CN2505484Y

CN2505484Y - High-efficiency microorganism cultivation device

Info

Publication number: CN2505484Y
Application number: CN 01247526
Authority: CN
Inventors: 邹渝
Original assignee: Individual
Current assignee: Individual
Priority date: 2001-09-06
Filing date: 2001-09-06
Publication date: 2002-08-14
Anticipated expiration: 2011-09-06

Abstract

The utility model discloses a high-efficiency microorganism cultivation device, wherein, a special gas generating cup is fixed inside a gas integration cover. The utility model has the advantages of easy and convenient operation, sufficient utilization of gas propellant, quick and pure formation of special gas environmental conditions, stable and reliable device, high cultivation success rate, random number of microorganism specimen cultivated at a time, etc.

Description

The high-effective microorganism culture apparatus

The utility model relates to a kind of microorganism culturing, and is mainly used in the device of anaerobic bacteria culture.

The inoculation of microorganism, cultivation, evaluation etc. often need be carried out in the culture environment of a certain specific gas.Present microbial cultivation device has: specific gas glove box, specific gas jar etc.Glove box costs an arm and a leg, and still need during use special exogenous specific gas and equipment are difficult to promote the wider application.The specific gas jar need to use special catalyst in use, and the ventilation of need bleeding when at every turn using, troublesome poeration, and only can be for the microorganism culturing use not too high to the requirement of specific gas envrionment conditions.Application number is 87205065 Chinese patent " liquid-seal type carbonic acid gas incubator ", is fit to require the cultivation of the anerobe of anaerobic or utmost point low oxygen concentration environment.It is easy to make, cost is low, but has following shortcoming: 1, can only place substratum and CO in the bottom ₂Propellant, once educable anerobe sample is few, and efficient is low; 2, carry out CO with hand ₂Sequence of operations such as propellant water mixing, placement culture dish, anaerobic gas produces in a large number, but this moment, whole incubator interior or exterior space fail to seal, isolate, anaerobic gas runs off in a large number, both formed waste, also be difficult for reaching the anaerobic environment requirement of regulation, cause the poor even failure of culture effect; 3, airtight initial stage gas production rate is big, and the device internal gas pressure increases severely, change, and culture dish easily produces displacement, bump, damages in device, and perhaps high atmospheric pressure is washed the transparent cover of going up open, and anaerobic environment is destroyed and fails.

It is light, simple and convenient that the purpose of this utility model just provides a kind of operation, the gas-evolution agent utilization is not fully, waste, the specific gas environment forms rapid, pure, good leak tightness, reliable and stable, the culture success ratio height, the new and effective microbial cultivation device that once educable microorganism sample is the amount doesn't matter.

The purpose of this utility model is to realize like this.Integrate cover by gas, lid gas displacement overhead guard thereon, a kind of high-effective microorganism culture apparatus that the vapor pipe on the overhead guard etc. constitute, its constructional feature is: gas is integrated the cover internal fixing has specific gas that cup takes place.

Above-mentioned gas is integrated in the cover and has been placed culturing rack vertically, is horizontally arranged with plural culture dish shelf on the culturing rack, and specific gas cup takes place is positioned in the middle of the culturing rack bottom or near the centre.

Have the slotted eye that inserts the different gas absorption bar of delaying type on the culture dish shelf on the above-mentioned culturing rack.

The top of above-mentioned overhead guard is the lonely shape of arch.

The vapor pipe of above-mentioned overhead guard is arranged on the inwall of overhead guard, and the inlet mouth on vapor pipe top, the venting port of bottom all are the flared horn shape.

Above-mentioned integration cover loam cake one is coated at the ring cowling of overhead guard, and the outer rim of this ring cowling is provided with downwards, the catching groove of indent, and the lug, nib of this catching groove and integration cover top outer rim matches.

The above-mentioned overhead guard position on the lower side of collar shroud outside is provided with rubber band.

Above-mentioned overhead guard bottom is provided with plural liquid stream exchange hole.

Below in conjunction with embodiment and accompanying drawing this practical full mold is done to describe in further detail.

Fig. 1 is the utility model embodiment cross-sectional view.

Fig. 2 is the perspective view of the utility model embodiment culturing rack and culture dish shelf thereof.

Fig. 1 illustrates, and a kind of embodiment of the present utility model is: integrate cover 1 by gas, and lid gas displacement overhead guard 2 thereon, in the high-effective microorganism culture apparatus that vapor pipe 3 grades on the overhead guard 2 constitute, gas is integrated cover 1 internal fixing has specific gas that cup 4 takes place.The concrete fixed form that cup 4 takes place can be to integrate cover 1 and cup 4 takes place to form incorporate one-piece construction.

Fig. 1, Fig. 2 illustrate, and gas is integrated in the cover 1 and placed culturing rack 5 vertically, is horizontally arranged with plural culture dish shelf 6 on the culturing rack 5, and specific gas cup 4 takes place is positioned in the middle of culturing rack 5 bottoms or near the centre.Have the slotted eye 8 that inserts the different gas absorption bar 7 of delaying type on the culture dish shelf 6 on the culturing rack 5.The quantity of culture dish shelf 6 can be for more than two, below ten during enforcement.Size, specification according to the utility model culture apparatus are different and different.

Fig. 1 also illustrates, and overhead guard 2 tops of the utility model embodiment are the lonely shape of arch.Vapor pipe 3 on the overhead guard 2 is arranged on the inwall of overhead guard 2, and the inlet mouth 9 on vapor pipe 3 tops, the venting port 10 of bottom all are bell mouth shape.

Integrate the ring cowling 11 that cover 1 loam cake one is coated at overhead guard 2, the outer rim of this ring cowling 11 is provided with downwards, the catching groove 12 of indent, and this catching groove 12 matches with the lug, nib 13 of integrating cover 1 top outer rim.Overhead guard 2 position on the lower side of collar shroud 11 outside is provided with rubber band 14.

Overhead guard (2) bottom is provided with plural liquid stream exchange hole (15).

Cultivation with anerobe is an example below, and operating process of the present utility model and working mechanism are described:

Operating process: specific gas is instead given birth to agent 16, is that this routine anaerobic gas propellant 16 is inserted in the specific gas generation cup 4, and with the different gas absorption bar 7 of delaying type, be that this routine delaying type oxygen absorption bar 7 inserts in slotted eyes 8, place on the culture dish shelf 6 culture dish 18 of inoculating anerobe sample training oxygen base 19 is housed.Again whole culturing rack 5 is placed on the generation cup 4 in the gas integration cover 1, covers overhead guard 2 and fastening ring cowling 11, integrate in the cover 1 with sealing liquid body 20, will install the internal and external environment partition toward gas by the liquid filling hole on the ring cowling 11 17 then.Liquid volume added can enter with liquid and take place in the cup 4, and satisfies the needs of anaerobism propellant 16 reactions and decide.Subsequently whole culture apparatus is put into thermostat container, behind the constant temperature culture specified time, get final product observations, complete operation.

Working mechanism: after the anaerobic gas propellant 16 immersion sealing fluids 20 in the cup 4 take place, produce reaction and generate a large amount of anaerobic gas, because the shelves that support of bottom shelf 6 at first flow downward anaerobic gas, the upwards extruding of aerobic air of cover bottom will be integrated, simultaneously because anaerobic gas proportion is bigger than aerobic air, make the interior aerobic air of device upwards flow rapidly, be guided to the inlet mouth 8 of bell mouth shape by the arc top of overhead guard 2, enter the pipeline of vapor pipe 3, venting port 9 through bell mouth shape, discharge outside the overhead guard 2, pass sealing fluid 20 with bubble form again, arrange to device outside through the liquid filling hole 17 of ring cowling 11 and other air outlets of ring cowling 11.Aerobic gas displacement in will installing is rapidly fallen, and forms anaerobic environment in device.

Delaying type oxygen absorption bar 7 bottoms are with after sealing fluid contacts, absorbing liquid slowly soaks into to its top, when the displacement exhaust process of guaranteeing anaerobic gas displacement aerobic air has been finished, the oxygen absorbent side on 7 tops of delaying type oxygen absorption bar absorbs liquid, oxygen absorbent is just had an effect, sponge with a small amount of surplus oxygen that will not be displaced in will installing, make the anaerobic environment in the device thorough, pure.

As required, the utility model adopts different specific gas propellants and the corresponding different gas absorption bar of delaying type thereof, promptly can be used for the cultivation of the microorganism of other specific gas environment (as oxygen environment) of requirement, and its operating process and working mechanism are similar.

Compared with prior art, the utlity model has following obvious advantage:

1, operation is easy and convenient, the saving gas propellant. Adding liquid operation, specific gas propellant Before the beginning aerogenesis, whole device cuts off with the external world, sealing, and all operations need not to worry anaerobic gas Run off, operation without any confusion, and is light, simple, convenient; The utilization of gas propellant fully, fully, Efficiently. Avoided prior art at a large amount of aerogenesis of gas propellant, also need carry out can after the series operation Housing, airtight, the waste of gas propellant is very big, the specific gas environment is difficult for reaching regulation concentration and wants and make Ask, also rapidly nervous because of operation requirements, easily produce maloperation, make the problems such as experiment failure.

2, device is stable, reliable, durable, the culture success ratio height, and it is the amount doesn't matter to cultivate number of samples. Specific Cup takes place and is fixed on the integration cover in gas, when having avoided activity to place, and air pressure change in the device, liquid stream Moving, the cup displacement takes place, rock, inflow is uncontrollable in the cup, makes the experiment failure, even produces bump, It is broken that cup takes place; Upper nearly overhead guard, lower supporting are integrated culturing rack and the culture dish shelf thereof that covers, so that culture medium Position stability in device is not shifted, and the assurance culture experiment is reliably carried out. Each shelf can be placed cultivation Base also can be vacant, and therefore, each microorganism sample of cultivating is the amount doesn't matter, very convenient; The ring lid, The non-rigid cooperation of rubber band, air pressure drastic change when effectively having alleviated a large amount of aerogenesis, device may produce Vibrations avoid device to damage, and make durable, the long service life of this device.

3, the specific gas environment forms rapidly, and is thorough, pure. The arc top of overhead guard, blast pipe Bell mouth shape gas outlet, exhaust outlet are conducive to the gas in the rapid displacement apparatus, and formation requires fast The specific gas environment avoids bacterium colony to be exposed to for a long time in the nonconforming gas environment, cultivates failure. Delaying type absorbs bar absorption just takes place after displacement is finished, and both has been conducive to the abundant profit of GAS ABSORPTION agent With, also make the specific gas environment more thorough, pure, can be used for specific gas environment condition is required very The cultivation of strict microorganism. The current exchange hole tends to balance rapidly the device external and internal pressure, in preventing External differential is excessive, suppresses the abundant reaction of gas propellant.

4, fully effectively utilizing of gas propellant, different GAS ABSORPTION agent, device reliable and stable, Durable, extremely high culture success ratio, the cultivation fee that effectively reduces microorganism is used; This device is made in addition Convenient, price is low, all is conducive to popularization of the present utility model, application, impels grass-roots unit's Popularization The gas environment there are the work such as cultivation, detection of the microorganisms such as anaerobism bacterium of special requirement, have well Economic benefit and social benefit.

Claims

1, a kind of high-effective microorganism culture apparatus is integrated cover (1) by gas, lid displacement overhead guard (2) thereon, and the vapor pipe formations such as (3) on the overhead guard (2) is characterized in that: gas is integrated cover (1) internal fixing has specific gas that cup (4) takes place.

2, a kind of high-effective microorganism culture apparatus according to claim 1, it is characterized in that: described gas is integrated in the cover (1) and has been placed culturing rack (5) vertically, be horizontally arranged with plural culture dish shelf (6) on the culturing rack (5), cup (4) takes place and is positioned near centre, culturing rack (5) bottom or the centre in specific gas.

3, a kind of high-effective microorganism culture apparatus according to claim 2 is characterized in that: have the slotted eye (8) that inserts the different gas absorption bar of delaying type (7) on the culture dish shelf (6) on the described culturing rack (5).

4, a kind of high-effective microorganism culture apparatus according to claim 1 is characterized in that: the top of described overhead guard (2) is the lonely shape of arch.

5, a kind of high-effective microorganism culture apparatus according to claim 1, it is characterized in that: the vapor pipe (3) on the described overhead (2) is arranged on the inwall of overhead guard (2), the inlet mouth (9) on vapor pipe (3) top, the venting port of bottom (10) all is bell mouth shape.

6, a kind of high-effective microorganism culture apparatus according to claim 1, it is characterized in that: described integration cover (1) loam cake one is coated at the ring cowling (11) of overhead guard (2), the outer rim of this ring cowling (11) is provided with downwards, the catching groove of indent (12), this catching groove (12) and (13) cooperation mutually of the lug, nib of integrating cover (1) top outer rim.

7, a kind of high-effective microorganism culture apparatus according to claim 6 is characterized in that: described overhead guard (2) position on the lower side of collar shroud (11) outside is provided with rubber band (14).

8, a kind of high-effective microorganism culture apparatus according to claim 1 is characterized in that described overhead guard (2) bottom is provided with plural liquid stream exchange hole (15).