CN103173392A - Method for magnetically and rotatably applying bacteria on culture medium panel - Google Patents
Method for magnetically and rotatably applying bacteria on culture medium panel Download PDFInfo
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- CN103173392A CN103173392A CN2013100877697A CN201310087769A CN103173392A CN 103173392 A CN103173392 A CN 103173392A CN 2013100877697 A CN2013100877697 A CN 2013100877697A CN 201310087769 A CN201310087769 A CN 201310087769A CN 103173392 A CN103173392 A CN 103173392A
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- magnetic stirring
- culture medium
- flat plate
- stirring bar
- medium flat
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Abstract
The invention discloses a method for magnetically and rotatably applying bacteria on a culture medium panel. The method comprises the steps of: heating, sterilizing, drying and cooling a magnetic stirring rod to the room temperature for standby application, wherein the length of the magnetic stirring rod is less than the internal diameter of the culture medium panel; arranging the magnetic stirring rod on a central axis of the culture medium panel, dripping a bacterium suspension liquid onto a culture medium through aiming at a central point position of the magnetic stirring rod, and then, covering a petri dish cover; putting the culture medium panel on a magnetic stirring apparatus, driving the magnetic stirring rod to rotate on the surface of the culture medium panel through the magnetic stirring apparatus, and uniformly applying the bacterium suspension liquid on the surface of the culture medium panel; and after the applying is finished, taking the magnetic stirring rod out, and sterilizing and cleaning the magnetic stirring rod for being repeated use. According to the method, the magnetic stirring rod serves as an application tool, and the bacterium suspension liquid is uniformly applied on the surface of the culture medium panel through utilizing the horizontal rotation of the magnetic stirring rod. The non-contact drive of the magnetic stirring rod is ingeniously utilized to apply bacteria in a closed state, so that the bacteria application efficiency is increased, and the operation errors of operators can be reduced.
Description
Technical field
The invention belongs to the Experiment on Microbiology technical field, be specifically related to a kind of method of culture medium flat plate magnetic vortex type coating bacterium.
Background technology
Culture medium flat plate coating bacterium is method commonly used in Experiment on Microbiology, is widely used in the operations such as bacterium separation, bacterial count, drug sensitive test, antagonism detection.Bacterium is adopted the spreading rod hardened coating usually, although easy, but this mode easily scratches media surface, more difficult coating is even, the problems such as personal errors also easily appear, and spreading rod operational requirement flat board must be in and open wide or half opening-wide state, and substratum and air are long duration of contact, are easy to cause living contaminants.For this reason, bacterium coating inoculation instrument occurred, this instrument is comprised of rotatable platform (manually with automatic two kinds) and the aseptic spreader (stainless steel spreading rod) of culture dish holding, can realize bacterium coating standardized work.The mode of operation of bacterium coating inoculation instrument is that culture dish is placed on universal stage, put appropriate sample liquid at the culture dish center, then rotation platform drives the culture dish rotation, sample liquid is got rid of to surrounding, then coordinate the platform rotation produce smooth and cultivate uniformly liquid film with stainless steel spreading rod or the aseptic spreading rod of disposable plastic.Although the Painting effect of this device is fine, still need culture medium flat plate is opened wide and could operate.For this reason, developing a kind of method that can realize the automation bacteria coating under closed state, is very favorable for Experiment on Microbiology.
Summary of the invention
The object of the present invention is to provide a kind of can be in the method for the culture medium flat plate magnetic vortex type coating bacterium of automatization coating bacterium under closed state.
The object of the present invention is achieved like this, comprises the following steps:
A, magnetic stirring bar sterilization: with dry after the magnetic stirring bar heat sterilization, be cooled to room temperature standby; The length of described magnetic stirring bar is less than the internal diameter of culture medium flat plate;
B, magnetic stirring bar setting: magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid;
The coating of C, bacterium: culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar and rotates on the culture medium flat plate surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
The present invention as spreading implement, utilizes the surface that bacterial suspension is uniformly coated on culture medium flat plate that horizontally rotates of magnetic stirring bar with magnetic stirring bar.The ingenious magnetic stirring bar non_contact drive that utilized of the present invention is realized bacterium coating under closed state; The steady fast rotational of magnetic stirring bar is coated with bacterial suspension fast, has improved coating efficiency; Magnetic stirring bar can concentrate sterilization to deposit, and has shortened the time that the coating apparatus is prepared; Utilize magnetic stirring bar coating bacterium can realize being coated with stdn, reduced the manual operation error, can obtain normalized coated sample at every turn.
Embodiment
The present invention is further illustrated below in conjunction with embodiment, but never in any form the present invention is limited, and any replacement that is equal to based on technical thought of the present invention all falls into protection scope of the present invention.
The present invention includes following steps:
A, magnetic stirring bar sterilization: with dry after the magnetic stirring bar heat sterilization, be cooled to room temperature standby; The length of described magnetic stirring bar is less than the internal diameter of culture medium flat plate;
B, magnetic stirring bar setting: magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid;
The coating of C, bacterium: culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar and rotates on the culture medium flat plate surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
Described magnetic stirring bar is the magnetic stirring bar of stainless steel, Armco magnetic iron or common iron, and magnetic stirring bar is the right cylinder of diameter 3 ~ 6mm.Described Armco magnetic iron or common ferromagnetic power stirring rod outer cladding one deck heat-resistant anticorrosive corrosion material.Described heat-resistant anticorrosive corrosion material is glass, pottery or polytetrafluoroethylmaterial material.
The described heat sterilization of A step is that magnetic stirring bar is contained in heat sterilization in thermally resistant container.
The described heat sterilization of A step is wet-heating or dry heating method sterilization.
The described magnetic stirring bar of C step rotates as clockwise rotating, rotate counterclockwise or suitable, counterclockwise alternate rotation.The described magnetic stirring bar rotation time of C step is 1 ~ 2min.
Plastics or glass culture dish that described culture medium flat plate uses, the culture dish diameter is 60mm, 90mm, 100mm or 120mm.
Principle of work of the present invention:
The present invention as spreading implement, utilizes the surface that bacterial suspension is uniformly coated on culture medium flat plate that horizontally rotates of magnetic stirring bar with magnetic stirring bar.The ingenious magnetic stirring bar non_contact drive that utilized of the present invention is realized bacterium coating under closed state; The steady fast rotational of magnetic stirring bar is coated with bacterial suspension fast, has improved coating efficiency; Magnetic stirring bar can concentrate sterilization to deposit, and has shortened the time that the coating apparatus is prepared; Utilize magnetic stirring bar coating bacterium can realize being coated with the stdn effect, reduced the manual operation error, can obtain normalized coated sample at every turn.
Embodiment 1
Select the culture medium flat plate of 60mm, the stainless steel magnetic stirring bar is the right cylinder of diameter 3mm, and length is less than the internal diameter of culture medium flat plate; Magnetic stirring bar is contained in thermally resistant container, and after the wet-heating sterilization, drying is cooled to room temperature standby; Magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid; Culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar and clockwise rotates 1min on the culture medium flat plate surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
Embodiment 2
Select the culture medium flat plate of 90mm, the Armco magnetic iron magnetic stirring bar that coats tetrafluoroethylene is the right cylinder of diameter 4mm, and length is less than the internal diameter of culture medium flat plate; Magnetic stirring bar is contained in thermally resistant container, and after the dry heating method sterilization, drying is cooled to room temperature standby; Magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid; Culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar and rotates counterclockwise 2min on the culture medium flat plate surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
Embodiment 3
Select the culture medium flat plate of 100mm, the right cylinder that the common ferromagnetic power stirring rod that coats tetrafluoroethylene is diameter 6mm, length is less than the internal diameter of culture medium flat plate; Magnetic stirring bar is contained in thermally resistant container, and after the wet-heating sterilization, drying is cooled to room temperature standby; Magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid; Culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar at culture medium flat plate suitable, the counterclockwise alternate rotation 2min in surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
Embodiment 4
Select the culture medium flat plate of 120mm, the right cylinder that the common ferromagnetic power stirring rod that coats glass is diameter 5mm, length is less than the internal diameter of culture medium flat plate; Magnetic stirring bar is contained in thermally resistant container, and after the dry heating method sterilization, drying is cooled to room temperature standby; Magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid; Culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar and rotates counterclockwise 1.5min on the culture medium flat plate surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
Embodiment 5
Select the culture medium flat plate of 90mm, the Armco magnetic iron magnetic stirring bar of coating ceramic is the right cylinder of diameter 4mm, and length is less than the internal diameter of culture medium flat plate; Magnetic stirring bar is contained in thermally resistant container, and after the wet-heating sterilization, drying is cooled to room temperature standby; Magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid; Culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar at culture medium flat plate suitable, the counterclockwise alternate rotation 1.5min in surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
Claims (9)
1. the method for culture medium flat plate magnetic vortex type coating bacterium is characterized in that comprising the following steps:
A, magnetic stirring bar sterilization: with dry after the magnetic stirring bar heat sterilization, be cooled to room temperature standby; The length of described magnetic stirring bar is less than the internal diameter of culture medium flat plate;
B, magnetic stirring bar setting: magnetic stirring bar is arranged on the central axis of culture medium flat plate, corresponding magnetic stirring bar center position drips bacterial suspension on substratum, then builds the culture dish lid;
The coating of C, bacterium: culture medium flat plate is placed on magnetic stirring apparatus, and magnetic stirring apparatus drives magnetic stirring bar and rotates on the culture medium flat plate surface, and bacterial suspension is evenly coated the culture medium flat plate surface; After coating is completed, take out the magnetic stirring bar sterilization and clean the reserve recycling.
2. culture medium flat plate magnetic vortex type according to claim 1 is coated with the method for bacterium, and it is characterized in that: described magnetic stirring bar is the magnetic stirring bar of stainless steel, Armco magnetic iron or common iron, and magnetic stirring bar is the right cylinder of diameter 3 ~ 6mm.
3. the method for culture medium flat plate magnetic vortex type coating bacterium according to claim 2, is characterized in that: described Armco magnetic iron or common ferromagnetic power stirring rod outer cladding one deck heat-resistant anticorrosive corrosion material.
4. culture medium flat plate magnetic vortex type according to claim 3 is coated with the method for bacterium, and it is characterized in that: described heat-resistant anticorrosive corrosion material is glass, pottery or polytetrafluoroethylmaterial material.
5. culture medium flat plate magnetic vortex type according to claim 1 is coated with the method for bacterium, and it is characterized in that: the described heat sterilization of A step is that magnetic stirring bar is contained in heat sterilization in thermally resistant container.
6. culture medium flat plate magnetic vortex type according to claim 1 is coated with the method for bacterium, it is characterized in that: the described heat sterilization of A step is wet-heating or dry heating method sterilization.
7. culture medium flat plate magnetic vortex type according to claim 1 is coated with the method for bacterium, it is characterized in that: the described magnetic stirring bar of C step rotates as clockwise rotating, rotate counterclockwise or suitable, counterclockwise alternate rotation.
8. according to claim 1 or 7 described culture medium flat plate magnetic vortex types are coated with the method for bacteriums, and it is characterized in that: the described magnetic stirring bar rotation time of C step is 1 ~ 2min.
9. culture medium flat plate magnetic vortex type according to claim 1 is coated with the method for bacterium, it is characterized in that: plastics or glass culture dish that described culture medium flat plate uses, the culture dish diameter is 60mm, 90mm, 100mm or 120mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100877697A CN103173392A (en) | 2013-03-19 | 2013-03-19 | Method for magnetically and rotatably applying bacteria on culture medium panel |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013100877697A CN103173392A (en) | 2013-03-19 | 2013-03-19 | Method for magnetically and rotatably applying bacteria on culture medium panel |
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| CN103173392A true CN103173392A (en) | 2013-06-26 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039156A (en) * | 2015-08-12 | 2015-11-11 | 北京市农林科学院 | Method and device suitable for water-insoluble thermostable substance uniform coating flat plate |
| CN107216997A (en) * | 2017-07-24 | 2017-09-29 | 东北林业大学 | Bacterium solution apparatus for coating and coating method |
| CN110564809A (en) * | 2019-09-18 | 2019-12-13 | 武汉迪艾斯科技有限公司 | rotary streaking inoculation method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2146491Y (en) * | 1992-08-25 | 1993-11-17 | 李建秋 | Once-used anaerobes petri-type dish |
| CN1918280A (en) * | 2004-01-22 | 2007-02-21 | 默德威特科技有限公司 | Microbial streaking device |
-
2013
- 2013-03-19 CN CN2013100877697A patent/CN103173392A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2146491Y (en) * | 1992-08-25 | 1993-11-17 | 李建秋 | Once-used anaerobes petri-type dish |
| CN1918280A (en) * | 2004-01-22 | 2007-02-21 | 默德威特科技有限公司 | Microbial streaking device |
Non-Patent Citations (2)
| Title |
|---|
| J. H. GLASSON ET AL: "Evaluation of an Automated Instrument for Inoculating and Spreading Samples onto Agar Plates.", 《JOURNAL OF CLINICAL MICROBIOLOGY》, vol. 46, no. 4, 30 April 2008 (2008-04-30) * |
| 颜学军等: "一种旋转表面涂布活菌计数方法", 《湖南医科大学学报》, vol. 15, no. 4, 31 December 1990 (1990-12-31) * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105039156A (en) * | 2015-08-12 | 2015-11-11 | 北京市农林科学院 | Method and device suitable for water-insoluble thermostable substance uniform coating flat plate |
| CN107216997A (en) * | 2017-07-24 | 2017-09-29 | 东北林业大学 | Bacterium solution apparatus for coating and coating method |
| CN110564809A (en) * | 2019-09-18 | 2019-12-13 | 武汉迪艾斯科技有限公司 | rotary streaking inoculation method |
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Application publication date: 20130626 |