changing process, the cell liquid changing pollution risk is reduced, and the liquid changing efficiency is greatly improved. Therefore, a cell preparation system is needed, the whole cell preparation process is completely completed in the isolator, the GMP requirement is met, the full-automatic liquid change is realized in the whole liquid change process, and the operation error and the pollution risk caused by manual operation are reduced; the liquid suction needle head can be automatically replaced, so that the automation degree is further improved, and cross contamination is avoided; simultaneously, make the imbibition syringe needle absorb the waste liquid totally through the structure setting.
China patent application publication No. CN106367343B, published as 09.21.2018, entitled fully-automatic intelligent cell culture device and control method thereof, discloses a fully-automatic intelligent cell culture device and control method thereof, which comprises a controller, a first adjusting mechanism, a second adjusting mechanism, a third adjusting mechanism, a culture rack, a first control door, a second control door, a first liquid-transferring mechanism, a second liquid-transferring mechanism, a waste liquid collecting mechanism, a bottle opening and closing mechanism and a liquid-adding mechanism, wherein at least three cavities are arranged in a storage cavity, and comprise a storage cavity, a transition cavity and a preheating cavity. But the technical scheme that this patent adopted is different with the technical scheme that this application will apply for, and it is not high to trade liquid efficiency, and the unable automatic change of imbibition syringe needle in this patent causes cross contamination easily, influences cell culture's quality, also does not set up the more thorough structure of messenger's imbibition, leads to having the incomplete problem of imbibition.
Disclosure of Invention
In order to overcome the problems caused by manual operation in the cell sterile preparation process in the prior art, the utility model provides a cell preparation system, the whole cell preparation process is completely finished in an isolator, and simple and repeated liquid changing work is replaced automatically, so that the whole liquid changing process is fully-automatically changed, and the operation errors and the pollution risks caused by manual operation are reduced.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a cell preparation system, comprising:
a sterile operating compartment for providing a sterile environment for the cell extraction operation;
a sterile transfer chamber for transferring reagents and materials for testing from a sterile environment into a sterile, aseptic processing chamber;
the full-automatic cell liquid changing device is used for automatically changing liquid for the cell culture medium, and the sterile operation cabin is arranged between the sterile transfer cabin and the full-automatic cell liquid changing device;
the cell culture box is used for culturing cells, and the full-automatic cell liquid changing device is arranged between the sterile operation cabin and the cell culture box.
In the technical scheme, the sterile transfer cabin, the sterile operation cabin, the full-automatic cell liquid changing device and the cell culture box are communicated in sequence. The process of cell sterile preparation is: 1. Inoculation: opening the sterile transfer cabin, putting peripheral blood into the sterile transfer cabin for sterilization transfer, carrying out cell extraction operation after a blood sample is transferred into the sterile operation cabin, then inoculating cells into a culture dish, manually putting the inoculated culture dish into a transfer port of the full-automatic cell liquid changing device, and transferring the culture dish into a culture box by a culture dish transfer manipulator; 2. liquid changing: the incubator door is automatically opened, the transfer manipulator takes out the culture dish and places the culture dish to an automatic liquid changing station for automatic liquid changing, then the transfer manipulator transfers the culture dish to an imaging station for photographing, and finally the transfer manipulator places the culture dish back into the incubator; 3. receiving goods: the incubator door is opened automatically, transports the manipulator and takes out the culture dish and puts to the transfer port of full-automatic cell liquid changing device and aseptic technique cabin, then the manual work is got the culture dish and is carried out the manual collection. The whole cell preparation process is completed in the isolator, and the GMP requirement is met. Simple and repeated liquid changing work is replaced by automation, and complicated single inoculation and collection work is operated by people with rich experience. Environmental parameters, centrifugal parameters, culture parameters and liquid change parameters of the whole preparation process are recorded and traceable in the whole process, and the system is provided with a computer control system and can be implanted into a standardized operation program.
Preferably, an automatic mechanical arm is arranged in the sterile operation cabin. The automatic mechanical arm can execute the automatic program of cell extraction operation to automatically extract cells.
Preferably, an embedded refrigerator, a waste channel and an embedded centrifuge are arranged in the sterile operation cabin.
Preferably, a planktonic bacteria sampler and a particle sampler are arranged in the sterile operating cabin.
Preferably, a rotating main shaft is arranged in the cell culture box, a plurality of fixing frames are circumferentially arranged on the rotating main shaft, a plurality of placing frames are arranged on the fixing frames in the vertical direction, and the rotating main shaft is driven to rotate by a driving piece. The cell culture box can move the culture dish to the position near the outlet according to the requirement, so that the operation of a transfer manipulator is facilitated.
Preferably, the fully automatic cell liquid changing device comprises:
fixing the platform;
the transfer manipulator is used for the culture dish body and is arranged on the fixed platform;
the liquid changing mechanism is arranged on the fixed platform and comprises a needle head module and a moving driving mechanism used for moving the needle head module, the needle head module comprises a fixed block, a liquid adding needle tube and a liquid absorbing needle tube, the liquid adding needle tube and the liquid absorbing needle tube are fixed on the fixed block, and the liquid absorbing needle tube is provided with a liquid absorbing needle;
The culture dish workbench is used for placing the culture dish body.
In the technical scheme, the liquid suction needle tube is communicated with an external liquid suction device and can suck waste liquid; the liquid feeding needle tube is communicated with an external cell culture solution conveying mechanism, and can provide required culture solution. The working process of the technical scheme is as follows: can take out the culture dish body in the incubator through transporting the manipulator to place the culture dish body on the culture dish workstation, stretch into the culture dish body through liquid changing mechanism with the imbibition syringe needle and carry out the imbibition, the imbibition is accomplished the back, stretches into culture dish body with the liquid feeding needle tubing and adds the culture solution, then sends the culture dish body back to the incubator through transporting the manipulator. The whole liquid changing process in the technical scheme realizes full-automatic liquid changing, and reduces operation errors and pollution risks caused by manual operation.
Preferably, the culture dish workstation is fixed with fixed platform including the fixing base and the rotation seat that set up side by side, rotates the seat and rotates with fixed platform and be connected, rotates the axis of rotation and the horizontal plane parallel arrangement of seat, and fixed platform is last to be equipped with the drive and rotates a pivoted rotation driver, and during the liquid change, the culture dish body is placed on rotating the seat.
Among the above-mentioned technical scheme, after the transfer manipulator takes out the culture dish body in with the incubator, place on the fixing base, then the culture dish plectrum can move the culture dish body to on rotating the seat, the rethread rotates the driver and makes and rotate the seat and rotate the slope, make the slope of culture dish body, the imbibition syringe needle of being convenient for absorbs the waste liquid totally, then rethread liquid feeding needle tube carries out the liquid feeding, the liquid feeding is accomplished the back, it makes and rotates the seat and rotate the repayment, the culture dish plectrum can be with on the culture dish body moves the fixing base, can send the culture dish body back to the incubator through the transfer manipulator.
Preferably, the rotating seat is eccentrically arranged below the rotating seat, the rotating driver comprises an electromagnet, the electromagnet is fixed below the rotating seat, the rotating shaft is arranged between the gravity center of the rotating seat and the electromagnet, a magnetic suction block is arranged at the position, corresponding to the electromagnet, of the lower end of the rotating seat, and when the electromagnet is electrified, the electromagnet is in contact with the magnetic suction block. The structure can realize the rotation of the rotating seat, the rotating structure is simple and reliable, the cost is low, the control is simple, and the rotation can be realized only by one electromagnet. And the rotating seat is kept horizontal by the gravity of the rotating seat.
Preferably, the lower end of the liquid adding needle tube is provided with a liquid adding needle head, and the lower end of the liquid adding needle head is bent towards the side with lower height after inclining towards the rotating seat. The structure can ensure that the liquid outlet of the liquid feeding needle is close to the culture medium and is positioned on the side wall which is relatively below the culture medium, so that the cell culture solution slowly flows down along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast.
Preferably, a rotary damper is provided on the rotating shaft. The structure can make the seat that rotates have certain buffering when rotating, avoids rotating too fast and leads to the culture solution to rock and spill.
Preferably, the fixed platform is provided with a vacuum chuck and a chuck lifting mechanism, the chuck lifting mechanism is fixed with the fixed platform, the vacuum chuck is fixed with the chuck lifting mechanism, and the vacuum chuck is arranged right above the fixed seat. The structure can be used to suck the lid on the culture dish body.
Preferably, the movement driving mechanism comprises an X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, the needle module is fixed on the Z-axis moving mechanism, the Z-axis moving mechanism is fixed on the Y-axis moving mechanism, and the Y-axis moving mechanism is fixed on the X-axis moving mechanism. The X-axis moving mechanism, the Y-axis moving mechanism and the Z-axis moving mechanism can be cylinders, oil cylinders, push rod motors, motor-driven lead screw and nut mechanisms, motor-driven gear and rack mechanisms and the like, and the motor-driven lead screw and nut mechanisms are selected. The structure can realize the translation of the movement driving mechanism in the three directions of the X axis, the Y axis and the Z axis, thereby driving the needle head module to move as required.
Preferably, a needle placing frame is arranged on the fixing platform, and a plurality of unused liquid suction needles are arranged on the needle placing frame. When a plurality of hole sites are arranged on the culture dish body, in order to avoid mutual pollution among the hole sites, after waste liquid in each hole site is absorbed, the imbibition needle head needs to be replaced, and the needle head placing frame is used for placing the unused imbibition needle head. And positioning columns are arranged around the needle head placing frame and are fixed with the fixing platform.
Preferably, a positioning column is arranged around the needle head placing frame and is fixed with the fixing platform.
Preferably, a waste liquid discharge barrel is arranged on the fixed platform. The waste liquid discharge barrel can be used for collecting redundant liquid in the liquid adding needle tube.
Preferably, a positioning column is arranged around the waste liquid discharge barrel and is fixed with the fixing platform. The reference column can be fixed a position and fix waste liquid discharge barrel.
Preferably, a microscope photographing station is arranged on the fixed platform, and a photographing fixing seat matched with the culture dish body is fixed on the microscope photographing station.
The utility model has the beneficial effects that: (1) the whole liquid changing process realizes full-automatic liquid changing, and reduces the risk of pollution caused by manual operation; (2) the liquid suction needle head can be automatically replaced; (3) the liquid outlet of the liquid feeding needle head is close to the side wall of one side of the culture medium, so that the cell culture solution slowly flows downwards along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast; (4) the rotating seat is rotated and inclined through the rotating driver, so that the culture dish body is inclined, and the liquid suction needle head can conveniently suck the waste liquid completely; (5) the rotating structure of the rotating seat is simple and reliable, the cost is low, the control is simple, the rotation can be realized only by one electromagnet, and the rotating seat is kept horizontal by utilizing the gravity of the rotating seat.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic structural view of example 2;
FIG. 3 is a schematic view of the transfer robot of the present invention;
FIG. 4 is a schematic view of a partial structure of the present invention;
FIG. 5 is a schematic view of the construction of the needle module of the present invention;
FIG. 6 is a schematic view showing the structure of a petri dish table in the utility model;
fig. 7 is a schematic view showing the construction of the waste needle collecting box of the present invention.
In the figure: a fixed platform 1, a transfer manipulator 2, a translation mechanism 2.1, a lifting mechanism 2.2, a rotating mechanism 2.3, a transfer plate 2.4, a liquid changing mechanism 3, a needle module 3.1, a fixed block 3.1.1, a liquid adding needle tube 3.1.2, a liquid absorbing needle tube 3.1.3, a clamping ring 3.1.4, a liquid absorbing needle 3.1.5, a limiting ring 3.1.6, a liquid adding needle 3.1.7, a mobile driving mechanism 3.2, a culture dish worktable 4, a fixed seat 4.1, a rotating seat 4.2, a rotating shaft 4.3, a holding tank 4.4, a rotating driver 4.5, a magnetic absorption block 4.6, a limiting block 4.7, a culture dish body 5, a needle placing frame 6, a waste needle collecting box 7, a needle removing block 8, a limiting groove 8.1, a culture dish shifting sheet 9, an adjusting base 10, an adjusting rod 11, a waste liquid waste discharging barrel 12, a positioning column 13, a microscope station 14, a sterile operation cabin 15, a refrigerator 15.1, an embedded waste channel 15.2, an embedded type waste conveying device 15.6, a floating bacteria-free particle sampler 15.6, a centrifugal machine 16, a floating bacteria particle-free sampler 15.4, a centrifugal machine, a floating bacteria cabin 16, a liquid sampler, Cell culture case 17, rotation main shaft 17.1, mount 17.2, full-automatic cell trades liquid device 18.
Detailed Description
The utility model is further described with reference to the following figures and specific embodiments.
Example 1:
as shown in fig. 1, a cell preparation system includes: a sterile operation cabin 15, a sterile transfer cabin 16, a full-automatic cell liquid changing device 18 and a cell culture box 17; the sterile operation cabin 15 is used for providing a sterile environment for cell extraction operation, and an embedded refrigerator 15.1, a waste channel 15.2, an embedded centrifuge 15.3, a planktonic bacteria sampler 15.4 and a particle sampler 15.6 are arranged in the sterile operation cabin 15; the sterile transfer chamber 16 is used for transferring the test reagent and materials from a sterile environment to the sterile operation chamber 15; the full-automatic cell liquid changing device 18 is used for automatically changing liquid for cell culture media, and the sterile operation cabin 15 is arranged between the sterile transfer cabin 16 and the full-automatic cell liquid changing device 18; cell culture case 17 is used for cultivateing the cell, and full-automatic cell trades liquid device 18 and sets up between aseptic manipulation cabin 15 and cell culture case 17, be equipped with in the cell culture case 17 and rotate main shaft 17.1, rotate main shaft 17.1 circumference and be equipped with a plurality of mount 17.2, mount 17.2 is equipped with a plurality of rack in the upper and lower direction, and driving piece drive rotates main shaft 17.1 and rotates.
In the technical scheme, the sterile transfer cabin 16, the sterile operation cabin 15, the full-automatic cell liquid changing device 18 and the cell culture box 17 are communicated in sequence. The process of cell sterile preparation is: 1. Inoculation: opening the sterile transfer cabin, putting peripheral blood into the sterile transfer cabin for sterilization transfer, carrying out cell extraction operation after a blood sample is transferred into the sterile operation cabin 15, then inoculating cells into a culture dish, manually putting the inoculated culture dish into a transfer port of a full-automatic cell liquid changing device 18, and transferring the culture dish into a culture box by a culture dish transferring manipulator 2; 2. liquid changing: the incubator door is automatically opened, the transfer manipulator 2 takes out the culture dish and puts the culture dish to an automatic liquid changing station for automatic liquid changing, then the transfer manipulator 2 transfers the culture dish to an imaging station for photographing, and finally the transfer manipulator 2 puts the culture dish back into the incubator; 3. receiving goods: the incubator door is opened automatically, and the transfer manipulator 2 takes out the culture dish and puts to the transfer port of full-automatic cell liquid changing device 18 and aseptic technique cabin 15, then the manual work is got the culture dish and is carried out the manual collection. The whole cell preparation process is completed in the isolator, and the GMP requirement is met. Simple and repeated liquid changing work is replaced by automation, and complicated single inoculation and collection work is operated by people with rich experience. Environmental parameters, centrifugal parameters, culture parameters and liquid change parameters of the whole preparation process are recorded and traceable in the whole process, and the system is provided with a computer control system and can be implanted into a standardized operation program.
Example 2:
as shown in fig. 2, based on embodiment 1, the fully automatic cell liquid changing device 18 includes a fixed platform 1, a transferring manipulator 2, a liquid changing mechanism 3, a culture dish workbench 4, and a needle placing frame 6; the transfer manipulator 2 is used for the culture dish body 5, and the transfer manipulator 2 is arranged on the fixed platform 1; as shown in fig. 3, the transferring manipulator 2 includes a translation mechanism 2.1, an elevating mechanism 2.2, a rotating mechanism 2.3 and a transferring plate 2.4, the transferring plate 2.4 is fixed on the rotating mechanism 2.3, the rotating mechanism 2.3 is arranged on the elevating mechanism 2.2, the elevating mechanism 2.2 is fixed on the translation mechanism 2.1, the translation mechanism 2.1 and the elevating mechanism 2.2 are gear rack mechanisms driven by a motor, and the rotating mechanism 2.3 is a motor.
As shown in fig. 2 and 4, the liquid changing mechanism 3 is arranged on the fixed platform 1, the liquid changing mechanism 3 comprises a needle module 3.1 and a moving driving mechanism 3.2 for moving the needle module 3.1, as shown in fig. 5, the needle module 3.1 comprises a fixed block 3.1.1, a liquid adding needle tube 3.1.2 and a liquid absorbing needle tube 3.1.3, the liquid adding needle tube 3.1.2 and the liquid absorbing needle tube 3.1.3 are fixed on the fixed block 3.1.1, and the liquid absorbing needle tube 3.1.3 is provided with a liquid absorbing needle 3.1.5; the movement driving mechanism 3.2 comprises an X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, the needle head module 3.1 is fixed on the Z-axis moving mechanism, the Z-axis moving mechanism is fixed on the Y-axis moving mechanism, and the Y-axis moving mechanism is fixed on the X-axis moving mechanism. The X-axis moving mechanism, the Y-axis moving mechanism and the Z-axis moving mechanism adopt a screw rod and nut mechanism driven by a motor. The culture dish worktable 4 is used for placing a culture dish body 5. A culture dish shifting sheet 9 is fixed on the fixed block 3.1.1, and the culture dish shifting sheet 9 is used for transversely shifting the culture dish body 5 on the culture dish workbench 4. The fixed platform 1 is provided with a waste liquid discharge barrel 12, a microscope photographing station 14, a vacuum sucker and a sucker lifting mechanism 2.2. A positioning column 13 is arranged around the waste liquid discharge barrel 12, and the positioning column 13 is fixed with the fixed platform 1. A photographing fixing seat 4.1 matched with the culture dish body 5 is fixed on the microscope photographing station 14. The sucker lifting mechanism 2.2 is fixed with the fixed platform 1, the vacuum sucker is fixed with the sucker lifting mechanism 2.2, and the vacuum sucker is arranged right above the fixed seat 4.1.
In the technical scheme, the liquid suction needle tube 3.1.3 is communicated with an external liquid suction device and can suck waste liquid; the liquid adding needle tube 3.1.2 is communicated with an external cell culture solution conveying mechanism and can provide required culture solution. The working process of the technical scheme is as follows: can take out culture dish body 5 in the incubator through transporting manipulator 2 to place culture dish body 5 on culture dish workstation 4, stretch into culture dish body 5 through liquid changing mechanism 3 with imbibition syringe needle 3.1.5 and carry out the imbibition, the back is accomplished to the imbibition, stretches into culture dish body 5 with liquid feeding needle tubing 3.1.2 and adds the culture solution, then sends culture dish body 5 back to the incubator through transporting manipulator 2. The whole liquid changing process in the technical scheme realizes full-automatic liquid changing, and reduces operation errors and pollution risks caused by manual operation.
Example 3:
as shown in fig. 2 and 4, on the basis of embodiment 2, a waste needle collecting box 7 is arranged on the fixing platform 1, a needle removing block 8 is fixed above the waste needle collecting box 7, and a plurality of unused liquid suction needles 3.1.5 are arranged in order. As shown in fig. 7, the needle releasing block 8 is provided with a limit groove 8.1 adapted to the liquid suction needle head 3.1.5, a positioning column 13 is arranged around the waste liquid discharging barrel 12, and the positioning column 13 is fixed with the fixing platform 1. The outer side wall of the liquid suction needle tube 3.1.3 is provided with a snap ring 3.1.4, and the liquid suction needle tube 3.1.3 is clamped with a liquid suction needle head 3.1.5 on the liquid suction needle tube 3.1.3 through the snap ring 3.1.4. Fixed platform 1 is last to be equipped with abandonment syringe needle and to collect box 7, and 7 tops of abandonment syringe needle are collected the box are fixed with and take off needle piece 8, take off and be equipped with the spacing groove 8.1 with imbibition syringe needle 3.1.5 adaptation on the needle piece 8. The upper end of the imbibition needle head 3.1.5 is provided with a limit ring 3.1.6, and the outer diameter of the limit ring 3.1.6 is larger than the width of the limit groove 8.1. A positioning column 13 is arranged around the waste liquid discharge barrel 12, and the positioning column 13 is fixed with the fixed platform 1.
Several unused pipette tips 3.1.5 are arranged in order. When a plurality of hole sites are arranged on the culture dish body 5, in order to avoid mutual pollution among the hole sites, after waste liquid in each hole site is absorbed, the liquid absorption needle head 3.1.5 needs to be replaced, and the needle head placing frame 6 is used for placing the unused liquid absorption needle head 3.1.5. The imbibition syringe needle 3.1.5 itself has certain elasticity, can block imbibition syringe needle 3.1.5 through snap ring 3.1.4. The technical scheme can be used for making imbibition syringe needle 3.1.5 break away from imbibition needle tubing 3.1.3, and imbibition syringe needle 3.1.5 inhales behind the waste liquid, need change, moves actuating mechanism 3.2 and moves imbibition syringe needle 3.1.5 to spacing groove 8.1 below, then upwards lifts syringe needle module 3.1, makes and takes off needle piece 8 and block the upper end of imbibition syringe needle 3.1.5, makes imbibition syringe needle 3.1.5 break away from imbibition needle tubing 3.1.3, falls into in the discarded syringe needle collection box 7. The positioning column 13 can position and fix the waste needle collecting box 7.
Example 4:
as shown in fig. 6, on the basis of embodiment 2, culture dish workstation 4 includes fixing base 4.1 and rotation seat 4.2 that set up side by side, and fixing base 4.1 is fixed with fixed platform 1, rotates seat 4.2 and is connected with fixed platform 1 rotation, rotates 4.2's axis of rotation 4.3 and horizontal plane parallel arrangement, is equipped with the drive on the fixed platform 1 and rotates 4.2 pivoted rotation driver 4.5, and during the liquid change, culture dish body 5 is placed on rotating seat 4.2. The rotating shaft 4.3 of the rotating seat 4.2 is eccentrically arranged below the rotating seat 4.2, and a rotating damper is arranged on the rotating shaft 4.3. The rotary driver 4.5 comprises an electromagnet, the electromagnet is fixed below the rotary seat 4.2, the rotary shaft 4.3 is arranged between the gravity center of the rotary seat 4.2 and the electromagnet, the magnetic attraction block 4.6 is arranged on the position of the lower end of the rotary seat 4.2 corresponding to the electromagnet, and when the electromagnet is electrified, the electromagnet is in contact with the magnetic attraction block 4.6. An adjusting base 10 is arranged below the rotating base 4.2, the adjusting base 10 is fixed with the fixed platform 1, an adjusting rod 11 which is in threaded connection with the adjusting base 10 and can be adjusted up and down is arranged on the adjusting base 10, the top end of the adjusting rod 11 is in contact with the bottom end of the rotating base 4.2, and the adjusting rod 11 and the electromagnet are arranged at two opposite ends of the rotating shaft 4.3.
Fixing base 4.1 is equipped with the holding tank 4.4 that is used for holding transport plate 2.4 of transporting manipulator 2. One end of the rotating seat 4.2, which is far away from the fixed seat 4.1, is provided with a limiting block 4.7 for limiting the position of the culture dish body 5. The lower end of the liquid adding needle tube 3.1.2 is provided with a liquid adding needle head 3.1.7, and the lower end of the liquid adding needle head 3.1.7 is bent towards the lower side of the rotating seat 4.2 after being inclined.
Among the above-mentioned technical scheme, after transfer manipulator 2 takes out culture dish body 5 in with the incubator, place on fixing base 4.1, then culture dish plectrum 9 can remove culture dish body 5 to rotating on seat 4.2, rethread rotates driver 4.5 and makes and rotate seat 4.2 and rotate the slope, make culture dish body 5 slope, the imbibition syringe needle 3.1.5 of being convenient for absorbs the waste liquid totally, then the liquid feeding is carried out to rethread liquid feeding needle tubing 3.1.2, the liquid feeding is accomplished the back, it makes and rotates seat 4.2 and rotates the level of returning to rotate 4.5 to rotate the driver, culture dish plectrum 9 can move culture dish body 5 to on the fixing base 4.1, can send culture dish body 5 back to the incubator through transferring manipulator 2. The structure can realize the rotation of the rotating seat 4.2, the rotating structure is simple and reliable, the cost is low, the control is simple, and the rotation can be realized only by one electromagnet. And the rotating seat 4.2 is kept horizontal by the gravity of the rotating seat 4.2.
The beneficial effects of the utility model are: (1) the whole liquid changing process realizes full-automatic liquid changing, and reduces operation errors and pollution risks caused by manual operation; (2) the liquid suction needle head 3.1.5 can be automatically replaced; (3) the liquid outlet of the liquid adding needle 3.1.7 is close to the side wall of one side of the culture medium, so that the cell culture solution slowly flows down along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast; (4) the rotating seat 4.2 is rotated and inclined by rotating the driver 4.5, so that the culture dish body 5 is inclined, and the liquid absorption needle 3.1.5 can absorb the waste liquid completely; (5) the rotating structure of the rotating seat 4.2 is simple and reliable, the cost is low, the control is simple, the rotation can be realized only by one electromagnet, and the rotating seat 4.2 is kept horizontal by the gravity of the rotating seat 4.2.
Claims (9)
1. A cell preparation system, comprising:
a sterile operating compartment for providing a sterile environment for the cell extraction operation;
a sterile transfer chamber for transferring reagents and materials for testing from a sterile environment into a sterile, aseptic processing chamber;
the full-automatic cell liquid changing device is used for automatically changing liquid for the cell culture medium, and the sterile operation cabin is arranged between the sterile transfer cabin and the full-automatic cell liquid changing device;
The cell culture box is used for culturing cells, and the full-automatic cell liquid changing device is arranged between the sterile operation cabin and the cell culture box.
2. The system for preparing cells according to claim 1, wherein a robot arm is provided in the aseptic manipulation chamber.
3. The cell preparation system according to claim 1 or 2, wherein the fully automatic cell changing apparatus comprises:
a fixed platform;
the transfer manipulator is used for the culture dish body and is arranged on the fixed platform;
the liquid changing mechanism is arranged on the fixed platform and comprises a needle head module and a moving driving mechanism used for moving the needle head module, the needle head module comprises a fixed block, a liquid adding needle tube and a liquid absorbing needle tube, the liquid adding needle tube and the liquid absorbing needle tube are fixed on the fixed block, and the liquid absorbing needle tube is provided with a liquid absorbing needle;
the culture dish worktable is used for placing the culture dish body.
4. The system according to claim 3, wherein the petri dish table comprises a fixed base and a rotating base arranged side by side, the fixed base is fixed to the fixed platform, the rotating base is rotatably connected to the fixed platform, a rotating shaft of the rotating base is parallel to a horizontal plane, a rotating driver for driving the rotating base to rotate is arranged on the fixed platform, and the petri dish body is placed on the rotating base during liquid change.
5. The cell preparation system according to claim 3, wherein the movement driving mechanism comprises an X-axis moving mechanism, a Y-axis moving mechanism, and a Z-axis moving mechanism, the needle module is fixed to the Z-axis moving mechanism, the Z-axis moving mechanism is fixed to the Y-axis moving mechanism, and the Y-axis moving mechanism is fixed to the X-axis moving mechanism.
6. The cell preparation system according to claim 3, wherein a needle rack is provided on the fixing platform, and a plurality of unused pipetting needles are provided on the needle rack.
7. The system of claim 3, wherein the fixed platform is provided with a waste liquid discharge barrel.
8. The cell preparation system according to claim 7, wherein a positioning column is disposed around the waste liquid discharge barrel, and the positioning column is fixed to the fixing platform.
9. The cell preparation system according to claim 3, wherein a microscope photographing station is provided on the fixing platform, and a photographing fixing seat adapted to the culture dish body is fixed on the microscope photographing station.
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| CN202122965199.XU CN216838008U (en) | 2021-11-22 | 2021-11-22 | Cell preparation system |
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| CN202122965199.XU CN216838008U (en) | 2021-11-22 | 2021-11-22 | Cell preparation system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117511735A (en) * | 2023-11-09 | 2024-02-06 | 厦门眼科中心有限公司 | Biological cell culture device |
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2021
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117511735A (en) * | 2023-11-09 | 2024-02-06 | 厦门眼科中心有限公司 | Biological cell culture device |
| CN117511735B (en) * | 2023-11-09 | 2024-05-17 | 厦门眼科中心有限公司 | Biological cell culture device |
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