CN113528341B

CN113528341B - Biological tissue production system and production method

Info

Publication number: CN113528341B
Application number: CN202110621002.2A
Authority: CN
Inventors: 赖雪聪; 倪孝杰; 徐铭恩
Original assignee: Regenovo Biotechnology Co ltd
Current assignee: Regenovo Biotechnology Co ltd
Priority date: 2021-06-03
Filing date: 2021-06-03
Publication date: 2024-05-14
Anticipated expiration: 2041-06-03
Also published as: CN113528341A

Abstract

The invention relates to a biological tissue production system and a production method, wherein the system comprises at least one culture operation module, at least one culture module, at least one program cooling module and at least one consumable management module; all modules can be mechanically connected through a fixed interface and electrically connected through a control interface; the culture operation module is used for simulating manual operation and can realize automation of cell culture; the culture module is used for culturing cells and has the functions of high-temperature sterilization and automatic transportation; the program cooling module performs program cooling and low-temperature storage on a sample to be frozen; the consumable management module provides various required consumables for the operation of the biological tissue production system. According to the technical scheme, freezing or culture can be carried out according to the state of cell output, dependence on the operation level and experience of personnel in production is reduced, the number of each module can be freely combined according to the needs, and the method has extremely strong flexibility and expansibility, and meets the complex cell production requirements.

Description

Biological tissue production system and production method

Technical Field

The invention relates to the field of biological tissue production, in particular to a biological tissue production system and a biological tissue production method.

Background

The cell expansion is completed by purely manual operation or semi-automatic operation by means of equipment, the operation level and operation experience of operators are needed to be relied on for manual production, the efficiency is low, and meanwhile, the cell expansion mode is limited due to the fact that the production architecture mode is single, and increasingly complex cell production requirements are difficult to meet.

Disclosure of Invention

The invention aims to overcome the defects that the efficiency is low, the artificial production is dependent and the complex cell production requirement is difficult to meet in the prior art, thereby providing a biological tissue production system.

In a first aspect, the present invention provides a biological tissue production system, including at least one culture operation module, at least one culture module, at least one program cooling module, and at least one consumable management module; all modules can be mechanically connected through a fixed interface and electrically connected through a control interface; the culture operation module is used for simulating manual operation and can realize automation of cell culture; the culture module is used for culturing cells and has the functions of high-temperature sterilization and automatic transportation; the program cooling module performs program cooling and low-temperature storage on a sample to be frozen; the consumable management module provides various required consumables for the operation of the biological tissue production system.

Further, the culture operation module comprises a culture storage mechanism for inoculating cell strains, a thawing and uncapping device for thawing and uncapping the frozen culture storage mechanism, a consumable storage device provided with a centrifuge tube, a clamping and moving device for clamping the centrifuge tube and/or the culture storage mechanism, and a liquid treatment device for treating and moving reagents; the clamping and moving device comprises a batch grabbing mechanism, a clamping mechanism and a liquid moving mechanism, wherein the batch grabbing mechanism can grab the culture storage mechanisms such as the freezing storage assembly and the like, the clamping mechanism can operate the culture storage mechanisms such as the culture bottle, the centrifuge tube and the like, and the liquid moving mechanism can be used for liquid sucking or liquid discharging and the like; the thawing cover opening device is matched with the batch grabbing mechanism to finish thawing operation of the freezing and storing assembly.

Further, the culture module comprises a culture box body, a rotary culture disc at least partially arranged in the culture box body, an automatic door on the culture box body and a manipulator outside the automatic door; the culture box body is provided with a culture cavity and a first accommodating cavity which is arranged at intervals with the culture cavity, the sensor assembly is placed in the first accommodating cavity, the culture cavity is communicated with the first accommodating cavity through the first air duct and the second air duct, and when the culture cavity is sterilized at high temperature, the sensor assembly can be protected, and the environmental state in the culture cavity can be monitored in real time; the manipulator cooperates with the rotatory dish of cultivateing, realizes cultivateing the automatic transportation of storage mechanism to through the isolation effect of automatically-controlled door, the protection the manipulator is prevented from cultivateing the injury that the cavity high temperature sterilization brought.

Further, the program cooling module comprises a program cooling mechanism and an ultralow temperature storage mechanism, wherein the program cooling mechanism is used for cooling the frozen sample; the program cooling mechanism realizes ordered step cooling by transferring between any two adjacent constant-temperature freezing blocks through the circulating mechanism and the temperature guide blocks relative to the circulating motion of each constant-temperature freezing block, and can flexibly add a new sample to be frozen into a freezing well of the temperature guide block while carrying out step cooling, or can be placed in the temperature guide block with corresponding set temperature according to the initial temperature of the newly added sample to be frozen; the ultralow temperature storage mechanism is used for preserving frozen samples cooled by the program cooling mechanism for a long time.

Further, the consumable management module comprises a carrier for setting consumable materials and a clamping assembly for transferring the consumable materials; the carrier can be of rotary or static design, and can be driven by a motor when rotating, and in the rotating process, the residual space on the supporting table is locked for mounting the carrier, so that the space utilization rate is improved; when a type of consumable is required to be prepared, the first sensor is used for identifying the in-place installation condition of the carrier, the identification piece on the clamping assembly is used for identifying the consumable, and the clamping assembly is used for transferring the consumable to the target position after confirmation.

Further, the biological tissue production system further comprises a shell and an environment control mechanism, wherein the modules are arranged in the shell in a one-to-one correspondence manner, and the environment control mechanism is embedded and arranged in the modules; the environment control mechanism comprises a regulating box arranged at the top of the shell, a first primary filter arranged in the regulating box and communicated with the outside, a first centrifugal fan arranged in the regulating box, a first efficient filter communicated with the inside of the shell, a first sensor group arranged on the regulating box, a first temperature and humidity controller for performing constant temperature and humidity treatment on filtered air, and a first sterilization actuator capable of rapidly generating ozone gas or hydrogen oxide steam so as to ensure the required clean and aseptic environment state in each module.

Further, the biological tissue production system further comprises a control module, wherein the control module is mechanically connected with the modules through a fixed interface and is electrically connected with the modules through the control interface; the control module comprises a positioning column, an electric control cabinet fixed on the positioning column, a torsion shaft rotatably arranged on the positioning column and a control panel fixedly connected with the torsion shaft; the electric control cabinet is used for controlling the operation of the biological tissue production system, and the control panel is an interface for user interaction.

Further, the biological tissue production system further comprises an external environment control module, wherein the external environment control module comprises a fan housing, a partition plate, a second primary filter, a second centrifugal fan, a second temperature and humidity controller, a second sterilization executor, a second sensor group, an air supply pipeline and a second efficient filter; the air pressure bin is arranged above the air pressure bin, the air inlet bin is arranged below the air pressure bin, the second primary filter is vertically arranged on the side surface of the air pressure bin, the second sensor group is positioned at the position of the pressure cavity leading to the air supply pipeline and is used for monitoring the environment in the biological tissue production system and feeding back the environment to the external environment control module in real time to ensure the accuracy of the internal environment control of the biological tissue production system, the air supply pipeline is connected with the pressure cavity and each module, clean high-pressure air flow processed by the external environment control module is conveyed to each module through the air supply pipeline, the second efficient filter is positioned at the tail end and the top of each module reached by the air supply pipeline, and the air supply pipeline can be filtered to form vertical downward parallel air flow meeting the requirement of cleanliness.

In a second aspect, the present invention also provides a method for producing a biological tissue production system, for use in the biological tissue production system, comprising the steps of:

according to the cell culture conditions in the culture storage mechanism, at least one culture operation module, at least one culture module, at least one program cooling module, at least one consumable management module and at least one control module are mutually connected through respective fixed interfaces and control interfaces to form a complete biological tissue production system, and an external environment control module can be additionally arranged according to requirements.

And after the sterilization is completed, the temperature and humidity control of the environment control mechanism is started, the cleanliness stability in the equipment is ensured, and the environment control function can be executed by the external environment control module.

And consumable materials used in the consumable material management module, such as a culture bottle, a centrifuge tube, a freezing tube, a pipetting gun head box and the like, are sprayed or pasted with two-dimension codes/one-dimension codes, so that the consumable materials can be conveniently identified, and the operation logic and the operation program of the biological tissue production system are set in the operation module according to the cell types required to be cultured and the expected output cell quantity.

The cell strain used for the culture may be a cell in an attached culture flask, a frozen cell stored in a freezing tube, or a cell stored in an apparatus; the cells enter the inside of the biological tissue production system from the outside of the biological tissue production system through the culture operation module; transferring the cell sap in the culture storage mechanism into a centrifuge tube for centrifugal treatment to obtain centrifugal cell sap; pouring the supernatant of the centrifugal cell sap, and filling the reagent into the centrifuge tube for treatment to obtain a cell suspension; detecting and obtaining a density value of the cell suspension, inoculating the cell suspension into a new culture storage mechanism according to the density value, and judging a preset state of cells to be output; if the frozen cells are output in a preset mode, transferring the new culture storage mechanism to a program cooling module for cooling, transferring the new culture storage mechanism to an ultralow temperature storage mechanism for ultralow temperature preservation, and transferring the frozen cells to the outside of the biological tissue production system through a culture operation module after the preset time; if the adherent cells are preset to be output, transferring the new culture storage mechanism into a culture module for cell culture, and judging whether the growth state of the cells meets the passage requirement after the culture is performed for preset time; and if the cultured cells meet the requirement of passage, carrying out passage operation on the adherent cells by a culture operation module or transferring the adherent cells to the outside of the biological tissue production system.

Compared with the prior art, the invention has the beneficial effects that:

According to the biological tissue production system provided by the technical scheme, the at least one culture operation module, the at least one culture module, the at least one program cooling module and the at least one consumable management module are mutually connected and communicated, and the clamping and moving device, the defrosting and uncapping device and the liquid treatment device in the culture operation module can better replace manual work to perform various operations, so that the operation efficiency is improved; the culture module has a high-temperature sterilization function, can protect the sensor assembly from being damaged by high-temperature sterilization, and the manipulator and the rotary culture disc are cooperatively matched to realize automatic transfer of the culture storage mechanism, and protect the manipulator from being damaged by high-temperature sterilization of the culture cavity through the isolation function of the automatic door; the program cooling mechanism of the program cooling module and the ultralow temperature storage mechanism work together and are used for outputting frozen samples of frozen storage; be equipped with the consumptive material on the carrier of consumptive material management module, need not to prepare temporarily, when needing to allocate the consumptive material of a type, discern the installation condition in place of carrying the article by first sensor, the identification piece on the clamping assembly is right the consumptive material is discerned, will after confirming by the clamping assembly the consumptive material shifts to the target position, has improved the correct rate that the consumptive material was prepared, reduces the operating time that increases because of personnel's preparation appears the mistake. The biological tissue production system has complete automatic operation conditions and capability, can freeze or culture according to the state of cell output, can continuously supply consumable materials, ensures that each module can be better closed and independently operated, has high production efficiency, reduces the participation degree of personnel in the cell culture process, reduces the dependence on the operation level and experience of the personnel in production, can be set according to the needs, and freely combines each module according to application needs, has extremely strong flexibility and expansibility, and meets the complex cell production needs.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

FIG. 1 is a front view of a biological tissue production system according to one embodiment of the present invention;

FIG. 2 is a front view of a biological tissue production system according to another embodiment of the present invention;

FIG. 3 is a top view of a biological tissue production system according to the present invention;

FIG. 4 is a schematic view showing the internal structure of the culture operation module shown in FIG. 1;

FIG. 5 is a schematic view of a clamping mechanism and a flask according to an embodiment of the present invention;

FIG. 6 is an exploded view of a clamping mechanism, a cryoprotectant tube, and a centrifuge tube according to an embodiment of the present invention;

FIG. 7 is a schematic diagram of a batch grabbing mechanism and a freezing storage shell according to an embodiment of the present invention;

FIG. 8 is a schematic view of the batch grabbing mechanism shown in FIG. 7;

FIG. 9 is a schematic diagram of the consumable storage device of FIG. 4;

FIG. 10 is a schematic view of the pipetting mechanism of FIG. 4;

FIG. 11 is a schematic view showing a structure of a liquid treatment apparatus according to an embodiment of the present invention;

FIG. 12 is a schematic view showing the internal structure of the culture module shown in FIG. 1;

FIG. 13 is a schematic view of a portion of the assembly shown in FIG. 12;

FIG. 14 is a schematic diagram illustrating an internal structure of the program cooling module shown in FIG. 1;

FIG. 15 is a schematic view of a partial structure of the cooling mechanism of FIG. 14;

FIG. 16 is a schematic view of the cooling mechanism of FIG. 15 from another perspective;

FIG. 17 is a schematic diagram of the ultra-low temperature storage mechanism of FIG. 14;

FIG. 18 is a schematic diagram illustrating an internal structure of the consumable management module of FIG. 1;

FIG. 19 is a schematic view of a portion of the components shown in FIG. 18;

FIG. 20 is a schematic diagram of an environmental control mechanism in an embodiment of the invention;

FIG. 21 is a schematic view of the steering module shown in FIG. 2;

reference numerals illustrate:

100. A culture operation module; 10. a culture storage mechanism; 20. a cryopreservation assembly; 21. freezing and storing the shell; 22. a freezing tube; 30. a culture bottle; 41. a mechanical arm; 50. a clamping mechanism; 51. a first driving member; 52. a clamping group; 53. a clamping part; 60. a batch grabbing mechanism; 61. a base shell; 62. a grab; 63. grabbing a pipe fitting; 64. a cylinder; 70. A pipetting mechanism; 111. pipetting gun heads; 120. thawing the cover opening device; 150. a consumable storage device; 151. a carrier; 160. A liquid treatment device; 161. a pipette path; 162. a heating assembly; 163. a pump body; 164. adding a sample; 170. a refrigerator; 171. a microscope; 172. a centrifuge;

200. a culture module; 201. a sensor assembly; 202. an air duct I; 203. an air duct II; 204. a culture cavity; 205. a first accommodating cavity; 210. rotating the culture plate; 220. an automatic door; 230. a manipulator;

300. A program cooling module; 310. a program cooling mechanism; 311. constant temperature freezing blocks; 312. a temperature guiding block; 313. a cooling well; 320. an ultralow temperature storage mechanism; 321. a storage rack; 322. a lifting mechanism; 323. freezing bin; 324. a refrigerating member; 330. a circulation mechanism; 340. a transfer mechanism;

400. a consumable management module; 410. a carrier rack; 411. a support; 412. A carrier; 420. a clamping assembly; 431. A first sensor;

500. A housing;

600. An environmental control mechanism; 601. a control box; 602. a first primary filter; 603. a first centrifugal fan; 604. a first high efficiency filter; 605. a first sensor group; 606. a first temperature and humidity controller; 607. a first sterilization actuator;

700. A control module; 701. positioning columns; 702. an electric control cabinet; 703. a control panel; 704. a torsion shaft; 705. a touch screen; 706. an alarm; 707. a signal lamp; 708. an emergency stop switch;

800. An external environment control module; 801. a wind shell; 802. a partition plate; 803. a second primary filter; 804. a second centrifugal fan; 805. a second temperature and humidity controller; 806. a second sterilization actuator; 807. a second sensor group; 808. an air supply pipeline; 809. a second high-efficiency filter;

900. a control interface; 910. and fixing the interface.

Detailed Description

The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

Referring to fig. 1-21, an embodiment of the present invention provides a biological tissue production system, comprising: the functional module group comprises at least one culture operation module 100, at least one culture module 200, at least one program cooling module 300 and at least one consumable management module 400 which are mutually connected and communicated.

The culture operation module 100 is a main action part of a functional module group, various operations are simulated manually, the culture module 200 is a place for cell growth and differentiation, the program cooling module 300 reduces the temperature to a preset temperature according to a preset rate and maintains the temperature for a period of time, the consumable management module 400 is a unique window for solid consumables to enter the functional module group, is a warehouse of solid consumables of the functional module group, has complete automatic operation conditions and capability, can freeze or culture according to the output state of cells, can continuously supply consumables, enables the functional module group to perform better closed independent operation, has high production efficiency, reduces the participation of personnel in the cell culture process, reduces the dependence on personnel operation level and experience in production, can be set into a plurality of functional module groups, can be set according to needs in the number of each module in each functional module group, has extremely strong flexibility and expansibility, and meets the complex cell production requirements by freely combining each module according to application requirements.

Each module is provided with a shell 500, a fixed interface 910 and a control interface 900 are arranged on the side surface of each shell 500, each module is mechanically connected with each other through the fixed interface 910, the control interface 900 is combined with or separated from the fixed interface 910, the control interface 900 is used for electrically connecting control circuits of each module, and the fixed interface 910 can adopt modes of screw locking, pin and pin hole locking, electromagnetic lock locking, air lock locking and the like.

The culture operation module 100 comprises a culture storage mechanism for inoculating cell strains, a thawing and uncapping device 120 for thawing and uncapping the frozen culture storage mechanism, a consumable storage device 150 provided with a centrifuge tube 110, a clamping and moving device for clamping the centrifuge tube 110 and/or the culture storage mechanism, and a liquid treatment device 160 for treating and moving reagents. The culture storage mechanism can be a freezing storage component, a culture bottle, a centrifuge tube or a freezing storage sample and the like.

It should be noted that the clamping and moving device may be used to clamp the centrifuge tube 110, a culture storage mechanism, etc., the culture storage mechanism may be transferred from the outside to the inside of the casing 500 during the production process, or may be disposed inside the casing 500 before the production, the cell strain to be expanded by the culture storage mechanism may be an adherent cell inoculated to the culture flask 30, or may be a cell in a frozen state in the freezing tube 22, the new culture storage mechanism is an empty culture flask 30, and is obtained from the consumable storage device 150 by the clamping and moving device, and the access of the culture storage mechanism to the biological tissue production system may be completed by the clamping and moving device, or may be other transfer mechanisms.

It will be appreciated that, as shown in fig. 4-11, the housing 500 on which the culture operation module 100 is mounted is further provided with a mechanical arm 41, and the mechanical arm 41 and the clamping mechanism 50 cooperate to better replace manual operations to improve the operation efficiency, and use consumables such as the centrifuge tube 110 required for storing the carrier 410 to avoid the problem of terminating the operation due to consumable shortage, reduce the participation of personnel in the cell culture process, and improve the production automation.

The clamping device comprises a clamping mechanism 50 for clamping the centrifuge tube 110 and/or the culture storage mechanism, the clamping mechanism 50 comprises a first driving piece 51 and a plurality of clamping groups 52 fixed on the first driving piece 51, each clamping group 52 is clamped on the outer surface of the centrifuge tube 110 or the culture storage mechanism, each clamping group 52 comprises a plurality of clamping parts 53 which are circumferentially arranged along the side surface of the first driving piece 51, it can be understood that the clamping mechanism 50 can be used for transferring articles between the culture operation module 100 and other modules, as shown in fig. 5-6, the first driving piece 51 drives the clamping groups 52, so that the clamping parts 53 in each clamping group 52 move towards or away from each other, the opening or closing of the clamping groups 52 is realized, the number, the size, the shape and the like of the clamping groups 52 can be adjusted as required, and each clamping group 52 can clamp the centrifuge tube 110 or the culture bottle 30 or the freezing tube 22 and the like.

In one embodiment, when the cell strain to be expanded is a cell in a frozen state in the freezing tube, in order to realize the clamping and transferring of the freezing tube 22 entering the functional module group, the culture and storage mechanism comprises a freezing and storage assembly 20, the freezing and storage assembly 20 comprises a freezing and storage shell 21 and the freezing and storage tube 22 installed in the freezing and storage shell 21, the clamping and transferring device further comprises a batch grabbing mechanism 60, and the batch grabbing mechanism 60 comprises a base shell 61, a grabbing piece 62 hinged on the base shell 61, and a grabbing pipe piece 63 installed on the bottom surface of the base shell 61. It will be appreciated that one or more of the freezing pipes 22 may be stored in the freezing and storing shell 21, as shown in fig. 7-8, since the gripping member 62 is hinged to the base shell 61, the gripping member 62 may rotate relative to the base shell 61, so as to not only clamp or unclamp the freezing and storing shell 21, but also adaptively clamp the freezing and storing shells 21 with different sizes, the gripping member 62 may clamp and rotate the upper cover in the freezing and storing shell 21, so as to open the freezing and storing shell 21, and when the freezing and storing pipe 22 in the freezing and storing shell 21 needs to be transferred, the gripping pipe 63 is fixed with the freezing and storing pipe 22 in an interference fit manner under the action of the mechanical arm 41, and the number and the position of the gripping pipe 63 will be fixed on the gripping pipe 63 to transfer the freezing and storing pipe 22 to the target position.

In order to remove the frozen storage tubes 22 fixed on the grabbing tube 63, the batch grabbing mechanism 60 further includes an air cylinder 64 disposed on one side of the base shell 61 away from the grabbing tube 63, a moving member fixedly connected with an output end of the air cylinder 64 and mounted in the base shell 61, and a pushing member fixed on one side of the moving member away from the air cylinder 64 and penetrating through the grabbing tube 63, wherein the air cylinder 64 is used for driving the moving member to move up and down relative to the base shell 61.

In order to defrost the freezing tube in the freezing assembly 20, the culture operation module 100 includes a thawing cover opening device 120 for installing consumables such as a centrifuge tube 110, as shown in fig. 9-10, the consumable storage device 150 further includes a carrier 151, a pipette tip 111 on the carrier 151 for transferring liquid, and the clamping and transferring device further includes a pipetting mechanism 70, where the pipetting mechanism 70 cooperates with the pipette tip 111, and can be used for transferring liquid in a cell culture process such as transferring the cell liquid in the culture bottle 30, the freezing tube 22, the centrifuge tube 110, and the like. Under the combined action of the pipetting mechanism 70 and the pipetting gun head 111, the centrifuged cell liquid can be made into a cell suspension by repeatedly blowing the centrifuge tube 110, or the adherent cells digested by digestive enzymes in the culture flask 30 can be made into a cell suspension by repeatedly blowing the centrifuge tube 110; of course, the centrifuge tube 110 and the cells in the flask 30 can also be shaken into a cell suspension by the cooperation of the gripper mechanism 50 and the robotic arm 41.

In one embodiment, the culture storage mechanism further comprises a culture flask 30 inoculated with cells, the culture operation module 100 further comprises a liquid treatment device 160 for transferring the reagent into the culture flask 30 or the centrifuge tube 110, as shown in fig. 11, the liquid treatment device 160 comprises a pipette path 161, a heating component 162 and a pump body 163 both mounted on the pipette path 161, and a sample adding member 164 which is disposed in communication with one end of the pipette path 161, and the other end of the pipette path 161 is disposed in communication with the culture flask 30 or the centrifuge tube 110. The sample adding member 164 is a storage carrier for the liquid to be transferred, and may be a bottle with any volume and shape, the pipette path 161 is used for transferring the liquid from the sample adding member 164 to the culture bottle 30 or the channel in the centrifuge tube 110, and under the action of the pump body 163, the liquid in the sample adding member 164 is transferred to the heating component 162 through the pipette path 161, and is heated by the heating component 162 and then transferred to the culture bottle 30 or the centrifuge tube 110.

The liquid treatment apparatus 160 is used for heating the biological agent stored in the refrigerator 170 or the biological agent in other locations before entering the centrifuge tube 110 or the culture storage mechanism, the heating component 162 is used for returning the liquid to the normal temperature, and the heating means of the heating component 162 includes, but is not limited to, water bath heating, oil bath heating, metal bath heating, hot air stream heating, and the like.

In other embodiments, the culture operation module 100 may further be provided with a microscope 171 for observing cell culture conditions in real time, a centrifuge 172 for separating particles in a cell suspension, a refrigerator 170 for storing experimental reagents at low temperature, a gripper-mounted warehouse for storing gripper-moving devices, and devices for treating liquid and solid wastes.

The culture module 200 comprises a culture box body, a sensor assembly 201, an air duct I202 and an air duct II 203, wherein the culture box body is provided with a culture cavity 204 and a first accommodating cavity 205 which is arranged at intervals with the culture cavity 204, the sensor assembly 201 is arranged in the first accommodating cavity 205, the air duct I202 penetrates through the side wall of the culture box body, one end of the air duct I202 is communicated with the culture cavity 204, the other end of the air duct I202 is communicated with the first accommodating cavity 205, one end of the air duct II 203 is communicated with the first accommodating cavity 205, and the other end of the air duct II is communicated with the culture cavity 204.

It will be appreciated that, as shown in fig. 12-13, when the packaged cell liquid needs to be cultured, the culture storage mechanism is transferred into the culture module 200, the gas in the culture cavity 204 can flow into the accommodating cavity 205 via the first air duct 202, the sensor assembly 201 detects the gas in the accommodating cavity 205 to obtain corresponding information such as temperature, humidity, carbon dioxide concentration and the like in real time, and then the gas flowing into the accommodating cavity 205 flows back into the culture cavity 204 provided with the culture storage mechanism via the second air duct 203. Preferably, the sensor assembly 201 may be: the temperature sensor, the humidity sensor and the carbon dioxide sensor are respectively used for detecting the temperature, the humidity and the carbon dioxide concentration of the gas in the culture cavity 204.

In other embodiments, in order to realize the operations of moving in and out the culture bottle 30 inside the casing 500 provided with the culture module 200, the culture box body is provided with an automatic door 220 for opening and closing, and a manipulator 230 arranged in the casing 500, the manipulator 230 and the culture box body are arranged at intervals, so that the manipulator 230 moves the culture storage mechanism into the culture cavity 204 or out of the culture cavity 204, and the manipulator 230 can also be used for transferring the culture bottle 30 from the culture module 200, thereby being beneficial to transferring articles among the modules, and meanwhile, the automatic door 220 component plays a role of isolating the culture cavity 204 from the manipulator 230, and can effectively protect the manipulator 230 in the process of sterilizing the culture cavity 204 at high temperature.

In one embodiment, the program cooling module 300 includes a program cooling mechanism 310, where the program cooling mechanism 310 includes at least two constant temperature freezing blocks 311 with different temperatures, at least two temperature guiding blocks 312, and a circulation mechanism 330 for fixing each of the temperature guiding blocks 312, a cooling surface is formed on a same side of each of the constant temperature freezing blocks 311, each of the temperature guiding blocks 312 is formed with a cooling well 313 for accommodating the culture storage mechanism, and any one of the temperature guiding blocks 312 is attached to any one of the cooling surfaces and can move relatively.

It will be appreciated that, as shown in fig. 14-16, when the packaged cell liquid needs to be cooled, the culture storage mechanism is turned into the program cooling module 300, any one of the temperature guide blocks 312 can be moved from the cooling surface of one of the constant temperature freezing blocks 311 to the cooling surface of the other constant temperature freezing block 311, so that the temperature guide blocks 312 can obtain energy from the cooling surface to achieve the cooling effect, each of the temperature guide blocks 312 is fixedly connected to the circulation mechanism 330, the circulation mechanism 330 can rotate relative to the constant temperature freezing block 311, and when the circulation mechanism 330 is driven, the temperature guide blocks 312 and the circulation mechanism 330 synchronously perform the circulation motion, and one of the temperature guide blocks 312 can be moved from the cooling surface of one of the constant temperature freezing blocks 311 to the cooling surface of the adjacent constant temperature freezing block 311.

In one embodiment, the above-mentioned cooling program module 300 may realize, in addition to the step cooling by the cooling program mechanism 310, the ultra-low temperature storage of the cell suspension in the freezing tube 22 at-196 ℃ by the ultra-low temperature storage mechanism 320, where the cooling program module 300 further includes the ultra-low temperature storage mechanism 320 for long-term storage of the cell liquid in the cooling program mechanism 310, and the ultra-low temperature storage mechanism 320 includes: the storage rack 321, a lifting mechanism 322 connected to the storage rack 321, a freezing bin 323 connected with the lifting mechanism 322, and a refrigerating piece 324 fixedly arranged on the outer surface of the freezing bin 323.

It will be appreciated that, as shown in fig. 17, the storage rack 321 is used for storing the freezing storage tubes 22, the lifting mechanism 322 can drive the storage rack 321 to move, the lifting mechanism 322 can move the storage rack 321 into or out of the freezing bin 323 in the ascending or descending process, the refrigerating piece 324 can perform the effect of refrigerating the freezing bin 323, so that the internal temperature of the freezing bin 323 is always maintained at-196 ℃, and the freezing storage tubes 22 at-80 ℃ can be cooled to-196 ℃. It should be noted that, the lifting mechanism 322 may be any moving component that can be used for position transfer, such as a pneumatic cylinder, a hydraulic cylinder, an electric push rod, a conveying guide rail 441, etc., and the working principle of the refrigerating member 324 includes, but is not limited to, cooling by a semiconductor refrigerating sheet, cooling by slow evaporation and absorption of liquid nitrogen, heat exchange refrigeration by a heat engine, etc.

In other embodiments, in order to enable material to be transported within the temperature reduction program module 300 or to other modules, a transport mechanism 340 may be disposed within the housing 500 in which the temperature reduction program module 300 is disposed, the transport mechanism 340 being movable laterally or longitudinally.

The consumable management module 400 comprises a carrier 410 for setting consumable, a clamping assembly 420 for transferring the consumable, and an identification mechanism, wherein the identification mechanism comprises a first sensor 431 arranged towards the carrier 410 and used for detecting the position of the consumable set on the carrier 410, and the first sensor 431 is arranged on the clamping assembly 420 and used for identifying the identification piece of the consumable type.

It should be noted that, the carrier 410 may be provided in plurality according to the need, the carrier 410 may be provided to rotate or be stationary, and may be driven by a motor when rotating; the consumable comprises a centrifuge tube 110, a liquid-transferring gun head 111, a disposable counting plate and other articles for standby in the culture process; the identification piece can be a scanning terminal and is used for scanning a two-dimensional code or a one-dimensional code on the consumable; the clamping assembly 420 can transfer consumables from the carrier 410 to the consumable management module 400, dispense consumables to the consumable storage device 150, and transfer consumables from other modules than the consumable management module 400 to the carrier 410.

It can be appreciated that the consumable to be used is disposed in the consumable management module 400, the consumable management module 400 allocates the consumable to the consumable storage device 150, as shown in fig. 18-19, the consumable is stored by the carrier 410, and can be continuously supplied to the consumable storage device 150, the clamping component 420 and the recognition mechanism realize automatic allocation and transfer of the consumable, and the consumable management assisting device also has the function of assisting in consumable recognition management, when a type of consumable needs to be allocated, the consumable to be used is recognized from the carrier 410 by the first sensor 431 and the recognition component, and the corresponding consumable is transferred to the target position by the clamping component 420, and the consumable is not required to be temporarily allocated when needed, so that the situation of insufficient consumable is avoided, the normal operation of cell culture can be effectively ensured, the accuracy of consumable allocation is improved by the combined action of the first sensor 431, the recognition component and the clamping component 420, the operation time increased due to the occurrence of preparation errors of personnel is reduced, and the allocation time is greatly saved.

In one embodiment, the biological tissue production system includes an environmental control mechanism 600 and a plurality of housings 500, where the functional module group includes a plurality of functional modules, each of the functional modules is installed in the housing 500 in a one-to-one correspondence manner, the environmental control mechanism 600 includes a control box 601 installed at the top of the housing 500, a first primary filter 602 installed in the control box 601 and communicated with the outside, a first centrifugal fan 603 installed in the control box 601, a first efficient filter 604 communicated with the interior of the housing 500, and a first sensor group 605 installed on the control box 601. It can be understood that, as shown in fig. 20, the working environment inside each housing 500 can be regulated in real time, the air outside the housing 500 enters the regulating box 601 after being primarily filtered by the first primary filter 602, the first centrifugal fan 603 can form air flow to drive the air to flow when operating, so as to provide driving force for air circulation, and the air inside the regulating box 601 can flow into the housing 500 after being secondarily filtered by the first high-efficiency filter 604 under the driving of the first centrifugal fan 603; the arrangement of the first efficient filter 604 can effectively improve the cleanliness of the air entering the housing 500, and on the other hand, the air can form parallel air flow from top to bottom under the action of the first centrifugal fan 603.

In order to be convenient for carry out constant temperature and humidity regulation and control to the gas in the regulation and control box 601, first temperature and humidity controller 606 has been installed to this regulation and control box 601 inside, in order to carry out disinfection and sterilization to the internal environment of casing 500, this regulation and control box 601 inside has installed first sterilization executor 607, outside air can get into inside regulation and control box 601 after first primary filter 602, first temperature and humidity controller 606 carries out constant temperature and humidity processing to the air after filtering, first sterilization executor 607 can produce ozone gas or hydrogen oxide steam fast, in order to need to be right the inside air after the constant temperature and humidity processing of disinfection and sterilization of casing 500 mixes and flows to the casing 500 inside after first high-efficient filter 604 secondary filtration.

The first sensor group 605 can monitor the environmental state inside the housing 500 in real time, and the first sensor group 605 at least partially stretches into the housing 500, so that the environmental state inside the housing 500 can be measured in real time and fed back to the first temperature and humidity controller 606, so as to ensure the accuracy of the environmental control inside the housing 500. Preferably, the first sensor group 605 may be one or more of a wind speed sensor, a humidity sensor, a temperature sensor, an ozone concentration sensor, and a hydrogen peroxide concentration sensor.

In one embodiment, the biological tissue production system further includes a control module 700 electrically connected to the functional module group, the control module 700 includes a positioning column 701, an electric control cabinet 702 fixed on the positioning column, a torsion shaft 704 rotatably disposed on the positioning column 701, and a control panel 703 fixedly connected to the torsion shaft 704, as shown in fig. 21, the control module 700 may be mechanically connected to the functional module group through a fixed interface 910 disposed on the positioning column 701, the control module 700 may be electrically connected to the functional module group through the control interface 900, the electric control cabinet 702 is used for controlling the operation of the biological tissue production system, the control panel 703 is a user-interactive interface, the control panel 703 has a touch screen 705, an alarm 706, a signal lamp 707, and an emergency stop switch 708, and the torsion shaft 704 makes the control panel 703 freely rotate relative to the positioning column 701, so as to adjust a use angle.

The culture operation module 100 and the culture module 200 are controlled to operate by respective internal controllers, and the controllers in the culture operation module 100 or the culture module 200 may control other modules, or may be controlled to operate by the control module 700 in a unified manner.

In one embodiment, the biological tissue production system further includes an external environmental control module 800, as shown in fig. 1, where the external environmental control module 800 includes a wind housing 801, a partition plate 802, a second primary filter 803, a second centrifugal fan 804, a second temperature and humidity controller 805, a second sterilization actuator 806, a second sensor group 807, a wind supply duct 808, and a second efficient filter 809, and the partition plate 802 partitions the wind housing 801 into an upper wind pressure bin and a lower wind inlet bin; the second primary filter 803 is vertically installed on the side surface of the air shell 801, the second sensor group 807 is located at the position where the pressure cavity leads to the air supply pipeline 808, and is used for measuring the internal environment of the equipment and feeding back to the external environment control module 800 in real time, so as to ensure the accuracy of the internal environment control of the biological tissue production system, the air supply pipeline 808 is connected with the pressure cavity and each module, and clean high-pressure air flow processed by the external environment control module 800 is conveyed to each module through the air supply pipeline 808, the second high-efficiency filter 809 is located at the tail end and the top of each module where the air supply pipeline 808 reaches, namely, the clean high-pressure air flow conveyed to each module by the air supply pipeline 808 is finally filtered by the second high-efficiency filter 809, so that the vertical downward parallel air flow meeting the cleanliness requirement is formed.

It should be noted that, the working principles of the second centrifugal fan 804, the second primary filter 803, the second temperature and humidity controller 805, and the second sterilization actuator 806 may be referred to the above-mentioned environment control mechanism 600; the external environment control module 800 is additionally provided for the biological tissue production system, and can be used together with the culture operation module 100, the culture module 200, the program cooling module 300 and the consumable management module 400, so that all functions of the internal environment control of each module can be realized.

In summary, in the embodiment of the present invention, at least one culture operation module 100, at least one culture module 200, at least one program cooling module 300 and at least one consumable management module 400 are connected to each other through a fixed interface 910 and a control interface 900 according to the culture conditions of cells, and in addition, an external environment control module 800 can be additionally installed according to actual needs, and in cooperation with an environment control mechanism 600 in each module, the corresponding modules are sterilized, and after the sterilization is completed, the environment control mechanism 600 performs temperature and humidity regulation on the corresponding modules; or the external environment control module 800 can perform sterilization and temperature and humidity regulation operations, and operation logic and operation programs are set in the control module 700 according to the cell types required to be cultured and the expected output cell quantity, and various consumable materials required by the consumable material management module 400 are supplied in the operation process of the biological tissue production system.

The present invention also provides a method for producing a biological tissue production system, for use in the biological tissue production system, with continued reference to fig. 1-21, the method comprising the steps of:

Judging whether cells to be expanded are adherent cells in the culture flask 30 or cells in a frozen state in the freezing tube 22, if the cells to be expanded are adherent cells, judging whether the growth state of the adherent cells in the culture flask 30 meets the passage requirement by a microscope 171, if the cells to be expanded are adherent cells, performing passage operation, pouring waste culture medium in the culture flask 30 into a device for liquid waste by the clamping mechanism 50, transferring the culture flask 30 to the liquid processing device 160, filling digestive enzymes restored to normal temperature in the refrigerator 170 into the culture flask 30 by the liquid processing device 160, transferring the culture flask 30 filled with the digestive enzymes into the culture module 200 under the combined action of the clamping mechanism 50 and the manipulator 230 for a preset time, then removing a culture box, transferring the culture flask 30 to the liquid processing device 160 by the clamping mechanism 50, pouring the culture medium restored to normal temperature in the refrigerator 170 into the culture flask 30 by the liquid processing device 160, stopping the action of the digestive enzymes, and removing the digestive enzymes from the digestive system by the pipette 70 and the pipette 111 acquired on the digestion device 150, or pouring the waste into the solid waste after the cell is separated from the culture flask by the operation of the digestive system, and the cell is poured into the device.

Or transferring the cell liquid in the culture storage mechanism into the centrifuge tube 110, and transferring the centrifuge tube 110 onto a centrifuge 172 by the clamping mechanism 50 for centrifugal treatment to obtain centrifugal cell liquid; pouring the supernatant of the centrifugal cell sap into a liquid waste device, moving the centrifuge tube 110 onto the liquid treatment device 160 by the clamping mechanism 50, filling the culture medium which is restored to normal temperature by the liquid treatment device 160 in the refrigerator 170 into the centrifuge tube 110, obtaining a cell suspension in the centrifuge tube 110 through the sucking and spitting action of the pipetting mechanism 70 and the pipetting gun head 111 or shaking of the clamping mechanism 50, detecting the density value of the cell suspension, wherein the detected density value can be obtained by moving a preset amount of the cell suspension from the centrifuge tube 110 into a counter through the pipetting gun head 111 (refer to the prior art), detecting and recording the density value of the cell suspension by the counter, and pouring consumable materials used in the culturing operation into a solid waste device.

Judging a preset state of cells to be output, if adherent cells are to be output, inoculating a cell suspension in a centrifuge tube 110 into a plurality of new culture bottles 30 according to the density value, filling a culture medium into the culture bottles 30 by a liquid treatment device 160, transferring the culture bottles 30 into a culture module 200 for culture by a clamping mechanism 50 and a manipulator 230, judging whether the growth state of the cells in the culture bottles 30 meets the passage requirement by a microscope 171 in the culture operation module 100 after a preset time, and transferring the adherent cells out of the biological tissue production system by the culture operation module 100 if the cell suspension meets the passage requirement; if the preset state to be output is the cells in the frozen state, pouring the supernatant of the centrifugal cell liquid in the step, filling the frozen solution in the centrifuge tube 110 through the liquid processing device 160, forming the cell suspension in the centrifuge tube 110 through the suction and spitting action of the pipetting mechanism 70 and the pipetting gun head 111, detecting to obtain the density value of the cell suspension, inoculating the cell suspension in the centrifuge tube 110 into a plurality of new frozen storage tubes 22 according to the density value, transferring the frozen storage tubes 22 into the program cooling module 300 through the combined action of the batch grabbing mechanism 60 and the transferring mechanism 340 for cooling, transferring the frozen storage tubes 22 outside the biological tissue production system through the culture operation module 100 after the preset time, transferring the frozen storage tubes 22 outside the biological tissue production system through the batch grabbing mechanism 60, or storing the frozen storage tubes 323 in the ultralow temperature storage mechanism 320.

If the cells to be expanded are the cells in the frozen state in the frozen storage tube 22, transferring the frozen storage tube 22 to the thawing and uncapping device 120 by the batch grabbing mechanism 60, thawing and uncapping the frozen storage tube 22, taking out the centrifuge tube 110 from the consumable storage device 150 by the clamping mechanism 50, transferring the cell strain in the frozen storage tube 22 into the centrifuge tube 110 through the pipetting mechanism 70 and the pipetting gun head 111, and centrifuging by the centrifuge 172 to obtain a centrifuged cell sap; obtaining a cell suspension of the centrifuge tube 110, and detecting and recording a density value of the cell suspension, wherein the above operation process of amplifying the cell to be an adherent cell can be referred to, and the preset state of the cell to be output can be judged, and the cell in a frozen state can be output, or the adherent cell can be referred to in the above steps.

It should be noted that, if the adherent cells to be expanded do not meet the passage requirement, the culture flask 30 inoculated with the cell strain to be expanded is directly sent into the culture module 200 for continuous culture, after a preset time passes through each time of the culture flask 30 in the culture process, the microscope 171 judges whether the cells in the culture flask 30 meet the passage requirement and also displays whether the culture medium needs to be replaced, if so, the clamping mechanism 50 pours the waste culture medium in the culture flask 30 into a device of liquid waste, then transfers the culture flask 30 to the liquid treatment device 160, and pours the culture medium recovered to normal temperature by the liquid treatment device 160 in the refrigerator 170 into the culture flask 30, and the consumable used in the culture operation process pours into a device of solid waste.

The method for producing the biological tissue production system further comprises combining the functional module groups before obtaining the centrifugal cell fluid, and disposing the consumable to be used in the consumable management module 400, wherein the consumable management module 400 distributes the consumable to the consumable storage device 150, and the consumable management module 400 can continuously supply the consumable to the biological tissue production system.

The above embodiments are only preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, but any insubstantial changes and substitutions made by those skilled in the art on the basis of the present invention are intended to be within the scope of the present invention as claimed.

Claims

1. A biological tissue production system, comprising: at least one incubation operating module (100), at least one incubation module (200), at least one program cooling module (300), and at least one consumable management module (400); the modules are mechanically connected through a fixed interface (910) and electrically connected through a control interface (900); the culture operation module (100) is used for simulating manual operation and realizing automation of cell culture; the culture module (200) is used for culturing cells; the program cooling module (300) performs program cooling and low-temperature storage on a sample to be frozen; the consumable management module (400) provides various required consumables for the operation of the biological tissue production system;

The culture operation module (100) comprises a culture storage mechanism (10) for inoculating cell strains, a thawing cover opening device (120) for thawing and opening the frozen culture storage mechanism (10), and a clamping and moving device for clamping the culture storage mechanism;

The culture storage mechanism (10) comprises a freezing assembly (20), wherein the freezing assembly (20) comprises a freezing shell (21) and a freezing tube (22) arranged in the freezing shell (21);

The clamping and moving device comprises a batch grabbing mechanism (60), the batch grabbing mechanism (60) comprises a grabbing piece (62) used for clamping the freezing storage shells (21) and opening the upper covers of the freezing storage shells (21), and a grabbing pipe fitting (63) used for transferring the freezing storage pipes (22) to the defrosting and uncovering device (120), and the defrosting and uncovering device (120) is used for defrosting and uncovering the freezing storage pipes (22).

2. The biological tissue production system of claim 1, wherein the culture operation module (100) comprises: a consumable storage device (150) provided with a centrifuge tube (110), a clamping and moving device for clamping the centrifuge tube (110), and a liquid treatment device (160) for treating and moving the reagent; the clamping and moving device further comprises a clamping mechanism (50) and a liquid moving mechanism (70), wherein the clamping mechanism (50) is used for operating the culture bottle (30) and the centrifuge tube (110) of the culture storage mechanism (10).

3. The biological tissue production system of claim 1, wherein the culture module (200) comprises: a culture box body, a rotary culture tray (210) at least partially arranged in the culture box body, an automatic door (220) arranged on the culture box body and a manipulator (230) arranged outside the automatic door (220); the culture box body is provided with a culture cavity (204) and a first accommodating cavity (205) which is arranged at intervals with the culture cavity (204), the sensor assembly (201) is arranged in the first accommodating cavity (205), one end of the first air duct (202) is communicated with the culture cavity (204), the other end of the first air duct is communicated with the first accommodating cavity (205), one end of the second air duct (203) is communicated with the first accommodating cavity (205), and the other end of the second air duct is communicated with the culture cavity (204).

4. The biological tissue production system of claim 1, wherein the program cooling module (300) comprises: a program cooling mechanism (310) and an ultra-low temperature storage mechanism (320) for cooling the frozen sample; the program cooling mechanism (310) realizes ordered step cooling by transferring between any two adjacent constant-temperature freezing blocks (311) through the circulating mechanism (330) and the temperature guide blocks (312) relative to the circulating motion of each constant-temperature freezing block (311), and flexibly adds a new sample to be frozen into a freezing well of the temperature guide blocks (312) while carrying out step cooling, or places the sample to be frozen into the temperature guide blocks (312) with corresponding set temperatures according to the initial temperature of the newly added sample to be frozen; the ultralow temperature storage mechanism (320) is used for storing the frozen sample cooled by the program cooling mechanism (310).

5. The biological tissue production system of claim 1, wherein the consumable management module (400) comprises: a carrier (410) for setting up consumables, a clamping assembly (420) for transferring the consumables; the carrier frame (410) is arranged in a rotating or static way, the carrier frame (410) is driven by a motor when rotating, and the rest space on the locking support table (411) is used for installing a carrier (412) in the rotating process of the carrier frame (410); when a type of consumable is required to be prepared, the first sensor (431) identifies the in-place installation condition of the carrier (412), the identification piece on the clamping component (420) identifies the consumable, and the consumable is transferred to the target position by the clamping component (420) after confirmation.

6. The biological tissue production system according to claim 1, further comprising a housing (500) and an environmental control mechanism (600), wherein the modules are arranged in the housing (500) in a one-to-one correspondence, and the environmental control mechanism (600) is embedded in the modules; the environment control mechanism (600) comprises a regulating box (601) arranged at the top of the shell (500), a first primary filter (602) arranged in the regulating box (601) and communicated with the outside, a first centrifugal fan (603) arranged in the regulating box (601), a first efficient filter (604) communicated with the inside of the shell (500), a first temperature and humidity controller (606), a first sterilization actuator (607) and a first sensor group (605) arranged on the regulating box (601).

7. The biological tissue production system according to claim 1, further comprising a manipulation module (700), wherein the manipulation module (700) is mechanically connected to the culture operation module (100), the culture module (200), the program cooling module (300) and the consumable management module (400) through the fixed interface (910), and is electrically connected to the culture operation module (100), the culture module (200), the program cooling module (300) and the consumable management module (400) through the control interface (900); the control module (700) comprises a positioning column (701), an electric control cabinet (702) fixed on the positioning column (701), a torsion shaft (704) rotatably arranged on the positioning column (701), and a control panel (703) fixedly connected with the torsion shaft (704).

8. The biological tissue production system of claim 1, further comprising an external environmental control module (800), the external environmental control module (800) comprising a housing (801), a divider plate (802), a second primary filter (803), a second centrifugal fan (804), a second temperature and humidity controller (805), a second sterilization actuator (806), a second sensor group (807), an air supply duct (808), and a second high efficiency filter (809); the utility model provides a fan housing, including fan housing (801), air inlet bin, first high-efficient filter (803) and air supply pipeline (808), division board (802) separate into wind pressure bin and below of top with fan housing (801), second preliminary effect filter (803) install the side at fan housing (801) perpendicularly, second sensor group (807) be located the position that the pressure chamber led to air supply pipeline (808), air supply pipeline (808) connect pressure chamber and each module to through air supply pipeline (808) with the clean high-pressure air current that has handled through external environmental control module (800) carry each module, second high-efficient filter (809) be located air supply pipeline (808) and reach the end and the top of each module.

9. A method of producing a biological tissue production system according to any one of claims 1 to 8, comprising the steps of:

S1: according to the cell culture conditions in the culture storage mechanism (10), the culture operation module (100), the culture module (200), the program cooling module (300), the consumable management module (400) and the control module (700) are connected with each other through the respective fixed interface (910) and the control interface (900) to form a complete biological tissue production system;

S2: starting respective environment control mechanisms (600) of the culture operation module (100), the culture module (200), the program cooling module (300) and the consumable management module (400), sterilizing a biological tissue production system, and starting temperature and humidity control of the environment control mechanisms (600) after the sterilization is completed;

s3: spraying or pasting two-dimension codes/one-dimension codes on consumable materials to be used in a consumable material management module (400), and setting operation logic and operation programs of the biological tissue production system in a control module (700) according to the cell types required to be cultured and the expected output cell quantity;

S4: the cell strain used for culturing is cells cultured in an adherence manner in a culture flask (30), frozen cells stored in a freezing tube (22) or cells stored in equipment; cells enter the biological tissue production system from the outside to the inside through the culture operation module (100); transferring the cell fluid in the culture storage mechanism (10) into a centrifuge tube (110) for centrifugal treatment to obtain centrifugal cell fluid; pouring the supernatant of the centrifugal cell sap, and filling a reagent into the centrifuge tube (110) for treatment to obtain a cell suspension; detecting a density value of the cell suspension, inoculating the cell suspension into a new culture storage mechanism (10) according to the density value, and judging a preset state of cells to be output; if the frozen cells are output in a preset mode, transferring the new culture storage mechanism (10) to a program cooling module (300) for cooling, transferring to an ultralow temperature storage mechanism (320) for ultralow temperature preservation, and transferring the frozen cells to the outside of the biological tissue production system through a culture operation module (100) after the preset time; if the adherent cells are preset to be output, transferring the new culture storage mechanism (10) into a culture module (200) for cell culture, and judging whether the growth state of the cells meets the passage requirement after the culture is performed for a preset time; and if the cultured cells meet the passage requirement, carrying out passage operation on the adherent cells by a culture operation module (100) or transferring the adherent cells to the outside of the biological tissue production system.

10. The production method according to claim 9, wherein in S1, an external environmental control module (800) may be added as needed; s3, the consumable comprises a culture bottle (30), a centrifuge tube (110), a freezing tube (22) and a pipetting gun head box; in S4, the cell culture can be observed in real time using a microscope (171).