CN110484437A - A kind of high-effective microorganism system for pretreating sample - Google Patents

A kind of high-effective microorganism system for pretreating sample Download PDF

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Publication number
CN110484437A
CN110484437A CN201910757355.8A CN201910757355A CN110484437A CN 110484437 A CN110484437 A CN 110484437A CN 201910757355 A CN201910757355 A CN 201910757355A CN 110484437 A CN110484437 A CN 110484437A
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China
Prior art keywords
culture dish
sample
storehouse
untreated
culture
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CN201910757355.8A
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Chinese (zh)
Inventor
聂晖
王培�
宁春华
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JINAN BAIBO BIOTECHNOLOGY Co Ltd
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JINAN BAIBO BIOTECHNOLOGY Co Ltd
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Priority to CN201910757355.8A priority Critical patent/CN110484437A/en
Publication of CN110484437A publication Critical patent/CN110484437A/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/50Means for positioning or orientating the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M45/00Means for pre-treatment of biological substances
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M99/00Subject matter not otherwise provided for in other groups of this subclass

Abstract

The present invention relates to a kind of high-effective microorganism system for pretreating sample comprising sample bit, sample tube clamping and capping device of uncapping, disinfector, plane motion classification inoculation apparatus, untreated plate position in storehouse, plate push and buckle closure device of uncapping, vaccination station, automatic labeling device, processed plate position in storehouse with shaking table.The present invention replaces traditional-handwork method sample process and streak inoculation using mechanical arm, automatically screw capping carried out to micro-biological samples, barcode scanning, patch code, carry, uncap, upper cover, the sequence of operations such as streak inoculation, the bio-safety that can greatly ensure microorganism staff, improves work efficiency simultaneously.

Description

A kind of high-effective microorganism system for pretreating sample
Technical field
The invention belongs to the field of medical instrument technology, in particular to a kind of high-effective microorganism system for pretreating sample.
Background technique
Clinical microbiology laboratory technique be the fields such as medical institutions' medical diagnosis on disease, antibiotic reasonable employment, hospital infection control not The helper that can or lack.Microorganism sample pre-treatment is the important link in examining, and heavy workload spends the time long, processing result Also the accuracy of subsequent all detections is directly affected.Just because of this, how efficient high texture handles microorganism sample, becomes raising The problem of Micro biological Tests speed, efficiency and quality.
The culture medium streak inoculation work of Microbiological Lab, medical institutions, the country relies primarily on manual operations at present, works For personnel due to the close contact with clinical samples in seeded process, itself also faces the risk of biological infection.In addition, by hand Operation is influenced very big by human factor, and random, the nonstandard parasexuality of operation and error etc. frequently result in sample streak inoculation not When and cause the mistakes of cultivation results.Therefore, a kind of full automatic micro-biological samples processing system with standard processing capacity Seem to market and especially needs.
Summary of the invention
The present invention is in view of the above-mentioned problems, spy proposes a kind of high-effective microorganism system for pretreating sample.
Technical scheme is as follows:
A kind of high-effective microorganism system for pretreating sample, comprising: the sample bit, sample tube clamping with shaking table and upper cover dress of uncapping It sets, disinfector, plane motion classification inoculation apparatus, untreated culture dish storehouse, plate pushes and buckle closure device of uncapping, inoculation Platform, automatic labeling device, processed culture dish storehouse.
Wherein, sample bit includes the shaking table in base plate bottom installation, has a certain number of pores and each on the shaking table The pore size and sample tube of pore match for placing sample tube.
Sample to be examined pipe is placed in the pore of shaking table, after instrument starting, the sample in sample tube shakes with shaking table, The homogenize process that can promote sample improves the recall rate of sample.
Sample tube clamping and capping device of uncapping include the mechanical arm positioned at sample bit side, are equipped with folder on mechanical arm Head can clamp sample tube, be uncapped and upper cover;Above-mentioned sample tube clamping and capping device of uncapping further include being located to take Sample position and the sample pipe clip for being used for fixed sample pipe.
After instrument starting, mechanical arm and collet thereon realize the clamping and transhipment of sample tube, i.e., are grabbed by collet Sample tube (position of collet crawl is the lid on sample tube top) on sample bit, and pass through mechanical arm for sample tube by sample Bit transition is clamped fixation to sample tube to sampling position, then by the sample pipe clip on sampling position;Later, on mechanical arm Collet rotates counterclockwise to be unscrewed sample tube lid and is moved to side;After to be sampled, mechanical arm control collet moves again It above to sample tube, moves downward, when lid is pressed against the nozzle of sample tube, rotating electric machine drives collet rotation by lid It screws, completes upper cover;The collet of mechanical arm control later is moved upwards to be detached from sample tube lid, then sample pipe clip pine It opens, sample tube is fallen into sample tube temporary storage box.
As a kind of preferred structure of collet in above scheme, above-mentioned collet is to be mounted on mechanical arm by rotating electric machine On, the tube wall inner periphery of collet is disposed with spring clip (having spring behind intermediate plate), the bottom end of spring clip be provided with chamfering with The importing of convenience sample pipe lid, the inner surface of spring clip have vertical card line with the vertical line clamping with sample tube exterior surface.Folder When taking: mechanical arm control collet is moved to above sample tube and moves down, and can pack into the lid of sample tube by pressure In the spring clip of collet, the clamping to sample tube is realized;When uncapping: being rotated, can be incited somebody to action counterclockwise by controlling rotating electric machine The unscrewing of sample tube lid;When upper cover, lid is pressed into the nozzle of sample tube by mechanical arm control collet, and rotating electric machine rotates clockwise Lid is screwed and completes upper cover, the collet of mechanical arm control later moves up, and makes spring clip and sample tube using upward pulling force Lid is detached from.
1 to 6 kind of different color can be set into sample tube lid in above scheme, indicates different types of sample, can Different colors, and the culture dish of corresponding different untreated culture dish storage positions are screened by pipe upper part of the cover camera.
It is equipped with scanning machine on the outside of above-mentioned sample tube clamping and capping device of uncapping, with barcode scanning personal information and can be transferred to certainly At dynamic device for labeling.
Disinfector is high-temperature electromagnetic sterilizer.
Plane motion classification inoculation apparatus includes the horizontal rotation support base motor-driven being connected with substrate, is rotated horizontally Support base upper end is equipped with vertical transport (vertical electric pole), is equipped with horizontal transport mechanism in vertical transport, horizontal Oese fixed device is installed in transport mechanism, sets rotating electric machine in the fixed device of oese, drives backspin in rotating electric machine The whole inoculation ring position of modulation.
When work: rotating horizontally support base and rotate oese to high-temperature electromagnetic sterilizer, vertical transport is hung down Oese is directly inserted into high-temperature electromagnetic sterilizer the sterilization that carries out disinfection, after sterilization to be sterilized, vertical transport is vertical Move upwards extraction oese.By rotating horizontally the rotation of support base, oese is rotated to sample tube of having uncapped, downwards Movement dips sample.Support base is rotated horizontally after the completion of sampling to rotate oese to vaccination station.
The bottom of untreated culture dish position in storehouse is connected with rotating electric machine, and circle distribution has a certain number of culture dishes thereon Storage position, the bottom of culture dish storage position have a circle card edge with support culture dish;Side close to vaccination station is provided with position In the culture dish jacking apparatus of culture dish storage position bottom surface and the culture dish push dress being mounted below untreated culture dish position in storehouse It sets, which is made of vertical transport (vertical electric pole) and a culture dish supporting plate being mounted above, Culture dish jacking apparatus is mounted on culture dish driving means;Culture dish lockable mechanism is mounted on untreated culture dish storage position On, the thickness of a culture dish is more than or equal at a distance from culture dish card edge.
When work: culture dish is upside down in culture dish storage position, rotating electric machine of the untreated culture dish position in storehouse in its bottom The lower rotation of effect, the culture dish storage position that will be inoculated with are rotated to the side close to vaccination station.Culture dish is jacked in culture dish and is filled Certain position is vertically moved upward under the effect of setting, plate lockable mechanism locks the plate of bottom second, cultivates immediately Ware jacking apparatus starts slowly to decline playback, and first plate in bottom is released culture dish and store in a warehouse position to recessed by culture dish driving means After slot, culture dish driving means reversely playbacks, and culture dish is withstood in the rising of culture dish jacking apparatus, and culture dish lockable mechanism unclamps, Culture dish jacking apparatus withstands plate and slowly declines playback, and any 90mm plate can all use on this device.
Plate push and buckle closure device of uncapping are located at the outside of untreated culture dish position in storehouse, have be connected on substrate by electricity The culture dish turnover mechanism that machine drives, culture dish turnover mechanism, which is provided with sucker and can culture dish is sucked or is vented, to fall off.Culture Ware turnover mechanism the inverted untreated culture dish bottom for pushing to groove is sucked and overturn 180 ° it is to be seeded to vaccination station etc..
When work: the untreated culture dish storage inversion plate of position bottom first is pushed to groove by culture dish driving means Interior, the bottom of culture dish is sucked 180 ° of overturning and is just placed on vaccination station by culture dish turnover mechanism, while the sucker in vaccination station is inhaled Firmly culture dish afterwards turns over the exhaust of mechanism sucker and falls off, at this moment cultivates ware lid and stay in groove, culture medium is just being placed on vaccination station etc. It is to be seeded;After crossing, culture dish bottom is sucked 180 ° of overturning to culture dish and covered by the sucker of culture dish turnover mechanism Side, sucker exhaust, culture medium, which is fallen to, to be covered, to complete reversed playback.
Vaccination station: culture dish vaccination station lower part is equipped with rotating electric machine, and integral installation is on a vaccination station support frame.Inoculation Platform support frame is mounted on horizontal conveyor, and vaccination station is moved left and right along horizontal conveyor driven by the motor.
When work: the culture dish uncapped just is put in vaccination station, and oese is gone in the drive backspin for rotating horizontally support base Above vaccination station, the fixed device of oese favours on culture medium in the whole inoculation ring position of drive backspin modulation of rotating electric machine Side.When inoculation, rotating horizontally support base makes oese Y-axis arcuate movement in the horizontal direction on culture medium;Vaccination station is under it Make culture medium along X-axis or so back and forth movement under the horizontal conveyor effect of side, drawing for sample is realized in the superposition of both sides' motion profile Line inoculation.Behind a streak inoculation complete region, vaccination station makes culture medium change an angle, then under rotating electric machine effect The streak inoculation in another region is realized according still further to above-mentioned streak inoculation mode.3 rides, 4 can be realized by above-mentioned vaccination ways The different hatched manners such as ride, the scribing line of rich word and spiral scribing line.
Automatic labeling device includes label absorption panel, vertical transport and horizontal transport mechanism, label adsorption plane Plate is realized lifting by vertical transport and horizontal transport mechanism control and is moved left and right;Label adsorbs panel and is equipped with stomata, Label absorption panel is connected to suction end and the outlet side of vacuum pump.
The work of automatic labeling device is to be located at label machine paper delivery approximately as the initial position of: label absorption panel The top of mouth, the label printed from label machine is smooth up, and viscose is face-down, at this moment opens vacuum pump, vacuum Pump suction end, which generates negative pressure, is attracted to label on label absorption panel, and then label adsorbs panel in horizontal transport mechanism control It is moved back at labeling in the horizontal direction under system, is at this moment inverted plate by transport mechanism and is sent to label absorption below the panel, Label adsorbs panel and moves downward completion labeling movement by vertical transport drive.
The bottom in processed culture dish storehouse is connected with rotating electric machine, and circle distribution has a certain number of culture dish storehouses thereon Storage space, the store in a warehouse bottom of position of culture dish have a circle card along with support culture dish, and culture dish storage is symmetrically installed with 3 trainings on position Ware non-return supporting plate is supported, culture dish non-return supporting plate is rotatablely connected and can only be rotated up, the distance between culture dish supporting plate and card edge More than or equal to the thickness of a culture dish.Side at labeling is provided with the culture dish positioned at culture dish storage position lower part Jacking apparatus I, the culture dish jacking apparatus I is by vertical transport (vertical electric pole) and a culture dish plate being mounted above Composition, culture dish jacking apparatus I are mounted on culture dish driving means I.
When work: after the completion of culture dish labeling, culture dish driving means I pushes culture dish and stores in a warehouse to processed culture dish On position, then culture dish jacking apparatus I moves upwards under the action of vertical electric pole, and culture dish is pushed into three culture dish supporting plates Top, then culture dish jacking apparatus I starts to move downward, and culture dish can be held by three culture dish supporting plates.
The beneficial effects of the present invention are: high-effective microorganism system for pretreating sample is using mechanical arm instead of traditional-handwork method Sample process and streak inoculation carry out screw capping to micro-biological samples automatically, barcode scanning, patch code, carry, uncap, upper cover, streak inoculation Equal sequence of operations, can greatly ensure the bio-safety of microorganism staff, improve work efficiency simultaneously.
Detailed description of the invention
Attached drawing 1 is schematic perspective view one of the invention;
Attached drawing 2 is schematic perspective view two of the invention;
Attached drawing 3 is overlooking structure diagram of the invention;
Attached drawing 4 is schematic view of the front view of the invention;
Attached drawing 5 is left view structural representation of the invention;
Attached drawing 6 is the structural schematic diagram of collet in the present invention;
Attached drawing 7 is untreated culture dish position in storehouse overlooking structure diagram in the present invention;
Attached drawing 8 is the structural schematic diagram of plate lockable mechanism in the present invention;
Attached drawing 9 is the overlooking structure diagram in processed culture dish storehouse in the present invention;
Attached drawing 10 is the structural schematic diagram of culture dish supporting plate in the present invention;
In figure, 1-1 shaking table, 1-2 pore, 2-1 mechanical arm, 2-2 collet, 2-3 sample pipe clip, 2-4 sample cell temporary storage box, 2- 5 scanning machines, 3 high-temperature electromagnetic sterilizers, 4-1 vertical transport, 4-2 rotate horizontally support base, 4-3 horizontal transport mechanism, 4-4 Oese fixes device, 4-5 rotating electric machine, 4-6 oese, 5 untreated culture dish storehouses, 5-1 untreated culture dish storage position, 5- 2 card edges, 5-3 culture dish jacking apparatus, 5-4 culture dish driving means, 5-5 plate lockable mechanism, 6 culture dish turnover mechanisms, 6-1 Sucker, 7 grooves, 8 vaccination stations, 8-1 horizontal conveyor, 9 automatic labeling devices, 9-1 vertical transport, 9-2 label are inhaled Attached panel, 9-3 horizontal transport mechanism, 9-4 label machine, 10 processed culture dish storehouses, 10-1 culture dish non-return supporting plate, 11-1 Culture dish driving means I, 11-2 culture dish jacking apparatus I.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below.
Embodiment
As shown in figures 1-5, the embodiment include the sample bit with shaking table, sample tube clamping and capping device of uncapping, It is disinfector, plane motion classification inoculation apparatus, untreated plate storehouse, plate push and buckle closure device of uncapping, vaccination station, automatic Device for labeling, processed plate storehouse.
Wherein, sample bit includes the shaking table 1-1 in base plate bottom installation, has a certain number of pore 1-2 on the shaking table And the pore size of each pore and sample tube match and are used to place sample tube.
Sample to be examined pipe is placed in the pore 1-2 of shaking table 1-1, after instrument starting, sample in sample tube is with shaking Bed concussion, can promote the homogenize process of sample, improves the recall rate of sample.
Sample tube clamping and capping device of uncapping include the mechanical arm 2-1 positioned at sample bit side, and mechanical arm 2-1 is X 3 shaft mechanical arms of axis, Y-axis and Z-direction transmission, be equipped on mechanical arm 2-1 realization sample tube is clamped, uncap and The collet 2-2 of upper cover operation;Collet is mounted on mechanical arm 2-1 by rotating electric machine, as shown in Figure 6, the tube wall of collet Inner periphery is disposed with the spring clip that can be fastened on sample tube lid, and the bottom end of spring clip is provided with chamfering to facilitate sample The importing of this pipe lid, the inner surface of spring clip have vertical card line with the vertical line clamping with sample tube exterior surface.When clamping: machine Tool arm 2-1 control collet is moved to above sample tube and moves down, and the lid of sample tube can be packed into collet by pressure Spring clip in, realize clamping to sample tube;When uncapping: it is rotated counterclockwise by controlling rotating electric machine, it can be by sample Pipe Gai Xuankai;When upper cover, lid is pressed into the nozzle of sample tube by mechanical arm control collet, and rotating electric machine, which rotates clockwise, to be covered Son, which screws, completes upper cover, and the collet of mechanical arm control later moves up, and makes spring clip and sample tube lid using upward pulling force It is detached from.
Sample tube clamping and capping device of uncapping further include that the sample tube positioned at sampling position and for fixed sample pipe is fixed Press from both sides 2-3.
After instrument starting, collet 2-2 by 3 shaft mechanical arm 2-1 and thereon realizes the clamping and transhipment of sample tube, I.e. by the sample tube (position of collet crawl is sample tube lid) on collet 2-2 crawl sample bit, and pass through mechanical arm 2-1 for sample This pipe is transferred to sampling position by sample bit, then is clamped fixation to sample tube by the sample pipe clip 2-3 on sampling position;It The collet 2-2 on mechanical arm is rotated counterclockwise by rotating electric machine and unscrews the lid of sample tube afterwards, and the lid after unscrewing passes through Mechanical arm is moved to side;After to be sampled, mechanical arm 2-1 control collet 2-2 is moved to above sample tube, is then transported downwards Dynamic, when lid is pressed against the nozzle of sample tube, rotating electric machine driving collet 2-2 rotation completes upper cover to screw lid, The collet 2-2 of mechanical arm control later, which is moved upwards, is detached from sample tube lid.After upper cover, sample pipe clip 2-3 unclamps, and sample tube is fallen Enter in sample tube temporary storage box 2-4.
1 to 6 kind of different color can be set into sample tube cover in above scheme, indicates different types of sample, leads to It crosses pipe upper part of the cover camera and screens different colors, and the culture dish of corresponding different untreated culture dish storage position 5-1.
Scanning machine 2-5 is equipped on the outside of above-mentioned sample tube clamping and capping device of uncapping, barcode scanning personal information is simultaneously transferred to certainly At dynamic device for labeling.
Disinfector is high-temperature electromagnetic sterilizer 3.
Plane motion classification inoculation apparatus includes the horizontal rotation support base 4-2 motor-driven being connected with substrate, horizontal The upper end rotary support seat 4-2 is installed by the vertical electric pole of vertical transport 4-1(), horizontal conveyor is installed in vertical transport Oese fixed device 4-4 is installed on structure 4-3, horizontal transport mechanism 4-3, sets rotating electric machine in the fixed device 4-4 of oese 4-5 drives the position backspin modulation whole oese 4-6 in rotating electric machine 4-5.
When work: the horizontal rotation support base 4-2 being connected with substrate rotates oese 4-6 to high-temperature electromagnetic sterilizer 3 Top, oese 4-6 is inserted perpendicularly into high-temperature electromagnetic sterilizer 3 sterilization that carries out disinfection by vertical transport 4-1, to be sterilized to kill After bacterium, vertical transport 4-1 moves upwards extraction oese.By rotating horizontally the rotation of support base 4-2, will be inoculated with Ring 4-6 is rotated to the sample tube uncapped, and is moved down and is dipped sample.Support base 4-2 will be rotated horizontally after the completion of sampling to be inoculated with Ring 4-6 is rotated to vaccination station 8.
The bottom of untreated culture dish position in storehouse 5 is connected with rotating electric machine, and circle distribution has and a certain number of do not locate thereon Culture dish storage position 5-1 is managed, bottom has a circle card along 5-2 with support culture dish;Side close to vaccination station is provided with position In the culture dish jacking apparatus 5-3 of the culture dish storage bottom surface position 5-1 and the culture dish being mounted below untreated culture dish position in storehouse 5 Driving means 5-4, the culture dish jacking apparatus 5-3 are by vertical transport (vertical electric pole) and a culture being mounted above Ware supporting plate composition, culture dish jacking apparatus 5-3 is mounted on culture dish driving means 5-4 by horizon bar, when not working, culture Ware plate coincides with card along 5-2;3 culture dish lockable mechanisms have been arranged symmetrically on the storage position 5-1 in untreated culture dish storehouse 5-5, culture dish lockable mechanism is more than or equal to the thickness of a culture dish at a distance from culture dish card edge, should as shown in Fig. 7,8 Culture dish lockable mechanism is to push tapered end Forward to realize the locking to culture dish by screw drive mode by motor.
When work: by culture dish upside down in untreated culture dish storage position 5-1, untreated culture dish storage position 5-1 It is rotated under the rotating electric machine effect of its bottom, the untreated culture dish storage position 5-1 that will be inoculated with is rotated to close to vaccination station Side.Culture dish vertically moves upward to certain position, culture dish lock under the action of culture dish jacking apparatus 5-3 Locking mechanism 5-5 locks the plate of bottom second, and culture dish jacking apparatus 5-3 starts slowly to decline playback immediately, culture dish push After the plate of bottom first is released culture dish storage position to groove 7 by device 5-4, culture dish driving means 5-4 reversely playbacks, training It supports ware jacking apparatus 5-3 rising and withstands culture dish, culture dish Suo Zhi mechanism 5-5 unclamps, and culture dish jacking apparatus 5-3 withstands plate Slowly decline playback, any 90mm plate can all use on this device.
Plate push and buckle closure device of uncapping are located at the outside of untreated culture dish position in storehouse 5, have be connected on substrate by Motor-driven culture dish turnover mechanism 6, culture dish turnover mechanism 6, which is provided with sucker and can culture dish is sucked or is vented, to fall off. Culture dish turnover mechanism 6 will push to the inverted culture dish bottom of groove 7 and be sucked and overturn 180 ° (to be connect to vaccination station etc. is to be seeded The table top of kind platform is located at the centre of turnover mechanism, and the two is non-interference).
When work: culture dish driving means 5-4 pushes the untreated culture dish storage bottom position 5-1 first inversion plate To groove 7, the bottom of culture dish is sucked 180 ° of overturning and is just being placed in vaccination station 8 by culture dish turnover mechanism 6, in vaccination station 8 Sucker culture dish is sucked, then 6 sucker of culture dish turnover mechanism exhaust fall off, at this moment cultivate ware lid stay in groove 7, cultivate It is to be seeded that base stays in vaccination station 8 etc.;After crossing, overturning is sucked in culture dish bottom by the sucker of culture dish turnover mechanism 6 180 ° are vented to the culture dish side of covering, sucker, and culture medium, which is fallen to, to be covered, to complete reversed playback.
Vaccination station: 8 bottom of culture dish vaccination station is equipped with rotating electric machine, and integral installation is on a vaccination station support frame.It connects Kind platform support frame is mounted on horizontal conveyor 8-1, and culture dish vaccination station 8 is along horizontal conveyor 8- driven by the motor 1 moves left and right.
When work: after the culture dish uncapped just is being put in vaccination station 8, plane motion classification inoculation apparatus is rotated to 8 top of vaccination station, Oese 4-6 is favoured above culture medium under the fixed device 4-4 and rotating electric machine 4-5 effect of oese.When inoculation, oese In the case where rotating horizontally support base 4-2 and driving along Y-axis arcuate movement, the horizontal conveyor of vaccination station thereunder on culture medium It is moved back and forth under 8-1 effect along X-axis or so, the streak inoculation of sample is realized in the superposition of both sides' motion profile.When streak inoculation complete one Behind a region, vaccination station 8 makes culture medium change an angle, then according still further to above-mentioned streak inoculation side under rotating electric machine effect Formula realizes the streak inoculation in another region.3 rides, 4 rides, the scribing line of rich word and spiral can be realized by above-mentioned vaccination ways The different hatched manners such as scribing line.
Automatic labeling device 9 includes that label adsorbs panel 9-2, vertical transport 9-1 and horizontal transport mechanism 9-3, Label, which adsorbs panel 9-2, to be realized lifting by vertical transport 9-1 and horizontal transport mechanism 9-3 control and is moved left and right;Label It adsorbs panel 9-2 and is equipped with stomata, label absorption panel 9-2 is connected to suction end and the outlet side of vacuum pump.
The work of automatic labeling device is to be located at label machine approximately as the initial position of: label absorption panel 9-2 The top of 9-4 exit slot, the label printed from label machine 9-4 is smooth, and viscose is face-down up, at this moment opens true Sky pump, vacuum pump suction end, which generates negative pressure, is attracted to label on label absorption panel 9-2, and then label adsorbs panel 9-2 It is moved back at labeling in the horizontal direction under horizontal transport mechanism 9-3 control, is at this moment inverted plate and is sent to label adsorption plane Below plate 9-2, label adsorbs panel 9-2 and moves downward completion labeling movement by vertical transport drive.
The bottom in processed culture dish storehouse 10 is connected with rotating electric machine, and circle distribution has a certain number of culture dishes thereon It stores in a warehouse position, the store in a warehouse bottom of position of culture dish has a circle card along with support culture dish, and culture dish storage is symmetrically installed with 3 on position Culture dish non-return supporting plate 10-1, as shown in Fig. 9,10, culture dish non-return supporting plate 10-1 is rotatablely connected and can only be rotated up, and trains Support the thickness that the distance between ware non-return supporting plate 10-1 and card edge are more than or equal to a culture dish.It is set in the side at labeling It is equipped with I 11-2 of culture dish jacking apparatus for being located at culture dish storage position lower part, I 11-2 of the culture dish jacking apparatus is by vertically transmitting Mechanism (vertical electric pole) and a culture dish board group being mounted above are at I 11-2 of culture dish jacking apparatus is mounted on culture dish On I 11-1 of driving means.
When work: after the completion of culture dish labeling, I 11-1 of culture dish driving means pushes culture dish to processed culture dish It stores in a warehouse on position, then I 11-2 of culture dish jacking apparatus is moved upwards under the action of vertical electric pole, and culture dish is pushed into 3 trainings The top ware non-return supporting plate 10-1 is supported, then I 11-2 of culture dish jacking apparatus starts to move downward, and culture dish can be by 3 culture dishes Non-return supporting plate 10-1 is held.
Finally, it is stated that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although referring to compared with Good embodiment describes the invention in detail, those skilled in the art should understand that, it can be to skill of the invention Art scheme is modified or replaced equivalently, and without departing from the objective and range of technical solution of the present invention, should all be covered at this In the scope of the claims of invention.

Claims (14)

1. a kind of untreated culture dish storehouse is used for micro-biological samples preprocessing system, stores untreated culture dish and gone out Storehouse control, it is characterised in that: the bottom in the untreated culture dish storehouse is connected with rotating electric machine, and circle distribution has centainly thereon The culture dish storage position of quantity, the bottom of culture dish storage position have a circle card edge with support culture dish;It is provided in export side Culture dish jacking apparatus and culture dish driving means below culture dish storage position, culture dish jacking apparatus are mounted on culture On ware driving means, the culture dish jacking apparatus carries out elevating control to culture dish, and the culture dish driving means will be cultivated Ware pushes export by storage position;Culture dish storage is disposed with culture dish lockable mechanism, culture dish lockable mechanism and culture dish on position The distance on storage position bottom card edge is more than or equal to the height of a culture dish.
2. a kind of plate push and buckle closure device of uncapping, are used for micro-biological samples preprocessing system, inverted culture dish are turned over Turn and be placed in vaccination station, it is characterised in that: the plate push and buckle closure device of uncapping are located at going out for untreated culture dish storehouse , there is culture dish turnover mechanism motor-driven in storehouse side, and culture dish turnover mechanism is provided with sucker and can culture dish is sucked or be arranged Qi exhaustion is fallen;The inverted culture dish bottom that untreated culture dish storehouse export side is pushed out is sucked simultaneously the culture dish turnover mechanism Just be put in vaccination station after 180 ° of overturning, it is waiting finish after, culture dish bottom is sucked and is overturn again by the culture dish turnover mechanism 180 ° of playback buckle closures.
3. a kind of processed culture dish storehouse, it is used for micro-biological samples preprocessing system, processed culture dish is put in storage storage, Be characterized in that: the bottom in processed culture dish storehouse is connected with rotating electric machine, and circle distribution has a certain number of culture dishes thereon Storage position, the bottom of culture dish storage position have a circle card edge with support culture dish;Side at labeling is provided with position Culture dish jacking apparatus I and culture dish driving means I in culture dish storage position lower part, the culture dish jacking apparatus I are installed On culture dish driving means I, the culture dish jacking apparatus I carries out elevating control, the culture dish push dress to culture dish It sets I and culture dish is pushed into go back to storage position by labeling;The culture dish storage is disposed with the culture dish being unidirectionally rotated up on position Non-return supporting plate, the distance between culture dish supporting plate and card edge are more than or equal to the thickness of a culture dish.
4. a kind of high-effective microorganism system for pretreating sample characterized by comprising sample bit, sample pipe clamp with shaking table Take and uncap capping device, disinfector, plane motion classification inoculation apparatus, untreated culture dish storehouse, plate push and uncap Buckle closure device, vaccination station, automatic labeling device, processed culture dish storehouse.
5. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the sample bit include The shaking table of base plate bottom installation, pore size and sample tube phase on the shaking table with a certain number of pores and each pore Match.
6. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the sample tube is clamped and opened Covering lid arrangement includes the mechanical arm positioned at sample bit side, and realization is equipped on the mechanical arm and is pressed from both sides to sample tube Take, uncap and upper cover operation collet;The sample tube clamping and capping device of uncapping further include having to be used in combination positioned at sampling position Sample pipe clip in fixed sample pipe and the scanning machine outside capping device of uncapping;Sample fixing clamp lower part is equipped with Sample cell temporary storage box.
7. high-effective microorganism system for pretreating sample according to claim 6, it is characterised in that: the collet is to pass through rotation Motor is installed on the robotic arm, and the spring clip that can be fastened on sample tube lid is evenly equipped in the tube wall of collet.
8. high-effective microorganism system for pretreating sample according to claim 6, it is characterised in that: the pipe lid of the sample tube is set 1 to 6 kind of different color is set to indicate different types of sample, different face can be screened by pipe upper part of the cover camera Color, and the culture dish of corresponding different untreated culture dish storage positions.
9. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the plane motion inoculation dress Setting includes the horizontal rotation support base being connected with substrate, rotates horizontally support base upper end and is equipped with vertical biography motor-driven Mechanism is sent, horizontal transport mechanism is installed in vertical transport, oese fixed device is installed in horizontal transport mechanism, is connect Rotating electric machine is set in the fixed device of kind ring, drives the whole inoculation ring position of backspin modulation in rotating electric machine.
10. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the untreated plate storehouse The bottom of position is connected with rotating electric machine, and circle distribution has a certain number of culture dish storages position thereon, culture dish storage position Bottom has a circle card along with support culture dish;Side close to vaccination station is provided with the training below culture dish storage position Support ware jacking apparatus and the culture dish driving means that is mounted below untreated culture dish position in storehouse, the culture dish jacking apparatus by Vertical transport and the culture dish supporting plate being mounted above form, and culture dish jacking apparatus is mounted on culture dish driving means On;Untreated culture dish storage is disposed with culture dish lockable mechanism on position, and culture dish lockable mechanism is at a distance from culture dish card edge More than or equal to the height of a culture dish.
11. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the plate is pushed and opened Cover buckle lid arrangement is located at the outside of untreated culture dish position in storehouse, there is the culture dish tipper motor-driven being connected on substrate Structure, culture dish turnover mechanism, which is provided with sucker and can culture dish is sucked or is vented, to fall off;Plate pushes and uncap buckle closure device will Inverted untreated culture dish is shifted onto groove, and culture dish bottom is sucked culture dish turnover mechanism overturns 180 ° to vaccination station Etc. to be seeded.
12. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the vaccination station lower part peace Equipped with rotating electric machine, integral installation is on a vaccination station support frame, and vaccination station support frame is mounted on horizontal conveyor, in electricity Move back and forth culture medium inoculated platform along horizontal conveyor or so, the mobile oese with arcuately direction The streak inoculation of sample is realized in track superposition.
13. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: the automatic labeling device It include label absorption panel, vertical transport and horizontal transport mechanism, the label absorption panel is by vertical transport It controls realization lifting with horizontal transport mechanism and moves left and right and adsorbed and moved at labeling with the label for exporting label machine It is affixed on culture dish bottom;Label adsorbs panel and is equipped with stomata, and label absorption panel is connected to the suction end and outlet of vacuum pump End.
14. high-effective microorganism system for pretreating sample according to claim 4, it is characterised in that: processed culture dish storehouse Bottom is connected with rotating electric machine, and circle distribution has a certain number of culture dish storages position, the bottom of culture dish storage position thereon With a circle card along with support culture dish, culture dish storage is disposed with culture dish non-return supporting plate on position, and culture dish non-return supporting plate turns Dynamic to connect and be rotated up, the distance between culture dish supporting plate and card edge are more than or equal to the thickness of a culture dish;It is leaning on Side at nearly labeling is provided with the culture dish jacking apparatus I positioned at culture dish storage position lower part, the culture dish jacking apparatus I by Vertical transport and a culture dish board group being mounted above are at culture dish jacking apparatus I is mounted on culture dish push dress It sets on I.
CN201910757355.8A 2019-08-16 2019-08-16 A kind of high-effective microorganism system for pretreating sample Withdrawn CN110484437A (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175811A (en) * 2020-10-13 2021-01-05 上海莫杜生物科技有限公司 Full-automatic microbial inoculation appearance
CN112662523A (en) * 2021-02-08 2021-04-16 济南百博生物技术股份有限公司 Automatic scribing device and scribing method for microbial sample pretreatment system
CN112708543A (en) * 2021-01-08 2021-04-27 湖南隋侯珠科技有限公司 Inoculating loop
CN112725168A (en) * 2021-01-08 2021-04-30 湖南隋侯珠科技有限公司 Automatic processing system for microbial samples
CN113337389A (en) * 2021-07-12 2021-09-03 安图实验仪器(郑州)有限公司 Full-automatic microbial sample pretreatment system
CN114276904A (en) * 2022-01-24 2022-04-05 深圳先进技术研究院 Culture dish filling equipment
CN117363469A (en) * 2023-12-08 2024-01-09 成都佳仕得科技有限公司 High-precision inoculation device and method for continuously inoculating microorganisms

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112175811A (en) * 2020-10-13 2021-01-05 上海莫杜生物科技有限公司 Full-automatic microbial inoculation appearance
CN112175811B (en) * 2020-10-13 2022-11-11 上海莫杜生物科技有限公司 Full-automatic microbial inoculation appearance
CN112708543A (en) * 2021-01-08 2021-04-27 湖南隋侯珠科技有限公司 Inoculating loop
CN112725168A (en) * 2021-01-08 2021-04-30 湖南隋侯珠科技有限公司 Automatic processing system for microbial samples
CN112662523A (en) * 2021-02-08 2021-04-16 济南百博生物技术股份有限公司 Automatic scribing device and scribing method for microbial sample pretreatment system
CN113337389A (en) * 2021-07-12 2021-09-03 安图实验仪器(郑州)有限公司 Full-automatic microbial sample pretreatment system
CN114276904A (en) * 2022-01-24 2022-04-05 深圳先进技术研究院 Culture dish filling equipment
CN117363469A (en) * 2023-12-08 2024-01-09 成都佳仕得科技有限公司 High-precision inoculation device and method for continuously inoculating microorganisms
CN117363469B (en) * 2023-12-08 2024-02-06 成都佳仕得科技有限公司 High-precision inoculation device and method for continuously inoculating microorganisms

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