CN107216997B - Bacterial liquid coating device and coating method - Google Patents

Bacterial liquid coating device and coating method Download PDF

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Publication number
CN107216997B
CN107216997B CN201710608428.8A CN201710608428A CN107216997B CN 107216997 B CN107216997 B CN 107216997B CN 201710608428 A CN201710608428 A CN 201710608428A CN 107216997 B CN107216997 B CN 107216997B
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CN
China
Prior art keywords
culture dish
bottom plate
coating
bacterial liquid
motor
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Expired - Fee Related
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CN201710608428.8A
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Chinese (zh)
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CN107216997A (en
Inventor
邸雪影
于泽洋
瓮岳太
张玉洲
杨光
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Northeast Forestry University
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Northeast Forestry University
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Publication date
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Priority to CN201710608428.8A priority Critical patent/CN107216997B/en
Publication of CN107216997A publication Critical patent/CN107216997A/en
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Publication of CN107216997B publication Critical patent/CN107216997B/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops

Abstract

An apparatus and a method for coating a bacterial liquid. At present, the bacterial liquid in the culture dish needs to be coated manually, the coating mode wastes time and labor, the coating is uneven, and pollution is easily caused in the coating process. The invention comprises the following components: power device (11), fixing device (1) has been put to power device's top, fixing device includes roof (2), bottom plate (17), connecting axle (16) are passed through to the one end of roof and bottom plate and are connected, the other end of roof and bottom plate passes through fixed column (6) and connects, the cover has a set of sleeve pipe (15) on the connecting axle, sheathed tube upper and lower both ends pass through the bearing with the connecting axle respectively and are connected, the one end fixed connection of sleeve pipe and culture dish box (14), stationary blade (3) are installed to the other end of culture dish box, install shell fragment (5) on the stationary blade, the shell fragment is put in fixed slot (4) of fixed. The invention is used for a bacterial liquid coating device and a bacterial liquid coating method.

Description

Bacterial liquid coating device and coating method
The technical field is as follows:
the invention relates to a bacterial liquid coating device and a bacterial liquid coating method.
Background art:
when carrying out the fungus liquid experiment in the culture dish at present, all need the manual even coating of fungus liquid in the culture dish until scribbling futilely, such coating mode is wasted time and energy, and the coating is inhomogeneous, causes the pollution easily at the in-process of coating, and the change of temperature is in addition fungus liquid deactivation easily in the coating process, causes the influence to the experimental result.
The invention content is as follows:
the invention aims to solve the problems of time and labor waste, uneven coating and easy pollution caused by the conventional bacterial liquid coating mode, and provides a bacterial liquid coating device and a bacterial liquid coating method which have the advantages of uniform coating, pollution prevention and bacterial liquid activity maintenance.
The above purpose is realized by the following technical scheme:
a bacterial fluid coating device, comprising: the culture dish box comprises a power device, wherein a fixing device is placed above the power device and comprises a top plate and a bottom plate, one end of the top plate is connected with one end of the bottom plate through a connecting shaft, the other end of the top plate is connected with the other end of the bottom plate through a fixing column, a group of sleeves are sleeved on the connecting shaft, the upper end and the lower end of each sleeve are respectively connected with the connecting shaft through a bearing, the sleeves are fixedly connected with one end of the culture dish box, the other end of the culture dish box is provided with a fixing sheet, and each fixing sheet is provided with an elastic sheet which is placed in a fixing groove of the corresponding fixing column.
The fungus liquid coating device, power device include the motor, the motor on have a motor shaft, motor shaft and carousel fixed connection, the carousel about both ends install the pillar respectively, the top and the gyro wheel of pillar be connected through the pivot, the bottom plate on have a round recess, the upper end of gyro wheel be located the recess in.
Fungus liquid coating unit, the culture dish box in put the culture dish, the culture dish in put the disk, the diameter of disk be the culture dish diameter half, the disk in install heating pipe and temperature controller respectively.
The height difference between the support at the left end of the turntable and the support at the right end of the turntable is 1-3 cm.
A method for coating a bacterial liquid coating device comprises the following steps: first-selected putting the fungus liquid in the culture dish, the disk is put at the culture dish intermediate position, and pulling culture dish box pulling is put the culture dish in the culture dish box, and pulling culture dish box makes the shell fragment be located the fixed slot, and starter motor, along with the rotation of motor, the bottom plate carries out the rotation of height unevenness always, and the disk is scribbled the fungus liquid in with the culture dish, and the heating pipe in the disk keeps the fungus liquid at the constant temperature.
Has the advantages that:
1. the culture dish box can rotate under the driving of the motor by adopting the fixing device, the fixing device can uniformly rotate in an uneven manner due to the fact that the height difference between the pillar at the left end of the rotary plate and the pillar at the right end of the rotary plate is 1-3cm, the disc coats the bacterial liquid in the culture dish, the bacterial liquid can be uniformly coated, time and labor are saved, and the experiment efficiency is improved.
2. The culture dish box adopts the structure of the elastic sheet, so that the culture dish box can be prevented from rotating automatically in the rotating process, and the uniformity of bacteria liquid in the culture dish is prevented from being influenced.
3. The heating pipe and the temperature controller are respectively arranged in the wafer, so that the temperature of the bacterial liquid can be monitored, the temperature of the bacterial liquid is kept within a certain range, and the activity of the bacterial liquid is always kept.
4. The culture dish box is provided with the group of culture dish boxes, so that a plurality of culture dishes can be coated at the same time, the experiment efficiency is improved, and the labor is saved.
5. The invention uses the wafer for coating, and can avoid the pollution to the bacterial liquid in the manual coating process.
Description of the drawings:
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a cross-sectional view A-A of the present invention;
FIG. 3 is a schematic view of the structure of a wafer of the present invention;
in the figure: 1. a fixing device; 2. a top plate; 3. a fixing sheet; 4. fixing grooves; 5. a spring plate; 6. fixing a column; 7. a rotating shaft; 8. a roller; 9. a pillar; 10. a turntable; 11. a power plant; 12. a motor; 13. a motor shaft; 14. a culture dish box; 15. a sleeve; 16. a connecting shaft; 17. a base plate; 18. a culture dish; 19. a wafer; 20. heating a tube; 21. a temperature controller; 22. and (4) a groove.
The specific implementation mode is as follows:
example 1:
a bacterial fluid coating device, comprising: power device 11, power device's top put fixing device 1, fixing device include roof 2, bottom plate 17, the roof with the one end of bottom plate pass through connecting axle 16 and connect, the roof with the other end of bottom plate pass through fixed column 6 and connect, the connecting axle on the cover have a set of sleeve pipe 15, sheathed tube upper and lower both ends respectively with the connecting axle pass through the bearing and be connected, the sleeve pipe and the one end fixed connection of culture dish box 14, the other end of culture dish box install stationary blade 3, the stationary blade on install shell fragment 5, the shell fragment put fixed slot 4 of fixed column in.
Example 2:
according to embodiment 1 fungus liquid coating unit, power device include motor 12, the motor on have a motor shaft 13, motor shaft and carousel 10 fixed connection, the carousel left and right ends install pillar 9 respectively, the top of pillar be connected through pivot 7 with gyro wheel 8, the bottom plate on have a round recess 22, the upper end of gyro wheel be located the recess.
Example 3:
according to embodiment 1 or 2 fungus liquid coating unit, the culture dish box in put culture dish 18, the culture dish in put disc 19, the diameter of disc be half of the culture dish diameter, install heating pipe 20 and temperature controller 21 in the disc respectively.
Example 4:
according to embodiment 1 or 2 or 3, the height difference between the support at the left end of the turntable and the support at the right end of the turntable is 1-3 cm.
Example 5:
a method for coating a bacterial liquid coating device comprises the following steps: first-selected putting the fungus liquid in the culture dish, the disk is put at the culture dish intermediate position, and pulling culture dish box pulling is put the culture dish in the culture dish box, and pulling culture dish box makes the shell fragment be located the fixed slot, and starter motor, along with the rotation of motor, the bottom plate carries out the rotation of height unevenness always, and the disk is scribbled the fungus liquid in with the culture dish, and the heating pipe in the disk keeps the fungus liquid at the constant temperature.

Claims (2)

1. A bacterial fluid coating device, comprising: the power device is characterized in that: a fixing device is arranged above the power device and comprises a top plate and a bottom plate, one end of the top plate is connected with one end of the bottom plate through a connecting shaft, the other end of the top plate is connected with the other end of the bottom plate through a fixing column, a group of sleeves are sleeved on the connecting shaft, the upper end and the lower end of each sleeve are respectively connected with the connecting shaft through a bearing, each sleeve is fixedly connected with one end of a culture dish box, a fixing piece is arranged at the other end of each culture dish box, an elastic piece is arranged on each fixing piece, and each elastic piece is arranged in a fixing groove of the corresponding fixing column;
the power device comprises a motor, a motor shaft is arranged on the motor and fixedly connected with a turntable, supporting columns are respectively arranged at the left end and the right end of the turntable, the top ends of the supporting columns are connected with rollers through rotating shafts, a circle of groove is formed in the bottom plate, and the upper ends of the rollers are positioned in the groove;
a culture dish is arranged in the culture dish box, a circular sheet is arranged in the culture dish, the diameter of the circular sheet is one half of that of the culture dish, and a heating pipe and a temperature controller are respectively arranged in the circular sheet;
the height difference between the support column at the left end of the turntable and the support column at the right end of the turntable is 1-3cm, and the culture dish swings circumferentially.
2. The method for coating the bacteria liquid coating device according to claim 1, comprising: the method comprises the following steps: put the fungus liquid in the culture dish, the disk is put at the culture dish intermediate position, and pulling culture dish box is put the culture dish in the culture dish box, and pulling culture dish box makes the shell fragment be located the fixed slot, and starter motor, along with the rotation of motor, the bottom plate carries out the rotation of height unevenness always, and the disk is scribbled the fungus liquid in with the culture dish, and the heating pipe in the disk keeps the fungus liquid at the constant temperature.
CN201710608428.8A 2017-07-24 2017-07-24 Bacterial liquid coating device and coating method Expired - Fee Related CN107216997B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710608428.8A CN107216997B (en) 2017-07-24 2017-07-24 Bacterial liquid coating device and coating method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710608428.8A CN107216997B (en) 2017-07-24 2017-07-24 Bacterial liquid coating device and coating method

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CN107216997A CN107216997A (en) 2017-09-29
CN107216997B true CN107216997B (en) 2021-01-15

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CN201406422Y (en) * 2009-05-18 2010-02-17 郑州威瑞生物技术有限公司 Thermotank or incubator swinging mechanism
CN101812405B (en) * 2010-04-30 2013-05-22 惠倪 Polygonal plastic bag swing cell reactor and method for culturing animal cells
CN102703567B (en) * 2012-06-15 2013-10-09 江苏嘉语生物医药技术有限公司 Multi-phase co-culture and detection method and device thereof
CN103173392A (en) * 2013-03-19 2013-06-26 云南省烟草农业科学研究院 Method for magnetically and rotatably applying bacteria on culture medium panel
CN203613201U (en) * 2013-12-04 2014-05-28 上海交通大学医学院附属上海儿童医学中心 Acellular vascular matrix gel culture medium convenient to use and having good closeness
CN203678597U (en) * 2013-12-12 2014-07-02 山东科技大学 Automatic coater
CN203807458U (en) * 2014-04-25 2014-09-03 中国农业科学院兰州畜牧与兽药研究所 Rotary culture dish rack
CN104152348A (en) * 2014-07-16 2014-11-19 北京赛智创业科技有限公司 Rotary coating device and coating rod
CN204079989U (en) * 2014-09-12 2015-01-07 李红 A kind of biological culture equipment with tilting shaking table
CN104498349B (en) * 2014-12-04 2016-05-18 济南朗迈生物科技有限公司 A kind of culture medium inoculated method and apparatus that utilizes GRAVITY CONTROL dynamics
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