CN104673628A - Heat-preservation culturing device with gas control function - Google Patents
Heat-preservation culturing device with gas control function Download PDFInfo
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- CN104673628A CN104673628A CN201510083000.7A CN201510083000A CN104673628A CN 104673628 A CN104673628 A CN 104673628A CN 201510083000 A CN201510083000 A CN 201510083000A CN 104673628 A CN104673628 A CN 104673628A
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Abstract
The invention discloses a heat-preservation culturing device with a gas control function. The device comprises a hot vessel, wherein a top cover covers the hot vessel; a sealing space is formed between the top cover and the hot vessel, and the temperature in the space is guaranteed through the hot vessel; the gas can be introduced into the space to ensure that a specific gas environment in the sealing space can be obtained; a sample culture vessel is placed in the sealing space; meanwhile, the sealing space is so transparent as to be conveniently observed. The device is reasonable in design and simple and attractive in appearance; by using the device, the sample culture vessel can be integrally wrapped, so that the problem of the temperature loss in an operating process outside the box can be solved to the maximum limit, and the corresponding gas can also be introduced to build a culture environment of a gas culture box; compared with the size of a table culture box, the heat-preservation culturing device is relatively small in size, and a heating tabletop of an ultra-clean working station can be utilized to the maximum limit.
Description
Technical field
The present invention relates to a kind of IVF laboratory heat-preserving equipment, particularly relate to a kind of heat insulating culture device controlled with gas, be applicable to insulation and the cultivation of the long-time manipulation in vitro of sample.
Background technology
The multiple operation in current IVF laboratory all relates to the operation of sample for a long time outside incubator, sample to temperature and culture environment comparatively responsive, although all operations all carries out on warm platform, culture dish is except bottom contacts warm platform, and other directions all exist loses temperature phenomenon faster.Especially the manipulation require interval operation had, can only take by multiple switching incubator under existence conditions, and the process of taking all can produce adverse influence for other culture dish in single culture dish and incubator.Also have some small-sized desktop incubators on the market at present, but need to be put into inside Bechtop, and need Power supply to keep temperature, this not only occupies a large amount of desk-top spaces, and culture dish comparatively small amt deposited by little incubator.
Summary of the invention
In view of present situation, the present invention aims to provide a kind of heat insulating culture device controlled with gas, structure is simple, easy to operate, space availability ratio is high, suitable temperature and atmosphere surrounding can be provided, long-time case operates outward and culture sample, to solve the problem that existing IVF laboratory sample case operates and cultivates outward.
Object of the present invention is mainly achieved through the following technical solutions: a kind of heat insulating culture device controlled with gas, comprise a hot ware, buckle closure one top cover on described hot ware, form a space sealed between described top cover and hot ware, the temperature in this space is ensured by hot ware; Gas can be passed into make in seal cavity, to obtain specific atmosphere surrounding in this space; Place sample cultivation ware in this seal cavity, meanwhile, this seal cavity or transparently can to observe.
Further, described hot ware is from hot type, adopts lagging material to make; Described hot ware is hollow structural members, arranges electric heater unit in inside, by civil power or battery power, arranges switch and outlet in hot ware appearance; Be provided with an inlet mouth at the back side of described hot ware, inlet mouth is connected with inlet suction port, inlet suction port outer end connects external feed stream pipe, inner connection one airway; The top center position of self-heating ware is arranged to a platform sunk downwards, described sagging platform is offered the penetrating putting hole bottom a through hot ware, the zygomorphy of putting hole has hole of taking, for placing sample cultivation ware in putting hole, described sagging platform also offers a production well, and described airway is communicated with production well with described inlet mouth; Arrange a circle sealing-ring at described sagging flat edge of table, top cover lid is located on this platform, is sealed by sealing-ring.
Or described hot ware is passive hot type, the metallic substance of heat conduction is adopted to make; Described hot ware is entity structure part, is provided with an inlet mouth, inlet mouth is connected with inlet suction port at the back side of described hot ware, and inlet suction port outer end connects external feed stream pipe, is provided with a gas passage in hot ware inside; The top center position of self-heating ware is arranged to a platform sunk downwards, described sagging platform is offered the penetrating putting hole bottom a through hot ware, the zygomorphy of putting hole has hole of taking, for placing sample cultivation ware in putting hole, described sagging platform also offers a production well, and described gas passage is communicated with production well with described inlet mouth; Arrange a circle sealing-ring at described sagging flat edge of table, top cover lid is located on this platform, is sealed by sealing-ring.
Or described hot ware is passive hot type, the metallic substance of heat conduction is adopted to make; Described hot ware is entity structure part; Be provided with an inlet mouth at the back side of described hot ware, inlet mouth is connected with inlet suction port, inlet suction port outer end connects external feed stream pipe, is provided with a gas passage in hot ware inside; Revolve from the top of described hot ware and dig out the fluting that a circle has certain depth and width, described top cover is inserted in described fluting; Region within fluting sets out the penetrating putting hole bottom a through hot ware, and the zygomorphy of putting hole has hole of taking, and the region within fluting also offers a production well, and described gas passage is communicated with production well with described inlet mouth.
Further, a transparent sheet glass is placed in the bottom of described penetrating putting hole.
Further, described hot ware both sides external arranges two handles.
Further, described hot ware is rectangular parallelepiped, and length is 3-25cm, and width is 2-20cm, is highly 1-10cm.
Further, described metallic substance is one of metal materials such as copper, iron, stainless steel, aluminum magnesium alloy, aluminium, tin.
Further, described top cover is transparent plastics or glass material, and plastic material is one of polyethylene, polypropylene, polyvinyl chloride, polystyrene, ABS, polymethylmethacrylate, polymeric amide; Glass material is one of glass materials such as soda glass, potassic glass, magnalium glass, lead glass, borosilicate glass, silica glass.
Further, the shape of putting hole can be one of various ways such as circular, square, Polygons.
A kind of heat insulating culture device controlled with gas of the present invention, has following outstanding beneficial effect: 1, reasonable in design, succinctly attractive in appearance, small volume and less weight.2, sample culture dish entirety is wrapped in sample putting hole, solves the mistake temperature problem in the outer operating process of case to greatest extent.3, corresponding gas can be passed into, build the culture environment of gas incubator, the case of short period of time can place outward, cultivate.4, sample putting hole can adaptive existing all kinds of culture dish.5, heat insulating culture device is less relative to the size of desktop incubator, can utilize the heating table top of ultra-clean workstation to greatest extent, places multiple heat insulating culture device.
Other features and advantages of the present invention are set forth in explanation subsequently, and object of the present invention and other advantages realize by structure specifically noted in write specification sheets, claims and accompanying drawing and obtain.
Accompanying drawing explanation
Fig. 1 be a kind of with gas control the top cover of heat insulating culture device and the combination embodiment one of platform;
Fig. 2 is a kind of vertical view of the heat insulating culture device with gas control;
Fig. 3 be a kind of with gas control the top cover of heat insulating culture device and the combination embodiment two of platform;
Fig. 4 be a kind of with gas control the top cover of heat insulating culture device and the combination embodiment three of platform;
Fig. 5 is top cover is schematic diagram.
In figure, the hot ware of 1-, 2-inlet suction port, 3-air inlet switch, 4-external feed stream pipe, 5-airway, 5 '-gas passage, the platform that 6-sink, 7-top cover, 8-production well, 9-sample putting hole, 10-sample is taken hole, 11-sheet glass, 12-handle, 13-rubber seal, 14-slots, 15-handle.
Embodiment
Specifically describe the preferred embodiments of the present invention below in conjunction with accompanying drawing, wherein, accompanying drawing forms the application's part, and together with embodiments of the present invention for explaining technical scheme of the present invention.
As shown in Figure 1, a kind of heat insulating culture device controlled with gas of the present invention is suitable for the device that IVF laboratory sample case preserves and observe outward.
The general planning taked is: comprise a hot ware 1, this hot ware has heat insulation function; Buckle closure one top cover 7 on hot ware, can construct a space sealed between top cover 7 and hot ware 1.The temperature in this space can make temperature be protected by hot ware; Can gas be passed in this space, make to obtain specific atmosphere surrounding in seal cavity; Meanwhile, this seal cavity or transparently can to observe, because sample cultivation ware will be put into inside.Sample cultivation ware is put in this seal cavity, midway running can be taken out again, when doing Microscopic observation, together move on worktable together with hot ware, such device can to ensure in the observation in vitro of sample and the whole process of cultivation temperature, wet, compression ring border invariable, be conducive to the cultivation of active body.
Under above-mentioned general planning, we can have multiple feasible enforcement means.As, hot ware 1, profile can be the various ways such as square, rectangular parallelepiped, right cylinder; Be preferably rectangular parallelepiped, process like this, carry, placing articles is all more convenient.Further say, rectangular parallelepiped length is 3-25cm, and width is 2-20cm, is highly 1-10cm, and compact quality is light, can conveniently carry.
The heating of hot ware can adopt from heating or passive heat protocol, because the heat protocol taked is different, also can bring difference to the structure of hot ware inside.So come for two example two with different type of heating below.
When adopting from heat protocol, hot ware 1 should adopt lagging material to make.Hot ware is hollow, can arrange electrical heating wire or other any feasible heating units are all right in hot ware inside, and electric heater unit can by mains-supplied, external power supply socket, also by battery power, can arrange electric switch in hot ware side.Device heating belongs to mature technology, and these are all possible technique.
Further, can set temperature sensor, for monitoring temperature in ware.
Be provided with an inlet mouth in portion on the lower at the back side of hot ware 1, inlet mouth be connected with inlet suction port 2 (as shown in Figure 1), with hot ware compact siro spinning technology, inlet suction port 2 is provided with air inlet switch 3, control air input and induction air flow ratio.Inlet suction port 2 outer end connects external feed stream pipe 4, and it is inner that gas is passed into hot ware by inlet pipe and inlet suction port; Airway 5 is connected, by the place required for gas channeling, as shown in Figure 2 in inlet suction port 2 the inner.Corresponding premixed gas is can be accessed by by diffuser.Preferably, described inlet suction port 2 diameter elects 2-20mm as, and length is 5-50mm, and air input controls well.Described air inlet switch 3 is incorporated on inlet suction port 2, and in order to control flow and the flow velocity of gas, its size matches with inlet suction port.
The top center position of self-heating ware, is arranged to a platform 6 sunk downwards, situation as shown in Figure 1.Offer a penetrating putting hole 9 in the middle position of described sagging platform 6, the zygomorphy of putting hole has hole 10 of taking.For placing sample cultivation ware in putting hole 9, the hole of taking on both sides is convenient in order to pick and place planchet, and vertical view as shown in Figure 2.Described sagging platform 6 also offers a production well 8, and production well 8 is connected with airway 5, can from then on give vent to anger.Arrange a circle sealing-ring 13 at the edge of described sagging platform 6, top cover 7 lid is located on this platform, ensures stopping property by sealing-ring.Be arranged to the platform 6 sunk, its benefit one is that this concave manner can make podium level reduce, and sample cultivation ware can be made to sink to portion in hot ware as much as possible, absorb the heat of hot ware; Two is that platform sink and can increase sample and hold space, thus also can reduce the height of top cover or increase airtight space; Three is that this depressed platform can make top cover 7 can extend into below hot ware end face, and snap into better on the platform of depression, the top cover of buckle closure is more firm.
The shape of putting hole can be one of various ways such as circular, square, Polygons, and circular, diameter is 0.5-18cm.
When passive heat protocol selected by hot ware 1, be chosen as metal material, the good pure metal of preferred thermal conductivity, especially in the bottom of hot ware, should have good heat-conducting effect, being placed into by hot ware just can from bottom-heated on heating unit, vessel inside is heated, the portion in vessel that can not be used in like this arranges heating unit, by the temperature of the thermally conductive material or hot-plate that control hot ware, just can control temperature in ware.
Select the hot ware of metal material to be solid member, the heat conduction of the hot ware of such guarantee is fast.Be provided with an inlet mouth in portion on the lower at the back side of hot ware 1, inlet mouth be connected with inlet suction port 2 (as shown in Figure 1), with hot ware compact siro spinning technology, inlet suction port 2 is provided with air inlet switch 3, control air input and induction air flow ratio.Inlet suction port 2 outer end connects external feed stream pipe 4, and it is inner that gas is passed into hot ware by inlet pipe and inlet suction port.Be provided with a gas passage 5 ' in hot ware inside, gas passage 5 ' is all communicated with inlet end, outlet side, and gas passage can be reserved out when hot ware processing and fabricating or come outbreak out.
Top cover 7 and solid hot ware constructs the mode of enclosed space, can the top center position of self-heating ware, are arranged to a sagging platform 6 downwards, situation as shown in Figure 3.Offer a penetrating putting hole 9 in the middle position of described sagging platform 6, the zygomorphy of putting hole has hole 10 of taking.For placing sample cultivation ware in putting hole 9, the hole of taking on both sides is convenient in order to pick and place planchet, and vertical view as shown in Figure 2.Described sagging platform 6 also offers a production well 8, and production well 8 is communicated with gas passage 5 ', can from then on give vent to anger.Arrange a circle sealing-ring 13 at the edge of described sagging platform 6, top cover 7 lid is located on this platform, ensures stopping property by sealing-ring.Be arranged to getting well to be in and aforementionedly saying of the platform 6 sunk.
Because metal fever ware is solid member, do not have in other words so the transparence of observing is bad at all, so preferably place the sheet glass 11 of a high transparency in the bottom of penetrating putting hole 9, be beneficial to bottom surface daylighting to observe, the form of sheet glass can just like Fig. 3 and Fig. 4 two kinds, and glass can be fixed with caking agent.
Top cover 7 and solid hot ware constructs the mode of enclosed space, can also be that the top of self-heating ware is revolved and dug out the fluting 14 that a circle has certain depth and width, this slot in for inserting top cover 7.Region within fluting 14 sets out a penetrating putting hole 9, and the zygomorphy of putting hole has hole 10 of taking, and also has a production well 8.The effect in putting hole 9 and hole 10 of taking from the above.The width of fluting 14 is not easily too wide, just passable can put into top cover, should have certain depth.Because fluting is not only narrow but also dark, this mode places top cover, and the enclosed space constructed between hot ware should have more sealing effectiveness.
Similarly, also can place the sheet glass 11 of a high transparency in the bottom of penetrating putting hole 9, be beneficial to bottom surface daylighting and observe, the form of sheet glass can just like Fig. 3 and Fig. 4 two kinds, and glass can be fixed with caking agent.
The shape of the above penetrating putting hole 9 can be one of various ways such as circular, square, Polygons, and circular, diameter is 0.5-18cm.
Further, better at hot ware outer setting two handles 15, conveniently guide and support.
The above sample is taken hole 10, is positioned at sample putting hole both sides, symmetry arrangement, and can be one of shape such as square, circular, semicircle, preferably semicircle, radius is 0.5-5cm.
Above-described top cover 7, as shown in Figure 5, its shape, size and the hot ware of joining match.Can be rectangular parallelepiped, square or right cylinder accordingly, is transparent plastics or glass material, preferred glass material, and thickness of glass is 0.1-1cm; Preferred rectangular parallelepiped, its length is 2-24cm, and width is 1-19cm, is highly 1-10cm.Further, can arrange handle 12 at top cover 7 top, material is identical with top cover, and handle is positioned at the central top of top cover, and with top cover compact siro spinning technology, shape can be spheroid, square body or other forms, has had handle to be convenient to taking of top cover.
In the above embodiment, have portion of techniques feature to exchange or general, the present invention is not limited to above-mentioned exemplified several embodiments.
Material in full, metal material can be one of metal materials such as copper, iron, stainless steel, aluminum magnesium alloy, aluminium, tin, plastic material can be one of plastic materials such as polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), ABS, polymethylmethacrylate (PMMA), polymeric amide (PA), glass material can be one of glass materials such as soda glass, potassic glass, magnalium glass, lead glass, borosilicate glass, silica glass, quality of rubber materials can be natural rubber (NR), styrene-butadiene rubber(SBR) (SBR), cis-1,4-polybutadiene rubber (BR), polyisoprene rubber (IR), chloroprene rubber (CR), isoprene-isobutylene rubber (IIR), nitrile rubber (NBR), hydrogenation nitrile rubber (HNBR), ethylene-propylene rubber(EPR) (EPM EPDM), silicon rubber (Q), viton (FPM), urethanes (AU EU), acrylic elastomer (ACM AEM), chlorosulfonated polyethylene rubber (CSM), epichlorohydrin rubber (CO ECO), one of chlorinated polyethylene rubber (CM or CPE).
Can be found out by the selection of the selection of above-mentioned each parts, type selecting, size, this device is a small sample operating gear, light desirable.Use this device, both can have a temperature guarantee to sample, the guarantee of a seal cavity can also be had, a specific atmosphere surrounding can also be provided.
The use of this device is described with the embodiment of an operation below:
During the heat insulating culture device 1, using this band gas to control, first the hot ware building top cover is placed on the heating panel of ultra-clean workstation, (this kind is passive hot type to rely on heating panel to make its hot ware internal temperature reach desired temperature, if active heated type can without heating panel), this kind of device need not be placed into inside heating container, and operation is very easy.
2, the inlet pipe of premix gas is inserted into intake interface place, opens air inlet switch, make premix gas from production well out, be full of in the enclosed space of top cover and the construction of hot ware.
When 3, putting into sample cultivation in advance, first close gas, open top cover, the hand-held culture dish being placed with sample, to be taken hole by sample, culture dish is put into sample putting hole, and then build top cover, ventilation, can build the culture environment of gas incubator, the case that can realize the sample short period of time is placed outward, is cultivated.
4, during operation, hot ware and culture dish integral translation under mirror, air inlet switch is closed, and top cover is taken away, observe accordingly and operate, when operating like this, just the moment can keep desired temperature.
In sum; the embodiment of the present invention demonstrates a kind of practicable insulation gas cultivating operating apparatus; it will be appreciated by those skilled in the art that; above-described embodiment is only the present invention's preferably embodiment; but protection scope of the present invention is not limited thereto; anyly be familiar with those skilled in the art in the technical scope that the present invention discloses, the change that can expect easily or replacement, all should be encompassed within protection scope of the present invention.
Claims (10)
1., with the heat insulating culture device that gas controls, it is characterized in that: comprise a hot ware, buckle closure one top cover on described hot ware, form a space sealed between described top cover and hot ware, the temperature in this space is ensured by hot ware; Gas can be passed into make in seal cavity, to obtain specific atmosphere surrounding in this space; Place sample cultivation ware in this seal cavity, meanwhile, this seal cavity or transparently can to observe.
2. heat insulating culture device according to claim 1, is characterized in that: described hot ware is from hot type, adopts lagging material to make; Described hot ware is hollow structural members, arranges electric heater unit in inside, by civil power or battery power, arranges switch and outlet in hot ware appearance;
Be provided with an inlet mouth at the back side of described hot ware, inlet mouth is connected with inlet suction port, inlet suction port outer end connects external feed stream pipe, inner connection one airway;
The top center position of self-heating ware is arranged to a platform sunk downwards, and described sagging platform is offered the penetrating putting hole bottom a through hot ware, and the zygomorphy of putting hole has hole of taking, for placing sample cultivation ware in putting hole,
Described sagging platform also offers a production well, and described airway is communicated with production well with described inlet mouth;
Arrange a circle sealing-ring at described sagging flat edge of table, top cover lid is located on this platform, is sealed by sealing-ring.
3. heat insulating culture device according to claim 1, is characterized in that: described hot ware is passive hot type, adopts the metallic substance of heat conduction to make; Described hot ware is entity structure part;
Be provided with an inlet mouth at the back side of described hot ware, inlet mouth is connected with inlet suction port, inlet suction port outer end connects external feed stream pipe, is provided with a gas passage in hot ware inside;
The top center position of self-heating ware is arranged to a platform sunk downwards, and described sagging platform is offered the penetrating putting hole bottom a through hot ware, and the zygomorphy of putting hole has hole of taking, for placing sample cultivation ware in putting hole,
Described sagging platform also offers a production well, and described gas passage is communicated with production well with described inlet mouth;
Arrange a circle sealing-ring at described sagging flat edge of table, top cover lid is located on this platform, is sealed by sealing-ring.
4. heat insulating culture device according to claim 1, is characterized in that: described hot ware is passive hot type, adopts the metallic substance of heat conduction to make; Described hot ware is entity structure part;
Be provided with an inlet mouth at the back side of described hot ware, inlet mouth is connected with inlet suction port, inlet suction port outer end connects external feed stream pipe, is provided with a gas passage in hot ware inside;
Revolve from the top of described hot ware and dig out the fluting that a circle has certain depth and width, described top cover is inserted in described fluting;
Region within fluting sets out the penetrating putting hole bottom a through hot ware, and the zygomorphy of putting hole has hole of taking, and the region within fluting also offers a production well, and described gas passage is communicated with production well with described inlet mouth.
5. the heat insulating culture device according to Claims 2 or 3 or 4, is characterized in that: a transparent sheet glass is placed in the bottom of described penetrating putting hole.
6. according to the heat insulating culture device one of claim 1 to 5 Suo Shu, it is characterized in that: described hot ware both sides external arranges two handles.
7. according to the heat insulating culture device one of claim 1 to 6 Suo Shu, it is characterized in that: described hot ware is rectangular parallelepiped, length is 3-25cm, and width is 2-20cm, is highly 1-10cm.
8. the heat insulating culture device according to claim 3 or 4, is characterized in that: described metallic substance is one of metal materials such as copper, iron, stainless steel, aluminum magnesium alloy, aluminium, tin.
9. heat insulating culture device according to claim 1, is characterized in that: described top cover is transparent plastics or glass material, and plastic material is one of polyethylene, polypropylene, polyvinyl chloride, polystyrene, ABS, polymethylmethacrylate, polymeric amide; Glass material is one of glass materials such as soda glass, potassic glass, magnalium glass, lead glass, borosilicate glass, silica glass.
10. the heat insulating culture device according to Claims 2 or 3 or 4, is characterized in that: one of the shape of described putting hole is circular, square, polygonal in form.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510083000.7A CN104673628B (en) | 2015-02-16 | 2015-02-16 | The heat insulating culture device that a kind of band gas controls |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510083000.7A CN104673628B (en) | 2015-02-16 | 2015-02-16 | The heat insulating culture device that a kind of band gas controls |
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| CN104673628B CN104673628B (en) | 2016-12-07 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108467836A (en) * | 2018-05-07 | 2018-08-31 | 宁波华仪宁创智能科技有限公司 | Fertilized eggs culture apparatus and its working method |
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