CN217709476U

CN217709476U - Device for counting microorganism flat plates

Info

Publication number: CN217709476U
Application number: CN202221430237.XU
Authority: CN
Inventors: 郭芳坤; 胡玉金; 关志勇; 史新会
Original assignee: Shandong Tianrunhe Bioengineering Co ltd
Current assignee: Shandong Tianrunhe Bioengineering Co ltd
Priority date: 2022-06-09
Filing date: 2022-06-09
Publication date: 2022-11-01
Anticipated expiration: 2032-06-09

Abstract

The utility model relates to a dull and stereotyped device for count of microorganism, which comprises a tray, the drainage piece, culture dish and bearing structure, bearing structure includes the base, pillar and support, the tray sets up the top at the base, the pillar vertical fixation sets up the one side at the base, the one end and the pillar fixed connection of support, the other end and the drainage piece of support are connected, and support parallel arrangement is in the top of base, be provided with the motor in the base, the output of motor runs through the bottom surface fixed connection of base and tray, the tray upper surface is provided with fixed subassembly, the culture dish can be dismantled with fixed subassembly and be connected, the drainage piece, the culture dish, the center of tray and motor output is on an axis. The utility model discloses a rotatory tray produces centrifugal force, and the drive fungus liquid has avoided fungus liquid, culture medium to be stained with on the culture dish lateral wall from the even outdiffusion in center on the culture medium surface, has reduced the risk that the exogenous bacterium pollutes.

Description

Device for counting microorganism flat plates

Technical Field

The utility model relates to a microorganism count technical field, in particular to device is used in dull and stereotyped count of microorganism.

Background

At present, two methods, namely a dilution coating plate method and a pouring method, are generally adopted for plate counting of microorganisms (bacteria). The dilution coating plate method is a commonly used bacteria counting method in which uniform coating using a coating rod requires an appropriate bacterial droplet addition amount and coating is repeated. The repeated coating is the key to the uniform distribution of the bacterial cells, but the increase of the coating time prolongs the opening time of the culture dish, and the probability of pollution is increased. At the same time, the coating is also not uniform due to the limitation of the skill of the operator.

Decantation is commonly used for anaerobic culture of bacteria. When the pouring method is used for viable bacteria counting, in order to fully mix the culture medium and the bacteria liquid, the culture medium is required to be shaken after being added, and the operation easily causes that the bacteria-bearing culture medium is stained to the side edge of the culture dish close to the opening position, so that pollution is easily caused or bacteria grow on the side of the culture dish and counting is difficult; meanwhile, the shaking operation takes a certain time, which easily causes the unused culture medium to be cooled and not to be used continuously, and is not suitable for continuous operation. Both methods are limited by the skill of individual operation, and problems of uneven coating, bacterial cells or culture medium sticking to the side of the culture dish, and the like often occur.

SUMMERY OF THE UTILITY MODEL

In order to solve the problems in the prior art, the utility model provides a device for counting microorganism plates.

The utility model provides a pair of dull and stereotyped device for count of microorganism adopts following technical scheme:

the utility model provides a dull and stereotyped device for count of microorganism, includes tray, drainage piece, culture dish and bearing structure, bearing structure includes base, pillar and support, the tray sets up the top at the base, pillar vertical fixation sets up the one side at the base, the one end and the pillar fixed connection of support, the other end and the drainage piece of support are connected, just support parallel arrangement is in the top of base, be provided with the motor in the base, the output of motor runs through the bottom surface fixed connection of base and tray, the tray upper surface is provided with fixed subassembly, the culture dish can be dismantled with fixed subassembly and be connected, the center of drainage piece, culture dish, tray and motor output is on an axis.

By adopting the technical scheme, the rotary tray is adopted to generate centrifugal force, so that the bacterial liquid is driven to uniformly diffuse outwards from the center on the surface of the culture medium in the culture dish, the bacterial liquid and the culture medium are prevented from being attached to the side wall of the culture dish, and the risk of exogenous bacterial pollution is reduced; the use of mechanical equipment reduces the personnel operation of the coating process, and has lower requirements on the proficiency of the operators.

Preferably, the drainage piece is a hollow cone, a first round hole is formed in the tip of the bottom of the hollow cone, and the tray is a circular tray.

Preferably, a disposable hollow cone matched with the drainage piece is arranged in the drainage piece, a round hole II matched with the round hole I is arranged at the tip of the bottom of the disposable hollow cone, and the diameter of the round hole I is larger than that of the round hole II.

By adopting the technical scheme, the disposable hollow cone is a disposable article and is directly contacted with the bacteria liquid and replaced along with the replacement of the bacteria liquid.

Preferably, the fixing assembly includes a first fixing member and a second fixing member, and the first fixing member and the second fixing member are symmetrically disposed about a center of the tray.

Through adopting above-mentioned technical scheme, fix the culture dish.

Preferably, first mounting includes first grip block, first backup pad and first threaded rod, the bottom surface of first grip block and the top surface sliding connection of tray, first backup pad sets up the one side of keeping away from the second mounting at first grip block, the bottom surface and the tray fixed connection of first backup pad, be provided with the screw hole in the first backup pad, the one end through thread hole and the first grip block fixed connection of first threaded rod.

Preferably, the second fixing part comprises a second clamping plate, a second supporting plate and a second threaded rod, the bottom surface of the second clamping plate is connected with the top surface of the tray in a sliding mode, the second supporting plate is arranged on one side, away from the first fixing part, of the second clamping plate, the bottom surface of the second supporting plate is fixedly connected with the tray, a threaded hole is formed in the second supporting plate, and one end of the second threaded rod penetrates through the threaded hole and is fixedly connected with the second clamping plate.

Through adopting above-mentioned technical scheme, make things convenient for the culture dish to install and dismantle on the tray.

Preferably, the anti-skidding rubber mats are arranged on the side, close to each other, of each of the first clamping plate and the second clamping plate.

To sum up, the utility model discloses following useful technological effect has:

1. the utility model discloses a rotatory tray produces centrifugal force, and the even outdiffusion in the culture medium surface of drive fungus liquid in the culture dish from the center has avoided fungus liquid, culture medium to be stained with on the culture dish lateral wall, has reduced the risk that the external source fungus pollutes.

2. The utility model discloses simple structure, convenient operation has reduced the personnel's operation of coating process through mechanical equipment's use, and is lower to operating personnel proficiency requirement.

Drawings

FIG. 1 is a schematic front view of a device for counting microorganism plates according to the present invention;

FIG. 2 is a schematic top view of the apparatus for counting microorganism plates according to the present invention;

FIG. 3 is a schematic top view of a first fixing member of the apparatus for counting microorganism plates according to the present invention;

FIG. 4 is a schematic top view of a second fixing member of the apparatus for counting microorganism plates according to the present invention;

FIG. 5 is a schematic view of the first fixing member of the apparatus for counting microorganism plates according to the present invention.

Wherein, 1, a tray; 2. a drainage member; 3. a culture dish; 4. a support structure; 401. a base; 402. a pillar; 403. a support; 5. a disposable hollow cone; 6. a first fixing member; 601. a first clamping plate; 602. a first support plate; 603. a first threaded rod; 7. a second fixing member; 701. a second clamping plate; 702. a second support plate; 703. a second threaded rod; 8. an antiskid rubber pad.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings.

The embodiment of the utility model discloses dull and stereotyped device for count of microorganism. Referring to fig. 1, including tray 1, drainage piece 2, culture dish 3 and bearing structure 4, bearing structure 4 includes base 401, pillar 402 and support 403, tray 1 sets up the top at base 401, pillar 402 vertical fixation sets up the one side at base 401, the one end and the pillar 402 fixed connection of support 403, the other end and the drainage piece 2 of support 403 are connected, just support 403 parallel arrangement is in the top of base 401, drainage piece 2 is hollow circular cone, the bottom pointed end of hollow circular cone is provided with round hole one, tray 1 is circular tray. The drainage piece 2 is internally provided with a disposable hollow cone 5 matched with the drainage piece, the tip of the bottom of the disposable hollow cone 5 is provided with a round hole II matched with the round hole I, and the diameter of the round hole I is larger than that of the round hole II.

Be provided with the motor in the base 401, the output of motor runs through the bottom surface fixed connection of base 401 and tray 1, tray 1 upper surface is provided with fixed subassembly, culture dish 3 can dismantle with fixed subassembly and be connected, the center of drainage piece 2, culture dish 3, tray 1 and motor output is on an axis. The fixing assembly comprises a first fixing member 6 and a second fixing member 7, and the first fixing member 6 and the second fixing member 7 are symmetrically arranged about the center of the tray 1. First mounting 6 includes first grip block 601, first backup pad 602 and first threaded rod 603, the bottom surface of first grip block 601 and the top surface sliding connection of tray 1, first backup pad 602 sets up the one side of keeping away from second mounting 7 at first grip block 601, the bottom surface and the tray 1 fixed connection of first backup pad 602, be provided with the screw hole on the first backup pad 602, the one end through thread hole and the first grip block 601 fixed connection of first threaded rod 603. The second fixing part 7 comprises a second clamping plate 701, a second supporting plate 702 and a second threaded rod 703, the bottom surface of the second clamping plate 701 is in sliding connection with the top surface of the tray 1, the second supporting plate 702 is arranged on one side, away from the first fixing part 6, of the second clamping plate 701, the bottom surface of the second supporting plate 702 is fixedly connected with the tray 1, a threaded hole is formed in the second supporting plate 702, and one end of the second threaded rod 703 penetrates through the threaded hole and is fixedly connected with the second clamping plate 701. The anti-skid rubber mat 8 is arranged on one side, close to each other, of the first clamping plate 601 and the second clamping plate 701.

When in use: in an aseptic operation platform, after preparing a bacterial liquid diluent and a culture dish 3 with a culture medium according to the operation requirements of a dilution coating flat plate method, fixing the culture dish 3 on a tray 1, and enabling the center of the culture dish 3 to coincide with the center of the tray 1. The first fixing piece 6 and the second fixing piece 7 which are symmetrically arranged on the tray 1 fix the culture dish 3 placed on the tray 1, after the culture dish 3 is placed, the first threaded rod 603 and the second threaded rod 703 are screwed respectively, the first clamping plate 601 and the second clamping plate 701 are pushed to move towards the culture dish 3 until the culture dish 3 is clamped tightly, one surface of the first clamping plate 601 and the second clamping plate 701, which is in contact with the culture dish 3, is adapted to the culture dish 3, and the circle center of the culture dish 3 and the circle center of the tray 1 are on the same axis by adjusting the screwing-in length of the first threaded rod 603 and the second threaded rod 703. The first clamping plate 601 and the second clamping plate 701 are provided with anti-skid rubber pads 8 on the surfaces contacting with the culture dish 3.

After the culture dish 3 is fixed, the disposable hollow cone 5 is placed in the drainage piece 2, and the disposable hollow cone and the drainage piece can be uniformly attached. The drainage piece 2 is a hollow cone, a first round hole is formed in the top end of the bottom of the hollow cone, a second round hole matched with the first round hole is formed in the top end of the bottom of the disposable hollow cone 5, and the first round hole and the second round hole are used for guiding bacterial liquid to be dripped to the central point of the culture dish 3. The axial lead of the output end of the motor, the circle center of the tray 1, the circle center of the culture dish 3, and the centers of the first round hole and the second round hole are on the same vertical line. The disposable hollow cone 5 is a disposable article and is directly contacted with the bacteria liquid and replaced along with the replacement of the bacteria liquid. The motor sets up in base 401, and the output of motor runs through the center of base 401's top surface connection tray 1 bottom, drives tray 1 and rotates.

A pipette gun is used for sucking bacteria with a certain volume which is diluted and dropping the bacteria into a disposable hollow cone 5, the bacteria is dropped into the center of a culture medium (culture dish) below from a circular hole at the top end of the cone along the vertical direction, then a motor drives a tray 1 to rotate at a constant speed, the bacteria uniformly diffuses on the surface of the culture medium in the culture dish 3 in a sector mode, when the bacteria diffuses to a position close to the edge, the motor is turned off, and the rotation is stopped. And (3) rotationally screwing out the first threaded rod 603 and the second threaded rod 703, taking out the culture dish 3, and covering the culture dish on the other surface to perform static culture. The other dish and disposable hollow cone can then be replaced and the procedure repeated. In order to avoid the bacteria liquid from diffusing too fast on the surface of the culture medium, a certain amount of thickening material, such as glycerol, white sugar, agar, etc. with proper concentration can be added when the bacteria liquid is diluted to a specific gradient.

When the pouring method is adopted, the disposable hollow cone 5 with the round hole II with the larger diameter is used instead, a mixed solution of a specific amount of bacterial liquid and an uncooled liquid culture medium is added to the disposable hollow cone 5, and the mixed solution is uniformly distributed in the culture dish through a uniform dripping process. After the conventional pouring method or the pouring method is finished, water agar or a sterile culture medium is dripped at the disposable hollow cone 5 and covered on the surface of a culture medium containing bacteria in a flat plate through the device, so that a thin water agar layer or a culture medium layer without bacteria is covered on the surface of the culture medium in a culture dish, the anaerobic growth environment of all viable bacteria is realized, and the condition that the viable bacteria on the surface of the culture medium contact air is avoided.

The invention adopts the rotating tray 1 to generate centrifugal force, drives the bacterial liquid to uniformly diffuse outwards from the center on the surface of the culture medium, and controls the rotating speed and time of the motor to ensure that the bacterial liquid is uniformly distributed on the surface of the culture medium without being excessively stuck to the outer wall of a culture dish 3 to cause pollution and lower counting result; the requirement on the addition amount of the bacterial liquid is relatively wide, 100-300 mu L of bacterial liquid can be uniformly coated by dripping the bacterial liquid into a culture dish 3 with the diameter of 90mm, the problem that the coating time is increased along with the increase of the dripping amount of the bacterial liquid in the coating process of a traditional coating rod is avoided, the personnel operation in the coating process is reduced, and the requirement on the proficiency of operators is low; can fully ensure the uniform distribution of the bacterial liquid on the culture medium.

Above is the preferred embodiment of the utility model, not limit according to this the utility model discloses a protection scope, the event: all equivalent changes made according to the structure, shape and principle of the utility model are covered within the protection scope of the utility model.

Claims

1. A device for counting microorganism plates is characterized in that: including tray (1), drainage piece (2), culture dish (3) and bearing structure (4), bearing structure (4) include base (401), pillar (402) and support (403), tray (1) sets up the top at base (401), pillar (402) vertical fixation sets up the one side at base (401), the one end and pillar (402) fixed connection of support (403), the other end and the drainage piece (2) of support (403) are connected, just support (403) parallel arrangement are in the top of base (401), be provided with the motor in base (401), the output of motor runs through the bottom surface center fixed connection of base (401) and tray (1), tray (1) upper surface is provided with fixed subassembly, culture dish (3) can be dismantled with fixed subassembly and be connected, the center of drainage piece (2), culture dish (3), tray (1) and motor output is on an axis.

2. The apparatus for counting microorganism plates according to claim 1, wherein: the drainage piece (2) is a hollow cone, a first round hole is formed in the tip of the bottom of the hollow cone, and the tray (1) is a circular tray.

3. The apparatus for counting microorganism plates according to claim 2, wherein: the drainage piece (2) is internally provided with a disposable hollow cone (5) matched with the drainage piece, the tip of the bottom of the disposable hollow cone (5) is provided with a round hole II matched with the round hole I, and the diameter of the round hole I is larger than that of the round hole II.

4. The apparatus for counting microorganism plates according to claim 1, wherein: the fixing assembly comprises a first fixing piece (6) and a second fixing piece (7), and the first fixing piece (6) and the second fixing piece (7) are symmetrically arranged relative to the center of the tray (1).

5. The apparatus for counting microorganism plates according to claim 4, wherein: first mounting (6) are including first grip block (601), first backup pad (602) and first threaded rod (603), the bottom surface of first grip block (601) and the top surface sliding connection of tray (1), first backup pad (602) sets up the one side of keeping away from second mounting (7) in first grip block (601), the bottom surface and tray (1) fixed connection of first backup pad (602), be provided with the screw hole on first backup pad (602), the one end through thread hole and first grip block (601) fixed connection of first threaded rod (603).

6. The apparatus for counting microorganism plates according to claim 5, wherein: the second fixing piece (7) comprises a second clamping plate (701), a second supporting plate (702) and a second threaded rod (703), the bottom surface of the second clamping plate (701) is in sliding connection with the top surface of the tray (1), the second supporting plate (702) is arranged on one side, away from the first fixing piece (6), of the second clamping plate (701), the bottom surface of the second supporting plate (702) is fixedly connected with the tray (1), a threaded hole is formed in the second supporting plate (702), and one end of the second threaded rod (703) penetrates through the threaded hole and is fixedly connected with the second clamping plate (701).

7. The apparatus for counting microorganism plates according to claim 6, wherein: and the mutually close sides of the first clamping plate (601) and the second clamping plate (701) are provided with anti-skidding rubber pads (8).