CN113755331A

CN113755331A - Cell culture case of simulation plateau environment

Info

Publication number: CN113755331A
Application number: CN202111215327.7A
Authority: CN
Inventors: 孙红玉; 黄启林
Original assignee: Western Theater General Hospital of PLA
Current assignee: Western Theater General Hospital of PLA
Priority date: 2021-10-19
Filing date: 2021-10-19
Publication date: 2021-12-07
Anticipated expiration: 2041-10-19
Also published as: CN113755331B

Abstract

The invention belongs to the field of biological cell culture, and particularly relates to a cell culture box for simulating a plateau environment, which comprises a box body assembly, wherein a culture area, an observation area and a supply area are arranged on the inner side of the box body assembly, the culture area and the observation area are positioned above the supply area, a control panel is arranged on the upper part of one outer side of the box body assembly, the observation area is positioned on one side, close to the control panel, in the box body assembly, and a protective door is arranged below the control panel. The invention can simulate the cell culture process in the plateau environment.

Description

Cell culture case of simulation plateau environment

Technical Field

The invention belongs to the field of biological cell culture, and particularly relates to a cell culture box for simulating a plateau environment.

Background

The cell culture box for simulating the plateau environment by controlling the oxygen partial pressure and the temperature and humidity is scientific research equipment for simulating the plateau environment, and can be applied to relevant life science research and biological research of plateaus. The existing cell culture box can only control and adjust the air temperature and humidity and the oxygen and carbon dioxide content, but lacks the air pressure condition and the magnetic field environment in the plateau, so that the growth state of cells in the plateau environment cannot be expressed really; when observing the cell solution in the culture device, the extracted sample is generally required to be transferred out of the culture device and then observed, and the sterile environment of the sample is easily damaged and easily polluted in the process, so that the observation result is influenced, and the workload is large; at present, a three-gas incubator is adopted to control oxygen concentration by flushing nitrogen to simulate a plateau anoxic environment, but the real plateau environment is that the oxygen content is the same as the plain content and is 21 percent, and only the plateau atmospheric pressure is reduced, so that the oxygen supply is insufficient. The pure control of the oxygen concentration to simulate the plateau environment ignores low-pressure factors and temperature and humidity factors, and cannot truly reflect the influence of the plateau environment on organisms in the research of in vitro cell level. Therefore, a cell culture box simulating a plateau environment is needed.

Disclosure of Invention

The invention aims to provide a cell incubator for simulating a plateau environment, which solves the problems.

In order to achieve the purpose, the invention provides the following scheme: a cell culture box for simulating a plateau environment comprises a box body assembly, wherein a culture area, an observation area and a supply area are arranged on the inner side of the box body assembly, the culture area and the observation area are positioned above the supply area, a control panel is arranged on the upper part of one outer side of the box body assembly, the observation area is positioned on one side, close to the control panel, in the box body assembly, and a protective door is arranged below the control panel;

a magnetic field generator and a vacuum pump are arranged in the supply area, the magnetic field generator is correspondingly arranged below the culture area, the vacuum pump is communicated with the culture area, a carbon dioxide air inlet, an air inlet and an electromagnetic pressure valve are fixedly connected to one side of the box body assembly, which is far away from the protective door, and the carbon dioxide air inlet, the air inlet and the electromagnetic pressure valve are respectively communicated with the culture area; the observation area is used for observing the cell culture state, and the supply area is also used for supplying culture solution;

a PLC controller is arranged in the control panel and is electrically connected with the electromagnetic pressure valve, the magnetic field generator and the vacuum pump; and a pressure sensor, a temperature and humidity sensor, a carbon dioxide concentration sensor and an oxygen concentration sensor which are electrically connected with the PLC are arranged in the culture area.

Preferably, the culture area sequentially comprises an original culture chamber, a magnetic control culture chamber, a slide storage chamber and an instrument storage chamber, the slide storage chamber is positioned above the instrument storage chamber, culture bottles are respectively placed in the original culture chamber and the magnetic control culture chamber, a micro pump and a dropper are sequentially connected to the outer parts of one sides of the original culture chamber and the magnetic control culture chamber, which are close to the control panel, and the inlet end of the micro pump is communicated with the culture bottles;

the magnetic field generator is correspondingly arranged below the magnetic control culture chamber, the carbon dioxide air inlet, the air inlet and the electromagnetic pressure valve are respectively communicated with the original culture chamber and the magnetic control culture chamber, and the micropump is electrically connected with the PLC.

Preferably, a sealing plug is inserted into a bottle opening of the culture bottle, a liquid discharge pipe, a liquid inlet pipe, a circulating air inlet channel and a circulating air outlet channel penetrate through the sealing plug from top to bottom, and a stirring mechanism is arranged below the sealing plug; the outlet end of the liquid discharge pipe is communicated with the inlet end of the micro pump, the liquid inlet pipe is communicated with the supply area, the circulating air inlet channel is communicated with the carbon dioxide air inlet and the air inlet, and the outlet end of the circulating air outlet channel is communicated with the vacuum pump.

Preferably, the stirring mechanism comprises a micro motor fixedly connected to the lower end of the sealing plug, one end of a rotating shaft is connected to the lower portion of the micro motor in a shaft coupling mode, the other end of the rotating shaft is connected with a blade in a shaft coupling mode, one end of a fixing rod is fixedly connected to the lower portion of the micro motor in a shaft coupling mode, the rotating shaft is rotatably connected to an inner cavity of the fixing rod, the other end of the fixing rod is rotatably connected with the blade, and the micro motor is electrically connected with the PLC.

Preferably, be provided with a plurality of layers of baffle in the slide storeroom, a plurality of recesses have been seted up on the baffle, keep away from in the slide storeroom a plurality of spacing archs of fixedly connected with on control panel's the lateral wall, spacing protruding with the recess corresponds the setting, the bottom of slide has been placed to the slope in the recess, the middle part butt of slide is in on the spacing arch, a plurality of intercommunicating pores have been seted up to the bottom of recess, the bottommost the baffle below is provided with the slide and deposits the district, keep away from outside the slide storeroom be provided with the transmission door on control panel's the lateral wall and communicate and have the second vacuum hole, the transmission door with the slide is deposited the district and is corresponded the setting.

Preferably, the top of the inner side of the instrument storage chamber is connected with an ultraviolet lamp, two opposite side walls in the instrument storage chamber are respectively connected with a hook and a disinfection spray head, the hook is positioned below the disinfection spray head, and the bottom of the instrument storage chamber is provided with a first vacuum hole.

Preferably, a microscope is arranged above the inner side of the observation area, an observation port of the microscope is located at the top of the outer side of the box body assembly, an observation layer is arranged between the microscope and the protective door, two operation holes are formed in the observation layer, and operation gloves are fixedly connected to the edges of the operation holes.

Preferably, a liquid inlet pump and a liquid outlet pump are further arranged at the top of the inner side of the supply area, the inlet end of the liquid inlet pump is communicated with a culture solution bottle through a first liquid passing pipe, the outlet end of the liquid outlet pump is communicated with a metabolite bottle through a second liquid passing pipe, the outlet end of the liquid inlet pump is communicated with the inlet end of the liquid inlet pipe, the inlet end of the liquid outlet pump is communicated with the outlet end of the liquid outlet pipe, and the side wall, far away from the control panel, in the supply area is communicated with an exhaust port.

Preferably, an isolation layer is arranged on the periphery of the magnetic control culture chamber, and the isolation layer is made of superconducting materials and used for limiting the action range of a magnetic field of the magnetic field generator.

Preferably, one side of the observation layer, which is close to the protective door, is provided with a tempered glass explosion-proof film.

The invention has the following technical effects: the main function of the culture area is to culture cells; the observation area is mainly used for observing the growth state of the cells in the culture process; the magnetic field generator is used for providing a magnetic field of a plateau area for partial cells in the culture area, so that a contrast test with other cells is facilitated; the vacuum pump is used for providing a high-altitude air pressure environment for cell culture, and the oxygen partial pressure can be controlled through the vacuum pump; the carbon dioxide air inlet and the air inlet provide clean mixed air close to plateau areas for cell culture; the observation area is used for observing the cell culture state, and the supply area is also used for supplementing culture solution for cultured cells; the regulation can be released through an electromagnetic pressure valve; through the control panel, can carry out initiative adjustment according to the environmental change of reacting in the cell culture process.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is an overall schematic view of the present invention;

FIG. 2 is another schematic view of the present invention;

FIG. 3 is a front cross-sectional view of the present invention;

FIG. 4 is a schematic illustration of the internal distribution of the present invention;

FIG. 5 is a schematic view of the interior of the slide storage chamber;

wherein, 1, a box body assembly; 2. a control panel; 3. an upper outer door; 4. a lower outer door; 5. a microscope; 6. a carbon dioxide gas inlet; 7. an air inlet; 8. an electromagnetic pressure valve; 9. an exhaust port; 10. an isolation layer; 11. a liquid discharge pipe; 12. a liquid inlet pipe; 13. a sealing plug; 14. a micro motor; 15. fixing the rod; 16. a rotating shaft; 17. a blade; 18. a liquid inlet pump; 19. a liquid discharge pump; 20. a first liquid through pipe; 21. a second liquid through pipe; 22. a culture solution bottle; 23. a metabolite bottle; 24. a magnetic field generator; 25. a first vacuum hole; 26. hooking; 27. a disinfection nozzle; 28. an ultraviolet lamp; 29. a second vacuum hole; 30. a pass-through gate; 31. glass slide; 32. an original culture chamber; 33. a magnetic control culture chamber; 34. a slide storage chamber; 35. an instrument storage compartment; 36. a limiting bulge; 37. a partition plate; 38. a communicating hole; 39. an observation layer; 40. an operation hole; 41. a micro-pump; 42. a dropper; 43. circulating an air inlet pipe; 44. circulating the air outlet pipe; 45. a culture bottle; 46. a vacuum pump.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

Referring to fig. 1-5, the invention provides a cell incubator for simulating a plateau environment, comprising a case assembly 1, wherein a culture area, an observation area and a supply area are arranged on the inner side of the case assembly 1, the culture area and the observation area are positioned above the supply area, a control panel 2 is arranged on the upper part of one outer side of the case assembly 1, the observation area is positioned on one side, close to the control panel 2, in the case assembly 1, and a protective door is arranged below the control panel 2;

a magnetic field generator 24 and a vacuum pump 46 are arranged in the supply area, the magnetic field generator 24 is correspondingly arranged below the culture area, a carbon dioxide air inlet 6 and an air inlet 7 are arranged on one side of the box body assembly 1 away from the protective door, and an electromagnetic pressure valve 8 is fixedly connected with the side, and the carbon dioxide air inlet 6, the air inlet 7 and the electromagnetic pressure valve 8 are respectively communicated with the culture area; the observation area is used for observing the cell culture state, and the supply area is also used for supplying culture solution;

a PLC controller is arranged in the control panel 2 and is electrically connected with the electromagnetic pressure valve 8, the magnetic field generator 24 and the vacuum pump 46; and a pressure sensor, a temperature and humidity sensor, a carbon dioxide concentration sensor and an oxygen concentration sensor which are electrically connected with the PLC are arranged in the culture area. The main function of the culture area is to culture cells; the observation area is mainly used for observing the growth state of the cells in the culture process; the magnetic field generator 24 is used for providing a magnetic field of a plateau area for partial cells in the culture area, so that comparison tests with other cells are facilitated; the vacuum pump 46 is used for providing a high atmospheric pressure environment for cell culture, and the oxygen partial pressure can be controlled through the vacuum pump 46; the carbon dioxide air inlet 6 and the air inlet 7 provide clean mixed air close to plateau areas for cell culture; the observation area is used for observing the cell culture state, and the supply area is also used for supplementing culture solution for cultured cells; the regulation can be released by means of the electromagnetic pressure valve 8; the active adjustment can be performed according to the environmental change of the reaction during the cell culture process by the control panel 2.

According to a further optimized scheme, the culture area sequentially comprises an original culture chamber 32, a magnetic control culture chamber 33, a slide storage chamber 34 and an instrument storage chamber 35, the slide storage chamber 34 is located above the instrument storage chamber 35, culture bottles 45 are respectively placed in the original culture chamber 32 and the magnetic control culture chamber 33, a micro pump 41 and a dropper 42 are sequentially connected to the outer portions of one sides, close to the control panel 2, of the original culture chamber 32 and the magnetic control culture chamber 33, and the inlet end of the micro pump 41 is communicated with the culture bottles 45;

the magnetic field generator 24 is correspondingly arranged below the magnetic control culture chamber 33, the carbon dioxide air inlet 6, the air inlet 7 and the electromagnetic pressure valve 8 are respectively communicated with the original culture chamber 32 and the magnetic control culture chamber 33, and the micro pump 41 is electrically connected with the PLC. The cells cultured in the original culture chamber 32 grow in the current magnetic field environment, the cells cultured in the magnetic control culture chamber 33 grow in the plateau simulation magnetic field environment, the micro pump 41 and the dropper 42 are used for pumping the cell solution in the culture bottle 45 when needed, the growth state of the cells is observed and recorded through the observation area, the culture environments introduced into the original culture chamber 32 and the magnetic control culture chamber 33 are the same, and the pressure in the original culture chamber 32 and the pressure in the magnetic control culture chamber 33 are adjusted through the electromagnetic pressure valve 8.

According to a further optimized scheme, a sealing plug 13 is inserted into the mouth of the culture bottle 45, a liquid discharge pipe 11, a liquid inlet pipe 12, a circulating air inlet channel 43 and a circulating air outlet channel 44 penetrate through the sealing plug 13 from top to bottom, and a stirring mechanism is arranged below the sealing plug 13; the outlet end of the liquid discharge pipe 11 is communicated with the inlet end of the micro pump 41, the liquid inlet pipe 12 is communicated with the supply area, the circulating gas inlet channel 43 is communicated with the carbon dioxide gas inlet 6 and the air gas inlet 7, and the outlet end of the circulating gas outlet channel 44 is communicated with the vacuum pump 46. Fresh culture solution is supplemented into the culture bottle 45 through the liquid inlet pipe 12, used culture solution is replaced for the culture bottle 45 through the liquid outlet pipe 11, meanwhile, a cell culture solution observation sample can be obtained through the liquid outlet pipe 11, the circulating air inlet channel 43 and the circulating air outlet channel 44 are used for ensuring air flow and air quality in the culture bottle 45, and the circulating air outlet channel 44 is used for extracting metabolic air under the action of the vacuum pump 46; the stirring mechanism is mainly used for floating growing cells, and the cells move to the edge under the action of centrifugal force and then are pumped out of a used culture solution in the central position.

Further optimize the scheme, rabbling mechanism includes that fixed connection is at the micro motor 14 of sealing plug 13 lower extreme, and the below of micro motor 14 has the one end of axis of rotation 16, and the other end hub connection of axis of rotation 16 has blade 17, and the below fixedly connected with dead lever 15's of micro motor 14 one end, axis of rotation 16 rotate to be connected in the inner chamber of dead lever 15, and the other end and the blade 17 of dead lever 15 rotate to be connected, micro motor 14 and PLC controller electric connection.

Further optimization scheme, be provided with a plurality of layers of baffle 37 in the slide storeroom 34, a plurality of recesses have been seted up on the baffle 37, keep away from a plurality of spacing archs 36 of fixedly connected with on the lateral wall of control panel 2 in the slide storeroom 34, spacing arch 36 corresponds the setting with the recess, the bottom of slide 31 has been placed to the slope in the recess, the middle part butt of slide 31 is on spacing arch 36, a plurality of intercommunicating pores 38 have been seted up to the bottom of recess, the baffle 37 below of bottommost is provided with the slide and deposits the district, be provided with transmission door 30 and second vacuum hole 29 on the lateral wall of control panel 2 is kept away from outward to slide storeroom 34, transmission door 30 and slide deposit the district and correspond the setting. The slide 31 is used for bearing cell solutions in different stages in cell culture, the growth state of cells is recorded by observing the slide 31, and a plurality of slides 31 are stored in the slide storage chamber 34, so that the cell culture with a long period is facilitated; the used slides 31 are placed at the lowermost end of the slide storage compartment 34, the used slides 31 are then collectively cleaned out through the transfer door 30, and the interior of the slide storage compartment 34 is evacuated through the second vacuum port 29 to ensure a relatively sterile environment.

According to a further optimized scheme, the top of the inner side of the instrument storage chamber 35 is connected with an ultraviolet lamp 28, two opposite side walls in the instrument storage chamber 35 are respectively connected with a hook 26 and a disinfection spray nozzle 27, the hook 26 is located below the disinfection spray nozzle 27, and the bottom of the instrument storage chamber 35 is provided with a first vacuum hole 25. The hook 26 is used for hanging simple and convenient medical instruments, the medical instruments are used for taking the glass slide 31, then the medical instruments are disinfected through the disinfection spray head 27 and the ultraviolet lamp 28, and vacuumizing treatment is carried out through the first vacuum hole 25 after disinfection is finished, so that a relatively sterile environment is guaranteed.

Further optimize the scheme, the inboard top in observation zone is provided with microscope 5, and the viewing aperture of microscope 5 is located the outside top of box assembly 1, is provided with observation layer 39 between microscope 5 and the guard gate, has seted up two handle holes 40 on the observation layer 39, and not shown in the handle glove picture of handle hole 40 edge fixedly connected with. The microscope 5 is combined with the box body assembly 1, so that after a cell growth solution sample is conveniently extracted, the record can be observed in time, and the observation result is prevented from being influenced by the fact that the slide 31 is in contact with the external environment; meanwhile, the microscope 5 is favorable for storage in a relatively sterile environment, and the service life is prolonged.

Further optimizing the scheme, the inside top in supply district still is provided with feed liquor pump 18 and flowing back pump 19, and the entrance point of feed liquor pump 18 communicates through first liquid pipe 20 has the culture solution bottle 22, and the exit end of flowing back pump 19 communicates through second liquid pipe 21 has metabolite bottle 23, and the exit end of feed liquor pump 18 communicates with the entrance point of feed liquor pipe 12, and the entrance point of flowing back pump 19 communicates with the exit end of flowing back pipe 11, and the lateral wall of keeping away from control panel 2 in the supply district communicates with gas vent 9. After the cells grow for a certain time, fresh culture solution needs to be supplemented, and the culture solution in the culture bottle 45 is supplemented and extracted through the liquid inlet pump 18 and the liquid outlet pump 19, so that the purpose of updating is achieved, and the growth of the cells is facilitated.

In a further optimized scheme, the periphery of the magnetic control culture chamber 33 is provided with an isolation layer 10, and the isolation layer 10 is made of superconducting materials and used for limiting the action range of the magnetic field generator 24.

According to a further optimized scheme, one side of the observation layer 39, which is close to the protective door, is provided with a tempered glass explosion-proof film.

Further optimize the scheme, pressure sensor, temperature and humidity sensor, carbon dioxide concentration sensor and oxygen concentration sensor all connect inside blake bottle 45. Environmental change signals in the cell culture process are obtained through the pressure sensor, the temperature and humidity sensor, the carbon dioxide concentration sensor and the oxygen concentration sensor, and are transmitted to the PLC controller, and the PLC controller transmits the converted signals to the electromagnetic pressure valve 8, the micro motor 14, the liquid inlet pump 18, the liquid outlet pump 19, the magnetic field generator 24, the micro pump 41 and the vacuum pump 46, and the signals are correspondingly started according to different changes.

In a further optimized scheme, the original culture chamber 32, the magnetic control culture chamber 33, the slide storage chamber 34 and the instrument storage chamber 35 are all relatively independent spaces, and are respectively provided with a sealing door, and high-temperature-resistant and high-tightness rubber rings are arranged among the sealing doors, the original culture chamber 32, the magnetic control culture chamber 33, the slide storage chamber 34 and the instrument storage chamber 35.

Further optimization scheme, air inlet 7 still communicates there is HEPA high efficiency filtration system. Clean air is provided for the cell culture process.

In a further optimized scheme, the top of the box body assembly 1 is made of a transparent material. The culture bottle 45 is conveniently illuminated, and the cell growth condition is met by adjusting the illumination intensity.

According to the further optimization scheme, the protective door comprises an upper outer layer door 3 and a lower outer layer door 4, the upper outer layer door 3 is arranged corresponding to the observation area, and the lower outer layer door 4 is arranged corresponding to the supply area.

The working process of the embodiment is as follows: in the initial state, cells are cultured in the culture flask 45; after a period of time, the cells in the culture bottle 45 need to be observed, the protective door needs to be opened firstly, then the cell enters the operation hole 40 by one hand, and the operation gloves are worn; then opening the sealing doors of the slide storeroom 34 and the instrument storeroom 35, taking the slide 31 through the medical instrument, operating the control panel 2 with the other hand, sending a starting instruction to the micro pump 41 through the PLC, dripping a proper amount of cell culture solution from the dropper 42 to the slide 31 through the liquid discharge pipe 11, the micro pump 41 and the dropper 42 in sequence, and observing the cell growth state through the microscope 5; after the observation is finished, putting the slide 31 into a slide storage area, keeping the closed state of the sealing door after accumulating the slides 31 in a certain number of observation periods, opening the transfer door 30, and uniformly cleaning; meanwhile, the used medical apparatus is placed in the apparatus storage chamber 35 for disinfection treatment, so that the medical apparatus can be used for the next time. When the air for cell culture needs to be renewed, fresh mixed air is firstly supplemented into the culture bottle 45 through the carbon dioxide air inlet 6, the air inlet 7 and the circulating air inlet pipe 43 in sequence, and then the old air in the culture bottle 45 is pumped out through the circulating air outlet pipe 44 and the vacuum pump 46 in sequence.

The whole device is carried out at room temperature.

In the description of the present invention, it is to be understood that the terms "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, are merely for convenience of description of the present invention, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention.

The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims

1. The utility model provides a cell culture case of simulation plateau environment which characterized in that: the box body assembly comprises a box body assembly (1), wherein a culture area, an observation area and a supply area are arranged on the inner side of the box body assembly (1), the culture area and the observation area are positioned above the supply area, a control panel (2) is arranged on the upper part of one outer side of the box body assembly (1), the observation area is positioned on one side, close to the control panel (2), in the box body assembly (1), and a protective door is arranged below the control panel (2);

a magnetic field generator (24) and a vacuum pump (46) are arranged in the supply area, the magnetic field generator (24) is correspondingly arranged below the culture area, the vacuum pump (46) is communicated with the culture area, a carbon dioxide air inlet (6), an air inlet (7) and an electromagnetic pressure valve (8) are fixedly connected to one side, away from the protective door, of the box body assembly (1), and the carbon dioxide air inlet (6), the air inlet (7) and the electromagnetic pressure valve (8) are respectively communicated with the culture area; the observation area is used for observing the cell culture state, and the supply area is also used for supplying culture solution;

a PLC (programmable logic controller) is arranged in the control panel (2), and the PLC is electrically connected with the electromagnetic pressure valve (8), the magnetic field generator (24) and the vacuum pump (46); and a pressure sensor, a temperature and humidity sensor, a carbon dioxide concentration sensor and an oxygen concentration sensor which are electrically connected with the PLC are arranged in the culture area.

2. The plateau environment-simulating cell incubator of claim 1, wherein: the culture area sequentially comprises an original culture chamber (32), a magnetic control culture chamber (33), a slide storage chamber (34) and an instrument storage chamber (35), the slide storage chamber (34) is positioned above the instrument storage chamber (35), culture bottles (45) are respectively placed in the original culture chamber (32) and the magnetic control culture chamber (33), a micro pump (41) and a dropper (42) are sequentially connected to the outer parts of one sides, close to the control panel (2), of the original culture chamber (32) and the magnetic control culture chamber (33), and the inlet end of the micro pump (41) is communicated with the culture bottles (45);

the magnetic field generator (24) is correspondingly arranged below the magnetic control culture chamber (33), the carbon dioxide air inlet (6), the air inlet (7) and the electromagnetic pressure valve (8) are respectively communicated with the original culture chamber (32) and the magnetic control culture chamber (33), and the micropump (41) is electrically connected with the PLC.

3. A cell incubator according to claim 2, characterised in that: a sealing plug (13) is inserted into the mouth of the culture bottle (45), a liquid discharge pipe (11), a liquid inlet pipe (12), a circulating air inlet channel (43) and a circulating air outlet channel (44) penetrate through the sealing plug (13) from top to bottom, and a stirring mechanism is arranged below the sealing plug (13); the outlet end of the liquid discharge pipe (11) is communicated with the inlet end of the micro pump (41), the liquid inlet pipe (12) is communicated with the supply area, the circulating air inlet channel (43) is communicated with the carbon dioxide air inlet (6) and the air inlet (7), and the outlet end of the circulating air outlet channel (44) is communicated with the vacuum pump (46).

4. The plateau environment-simulating cell incubator of claim 3, wherein: the stirring mechanism comprises a micro motor (14) fixedly connected to the lower end of the sealing plug (13), one end of a rotating shaft (16) is connected to the lower portion of the micro motor (14) in a shaft connection mode, a blade (17) is connected to the other end of the rotating shaft (16) in a shaft connection mode, one end of a fixing rod (15) is fixedly connected to the lower portion of the micro motor (14), the rotating shaft (16) is rotatably connected to the inner cavity of the fixing rod (15), the other end of the fixing rod (15) is rotatably connected with the blade (17), and the micro motor (14) is electrically connected with the PLC.

5. The plateau environment-simulating cell incubator of claim 3, wherein: be provided with a plurality of layers of baffle (37) in slide storeroom (34), a plurality of recesses have been seted up on baffle (37), keep away from in slide storeroom (34) on the lateral wall of control panel (2) a plurality of spacing archs (36) of fixedly connected with, spacing arch (36) with the recess corresponds the setting, the bottom of slide (31) has been placed to the slope in the recess, the middle part butt of slide (31) is in on spacing arch (36), a plurality of intercommunicating pores (38) have been seted up to the bottom of recess, the bottommost baffle (37) below is provided with slide storage area, slide storeroom (34) are kept away from outward be provided with transfer door (30) and second vacuum hole (29) on the lateral wall of control panel (2), transfer door (30) with slide storage area corresponds the setting.

6. The plateau environment-simulating cell incubator of claim 3, wherein: the ultraviolet lamp (28) is connected with the top of the inner side of the instrument storage chamber (35), a hook (26) and a disinfection spray head (27) are respectively connected onto two opposite side walls in the instrument storage chamber (35), the hook (26) is located below the disinfection spray head (27), and a first vacuum hole (25) is formed in the bottom of the instrument storage chamber (35).

7. The plateau environment-simulating cell incubator of claim 1, wherein: the utility model discloses a box assembly, including observation zone, be provided with microscope (5) inboard top, the viewing aperture of microscope (5) is located the outside top of box assembly (1), microscope (5) with be provided with between the guard gate and observe layer (39), two handle holes (40) have been seted up on observing layer (39), handle hole (40) edge fixedly connected with operation gloves.

8. The plateau environment-simulating cell incubator of claim 3, wherein: the top of the inner side of the supply area is further provided with a liquid inlet pump (18) and a liquid discharge pump (19), the inlet end of the liquid inlet pump (18) is communicated with a culture solution bottle (22) through a first liquid passing pipe (20), the outlet end of the liquid discharge pump (19) is communicated with a metabolite bottle (23) through a second liquid passing pipe (21), the outlet end of the liquid inlet pump (18) is communicated with the inlet end of the liquid inlet pipe (12), the inlet end of the liquid discharge pump (19) is communicated with the outlet end of the liquid discharge pipe (11), and the side wall of the control panel (2) which is far away from the supply area is communicated with an exhaust port (9).

9. A cell incubator according to claim 2, characterised in that: an isolation layer (10) is arranged on the periphery of the magnetic control culture chamber (33), and the isolation layer (10) is made of superconducting materials and used for limiting the magnetic field action range of the magnetic field generator (24).

10. The plateau environment-simulating cell incubator of claim 7, wherein: one side of the observation layer (39) close to the protective door is provided with a toughened glass pasted explosion-proof film.