CN214612430U - A simple and easy board auxiliary device that scribbles for molecular cloning experiment - Google Patents

A simple and easy board auxiliary device that scribbles for molecular cloning experiment Download PDF

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Publication number
CN214612430U
CN214612430U CN202120812360.7U CN202120812360U CN214612430U CN 214612430 U CN214612430 U CN 214612430U CN 202120812360 U CN202120812360 U CN 202120812360U CN 214612430 U CN214612430 U CN 214612430U
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CN
China
Prior art keywords
box body
rotating shaft
culture dish
molecular cloning
auxiliary device
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Expired - Fee Related
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CN202120812360.7U
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Chinese (zh)
Inventor
田爱梅
崔卓越
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Xian University
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Xian University
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Priority to CN202120812360.7U priority Critical patent/CN214612430U/en
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Publication of CN214612430U publication Critical patent/CN214612430U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model discloses a simple plate coating auxiliary device for molecular cloning experiments, which comprises a box body, wherein a central rotating shaft and a rotating shaft which are rotationally connected with the box body are arranged inside the box body, a driving bevel gear which is fixedly connected with the output shaft end of a motor which is fixedly arranged at the bottom in the box body is arranged inside the box body, the driving bevel gear is meshed with a driven bevel gear which is sleeved on the central rotating shaft, a driving gear which is sleeved on the central rotating shaft is meshed with a driven gear which is sleeved on the rotating shaft, the upper end of the rotating shaft penetrates through the box body and extends to the upper part of the box body to be fixedly connected with a culture dish placing disc which is arranged above the box body, a bacteria coating rod supporting component which is fixedly connected with the box body and is used for coating a culture dish is arranged above the box body, the utility model realizes the stable rotation of the culture dish, and avoids the problem of uneven coating by hands, and the operation is more labor-saving and time-saving, so that the experimental efficiency is improved.

Description

A simple and easy board auxiliary device that scribbles for molecular cloning experiment
Technical Field
The utility model relates to a biological assay equipment technical field specifically is a simple and easy coating plate auxiliary device for molecular cloning experiment.
Background
In the traditional molecular cloning experiment, bacterial liquid is smeared in a superclean workbench in a way that a culture dish is held by hands and is smeared back and forth by elbow glass (a bacterium smearing rod), so that on one hand, the culture dish is rotated back and forth by hands, which is relatively time-consuming and labor-consuming; on the other hand, the culture dish can not keep the stability of the plane when being held by hands and rotated, so that the smearing is not uniform, some places of the grown bacterial plaques after the culture are overlapped, some places are sparse, and the spot picking cloning in the overlapped places is likely to fail in the sequencing due to the mixing, so that the test efficiency is reduced.
Disclosure of Invention
An object of the utility model is to provide a simple and easy scribble board auxiliary device for molecular cloning experiment, a serial communication port, the power distribution box comprises a box body, inside central pivot, the pivot of being connected with the box rotation of being equipped with of box, the inside output shaft end fixed connection's of motor with fixed mounting in the box of being equipped with of box initiative bevel gear, initiative bevel gear and cover are established at the epaxial driven bevel gear meshing of center pivot and are connected, the driving gear that the cover was established in the pivot of center is established and is established the epaxial driven gear meshing with the cover and be connected, the upper end of pivot pierces through the box and extends to the top of box and establishes the culture dish place set fixed connection in the box top, the box top is equipped with and is used for scribbling the fungus stick supporting component of scribbling of culture dish with box fixed connection.
Preferably, scribble fungus stick supporting component and include the bracing piece of fixed mounting in the box top, from the top down overlaps in proper order on the bracing piece and is equipped with annular installation piece, annular fixed block, the inside both sides of annular installation piece respectively fixed mounting have with the bracing piece on T type spout sliding connection's T type slider, the spacing bolt that offsets with T type spout is worn to be equipped with respectively in the outside of annular fixed block both sides, the top of annular installation piece is equipped with and passes through the fungus stick of scribbling that right angle is connected knee fixed connection with annular installation piece.
Preferably, the rotating shaft is uniformly distributed on the periphery of the central rotating shaft, and a plurality of bacteria coating rods fixedly connected with the annular mounting block through right-angle connecting bent rods are arranged above the annular mounting block along the circumferential direction.
Preferably, the number of the rotating shafts and the culture dish placing plates is the same as that of the bacteria coating rods.
Preferably, the dish is placed to the culture dish includes the carrier dish of fixed mounting at the pivot top, carrier dish top fixed mounting has the culture dish fixing base.
Preferably, the upper part of the culture dish fixing seat is provided with a notch.
Preferably, the box body is in a round table shape, and the bottom of the box body is fixedly provided with an anti-slip pad.
Compared with the prior art, the beneficial effects of the utility model are that:
1. through setting up the box, and set up central pivot in the box, the apparatus further comprises a rotating shaft, the driving gear, driven bevel gear, the initiative bevel gear, including a motor, an end cap, a controller, and a cover plate, the top setting of box scribbles fungus stick supporting component, the dish is placed to the culture dish, the steady rotation of this a culture dish of simple and easy coating auxiliary device for molecular cloning experiment has been realized, it is relatively more time-consuming to have solved to make a round trip to rotate the culture dish with the hand, hard problem, also solved because the problem of can not keep planar stability when the culture dish pivoted is held to the hand, avoid scribbling inhomogeneous problem of appearing in smearing with the hand.
2. Place the dish with pivot and culture dish, scribble the fungus stick and set up a plurality ofly respectively, realized scribbling a plurality of culture dishes simultaneously, can improve the efficiency of scribbling of culture dish to improve experimental efficiency.
Drawings
The accompanying drawings are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention, and together with the description serve to explain the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural diagram of a simple plate coating auxiliary device for molecular cloning experiments according to the present invention;
FIG. 2 is a schematic cross-sectional view of a support rod of a simple plate coating auxiliary device for molecular cloning experiments according to the present invention;
FIG. 3 is a schematic cross-sectional view of an annular mounting block of a simple plate coating auxiliary device for molecular cloning experiments according to the present invention;
FIG. 4 is a schematic structural diagram of a dish for placing a culture dish of the simple plate coating auxiliary device for molecular cloning experiments according to the present invention;
description of reference numerals:
1-box body, 2-central rotating shaft, 3-rotating shaft, 4-driving gear, 5-driven gear, 6-driven bevel gear, 7-driving bevel gear, 8-motor, 9-bacteria coating rod supporting component, 10-culture dish placing plate, 901-supporting rod, 902-annular mounting block, 903-annular fixing block, 101-bearing plate, 102-culture dish fixing seat, 904-right angle connecting bent rod, 905-bacteria coating rod, 906-T type chute, 907-T type sliding block, 908-T type sliding block and 1021-notch.
Detailed Description
In the following, the technical solutions in the embodiments of the present invention are clearly and completely described with reference to the drawings in the embodiments of the present invention, but it should be understood that the scope of the present invention is not limited by the specific embodiments.
Example (b):
as shown in fig. 1-4, a simple plate coating auxiliary device for molecular cloning experiments is characterized by comprising a box body 1, wherein a central rotating shaft 2 and a rotating shaft 3 which are rotatably connected with the box body 1 are arranged inside the box body 1, a driving bevel gear 7 fixedly connected with an output shaft end of a motor 8 fixedly arranged at the bottom in the box body 1 is arranged inside the box body 1, the driving bevel gear 7 is engaged with a driven bevel gear 6 sleeved on the central rotating shaft 2, a driving gear 4 sleeved on the central rotating shaft 2 is engaged with a driven gear 5 sleeved on the rotating shaft 3, the upper end of the rotating shaft 3 penetrates through the box body 1 and extends to the upper part of the box body 1 to be fixedly connected with a culture dish placing disc 10 arranged above the box body 1, a bacteria coating rod supporting component 9 fixedly connected with the box body 1 and used for coating a culture dish is arranged above the box body 1, and the motor 8 is controlled to drive the driving bevel gear 7 to rotate, then the driven bevel gear 6 that is connected with the meshing of initiative bevel gear 7 rotates and drives central pivot 2 and rotate and driving gear 4 rotates, driven gear 5 who is connected with the meshing of driving gear 4 rotates, thereby realize the rotation of pivot 3, pivot 3 rotates and drives the dish is placed to the culture dish and 10 rotates, can realize the steady rotation of culture dish, the fungus liquid in the culture dish can be paintd to the fungus stick supporting component 9 that scribbles that is located 1 top of box, the number of teeth that can set up driven bevel gear 6 is greater than the number of teeth of initiative bevel gear 7, can slow down motor 8, make the device's rotation more steady.
The bacteria coating rod supporting component 9 comprises a supporting rod 901 fixedly installed above the box body 1, an annular installing block 902 and an annular fixing block 903 are sequentially sleeved on the supporting rod 901 from top to bottom, T-shaped sliding blocks 907 slidably connected with T-shaped sliding grooves 906 on the supporting rod 901 are respectively and fixedly installed on two sides inside the annular installing block 902, limiting bolts 908 abutted against the T-shaped sliding grooves 906 are respectively arranged on the outer portions of two sides of the annular fixing block 903 in a penetrating mode, bacteria coating rods 905 fixedly connected with the annular installing block 902 through right-angle connecting bent rods 904 are arranged above the annular installing block 902, when the bacteria coating rods 905 in the bacteria coating rod supporting component 9 are required to be used for coating bacteria liquid, the limiting bolts 908 can be loosened so that the annular fixing block 903 moves downwards along the supporting rod 901, and then the T-shaped sliding blocks 907 on the annular installing block 902 can slide downwards along the T-shaped sliding grooves 906 on the supporting rod 901, when the bacteria-smearing bar 905 is moved down to a petri dish placed in the petri dish placing tray 10, the position of the bacteria-smearing bar 905 can be fixed by screwing the limit bolt 908.
The equipartition has a plurality ofly in the peripheral circumference of central pivot 2 for pivot 3, the top of annular installation piece 902 is equipped with a plurality ofly along the circumferencial direction and installs piece 902 through right angle connection knee 904 fixed connection with annular scribble fungus stick 905, in order to realize carrying out the paining of fungus liquid simultaneously to a plurality of culture dishes, improves experimental efficiency.
The number of the rotating shaft 3 and the culture dish placing discs 10 is consistent with that of the bacteria coating rods 905, and a plurality of culture dishes are coated at the same time.
Dish 10 is placed including the bearing dish 101 of fixed mounting at the 3 tops of pivot, bearing dish 101 top fixed mounting has culture dish fixing base 102, in order to put the culture dish card at culture dish fixing base 102, prevents to appear moving at the pivoted in-process.
The upper part of the culture dish fixing seat 102 is provided with a notch 1021, so that the culture dish is convenient to take and place.
The box 1 is round platform form, the bottom fixed mounting of box 1 has the slipmat, for making the device more steady, prevents to appear empting.
The utility model discloses a theory of operation and use flow: the simple plate coating auxiliary device for the molecular cloning experiment is placed into a sterile super clean workbench for alcohol disinfection treatment, a plurality of culture dishes with culture media prepared before are respectively clamped into a culture dish fixing seat 102, a proper amount of activated bacteria liquid is added according to the test requirement, a limit bolt 908 is unscrewed afterwards, so that an annular fixing block 903 moves downwards along a supporting rod 901, a T-shaped sliding block 907 on an annular mounting block 902 can slide downwards along a T-shaped sliding groove 906 on the supporting rod 901, when a bacteria coating rod 905 moves downwards into a culture dish placed in a culture dish placing disc 10, the limit bolt 908 can be screwed to fix the position of the bacteria coating rod 905, then a motor 8 is controlled to drive a driving bevel gear 7 to rotate, a driven bevel gear 6 meshed and connected with the driving bevel gear 7 rotates and drives a central rotating shaft 2 and a driving gear 4 to rotate, and a driven gear 5 meshed and connected with the driving gear 4 rotates, thereby realize the rotation of pivot 3, pivot 3 rotates and drives the culture dish and place a set 10 and rotate, can realize the steady rotation of culture dish, and the fungus liquid that scribbles in the culture dish that is located 1 top of box can be paintd to the fungus stick supporting component 9, will the culture dish is put and is carried out the banding after drying naturally in the superclean bench and handle, takes out at last and puts into the incubator and invert the cultivation.
Finally, the description is as follows: the above disclosure is only one specific embodiment of the present invention, however, the present invention is not limited thereto, and any changes that can be considered by those skilled in the art should fall within the protection scope of the present invention.

Claims (7)

1. The simple plate coating auxiliary device for the molecular cloning experiment is characterized by comprising a box body (1), wherein a central rotating shaft (2) and a rotating shaft (3) which are rotationally connected with the box body (1) are arranged inside the box body (1), a driving bevel gear (7) fixedly connected with an output shaft end of a motor (8) fixedly arranged at the bottom in the box body (1) is arranged inside the box body (1), the driving bevel gear (7) is in meshing connection with a driven bevel gear (6) sleeved on the central rotating shaft (2), a driving gear (4) sleeved on the central rotating shaft (2) is in meshing connection with a driven gear (5) sleeved on the rotating shaft (3), and the upper end of the rotating shaft (3) penetrates through the box body (1) and extends to the upper part of the box body (1) to be fixedly connected with a culture dish placing disc (10) arranged above the box body (1);
a bacterium coating rod supporting component (9) fixedly connected with the box body (1) and used for coating a culture dish is arranged above the box body (1).
2. The simple plate coating auxiliary device for molecular cloning experiments as claimed in claim 1, wherein: the bacteria coating rod supporting assembly (9) comprises a supporting rod (901) fixedly installed above the box body (1), an annular installing block (902) and an annular fixing block (903) are sequentially sleeved on the supporting rod (901) from top to bottom, T-shaped sliding blocks (907) in sliding connection with T-shaped sliding grooves (906) in the supporting rod (901) are fixedly installed on two sides of the inside of the annular installing block (902) respectively, and limiting bolts (908) abutted against the T-shaped sliding grooves (906) penetrate through the outer portions of two sides of the annular fixing block (903) respectively;
and a bacterium coating rod (905) fixedly connected with the annular mounting block (902) through a right-angle connecting bent rod (904) is arranged above the annular mounting block (902).
3. The simple plate coating auxiliary device for molecular cloning experiments as claimed in claim 2, wherein: the periphery circumference equipartition of pivot (3) in central pivot (2) has a plurality ofly, the top of annular installation piece (902) is equipped with a plurality ofly along the circumferencial direction and installs piece (902) and pass through right angle connection knee (904) fixed connection's scribble fungus stick (905).
4. The simple plate coating auxiliary device for molecular cloning experiments as claimed in claim 3, wherein: the number of the rotating shaft (3) and the culture dish placing plate (10) is consistent with that of the bacteria coating rods (905).
5. The simple plate coating auxiliary device for molecular cloning experiments as claimed in claim 1, wherein: dish is placed dish (10) and is included bearing dish (101) of fixed mounting at pivot (3) top, bearing dish (101) top fixed mounting has culture dish fixing base (102).
6. The simple plate coating auxiliary device for molecular cloning experiments as claimed in claim 5, wherein: the upper part of the culture dish fixing seat (102) is provided with a notch (1021).
7. The simple plate coating auxiliary device for molecular cloning experiments as claimed in claim 1, wherein: the box body (1) is in a round table shape, and the bottom of the box body (1) is fixedly provided with an anti-slip pad.
CN202120812360.7U 2021-04-20 2021-04-20 A simple and easy board auxiliary device that scribbles for molecular cloning experiment Expired - Fee Related CN214612430U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120812360.7U CN214612430U (en) 2021-04-20 2021-04-20 A simple and easy board auxiliary device that scribbles for molecular cloning experiment

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120812360.7U CN214612430U (en) 2021-04-20 2021-04-20 A simple and easy board auxiliary device that scribbles for molecular cloning experiment

Publications (1)

Publication Number Publication Date
CN214612430U true CN214612430U (en) 2021-11-05

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Application Number Title Priority Date Filing Date
CN202120812360.7U Expired - Fee Related CN214612430U (en) 2021-04-20 2021-04-20 A simple and easy board auxiliary device that scribbles for molecular cloning experiment

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108977351A (en) * 2018-10-29 2018-12-11 西北农林科技大学 The automatic bacterium solution spreader of use for laboratory

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108977351A (en) * 2018-10-29 2018-12-11 西北农林科技大学 The automatic bacterium solution spreader of use for laboratory
CN108977351B (en) * 2018-10-29 2024-05-03 西北农林科技大学 Automatic bacterial liquid coater for laboratory

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20211105

CF01 Termination of patent right due to non-payment of annual fee