CN107216997A - Bacterium solution apparatus for coating and coating method - Google Patents

Bacterium solution apparatus for coating and coating method Download PDF

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Publication number
CN107216997A
CN107216997A CN201710608428.8A CN201710608428A CN107216997A CN 107216997 A CN107216997 A CN 107216997A CN 201710608428 A CN201710608428 A CN 201710608428A CN 107216997 A CN107216997 A CN 107216997A
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China
Prior art keywords
bacterium solution
coating
disk
bottom plate
culture dish
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CN201710608428.8A
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Chinese (zh)
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CN107216997B (en
Inventor
邸雪影
于泽洋
瓮岳太
张玉洲
杨光
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Northeast Forestry University
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Northeast Forestry University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Bacterium solution apparatus for coating and coating method.It is coated needs to bacterium solution in culture dish at present to complete manually, such coating method is wasted time and energy, and is unevenly coated, and pollution is easily caused during coating.Present invention composition includes:Power set(11), fixing device is placed with above power set(1), fixing device include top plate(2), bottom plate(17), one end of top plate and bottom plate passes through connecting shaft(16)The other end of connection, top plate and bottom plate passes through fixed column(6)One group of sleeve pipe is cased with connection, connecting shaft(15), the upper and lower ends of sleeve pipe are connected with connecting shaft by bearing respectively, sleeve pipe and Petri dish box(14)One end be fixedly connected, the other end of Petri dish box is provided with stator(3), shell fragment is installed on stator(5), shell fragment is placed on the fixing groove of fixed column(4)It is interior.The present invention is used for bacterium solution apparatus for coating and coating method.

Description

Bacterium solution apparatus for coating and coating method
Technical field:
The present invention relates to a kind of bacterium solution apparatus for coating and coating method.
Background technology:
It is current during progress bacterium solution experiment, to be required for that bacterium solution is uniformly coated in culture dish until applying manually in culture dish and do, Such coating method is wasted time and energy, and is unevenly coated, and pollution is easily caused during coating, the temperature in coating process Change easily another bacterium solution loses activity, experimental result is impacted.
The content of the invention:
Waste time and energy, be unevenly coated, the problem of easily causing pollution present invention aim to address current bacterium solution coating method, There is provided a kind of coating uniform, prevent pollution, keep a kind of bacterium solution apparatus for coating and coating method of bacteria solution active.
Above-mentioned purpose is realized by following technical scheme:
A kind of bacterium solution apparatus for coating, its composition includes:Fixing device is placed with above power set, described power set, institute The fixing device stated includes top plate, bottom plate, and one end of described top plate and described bottom plate is connected by connecting shaft, described top The other end of plate and described bottom plate is connected by fixed column, is cased with one group of sleeve pipe in described connecting shaft, described sleeve pipe Upper and lower ends are connected with described connecting shaft by bearing respectively, and described sleeve pipe is fixedly connected with one end of Petri dish box, institute The other end for the Petri dish box stated, which is provided with stator, described stator, is provided with shell fragment, and described shell fragment is placed on described Fixed column fixing groove in.
Described bacterium solution apparatus for coating, described power set include having motor shaft on motor, described motor, described Motor shaft be fixedly connected with rotating disk, the left and right ends of described rotating disk are separately installed with pillar, the top of described pillar with Roller is connected by rotating shaft, has a circle groove on described bottom plate, and the upper end of described roller is located in described groove.
It is placed with described bacterium solution apparatus for coating, described Petri dish box in culture dish, described culture dish and is placed with disk, Heating tube and temperature are separately installed with 1/2nd of a diameter of described culture dish diameter of described disk, described disk Control device.
The difference in height of the pillar of described bacterium solution apparatus for coating, the pillar of described rotating disk left end and described rotating disk right-hand member For 1-3cm.
A kind of coating method of bacterium solution apparatus for coating, this method comprises the following steps:Bacterium solution is placed in culture dish by first choice, Disk is placed on culture dish centre position, pulls Petri dish box to pull, culture dish is placed in Petri dish box, pulls Petri dish box to make Obtain shell fragment to be located in fixing groove, start motor, with the rotation of motor, bottom plate carries out rugged rotation always, and disk will Bacterium solution in culture dish applies flat, and bacterium solution is maintained at steady temperature by the heating tube in disk.
Beneficial effect:
1. the present invention uses fixing device, Petri dish box can be caused to be rotated under the drive of motor, due to rotating disk left end Pillar and rotating disk right-hand member pillar difference in height be 1-3cm so that fixing device can carry out rugged uniform rotation, Disk is coated in culture dish to bacterium solution, can make it that bacterium solution coating is uniform, time saving and energy saving, improves conventional efficient.
The present invention can prevent that Petri dish box is voluntarily rotated in rotation process using the structure of shell fragment, influence culture dish The uniformity of middle bacterium solution.
Heating tube and temperature controller are separately installed with the disk of the present invention, the temperature of bacterium solution can be monitored so that The temperature of bacterium solution is maintained in certain scope, remains the activity of bacterium solution.
The present invention is provided with one group of Petri dish box, multiple culture dishes can be coated simultaneously, improve the efficiency of experiment, Save manpower.
The present invention is coated using disk, can avoid polluting bacterium solution during manual application.
Brief description of the drawings:
Accompanying drawing 1 is the structural representation of the present invention;
Accompanying drawing 2 is the A-A of present invention sectional view;
Accompanying drawing 3 is the structural representation of the disk of the present invention;
In figure:1st, fixing device;2nd, top plate;3rd, stator;4th, fixing groove;5th, shell fragment;6th, fixed column;7th, rotating shaft;8th, roller;9、 Pillar;10th, rotating disk;11st, power set;12nd, motor;13rd, motor shaft;14th, Petri dish box;15th, sleeve pipe;16th, connecting shaft;17、 Bottom plate;18th, culture dish;19th, disk;20th, heating tube;21st, temperature controller;22nd, groove.
Embodiment:
Embodiment 1:
A kind of bacterium solution apparatus for coating, its composition includes:Fixing device 1 is placed with above power set 11, described power set, Described fixing device includes top plate 2, bottom plate 17, and one end of described top plate and described bottom plate is connected by connecting shaft 16, The other end of described top plate and described bottom plate is connected by fixed column 6, and one group of sleeve pipe 15, institute are cased with described connecting shaft The upper and lower ends for the sleeve pipe stated are connected with described connecting shaft by bearing respectively, described sleeve pipe and one end of Petri dish box 14 It is fixedly connected, the other end of described Petri dish box, which is provided with stator 3, described stator, is provided with shell fragment 5, described Shell fragment is placed in the fixing groove 4 of described fixed column.
Embodiment 2:
Bacterium solution apparatus for coating according to embodiment 1, described power set include having electricity on motor 12, described motor Arbor 13, described motor shaft is fixedly connected with rotating disk 10, and the left and right ends of described rotating disk are separately installed with pillar 9, described The top of pillar be connected with roller 8 by rotating shaft 7, there is a circle groove 22, the upper end of described roller on described bottom plate In described groove.
Embodiment 3:
Culture dish 18, described culture are placed with bacterium solution apparatus for coating according to embodiment 1 or 2, described Petri dish box It is placed with disk 19 in ware, 1/2nd of a diameter of described culture dish diameter of described disk, in described disk respectively Heating tube 20 and temperature controller 21 are installed.
Embodiment 4:
Bacterium solution apparatus for coating according to embodiment 1 or 2 or 3, the pillar and described rotating disk right-hand member of described rotating disk left end Pillar difference in height be 1-3cm.
Embodiment 5:
A kind of coating method of bacterium solution apparatus for coating, this method comprises the following steps:Bacterium solution is placed in culture dish by first choice, disk Culture dish centre position is placed on, pulls Petri dish box to pull, culture dish is placed in Petri dish box, pulls Petri dish box to cause bullet Piece is located in fixing groove, starts motor, with the rotation of motor, and bottom plate carries out rugged rotation always, and disk will be cultivated Bacterium solution in ware applies flat, and bacterium solution is maintained at steady temperature by the heating tube in disk.

Claims (5)

1. a kind of bacterium solution apparatus for coating, its composition includes:Power set, it is characterized in that:It is placed with above described power set Fixing device, described fixing device includes top plate, bottom plate, and one end of described top plate and described bottom plate is connected by connecting shaft Connect, the other end of described top plate and described bottom plate is connected by fixed column, and one group of sleeve pipe, institute are cased with described connecting shaft The upper and lower ends for the sleeve pipe stated are connected with described connecting shaft by bearing respectively, and described sleeve pipe and one end of Petri dish box are solid Fixed connection, the other end of described Petri dish box, which is provided with stator, described stator, is provided with shell fragment, described shell fragment In the fixing groove for being placed on described fixed column.
2. bacterium solution apparatus for coating according to claim 1, it is characterized in that:Described power set include motor, described There is motor shaft, described motor shaft is fixedly connected with rotating disk, and the left and right ends of described rotating disk are separately installed with branch on motor Post, the top of described pillar is connected with roller by rotating shaft, has a circle groove on described bottom plate, described roller it is upper End is located in described groove.
3. bacterium solution apparatus for coating according to claim 1 or 2, it is characterized in that:Culture dish is placed with described Petri dish box, It is placed with disk in described culture dish, 1/2nd of a diameter of described culture dish diameter of described disk, described circle Heating tube and temperature controller are separately installed with piece.
4. the bacterium solution apparatus for coating according to claim 1 or 2 or 3, it is characterized in that:The pillar of described rotating disk left end and institute The difference in height of the pillar for the rotating disk right-hand member stated is 1-3cm.
5. the coating method of the bacterium solution apparatus for coating described in a kind of one of claim 1-4, it is characterized in that:This method includes as follows Step:Bacterium solution is placed in culture dish by first choice, and disk is placed on culture dish centre position, pulls Petri dish box to pull, by culture dish It is placed in Petri dish box, pulls Petri dish box that shell fragment is located in fixing groove, starts motor, with the rotation of motor, bottom plate Rugged rotation is carried out always, and the bacterium solution in culture dish is applied flat by disk, and bacterium solution is maintained at permanent by the heating tube in disk Constant temperature degree.
CN201710608428.8A 2017-07-24 2017-07-24 Bacterial liquid coating device and coating method Expired - Fee Related CN107216997B (en)

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CN107216997B CN107216997B (en) 2021-01-15

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN118240647A (en) * 2024-04-12 2024-06-25 合肥工业大学 High-density lactobacillus plantarum culturing microbial inoculum, culturing device and method

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Publication number Priority date Publication date Assignee Title
CN118240647A (en) * 2024-04-12 2024-06-25 合肥工业大学 High-density lactobacillus plantarum culturing microbial inoculum, culturing device and method

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