CN215668049U

CN215668049U - Biological tissue production system

Info

Publication number: CN215668049U
Application number: CN202121239815.7U
Authority: CN
Inventors: 倪孝杰; 赖雪聪; 徐铭恩
Original assignee: Regenovo Biotechnology Co ltd
Current assignee: Regenovo Biotechnology Co ltd
Priority date: 2021-06-03
Filing date: 2021-06-03
Publication date: 2022-01-28
Anticipated expiration: 2031-06-03

Abstract

The present invention relates to a biological tissue production system comprising: each functional module group comprises at least one culture operation module and at least one culture module which is connected with and communicated with the culture operation module; the culture operation module comprises a culture storage mechanism for inoculating cell strains, a thawing and cover opening device for thawing and opening the frozen culture storage mechanism, a consumable storage device for installing consumables, a clamping and moving device for clamping the consumables and/or the culture storage mechanism, and a liquid processing device for processing and transferring reagents; the culture module comprises a culture box body and a rotary culture disc at least partially arranged in the culture box body. The technical scheme of the utility model can freely combine the culture operation module with more complete automatic operation conditions and the culture module with better culture environment according to the application requirements, thereby meeting the complex cell production requirements.

Description

Biological tissue production system

Technical Field

The utility model relates to the field of biological tissue production, in particular to a biological tissue production system.

Background

With the continuous development of laboratory equipment, some automated equipment for cell expansion appears, but the equipment can only be applied to specific several cell expansion modes due to a fixed mode, and is difficult to meet the increasingly complex cell production requirement.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defect that the prior art is difficult to meet the requirement of complex cell production, and provides a biological tissue production system.

In order to achieve the purpose, the utility model adopts the following technical scheme:

a biological tissue production system comprising: each functional module group comprises at least one culture operation module and at least one culture module which is connected with and communicated with the culture operation module;

the culture operation module comprises a culture storage mechanism for inoculating cell strains, a thawing and cover opening device for thawing and opening the cover of the culture storage mechanism which is frozen and stored, a consumable storage device for installing consumables, a clamping and moving device for clamping the consumables and/or the culture storage mechanism, and a liquid processing device for processing and transferring reagents; the culture module comprises a culture box body, a rotary culture disc at least partially arranged in the culture box body, an automatic door (220) arranged on the culture box body, and the mechanical arm (230) arranged at an interval with the culture box body;

wherein, press from both sides the device of moving will centrifuging tube on the consumptive material storage device moves to the target location to with freezing the cultivation storage mechanism shifts to unfreeze uncapping device, the warp unfreeze uncapping device and unfreeze and resume to the normal atmospheric temperature and open cultivation storage mechanism will the cell sap in the cultivation storage mechanism shifts to carry out centrifugal treatment in the centrifuging tube, again through partial shipment inoculation to new cultivation storage mechanism after, by press from both sides the device of moving and shift to in the cultivation box body and set up in carry out cell culture on the rotatory cultivation dish, the cultivation is accomplished the back warp cultivation operation module shifts to outside the biological tissue production system.

Preferably, press from both sides and move the device including being used for the centre gripping centrifuge tube and/or cultivate the fixture of storage mechanism, fixture includes first driving piece, and is fixed in a plurality of centre gripping groups on the first driving piece, each the centre gripping group presss from both sides and locates centrifuge tube or cultivate on the surface of storage mechanism, each the centre gripping group includes along first driving piece side is a plurality of holders that encircle the setting.

Preferably, press from both sides and move the device and still grab the mechanism that moves including batch, grab in batches and move the mechanism and include the base shell, articulate in grab on the base shell and install in the pipe fitting of grabbing of base shell bottom surface, cultivate storage mechanism including freezing the subassembly, freeze the subassembly including freezing the shell and install in freeze the pipe of depositing in the shell, grab the piece and keep away from one side of base shell press from both sides tightly in the surface of freezing the shell, grab the pipe fitting and keep away from one side of base shell is inserted and is established and be fixed in freeze in the pipe.

Preferably, the batch grabbing and moving mechanism further comprises a cylinder arranged on one side of the grabbing pipe fitting far away from the base shell, a moving member fixedly connected with the output end of the cylinder and arranged in the base shell, and a pushing member fixed on one side of the moving member far away from the cylinder and arranged in the grabbing pipe fitting in a penetrating mode, wherein the moving member and the grabbing member are arranged in a sliding mode.

Preferably, the consumable storage device further comprises a carrier, and at least one positioning rack mounted on the carrier.

Preferably, the culture operation module further comprises a pipetting head which is arranged on the carrier frame and used for transferring liquid, and the clamping and moving device further comprises a pipetting mechanism, wherein the pipetting head is arranged on the pipetting mechanism.

Preferably, cultivate the operation module still include and shift to reagent the blake bottle or liquid processing apparatus in the centrifuging tube, liquid processing apparatus includes the liquid transfer pipeline, all install in heat pack and the pump body on the liquid transfer pipeline, and with the appearance piece that adds that liquid transfer pipeline one end intercommunication set up, cultivate storage mechanism still including the blake bottle that the inoculation has the cell, liquid transfer pipeline the other end with the blake bottle or centrifuging tube intercommunication sets up.

Preferably, the culture module further comprises a sensor assembly, a first air duct and a second air duct, the culture box body is provided with a culture cavity and a first accommodating cavity arranged at an interval with the culture cavity, the sensor assembly is arranged in the first accommodating cavity, the first air duct penetrates through the side wall of the culture box body, one end of the first air duct is communicated with the culture cavity, the other end of the first air duct is communicated with the first accommodating cavity, one end of the second air duct is communicated with the first accommodating cavity, and the other end of the second air duct is communicated with the culture cavity; the rotary culture disc comprises a rotary seat, a rotary motor, a rotary disc and at least one culture frame, wherein part of the rotary seat, the rotary motor, the rotary disc and the at least one culture frame penetrate into the culture cavity, the rotary motor is arranged on the rotary seat and is in driving connection with the rotary disc, each culture frame is detachably arranged on one side, back to the rotary seat, of the rotary disc, the rotary motor is positioned outside the culture box body, and the rotary disc is positioned in the culture cavity.

Preferably, the biological tissue production system further comprises an environment control mechanism and a plurality of shells, wherein the functional module group is provided with a plurality of shells, each culture operation module or each culture module is installed in each shell in a one-to-one correspondence manner, the environment control mechanism comprises a regulation and control box installed at the top of each shell, a first primary filter installed in the regulation and control box and communicated with the outside, a first centrifugal fan installed in the regulation and control box, a first high-efficiency filter communicated with the inside of each shell, and a first sensor group installed on the regulation and control box; biological tissue production system still includes external environmental controller, external environmental controller includes the wind shell, is used for separating the division board of wind shell, install in the first filter of second of wind shell side set up in second centrifugal fan on the division board, install in second atmospheric control ware and second sterilization executor in the wind shell, with the supply air duct that the inside intercommunication of wind shell set up, install in just be close to in the wind shell the second sensor group of supply air duct tip to and set up on the surface of each module and with the second high efficiency filter that the supply air duct connection set up.

Preferably, the biological tissue production system further comprises an operation module electrically connected to the functional module group, wherein the operation module comprises a positioning column, an electric control cabinet fixed on the positioning column, a torsion shaft rotatably disposed on the positioning column, and an operation panel fixedly connected to the torsion shaft.

Compared with the prior art, the utility model has the beneficial effects that:

according to the biological tissue production system provided by the technical scheme, each functional module group comprises at least one culture operation module and at least one culture module which is connected with the culture operation module and is communicated with the culture operation module, and based on the clamping and moving device, the unfreezing and cover opening device and the liquid processor in the culture operation module, multiple operations can be better performed on consumables and a culture storage mechanism instead of manual work, so that the operation efficiency is improved, consumables required by the consumables storage device are stored, the smooth operation is better ensured, the participation of personnel in the cell culture process is reduced, and the production automation is improved; arranging a culture box body and a rotary culture disc, and providing a culture environment and a set position for a culture storage mechanism needing to be cultured; each functional module group has relatively complete automatic operation conditions and capacity and relatively good culture environment, so that each functional module group can be closed and independently operated, the number of culture operation modules and culture modules in each functional module group can be set according to needs, each culture operation module and each culture module can be freely combined according to application requirements, and the cell culture system has extremely high flexibility and expansibility and can meet the complex cell production requirements.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and other drawings can be obtained by those skilled in the art without creative efforts.

FIG. 1 is a front view of a biological tissue production system of the present invention;

FIG. 2 is a top view of a biological tissue production system of the present invention;

FIG. 3 is a schematic view showing the internal structure of the culture operation module shown in FIG. 1;

FIG. 4 is a schematic view of the clamping mechanism and the culture bottle according to the embodiment of the present invention;

FIG. 5 is an exploded view of the clamping mechanism, the vial and the centrifuge tube according to an embodiment of the present invention;

FIG. 6 is a schematic structural diagram of a batch grabbing and moving mechanism and a freezing shell in the embodiment of the utility model;

FIG. 7 is a schematic structural view of the batch grabbing and moving mechanism shown in FIG. 6;

FIG. 8 is a schematic structural diagram of the consumable storage device shown in FIG. 3;

FIG. 9 is a schematic structural view of the pipetting mechanism shown in FIG. 3;

FIG. 10 is a schematic view of a liquid treatment apparatus according to an embodiment of the present invention;

FIG. 11 is a schematic view showing the internal structure of the culture module;

FIG. 12 is a schematic view showing the structure of a rotary culture tray;

FIG. 13 is a schematic view of the structure of the culture module;

FIG. 14 is a schematic view of an environmental control mechanism in an embodiment of the present invention;

FIG. 15 is a schematic structural diagram of the control module shown in FIG. 2;

description of reference numerals:

100. a culture operation module; 21. freezing and storing the shells; 22. freezing and storing the tube; 30. a culture bottle; 41. a mechanical arm; 50. a clamping mechanism; 51. a first driving member; 52. a clamping group; 53. a clamping member; 60. a batch grabbing and moving mechanism; 61. a base shell; 62. a grasping member; 63. grabbing the pipe fitting; 64. a cylinder; 70. a pipetting mechanism; 110. centrifuging the tube; 111. a pipette tip; 112. a consumable housing; 120. a cover opening device is unfrozen; 150. a consumable storage device; 151. a carrier; 152. a positioning frame; 160. a liquid treatment device; 161. a liquid transfer pipeline; 162. a heating assembly; 163. a pump body; 164. adding a sample piece; 170. a refrigerator; 171. a microscope; 172. a centrifuge;

200. a culture module; 201. a sensor assembly; 202. a first gas guide pipe; 203. a second gas guide pipe; 204. culturing the cavity; 205. a first accommodating cavity; 210. rotating the culture tray; 211. a rotating base; 212. a rotating electric machine; 213. rotating the disc; 214. a culture shelf; 220. an automatic door; 230. a manipulator;

300. a housing; 400. an environmental control mechanism; 401. a regulatory cassette; 402. a first primary filter; 403. a first centrifugal fan; 404. a first high efficiency filter; 405. a first sensor group; 406. a first temperature and humidity controller; 407. a first sterilization actuator;

500. a control module; 501. a positioning column; 502. an electric control cabinet; 503. a control panel; 504. a torsion shaft; 505. a touch screen; 506. an alarm; 507. a signal lamp; 508. a scram switch;

600. an external environment controller; 601. a wind shell; 602. a partition plate; 603. a second primary filter; 604. a second centrifugal fan; 605. a second temperature and humidity controller; 606. a second sterilization actuator; 607. a second sensor group; 608. an air supply duct; 609. a second high efficiency filter; 700. a control interface; 710. and fixing the interface.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings, and it should be understood that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it should be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection, a removable connection, or an integral connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 15, an embodiment of the present invention provides a biological tissue production system, including: at least one functional module group, wherein each functional module group comprises at least one culture operation module 100 and at least one culture module 200 connected with and communicated with the culture operation module 100.

The culture operation module 100 and the culture module 200 are respectively provided with a shell 300, the side surface of each shell 300 is provided with a fixed interface 710 and a control interface 700, the culture operation module 100 is fixedly connected with the culture module 200 through the fixed interface 710, the control interface 700 is combined with or separated from the fixed interface 710, the control interface 700 is used for electrically connecting control lines of each module, and the fixed interface 710 can adopt the modes of screw locking, pin and pin hole locking, electromagnetic lock locking, air lock locking and the like.

The culture operation module 100 is a main action part of each functional module group, simulates manual work to perform various operations, the culture module 200 is a place for cell growth and differentiation, each functional module group has relatively complete automatic operation conditions and capabilities, and also has a relatively good culture environment, so that each functional module group can be sealed and independently operated, the number of the culture operation modules 100 and the culture modules 200 in each functional module group can be set as required, each culture operation module 100 and each culture module 200 can be freely combined according to application requirements, and the culture operation module has extremely strong flexibility and expansibility, and meets the requirement of complex cell production.

The culture operation module 100 includes a thawing and uncapping device 120 for thawing and uncapping the culture storage mechanism frozen and stored by the culture storage mechanism for inoculating the cell strain, a consumable storage device 150 for installing consumables, a gripping and moving device for gripping the consumables and/or the culture storage mechanism, and a liquid processing device 160 for processing and transferring the reagent.

It should be noted that, it can be used to centre gripping centrifuging tube 110 or cultivate storage mechanism etc. to press from both sides the device of moving, cultivate storage mechanism can be conveyed inside casing 300 by the outside in process of production, also can set up inside casing 300 before producing, cultivate storage mechanism and treat that the cell strain of expanding can be for inoculating to the adherent cell of blake bottle 30, also can be for freezing the cell that is in frozen state in the tube 22 that freezes, and new cultivation storage mechanism is empty blake bottle 30, and obtains from consumptive material storage device 150 by pressing from both sides the device of moving, cultivates storage mechanism business turn over biological tissue production system can be accomplished by pressing from both sides the device of moving, of course also can set up other transport mechanism.

It can be understood that, the installation still have arm 41 on cultivateing operating module 100's the casing 300, arm 41 with fixture 50 combined action, replacement manual work that can be better carries out multiple operation, improves the operating efficiency to utilize consumptive material storage device 150 to save consumptive materials such as centrifuging tube 110 that need use, avoid taking place because of the consumptive material lacks the problem that the termination operation goes on, and can reduce cell culture in-process personnel's participation, improve production automation.

The clamping and moving device includes a clamping mechanism 50 for clamping the centrifuge tube 110 and/or the culture storage mechanism, the clamping mechanism 50 includes a first driving member 51, and a plurality of clamping groups 52 fixed on the first driving member 51, each clamping group 52 is clamped on the outer surface of the centrifuge tube 110 or the culture storage mechanism, each clamping group 52 includes a plurality of clamping members 53 disposed around the side of the first driving member 51, it can be understood that the clamping mechanism 50 can be used for transferring articles between the culture operation module 100 and other modules, as shown in fig. 4-5, the first driving member 51 drives the clamping groups 52, so that the clamping members 53 in each clamping group 52 move towards or away from each other, thereby opening or closing the clamping groups 52 is realized, the number, size, shape and the like of the clamping groups 52 can be adjusted as required, each of the clamping groups 52 may clamp a centrifuge tube 110 or a culture flask 30 or a vial 22, etc.

In one embodiment, the cell strain to be expanded is a cell in a frozen state in the freezing tube 22, in order to realize the clamping and transferring of the freezing tube 22, the culture and storage mechanism comprises a freezing assembly, the freezing assembly comprises a freezing shell 21 and the freezing tube 22 installed in the freezing shell 21, the clamping and transferring device further comprises a batch clamping and transferring mechanism 60, the batch clamping and transferring mechanism 60 comprises a base shell 61, a clamping piece 62 hinged to the base shell 61, and a clamping tube piece 63 installed on the bottom surface of the base shell 61, one side of the clamping piece 62, which is far away from the base shell 61, is clamped on the outer surface of the freezing shell 21, and one side of the clamping tube piece 63, which is far away from the base shell 61, is inserted and fixed in the freezing tube 22.

It can be understood that one or more freezing tubes 22 can be stored in the freezing shell 21, as shown in fig. 6-7, since the gripping member 62 is hinged to the base shell 61, the gripping member 62 can rotate relative to the base shell 61, not only clamping or releasing the freezing shell 21 can be realized, but also the freezing shells 21 with different sizes can be clamped in an adaptive manner, and the gripping member 62 has better applicability, and when the freezing tubes 22 in the freezing shell 21 need to be transferred, the gripping member 63 can be fixed in an interference fit manner with the freezing tubes 22 under the action of the mechanical arm 41, and the freezing tubes 22 fixed on the gripping member 63 can be transferred to a target position.

In order to take down the frozen pipe 22 fixed on the grabbing pipe 63, the batch grabbing and moving mechanism 60 further comprises a cylinder 64 arranged on one side of the base shell 61 far away from the grabbing pipe 63, a moving member fixedly connected with the output end of the cylinder 64 and arranged in the base shell 61, and a pushing member fixed on one side of the moving member far away from the cylinder 64 and arranged in the grabbing pipe 63 in a penetrating manner, wherein the moving member and the grabbing member 62 are arranged in a relative sliding manner.

It can be understood that, in this embodiment, the air cylinder 64 is used for driving the moving member to move up and down relative to the base shell 61, the pushing member can move axially along the gripping pipe member 63 along with the moving member, when the frozen pipe 22 needs to be taken off from the gripping pipe member 63, the air cylinder 64 drives the moving member 65 to move down, and the moving member drives the pushing member to move down relative to the gripping pipe member 63 to push the frozen pipe 22 away from the gripping pipe member 63; grab quantity, the position of pipe fitting 63 with it is corresponding to freeze quantity, the position of depositing pipe 22, grab pipe fitting 63 with it is the same to push away the quantity correspondence of piece, works as when freezing pipe 22 has a plurality ofly, can set up a plurality ofly and grab pipe fitting 63 and be used for a plurality of freeze batch transfer of depositing pipe 22, can set up a plurality ofly push away the piece, can realize a plurality ofly freeze batch separation of depositing pipe 22, improve work efficiency.

As a preferred embodiment, the batch grabbing and moving mechanism 60 further includes a guide block fixedly disposed on one side of the grabbing part 62 close to the moving part, and a guide shaft slidably disposed on the guide block, one side of the guide shaft far from the guide block is fixedly connected to the grabbing part 62, it can be understood that the moving part moves up and down to drive the guide shaft, a guide groove disposed obliquely is formed on the guide block, when the guide shaft slides in the guide groove, one side of the grabbing part 62 is hinged to rotate relative to the base shell 61, and the other side moves in a direction close to or far from the moving part, so that the grabbing part 62 opens or closes, and the batch grabbing and moving mechanism 60 clamps or releases the frozen shell 21.

In order to install consumables such as centrifuge tube 110, consumable storage device 150 further includes at least one positioning rack 152 installed on carrier 151, and carrier 151 is the temporary storage point of all kinds of consumables to be taken, and the consumables can be pipette tip 111, centrifuge tube 110, disposable cell count board etc.. In one embodiment, as shown in fig. 8 to 9, the culture operation module 100 further comprises a pipette tip 111 installed on the carrier 151 and used for transferring liquid, and the gripping and transferring device further comprises a pipetting mechanism 70, wherein the pipette tip 111 is installed on the pipetting mechanism 70.

Under the combined action of the pipetting mechanism 70 and the pipetting tip 111, the centrifugal tube 110 is repeatedly blown to make the centrifuged cell liquid become suspension cell liquid, or the adherent cells digested by digestive enzymes in the culture bottle 30 are repeatedly blown to become suspension cell liquid; of course, the cells in the centrifuge tube 110 and the culture flask 30 may be shaken into the cell suspension by the cooperation of the gripping mechanism 50 and the robot arm 41.

In one embodiment, the culture storage mechanism further includes a culture bottle 30 inoculated with cells, the culture operation module 100 further includes a liquid processing device 160 for transferring reagents into the culture bottle 30 or the centrifuge tube 110, as shown in fig. 10, the liquid processing device 160 includes a pipetting line 161, a heating assembly 162 and a pump body 163 both mounted on the pipetting line 161, and a sample adding member 164 communicated with one end of the pipetting line 161, and the other end of the pipetting line 161 is communicated with the culture bottle 30 or the centrifuge tube 110.

The liquid processing apparatus 160 is used to heat the biological reagent stored in the refrigerator 170 or other biological reagents at other positions before entering the centrifuge tube 110 or the culture storage mechanism, the heating unit 162 is used to return the liquid to normal temperature, and the heating unit 162 heats the liquid by means including, but not limited to, water bath heating, oil bath heating, metal bath heating, hot air heating, and the like.

It will be appreciated that the sample application member 164 is a storage carrier for the liquid to be transferred, and may be a bottle of any volume and shape, and the pipette line 161 is used to transfer the liquid from the sample application member 164 to the culture bottle 30 or channels within the centrifuge tube 110, under the action of the pump 163, the liquid in the sample loading member 164 is transferred into the heating assembly 162 through the liquid transfer pipeline 161, heated by the heating assembly 162 and then transferred into the culture bottle 30 or the centrifuge tube 110, and the liquid processing device 160 is provided, when a reagent with proper temperature is needed in the cell culture process, the reagent can be obtained inside, the reagent transfer operation in the cell production process is better ensured, the complex cell production requirement is further met, meanwhile, waiting personnel for allocation from the outside is not needed, great convenience is brought to cell production, and the closed independent operation of the biological tissue production system is better ensured.

In other embodiments, the culture operation module 100 may further include a microscope 171 for observing the cell culture conditions in real time, a centrifuge 172 for separating particles from the suspension, a refrigerator 170 for cryogenically storing the reagents, a clamping and moving warehouse for storing the clamping and moving device, and a device for processing liquid and solid wastes.

Cultivate module 200, including cultivateing box body, sensor module 201, air duct 202 and air duct two 203, cultivate the box body be formed with cultivate cavity 204 and with cultivate the cavity 204 interval set up hold the chamber 205, sensor module 201 set up in hold in the chamber 205, air duct 202 is worn to locate in the lateral wall of cultivateing the box body, the one end of air duct 202 communicate in cultivate cavity 204, the other end communicate in hold chamber 205, the one end of air duct two 203 communicate in hold chamber 205, the other end communicate in cultivate cavity 204.

It is to be understood that; as shown in fig. 11, the gas in the culture cavity 204 can flow into the first accommodating cavity 205 through the first gas guide tube 202, the sensor module 201 detects the gas in the first accommodating cavity 205 to obtain information such as corresponding temperature, humidity and carbon dioxide concentration in real time, and then the gas flowing into the first accommodating cavity 205 flows back to the culture cavity 204 provided with the culture storage mechanism through the second gas guide tube 203.

In one embodiment, the culture module 200 further comprises a rotary culture tray 210, the rotary culture tray 210 comprises a rotary seat 211 partially penetrating into the culture cavity 204, a rotary motor 212, a rotary disk 213 and at least one culture shelf 214, the rotary motor 212 is disposed on the rotary seat 211 and is in driving connection with the rotary disk 213, each culture shelf 214 is detachably mounted on one side of the rotary disk 213 facing away from the rotary seat 211, the rotary motor 212 is located outside the culture box body, the rotary disk 213 is located in the culture cavity 204, it can be understood that, as shown in fig. 12, the rotary culture tray 210 is used for carrying a culture storage mechanism such as a culture bottle 30 to be cultured, and thus, the rotary motor 212 and the rotary seat 211 are matched to provide a rotary driving force for the rotary disk 213 to rotate the rotary disk 213 around its central axis, thereby drive the cultivation frame 214 that is located on rotary disk 213 and rotate, rotating electrical machines 212, partial roating seat 211 are located the outside of cultivateing the box body, and in the in-process of carrying out high temperature sterilization to cultivateing cavity 204 inside, can effectively protect rotating electrical machines 212's normal operating.

In other embodiments, in order to realize the operation of moving in and out the culture storage mechanism inside the housing 300 provided with the culture module 200, as shown in fig. 13, the culture box body is provided with an automatic door 220 for opening and closing, and a manipulator 230 arranged inside the housing 300, wherein the manipulator 230 and the culture box body are arranged at an interval, so that the manipulator 230 moves the culture storage mechanism into the culture cavity 204 or takes out the culture storage mechanism from the culture cavity 204, and the manipulator 230 can also be used for rotating the culture bottle 30 into or out of the culture module 200, thereby facilitating the transfer of the objects among the modules, and the automatic door 220 component plays a role of isolating the culture cavity from the manipulator 230, thereby effectively protecting the manipulator 230 during the high-temperature sterilization process inside the culture cavity 204.

In one embodiment, the biological tissue production system includes an environmental control mechanism 400 and a plurality of housings 300, the functional module group is provided in plurality, each of the culture operation modules 100 or each of the culture modules 200 is installed in each of the housings 300 in a one-to-one correspondence, the environmental control mechanism 400 includes a regulation box 401 installed on the top of the housing 300, a first centrifugal fan 403 installed in the regulation box 401 and installed in the regulation box 401, a first primary filter 402 provided in communication with the outside, a first high efficiency filter 404 provided in communication with the inside of the housing 300, and a first sensor group 405 installed on the regulation box 401. In other embodiments, each of the functional module groups is installed in the housing 300 in a one-to-one correspondence.

It can be understood that, as shown in fig. 14, the working environment inside each housing 300 can be regulated in real time, external air enters the regulation and control box 401 after being primarily filtered by the first primary filter 402, the first centrifugal fan 403 can form an air flow to drive the air to flow when operating, so as to provide a driving force for air circulation, and the air inside the regulation and control box 401 can be driven by the first centrifugal fan 403 to flow inside each housing 300 after being secondarily filtered by the first high efficiency filter 404; the first high efficiency filter 404 can effectively improve the cleanliness of the air entering the casing 300, and on the other hand, the air can form parallel air flow from top to bottom under the action of the first centrifugal fan 403.

In order to facilitate the constant temperature and humidity regulation of the gas in the regulation and control box 401, a first temperature and humidity controller 406 is installed inside the regulation and control box 401, and in order to perform disinfection and sterilization treatment on the internal environment of the shell 300, a first sterilization actuator 407 is installed inside the regulation and control box 401, external air can enter the regulation and control box 401 after being primarily filtered by the first primary filter 402, the first temperature and humidity controller 406 performs constant temperature and humidity treatment on the filtered air, and the first sterilization actuator 407 can rapidly generate ozone gas or hydrogen oxide vapor so as to be mixed with the air after the constant temperature and humidity treatment when the inside of each shell 300 needs to be disinfected and sterilized and flow into each shell 300 after being secondarily filtered by the first high efficiency filter 404.

First sensor group 405 can the inside environmental condition of real-time supervision casing 300, first sensor group 405 at least part stretch into to inside the casing 300, so that real-time assay feedback to temperature and humidity controller department when the inside environmental condition of casing 300 to guarantee temperature and humidity controller to each casing 300 inside environment control's accuracy. Preferably, the first sensor set 405 may be selected from one or more of a wind speed sensor, a humidity sensor, a temperature sensor, an ozone concentration sensor, and a hydrogen peroxide concentration sensor.

In one embodiment, the biological tissue production system further comprises a control module 500 electrically connected to the functional module set, the control module 500 comprises a positioning column 501, an electric control cabinet 502 fixed on the positioning column 502, a torsion shaft 504 rotatably disposed on the positioning column 501, and a control panel 503 fixedly connected to the torsion shaft 504, it can be understood that, as shown in fig. 15, the control module 500 can be fixedly connected to the functional module set through a fixing interface 710 disposed on the positioning column 501, the control module 500 can be electrically connected to the functional module set through the control interface 700, the electric control cabinet 502 is used for controlling the operation of the biological tissue production system, the control panel 503 is a user interface, the control panel 503 has a touch screen 505, an alarm 506, a signal lamp 507, and an emergency stop switch 508, which can refer to the prior art, the torsion shaft 504 allows the control panel 503 to freely rotate with respect to the positioning post 501, and the use angle is adjusted.

The culture operation module 100 and the culture module 200 are controlled by their internal controllers, and the controllers in the culture operation module 100 or the culture module 200 may control other modules, or the operation and control module 500 may collectively manage and operate the modules.

In one embodiment, the biological tissue production system further comprises an external environment controller 600, as shown in fig. 1, the external environment controller 600 comprises an air casing 601, a partition plate 602, a second primary filter 603, a second centrifugal fan 604, a second temperature and humidity controller 605, a second sterilization actuator 606, a second sensor group 607, an air supply pipeline 608, and a second high efficiency filter 609, and the partition plate 602 divides the air casing 601 into an upper air pressure bin and a lower air inlet bin; the second primary filter 603 is vertically installed on the side of the wind shell 601, the second sensor group 607 is located at a position where the pressure cavity leads to the air supply pipeline 608, and is used for measuring the environment inside the equipment and feeding back to the external environment controller 600 in real time to ensure the accuracy of the internal environment control of the biological tissue production system, the air supply pipeline 608 connects the pressure cavity and each module, and delivers the clean high-pressure air flow processed by the external environment controller 600 to each module through the air supply pipeline 608, the second high-efficiency filter 609 is located at the tail end and the top of each module from the air supply pipeline 608, that is, the clean high-pressure air flow delivered to each module by the air supply pipeline 608 is finally filtered by the second high-efficiency filter 609 to form a vertically downward parallel air flow meeting the cleanliness requirement.

It should be noted that the working principle of the second centrifugal fan 604, the second primary filter 603, the second temperature and humidity controller 605 and the second sterilization actuator 606 can refer to the environment control mechanism 400; the external environmental controller 600 is additionally provided for the biological tissue production system, and can be used in cooperation with the culture operation module 100 and the culture module 200, so that all functions of the environmental control system built in each module can be realized.

In summary, in the embodiments of the present invention, at least one culture operation module 100 and at least one culture module 200 are connected to each other through the fixed interface 710 and the control interface 700 according to the culture conditions of the cells, in addition, the external environment controller 600 may be additionally installed according to the actual needs, the environment control mechanisms 400 in the culture operation modules 100 and the culture modules 200 are respectively started, the interiors of the corresponding modules are sterilized, and after the sterilization is completed, the temperature and humidity control is performed on the interiors of the corresponding modules by the environment control mechanisms 400, or the sterilization and temperature and humidity control operations are performed by the external environment controller 600.

When the cell strains to be amplified are cells with the freezing tubes in a frozen state, the freezing tubes 22 are transferred to the thawing and cover opening device 120 through the batch grabbing mechanism 60 or the clamping mechanism 50, the thawing and cover opening device 120 thaws and opens the freezing tubes 22, the clamping mechanism 50 takes out the centrifuge tube 110 from the consumable storage device 150, the cell strains in the freezing tubes can be transferred into the centrifuge tube 110 through the liquid transfer mechanism 70 and the liquid transfer gun heads 111, the centrifuge tube 172 is centrifuged to obtain new culture bottles 30 from the consumable storage device 150, the culture bottles are subpackaged into a plurality of new culture bottles 30 and are filled with reagents according to the density of cell sap, the clamping mechanism 50 transfers the cell strains into the culture module 200 for cell culture, and the adherent cells in the culture bottles 30 are transferred out of the biological tissue production system through the culture operation module 100 after the cell strains are cultured.

When the cell strain to be amplified is adherent cells inoculated to the culture bottle 30, judging whether the cell strain to be inoculated in the culture bottle 30 meets the passage requirement, if the cell strain meets the passage requirement, performing passage operation, digesting the adherent cells by digestive enzymes, transferring the cell strain in the culture bottle 30 into the centrifuge tube 110 by the sucking and spitting action of the liquid transfer mechanism 70 and the liquid transfer gun head 111 or the shaking of the mechanical arm 41 and the clamping mechanism 50, so that the adherent cells in the culture bottle 30 become suspension cell liquid, taking the centrifuge tube 110 out of the consumable storage device 150 by the clamping mechanism 50, transferring the cell strain in the culture bottle 30 into the centrifuge tube 110 through the liquid transfer mechanism 70 and the liquid transfer gun head 111, centrifuging by the centrifuge 172, obtaining a new culture bottle 30 from the consumable storage device 150, subpackaging the new culture bottle 30 into a plurality of culture bottles 30 according to the density of the cell liquid, injecting reagents, transferring the cell strain into the culture module 200 by the clamping mechanism 50 for cell culture, transferring adherent cells in the culture bottle 30 to the outside of the biological tissue production system through the culture operation module 100 after the culture is completed; if the cell strain to be inoculated in the culture bottle 30 does not meet the requirement of passage, the culture bottle 30 is directly sent into the culture module 200 for continuous culture.

The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.

Claims

1. A biological tissue production system, comprising: at least one functional module group, wherein each functional module group comprises at least one culture operation module (100) and at least one culture module (200) which is connected with the culture operation module (100) and is communicated with the culture operation module;

the culture operation module (100) comprises a thawing and uncovering device (120) for thawing and uncovering a culture storage mechanism frozen and stored by the culture storage mechanism used for inoculating cell strains, a consumable storage device (150) used for installing consumables, a clamping and moving device used for clamping the consumables and/or the culture storage mechanism, and a liquid processing device (160) used for processing and moving reagents;

the culture module (200) comprises a culture box body, a rotary culture disc (210) at least partially arranged in the culture box body, an automatic door (220) arranged on the culture box body, and a manipulator (230) arranged at an interval with the culture box body;

wherein, press from both sides the device of moving will centrifuging tube (110) on consumptive material storage device (150) move to the target location, and will freeze and deposit culture storage mechanism shifts to unfreeze uncapping device (120), warp unfreeze uncapping device (120) and recover to the normal atmospheric temperature and open culture storage mechanism will the cell sap among the culture storage mechanism shifts to in centrifuging tube (110) and carry out centrifugal treatment, again through partial shipment inoculation to new culture storage mechanism after, by press from both sides the device of moving and shift to in the culture box body and set up in carry out cell culture on rotatory culture dish (210), the cultivation is accomplished the back warp culture operation module (100) shift to outside the biological tissue production system.

2. The biological tissue production system according to claim 1, wherein the clamping and moving device comprises a clamping mechanism (50) for clamping the centrifuge tube (110) and/or the culture storage mechanism, the clamping mechanism (50) comprises a first driving member (51), and a plurality of clamping groups (52) fixed on the first driving member (51), each clamping group (52) is clamped on the outer surface of the centrifuge tube (110) or the culture storage mechanism, and each clamping group (52) comprises a plurality of clamping members (53) arranged in a surrounding manner along the side surface of the first driving member (51).

3. The biological tissue production system according to claim 1, wherein the clamping and moving device further comprises a batch clamping and moving mechanism (60), the batch clamping and moving mechanism (60) comprises a base shell (61), a clamping member (62) hinged on the base shell (61), and a clamping pipe member (63) installed on the bottom surface of the base shell (61), the culture storage mechanism comprises a freezing assembly, the freezing assembly comprises a freezing shell (21) and a freezing pipe (22) installed in the freezing shell (21), one side of the clamping member (62) far away from the base shell (61) is clamped on the outer surface of the freezing shell (21), and one side of the clamping pipe member (63) far away from the base shell (61) is inserted and fixed in the freezing pipe (22).

4. The biological tissue production system according to claim 3, wherein the batch grabbing and moving mechanism (60) further comprises a cylinder (64) disposed on one side of the base shell (61) far away from the grabbing pipe (63), a moving member fixedly connected to an output end of the cylinder (64) and mounted in the base shell (61), and a pushing member fixed on one side of the moving member far away from the cylinder (64) and penetrating the grabbing pipe (63), wherein the moving member and the grabbing member (62) are slidably disposed relative to each other.

5. The biological tissue production system according to claim 1, wherein the consumable storage device (150) further comprises a carrier (151), and at least one positioning rack (152) mounted on the carrier (151).

6. The biological tissue production system according to claim 5, wherein the gripping device further comprises a pipetting mechanism (70), the cultivation manipulator module (100) further comprises a pipette tip (111) mounted on the carrier (151) for transferring liquid, the pipette tip (111) being mounted on the pipetting mechanism (70).

7. The biological tissue production system according to claim 1, wherein the liquid processing device (160) comprises a pipetting line (161), a heating assembly (162) and a pump body (163) both mounted on the pipetting line (161), and a sample adding member (164) in communication with one end of the pipetting line (161), the culture storage mechanism further comprises a culture bottle (30) inoculated with cells, and the other end of the pipetting line (161) is in communication with the culture bottle (30) or the centrifuge tube (110).

8. The biological tissue production system according to claim 1, wherein the culture module (200) further comprises a sensor assembly (201), a first air duct (202) and a second air duct (203), the culture box body is formed with a culture cavity (204) and a first accommodating cavity (205) spaced from the culture cavity (204), the sensor assembly (201) is arranged in the first accommodating cavity (205), the first air duct (202) is arranged in the side wall of the culture box body in a penetrating manner, one end of the first air duct (202) is communicated with the culture cavity (204), the other end of the first air duct is communicated with the first accommodating cavity (205), one end of the second air duct (203) is communicated with the first accommodating cavity (205), and the other end of the second air duct is communicated with the culture cavity (204); the rotary culture disc (210) comprises a rotary seat (211), a rotary motor (212), a rotary disc (213) and at least one culture frame (214), wherein the part of the rotary seat (211), the rotary motor (212), the rotary disc (213) and the at least one culture frame (214) penetrate into the culture cavity (204), the rotary motor (212) is arranged on the rotary seat (211) and is in driving connection with the rotary disc (213), each culture frame (214) is detachably arranged on one side, back to the rotary seat (211), of the rotary disc (213), the rotary motor (212) is positioned outside the culture box body, and the rotary disc (213) is positioned in the culture cavity (204).

9. The biological tissue production system according to claim 1, further comprising an environmental control mechanism (400) and a plurality of housings (300), wherein the plurality of functional modules are provided, each of the culture operation modules (100) or each of the culture modules (200) is installed in each of the housings (300) in a one-to-one correspondence manner, the environmental control mechanism (400) comprises a control box (401) installed on the top of the housing (300), a first primary filter (402) installed in the control box (401) and arranged to communicate with the outside, a first centrifugal fan (403) installed in the control box (401), a first high efficiency filter (404) arranged to communicate with the inside of the housing (300), and a first sensor group (405) installed on the control box (401); biological tissue production system still includes external environmental controller (600), external environmental controller (600) include wind shell (601), are used for separating division board (602) of wind shell (601), install in second primary filter (603) of wind shell (601) side, set up in second centrifugal fan (604) on division board (602), install in second temperature and humidity controller (605) and second sterilization executor (606) in wind shell (601), with supply air duct (608) that wind shell (601) inside intercommunication set up, install in wind shell (601) and be close to the second sensor group (607) of supply air duct (608) tip to and set up on the surface of each module and with supply air duct (608) connect the second high efficiency filter (609) that sets up.

10. The system according to claim 1, further comprising a control module (500) electrically connected to the functional module set, wherein the control module (500) comprises a positioning column (501), an electric control cabinet (502) fixed to the positioning column, a torsion shaft (504) rotatably disposed on the positioning column (501), and a control panel (503) fixed to the torsion shaft (504).