CN114891634A

CN114891634A - Automatic cell culture equipment

Info

Publication number: CN114891634A
Application number: CN202210543965.XA
Authority: CN
Inventors: 刘鹏飞; 李磊; 李恒宇
Original assignee: Shanghai Daojian Mechanical & Electrical Technology Co ltd; Wenzhou Research Institute Of Shanghai University
Current assignee: Shanghai Daojian Mechanical & Electrical Technology Co ltd; Wenzhou Research Institute Of Shanghai University
Priority date: 2022-05-18
Filing date: 2022-05-18
Publication date: 2022-08-12

Abstract

The invention relates to automatic cell culture equipment which comprises an outer frame rack structure, an automatic operating room and an automatic culture room, wherein the automatic operating room and the automatic culture room are arranged inside the outer frame rack structure, and the automatic operating room comprises a first outer frame metal plate fixedly arranged inside the outer frame rack structure. This automatic cell culture equipment, through under the effect at blake bottle stack mechanism, make blake bottle group shift to blake bottle group moving mechanism, then in moving to automatic culture room through blake bottle group moving mechanism, after blake bottle group moves to automatic operation room, blake bottle screw cap mechanism work, open the bottle lid of blake bottle, then get through pipettor suction head and put the module, realize getting the suction head automatically, automatic butt joint reagent pipe and automatic butt joint blake bottle, then supply corresponding reagent through reagent supply module in to the blake bottle, after the supply is accomplished, blake bottle screw cap mechanism can be twisted on the bottle lid again.

Description

Automatic cell culture equipment

Technical Field

The invention relates to the technical field of cell culture, in particular to automatic cell culture equipment.

Background

Cell culture refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to enable the cells to survive, grow, reproduce and maintain main structure and function, and is also called cell cloning technology, the formal term in biology is cell culture technology, and cell culture is an essential process for the whole bioengineering technology and one of the biological cloning technologies, the cell culture is large-scale cloning of cells, the cell culture technology can be used for making a large amount of cells into simple single cells or rarely differentiated multiple cells through mass culture, which is an essential link of the cloning technology, and the cell culture is cloning of the cells.

The cell culture technology is an important and common technology in a cell biology research method, a large number of cells can be obtained through cell culture, and signal transduction, cell anabolism, cell growth and proliferation and the like of the cells can be researched by the cell culture technology.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides automatic cell culture equipment which has the advantages of automation, no humanization, high efficiency and the like, and solves the problems of low efficiency, high cost, low flux and poor safety of manual operation in the conventional cell culture process.

In order to achieve the purpose, the invention provides the following technical scheme: an automatic cell culture device comprises an outer frame structure, an automatic operation room and an automatic culture room, wherein the automatic operation room and the automatic culture room are arranged inside the outer frame structure;

the pipette tip taking and placing module comprises a first front-and-back moving module, a left-and-right moving module, a first up-and-down moving module, a tip taking and placing clamping jaw, a liquid absorption auxiliary clamping jaw and a tip removing mechanism, wherein the left-and-right moving module is arranged in a first outer frame metal plate, the left-and-right moving module is provided with the first front-and-down moving module, the first front-and-down moving module can move the first front-and-back moving module, the first front-and-down moving module can move the first up-and-down moving module, the first up-and-down moving module is provided with the tip taking and placing clamping jaw, the first up-and-down moving module can move the tip taking and placing clamping jaw up and down, the liquid absorption auxiliary clamping jaw is arranged in the first outer frame metal plate, and the tip removing mechanism is arranged in the first outer frame metal plate;

the culture bottle cap screwing mechanism comprises a mounting frame, a second front-and-back moving module, a moving mechanism, a rotating module, a clamping jaw and a clamping head, wherein the mounting frame is fixedly mounted in a first outer frame metal plate, the second front-and-back moving module is arranged on the mounting frame, the moving mechanism is arranged at the top of the mounting frame, the rotating module is arranged on the moving mechanism, the rotating module is fixedly connected with the second front-and-back moving module, the rotating module can enable the moving mechanism to move, the clamping jaw is fixedly mounted at an output shaft of the rotating module, and the clamping head is fixedly mounted on a driving rod of the clamping jaw;

the culture bottle group moving mechanism comprises a left horizontal moving module, a right horizontal moving module, a bottle group supporting plate, a bottle group fixing plate and a culture bottle, the left horizontal moving module and the right horizontal moving module are arranged in a first outer frame metal plate, the bottle group supporting plate is arranged on the left horizontal moving module and the right horizontal moving module, the left horizontal moving module and the right horizontal moving module can enable the bottle group supporting plate to move, the bottle group fixing plate is movably arranged at the top of the bottle group supporting plate, and the culture bottle is fixedly arranged on the bottle group fixing plate.

Further, frame rack construction includes first shell sheet metal framework, fixed mounting has the electrical apparatus box in the first shell sheet metal framework, first shell sheet metal framework's surface articulates there is the door between automatic operation, first shell sheet metal framework's surface articulates there is the door between the automatic culture that is located door one side between automatic operation, first shell sheet metal framework's bottom is equipped with between lower floor's waste recovery, first shell sheet metal framework's fixed surface installs always controls the touch-sensitive screen.

Further, door between automatic operation room and automatic cultivation room door constitute by outside stainless steel isolation door and inboard glass sealing door, swing joint has the recovery box in the lower floor's waste recovery room.

Further, the inner chamber side surface top fixed mounting of first outer frame panel beating has a first ultraviolet disinfection lamp, the inner chamber bottom fixed mounting of first outer frame panel beating has the pipettor suction head box, be equipped with reagent in the first outer frame panel beating and supply with the module.

Further, the reagent supply module includes reagent heat preservation box and peristaltic pump, the bottom of reagent heat preservation box is equipped with the refrigeration module, reagent storage jar has been placed in the reagent heat preservation box, the peristaltic pump has the connecting pipe with reagent storage jar intercommunication.

Further, including second shell sheet metal frame between the automatic culture, fixed mounting has the second ultraviolet disinfection lamp in the second shell sheet metal frame, be equipped with the blake bottle in the second shell sheet metal frame and stack the mechanism, fixed mounting has the microscope in the second shell sheet metal frame.

Further, the second shell sheet metal frame comprises a shell sheet metal, wherein a CO automatic supply system is arranged in the shell sheet metal, a temperature keeping system is arranged in the shell sheet metal, and a waste collecting box is connected to the bottom of the shell sheet metal in a sliding mode.

Further, the automatic CO supply system comprises a CO gas cylinder, a gas pump and a CO sensor, and the temperature maintaining system comprises a heating wire, a fan and a temperature sensor.

Further, the culture bottle stacking mechanism comprises a culture bottle rack, a second up-and-down moving module and a guide rod, the culture bottle rack is fixedly installed in a second shell metal plate frame, a culture bottle group inductor is fixedly installed on the culture bottle rack, the second up-and-down moving module is arranged in the second shell metal plate frame, a culture bottle group positioning plate sliding on the surface of the guide rod is arranged on the surface of the second up-and-down moving module, the second up-and-down moving module can enable the culture bottle group positioning plate to move up and down, and a third back-and-forth moving module is fixedly installed on the surface of the culture bottle group positioning plate.

Furthermore, the third front-back moving module is an electric screw rod, an output shaft of the third front-back moving module penetrates through the positioning plate of the culture bottle group, and a push plate is fixedly mounted at the other end of the output shaft of the third front-back moving module.

Compared with the prior art, the technical scheme of the application has the following beneficial effects:

the automatic cell culture equipment transfers a culture bottle group to a culture bottle group moving mechanism under the action of a culture bottle stacking mechanism, then moves to an automatic culture room through the culture bottle group moving mechanism, after the culture bottle group moves to the automatic operation room, the culture bottle cap screwing mechanism works to open the bottle cap of the culture bottle, then the automatic suction head taking, the automatic reagent tube butt joint and the automatic culture bottle butt joint are realized through the pipette suction head taking and placing module, then the corresponding reagent is supplied into the culture bottle through the reagent supply module, after the supply is finished, the bottle cap can be screwed up again by the culture bottle cap screwing mechanism, and the waste suction head can be dropped off by the pipette suction head taking and placing module and dropped into the lower waste recovery room, therefore, the automatic culture of the cells is realized, the manual operation is not needed, and the purposes of automation, no humanization and high efficiency are realized.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic structural diagram of an outer frame rack structure according to the present invention;

FIG. 3 is a cross-sectional view of the automated process chamber of the present invention;

FIG. 4 is a cross-sectional view of the automated handler of the present invention;

FIG. 5 is a schematic diagram of the structure of the reagent supply module according to the present invention;

fig. 6 is a schematic structural view of a pipette tip picking and placing module according to the present invention;

FIG. 7 is a schematic structural view of a culture bottle screw-capping mechanism according to the present invention;

FIG. 8 is a schematic structural view of a moving mechanism of a culture flask set according to the present invention;

FIG. 9 is a partial cross-sectional view of an automatic culture chamber according to the present invention;

FIG. 10 is a partial sectional view of an automatic culture chamber according to the present invention;

FIG. 11 is a schematic view showing the structure of a culture bottle stacking mechanism according to the present invention.

In the figure: 1. an outer frame structure; 11. a first shell sheet metal frame; 12. an electrical box; 13. automatically operating the compartment door; 14. an automatic culture room door; 15. a lower waste recovery room; 16. a master control touch screen; 2. an automatic operation room; 21. a first outer frame metal plate; 22. a first ultraviolet disinfection lamp; 23. a pipette tip cartridge; 24. a reagent supply module; 241. a refrigeration module; 242. a reagent heat preservation box; 243. a reagent storage tank; 244. a peristaltic pump; 245. a connecting pipe; 25. a pipette tip pick-and-place module; 251. a first back-and-forth movement module; 252. a left-right moving module; 253. a first up-down moving module; 254. a sucker picking and placing clamping jaw; 255. liquid suction auxiliary clamping jaws; 256. a suction head removing mechanism; 26. a culture bottle cap screwing mechanism; 261. a mounting frame; 262. a second back-and-forth movement module; 263. a moving mechanism; 264. a rotation module; 265. a clamping jaw; 266. a chuck; 27. a culture bottle group moving mechanism; 271. a left and right horizontal movement module; 272. a bottle group supporting plate; 273. a bottle group fixing plate; 274. a culture bottle; 3. an automatic culture room; 31. a second housing sheet metal frame; 311. a shell metal plate; 312. CO2 auto feed system; 313. a temperature maintenance system; 314. a waste collection box; 32. a second ultraviolet disinfection lamp; 33. a culture bottle stacking mechanism; 331. a culture bottle material rack; 332. a culture flask group sensor; 333. a second up-down moving module; 334. a third forward-backward movement module; 335. a guide bar; 336. a positioning plate of the culture bottle group; 34. and (4) a microscope.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Referring to fig. 1, the automatic cell culture apparatus of the present embodiment includes an outer frame structure 1, an automatic operating room 2 and an automatic culture room 3, wherein the automatic operating room 2 and the automatic culture room 3 are disposed inside the outer frame structure 1, and the automatic operating room 2 and the automatic culture room 3 are connected to each other.

Please refer to fig. 2, the outer frame rack structure 1 includes a first shell sheet metal frame 11, an electrical box 12 is fixedly installed in the first shell sheet metal frame 11, the electrical box 12 contains a control electrical device and a filtration vortex fan, an automatic operation room door 13 is hinged on the surface of the first shell sheet metal frame 11, an automatic cultivation room door 14 located on one side of the automatic operation room door 13 is hinged on the surface of the first shell sheet metal frame 11, a lower waste recovery room 15 is arranged at the bottom of the first shell sheet metal frame 11, a recovery box is movably connected in the lower waste recovery room 15, a waste suction head is stored through the recovery box, a general control touch screen 16 is fixedly installed on the surface of the first shell sheet metal frame 11, and the operation of the whole device is controlled under the effect of the general control touch screen 16.

Under the action of the automatic operation room door 13 and the automatic cultivation room door 14, the automatic operation room 2 and the automatic cultivation room 3 are controlled to be opened and closed, and the automatic operation room door 13 and the automatic cultivation room door 14 are both composed of an outer stainless steel isolation door and an inner glass sealing door.

Referring to fig. 3-4, the automatic operating room 2 includes a first outer frame metal plate 21 fixedly mounted inside the outer frame structure 1, a pipette tip picking and placing module 25, a culture bottle capping mechanism 26 and a culture bottle group moving mechanism 27 are disposed in the first outer frame metal plate 21, and under the action of the pipette tip picking and placing module 25, operations such as automatic picking and placing of a pipette tip, automatic docking of a reagent tube, automatic docking of a culture bottle and automatic dropping of a pipette tip are realized, and under the action of the culture bottle capping mechanism 26, automatic opening and closing of a culture bottle cap are realized, the culture bottle group moving mechanism 27 is disposed in the automatic operating room 2 and the automatic culturing room 3, and under the action of the culture bottle group moving mechanism 27, the culture bottle group is moved between the automatic operating room 2 and the automatic culturing room 3.

And the bottom of the first outer frame metal plate 21 is provided with a through hole which is communicated with the lower waste recovery room 15, and under the action of the through hole, the waste suction head enters the lower waste recovery room 15 and finally falls into the recovery box.

The utility model discloses a sterilization device, including first outer frame panel beating 21, inner chamber side surface top fixed mounting has first ultraviolet disinfection lamp 22, disinfect to the inside of first outer frame panel beating 21 through shining of first ultraviolet disinfection lamp 22, the inner chamber bottom fixed mounting of first outer frame panel beating 21 has pipettor pipette tip box 23, pipettor pipette tip box 23 is used for placing the pipettor tip, be equipped with reagent supply module 24 in the first outer frame panel beating 21, under the effect of reagent supply module 24, realize the supply of medicament.

It should be noted that the device needs to be self-uv sterilized for 3 days before cell culture, so as to ensure a sterile environment inside the device.

Referring to fig. 5-6, the reagent supply module 24 includes a reagent heat preservation box 242 and a peristaltic pump 244, a refrigeration module 241 is disposed at the bottom of the reagent heat preservation box 242, the reagent heat preservation box 242 is kept at a certain temperature under the action of the refrigeration module 241, so as to ensure the storage of the reagent, a reagent storage tank 243 is disposed in the reagent heat preservation box 242, the reagent storage tank 243 is used for the storage of the reagent, the peristaltic pump 244 is communicated with the reagent storage tank 243 to form a connection pipe 245, and the reagent in the reagent storage tank 243 is pumped out under the action of the peristaltic pump 244.

Referring to fig. 6, the pipette tip picking and placing module 25 includes a left and right moving module 252, a liquid absorbing auxiliary clamping jaw 255 and a tip removing mechanism 256, which are fixedly installed in the first outer frame metal plate 21, the tip removing mechanism 256 is located at an opening at the bottom of the first outer frame metal plate 21, so that when a tip falls off under the action of the tip removing mechanism 256, the tip enters the lower waste recycling chamber 15 through the opening, the left and right moving module 252 is fixedly installed with a first front and back moving module 251, the first front and back moving module 251 is fixedly installed with a first up and down moving module 253, the first up and down moving module 253 is fixedly installed with a tip picking and placing clamping jaw 254, and the angle of the tip picking and placing clamping jaw 254 is adjustable.

The left-right moving module 252 and the first front-back moving module 251 are both composed of slide rails and slide tables, and slide on the slide rails through the slide tables, thereby realizing left-right moving and front-back moving.

Under the action of the left-right moving module 252, the first front-back moving module 251 and the first up-down moving module 253, the front-back, left-right and up-down movement of the suction head taking and placing clamping jaws 254 are realized, so that the suction head taking and placing clamping jaws 254 move to the pipette suction head box 23, and the suction head clamping, the reagent tube butt joint and the culture bottle butt joint are carried out.

Referring to fig. 7, the culture bottle cap screwing mechanism 26 includes four mounting frames 261 fixedly mounted in the first outer frame metal plate 21, each mounting frame 261 includes four support rods and four mounting plates, a second front-and-back movement module 262 is fixedly mounted on a side surface of each mounting frame 261, a moving mechanism 263 is fixedly mounted at the top of each mounting frame 261, a rotating module 264 fixedly connected with the second front-and-back movement module 262 is fixedly connected to a surface of each moving mechanism 263, a clamping jaw 265 is fixedly mounted at an output shaft of each rotating module 264, two clamping heads 266 are fixedly mounted on a driving rod of each clamping jaw 265, and clamping of a culture bottle cap is realized under the action of the two clamping heads 266, and meanwhile, under the action of the rotating module 264, the clamping jaws 26 are driven to rotate, so that the clamping heads 265 are driven to rotate, and automatic opening and closing of the culture bottle cap are realized.

When it should be noted that, the moving mechanism 263 and the second front-and-back moving module 262 both include a sliding rail and a sliding table sliding on the sliding rail, wherein the sliding table is fixed to the rotating module 264, so that when the second front-and-back moving module 262 moves, the rotating module 264 is driven to move back and forth.

Referring to fig. 8, the culture bottle group moving mechanism 27 includes a left and right horizontal moving module 271 fixedly mounted in the first outer frame metal plate 21, the left and right horizontal moving module 271 includes a slide rail at the bottom and a sliding table sliding on the slide rail, and two ends of the slide rail are respectively located in the automatic operating room 2 and the automatic culturing room 3, so that the sliding table can slide between the automatic operating room 2 and the automatic culturing room 3, a bottle group supporting plate 272 is fixedly mounted at the top of the left and right horizontal moving module 271, a bottle group fixing plate 273 is movably mounted at the top of the bottle group supporting plate 272, the bottle group fixing plate 273 is a plate body provided with grooves, the number of the grooves is several, and the grooves are adapted to the size of the culture bottles, culture bottles 274 are fixedly mounted on the bottle group fixing plate 273, and the number of the culture bottles 274 corresponds to the number of the grooves on the surface of the bottle group fixing plate 273 one to one.

The transfer of the culture bottles between the automated working room 2 and the automated culture room 3 is realized by the culture bottle group transfer mechanism 27.

Referring to fig. 9, the automatic culture room 3 includes a second housing sheet metal frame 31, a second ultraviolet disinfection lamp 32 is fixedly installed in the second housing sheet metal frame 31, the inside of the second housing sheet metal frame 31 is disinfected by the irradiation of the second ultraviolet disinfection lamp 32, a culture bottle stacking mechanism 33 is installed in the second housing sheet metal frame 31, the culture bottle stacking mechanism 33 is used for loading and taking out a culture bottle, a microscope 34 is fixedly installed in the second housing sheet metal frame 31, and the culture condition of cells in the culture bottle is observed through the microscope 34.

Referring to fig. 10, the second housing sheet metal frame 31 includes a housing sheet metal 311, an automatic CO2 supply system 312 is disposed in the housing sheet metal 311, the automatic CO2 supply system 312 is used for increasing CO2, a temperature maintaining system 313 is disposed in the housing sheet metal 311, the temperature maintaining system 313 ensures stable temperature inside, a waste collecting box 314 is slidably connected to the bottom of the housing sheet metal 311, and waste is collected by the waste collecting box 314.

The CO2 automatic supply system 312 consists of a CO2 gas cylinder, an air pump and a CO2 sensor, the temperature maintenance system 313 consists of a heating wire, a fan and a temperature sensor, and under the action of the CO2 automatic supply system 312 and the temperature maintenance system 313, the stability of the environment is ensured during cell culture.

Referring to fig. 11, the culture bottle stacking mechanism 33 includes a culture bottle rack 331 fixedly mounted in the second housing sheet metal frame 31, a second up-down moving module 333 and a guide rod 335, the culture bottle rack 331 is used for placing a culture bottle, a culture bottle group sensor 332 is fixedly mounted on the culture bottle rack 331, a culture bottle group positioning plate 336 sliding on the guide rod 335 is fixedly mounted on the surface of the second up-down moving module 333, and a third up-down moving module 334 is fixedly mounted on the surface of the culture bottle group positioning plate 336.

The second up-down moving module 333 includes a slide rail and a slide block sliding on the slide rail, and the slide block is fixedly connected to the positioning plate 336 for culturing bottle group, and the slide rail is vertically disposed, so that the slide block slides on the slide rail to make the positioning plate 336 for culturing bottle group slide up and down.

The third back-and-forth moving module 334 is an electric screw, an output shaft of the third back-and-forth moving module 334 penetrates through the positioning plate 336 of the culture bottle group, the other end of the output shaft of the third back-and-forth moving module 334 is fixedly provided with a connecting plate, and the connecting plate is driven to be connected with the bottle group fixing plate 273 under the action of the third back-and-forth moving module 334, so that the bottle group fixing plate 273 is moved back to the culture bottle rack 331 or is taken down from the culture bottle rack 331.

It should be noted that the third forward-backward moving module 334 may also be a driving device such as a hydraulic push rod capable of performing linear reciprocating motion.

The working principle of the above embodiment is as follows:

during operation, the left and right horizontal moving module 271 works and moves into the automatic culture room 3, at this time, the second up-down moving module 333 drives the culture bottle group positioning plate 336 to move up and down, the culture bottle group sensor 332 corresponds to the culture bottle group, the third back-and-forth moving module 334 moves the bottle group fixing plate 273 onto the bottle group supporting plate 272, the left and right horizontal moving module 271 moves the culture bottle group into the automatic operation room 2, after the culture bottle group moves into the automatic operation room 2, the clamp claw 265 in the culture bottle cap screwing mechanism 26 clamps the culture bottle cap through the clamp head 266, the rotary module 264 rotates the clamp claw 265 to automatically open the culture bottle cap, and the first back-and-forth moving module 251 in the pipette head module 25 moves, Under the action of the left-right moving module 252 and the first up-down moving module 253, the sucker taking and placing clamping jaws 254 are driven to move, automatic sucker taking, automatic reagent tube butt joint and automatic butt joint of culture bottles are realized under the action of the plurality of liquid suction auxiliary clamping jaws 255 and the sucker removing mechanism 256, then corresponding reagents are injected into the corresponding culture bottles through the reagent supply module 24, after the reagent injection is completed, the culture bottle caps are closed through the culture bottle capping mechanism 26, and meanwhile, the used suckers finally fall into the lower-layer waste recovery room 15 under the action of the sucker removing mechanism 256, so that the automatic culture of cells is realized, manual operation is not needed, and the purposes of automation, humanization and high efficiency are realized.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a cell automatic culture equipment, includes that frame rack construction (1), automatic operation room (2) and automatic culture room (3), its characterized in that: the automatic operating room (2) and the automatic culturing room (3) are arranged inside the outer frame rack structure (1), the automatic operating room (2) comprises a first outer frame metal plate (21) fixedly arranged inside the outer frame rack structure (1), and a pipette tip taking and placing module (25), a culture bottle screwing and covering mechanism (26) and a culture bottle group moving mechanism (27) are arranged in the first outer frame metal plate (21);

the pipette tip taking and placing module (25) comprises a first front-and-back moving module (251), a left-and-right moving module (252), a first up-and-down moving module (253), a tip taking and placing clamping jaw (254), a liquid absorption auxiliary clamping jaw (255) and a tip removing mechanism (256), wherein the left-and-right moving module (252) is arranged in a first outer frame metal plate (21), the first front-and-back moving module (251) is arranged on the left-and-right moving module (252), the first front-and-back moving module (251) can move the first front-and-back moving module (251), the first up-and-down moving module (251) can move the first up-and-down moving module (253), the tip taking and placing clamping jaw (254) is arranged on the first up-and-down moving module (253), and the tip taking and placing clamping jaw (254) can move up and down by the first up-and-down moving module (253), the liquid suction auxiliary clamping jaw (255) is arranged in the first outer frame sheet metal (21), and the sucker removing mechanism (256) is arranged in the first outer frame sheet metal (21);

the culture bottle cap screwing mechanism (26) comprises an installation frame (261), a second front-and-back moving module (262), a moving mechanism (263), a rotating module (264), a clamping jaw (265) and a clamping head (266), wherein the installation frame (261) is fixedly installed in a first outer frame metal plate (21), the second front-and-back moving module (262) is arranged on the installation frame (261), the moving mechanism (263) is arranged at the top of the installation frame (261), the rotating module (264) is arranged on the moving mechanism (263), the rotating module (264) is fixedly connected with the second front-and-back moving module (262), the rotating module (264) can enable the moving mechanism (263) to move, the clamping jaw (265) is fixedly installed at an output shaft of the rotating module (264), and the clamping head (266) is fixedly installed on a driving rod of the clamping jaw (265);

culture flask group moving mechanism (27) including controlling horizontal migration module (271), bottle group layer board (272), bottle group fixed plate (273) and culture flask (274), horizontal migration module (271) sets up in first outer frame panel beating (21) about, bottle group layer board (272) set up on controlling horizontal migration module (271), control horizontal migration module (271) and can make bottle group layer board (272) remove, the top movable mounting of bottle group layer board (272) has bottle group fixed plate (273), fixed mounting has culture flask (274) on bottle group fixed plate (273).

2. An automatic cell culture apparatus according to claim 1, wherein: frame rack construction (1) includes first shell sheet metal frame (11), fixed mounting has electrical apparatus box (12) in first shell sheet metal frame (11), the surface of first shell sheet metal frame (11) articulates there is door (13) between automatic operation, the surface of first shell sheet metal frame (11) articulates there is door (14) between the automatic cultivation that is located automatic operation room door (13) one side, the bottom of first shell sheet metal frame (11) is equipped with between lower floor's waste recovery (15), the fixed surface of first shell sheet metal frame (11) installs total control touch-sensitive screen (16).

3. An automatic cell culture apparatus according to claim 2, wherein: the automatic operation room door (13) and the automatic cultivation room door (14) are composed of an outer stainless steel isolation door and an inner glass sealing door, and a recovery box is movably connected in the lower waste recovery room (15).

4. An automatic cell culture apparatus according to claim 1, wherein: the utility model discloses a kit, including first outer frame panel beating (21), inner chamber side surface top fixed mounting has first ultraviolet disinfection lamp (22), the inner chamber bottom fixed mounting of first outer frame panel beating (21) has pipettor suction head box (23), be equipped with reagent supply module (24) in first outer frame panel beating (21).

5. An automatic cell culture apparatus according to claim 4, wherein: reagent supply module (24) include reagent heat preservation box (242) and peristaltic pump (244), the bottom of reagent heat preservation box (242) is equipped with refrigeration module (241), reagent storage jar (243) have been placed in reagent heat preservation box (242), peristaltic pump (244) and reagent storage jar (243) intercommunication have connecting pipe (245).

6. An automatic cell culture apparatus according to claim 1, wherein: automatic cultivate room (3) including second shell sheet metal frame (31), fixed mounting has second ultraviolet disinfection lamp (32) in second shell sheet metal frame (31), it stacks mechanism (33) to be equipped with the blake bottle in second shell sheet metal frame (31), fixed mounting has microscope (34) in second shell sheet metal frame (31).

7. An automatic cell culture apparatus according to claim 6, wherein: the second shell sheet metal frame (31) comprises a shell sheet metal (311), a CO2 automatic supply system (312) is arranged in the shell sheet metal (311), a temperature keeping system (313) is arranged in the shell sheet metal (311), and a waste collection box (314) is connected to the bottom of the shell sheet metal (311) in a sliding mode.

8. An automatic cell culture apparatus according to claim 7, wherein: the CO2 automatic supply system (312) consists of a CO2 gas cylinder, a gas pump and a CO2 sensor, and the temperature maintaining system (313) consists of a heating wire, a fan and a temperature sensor.

9. An automatic cell culture apparatus according to claim 6, wherein: the culture bottle stacking mechanism (33) comprises a culture bottle rack (331), a second up-down moving module (333) and a guide rod (335), wherein the culture bottle rack (331) is fixedly installed in a second shell metal plate frame (31), a culture bottle group inductor (332) is fixedly installed on the culture bottle rack (331), the second down-moving module (333) is arranged in the second shell metal plate frame (31), the surface of the second up-down moving module (333) is provided with a culture bottle group positioning plate (336) sliding on the surface of the guide rod (335), the second up-down moving module (333) can enable the culture bottle group positioning plate (336) to move up and down, and the surface of the culture bottle group positioning plate (336) is fixedly provided with a third module (334) moving back and forth.

10. An automatic cell culture apparatus according to claim 9, wherein: the third front-back moving module (334) is an electric screw rod, an output shaft of the third front-back moving module (334) penetrates through the positioning plate (336) of the culture bottle group, and a push plate is fixedly mounted at the other end of the output shaft of the third front-back moving module (334).