CN2146491Y - Once-used anaerobes petri-type dish - Google Patents

Once-used anaerobes petri-type dish Download PDF

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Publication number
CN2146491Y
CN2146491Y CN 92231615 CN92231615U CN2146491Y CN 2146491 Y CN2146491 Y CN 2146491Y CN 92231615 CN92231615 CN 92231615 CN 92231615 U CN92231615 U CN 92231615U CN 2146491 Y CN2146491 Y CN 2146491Y
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petri
anaerobes
dish
once
typed
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Expired - Fee Related
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CN 92231615
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Chinese (zh)
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李建秋
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/28—Constructional details, e.g. recesses, hinges disposable or single use
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/46—Means for fastening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/50—Means for positioning or orientating the apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/06—Magnetic means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The utility model relates to a once-used anaerobes petri-typed dish, the problem that the fussy operation and high cost of the similar anaerobes apparatus (such as a glove box, an anaerobic tank, etc.) when the anaerobe cultured can be solved; the once-used anaerobes petri-typed dish is characterized in that the petri-typed dish is provided with a metal rod which can be rotated under the action of magnetic field, and the petri-typed dish is also provided with a rubber plug-hole used for closing through a rubber plug. The once-used anaerobes petri-typed dish has the advantages of simple structure, having reliable effect, and having low cost; the once-used anaerobes petri-typed dish can not be restricted to the operating environment.

Description

Once-used anaerobes petri-type dish
The utility model relates to microorganism (bacterium) culture apparatus, especially a kind of disposable anaerobic bacteria culture device, i.e. Disposable culture dish for anaerobe.
At present, the anaerobic bacteria culture device has both at home and abroad: anaerobism glove box, anaerobic jar etc.The anaerobism glove box costs an arm and a leg, and the special gas of still needing during use is difficult to promote the wider application.Anaerobic jar need to use " 825 " catalyzer or " Gas Pak " catalyzer in use, and each use ventilation of need bleeding, and it only can be for the use of cultivation moderate anerobe.
The utility model purpose is: at the problems referred to above design a kind of simple in structure, cost is low, the Disposable culture dish for anaerobe of easy handling.
Technical solution of the present utility model a: metal bar that rotates under the action of a magnetic field 4 is housed in culture dish, and on culture dish lid 1, also is provided with a rubber plug-hole 6 with stifled 3 sealings of rubber.
The utility model compared with prior art
A, the trouble that can remove existing preparation substratum from are convenient to clinical samples or are increased bacterium culture direct inoculation, and ready access upon use is simple to operate.
B, moderate anaerobic gas bacterium can not only be cultivated, and extreme anerobe can be cultivated again.
C, operation are under aerobic conditions cultivated and are checked Clinical Anaerobic Bacteria.
D, the built-in metal bar of this kind flat board are coated with the bacterium operation under the magnetic stirring apparatus effect, colony growth is even, and the single bacterium colony that is easy to grow is convenient to obtain pure growth.
E, with low cost, its specification and standard are unified, are convenient to commercialization and widespread use.
Accompanying drawing 1 is surface structure synoptic diagram of the present invention.
Accompanying drawing 2 is axle diagrammatic cross-section of the present invention.
In Fig. 1-2: at the bottom of 1 ware lid, 2 wares, stifled, 4 metal bars of 3 rubbers, 5 indicator grooves, 6 rubber plug-holes, 7,9 pressure sensitive membranes, the stifled breach of 8 rubbers.In this implementation column, the culture dish inner side-wall is provided with: one with 2 vertical indicator grooves 5 at the bottom of the ware, cover 1 bear building-up and close part and be fastening structure, the appearance pressure sensitive membrane 9 that rounds.At ware lid 1, and the top that is positioned at rubber stifled 3 is sealed with pressure sensitive membrane 7, this culture dish for anaerobe is made up of hard, transparent plastics and fittings thereof, the high 20mm of ware, the diameter 84mm of ware, 2 two portions at the bottom of branch ware lid 1 and the ware, lid, bear building-up close part and are fastening structure, the rubber plug-hole 6 that one diameter 19mm size is arranged apart from ware lid 20mm place, edge, the sticking pressure sensitive membrane 7 in top that rubber is stifled, there is the breach 8 of fixing metal rod stifled below, and there is the indicator groove 5 that the riser of a block length 35mm, high 12mm forms in 2 limit portions at the bottom of the ware.
The utility model using method and anaerobic bacteria culture process are as follows:
1, the method for making of disposable anaerobic bacteria culture flat board
Disposable culture dish for anaerobe sprinkled under oxygen free condition make flat board, use cobalt after the sealing 60Illumination-based disinfection is standby.
2, anaerobic bacteria culture method
Get postradiation disposable anaerobic bacteria culture flat board, uncover pressure sensitive membrane 7, extract the sealed rubber stifled 3 that punctures and pass through plate behind the bacterium liquid with asepsis injector or sample injector, in ware, add one of bacterium liquid, then syringe needle is tilted slightly just to touch metal bar 4 falling into media surface, be placed on plate on the magnetic stirring apparatus again and connect power supply, this moment is in the media surface metal bar rotation of this Disposable culture dish for anaerobe, with the coating of bacterium liquid evenly, put 37 ℃ of common incubators then and get final product.

Claims (2)

1, a kind of Disposable culture dish for anaerobe that comprises culture dish, its feature is as follows: a metal bar that rotates under the action of a magnetic field 4 is housed in culture dish, and also is provided with a rubber plug-hole 6 with stifled 3 sealings of rubber on culture dish lid 1.
2, Disposable culture dish for anaerobe according to claim 1 is characterized in that in the setting of culture dish inner side-wall:
A, one and 2 vertical indicator grooves 5 at the bottom of the ware;
B, lid 1 ends 2 bound fraction are fastening structure, the appearance pressure sensitive membrane 9 that rounds.
CN 92231615 1992-08-25 1992-08-25 Once-used anaerobes petri-type dish Expired - Fee Related CN2146491Y (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 92231615 CN2146491Y (en) 1992-08-25 1992-08-25 Once-used anaerobes petri-type dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 92231615 CN2146491Y (en) 1992-08-25 1992-08-25 Once-used anaerobes petri-type dish

Publications (1)

Publication Number Publication Date
CN2146491Y true CN2146491Y (en) 1993-11-17

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CN 92231615 Expired - Fee Related CN2146491Y (en) 1992-08-25 1992-08-25 Once-used anaerobes petri-type dish

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CN (1) CN2146491Y (en)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103173392A (en) * 2013-03-19 2013-06-26 云南省烟草农业科学研究院 Method for magnetically and rotatably applying bacteria on culture medium panel
CN103764812A (en) * 2011-08-30 2014-04-30 生物梅里埃公司 Methods for inoculating culture media on petri dishes by means of vibration frequencies
CN104928180A (en) * 2015-06-29 2015-09-23 重庆大学 Culture dish device based on base of micro-column type topological structure and application method of culture dish device in target responsiveness reinforcement
CN107216997A (en) * 2017-07-24 2017-09-29 东北林业大学 Bacterium solution apparatus for coating and coating method
CN113699029A (en) * 2021-07-21 2021-11-26 四川若斌生物科技有限责任公司 Automatic plate coating instrument and automatic plate coating method

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103764812A (en) * 2011-08-30 2014-04-30 生物梅里埃公司 Methods for inoculating culture media on petri dishes by means of vibration frequencies
CN103764812B (en) * 2011-08-30 2016-07-06 生物梅里埃公司 By the method that the culture medium on Petri dish is inoculated by frequency of vibration
CN103173392A (en) * 2013-03-19 2013-06-26 云南省烟草农业科学研究院 Method for magnetically and rotatably applying bacteria on culture medium panel
CN104928180A (en) * 2015-06-29 2015-09-23 重庆大学 Culture dish device based on base of micro-column type topological structure and application method of culture dish device in target responsiveness reinforcement
CN107216997A (en) * 2017-07-24 2017-09-29 东北林业大学 Bacterium solution apparatus for coating and coating method
CN113699029A (en) * 2021-07-21 2021-11-26 四川若斌生物科技有限责任公司 Automatic plate coating instrument and automatic plate coating method

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