CN113528341A

CN113528341A - Biological tissue production system and production method

Info

Publication number: CN113528341A
Application number: CN202110621002.2A
Authority: CN
Inventors: 赖雪聪; 倪孝杰; 徐铭恩
Original assignee: Regenovo Biotechnology Co ltd
Current assignee: Regenovo Biotechnology Co ltd
Priority date: 2021-06-03
Filing date: 2021-06-03
Publication date: 2021-10-22

Abstract

The invention relates to a biological tissue production system and a production method, wherein the system comprises at least one culture operation module, at least one culture module, at least one program cooling module and at least one consumable management module; the modules can be mechanically connected through a fixed interface and electrically connected through a control interface; the culture operation module is used for simulating manual operation, and can realize automation of cell culture; the culture module is used for culturing cells and has the functions of high-temperature sterilization and automatic transportation; the program cooling module performs program cooling and low-temperature storage on a sample needing to be frozen; the consumable management module provides various required consumables for the operation of the biological tissue production system. The technical scheme of the invention can carry out freezing or culture according to the output state of the cells, reduces the dependence on the operation level and experience of operators in production, can freely combine the number of modules according to the requirement, has extremely strong flexibility and expansibility, and meets the requirement of complex cell production.

Description

Biological tissue production system and production method

Technical Field

The invention relates to the field of biological tissue production, in particular to a biological tissue production system and a production method.

Background

Cell expansion is completed by pure manual operation or semi-automatically by means of equipment, the operation level and operation experience of operators are required to be relied on, manual production is carried out, the efficiency is low, and meanwhile, the cell expansion mode is limited due to the fact that the production architecture mode is single, and the increasingly complex cell production requirements are difficult to meet.

Disclosure of Invention

The invention aims to overcome the defects of low efficiency, dependence on artificial production and difficulty in meeting the production requirement of complex cells in the prior art, and provides a biological tissue production system.

In a first aspect, the present invention provides a biological tissue production system, comprising at least one culture operation module, at least one culture module, at least one program cooling module, and at least one consumable management module; the modules can be mechanically connected through a fixed interface and electrically connected through a control interface; the culture operation module is used for simulating manual operation, and can realize automation of cell culture; the culture module is used for culturing cells and has the functions of high-temperature sterilization and automatic transportation; the program cooling module performs program cooling and low-temperature storage on a sample needing to be frozen; the consumable management module provides various required consumables for the operation of the biological tissue production system.

Further, the culture operation module comprises a culture storage mechanism for inoculating cell strains, a thawing and cover opening device for thawing and opening the frozen culture storage mechanism, a consumable storage device provided with a centrifuge tube, a clamping and moving device for clamping the centrifuge tube and/or the culture storage mechanism, and a liquid processing device for processing and transferring reagents; the clamping and moving device comprises a batch grabbing mechanism, a clamping mechanism and a liquid moving mechanism, wherein the batch grabbing mechanism can grab culture and storage mechanisms such as a cryopreservation assembly, the clamping mechanism can operate the culture and storage mechanisms such as a culture bottle and a centrifuge tube, and the liquid moving mechanism can be used for liquid suction or liquid discharge; the unfreezing and cover opening device is matched with the batch grabbing mechanism to finish the unfreezing operation of the cryopreservation assembly.

Further, the culture module comprises a culture box body, a rotary culture disc at least partially arranged in the culture box body, an automatic door arranged on the culture box body and a manipulator outside the automatic door; the culture box body is provided with a culture cavity and a first accommodating cavity which is arranged at an interval with the culture cavity, the sensor assembly is placed in the first accommodating cavity, the culture cavity is communicated with the accommodating cavity through a first air duct and a second air duct, and the sensor assembly can be protected and the environmental state in the culture cavity can be monitored in real time during high-temperature sterilization of the culture cavity; the manipulator with rotatory cultivation dish cooperatees, realizes cultivateing the automatic of storage mechanism and transports to through the isolation effect of automatically-controlled door, the protection the manipulator avoids cultivateing the injury that cavity high temperature sterilization brought.

Furthermore, the program cooling module comprises a program cooling mechanism and an ultralow temperature storage mechanism, wherein the program cooling mechanism is used for cooling the cryopreserved sample; the program cooling mechanism realizes sequential step cooling by transferring the temperature guide blocks between any two adjacent constant-temperature freezing blocks through the circulating mechanism and the temperature guide blocks relative to the circulating motion of each constant-temperature freezing block, and can flexibly add a new sample to be frozen into the freezing well of the temperature guide block while performing step cooling or place the sample in the temperature guide block with a corresponding set temperature according to the initial temperature of the newly added sample to be frozen; the ultra-low temperature storage mechanism is used for preserving the cryopreserved samples cooled by the program cooling mechanism at low temperature for a long time.

Further, the consumable management module comprises a carrier for arranging the consumable, and a clamping assembly for transferring the consumable; the object carrier can be designed to rotate or be static, the object carrier can be driven by a motor during rotation, and the rest space on the supporting table is locked for installing objects during rotation, so that the space utilization rate is improved; when a type of consumable is required to be allocated, the first sensor identifies the in-place installation condition of the carrier, the identification piece on the clamping assembly identifies the consumable, and the consumable is transferred to the target position by the clamping piece after confirmation.

Further, the biological tissue production system further comprises a shell and an environment control mechanism, wherein the modules are correspondingly arranged in the shell one by one, and the environment control mechanism is embedded in the modules; environmental control mechanism including install in the regulation and control box at casing top, install in the regulation and control box and with the first primary filter that outside intercommunication set up, install in first centrifugal fan in the regulation and control box, with the first high efficiency filter that the inside intercommunication of casing set up, and install in first sensor group on the regulation and control box carries out the first atmospheric control ware that constant temperature and humidity handled, the first sterilization executor that can produce ozone gas or hydrogen oxide steam fast to required clean, aseptic environmental condition in guaranteeing each module.

Furthermore, the biological tissue production system also comprises a control module, wherein the control module is mechanically connected with each module through a fixed interface and is electrically connected with each module through the control interface; the control module comprises a positioning column, an electric control cabinet fixed on the positioning column, a torsion shaft rotationally arranged on the positioning column, and a control panel fixedly connected with the torsion shaft; the electric control cabinet is used for controlling the operation of the biological tissue production system, and the control panel is an interface for user interaction.

Furthermore, the biological tissue production system also comprises an external environment control module, wherein the external environment control module comprises a wind shell, a partition plate, a second primary filter, a second centrifugal fan, a second temperature and humidity controller, a second sterilization actuator, a second sensor group, an air supply pipeline and a second high-efficiency filter; the division board separates the wind shell for the wind pressure storehouse of top and the inlet air storehouse of below, the side at the wind shell is installed perpendicularly to the first filter of second, second sensor group be located the position that the pressure chamber accesss to the supply air duct for the inside environment of monitoring biological tissue production system, and give back in real time to external environmental control module guarantees biological tissue production system internal environment control's accuracy, supply air duct connect pressure chamber and above-mentioned each module to will carry each module through the clean high pressure draught that external environmental control module handled through supply air duct, second high efficiency filter be located supply air duct and reach the end and the top of each module, filterable formation accords with the perpendicular decurrent parallel air current that the cleanliness factor required.

In a second aspect, the present invention also provides a method for producing a biological tissue production system, comprising the steps of:

according to the cell culture condition in cultivateing the storage mechanism, will be no less than one cultivate operation module, be no less than one cultivate the module, be no less than one procedure cooling module, be no less than one consumptive material management module and one control module through respective fixed interface and control interface interconnect, make up into one set of complete biological tissue production system, can install external environmental control module additional as required in addition.

The respective environmental control mechanism of operation module, cultivation module, procedure cooling module and consumptive material management module is cultivateed in the start, carries out disinfection to biological tissue production system, treats the disinfection and accomplishes the back, starts environmental control mechanism's atmospheric control, guarantees that the cleanliness factor in the equipment is stable, and the environmental control function also can be carried out by external environmental control module.

To the consumptive material for use among the consumptive material management module, if spraying or pasting two-dimensional code/one-dimensional code on consumptive materials such as blake bottle, centrifuging tube, cryopreserving pipe, pipetting gun head box, the identification of the consumptive material of being convenient for, set for biological tissue production system's operation logic and operation procedure in controlling the module according to the cell type of required cultivation and the output cell volume of prediction.

The cell strain used for culture can be cells cultured in culture bottles in an adherent way, frozen cells stored in a freezing tube or cells stored in equipment; the cells enter the biological tissue production system from the outside through the culture operation module; transferring the cell sap in the culture storage mechanism into a centrifugal tube for centrifugal treatment to obtain centrifugal cell sap; pouring the supernatant of the centrifuged cell sap, and filling a reagent into the centrifugal tube for processing to obtain a cell suspension; detecting to obtain a density value of the cell suspension, inoculating the cell suspension into a new culture storage mechanism according to the density value, and judging a preset state of cells to be output; if output cryopreserved cells are preset, transferring the new culture storage mechanism to a program cooling module for cooling, transferring the new culture storage mechanism to an ultralow temperature storage mechanism for ultralow temperature preservation treatment, and transferring the cryopreserved cells to the outside of the biological tissue production system through a culture operation module after preset time; if the adherent cells are preset to be output, transferring the new culture storage mechanism to a culture module for cell culture, and judging whether the growth state of the cells meets the passage requirement after the cells are cultured for a preset time; and if the cultured cells meet the passage requirement, passage operation is carried out on the adherent cells through a culture operation module or the adherent cells are transferred out of the biological tissue production system.

Compared with the prior art, the invention has the beneficial effects that:

according to the biological tissue production system provided by the technical scheme, the at least one culture operation module, the at least one culture module, the at least one program cooling module and the at least one consumable management module are connected and communicated with one another, and based on the clamping and moving device, the unfreezing and cover opening device and the liquid processing device in the culture operation module, multiple operations can be better replaced by manual work, so that the operation efficiency is improved; the culture module has a high-temperature sterilization function and can protect the sensor assembly from being damaged by high-temperature sterilization, the manipulator and the rotary culture disc are cooperatively matched to realize automatic transfer of the culture storage mechanism, and the manipulator is protected from being damaged by high-temperature sterilization of the culture cavity through the isolation effect of the automatic door; the program cooling mechanism of the program cooling module and the ultralow temperature storage mechanism act together and are used for outputting the frozen samples; the consumable material management module is characterized in that the consumable material management module is provided with a carrier, temporary preparation is not needed, when a type of consumable material needs to be prepared, the first sensor identifies the installation in-place condition of the carrier, the identification piece on the clamping assembly identifies the consumable material, and the consumable material is transferred to a target position by the clamping piece after confirmation, so that the accuracy of consumable material preparation is improved, and the operation time for increasing errors in preparation of personnel is reduced. The biological tissue production system has complete automatic operation conditions and capacity, can be used for freezing or culturing according to the output state of cells, can continuously supply consumables, enables each module to perform closed independent operation well, is high in production efficiency, reduces the participation of personnel in the cell culture process, reduces the dependence on the operation level and experience of the personnel in production, can be set according to the number of the modules, can be freely combined according to application requirements, has extremely strong flexibility and expansibility, and meets the complex cell production requirements.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a front view of a biological tissue production system according to one embodiment of the present invention;

FIG. 2 is a front view of a biological tissue production system according to another embodiment of the present invention;

FIG. 3 is a top view of a biological tissue production system of the present invention;

FIG. 4 is a schematic view showing the internal structure of the culture operation module shown in FIG. 1;

FIG. 5 is a schematic view of the clamping mechanism and the culture bottle according to the embodiment of the present invention;

FIG. 6 is an exploded view of the clamping mechanism, the vial and the centrifuge tube of the present invention;

FIG. 7 is a schematic structural diagram of a batch grabbing mechanism and a freezing shell in an embodiment of the present invention;

FIG. 8 is a schematic structural view of the batch grabbing mechanism of FIG. 7;

FIG. 9 is a schematic structural view of the consumable storage device shown in FIG. 4;

FIG. 10 is a schematic structural view of the pipetting mechanism shown in FIG. 4;

FIG. 11 is a schematic view of a liquid treatment apparatus according to an embodiment of the present invention;

FIG. 12 is a schematic view showing the internal structure of the culture module shown in FIG. 1;

FIG. 13 is a schematic structural view of a portion of the assembly shown in FIG. 12;

FIG. 14 is a schematic diagram of an internal structure of the program cooling module shown in FIG. 1;

FIG. 15 is a schematic view of a portion of the temperature reduction mechanism of FIG. 14;

FIG. 16 is a schematic structural view of the temperature reduction mechanism of FIG. 15 from another perspective;

FIG. 17 is a schematic structural view of the ultra-low temperature storage mechanism shown in FIG. 14;

FIG. 18 is a schematic diagram of the internal structure of the consumable management module shown in FIG. 1;

FIG. 19 is a schematic view of a portion of the subassembly shown in FIG. 18;

FIG. 20 is a schematic view of an environmental control mechanism in an embodiment of the present invention;

FIG. 21 is a schematic structural diagram of the control module shown in FIG. 2;

description of reference numerals:

100. a culture operation module; 10. a culture storage mechanism; 20. freezing and storing the components; 21. freezing and storing the shells; 22. freezing and storing the tube; 30. a culture bottle; 41. a mechanical arm; 50. a clamping mechanism; 51. a first driving member; 52. a clamping group; 53. a clamping portion; 60. a batch grabbing mechanism; 61. a base shell; 62. a grasping member; 63. grabbing the pipe fitting; 64. a cylinder; 70. a pipetting mechanism; 111. a pipette tip; 120. a cover opening device is unfrozen; 150. a consumable storage device; 151. a carrier; 160. a liquid treatment device; 161. a liquid transfer pipeline; 162. a heating assembly; 163. a pump body; 164. adding a sample piece; 170. a refrigerator; 171. a microscope; 172. a centrifuge;

200. a culture module; 201. a sensor assembly; 202. a first gas guide pipe; 203. a second gas guide pipe; 204. culturing the cavity; 205. a first accommodating cavity; 210. rotating the culture tray; 220. an automatic door; 230. a manipulator;

300. a program cooling module; 310. a programmed cooling mechanism; 311. freezing the block at constant temperature; 312. a temperature conducting block; 313. a cooling well; 320. an ultra-low temperature storage mechanism; 321. a storage rack; 322. a lifting mechanism; 323. a freezing bin; 324. a refrigeration member; 330. a circulating mechanism; 340. a transfer mechanism;

400. a consumable management module; 410. a carrier; 411. a saddle; 412. carrying an object; 420. a clamping assembly; 431. a first sensor;

500. a housing;

600. an environmental control mechanism; 601. a regulatory cassette; 602. a first primary filter; 603. a first centrifugal fan; 604. a first high efficiency filter; 605. a first sensor group; 606. a first temperature and humidity controller; 607. a first sterilization actuator;

700. a control module; 701. a positioning column; 702. an electric control cabinet; 703. a control panel; 704. a torsion shaft; 705. a touch screen; 706. an alarm; 707. a signal lamp; 708. a scram switch;

800. an external environment control module; 801. a wind shell; 802. a partition plate; 803. a second primary filter; 804. a second centrifugal fan; 805. a second temperature and humidity controller; 806. a second sterilization actuator; 807. a second sensor group; 808. an air supply duct; 809. a second high efficiency filter;

900. a control interface; 910. and fixing the interface.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-21, an embodiment of the present invention provides a biological tissue production system, including: the functional module group comprises at least one culture operation module 100, at least one culture module 200, at least one program cooling module 300 and at least one consumable management module 400 which are mutually connected and communicated.

The culture operation module 100 is a main action part of the functional module group, simulates manual operation to perform various operations, the culture module 200 is a place for cell growth and differentiation, the program cooling module 300 reduces the temperature to a preset temperature at a preset rate and maintains the temperature for a period of time, the consumable management module 400 is the only window for solid consumables to enter the functional module group, and is a warehouse for the functional module group solid consumables, the functional module group has complete automatic operation conditions and capability, can be frozen or cultured according to the state of cell output, and can continuously supply consumables, so that the functional module group can perform better closed independent operation, has high production efficiency, reduces the participation of personnel in the cell culture process, reduces the dependence on the operation level and experience of the personnel in production, can be set into a plurality of functional module groups, and the number of each module in each functional module group can be set according to needs, and each module is freely combined according to application requirements, so that the method has extremely strong flexibility and expansibility, and meets the complex cell production requirements.

Each module is provided with a shell 500, the side surface of each shell 500 is provided with a fixed interface 910 and a control interface 900, the modules are mechanically connected with each other through the fixed interface 910, the control interface 900 is combined with or separated from the fixed interface 910, the control interface 900 is used for electrically connecting control lines of each module, and the fixed interface 910 can adopt the modes of screw locking, pin and pin hole locking, electromagnetic lock locking, air lock locking and the like.

The culture operation module 100 comprises a culture storage mechanism for inoculating cell strains, a thawing and cover-opening device 120 for thawing and opening a cover of the culture storage mechanism for freezing, a consumable storage device 150 provided with a centrifuge tube 110, a clamping and moving device for clamping the centrifuge tube 110 and/or the culture storage mechanism, and a liquid processing device 160 for processing and transferring reagents. The culture storage mechanism can be a cryopreservation assembly, a culture bottle, a centrifuge tube or a cryopreservation sample and the like.

It should be noted that, it can be used to centre gripping centrifuging tube 110, cultivation storage mechanism etc. to press from both sides the device of moving, cultivation storage mechanism can be conveyed inside casing 500 by the outside in process of production, also can set up inside casing 500 before producing, the cell strain that cultivation storage mechanism waited to expand can be for inoculating to the adherent cell of blake bottle 30, also can be for freezing the cell that is in frozen state in the tube 22 that deposits, and new cultivation storage mechanism is empty blake bottle 30, and obtains from consumptive material storage device 150 by pressing from both sides the device of moving, cultivates storage mechanism business turn over biological tissue production system can be accomplished by pressing from both sides the device of moving, of course also can set up other transport mechanism.

It can be understood that, as shown in fig. 4-11, the housing 500 of the cultivation operation module 100 is provided with a mechanical arm 41, the mechanical arm 41 and the clamping mechanism 50 work together to better replace manual work to perform various operations, thereby improving the work efficiency, and the carrier 410 is used to store consumables such as centrifuge tubes 110, thereby avoiding the problem of terminating operation due to shortage of consumables, reducing the participation of personnel in the cell cultivation process, and improving the production automation.

Wherein, the clamping device includes a clamping mechanism 50 for clamping the centrifuge tube 110 and/or the culture storage mechanism, the clamping mechanism 50 includes a first driving member 51, and a plurality of clamping groups 52 fixed on the first driving member 51, each clamping group 52 is clamped on the outer surface of the centrifuge tube 110 or the culture storage mechanism, each clamping group 52 includes a plurality of clamping portions 53 disposed around the side of the first driving member 51, it can be understood that the clamping mechanism 50 can be used for transferring articles between the culture operation module 100 and other modules, as shown in fig. 5-6, the first driving member 51 drives the clamping groups 52, so that the clamping portions 53 in each clamping group 52 move towards or away from each other, thereby opening or closing the clamping groups 52 is realized, the number, size, shape and the like of the clamping groups 52 can be adjusted as required, each of the clamping groups 52 may clamp a centrifuge tube 110 or a culture flask 30 or a vial 22, etc.

In one embodiment, when the cell strains to be expanded are cells in a frozen state in the freezing tube, in order to clamp and transfer the freezing tube 22 entering the functional module group, the culture storage mechanism comprises a freezing assembly 20, the freezing assembly 20 comprises a freezing shell 21 and the freezing tube 22 installed in the freezing shell 21, the clamping and transferring device further comprises a batch grabbing mechanism 60, and the batch grabbing mechanism 60 comprises a base shell 61, a grabbing piece 62 hinged to the base shell 61, and a grabbing tube piece 63 installed on the bottom surface of the base shell 61. It can be understood that one or more freezing tubes 22 can be stored in the freezing shell 21, as shown in fig. 7-8, since the gripping member 62 is hinged to the base shell 61, the gripping member 62 can rotate relative to the base shell 61, not only clamping or releasing the freezing shell 21 can be realized, but also the freezing shells 21 with different sizes can be clamped and matched, the gripping member 62 can clamp and rotate the upper cover in the freezing shell 21 to open the freezing shell 21, when the freezing tubes 22 in the freezing shell 21 need to be transferred, under the action of the mechanical arm 41, the gripping member 63 is fixed in an interference fit manner with the freezing tubes 22, so that the freezing tubes 22 fixed on the gripping member 63 are transferred to target positions, and the number and positions of the gripping members 63 correspond to the number and positions of the freezing tubes 22.

In order to take down the frozen pipe 22 fixed on the grabbing pipe 63, the batch grabbing mechanism 60 further comprises a cylinder 64 arranged on one side of the base shell 61 far away from the grabbing pipe 63, a moving member fixedly connected with the output end of the cylinder 64 and installed in the base shell 61, and a pushing member fixed on one side of the moving member far away from the cylinder 64 and arranged in the grabbing pipe 63 in a penetrating manner, wherein the cylinder 64 is used for driving the moving member to move up and down relative to the base shell 61.

In order to thaw the frozen tube in the cryopreservation assembly 20, the culture operation module 100 includes a thawing and uncovering device 120 for installing consumables such as a centrifuge tube 110, as shown in fig. 9-10, the consumable storage device 150 further includes a carrier 151, a pipette tip 111 on the carrier 151 and for transferring liquid, the gripping and transferring device further includes a pipetting mechanism 70, the pipetting mechanism 70 is matched with the pipette tip 111 and can be used for transferring liquid in cell culture processes such as cell culture in a culture bottle 30, the cryopreservation tube 22 and the centrifuge tube 110. Under the combined action of the pipetting mechanism 70 and the pipetting tip 111, the centrifuged cell fluid can become a cell suspension by repeatedly blowing the centrifuge tube 110, or the digested adherent cells in the culture flask 30 can become a cell suspension by repeatedly blowing the digested adherent cells; of course, the cells in the centrifuge tube 110 and the culture flask 30 may be shaken into a cell suspension by the cooperation of the holding mechanism 50 and the robot arm 41.

In one embodiment, the culture storage mechanism further includes a culture bottle 30 inoculated with cells, the culture operation module 100 further includes a liquid processing device 160 for transferring reagents into the culture bottle 30 or the centrifuge tube 110, as shown in fig. 11, the liquid processing device 160 includes a pipetting line 161, a heating assembly 162 and a pump body 163 both mounted on the pipetting line 161, and a sample adding member 164 communicated with one end of the pipetting line 161, and the other end of the pipetting line 161 is communicated with the culture bottle 30 or the centrifuge tube 110. The sample adding member 164 is a storage carrier of a liquid to be transferred, and may be a bottle with any volume and shape, the liquid transfer line 161 is used for transferring the liquid from the sample adding member 164 to the culture bottle 30 or a channel in the centrifuge tube 110, and under the action of the pump body 163, the liquid in the sample adding member 164 is transferred to the heating assembly 162 through the liquid transfer line 161, and is transferred to the culture bottle 30 or the centrifuge tube 110 after being heated by the heating assembly 162.

The liquid processing apparatus 160 is used to heat the biological reagent stored in the refrigerator 170 or other biological reagents at other positions before entering the centrifuge tube 110 or the culture storage mechanism, the heating unit 162 is used to return the liquid to normal temperature, and the heating unit 162 heats the liquid by means including, but not limited to, water bath heating, oil bath heating, metal bath heating, hot air heating, and the like.

In other embodiments, the culture operation module 100 may further include a microscope 171 for observing the cell culture conditions in real time, a centrifuge 172 for separating particles from the cell suspension, a refrigerator 170 for cryogenically storing the reagents, a clamping and moving installation for storing the clamping and moving device, and a device for processing liquid and solid wastes.

Cultivate module 200, including cultivateing box body, sensor module 201, air duct 202 and air duct two 203, cultivate the box body be formed with cultivate cavity 204 and with cultivate the cavity 204 interval set up hold the chamber 205, sensor module 201 set up in hold in the chamber 205, air duct 202 is worn to locate in the lateral wall of cultivateing the box body, the one end of air duct 202 communicate in cultivate cavity 204, the other end communicate in hold chamber 205, the one end of air duct two 203 communicate in hold chamber 205, the other end communicate in cultivate cavity 204.

It can be understood that, as shown in fig. 12-13, when the cell sap that is packed separately needs to be cultured, the culture storage mechanism is transferred into the culture module 200, the gas in the culture cavity 204 can flow into the first accommodating cavity 205 through the first air duct 202, the sensor module 201 detects the gas in the first accommodating cavity 205 to obtain information such as corresponding temperature, humidity, carbon dioxide concentration and the like in real time, and then the gas flowing into the first accommodating cavity 205 flows back into the culture cavity 204 provided with the culture storage mechanism through the second air duct 203. Preferably, the sensor assembly 201 may be: a temperature sensor, a humidity sensor and a carbon dioxide sensor for detecting the temperature, humidity and carbon dioxide concentration of the gas in the culture cavity 204, respectively.

In other embodiments, in order to realize the operation of moving in and taking out the culture bottle 30 inside the housing 500 provided with the culture module 200, the culture box body is provided with an automatic door 220 for opening and closing and a manipulator 230 arranged in the housing 500, the manipulator 230 and the culture box body are arranged at an interval, the manipulator 230 moves the culture storage mechanism into the culture cavity 204 or takes out the culture storage mechanism from the culture cavity 204, the manipulator 230 can also be used for transferring the culture bottle 30 into or out of the culture module 200, which is beneficial for transferring articles among modules, and the automatic door 220 component plays a role in isolating the culture cavity 204 from the manipulator 230, so that the manipulator 230 can be effectively protected in the process of carrying out high-temperature sterilization inside the culture cavity 204.

In one embodiment, the program cooling module 300 includes a program cooling mechanism 310, where the program cooling mechanism 310 includes at least two constant-temperature freezing blocks 311 with different temperatures, at least two temperature-guiding blocks 312, and a circulation mechanism 330 for fixing the temperature-guiding blocks 312, a cooling surface is formed on the same side of each constant-temperature freezing block 311, a cooling well 313 for accommodating the culture storage mechanism is formed on each temperature-guiding block 312, and any temperature-guiding block 312 is attached to any cooling surface and can move relatively.

It will be appreciated that, as shown in FIGS. 14-16, when it is desired to lower the temperature of the dispensed cellular fluids, the culture storage mechanism is transferred into the program cooling module 300, any temperature conduction block 312 can move from the refrigerating surface of one constant-temperature freezing block 311 to the refrigerating surface of the other constant-temperature freezing block 311, so that the temperature guide blocks 312 can obtain energy from the refrigerating surface to achieve the cooling effect, each temperature guide block 312 is fixedly connected to the circulating mechanism 330, the circulating mechanism 330 can rotate relative to the constant-temperature refrigerating block 311, when the circulating mechanism 330 is driven, the temperature guide blocks 312 and the circulating mechanism 330 synchronously perform circulating motion, and one temperature guide block 312 can move from the refrigerating surface of one constant-temperature refrigerating block 311 to the refrigerating surface of the adjacent constant-temperature refrigerating block 311, so that the temperature guide blocks 312, the temperature can be reduced to-80 ℃ in a step manner, and a culture storage mechanism with cell suspension is convenient to add.

In one embodiment, the above-mentioned program cooling module 300 can implement a step-type cooling by the program cooling mechanism 310, and can also implement an ultra-low temperature storage mechanism 320 to perform an ultra-low temperature storage at-196 ℃ on the cell suspension in the cryopreservation tube 22, the program cooling module 300 further includes an ultra-low temperature storage mechanism 320 for performing a long-term storage on the cell sap in the program cooling mechanism 310, and the ultra-low temperature storage mechanism 320 includes: the refrigerator comprises a storage rack 321, a lifting mechanism 322 connected to the storage rack 321, a freezing chamber 323 connected to the lifting mechanism 322, and a refrigerating element 324 fixedly arranged on the outer surface of the freezing chamber 323.

It can be understood that, as shown in fig. 17, the storage rack 321 is used for storing the freezing tubes 22, the lifting mechanism 322 can drive the storage rack 321 to move, the storage rack 321 can be moved into or out of the freezing chamber 323 in the process of ascending or descending of the lifting mechanism 322, and the refrigerating element 324 can play a role in refrigerating the freezing chamber 323, so that the internal temperature of the freezing chamber 323 is always maintained at-196 ℃, and further the freezing tubes 22 at-80 ℃ can be cooled to-196 ℃. It should be noted that the lifting mechanism 322 may be any moving component that can be used for position transfer, such as a pneumatic cylinder, a hydraulic cylinder, an electric push rod, a conveying guide rail 441, and the like, and the working principle of the cooling element 324 includes, but is not limited to, cooling by a semiconductor cooling plate, cooling by slow evaporation and heat absorption by liquid nitrogen, cooling by heat exchange by a heat engine, and the like.

In other embodiments, in order to transfer the material inside the temperature programmed module 300 or to other modules, a transfer mechanism 340 may be further installed in the housing 500 provided with the temperature programmed module 300, and the transfer mechanism 340 may move laterally or longitudinally.

The consumable management module 400 includes a rack 410 for disposing consumables, a clamping assembly 420 for transferring the consumables, and an identification mechanism, wherein the identification mechanism includes a first sensor 431 disposed toward the rack 410 for detecting a position of the consumables disposed on the rack 410, and an identification member disposed on the clamping assembly 420 for identifying a type of the consumables.

It should be noted that a plurality of the carriers 410 may be provided as required, and the carriers 410 may be set to rotate or be stationary, and may be driven by a motor when rotating; the consumables comprise articles to be used in the culture process such as a centrifuge tube 110, a pipette tip 111 and a disposable counting plate; the identification piece can be a wharf scanning head and is used for scanning a two-dimensional code or a one-dimensional code on the consumable; the clamping assembly 420 may rotate the consumables from the rack 410 out of the consumable management module 400, and allocate the consumables to the consumable storage device 150, or rotate the consumables from other modules except the consumable management module 400 to the rack 410.

It can be understood that, the consumable material needed is installed in the consumable material management module 400, the consumable material is allocated to the consumable material storage device 150 by the consumable material management module 400, as shown in fig. 18-19, the consumable material is stored by the carrier 410, the consumable material storage device 150 can be continuously supplied, the automatic allocation and transfer of the consumable material is realized by the clamping component 420 and the identification mechanism, and the consumable material identification management function is also provided, when a type of consumable material needs to be allocated, the consumable material needed is identified from the carrier 410 by the first sensor 431 and the identification component, and the corresponding consumable material is transferred to the target position by the clamping component, because the consumable material is arranged on the carrier 410, the temporary allocation when needed is not needed, the condition of insufficient consumable material is preferably avoided, the normal operation of cell culture can be effectively ensured, and simultaneously the first sensor 431, the identification component and the clamping component 420 work together, the accuracy of consumable material preparation is improved, the operation time increased due to errors of personnel preparation is reduced, and the time for consumable material preparation is greatly saved.

In one embodiment, the biological tissue production system includes an environmental control mechanism 600 and a plurality of housings 500, the functional module group is provided in plurality, each module is installed in each housing 500 in a one-to-one correspondence manner, the environmental control mechanism 600 includes a control box 601 installed at the top of the housing 500, a first primary filter 602 installed in the control box 601 and communicating with the outside, a first centrifugal fan 603 installed in the control box 601, a first high efficiency filter 604 communicating with the inside of the housing 500, and a first sensor group 605 installed on the control box 601. It can be understood that, as shown in fig. 20, the working environment inside each housing 500 can be regulated in real time, external air enters the regulation and control box 601 after being primarily filtered by the first primary filter 602, the first centrifugal fan 603 can form an air flow to drive the air to flow when operating, so as to provide a driving force for air circulation, and air inside the regulation and control box 601 can flow into the housing 500 after being secondarily filtered by the first high efficiency filter 604 under the driving of the first centrifugal fan 603; the first high efficiency filter 604 can effectively improve the cleanliness of the air entering the casing 500, and on the other hand, the air can form parallel air flow from top to bottom under the action of the first centrifugal fan 603.

In order to facilitate the constant temperature and humidity regulation of the air in the regulation and control box 601, a first temperature and humidity controller 606 is installed inside the regulation and control box 601, in order to perform disinfection and sterilization treatment on the internal environment of the casing 500, a first sterilization actuator 607 is installed inside the regulation and control box 601, external air can enter the regulation and control box 601 after being primarily filtered by a first primary filter 602, the first temperature and humidity controller 606 performs constant temperature and humidity treatment on the filtered air, and the first sterilization actuator 607 can rapidly generate ozone gas or hydrogen oxide vapor so as to be mixed with the air after the constant temperature and humidity treatment when the inside of the casing 500 needs to be disinfected and sterilized and then flow into the inside of the casing 500 after being secondarily filtered by a first high efficiency filter 604.

First sensor group 605 can the inside environmental condition of real-time supervision casing 500, and first sensor group 605 at least part stretches into inside casing 500 to feed back to first atmospheric control ware 606 department when surveying the inside environmental condition of casing 500 in real time, in order to guarantee the accuracy to the inside environmental control of casing 500. Preferably, the first sensor group 605 may be selected from one or more of a wind speed sensor, a humidity sensor, a temperature sensor, an ozone concentration sensor, and a hydrogen peroxide concentration sensor.

In one embodiment, the biological tissue production system further comprises a control module 700 electrically connected to the functional module set, the control module 700 comprises a positioning column 701, an electric control cabinet 702 fixed on the positioning column, a torsion shaft 704 rotatably disposed on the positioning column 701, and a control panel 703 fixedly connected to the torsion shaft 704, it can be understood that, as shown in fig. 21, the control module 700 can be mechanically connected to the functional module set through a fixing interface 910 disposed on the positioning column 701, the control module 700 can be electrically connected to the functional module set through the control interface 900, the electric control cabinet 702 is used for controlling the operation of the biological tissue production system, the control panel 703 is a user interaction interface, the control panel 703 has a touch screen 705, an alarm 706, a signal lamp 707, and an emergency stop switch 708, as described in the prior art, the torsion shaft 704 allows the control panel 703 to freely rotate relative to the positioning post 701, so as to adjust the angle of use.

The culture operation module 100 and the culture module 200 are controlled by their internal controllers, and the controllers in the culture operation module 100 or the culture module 200 may also control other modules, or the operation and control module 700 may collectively manage and operate the modules.

In one embodiment, the biological tissue production system further includes an external environment control module 800, as shown in fig. 1, the external environment control module 800 includes an air casing 801, a partition plate 802, a second primary filter 803, a second centrifugal fan 804, a second temperature and humidity controller 805, a second sterilization actuator 806, a second sensor group 807, an air supply pipeline 808, and a second high efficiency filter 809, the partition plate 802 divides the air casing 801 into an upper air pressure cabin and a lower air inlet cabin; the second primary filter 803 is vertically installed on the side face of the wind shell 801, the second sensor group 807 is located at a position where the pressure cavity leads to the air supply pipeline 808, and is used for measuring the environment inside the equipment and feeding back to the external environment control module 800 in real time to ensure the accuracy of internal environment control of the biological tissue production system, the air supply pipeline 808 is connected with the pressure cavity and each module, clean high-pressure air flow processed by the external environment control module 800 is conveyed to each module through the air supply pipeline 808, the second high-efficiency filter 809 is located at the tail end and the top of each module reached by the air supply pipeline 808, namely, the clean high-pressure air flow conveyed to each module by the air supply pipeline 808 is finally filtered by the second high-efficiency filter 809 to form vertically downward parallel air flow meeting the cleanliness requirement.

It should be noted that the working principle of the second centrifugal fan 804, the second primary filter 803, the second temperature and humidity controller 805 and the second sterilization actuator 806 can refer to the environment control mechanism 600; the external environmental control module 800 is additionally provided for the biological tissue production system, and can be used in cooperation with the culture operation module 100, the culture module 200, the program cooling module 300 and the consumable management module 400, so that all functions of the environmental control built in each module can be realized.

In summary, in the embodiment of the present invention, at least one culture operation module 100, at least one culture module 200, at least one program cooling module 300, and at least one consumable management module 400 are connected to each other through the fixed interface 910 and the control interface 900 according to the culture conditions of the cells, in addition, the external environment control module 800 may be additionally installed according to the actual needs to cooperate with the environment control mechanism 600 in each module to sterilize and disinfect the interior of each corresponding module, and after sterilization is completed, the environment control mechanism 600 performs temperature and humidity control on the interior of each corresponding module; or the external environment control module 800 can perform sterilization and temperature and humidity control operations, and the operation logic and operation program can be set in the control module 700 according to the cell types to be cultured and the predicted output cell amount, and the consumable material management module 400 can supply various required consumable materials in the operation process of the biological tissue production system.

The present invention also provides a method of producing a biological tissue production system for use in the biological tissue production system, the biological tissue production system continuing to refer to fig. 1-21, the method comprising the steps of:

judging whether the cells to be amplified are adherent cells in the culture bottle 30 or cells in a frozen state in the freezing storage tube 22, if the cells are adherent cells, judging whether the growth state of the adherent cells in the culture bottle 30 meets the passage requirement by the microscope 171, if the growth state meets the passage requirement, performing passage operation, pouring the culture medium discarded in the culture bottle 30 into a device of liquid waste by the clamping mechanism 50, transferring the culture bottle 30 to the liquid treatment device 160 by the clamping mechanism 50, filling digestive enzymes which are recovered to the normal temperature in the refrigerator 170 by the liquid treatment device 160 into the culture bottle 30, transferring the culture bottle 30 filled with the digestive enzymes into the culture module 200 through the combined action of the clamping mechanism 50 and the manipulator 230 for incubation for a preset time, then moving out the culture box, transferring the culture bottle 30 to the liquid treatment device 160 by the clamping mechanism 50, filling the culture medium which is recovered to the normal temperature in the refrigerator 170 by the liquid treatment device 160 into the culture bottle 30, the cell after digestion by the digestive enzymes in the culture flask 30 is separated from the wall surface by the sucking and discharging action of the pipetting mechanism 70 and the pipette tip 111 obtained from the consumable storage device 150 or the shaking of the clamping mechanism 50, so as to form a cell suspension, and the consumable used in the culture operation process is poured into the device for solid waste.

Or transferring the cell sap in the culture storage mechanism into the centrifuge tube 110, and transferring the centrifuge tube 110 onto the centrifuge 172 by the clamping mechanism 50 for centrifugal treatment to obtain centrifugal cell sap; the supernatant of the centrifuged cell liquid is poured into a device for liquid waste, the clamping mechanism 50 moves the centrifuge tube 110 to the liquid processing device 160, the culture medium in the refrigerator 170, which is returned to normal temperature by the liquid processing device 160, is filled into the centrifuge tube 110, the centrifuge tube 110 obtains cell suspension through the sucking and spitting action of the liquid transfer mechanism 70 and the liquid transfer gun head 111 or the shaking of the clamping mechanism 50, the density value of the cell suspension is detected, the cell suspension with a preset amount can be moved from the centrifuge tube 110 through the liquid transfer gun head 111 to a counter (see the prior art) according to the detected density value, the density value of the cell suspension is detected and recorded by the counter, and consumables used in the process of culture operation are poured into the device for solid waste.

Judging a preset state that cells need to be output, if adherent cells need to be output, inoculating cell suspension in a centrifugal tube 110 into a plurality of new culture bottles 30 according to the density value, filling culture medium into the culture bottles 30 by a liquid processing device 160, transferring the culture bottles 30 into a culture module 200 for culture by a clamping mechanism 50 and a manipulator 230, judging whether the growth state of the cells in the culture bottles 30 meets the passage requirement or not by a microscope 171 in a culture operation module 100 after each preset time, and if the growth state of the cells meets the passage requirement, transferring the adherent cells out of the biological tissue production system by the culture operation module 100; if the preset state to be output is the cells in the frozen state, the supernatant of the centrifuged cell sap is poured in the above steps, the frozen stock solution is filled into the centrifuge tube 110 through the liquid processing device 160, the cell suspension is formed in the centrifugal tube 110 by the sucking and discharging action of the liquid-transferring mechanism 70 and the liquid-transferring gun head 111, and the density value of the cell suspension is obtained by detecting, referring to the above-mentioned detection operation, seeding the cell suspension in the centrifuge tube 110 into a plurality of new cryopreservation tubes 22 according to the density value, the freezing pipe 22 is transferred to the program cooling module 300 for cooling through the combined action of the batch grabbing mechanism 60 and the transferring mechanism 340, and after a preset time, the freezing tube 22 is transferred to the outside of the biological tissue production system through the culture operation module 100, and the freezing tube 22 is transferred to the outside of the biological tissue production system and can be transferred by the batch grabbing mechanism 60 or stored in the freezing chamber 323 of the ultra-low temperature storage mechanism 320.

If the cells to be amplified are cells in a frozen state in the cryopreservation tube 22, the cryopreservation tube 22 is transferred to the thawing and uncapping device 120 by the batch grabbing mechanism 60, the cryopreservation tube 22 is thawed and uncapped, the centrifuge tube 110 is taken out of the consumable storage device 150 by the clamping mechanism 50, cell strains in the cryopreservation tube 22 can be transferred into the centrifuge tube 110 by the pipetting mechanism 70 and the pipetting gun head 111, and centrifugal treatment is carried out by the centrifuge 172 to obtain centrifugal cell liquid; obtaining the cell suspension of the centrifuge tube 110, detecting and recording the density value of the cell suspension, referring to the above operation process to be expanded into adherent cells, determining the preset state of the cells to be output, and outputting the cells in the frozen state, or the adherent cells, referring to the above steps.

It should be noted that, if the adherent cells to be expanded do not meet the passage requirement, the culture bottle 30 inoculated with the cell strain to be expanded is directly sent into the culture module 200 for continuous culture, after every preset time, the microscope 171 determines whether the cells in the culture bottle 30 meet the passage requirement and simultaneously displays whether the culture medium needs to be replaced, if the cells need to be replaced, the clamping mechanism 50 dumps the waste culture medium in the culture bottle 30 into the liquid waste device, then transfers the culture bottle 30 to the liquid treatment device 160, fills the culture medium in the refrigerator 170, which is restored to the normal temperature by the liquid treatment device 160, into the culture bottle 30, and the consumables used in the culture operation process are dumped into the solid waste device.

The method for producing the biological tissue production system further comprises the steps of combining the functional module groups before obtaining the centrifugal cell sap, installing consumables to be used in the consumable management module 400, allocating the consumables to the consumable storage device 150 by the consumable management module 400, and supplying the consumables to the biological tissue production system continuously by the consumable management module 400.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. A biological tissue production system, comprising: comprises at least one culture operation module (100), at least one culture module (200), at least one program cooling module (300) and at least one consumable management module (400); all modules are mechanically connected through a fixed interface (910) and electrically connected through a control interface (900); the culture operation module (100) is used for simulating manual operation to realize automation of cell culture; the culture module (200) is used for culturing cells; the program cooling module (300) performs program cooling and low-temperature storage on the sample needing to be frozen; the consumable management module (400) provides various required consumables for operation of the bio-tissue production system.

2. Biological tissue production system according to claim 1, wherein the culture operation module (100) comprises: a culture storage mechanism (10) for inoculating cell strains, a thawing and uncapping device (120) for thawing and uncapping the culture storage mechanism (10) which is frozen, a consumable storage device (150) provided with a centrifuge tube (110), a clamping and moving device for clamping the centrifuge tube (110) and/or the culture storage mechanism (10), and a liquid processing device (160) for processing and transferring reagents; the clamping and moving device comprises a batch grabbing mechanism (60), a clamping mechanism (50) and a liquid transferring mechanism (70), wherein the batch grabbing mechanism (60) can grab the cryopreservation assembly (20) of the culture and storage mechanism (10), and the clamping mechanism (50) operates a culture bottle (30) and a centrifuge tube (110) of the culture and storage mechanism (10); the unfreezing and cover opening device (120) is matched with the batch grabbing mechanism (60) to unfreeze the cryopreservation assembly (20).

3. The biological tissue production system according to claim 1, wherein the culture module (200) comprises: the culture box comprises a culture box body, a rotary culture disc (210) at least partially arranged in the culture box body, an automatic door (220) positioned on the culture box body and a manipulator (230) positioned outside the automatic door (220); the culture box body is provided with a culture cavity (204) and a first accommodating cavity (205) which is arranged at an interval with the culture cavity (204), a sensor component (201) is arranged in the first accommodating cavity (205), one end of a first air duct (202) is communicated with the culture cavity (204), the other end of the first air duct is communicated with the first accommodating cavity (205), one end of a second air duct (203) is communicated with the first accommodating cavity (205), and the other end of the second air duct is communicated with the culture cavity (204).

4. The biological tissue production system according to claim 1, wherein the program cooling module (300) comprises: a programmed cooling mechanism (310) and an ultra-low temperature storage mechanism (320) for cooling the cryopreserved sample; the program cooling mechanism (310) is used for realizing sequential step cooling by transferring the temperature guide blocks (312) between any two adjacent constant-temperature freezing blocks (311) through the circulating motion of the circulating mechanism (330) and the temperature guide blocks (312) relative to the constant-temperature freezing blocks (311), and flexibly adding a new sample to be frozen into the freezing well of the temperature guide blocks (312) while performing step cooling, or placing the sample to be frozen into the temperature guide blocks (312) with corresponding set temperature according to the initial temperature of the newly added sample to be frozen; the ultra-low temperature storage mechanism (320) is used for storing the cryopreserved sample cooled by the program cooling mechanism (310).

5. The biological tissue production system according to claim 1, wherein the consumable management module (400) comprises: a carrier rack (410) for arranging consumables, a clamping assembly (420) for transferring the consumables; the object carrier (410) is arranged in a rotating or static way, the object carrier (410) is driven by a motor when rotating, and in the rotating process of the object carrier (410), the residual space on the saddle (411) is locked for installing and loading objects (412); when a type of consumable is required to be prepared, the first sensor (431) identifies the installation condition of the object carrying piece (412), the identification piece on the clamping assembly (420) identifies the consumable, and the consumable is transferred to a target position by the clamping piece (424) after the identification piece identifies the consumable.

6. The biological tissue production system according to claim 1, further comprising a housing (500) in which the modules are installed in a one-to-one correspondence, and an environment control means (600) in which the environment control means (600) is embedded and installed; the environment control mechanism (600) comprises a regulation and control box (601) arranged at the top of the shell (500), a first primary filter (602) arranged in the regulation and control box (601) and communicated with the outside, a first centrifugal fan (603) arranged in the regulation and control box (601), a first high-efficiency filter (604) communicated with the inside of the shell (500), a first temperature and humidity controller (606), a first sterilization actuator (607) and a first sensor group (605) arranged on the regulation and control box (601).

7. The biological tissue production system according to claim 1, further comprising a manipulation module (700), wherein the manipulation module (700) is mechanically connected to each of the modules through a fixed interface (910) and electrically connected to each of the modules through the control interface (900); the control module (700) comprises a positioning column (701), an electric control cabinet (702) fixed on the positioning column (701), a torsion shaft (704) rotatably arranged on the positioning column (701), and a control panel (703) fixedly connected with the torsion shaft (704).

8. The biological tissue production system according to claim 1, further comprising an external environment control module (800), wherein the external environment control module (800) comprises a wind shell (801), a separation plate (802), a second primary filter (803), a second centrifugal fan (804), a second temperature and humidity controller (805), a second sterilization actuator (806), a second sensor group (807), a wind supply pipeline (808), and a second high efficiency filter (809); the separation plate (802) is separated wind shell (801) for the wind pressure storehouse of top and the air inlet storehouse of below, the side at wind shell (801) is installed perpendicularly to second primary filter (803), second sensor group (807) be located the position that the pressure chamber leads to supply air duct (808), supply air duct (808) connect pressure chamber and each module to carry each module through supply air duct (808) with the clean high-pressure draught that has handled through external environmental control module (800), second high efficiency filter (809) be located supply air duct (808) and reach the end and the top of each module.

9. A method for producing a biological tissue production system, which is used for the biological tissue production system according to any one of claims 1 to 8, comprising the steps of:

s1: according to the cell culture conditions in the culture storage mechanism (10), at least one culture operation module (100), at least one culture module (200), at least one program cooling module (300), at least one consumable management module (400) and one control module (700) are connected with one another through respective fixed interfaces (910) and control interfaces (900) to form a set of complete biological tissue production system;

s2: starting respective environment control mechanisms (600) of the culture operation module (100), the culture module (200), the program cooling module (300) and the consumable management module (400), sterilizing the biological tissue production system, and starting temperature and humidity control of the environment control mechanisms (600) after sterilization is finished;

s3: spraying or pasting two-dimensional codes/one-dimensional codes on consumables to be used in the consumable management module (400), and setting operation logic and operation programs of the biological tissue production system in the control module (700) according to the cell types to be cultured and the predicted output cell amount;

s4: the cell strain for culture is a cell cultured in a culture bottle (30) in an adherent way, a frozen cell stored in a freezing tube (22) or a cell preserved in equipment; the cells are accessed from the outside of the biological tissue production system to the inside thereof through a culture operation module (100); transferring the cell sap in the culture storage mechanism (10) into a centrifuge tube (110) for centrifugal treatment to obtain centrifugal cell sap; pouring the supernatant of the centrifuged cell sap, and filling a reagent into the centrifuge tube (110) for processing to obtain a cell suspension; detecting to obtain a density value of the cell suspension, inoculating the cell suspension into a new culture storage mechanism (10) according to the density value, and judging a preset state of the cells to be output; if the output of the frozen cells is preset, transferring the new culture storage mechanism (10) to a program cooling module (300) for cooling, then transferring to an ultralow temperature storage mechanism (320) for ultralow temperature preservation treatment, and transferring the frozen cells to the outside of the biological tissue production system through a culture operation module (100) after a preset time; if the adherent cells are preset to be output, transferring the new culture storage mechanism (10) to a culture module (200) for cell culture, and judging whether the growth state of the cells meets the passage requirement after the cells are cultured for a preset time; and if the cultured cells meet the passage requirement, passage operation is carried out on the adherent cells through a culture operation module (100) or the adherent cells are transferred out of the biological tissue production system.

10. The method of claim 9, wherein in S1, an external environmental control module (800) may be added as required; in S3, the consumables comprise a culture bottle (30), a centrifuge tube (110), a freezing tube (22) and a pipette tip box; in S4, the cell culture condition can be observed in real time by using a microscope (171).