CN216838006U - Full-automatic cell liquid changing device - Google Patents
Full-automatic cell liquid changing device Download PDFInfo
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- CN216838006U CN216838006U CN202122907345.3U CN202122907345U CN216838006U CN 216838006 U CN216838006 U CN 216838006U CN 202122907345 U CN202122907345 U CN 202122907345U CN 216838006 U CN216838006 U CN 216838006U
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Abstract
The utility model discloses a full-automatic cell liquid changing device, which comprises: fixing the platform; the transferring mechanical arm is used for the culture dish body and is arranged on the fixed platform; the liquid changing mechanism is arranged on the fixed platform and comprises a needle head module and a moving driving mechanism for moving the needle head module, the needle head module comprises a fixed block, a liquid adding needle tube and a liquid absorbing needle tube, the liquid adding needle tube and the liquid absorbing needle tube are fixed on the fixed block, and the liquid absorbing needle tube is provided with a liquid absorbing needle; the culture dish workbench is used for placing the culture dish body. The utility model provides a full-automatic cell liquid changing device, which realizes full-automatic liquid changing in the whole liquid changing process and reduces the operation error and pollution risk caused by manual operation.
Description
Technical Field
The utility model relates to the technical field of cell liquid changing equipment, in particular to a full-automatic cell liquid changing device.
Background
At present, most cell preparation operations are performed in an open cell laboratory, manual operations are mainly used, and cell preparation personnel need to be trained. The quality of cell preparation is affected by the problems of pollution risk, poor reproducibility and the like caused by manual operation. In addition, the manual operation is inefficient, and the liquid change is usually performed many times in the whole cell preparation cycle, which requires a lot of manual work to complete the work, and this is also one of the reasons for the high cost of cell preparation. If the automation can be used for replacing the manual work in the cell liquid changing process, the cell liquid changing pollution risk is reduced, and the liquid changing efficiency is greatly improved. Therefore, a full-automatic cell liquid changing device is needed to be provided, so that full-automatic liquid changing is realized in the whole liquid changing process, and the operation errors and the risks of pollution caused by manual operation are reduced; the liquid suction needle head can be automatically replaced, so that the automation degree is further improved, and cross contamination is avoided; simultaneously, make the imbibition syringe needle absorb the waste liquid totally through the structure setting.
Chinese patent application publication No. CN113025482A, published as 2021, 06, 25, entitled "a full-automatic liquid-changing system", discloses a full-automatic liquid-changing system for adding or absorbing liquid to a cell storage container; the method comprises the following steps: the device comprises a screw capping device, a liquid suction device, a liquid feeding device and a weighing platform which are arranged in sequence; wherein, the screwing device is used for screwing the cell storage container cover open or screwed; the liquid suction device comprises a liquid suction needle head, a liquid suction pipe, a liquid suction peristaltic pump and a waste liquid storage which are sequentially communicated so as to suck out liquid in the cell storage container; the liquid feeding device comprises a liquid feeding needle head, a liquid feeding pipe, a liquid feeding peristaltic pump and a liquid feeding storage mechanism which are sequentially communicated, and the liquid feeding needle head, the liquid feeding pipe, the liquid feeding peristaltic pump and the liquid feeding storage mechanism are used for adding culture solution into the cell storage container. However, the liquid changing system can only change liquid for the cell storage container placed on the platform, the cell storage container cannot be automatically changed, and the continuous liquid changing operation can be completed only by matching the manual work and external equipment. And the imbibition syringe needle in this patent can't be changed automatically, causes cross contamination easily, influences cell culture's quality, does not set up the structure that makes the imbibition more thorough yet, leads to having the incomplete problem of imbibition.
Disclosure of Invention
The utility model provides a full-automatic cell liquid changing device, aiming at overcoming the defects that the cell preparation operation is mostly carried out in an open cell laboratory in the prior art, the manual operation is mainly used, the quality of cell preparation is influenced by the problems of pollution risk, poor reproducibility and the like caused by the manual operation, the full-automatic cell liquid changing device is provided, the full-automatic liquid changing is realized in the whole liquid changing process, and the operation error and the pollution risk caused by the manual operation are reduced.
In order to achieve the purpose, the utility model adopts the following technical scheme:
a fully automated cell exchange solution device, comprising:
fixing the platform;
the transfer manipulator is used for the culture dish body and is arranged on the fixed platform;
the liquid changing mechanism is arranged on the fixed platform and comprises a needle head module and a moving driving mechanism used for moving the needle head module, the needle head module comprises a fixed block, a liquid adding needle tube and a liquid absorbing needle tube, the liquid adding needle tube and the liquid absorbing needle tube are fixed on the fixed block, and the liquid absorbing needle tube is provided with a liquid absorbing needle;
the culture dish worktable is used for placing the culture dish body.
In the technical scheme, the liquid suction needle tube is communicated with an external liquid suction device and can suck waste liquid; the liquid feeding needle tube is communicated with an external cell culture solution conveying mechanism, and can provide required culture solution. The working process of the technical scheme is as follows: can take out the culture dish body in the incubator through transporting the manipulator to place the culture dish body on the culture dish workstation, stretch into the culture dish body through liquid changing mechanism with the imbibition syringe needle and carry out the imbibition, the imbibition is accomplished the back, stretches into culture dish body with the liquid feeding needle tubing and adds the culture solution, then sends the culture dish body back to the incubator through transporting the manipulator. The whole liquid changing process in the technical scheme realizes full-automatic liquid changing, and reduces operation errors and pollution risks caused by manual operation.
Preferably, the transferring manipulator comprises a translation mechanism, a lifting mechanism, a rotating mechanism and a transferring plate, the transferring plate is fixed on the rotating mechanism, the rotating mechanism is arranged on the lifting mechanism, and the lifting mechanism is fixed on the translation mechanism. The translation mechanism comprises a slide rail and a linear driver, the linear driver can be an air cylinder, an oil cylinder, a push rod motor, a screw rod nut mechanism driven by a motor, a gear rack mechanism driven by the motor and the like, and the gear rack mechanism driven by the motor is selected; the lifting mechanism can be a cylinder, an oil cylinder, a push rod motor, a screw rod nut mechanism driven by a motor, a gear rack mechanism driven by the motor and the like, wherein the gear rack mechanism driven by the motor is selected; the rotating mechanism is a motor. The manipulator sideslip, lift and pivoted action needs can be realized transporting to the structure, and the transportation board is used for holding up the culture dish body.
Preferably, the movement driving mechanism comprises an X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, the needle module is fixed on the Z-axis moving mechanism, the Z-axis moving mechanism is fixed on the Y-axis moving mechanism, and the Y-axis moving mechanism is fixed on the X-axis moving mechanism. The X-axis moving mechanism, the Y-axis moving mechanism and the Z-axis moving mechanism can be cylinders, oil cylinders, push rod motors, motor-driven lead screw and nut mechanisms, motor-driven gear and rack mechanisms and the like, and the motor-driven lead screw and nut mechanisms are selected. The structure can realize the translation of the movement driving mechanism in the three directions of the X axis, the Y axis and the Z axis, thereby driving the needle head module to move as required.
Preferably, a needle placing frame is arranged on the fixing platform, and a plurality of unused liquid suction needles are arranged on the needle placing frame. A plurality of unused liquid suction needles are arranged in order. When being equipped with a plurality of hole sites on the culture dish body, in order to avoid the mutual pollution between every hole site, the waste liquid in every hole site absorbs the back, needs to change the imbibition syringe needle, and syringe needle puts the frame and is used for placing the imbibition syringe needle that does not use. And positioning columns are arranged around the needle head placing frame and are fixed with the fixing platform.
Preferably, a clamping ring is arranged on the outer side wall of the liquid suction needle tube, and the liquid suction needle tube is clamped with a liquid suction needle head on the liquid suction needle tube through the clamping ring. The structure makes things convenient for imbibition needle tubing and imbibition syringe needle joint, and imbibition syringe needle itself has certain elasticity, can block the imbibition syringe needle through the snap ring.
Preferably, the fixing platform is provided with a waste needle collecting box, a needle releasing block is fixed above the waste needle collecting box, and the needle releasing block is provided with a limiting groove matched with the liquid suction needle. And a positioning column is arranged around the waste liquid discharge barrel and is fixed with the fixing platform. The structure can be used for making the imbibition syringe needle break away from the imbibition needle tubing, and the imbibition syringe needle needs to be changed after having absorbed the waste liquid, removes actuating mechanism and removes the imbibition syringe needle to spacing groove below, then upwards lifting syringe needle module, makes and takes off the upper end that the needle piece blocked the imbibition syringe needle, makes the imbibition syringe needle break away from the imbibition needle tubing, falls into in the abandonment syringe needle collection box. The reference column can be fixed a position and fix abandonment syringe needle collection box.
Preferably, the upper end of the liquid suction needle head is provided with a limiting ring, and the outer diameter of the limiting ring is larger than the width of the limiting groove. The limiting ring facilitates the liquid suction needle head to be separated from the liquid suction needle tube.
Preferably, the lower end of the liquid adding needle tube is provided with a liquid adding needle head.
Preferably, the lower end of the filling needle is bent to one side. The structure can make the liquid outlet of the liquid feeding needle close to the side wall of one side of the culture medium, so that the cell culture solution slowly flows downwards along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast.
Preferably, a culture dish shifting sheet is fixed on the fixing block and used for transversely shifting the culture dish body on the culture dish workbench. The culture dish plectrum can be with transporting the culture dish body on the manipulator and moving to the culture dish workstation on.
Preferably, the culture dish workstation is fixed with fixed platform including the fixing base and the rotation seat that set up side by side, rotates the seat and rotates with fixed platform and be connected, rotates the axis of rotation and the horizontal plane parallel arrangement of seat, and fixed platform is last to be equipped with the drive and rotates a pivoted rotation driver, and during the liquid change, the culture dish body is placed on rotating the seat.
Among the above-mentioned technical scheme, after the transfer manipulator takes out the culture dish body in with the incubator, place on the fixing base, then the culture dish plectrum can move the culture dish body to on rotating the seat, the rethread rotates the driver and makes and rotate the seat and rotate the slope, make the slope of culture dish body, the imbibition syringe needle of being convenient for absorbs the waste liquid totally, then rethread liquid feeding needle tube carries out the liquid feeding, the liquid feeding is accomplished the back, it makes and rotates the seat and rotate the repayment, the culture dish plectrum can be with on the culture dish body moves the fixing base, can send the culture dish body back to the incubator through the transfer manipulator.
Preferably, the fixing base is provided with an accommodating groove for accommodating a transfer plate of the transfer manipulator. The holding tank is convenient for transport the manipulator and is stretched into between fixing base and the culture dish body.
Preferably, the rotating seat is eccentrically arranged below the rotating seat, the rotating driver comprises an electromagnet, the electromagnet is fixed below the rotating seat, the rotating shaft is arranged between the gravity center of the rotating seat and the electromagnet, a magnetic suction block is arranged at the position, corresponding to the electromagnet, of the lower end of the rotating seat, and when the electromagnet is electrified, the electromagnet is in contact with the magnetic suction block. The structure can realize the rotation of the rotating seat, the rotating structure is simple and reliable, the cost is low, the control is simple, and the rotation can be realized only by one electromagnet. And the rotating seat is kept horizontal by the gravity of the rotating seat.
Preferably, an adjusting base is arranged below the rotating base, the adjusting base is fixed with the fixed platform, an adjusting rod which is in threaded connection with the adjusting base and can be adjusted up and down is arranged on the adjusting base, the top end of the adjusting rod is in contact with the bottom end of the rotating base, and the adjusting rod and the electromagnet are arranged at two opposite ends of the rotating shaft. The structure can adjust the horizontal state of the rotating seat through rotating the adjusting rod, so that the equipment can be conveniently adjusted.
Preferably, one end of the rotating seat, which is far away from the fixed seat, is provided with a limiting block for limiting the position of the culture dish body. The structure can be used for positioning the position of the culture dish body and preventing the culture dish body from sliding away in an inclined state.
Preferably, the lower end of the liquid adding needle tube is provided with a liquid adding needle head, and the lower end of the liquid adding needle head is bent towards the side with lower height after inclining towards the rotating seat. The structure can ensure that the liquid outlet of the liquid adding needle head is close to the culture medium and is positioned on the side wall which is relatively below the culture medium, so that the cell culture solution slowly flows downwards along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast.
Preferably, a rotary damper is provided on the rotating shaft. The structure can make the seat that rotates have certain buffering when rotating, avoids rotating too fast and leads to the culture solution to rock and spill.
Preferably, a waste liquid discharge barrel is arranged on the fixed platform. The waste liquid discharge barrel can be used for collecting redundant liquid in the liquid adding needle tube.
Preferably, a positioning column is arranged around the waste liquid discharge barrel and is fixed with the fixing platform. The reference column can be fixed a position and fix waste liquid discharge barrel.
Preferably, the fixed platform is provided with a vacuum chuck and a chuck lifting mechanism, the chuck lifting mechanism is fixed with the fixed platform, the vacuum chuck is fixed with the chuck lifting mechanism, and the vacuum chuck is arranged right above the fixed seat. The structure may be used to suck the lid on the culture dish body.
Preferably, a microscope photographing station is arranged on the fixed platform, and a photographing fixing seat matched with the culture dish body is fixed on the microscope photographing station.
The utility model has the beneficial effects that: (1) the full-automatic liquid changing is realized in the whole liquid changing process, and the operation error and the pollution risk caused by manual operation are reduced; (2) the liquid suction needle head can be automatically replaced; (3) the liquid outlet of the liquid feeding needle head is close to the side wall of one side of the culture medium, so that the cell culture solution slowly flows downwards along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast; (4) the rotating seat is rotated and inclined through the rotating driver, so that the culture dish body is inclined, and the liquid suction needle head can conveniently suck the waste liquid completely; (5) the rotating structure of the rotating seat is simple and reliable, the cost is low, the control is simple, the rotation can be realized only by one electromagnet, and the rotating seat is kept horizontal by utilizing the gravity of the rotating seat.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view showing the structure of a transfer robot in the present invention;
FIG. 3 is a schematic view of a partial structure of the present invention;
FIG. 4 is a schematic view of the construction of the needle module of the present invention;
FIG. 5 is a schematic view showing the structure of a petri dish table in the utility model;
fig. 6 is a schematic view showing the construction of the waste needle collecting container according to the present invention.
In the figure: fixed platform 1, transport manipulator 2, translation mechanism 2.1, elevating system 2.2, slewing mechanism 2.3, transport board 2.4, trade liquid mechanism 3, syringe needle module 3.1, fixed block 3.1.1, liquid feeding needle tube 3.1.2, imbibition needle tube 3.1.3, snap ring 3.1.4, imbibition syringe needle 3.1.5, spacing ring 3.1.6, liquid feeding syringe needle 3.1.7, mobile drive mechanism 3.2, culture dish workstation 4, fixing base 4.1, rotating base 4.2, axis of rotation 4.3, holding tank 4.4, rotation driver 4.5, magnetism piece 4.6, stopper 4.7, culture dish body 5, syringe needle put frame 6, abandonment syringe needle collection box 7, needle removal piece 8, spacing groove 8.1, culture dish plectrum 9, adjusting base 10, adjusting lever 11, waste liquid discharge bucket 12, reference column 13, microscope station 14.
Detailed Description
Example 1:
As shown in fig. 1, a full-automatic cell liquid-changing device comprises a fixed platform 1, a transferring manipulator 2, a liquid-changing mechanism 3, a culture dish workbench 4 and a needle head placing frame 6; the transferring mechanical arm 2 is used for the culture dish body 5, and the transferring mechanical arm 2 is arranged on the fixed platform 1; as shown in fig. 2, the transferring manipulator 2 includes a translation mechanism 2.1, an elevating mechanism 2.2, a rotating mechanism 2.3 and a transferring plate 2.4, the transferring plate 2.4 is fixed on the rotating mechanism 2.3, the rotating mechanism 2.3 is arranged on the elevating mechanism 2.2, the elevating mechanism 2.2 is fixed on the translation mechanism 2.1, the translation mechanism 2.1 and the elevating mechanism 2.2 are gear rack mechanisms driven by a motor, and the rotating mechanism 2.3 is a motor.
As shown in fig. 1 and 3, the liquid changing mechanism 3 is arranged on the fixed platform 1, the liquid changing mechanism 3 comprises a needle module 3.1 and a moving driving mechanism 3.2 for moving the needle module 3.1, as shown in fig. 4, the needle module 3.1 comprises a fixed block 3.1.1, a liquid adding needle tube 3.1.2 and a liquid absorbing needle tube 3.1.3, the liquid adding needle tube 3.1.2 and the liquid absorbing needle tube 3.1.3 are fixed on the fixed block 3.1.1, and the liquid absorbing needle tube 3.1.3 is provided with a liquid absorbing needle 3.1.5; the movement driving mechanism 3.2 comprises an X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, the needle head module 3.1 is fixed on the Z-axis moving mechanism, the Z-axis moving mechanism is fixed on the Y-axis moving mechanism, and the Y-axis moving mechanism is fixed on the X-axis moving mechanism. The X-axis moving mechanism, the Y-axis moving mechanism and the Z-axis moving mechanism adopt a screw rod and nut mechanism driven by a motor. The culture dish worktable 4 is used for placing a culture dish body 5. A culture dish shifting sheet 9 is fixed on the fixed block 3.1.1, and the culture dish shifting sheet 9 is used for transversely shifting the culture dish body 5 on the culture dish workbench 4. The fixed platform 1 is provided with a waste liquid discharge barrel 12, a microscope photographing station 14, a vacuum sucker and a sucker lifting mechanism 2.2. A positioning column 13 is arranged around the waste liquid discharge barrel 12, and the positioning column 13 is fixed with the fixed platform 1. A photographing fixing seat 4.1 matched with the culture dish body 5 is fixed on the microscope photographing station 14. The sucker lifting mechanism 2.2 is fixed with the fixed platform 1, the vacuum sucker is fixed with the sucker lifting mechanism 2.2, and the vacuum sucker is arranged right above the fixed seat 4.1.
In the technical scheme, the liquid suction needle tube 3.1.3 is communicated with an external liquid suction device and can suck waste liquid; the liquid adding needle tube 3.1.2 is communicated with an external cell culture solution conveying mechanism, and can provide required culture solution. The working process of the technical scheme is as follows: can take out culture dish body 5 in the incubator through transporting manipulator 2 to place culture dish body 5 on culture dish workstation 4, stretch into culture dish body 5 through liquid changing mechanism 3 with imbibition syringe needle 3.1.5 and carry out the imbibition, the back is accomplished to the imbibition, stretches into culture dish body 5 with liquid feeding needle tubing 3.1.2 and adds the culture solution, then sends culture dish body 5 back to the incubator through transporting manipulator 2. The whole liquid changing process in the technical scheme realizes full-automatic liquid changing, and reduces operation errors and pollution risks caused by manual operation.
Example 2:
as shown in fig. 1 and 3, on the basis of embodiment 1, a waste needle collecting box 7 is arranged on the fixing platform 1, a needle removing block 8 is fixed above the waste needle collecting box 7, and a plurality of unused liquid suction needles 3.1.5 are arranged in order. As shown in fig. 6, the needle releasing block 8 is provided with a limit groove 8.1 adapted to the liquid suction needle head 3.1.5, a positioning column 13 is arranged around the waste liquid discharging barrel 12, and the positioning column 13 is fixed with the fixing platform 1. The outer side wall of the liquid suction needle tube 3.1.3 is provided with a snap ring 3.1.4, and the liquid suction needle tube 3.1.3 is clamped with a liquid suction needle head 3.1.5 on the liquid suction needle tube 3.1.3 through the snap ring 3.1.4. Fixed platform 1 is last to be equipped with abandonment syringe needle and to collect box 7, and 7 tops of abandonment syringe needle are collected the box are fixed with and take off needle piece 8, take off and be equipped with the spacing groove 8.1 with imbibition syringe needle 3.1.5 adaptation on the needle piece 8. The upper end of the imbibition needle head 3.1.5 is provided with a limit ring 3.1.6, and the outer diameter of the limit ring 3.1.6 is larger than the width of the limit groove 8.1. A positioning column 13 is arranged around the waste liquid discharge barrel 12, and the positioning column 13 is fixed with the fixed platform 1.
Several unused pipette tips 3.1.5 are arranged in order. When a plurality of hole sites are arranged on the culture dish body 5, in order to avoid mutual pollution among the hole sites, after waste liquid in each hole site is absorbed, the liquid absorption needle head 3.1.5 needs to be replaced, and the needle head placing frame 6 is used for placing the unused liquid absorption needle head 3.1.5. The imbibition syringe needle 3.1.5 itself has certain elasticity, can block imbibition syringe needle 3.1.5 through snap ring 3.1.4. Technical scheme can be used for making imbibition syringe needle 3.1.5 break away from imbibition needle tubing 3.1.3, imbibition syringe needle 3.1.5 after inhaling the waste liquid, need change, move actuating mechanism 3.2 and remove imbibition syringe needle 3.1.5 to spacing groove 8.1 below, then upwards lifting syringe needle module 3.1, make and take off needle piece 8 and block the upper end of imbibition syringe needle 3.1.5, make imbibition syringe needle 3.1.5 break away from imbibition needle tubing 3.1.3, fall into in abandonment syringe needle collection box 7. The positioning column 13 can position and fix the waste needle collecting box 7.
Example 3:
as shown in fig. 5, on the basis of embodiment 1, the culture dish worktable 4 includes a fixed seat 4.1 and a rotating seat 4.2 which are arranged side by side, the fixed seat 4.1 is fixed with the fixed platform 1, the rotating seat 4.2 is rotatably connected with the fixed platform 1, a rotating shaft 4.3 of the rotating seat 4.2 is arranged in parallel with a horizontal plane, a rotating driver 4.5 for driving the rotating seat 4.2 to rotate is arranged on the fixed platform 1, and when the culture dish body 5 is placed on the rotating seat 4.2 during liquid changing. The rotating shaft 4.3 of the rotating seat 4.2 is eccentrically arranged below the rotating seat 4.2, and the rotating damper is arranged on the rotating shaft 4.3. The rotary driver 4.5 comprises an electromagnet, the electromagnet is fixed below the rotary seat 4.2, the rotary shaft 4.3 is arranged between the gravity center of the rotary seat 4.2 and the electromagnet, the magnetic attraction block 4.6 is arranged at the position of the lower end of the rotary seat 4.2 corresponding to the electromagnet, and when the electromagnet is electrified, the electromagnet is in contact with the magnetic attraction block 4.6. An adjusting base 10 is arranged below the rotating base 4.2, the adjusting base 10 is fixed with the fixed platform 1, an adjusting rod 11 which is in threaded connection with the adjusting base 10 and can be adjusted up and down is arranged on the adjusting base 10, the top end of the adjusting rod 11 is in contact with the bottom end of the rotating base 4.2, and the adjusting rod 11 and the electromagnet are arranged at two opposite ends of the rotating shaft 4.3.
Fixing base 4.1 is equipped with the holding tank 4.4 that is used for holding transport plate 2.4 of transporting manipulator 2. One end of the rotating seat 4.2, which is far away from the fixed seat 4.1, is provided with a limiting block 4.7 for limiting the position of the culture dish body 5. The lower end of the liquid adding needle tube 3.1.2 is provided with a liquid adding needle head 3.1.7, and the lower end of the liquid adding needle head 3.1.7 is bent towards the lower side of the rotating seat 4.2 after being inclined.
Among the above-mentioned technical scheme, after transfer manipulator 2 takes out culture dish body 5 in with the incubator, place on fixing base 4.1, then culture dish plectrum 9 can remove culture dish body 5 to rotating on seat 4.2, rethread rotates driver 4.5 and makes and rotate seat 4.2 and rotate the slope, make culture dish body 5 slope, the imbibition syringe needle 3.1.5 of being convenient for absorbs the waste liquid totally, then the liquid feeding is carried out to rethread liquid feeding needle tubing 3.1.2, the liquid feeding is accomplished the back, it makes and rotates seat 4.2 and rotates the level of returning to rotate 4.5 to rotate the driver, culture dish plectrum 9 can move culture dish body 5 to on the fixing base 4.1, can send culture dish body 5 back to the incubator through transferring manipulator 2. The structure can realize the rotation of the rotating seat 4.2, the rotating structure is simple and reliable, the cost is low, the control is simple, and the rotation can be realized only by one electromagnet. And the rotating seat 4.2 is kept horizontal by the gravity of the rotating seat 4.2.
The beneficial effects of the utility model are: (1) the whole liquid changing process realizes full-automatic liquid changing, and reduces operation errors and pollution risks caused by manual operation; (2) the suction needle head 3.1.5 can be automatically replaced; (3) the liquid outlet of the liquid adding needle 3.1.7 is close to the side wall of one side of the culture medium, so that the cell culture solution slowly flows down along the side surface of the culture medium, and the cells attached to the side wall and the bottom surface are prevented from being washed away by the liquid flow too fast; (4) the rotating seat 4.2 is rotated and inclined by rotating the driver 4.5, so that the culture dish body 5 is inclined, and the liquid absorption needle 3.1.5 can absorb the waste liquid completely; (5) the rotating structure of the rotating seat 4.2 is simple and reliable, the cost is low, the control is simple, the rotation can be realized only by one electromagnet, and the rotating seat 4.2 is kept horizontal by the gravity of the rotating seat 4.2.
Claims (10)
1. The utility model provides a full-automatic cell trades liquid device which characterized by includes:
a fixed platform;
the transfer manipulator is used for the culture dish body and is arranged on the fixed platform;
the liquid changing mechanism is arranged on the fixed platform and comprises a needle head module and a moving driving mechanism used for moving the needle head module, the needle head module comprises a fixed block, a liquid adding needle tube and a liquid absorbing needle tube, the liquid adding needle tube and the liquid absorbing needle tube are fixed on the fixed block, and the liquid absorbing needle tube is provided with a liquid absorbing needle;
The culture dish workbench is used for placing the culture dish body.
2. The apparatus of claim 1, wherein the movement driving mechanism comprises an X-axis moving mechanism, a Y-axis moving mechanism and a Z-axis moving mechanism, the needle module is fixed on the Z-axis moving mechanism, the Z-axis moving mechanism is fixed on the Y-axis moving mechanism, and the Y-axis moving mechanism is fixed on the X-axis moving mechanism.
3. The apparatus as claimed in claim 1, wherein the fixing platform is provided with a needle rack, and the needle rack is provided with a plurality of unused liquid-absorbing needles.
4. The automatic cell liquid changing device according to claim 3, wherein a positioning column is disposed around the needle placing rack, and the positioning column is fixed to the fixing platform.
5. The full-automatic cell liquid changing device as claimed in claim 1, 2, 3 or 4, wherein a snap ring is arranged on the outer side wall of the liquid suction needle tube, and the liquid suction needle tube is clamped with a liquid suction needle head on the liquid suction needle tube through the snap ring.
6. The full-automatic cell liquid changing device according to claim 1, 2, 3 or 4, wherein a culture dish shifting sheet is fixed on the fixing block and used for transversely shifting the culture dish body on the culture dish workbench.
7. The device according to claim 1, 2, 3 or 4, wherein the culture dish platform comprises a fixed base and a rotating base which are arranged side by side, the fixed base is fixed with the fixed platform, the rotating base is rotatably connected with the fixed platform, the rotating shaft of the rotating base is parallel to the horizontal plane, a rotating driver for driving the rotating base to rotate is arranged on the fixed platform, and the culture dish body is placed on the rotating base during liquid changing.
8. The fully automatic cell liquid changing device according to claim 1, 2, 3 or 4, wherein a waste liquid discharging barrel is arranged on the fixed platform.
9. The apparatus as claimed in claim 8, wherein the waste liquid discharge barrel is surrounded by a positioning post, and the positioning post is fixed to the fixing platform.
10. The full-automatic cell liquid changing device according to claim 1, 2, 3 or 4, wherein a microscope photographing station is arranged on the fixing platform, and a photographing fixing seat matched with the culture dish body is fixed on the microscope photographing station.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122907345.3U CN216838006U (en) | 2021-11-22 | 2021-11-22 | Full-automatic cell liquid changing device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122907345.3U CN216838006U (en) | 2021-11-22 | 2021-11-22 | Full-automatic cell liquid changing device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN216838006U true CN216838006U (en) | 2022-06-28 |
Family
ID=82104159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122907345.3U Active CN216838006U (en) | 2021-11-22 | 2021-11-22 | Full-automatic cell liquid changing device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN216838006U (en) |
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2021
- 2021-11-22 CN CN202122907345.3U patent/CN216838006U/en active Active
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