JP2016086751A - Reaction promoting apparatus, and nucleic acid examination apparatus - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a reaction promoting apparatus and a nucleic acid examination apparatus for amplifying nucleic acid at high speed and stably.SOLUTION: A nucleic acid examination apparatus 100 has: high-temperature heater parts 194A, 194B which are adjusted to temperature on a high-temperature side of a thermal cycle; low-temperature heater parts 195A, 195B which are adjusted to temperature on a low-temperature side; and temperature-lowering cooler parts 193A, 193B for quickly cooling a reaction vessel from a high-temperature side to a low-temperature side. In temperature control blocks, target temperatures given to reaction liquid in the thermal cycle are set. The nucleic acid examination apparatus 100, by moving the reaction vessel held by a holding part in a rotation direction around a rotation axis to allow the vessel to face the temperature control blocks in order, a thermal cycle of constant temperature is given from the temperature control blocks. Further, because the reaction vessel is quickly cooled by passing through the temperature-lowering cooler parts 193A,193B, nucleic acid amplification can be performed in shorter time.SELECTED DRAWING: Figure 6

Description

  The present disclosure relates to a reaction promoting device for amplifying a nucleic acid at high speed and a nucleic acid testing device for amplifying a nucleic acid to detect with high sensitivity, and in particular, for amplifying a nucleic acid at high speed and stably Related to technology.

  In recent years, in various fields such as genetic engineering and enzyme engineering, a technique for amplifying a small amount of nucleic acid, which is a gene, has been developed for genetic testing. For example, as a technique for selectively amplifying a specific region of a gene, a polymerase chain reaction method (PCR (polymerase chain reaction) method) or the like is known. When PCR is used, a specific DNA (deoxyribonucleic acid) region can be amplified in a short time. Millions of replicated DNA fragments can be generated from the template DNA.

  Nucleic acid amplification methods such as PCR and ligase chain reaction (LCR) amplify a known gene sequence by applying a heat cycle to the reaction solution and repeatedly heating and cooling the reaction solution. In this method, the amplified gene sequence is detected. Such nucleic acid amplification is preferably carried out in a short time in practice. For example, International Publication No. 2008/146754 (Patent Document 1 below) describes a reaction accelerator for applying a heat cycle to a reaction solution to promote a heat cycle reaction.

International Publication No. 2008/146754

  According to the technique described in Patent Document 1, in order to promote the thermal cycle reaction such as PCR, the temperature of the thermal cycle heater for applying the thermal cycle to the reaction solution is separated from the target temperature of the reaction solution. This speeds up nucleic acid amplification. However, since the temperature of the heat cycle heater is far from the target temperature of the reaction solution, nucleic acid amplification may not be stable. An object of the present disclosure is to provide another technique for amplifying nucleic acid at high speed and stably.

  According to one embodiment, a reaction promoting device is provided that amplifies nucleic acids in a reaction solution by applying a thermal cycle to the reaction solution in which a specimen and a reagent are mixed. The reaction promoting device includes a holding unit configured to hold one or more reaction vessels in which a reaction solution is accommodated, and at least first, second, and third temperature control blocks, and each of them according to a set temperature. A heater unit that regulates the temperature of the temperature control block to a constant temperature, and a control unit configured to drive the holding unit to oppose the reaction vessel and each temperature control block so as to give a thermal cycle to the reaction solution. . In the thermal cycle, the control unit adjusts the first temperature control block by the first temperature on the high temperature side given to the reaction liquid, and adjusts the second temperature control block by the second temperature on the low temperature side given to the reaction liquid. And a temperature control unit that adjusts the third temperature control block by a third temperature that is lower than the second temperature, and a holding unit that drives the reaction vessel to the first, third, and second temperature control blocks in order. And a drive control unit that applies a thermal cycle to the reaction liquid according to the first and second temperatures.

  According to an embodiment, the control unit causes the reaction container and the third temperature control block to face each other because the time when the reaction container and the third temperature control block face each other is opposed to the third temperature control block. The temperature of the reaction vessel falls within a time range in which the reaction vessel does not fall below the second temperature.

  According to one embodiment, the holding part has a disk shape, has a rotating shaft, and is configured such that the reaction vessel can move in the circumferential direction around the rotating shaft according to the drive control of the drive control unit. Each temperature control block of the heater section is arranged in order of the first, third, and second temperature control blocks on the circumference around the rotation axis so as to face the reaction vessel. The drive control unit of the control unit is configured to cause the reaction vessel to sequentially face the first, third, and second temperature control blocks by controlling the angular velocity of rotation of the rotation shaft of the holding unit.

  According to one embodiment, the drive control unit of the control unit moves the reaction container to a position facing the first temperature control block by rotation, and opposes the reaction container and the first temperature control block over a first time. And, after the first time has elapsed, the reaction container is moved by rotation to a position facing the second temperature control block, and is held so that the reaction container and the second temperature control block face each other for a second time. Drive part.

  According to one embodiment, the one or more reaction vessels are configured to be held in a region less than one half of the circumference centered on the rotation axis of the holding unit. The first and second temperature control blocks have a size corresponding to the region where the reaction vessel is held and are arranged on the circumference centering on the rotation axis so as to face the reaction vessel.

  According to one embodiment, the one or more reaction vessels are configured to be held in a region that falls within one third of the circumference around the rotation axis of the holding unit. The first and second temperature control blocks have a size corresponding to the region where the reaction vessel is held and are arranged on the circumference centering on the rotation axis so as to face the reaction vessel.

  According to one embodiment, the temperature controller of the controller adjusts the first temperature control block by a first temperature in the range of 90 degrees to 110 degrees, and adjusts the second temperature control block from room temperature to 80 degrees. The third temperature adjustment block is adjusted by a third temperature in the range of −30 degrees to room temperature.

  According to one embodiment, the heater unit includes a first temperature control block, a second temperature control block, a third temperature control block, and a fourth temperature control block, and the temperature adjustment unit of the control unit is a second temperature control block. The fourth temperature control block is adjusted by a fourth temperature that is greater than the first temperature and less than the first temperature. The drive control unit drives the holding unit to sequentially oppose the reaction vessel to the first, third, second, and fourth temperature control blocks, thereby performing the heat cycle according to the first, second, and fourth temperatures. Give to reaction.

  According to one embodiment, the temperature controller of the controller adjusts the first temperature control block by a first temperature in the range of 90 degrees to 110 degrees, and adjusts the second temperature control block from room temperature to 80 degrees. The third temperature control block is adjusted by the third temperature in the range of -30 degrees to room temperature, and the fourth temperature control block is in the range of 60 degrees to 80 degrees. The fourth temperature is adjusted.

  According to another embodiment, a nucleic acid test apparatus for performing a nucleic acid amplification test by amplifying a nucleic acid in a reaction solution by applying a thermal cycle to a reaction solution in which a sample and a reagent are mixed, and irradiating the reaction solution with excitation light Is provided. The nucleic acid test apparatus includes a holding unit configured to hold one or more reaction containers in which a reaction solution is accommodated, and at least first, second, and third temperature control blocks. A heater unit that regulates the temperature control block to a constant temperature, a control unit configured to apply a thermal cycle to the reaction liquid by driving the holding unit to oppose the reaction vessel and each temperature control block, and reaction And a detection unit configured to detect a nucleic acid amplified in the liquid. The control unit adjusts the first temperature control block by the first temperature on the high temperature side given to the reaction liquid in the thermal cycle, and controls the second temperature control block by the second temperature on the low temperature side given to the reaction liquid in the thermal cycle. Adjusting the third temperature control block by a third temperature lower than the second temperature, and driving the holding unit to drive the reaction vessel to the first, third and second temperature control blocks And a drive control unit that applies a thermal cycle to the reaction liquid at the first and second temperatures. The detection unit detects nucleic acid amplified in the reaction solution while the holding unit moves the reaction container from the second temperature control block to the first temperature control block.

  According to one embodiment, the detection unit includes an excitation light irradiator that irradiates the reaction liquid in the reaction vessel with excitation light, and a fluorescence detector that detects fluorescence generated by the excitation light irradiated by the excitation light irradiator. The detection unit detects the nucleic acid because the excitation light irradiator irradiates the reaction vessel with excitation light while the reaction unit is moved from the second temperature control block to the first temperature control block by the holding unit. The detector includes detecting fluorescence generated by excitation light irradiated to the reaction vessel.

  According to one embodiment, the heater unit includes a first temperature control block, a second temperature control block, a third temperature control block, and a fourth temperature control block. The temperature adjustment unit of the control unit adjusts the fourth temperature control block by a fourth temperature that is larger than the second temperature and smaller than the first temperature, and the drive control unit drives the holding unit to move the reaction vessel to the first temperature. By facing the first, third, second, and fourth temperature control blocks in order, a thermal cycle is given to the reaction solution by the first, second, and fourth temperatures.

  According to one embodiment, the temperature controller of the controller adjusts the first temperature control block by a first temperature in the range of 90 degrees to 110 degrees, and adjusts the second temperature control block from room temperature to 80 degrees. The third temperature control block is adjusted by the third temperature in the range of -30 degrees to room temperature, and the fourth temperature control block is in the range of 60 degrees to 80 degrees. The fourth temperature is adjusted.

  According to one embodiment, each temperature control block is temperature-controlled to a target temperature given to the reaction solution in the thermal cycle, and the reaction vessel is opposed to each temperature control block, so that the reaction solution is kept at the target temperature in the thermal cycle for a certain period of time. Thus, the nucleic acid can be amplified at high speed and stably.

  The above and other objects, features, aspects and advantages of the present invention will become apparent from the following detailed description of the present invention taken in conjunction with the accompanying drawings.

1 is an external view of a nucleic acid test apparatus 100. FIG. FIG. 3 is an exploded view of a configuration in which a reaction vessel is held by a holding plate and faces a heater unit. It is a figure which shows arrangement | positioning of the mechanism which drives the holding | maintenance part holding a reaction container, and the heater part for giving a thermal cycle to a reaction liquid. It is a figure which shows arrangement | positioning of the mechanism which drives the holding | maintenance part holding a reaction container, and the heater part for giving a thermal cycle to a reaction liquid. It is a figure which shows the cross section of a holding | maintenance part and a temperature control block. 1 is a block diagram illustrating a configuration of a nucleic acid test apparatus 100. FIG. It is a figure which shows the data structure of the heat cycle operation setting 161 memorize | stored in the memory | storage part. FIG. 4 is a diagram showing an example in which a thermal cycle is given to a reaction solution 310 by the rotational movement of a reaction vessel 300. FIG. 4 is a diagram showing an example in which a thermal cycle is given to a reaction solution 310 by the rotational movement of a reaction vessel 300. FIG. 4 is a diagram showing an example in which a thermal cycle is given to a reaction solution 310 by the rotational movement of a reaction vessel 300. FIG. 4 is a diagram showing an example in which a thermal cycle is given to a reaction solution 310 by the rotational movement of a reaction vessel 300. FIG. 4 is a diagram showing an example in which a thermal cycle is given to a reaction solution 310 by the rotational movement of a reaction vessel 300. FIG. 4 is a diagram showing an example in which a thermal cycle is given to a reaction solution 310 by the rotational movement of a reaction vessel 300. It is a figure which shows a response | compatibility with the temperature of the reaction liquid 310, and the angular velocity which rotates the holding | maintenance board 189. FIG.

  Hereinafter, embodiments of the present invention will be described with reference to the drawings. In the following description, the same parts are denoted by the same reference numerals. Their names and functions are also the same. Therefore, detailed description thereof will not be repeated.

<Embodiment 1>
FIG. 1 is a diagram showing the appearance of the nucleic acid test apparatus 100. As shown in FIG. 1, the nucleic acid test device 100 and the PC 200 are connected to each other.

  The nucleic acid test apparatus 100 has a function as a reaction promoting apparatus that amplifies the nucleic acid in the reaction liquid by applying a thermal cycle to the reaction liquid. The nucleic acid test apparatus 100 amplifies the nucleic acid by, for example, the PCR method. In the PCR method, an enzyme reaction by DNA polymerase is used to selectively amplify a part of DNA. Specifically, (1) the reaction liquid in which the specimen and the reagent are mixed is set at, for example, about 94 ° C. to 98 ° C., kept at a temperature for a predetermined time (eg, about 1 to 10 seconds), and thermally denatured. Double-stranded DNA is denatured into single-stranded DNA. (2) In order to allow DNA polymerase to act on single-stranded DNA, it is necessary to previously bind a primer to the DNA, and this annealing is performed at, for example, about 50 ° C to 70 ° C. Therefore, the nucleic acid test apparatus 100 rapidly cools the reaction solution to about 60 ° C., and anneals the single-stranded DNA and the primer. (3) Next, DNA is elongated by reacting the DNA polymerase in a temperature zone suitable for the activity of the DNA polymerase without separation of the primers. The DNA elongation may be performed at the same temperature as (2), or may be performed at a temperature higher than (2) by heating, and the required time may be, for example, about 30 seconds to 1 minute.

  Since the nucleic acid test apparatus 100 gives a thermal cycle to the reaction solution at a constant temperature for a certain period of time, the setting of the temperature given in the thermal cycle and the setting of the time for maintaining each temperature are accepted via the PC 200. In the nucleic acid test apparatus 100, the reaction containers are described as “container holding hole 187” generically referring to the container holding holes 187 A to 187 I (hereinafter referred to as the container holding holes 187 A to 187 I) provided in the holding plate 189. The holding plate 189 is rotated and stopped according to the setting, and sequentially opposed to a plurality of heaters built in the nucleic acid test apparatus 100. The temperature sensor 182 is, for example, a thermocouple, is disposed in the vicinity of the reaction vessel, and measures the temperature of the reaction vessel. The origin sensor 183 is a sensor for detecting a position serving as an origin for defining the amount of rotation of the holding plate 189 (angle rotated from the origin). For example, the origin of the holding board 189 is set by disposing a sensor for causing the origin sensor 183 to detect the origin at a position that becomes the origin. Thereby, the nucleic acid test apparatus 100 controls the rotation amount of the holding plate 189 by the signal output from the origin sensor 183 and manages the temperature of the reaction vessel by the temperature sensor 182, while maintaining the target temperature in the thermal cycle for a certain period of time. The reaction solution can be subjected to a thermal cycle, and the nucleic acid can be amplified at high speed and stably.

The nucleic acid test apparatus 100 has a mechanism for detecting the nucleic acid in the reaction container. The nucleic acid test apparatus 100 according to the first embodiment tests a nucleic acid by a fluorescence method. That is, the nucleic acid test apparatus 100 irradiates the reaction solution with excitation light from an irradiator, and detects fluorescence generated by the excitation light with a detector. Note that the method for detecting a nucleic acid is not limited to the fluorometric method.

<Structure of holding part for holding reaction vessel and heater part for giving thermal cycle to reaction liquid>
FIG. 2 is an exploded view of a configuration in which the reaction vessel is held by the holding plate and faces the heater unit.

  As shown in FIG. 2, the reaction container 300A to the reaction container 300I (hereinafter, the reaction container 300A to the reaction container 300I may be collectively referred to as “reaction container 300”) are used to collect a sample and a reagent from above. It is configured to be able to be thrown in, and the diameter becomes smaller as it goes from the upper part to the lower part. The specimen and the reagent are mixed in the reaction solution 310 at the lower part of the reaction vessel 300. The reaction vessel 300 has a tapered structure at the top, and the reaction vessel 300 is held on the holding plate 189 by the tapered structure.

  By holding the reaction vessel 300 on the holding plate 189, the reaction solution 310 and the heater unit face each other. The nucleic acid test apparatus 100 includes a plurality of temperature control blocks in the heater unit. Each temperature control block adjusts the temperature to a constant temperature according to the set temperature. Specifically, the nucleic acid test apparatus 100 includes a high temperature side temperature control block for heat-denaturing double-stranded DNA into single-stranded DNA, and a low temperature side temperature control block for annealing. And a temperature control block on the temperature lowering side for rapidly cooling the reaction solution. The nucleic acid test apparatus 100 controls the temperature of the temperature control block on the high temperature side to 90 ° C. to 110 ° C., preferably about 94 ° C. to 98 ° C. In addition, the nucleic acid test apparatus 100 adjusts the temperature control block on the low temperature side to room temperature to 80 ° C., preferably about 60 ° C., and controls the temperature control block on the temperature decrease side to −30 ° C. to about room temperature.

  In the example of FIG. 2, a low temperature heater unit 195A and a low temperature heater unit 195B are shown as temperature control blocks on the low temperature side. In a state where the reaction vessel 300 is held on the holding plate 189, the reaction liquid 310 in the reaction vessel 300 faces the low temperature heater unit 195A and the low temperature heater unit 195B. Thereby, the nucleic acid test apparatus 100 gives the reaction solution 310 a temperature of about 60 ° C. by the low temperature heater unit 195A and the low temperature heater unit 195B.

<Arrangement of mechanism and temperature control block for driving holding unit>
FIG. 3 and FIG. 4 are diagrams showing a mechanism for driving a holding unit that holds a reaction vessel, and an arrangement of a heater unit for applying a thermal cycle to the reaction solution.

  FIG. 3 is a view showing the upper surface of the holding plate 189. FIG. 4 is a diagram showing each temperature control block and detection mechanism arranged below the holding plate 189 in the nucleic acid test apparatus 100.

  In FIG. 3, the rotating disk 188 and the holding disk 189 are fixed by screwing or the like. The rotation speed control device 192 is for controlling the angular speed of rotation of the rotating disk 188 and the holding disk 189 fixed thereto. The rotating disk 188 and the rotation speed control device 192 constitute, for example, a spur gear, and the rotation disk 188 is driven in the rotation direction around the rotation axis by the rotational force of the rotation speed control device 192. As a result, the reaction vessel 300 held by the vessel holding hole 187 moves in the rotation direction about the rotation axis of the holding plate 189.

  As shown in FIG. 4, each temperature control block is arrange | positioned on the circumference. The temperature lowering cooler unit 193A and the temperature lowering cooler unit 193B are temperature control blocks on the temperature lowering side. The high temperature heater part 194A and the high temperature heater part 194B are high temperature side temperature control blocks. The low temperature heater unit 195A and the low temperature heater unit 195B are temperature control blocks on the low temperature side. By holding the reaction vessel 300 in the vessel holding hole 187, the reaction liquid 310 in the reaction vessel 300 faces these temperature control blocks. When the rotation speed control device 192 controls the angular speed of rotation, the reaction liquid 310 in the reaction vessel 300 held on the holding plate 189 moves in the rotation direction. Thereby, the nucleic acid test apparatus 100 causes the reaction solution 310 to sequentially face the high temperature heater unit 194A and the high temperature heater unit 194B, the temperature lowering cooler unit 193A and the temperature lowering cooler unit 193B, and the low temperature heater unit 195A and the low temperature heater unit 195B. Then, a heat cycle is given to the reaction liquid 310 by the high temperature heater unit 194A and the high temperature heater unit 194B, and the low temperature heater unit 195A and the low temperature heater unit 195B. The temperature-decreasing cooler unit 193A and the temperature-decreasing cooler unit 193B, which are temperature-control blocks on the temperature-decreasing side, are the reaction liquid 310 whose temperature is adjusted to about 95 ° C. by the high-temperature heater unit 194A and the high-temperature heater unit 194B, which are temperature-control blocks on the high-temperature side. This is for rapidly cooling to the temperature given to the reaction liquid 310 by the temperature control block on the low temperature side. Thereby, the time for lowering the reaction solution 310 from the high temperature side to the low temperature side can be shortened, and the time required for nucleic acid amplification can be shortened.

  The nucleic acid test apparatus 100 performs a nucleic acid amplification test using the excitation light irradiation unit 196 and the fluorescence detection unit 197. The excitation light irradiation unit 196 irradiates the reaction liquid 310 with excitation light. The fluorescence detection unit 197 detects fluorescence generated by the excitation light irradiated by the excitation light irradiation unit 196. In the nucleic acid test apparatus 100, a reflector 198 is installed between the low temperature heater unit 195A and the low temperature heater unit 195B and the high temperature heater unit 194A and the high temperature heater unit 194B. The excitation light irradiated from the excitation light irradiation unit 196 is reflected by the reflector 198. The nucleic acid test apparatus 100 moves the reaction liquid 310 from the position of the low temperature heater unit 195A and the low temperature heater unit 195B, which are low temperature side temperature control blocks, to the high temperature heater unit 194A and the high temperature heater unit 194B, which are high temperature side temperature control blocks. In the meantime, the inspection by the excitation light irradiation unit 196 and the fluorescence detection unit 197 is performed.

  The slip ring 181 is for supplying power to the rotating body and transmitting / receiving signals. For example, the slip ring 181 supplies power to the temperature sensor 182 and the origin sensor 183 of the holding plate 189, and the temperature sensor 182 and origin sensor 183 Is transmitted to the control circuit.

  FIG. 5 is a diagram illustrating a cross section of the holding unit and the temperature control block. The cross-sectional view shown in FIG. 5 shows a cross section taken along line IV-IV ′ shown in FIG. 3.

  As shown in FIG. 5, the holding plate 189 is fixed to the rotating plate 188, and the holding plate 189 is also rotated according to the rotation of the rotating plate 188. The holding plate 189 receives the insertion of the reaction vessel 300 from the upper part of the vessel holding hole 187. The reaction vessel 300 is held on the holding plate 189 by the tapered portion. The reaction liquid 310 in the reaction vessel 300 faces the low-temperature heater unit 195A and the low-temperature heater unit 195B, and is given a temperature from the low-temperature heater unit 195A and the low-temperature heater unit 195B.

  Each temperature control block of the heater unit of the nucleic acid test apparatus 100 (in FIG. 5, the high temperature heater unit 194A and the high temperature heater unit 194B, and the low temperature heater unit 195A and the low temperature heater unit 195B are shown) is screwed or the like. It is fixed to the housing.

  The nucleic acid test apparatus 100 moves the reaction container 300 from the high temperature heater unit 194A and the high temperature heater unit 194B to the positions of the low temperature heater unit 195A and the low temperature heater unit 195B by rotating the holding plate 189 in the reaction promoting unit 190.

<Configuration of Nucleic Acid Testing Device 100>
A functional configuration of the nucleic acid test apparatus 100 will be described with reference to FIG.

  FIG. 6 is a block diagram illustrating a configuration of the nucleic acid test apparatus 100. As shown in FIG. 6, the nucleic acid test apparatus 100 includes a semiconductor relay (SSR (Solid State Relay) (not shown)), a thermal fuse 104A, a thermal fuse 104B, and a thermal fuse 104C (hereinafter, thermal fuse 104A, thermal fuse 104B). And the thermal fuse 104C may be collectively referred to as the thermal fuse 104), the input / output I / F 105, the storage unit 106, the control unit 107, the first temperature controller 108A, the second temperature controller 108B, and the second 3-temperature controller 108C (hereinafter, the first temperature controller 108A, the second temperature controller 108B, and the third temperature controller 108C may be collectively referred to as the temperature controller 108), the power supply unit 109, and the reaction promotion Part 190. The reaction promoting unit 190 includes a drive unit 191, a rotation speed control device 192, temperature control blocks (a cooling cooler unit 193A and a cooling cooler unit 193B, a high temperature heater unit 194A and a high temperature heater unit 194B, a low temperature heater unit 195A, A low-temperature heater unit 195B), an excitation light irradiation unit 196, a fluorescence detection unit 197, a reflection plate 198, and a slip ring 181. In FIG. 6, a plurality of container holding holes 187 (shown as dotted circles in FIG. 6) of the holding plate 189, the origin sensor 183, and the temperature sensor 182 are indicated by dotted lines.

  The thermal fuse 104 is a heating protection member that senses the heat generated by the device due to an overcurrent caused by the occurrence of a circuit short circuit in an electrical device or a failure of a circuit component, and interrupts the circuit. The thermal fuse 104A, the thermal fuse 104B, and the thermal fuse 104C are arranged corresponding to the first temperature controller 108A, the second temperature controller 108B, and the third temperature controller 108C, respectively. The thermal fuse 104A, the thermal fuse 104B, and the thermal fuse 104C are devices generated by power supplied to each temperature control block via the first temperature controller 108A, the second temperature controller 108B, and the third temperature controller 108C. Protects various devices by detecting the heat generated and shutting off the circuit.

  The input / output I / F 105 is an input / output interface for communicating with an external device of the nucleic acid test apparatus 100, and is, for example, a general-purpose interface such as USB (Universal Serial Bus). The nucleic acid test apparatus 100 is connected to the PC 200 via the input / output I / F 105, and performs processing for nucleic acid amplification such as rotating the holding plate 189 in accordance with a program operating on the PC 200.

  The storage unit 106 is configured by a RAM (Random Access Memory) or the like, stores a program used by the nucleic acid test device 100, and accumulates various data used by the nucleic acid test device 100. In a certain aspect, the storage unit 106 stores a heat cycle operation setting 161. The heat cycle operation setting 161 is information indicating the setting of the heat cycle that the nucleic acid test apparatus 100 gives to the reaction solution 310. A plurality of temperature control blocks (in FIG. 6, the temperature on the high temperature side of the heat cycle is given to the reaction solution 310). A first temperature control block (high temperature heater unit 194A and high temperature heater unit 194B), and a second temperature control block (low temperature heater unit 195A and low temperature heater unit 195B) which gives the reaction solution 310 the temperature on the low temperature side of the thermal cycle; , Setting of the respective temperatures of the third temperature control block (showing the cooling cooler unit 193A and the cooling cooler unit 193B) for rapidly heating the reaction liquid 310 from the high temperature side temperature to the low temperature side, And setting the time for giving temperature to the reaction liquid 310 in the temperature control block.

  The control unit 107 controls the operation of the nucleic acid test apparatus 100 by reading and executing a control program stored in the storage unit 106. The control unit 107 is realized by a processor, for example. The control unit 107 operates as a drive control unit 171 and a temperature adjustment unit 172 by operating according to a program.

  The drive controller 171 controls the driving of the nucleic acid test apparatus 100 for applying a thermal cycle to the reaction solution 310. Specifically, the drive control unit 171 controls the rotation speed (angular velocity) of the rotation speed control device 192 by outputting a control signal to the drive unit 191, so that the reaction vessel 300 held by the holding plate 189 Control movement. Accordingly, the drive control unit 171 supplies the reaction liquid 310 in the reaction vessel 300 to the first temperature control block (the high temperature heater unit 194A and the high temperature heater unit 194B) and the third temperature control block (the temperature decrease cooler unit 193A and the temperature decrease cooler). Part 193B) and the second temperature control block (low-temperature heater part 195A and low-temperature heater part 195B) in order to give a thermal cycle to the reaction liquid 310.

  The temperature adjustment unit 172 adjusts the temperature of each of the plurality of temperature control blocks according to the setting of the heat cycle operation setting 161 in the heat cycle given to the reaction liquid 310. The temperature adjustment unit 172 outputs a control signal indicating the temperature of the heater unit corresponding to each temperature controller to the first temperature controller 108A, the second temperature controller 108B, and the third temperature controller 108C. For example, the temperature adjustment unit 172 controls the first temperature controller 108A corresponding to the temperature-decreasing block on the temperature-decreasing side according to the heat cycle operation setting 161 to indicate an arbitrary temperature within a range of −30 degrees to about room temperature. Output a signal. Further, the temperature adjustment unit 172 outputs a control signal indicating a temperature of about 94 ° C. to 98 ° C., for example, according to the heat cycle operation setting 161 to the second temperature controller 108B corresponding to the high temperature side temperature control block. . Further, the temperature adjustment unit 172 outputs a control signal indicating a temperature of, for example, about 60 ° C. according to the heat cycle operation setting 161 to the third temperature controller 108C corresponding to the low temperature side temperature adjustment block.

  In addition, the control unit 107 drives the excitation light irradiation unit 196 according to the position of the reaction liquid 310 to irradiate the reaction liquid 310 with the excitation light, and the fluorescence detection unit 197 detects fluorescence due to the excitation light irradiation. By accepting the detection result from the fluorescence detection unit 197, the nucleic acid amplification is inspected.

  The temperature controller 108 adjusts the temperature control block to the temperature indicated by the heat cycle operation setting 161 according to the control signal of the control unit 107. These temperature controllers 108 are operated by an AC power source.

  The first temperature controller 108A receives a signal indicating the measurement result of the temperature of the temperature-decreasing cooler unit 193B, and causes the first temperature controller 108A to adjust the temperature-decreasing cooler 193A and the temperature-decreasing cooler 193B to the temperature according to the setting. A corresponding semiconductor relay (not shown) is controlled. That is, the first temperature controller 108A controls the opening and closing of the semiconductor relay based on the measurement result of the temperature of the temperature-decreasing cooler 193B and the temperature indicated in the setting, so that the temperature-decreasing cooler unit 193A and the temperature-decreasing cooler unit 193B are connected. Control power supply.

  The second temperature controller 108B receives a signal indicating the measurement result of the temperature of the high temperature heater unit 194B, and the second temperature controller 108B so that the high temperature heater unit 194A and the high temperature heater unit 194B are adjusted to the temperature according to the setting. The semiconductor relay (not shown) corresponding to 108B is controlled. That is, the second temperature controller 108B controls the opening and closing of the semiconductor relay based on the measurement result of the temperature of the high-temperature heater unit 194B and the temperature indicated in the setting, so that the high-temperature heater unit 194A and the high-temperature heater unit 194B To control the power supply.

  The third temperature controller 108C receives a signal indicating the measurement result of the temperature of the low-temperature heater unit 195B, and the third temperature controller 195A and the low-temperature heater unit 195B are adjusted to the temperature according to the setting. A semiconductor relay (not shown) corresponding to 108C is controlled. That is, the third temperature controller 108C controls the opening and closing of the semiconductor relay based on the measurement result of the temperature of the low-temperature heater unit 195B and the temperature indicated in the setting, to the low-temperature heater unit 195A and the low-temperature heater unit 195B. To control the power supply.

  The power supply unit 109 receives AC power supplied from the outside of the nucleic acid test apparatus 100 and converts it into DC voltage.

  The drive unit 191 rotates the rotational speed control device 192 by outputting a signal indicating the angular speed of the holding plate 189 to the rotational speed control device 192 in accordance with a control signal from the control unit 107.

  The rotation speed control device 192 rotates in accordance with the drive control of the drive unit 191, and moves the reaction liquid 310 in the rotation direction by rotating the holding plate 189 by a gear mechanism.

  The temperature-decreasing cooler 193A and the temperature-decreasing cooler 193B are temperature control units that convert electrical signals into heat, and an aluminum foil or the like having a relatively high thermal conductivity is attached to the surface to give temperature to the reaction liquid 310. The temperature lowering cooler unit 193B includes a thermocouple, and outputs the temperature measured by the thermocouple to the temperature controller 108A. As a result, the first temperature controller 108A controls the supply of electric power to the cooling cooler unit 193A and the cooling cooler unit 193B, thereby adjusting the cooling cooler unit 193A and the cooling cooler unit 193B to a constant temperature.

  The high-temperature heater unit 194A and the high-temperature heater unit 194B are temperature control units that convert an electric signal into heat, and an aluminum foil or the like having a relatively high thermal conductivity is attached to the surface so that the reaction solution 310 has a high temperature side of the heat cycle. Give the temperature. The high temperature heater unit 194B includes a thermocouple, and outputs the temperature measured by the thermocouple to the temperature controller 108B. Thereby, the electric power which the 2nd temperature controller 108B supplies to the high temperature heater part 194A and the high temperature heater part 194B is controlled, and the high temperature heater part 194A and the high temperature heater part 194B are adjusted to a constant temperature.

  The low-temperature heater unit 195A and the low-temperature heater unit 195B are temperature control units that convert an electrical signal into heat, and an aluminum foil or the like having a relatively high thermal conductivity is attached to the surface so that the reaction solution 310 is heated at a high temperature side. Give the temperature. The low-temperature heater unit 195B includes a thermocouple, and outputs the temperature measured by the thermocouple to the temperature controller 108C. As a result, the power supplied from the third temperature controller 108C to the low temperature heater unit 195A and the low temperature heater unit 195B is controlled to adjust the low temperature heater unit 195A and the low temperature heater unit 195B to a constant temperature.

<Data structure>
FIG. 7 is a diagram illustrating a data structure of the heat cycle operation setting 161 stored in the storage unit 106. Referring to FIG. 7, thermal cycle operation setting 161 includes a thermal cycle setting temperature 161A and a stop time 161B.

  The heat cycle set temperature 161A indicates the setting of the temperature of each temperature control block. In the nucleic acid test apparatus 100 of the first embodiment, the temperature on the low temperature side of the heat cycle applied to the reaction solution 310 is maintained at the setting “low temperature heater temperature” of the heat cycle set temperature 161A (for example, 60 ° C.). The control unit 107 outputs a signal indicating the temperature indicated by the setting “low temperature heater temperature” to the third temperature controller 108C. The nucleic acid test apparatus 100 maintains the temperature on the high temperature side of the heat cycle at the setting “high temperature heater temperature” of the heat cycle set temperature 161A (for example, 95 ° C.). The control unit 107 outputs a signal indicating the temperature indicated by the setting “high temperature heater temperature” to the second temperature controller 108B. The nucleic acid test apparatus 100 maintains the temperature setting of the temperature lowering cooler unit 193A and the temperature lowering cooler unit 193B for rapidly cooling the reaction solution from the high temperature side temperature to the low temperature side temperature at the setting “temperature reducing cooler temperature” ( For example, 10 ° C.). The controller 107 outputs a signal indicating the temperature indicated by the setting “cooling cooler temperature” to the first temperature controller 108A.

  The stop time 161B indicates the time for stopping the reaction liquid 310 in each temperature control block. The setting “high temperature heater stop time” indicates a time during which the reaction liquid 310 is stopped while facing the high temperature heater portion 194A and the high temperature heater portion 194B. The setting “low temperature heater stop time” indicates a time for stopping the reaction liquid 310 while facing the low temperature heater portion 195A and the low temperature heater portion 195B.

  The nucleic acid test apparatus 100 accepts the settings shown in the thermal cycle operation settings 161 from the PC 200. In addition to the heat cycle operation setting 161, the nucleic acid test apparatus 100 sets the speed (angular speed) for moving the reaction liquid 310 by rotation from the PC 200 to the temperature control block on the high temperature side, and the high temperature side. And a setting of a speed (angular speed) for moving the reaction liquid 310 by rotation from the temperature control block to the temperature control block on the low temperature side.

<Operation>
The operation of the nucleic acid test apparatus 100 will be described with reference to FIGS. 8 to 13 and FIG.

  8 to 13 are diagrams showing an example in which a thermal cycle is given to the reaction liquid 310 by the rotational movement of the reaction vessel 300. FIG. 14 is a diagram illustrating the correspondence between the angular velocity for rotating the holding plate 189 and the temperature of the reaction liquid 310. In FIG. 14, the horizontal axis indicates time, and the vertical axis indicates the temperature of the reaction vessel 300 and the angular velocity at which the holding plate 189 is rotated (angular velocity at which the reaction vessel 300 rotates). In FIG. 14, the solid line indicates the measurement result of the temperature of the reaction vessel 300 (measurement result of the temperature sensor 182), and the broken line indicates the output of the angular velocity for rotating the holding plate 189 (output from the control unit 107 to the drive unit 191). Signal value of the control signal).

  FIG. 8 is a diagram showing the position of the reaction container 300 when the operation of the nucleic acid test apparatus 100 is started. As shown in FIG. 8, the nucleic acid test apparatus 100 is in a position where the reaction container 300 is heated by the low temperature heater unit 195A and the low temperature heater unit 195B at the position of the origin. The state in FIG. 8 corresponds to the time TA in FIG. In the state of FIG. 8, the temperature of the reaction vessel 300 is heated to 60 degrees. The nucleic acid test apparatus 100 operates with the angular velocity at which the reaction vessel 300 is rotated as the angular velocity “10 ° / sec”.

  FIG. 9 is a diagram illustrating the position of the reaction container 300 when the nucleic acid test apparatus 100 performs fluorescence detection. As shown in FIG. 9, the nucleic acid test apparatus 100 is configured to detect fluorescence while the reaction vessel 300 moves from the position of the low temperature heater section 195A and the low temperature heater section 195B to the position of the high temperature heater section 194A and the high temperature heater section 194B. Perform detection. The state in FIG. 9 corresponds to the time TB in FIG. As shown in FIG. 9, the reaction vessel 300 is about to move to the positions of the high-temperature heater unit 194A and the high-temperature heater unit 194B.

  FIG. 10 shows a state in which the nucleic acid test apparatus 100 has moved the reaction container 300 from the positions of the low temperature heater unit 195A and the low temperature heater unit 195B to the position of the high temperature heater unit 194A and the high temperature heater unit 194B. The control unit 107 stops the reaction vessel 300 in the state of FIG. 10 (the angular velocity for rotating the holding plate 189 is 0 ° / sec). The state of FIG. 10 corresponds to the time TC of FIG. The nucleic acid test apparatus 100 performs a hot start during the first operation of rotating the reaction vessel 300, and sets the heating time in the temperature control block (the high temperature heater unit 194A and the high temperature heater unit 194B) on the high temperature side. It is relatively long (for example, about 30 seconds).

  FIG. 11 shows a state in which the nucleic acid test apparatus 100 cools the reaction vessel 300 by the cooling cooler unit 193A and the cooling cooler unit 193B and rapidly cools from the high temperature side to the low temperature side of the thermal cycle. The nucleic acid test apparatus 100 is configured so that the temperature of the reaction vessel 300 is cooled by the cooling cooler unit 193A and the cooling cooler unit 193B when the temperature of the reaction vessel 300 is opposed to the cooling cooler unit 193A and the cooling cooler unit 193B. The time is not lower than the set temperature of the part 195B. That is, the size of the cooling cooler unit 193A and the cooling cooler unit 193B is designed so that the temperature of the reaction vessel 300 does not fall below the set temperature of the low temperature heater unit 195A and the low temperature heater unit 195B. The temperature of the cooler 193B is set. The state in FIG. 11 corresponds to the time TD in FIG.

  FIG. 12 shows a state in which the nucleic acid test apparatus 100 has moved the reaction vessel 300 from the positions of the high-temperature heater unit 194A and the high-temperature heater unit 194B to the positions of the low-temperature heater unit 195A and the low-temperature heater unit 195B. In the state of FIG. 12, the control unit 107 stops the reaction vessel 300 in accordance with the setting “low temperature heater stop time” shown in the stop time 161B. In FIG. 14, a period T2 indicates the length of time indicated by the setting “low temperature heater stop time” of the stop time 161B. The period T1 indicates the length of time indicated in the setting “high temperature heater stop time” of the stop time 161B. The state in FIG. 12 corresponds to the time TE in FIG.

  FIG. 13 is a diagram illustrating the position of the reaction vessel 300 when the nucleic acid test apparatus 100 performs fluorescence detection. The state of FIG. 13 corresponds to the time TF of FIG. Thereafter, the nucleic acid test apparatus 100 applies a thermal cycle to the reaction solution 310 by rotating the reaction vessel 300.

<Modification 1>
In the above description of the embodiment, as shown in FIG. 3, the nucleic acid test apparatus 100 is in a region that fits in a size of about one third of the circumference on the circumference around the rotation axis of the holding plate 189. The container holding holes 187A to 187I are arranged so that the reaction container 300 is held. Further, as shown in FIG. 4, the first temperature corresponding to the high temperature side of the thermal cycle is set so as to correspond to the range of the container holding hole 187A to the container holding hole 187I that is the region where the reaction vessel 300 is held. A temperature control block (high temperature heater unit 194A and high temperature heater unit 194B) and a second temperature control block (low temperature heater unit 195A and low temperature heater unit 195B) corresponding to the low temperature side of the thermal cycle are configured. The nucleic acid test apparatus 100 includes a first temperature control block, a second temperature control block, a third temperature control block (a temperature lowering cooler unit 193A and a temperature lowering cooler unit 193B), and a reflector 198 for detecting fluorescence. The area to be performed is divided into approximately three equal parts.

  In addition to this, in the nucleic acid test apparatus 100, the reaction vessel 300 is held in a region of more than one third of the circumference and less than one half on the circumference around the rotation axis of the holding plate 189. A container holding hole 187 may be arranged. In this case, the sizes of the first temperature control block and the second temperature control block are determined corresponding to the range in which the container holding hole 187 is disposed. If the region where the third temperature control block and the reflection plate 198 for detecting fluorescence can be secured, the nucleic acid test apparatus 100 can increase the number of specimens by increasing the range in which the reaction container 300 is held. Nucleic acid amplification and testing.

<Modification 2>
In the description of the above embodiment, as shown in FIG. 3 and FIG. 4, the nucleic acid test apparatus 100 includes the first temperature control block on the high temperature side, the second temperature control block on the low temperature side, and the temperature control side. Although three temperature control blocks have been described as being arranged, the number of temperature control blocks is not limited to three. For example, four temperature control blocks may be arranged, and each temperature control block may be operated at a different temperature. For example, the nucleic acid test apparatus 100 includes a first temperature control block, a second temperature control block, a third temperature control block, and a fourth temperature control block, and by rotating the holding plate 189, As the reaction vessel 300 is sequentially opposed to the first temperature control block, the third temperature control block, the second temperature control block, and the fourth temperature control block, the reaction liquid 310 may be given a heat cycle. Good. In this case, for example, the controller 107 controls the temperature of the first temperature control block in the range of 90 ° C. to 110 ° C., preferably 95 ° C., and adjusts the second temperature control block in the range of room temperature to 80 ° C., Preferably, the temperature is controlled at 60 ° C., the third temperature control block is in the range of −30 ° C. to room temperature, for example, 10 ° C., and the fourth temperature control block is in the range of 60 ° C. to 80 ° C., preferably The temperature may be controlled by 70 ° C. Thereby, the reaction liquid 310 can be heated according to the thermal cycle given to the reaction liquid 310.

<Modification 3>
In the above description of the embodiment, as shown in FIGS. 3 and 4, the nucleic acid test apparatus 100 holds the reaction vessel 300 on the circular holding plate 189 and rotates the holding plate 189, thereby 300 reaction liquids 310 have been described as facing each heater unit. However, a configuration for sequentially facing the reaction vessel 300 to each heater unit is a combination of a circular holding plate 189 and a rotation speed control device 192. Not limited to. For example, the reaction vessel 300 may move along a rectangular rail, and a heater unit may be disposed on each side of the rectangle.

  The reaction promoting device and the nucleic acid testing device according to the present embodiment are realized by a processor and a program executed thereon. The program for realizing the present embodiment may be provided by transmission / reception using a network via a communication interface.

  The embodiment disclosed this time should be considered as illustrative in all points and not restrictive. The scope of the present invention is defined by the terms of the claims, rather than the description above, and is intended to include any modifications within the scope and meaning equivalent to the terms of the claims.

  DESCRIPTION OF SYMBOLS 100 Nucleic acid test apparatus, 104 Thermal fuse, 105 Input / output I / F, 106 Memory | storage part, 107 Control part, 108 Temperature controller, 109 Power supply part, 161 Thermal cycle operation setting, 171 Drive control part, 172 Temperature control part, 181 Slip ring, 182 Temperature sensor, 183 Origin sensor, 187 Container holding hole, 188 Rotating plate, 189 Holding plate, 190 Reaction promotion unit, 191 Drive unit, 192 Rotational speed control device, 193 Temperature drop heater unit, 194 High temperature heater unit, 195 Low temperature heater section, 196 excitation light irradiation section, 197 fluorescence detection section, 198 reflector, 200 PC, 300 reaction vessel, 310 reaction liquid, 400 rotation axis.

Claims (13)

A reaction accelerator for amplifying nucleic acids in the reaction solution by applying a thermal cycle to the reaction solution in which the sample and the reagent are mixed,
A holding part configured to hold one or more reaction containers in which the reaction liquid is stored;
A heater unit that includes at least first, second, and third temperature control blocks, and adjusts each temperature control block to a constant temperature according to a set temperature;
A control unit configured to drive the holding unit to apply the thermal cycle to the reaction solution by causing the reaction vessel and each of the temperature control blocks to face each other.
The controller is
In the thermal cycle, the first temperature control block is adjusted by a first temperature on the high temperature side given to the reaction liquid, and the second temperature control block is adjusted by a second temperature on the low temperature side given to the reaction liquid. A temperature adjusting unit for adjusting and adjusting the third temperature control block by a third temperature lower than the second temperature;
The thermal cycle is given to the reaction liquid by the first and second temperatures by driving the holding unit and causing the reaction vessel to face the first, third, and second temperature control blocks in order. A reaction promoting device including a drive control unit.   The control unit makes the reaction container and the third temperature control block face each other because the time when the reaction container and the third temperature control block face each other is opposed to the third temperature control block. The reaction accelerating device according to claim 1, wherein the reaction vessel is within a time range in which the reaction vessel does not fall below the second temperature due to the temperature drop due to. The holding portion is a disc shape, has a rotation axis, and is configured such that the reaction vessel can move in the circumferential direction around the rotation axis according to the drive control of the drive control unit,
Each of the temperature control blocks of the heater unit is arranged in order of the first, third, and second temperature control blocks on the circumference around the rotation axis so as to face the reaction vessel,
The drive control unit of the control unit controls the angular velocity of rotation of the rotation shaft of the holding unit to cause the reaction vessel to sequentially face the first, third, and second temperature control blocks. The reaction promoting device according to claim 1 or 2, which is configured as described above. The drive controller of the controller is
The reaction vessel is moved by rotation to a position facing the first temperature control block so that the reaction vessel and the first temperature control block face each other for a first time,
After the first time has elapsed, the reaction vessel is moved by rotation to a position facing the second temperature control block so that the reaction vessel and the second temperature control block are opposed to each other over a second time. The reaction promoting device according to claim 3, wherein the holding unit is driven. The one or more reaction vessels are configured to be held in a region less than a half of a circumference around the rotation axis of the holding unit,
The first and second temperature control blocks have a size corresponding to the region in which the reaction vessel is held and are opposed to the reaction vessel on a circumference around the rotation axis. The reaction promoting device according to claim 3 or 4, which is arranged. The one or more reaction vessels are configured to be held in a region that falls within one third of the circumference around the rotation axis of the holding unit,
The first and second temperature control blocks have a size corresponding to the region in which the reaction vessel is held and are opposed to the reaction vessel on a circumference around the rotation axis. 6. The reaction promoting device according to claim 5, which is arranged.   The temperature adjustment unit of the control unit adjusts the first temperature control block by the first temperature in a range of 90 to 110 degrees, and adjusts the second temperature control block to a room temperature to 80 degrees. The temperature is adjusted by the second temperature in the range, and the third temperature control block is adjusted by the third temperature in the range of -30 degrees to room temperature. Reaction accelerator. The heater unit includes the first temperature control block, the second temperature control block, the third temperature control block, and a fourth temperature control block,
The temperature adjusting unit of the control unit adjusts the fourth temperature control block by a fourth temperature that is larger than the second temperature and smaller than the first temperature,
The drive control unit drives the holding unit to cause the reaction vessel to sequentially face the first, third, second, and fourth temperature control blocks, whereby the first, second, and fourth The reaction accelerator according to any one of claims 1 to 4, wherein the thermal cycle is given to the reaction solution according to temperature.   The temperature adjustment unit of the control unit adjusts the first temperature control block by the first temperature in a range of 90 to 110 degrees, and adjusts the second temperature control block to a room temperature to 80 degrees. The third temperature control block is adjusted by the third temperature in the range of −30 degrees to room temperature, and the fourth temperature control block is adjusted by 60 degrees to 80 degrees. The reaction accelerator according to claim 8, wherein the reaction accelerator is adjusted by the fourth temperature in a range of degrees. A nucleic acid test apparatus for performing a nucleic acid amplification test by amplifying a nucleic acid in the reaction solution by applying a thermal cycle to a reaction solution in which a sample and a reagent are mixed, and irradiating the reaction solution with excitation light,
A holding part configured to hold one or more reaction containers in which the reaction liquid is stored;
A heater unit that includes at least first, second, and third temperature control blocks, and adjusts each temperature control block to a constant temperature according to a set temperature;
A control unit configured to drive the holding unit to cause the reaction vessel and each of the temperature control blocks to face each other so as to give the thermal cycle to the reaction solution;
A detection unit configured to detect the nucleic acid amplified in the reaction solution,
The controller is
The first temperature control block is adjusted by a first temperature on the high temperature side given to the reaction liquid in the thermal cycle, and a second temperature control is made by a second temperature on the low temperature side given to the reaction liquid in the thermal cycle. A temperature adjusting unit for adjusting the block and adjusting the third temperature control block by a third temperature lower than the second temperature;
The thermal cycle is given to the reaction liquid by the first and second temperatures by driving the holding unit and causing the reaction vessel to face the first, third, and second temperature control blocks in order. Including a drive control unit,
The detection unit detects the nucleic acid amplified in the reaction solution while moving the reaction container from the second temperature control block to the first temperature control block by the holding unit. apparatus. The detector is
An excitation light irradiator that irradiates the reaction solution in the reaction vessel with excitation light;
A fluorescence detector for detecting fluorescence generated by the excitation light irradiated by the excitation light irradiator,
The detection unit detects the nucleic acid when the excitation light irradiator moves the reaction container from the second temperature control block to the first temperature control block by the holding unit. The nucleic acid test apparatus according to claim 10, comprising: irradiating a container with the excitation light, and the fluorescence detector detecting fluorescence generated by the excitation light irradiated on the reaction container. The heater unit includes the first temperature control block, the second temperature control block, the third temperature control block, and a fourth temperature control block,
The temperature adjusting unit of the control unit adjusts the fourth temperature control block by a fourth temperature that is larger than the second temperature and smaller than the first temperature,
The drive control unit drives the holding unit to cause the reaction vessel to sequentially face the first, third, second, and fourth temperature control blocks, whereby the first, second, and fourth The nucleic acid test apparatus according to claim 10, wherein the thermal cycle is given to the reaction solution according to temperature.   The temperature adjustment unit of the control unit adjusts the first temperature control block by the first temperature in a range of 90 to 110 degrees, and adjusts the second temperature control block to a room temperature to 80 degrees. The third temperature control block is adjusted by the third temperature in the range of −30 degrees to room temperature, and the fourth temperature control block is adjusted by 60 degrees to 80 degrees. The nucleic acid test device according to claim 12, wherein the nucleic acid test device is adjusted by the fourth temperature in a range of degrees.