CN203613201U - Acellular vascular matrix gel culture medium convenient to use and having good closeness - Google Patents
Acellular vascular matrix gel culture medium convenient to use and having good closeness Download PDFInfo
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- CN203613201U CN203613201U CN201320785497.3U CN201320785497U CN203613201U CN 203613201 U CN203613201 U CN 203613201U CN 201320785497 U CN201320785497 U CN 201320785497U CN 203613201 U CN203613201 U CN 203613201U
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Abstract
The utility model relates to an acellular vascular matrix gel culture medium which is convenient to use and has good closeness. The culture medium comprises a cultivation plate provided with a base and a top cover, wherein the base is provided with a baseplate and base lateral plates; the top cover is provided with a top plate and top cover lateral plates; the base lateral plates and the top cover lateral plates are hinged through hinged blocks; an internal baffle is arranged on the baseplate, perpendicular to the top plate, circles around the baseplate a lap, and is parallel to the base lateral plates; the internal baffle is higher than the base lateral plates and embedding slots are formed between the internal baffle and the base lateral plates; cultivation columns are arranged on the baseplate in an array way and internally filled with acellular vascular matrix gel; cultivation column covers in one-to-one correspondence with the cultivation columns are arranged on the top plate; fixing pins are arranged on the top cover lateral plates; fixing holes are formed in the base lateral plates. The culture medium brings convenience for cultivation of plenty of cultures, is simple and convenient to operate, good in closeness and has low probability of pollution.
Description
Technical field
The utility model relates to biological technical field, specifically, is a kind of easy to use and good de-cell vascular stroma gel entrapment culture base of closure.
Background technology
Fibrin gel is a kind of good timbering material, is one of emphasis of Tissue Engineering Study always.Fibrin gel is mainly made up of natural extracellular matrix components, has good biocompatibility, effective biological activity and biodegradability, also has three-dimensional porous structure and good plasticity-simultaneously.In recent years, fibrin gel as timbering material increasingly extensive be applied in Tissue Engineering Study, its formation to muscular tissue, bone and cartilaginous tissue, epithelium etc. has vital role, but simultaneously can not and cartilage tissue engineeredly provide enough physical strengths for bone.
De-cell vascular stroma gel is a kind of engineered gel, and it can be applicable to following field: 1) for injecting direct treatment ischemic disease: such as lower limb ischemia, myocardial ischemia etc.; 2) as the carrier of cell therapy, after gel cell mixing, local injection treatment, plays the effect that partial restriction cell flows and runs off; 3), as laying use in endotheliocyte and the outer culturing process of level and smooth muscular cell body, help cell adhesion, maintain the effect such as cellular form and function, promotion propagation thereby play; 4) can be used as the carrier that cell three-dimensional is cultivated, Cell differentiation inducing activity etc.When for cell cultures, generally need in many containers, carry out and container is numbered, for cultivating different cells, different experiment parameter use being set, also need substratum to divide and be filled to each container.Due to common experiment, that group is set is more, and this kind of cell cultures mode brought larger inconvenience to experimenter, and easily obscures, packing substratum needs repeatedly to draw, add, operate very loaded down with trivial detailsly, and the lid of culture vessel separates with bottom, easily by outside contamination.Therefore need a kind of easy to use, de-cell vascular stroma gel entrapment culture base that closure is good badly.
Summary of the invention
The purpose of this utility model is that a kind of easy to use and good culture plate of closure is provided.
Another object of the present utility model is that a kind of easy to use and good de-cell vascular stroma gel entrapment culture base of closure is provided.
For achieving the above object, the technical scheme that the utility model is taked is:
Easy to use and the good culture plate of closure, is provided with base and top cover, and described base is provided with base plate and base side plate, and described top cover is provided with top board and top cover side plate, and described base side plate and top cover side plate are hinged by hinged block; Described base plate is provided with perpendicular to top board and around base plate one week, the Internal baffle parallel with base side plate simultaneously, and described Internal baffle is higher than base side plate, and between base side plate, forms embeded slot; Described base plate is provided with the cultivation post that array way is arranged, and described top board is provided with described cultivation post and cultivates one to one post lid; Described top cover side plate is provided with tumbler pin, and described base side plate is provided with fixed orifices; When described base and top cover fasten, described top cover side plate is inserted in embeded slot, and described cultivation post lid covers one by one to be cultivated on post, and described tumbler pin is inserted in fixed orifices.
Described hinged block comprises axle bed and two latches, and described axle bed is cylindrical, hollow tubulose, is fixed on the outer side wall of base side plate, and it is inverted "L" shaped that latch is, and two latches are mirror symmetry, is inserted in respectively in two ends of axle bed.
Described cultivation post and the distance >=1cm of base side plate, the distance >=1cm of adjacent cultivation post, described embeded slot width is than the large 0.5-1mm of roof side plate thickness.
Described cultivation post has six or 24.
Described first row is cultivated post top and is indicated row number, and described first row is cultivated post left side and indicated row number.
For realizing above-mentioned second object, the technical scheme that the utility model is taked is:
A kind of easy to use and good de-cell vascular stroma gel entrapment culture base of closure, it is provided with as above arbitrary described culture plate, de-cell vascular stroma gel entrapment culture base is housed in described cultivation post, and the height of described cell vascular stroma gel is set to cultivate the 1/3-3/5 of post height.
The utility model advantage is:
1, in base, be provided with multiple cultivation posts and indicate row number and row number, conveniently carrying out a large amount of culture cultivations and experiment grouping;
2, base and top cover are hinged, are connected as a single entity, and facilitate opening and fastening of top cover in use procedure, are difficult for obscuring simultaneously;
3, when base and top cover fasten, top cover side plate is embedded in embeded slot, cultivates post lid and covers on cultivation post, and tumbler pin and fixed orifices are fixed, and have so guaranteed base and top cover difficult drop-off, and culture and extraneous fully isolation, effectively prevented the generation of pollution;
4, the be designed to >=1cm of distance that cultivates post and base side plate, can avoid fringing effect, and the be designed to >=1cm of distance of adjacent cultivation post can prevent different the influencing each other of the interior culture of post of cultivating, and has increased the accuracy of experimental result.
Accompanying drawing explanation
Accompanying drawing 2 is the hinged schematic diagram of base and top cover.
Accompanying drawing 3 is de-cell vascular stroma gel entrapment culture based structures schematic diagram of embodiment 2.
Embodiment
Below in conjunction with embodiment and with reference to accompanying drawing, the utility model is further described.
The Reference numeral and the integral part that in accompanying drawing, relate to are as follows:
1. base, 11. base plates, 12. base side plates, 2. top cover, 21. top boards, 22. top cover side plates, 3. Internal baffle, 4. embeded slot, 5. cultivate post, row No. 61., 62. row numbers, 7. culture hole, 8. de-cell vascular stroma gel, 9. hinged block, 91. axle beds, 92. latches, 10. cultivate post lid, 101. tumbler pin pedestals, 102. tumbler pins, 103. fixed orifices pedestals, 104. fixed orificess
Please refer to Fig. 1, Fig. 1 is the de-cell vascular stroma gel entrapment culture based structures schematic diagram of embodiment 1.Described substratum is provided with base 1 and top cover 2.
Described base 1 is rectangular shape, and an opening comprises base plate 11 and base side plate 12, and described base side plate 12 was around the four edges of base plate 11 one week.Described base plate 11 is provided with Internal baffle 3, described Internal baffle 3 with perpendicular to base plate 11 and around base plate 11 1 weeks, simultaneously parallel with base side plate 12, the height of Internal baffle 3 was greater than the height of base side plate 12, and forms embeded slot 4 between base side plate 12.On described base plate 11, be also provided with six cultivation posts 5, six cultivation posts 5 are that two row's three row modes are arranged on base plate 11, on the base plate 11 of cultivation post 5 top positions of first row, all indicate row numbers 61, on the base plate 11 of cultivation post 5 leftward position of first row, all indicate row number 62.Described cultivation post 5 is cylindric, perpendicular to base plate 11, and end face opening, the inner culture hole 7 that forms of hollow.De-cell vascular stroma gel 8 is equipped with in described culture hole 7 inside, and the height of described de-cell vascular stroma gel 8 is 1/3-3/5 of culture hole 7 height.
Described top cover 2 is also rectangular shape, and an opening is provided with top board 21 and top cover side plate 22, and described top cover side plate 22 was around the four edges of top board 21 one week.
The outer side wall of the top cover side plate 22 on the outer side wall of the base side plate 12 on a long limit of described base 1 and a long limit of described top cover 2 is hinged by two hinged blocks 9.
Please refer to Fig. 2, Fig. 2 is the hinged schematic diagram of base and top cover.Each hinged block 9 comprises axle bed 91 and two latches 92.Described axle bed 91 is cylindrical, hollow tubulose, is fixed on the outer side wall of base side plate 12.Described latch 92 is fixed on top cover side plate 22, and single latch 92 is inverted "L" shaped, and two latches 92 are mirror symmetry, is inserted in respectively in two ends of axle bed 91.
Please refer to Fig. 1, described top board 21 is provided with six cultivation post lids 10 again.Described cultivation post lid 10 is cylindric, end face opening, and internal diameter equates with described cultivation post 5 external diameters, the position of six cultivation post lids 10 is corresponding one by one with the position of six described cultivation posts 5.The middle position of the top cover side plate 22 relative with hinged limit is provided with semicircular tumbler pin pedestal 101, and described tumbler pin pedestal 101 middle positions are provided with columniform tumbler pin 102.The middle position of the base side plate 12 relative with hinged limit is provided with semicircular fixed orifices pedestal 103, and described fixed orifices pedestal 103 middle positions are provided with circular fixed orifices 104.Described tumbler pin 102 matches with fixed orifices 104 sizes, just in time can be inserted in fixed orifices 104 and be difficult for deviating from.
In the time that the base 1 of de-cell vascular stroma gel entrapment culture base of the present utility model and top cover 2 fasten, described top cover side plate 22 is just in time inserted in embeded slot 4, six described cultivation post lids 10 cover one by one on six cultivation posts 5, and described tumbler pin 102 is inserted in fixed orifices 104.
The specification of the de-cell vascular stroma gel entrapment culture base of this embodiment is: described cultivation post 5 and the distance>=1cm of base side plate 12, and the distance>=1cm of adjacent cultivation post 5, described embeded slot 4 width are than the large 0.5-1mm of top cover side plate 22 thickness; The floorage of described culture hole 7 is 9.6cm
2, the height of described cultivation post lid 10 is 2-5mm.
Please refer to Fig. 2, Fig. 2 is the de-cell vascular stroma gel entrapment culture based structures schematic diagram of embodiment 2.Described substratum structure and the substratum structure of embodiment 1 are basic identical, and difference is only: it is that four row's six row modes are arranged that described base plate 11 is provided with 14 cultivation posts 5 of 24 cultivation posts 5, two on base plate 11; On described top board 21, be correspondingly provided with 24 cultivation post lids 10; The floorage of described culture hole 7 is 2cm
2.
For embodiment 1-2, it should be noted that: the design of described Internal baffle 3 is in order to form embeded slot 4 between base side plate 12, when base 1 and top cover 2 are fastened, top cover side plate 22 is just in time inserted in embeded slot 4, and the height of Internal baffle 3 is greater than the height of base side plate 12, so the inside of base 1 is formed to the space of sealing, effectively prevents the pollution of culture.Described row numbers 61 can number in order with Arabic numerals, and described row number 62 can use English alphabet to number in order successively, and the label of each cultivation post 5 like this can represent with the combination of corresponding row number and row number, and aspect is to distinguishing different experimental group.Described de-cell vascular stroma gel can be prepared according to ordinary method, as prepared in accordance with the following methods: (1) buys Adult Pig (about 120kg) aorta from slaughterhouse, after rejecting adventitia and manadesma composition, shred into irregular blood vessel small pieces, be positioned in 1% Triton X-100 solution, 37 ℃ of shaking tables, the de-cell of 200rpm concussion is processed 6.5 days, washes every other day during this time and changes 1% Triton X-100 solution until blood vessel sheet turns white.To be positioned over-80 ℃ of refrigerator freezings through de-cell blood vessel sheet after treatment spends the night and is placed on vacuum freezing drying oven (Labconco Triad 2.5L, the U.S.) lyophilize until blood vessel sheet desiccation is hard.Then be broken into powder with micromill, filtered through 60 eye mesh screens; (2) the de-cell vascular stroma powder of take respectively 50,100, preparing by 150mg above-mentioned steps (1) is positioned over the stomach en-(P7012-1G that 10ml is 0.1% containing concentration, Sigma) in 0.01mol/L hydrochloric acid soln, magnetic agitation digests in the 0.1mol/L sodium hydroxide solution that adds 1/10 volume (being 1ml) after 72 hours and hydrochloric acid, then adds 10 × PBS counterion concentration of 1/9 volume (being 1.111ml).Draw 500 μ l gelating solns and put into the 2ml syringe that reams head, put into 37 ℃ of incubators and after 1 hour, can form de-cell vascular stroma gel.The height of described cell vascular stroma gel 8 is set to the 1/3-3/5 of culture hole 7 height, can meet the demand of conventional cell cultures and research.Described base 1 and top cover 2 are hinged by hinged block 9, therefore in use directly raise top cover 2, and can not cause the top cover 2 of different experiments group to be obscured, and have avoided crossed contamination.Described hinged block 9 is designed to two latches 92 and is inserted into respectively in axle bed 91 from two ends, can guarantee hinged stability, prevents from coming off.The design of described cultivation post lid 10 can guarantee the closure of each cultivation post 5 in culturing process, has further prevented by the probability of occurrence of outside contamination and crossed contamination.Described tumbler pin 102 and the design of fixed orifices 104 can further guarantee in cell cultivation process base 1 and top cover 2 can not come off and the closure of culture space.Described cultivation post 5 and the be designed to >=1cm of distance of base side plate 12, can avoid fringing effect, and the be designed to >=1cm of distance of adjacent cultivation post 5 can prevent different the influencing each other of post 5 interior cultures of cultivating, and increases the accuracy of experimental result.Described embeded slot 4 width, than the large 0.5-1mm of top cover side plate 22 thickness, can guarantee that top cover 1 better fastens, and are difficult for dislocation, have retained again less gap simultaneously, prevent entering of pollutent.The contriver that is designed to of above numerical value draws according to experiment experience summary for many years, can significantly effectively avoid influencing each other and polluting of culture.
De-cell vascular stroma gel entrapment culture base of the present utility model in use, open top cover 2, directly add on the de-cell vascular stroma gel 8 in each cultivation post 5 wanting culturing cell, simple and convenient, remove the trouble that packing substratum and culture vessel are too much, be not easy to distinguish group from, and culturing process is difficult for polluting, and affects littlely between culture, and experimental result is accurate.
The above is only preferred implementation of the present utility model; it should be pointed out that for those skilled in the art, do not departing under the prerequisite of the utility model principle; can also make some improvement and supplement, these improvement and supplement and also should be considered as protection domain of the present utility model.
Claims (6)
1. the easy to use and good culture plate of closure, is provided with base and top cover, and described base is provided with base plate and base side plate, and described top cover is provided with top board and top cover side plate, it is characterized in that, described base side plate and top cover side plate are hinged by hinged block; Described base plate is provided with perpendicular to top board and around base plate one week, the Internal baffle parallel with base side plate simultaneously, and described Internal baffle is higher than base side plate, and between base side plate, forms embeded slot; Described base plate is provided with the cultivation post that array way is arranged, and described top board is provided with described cultivation post and cultivates one to one post lid; Described top cover side plate is provided with tumbler pin, and described base side plate is provided with fixed orifices; When described base and top cover fasten, described top cover side plate is inserted in embeded slot, and described cultivation post lid covers one by one to be cultivated on post, and described tumbler pin is inserted in fixed orifices.
2. culture plate according to claim 1, it is characterized in that, described hinged block comprises axle bed and two latches, described axle bed is cylindrical, hollow tubulose, be fixed on the outer side wall of base side plate, it is inverted "L" shaped that latch is, and two latches are mirror symmetry, is inserted in respectively in two ends of axle bed.
3. culture plate according to claim 1, is characterized in that, described cultivation post and the distance >=1cm of base side plate, and the distance >=1cm of adjacent cultivation post, described embeded slot width is than the large 0.5-1mm of roof side plate thickness.
4. culture plate according to claim 1, is characterized in that, described cultivation post has six or 24.
5. culture plate according to claim 4, is characterized in that, described first row is cultivated post top and indicated row number, and described first row is cultivated post left side and indicated row number.
6. the easy to use and good de-cell vascular stroma gel entrapment culture base of closure, it is characterized in that, it is provided with the culture plate as described in as arbitrary in claim 1-5, de-cell vascular stroma gel entrapment culture base is housed in described cultivation post, and the height of described cell vascular stroma gel is set to cultivate the 1/3-3/5 of post height.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320785497.3U CN203613201U (en) | 2013-12-04 | 2013-12-04 | Acellular vascular matrix gel culture medium convenient to use and having good closeness |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201320785497.3U CN203613201U (en) | 2013-12-04 | 2013-12-04 | Acellular vascular matrix gel culture medium convenient to use and having good closeness |
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| CN203613201U true CN203613201U (en) | 2014-05-28 |
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| CN201320785497.3U Expired - Fee Related CN203613201U (en) | 2013-12-04 | 2013-12-04 | Acellular vascular matrix gel culture medium convenient to use and having good closeness |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107216997A (en) * | 2017-07-24 | 2017-09-29 | 东北林业大学 | Bacterium solution apparatus for coating and coating method |
| CN109415678A (en) * | 2016-06-03 | 2019-03-01 | 乔治-奥古斯特-哥廷根公法基金会大学的大学医学院 | Orifice plate |
-
2013
- 2013-12-04 CN CN201320785497.3U patent/CN203613201U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415678A (en) * | 2016-06-03 | 2019-03-01 | 乔治-奥古斯特-哥廷根公法基金会大学的大学医学院 | Orifice plate |
| CN109415678B (en) * | 2016-06-03 | 2022-06-14 | 乔治-奥古斯特-哥廷根公法基金会大学的大学医学院 | Orifice plate |
| CN107216997A (en) * | 2017-07-24 | 2017-09-29 | 东北林业大学 | Bacterium solution apparatus for coating and coating method |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140528 Termination date: 20171204 |
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| CF01 | Termination of patent right due to non-payment of annual fee |