CN108977351B - Automatic bacterial liquid coater for laboratory - Google Patents

Automatic bacterial liquid coater for laboratory Download PDF

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Publication number
CN108977351B
CN108977351B CN201811270548.2A CN201811270548A CN108977351B CN 108977351 B CN108977351 B CN 108977351B CN 201811270548 A CN201811270548 A CN 201811270548A CN 108977351 B CN108977351 B CN 108977351B
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China
Prior art keywords
connecting rod
crank
rotary motion
glass stick
laboratory
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CN108977351A (en
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董金城
姜道强
崔成成
单德旭
寇小希
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Northwest A&F University
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Northwest A&F University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/02Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model provides a main characteristics of this automatic high performance fungus liquid coating ware is used in laboratory that this fungus coating ware is mainly characterized by using a power supply to realize the linear reciprocating motion of glass stick and the gyration motion of culture dish simultaneously, and the speed cooperation is reasonable, and the operation of automatic coating is accomplished to accuracy, simple structure, exquisite small and exquisite, convenient operation, and the cost is lower, is fit for middle-size and small-size laboratory.

Description

Automatic bacterial liquid coater for laboratory
Technical Field
The invention realizes automatic bacterial liquid coating by utilizing a mechanical structure and a motor.
Background
It is well known that in most microbiological operations, the step of counting microorganisms is required, and the most common method at present is the dilution plate counting method. Bacterial liquid coating is a basic operation in a dilution plate counting method, and is used for years because of being manually operated by an experimenter, simple in operation, strong in feasibility and reliability. However, conventional manual operations also expose a number of problems, such as: the operation is complicated, time-consuming and manual operation efficiency is low, the uniformity of the coated flat plate cannot be guaranteed, only one flat plate can be used at a time, the experimental process is greatly prolonged, the experimental accuracy is affected, and the like. The manual operation is suitable for small-batch work, and if a large number of flat coating works need to be completed at one time, a large amount of manpower, material resources and time need to be arranged for completion, and the time and the labor are wasted. Therefore, the machine replaces manual operation, the working efficiency is improved, and the design of the automatic plate coating machine is particularly important.
There are also some automatic bacterial liquid applicators abroad at present. Such as a fully automated plating machine manufactured by the French/bioMerieux (Mei Liai) design and a DW-16 automated bacterial coating inoculating machine manufactured by the Hangzhou macrodesign. Although they can better solve the problems of tedious and time-consuming bacteria coating process. But the full-automatic flat inoculation instrument has larger size, occupies large space, is suitable for large-scale laboratories with large-scale and multi-colony researches, has large sample quantity required in the experimental process and large power consumption in the using process, and has too high price for small and medium-sized laboratories; the DW-16 automatic bacteria coating inoculating instrument needs manual operation to assist, and generally needs to be coated one by one, so that the working efficiency is not improved obviously. Meanwhile, when the specimen diffuses in the rotation of the culture dish, the probability of accumulation of specimen liquid at the edge and the corner of the culture dish is increased, the difficulty is increased for the colony counting process, and finally the influence of experimental data is not suitable for small and medium-sized laboratories. Therefore, the utility model has the problem of being usable and not usable, and the practicability is not high.
Disclosure of Invention
The invention mainly designs a light and simple automatic bacterial liquid coater for laboratory operation. The bacterial liquid coater is mainly characterized in that a motor outputs rotary motion, and simultaneously, the rotary motion of four culture dishes on a chassis and the linear reciprocating motion of a glass rod are realized, so that the bacterial liquid is uniformly coated automatically. The design aims at reducing the labor intensity and improving the bacteria coating efficiency, and the design principle is that the rotary motion of a motor is changed into the linear reciprocating motion of a glass rod by utilizing a crank-like slide block mechanism, and meanwhile, the rotary motion of a main shaft is transmitted to a chassis by utilizing four gears, so that the rotary motion of a culture dish is realized. The whole mechanism only needs one power source, has simple structure, is exquisite and small, is convenient to operate, has lower cost and is suitable for small and medium-sized laboratories.
The invention has the characteristics and the application that: the bacterial liquid coater realizes bacterial liquid coating by using a simple crank-like slider mechanism and a gear, and well solves the problems of long time consumption, tedious work and expensive instrument of bacterial coating in a small and medium laboratory.
Description of the drawings:
FIG. 1 is a schematic diagram of the whole bacteria liquid coater
Detailed Description
The present invention will be described in further detail with reference to the accompanying drawings.
The specific operation process is as follows:
The motor [1] outputs rotary motion, the crank [2] is driven to move, the eccentric shaft of the crank [2] is embedded into the hollow groove of the connecting rod [3], the connecting rod [3] is driven to linearly reciprocate along the linear guide rail [4], the glass rod connecting rod [5] is fixed on the connecting rod [3], and the glass rod [6] is connected to the glass rod connecting rod [5] by the rubber tube [7], so that the linear reciprocation of the glass rod [6] is realized. The lower end of the crank (2) is connected with a large gear (8) at the bottom of the device, and the movement is uniformly distributed to four small gears (9) meshed with the large gear to drive a culture dish tray (10) fixedly connected with the large gear to realize low-speed rotation of the culture dish, and finally, the glass rod (6) is subjected to operation similar to manual coating by combining linear movement and rotary movement on the culture dish tray (10).

Claims (1)

1. The utility model provides an automatic fungus liquid coating ware for laboratory, a serial communication port, utilize the rotary motion of power supply motor (1) output, drive and its coaxial coupling's crank (2) rotate, the eccentric shaft portion of crank (2) is embedded in the hollow inslot of connecting rod (3), the round hole at connecting rod (3) both ends overlaps respectively on two linear guide (4), realize turning into the rectilinear reciprocating motion of connecting rod (3) along linear guide (4) with the rotary motion of crank (2), glass stick connecting rod (5) are linked firmly respectively at connecting rod (3) both ends, glass stick connecting rod (5) are through the rubber tube (7) of convenient to dismantle, and then realize the rectilinear reciprocating motion of glass stick (6), crank (2) lower extreme and gear wheel (8) coaxial coupling, evenly distributed four pinion (9) and drive their rotation all around, pinion (9) continue to drive and link to do rotary motion in culture dish tray (10) of upper end, finally realize glass stick (6) and do the rectilinear coating operation that combines with rotary motion on culture dish (10).
CN201811270548.2A 2018-10-29 2018-10-29 Automatic bacterial liquid coater for laboratory Active CN108977351B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201811270548.2A CN108977351B (en) 2018-10-29 2018-10-29 Automatic bacterial liquid coater for laboratory

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Application Number Priority Date Filing Date Title
CN201811270548.2A CN108977351B (en) 2018-10-29 2018-10-29 Automatic bacterial liquid coater for laboratory

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CN108977351A CN108977351A (en) 2018-12-11
CN108977351B true CN108977351B (en) 2024-05-03

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024108395A1 (en) * 2022-11-22 2024-05-30 深圳先进技术研究院 Microbial liquid coating device and coating method therefor

Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3962040A (en) * 1974-03-14 1976-06-08 The United States Of America As Represented By The Department Of Health, Education And Welfare Method and apparatus for plating and counting aerobic bacteria
JPH09187268A (en) * 1996-01-11 1997-07-22 Nishi Nippon Shinku Tank Kk Device for analyzing bacteria
CN2673078Y (en) * 2003-10-24 2005-01-26 连云港国鑫医药设备有限公司 Liquid bacterial culture inoculating device
FR2876385A1 (en) * 2004-10-12 2006-04-14 Interscience Sarl Inoculator for depositing biological solutions in a culture medium comprises distribution of the solution by using an inoculating arm with a tubular element/stylet that delivers the solution above the medium recipient
CN104152348A (en) * 2014-07-16 2014-11-19 北京赛智创业科技有限公司 Rotary coating device and coating rod
CN204052053U (en) * 2014-07-16 2014-12-31 北京赛智创业科技有限公司 A kind of rotary coating device and pallet
CN205398628U (en) * 2016-03-01 2016-07-27 新奥科技发展有限公司 A vaccinate device automatically for declining algae solid culture
CN105886383A (en) * 2016-06-14 2016-08-24 重庆庞通医疗器械有限公司 Microorganism streak inoculation instrument
CN205687934U (en) * 2016-06-14 2016-11-16 重庆庞通医疗器械有限公司 Gear driven type microorganism streak inoculation instrument
CN206266554U (en) * 2016-12-06 2017-06-20 邯郸学院 A kind of microbial inoculant device of knee-piece
CN206591120U (en) * 2017-03-13 2017-10-27 苏州金唯智生物科技有限公司 A kind of apparatus for coating
CN109694818A (en) * 2017-10-23 2019-04-30 四川农业大学 A kind of microculture plate apparatus for coating
CN209049287U (en) * 2018-10-19 2019-07-02 高鸿章 A kind of medical microbial dilution mixing device
CN209481670U (en) * 2018-10-29 2019-10-11 西北农林科技大学 The automatic bacterium solution spreader of use for laboratory
CN214612430U (en) * 2021-04-20 2021-11-05 西安文理学院 A simple and easy board auxiliary device that scribbles for molecular cloning experiment
CN216274105U (en) * 2021-08-02 2022-04-12 西北农林科技大学 Small semi-automatic bacteria liquid coating machine

Patent Citations (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3962040A (en) * 1974-03-14 1976-06-08 The United States Of America As Represented By The Department Of Health, Education And Welfare Method and apparatus for plating and counting aerobic bacteria
JPH09187268A (en) * 1996-01-11 1997-07-22 Nishi Nippon Shinku Tank Kk Device for analyzing bacteria
CN2673078Y (en) * 2003-10-24 2005-01-26 连云港国鑫医药设备有限公司 Liquid bacterial culture inoculating device
FR2876385A1 (en) * 2004-10-12 2006-04-14 Interscience Sarl Inoculator for depositing biological solutions in a culture medium comprises distribution of the solution by using an inoculating arm with a tubular element/stylet that delivers the solution above the medium recipient
CN104152348A (en) * 2014-07-16 2014-11-19 北京赛智创业科技有限公司 Rotary coating device and coating rod
CN204052053U (en) * 2014-07-16 2014-12-31 北京赛智创业科技有限公司 A kind of rotary coating device and pallet
CN205398628U (en) * 2016-03-01 2016-07-27 新奥科技发展有限公司 A vaccinate device automatically for declining algae solid culture
CN105886383A (en) * 2016-06-14 2016-08-24 重庆庞通医疗器械有限公司 Microorganism streak inoculation instrument
CN205687934U (en) * 2016-06-14 2016-11-16 重庆庞通医疗器械有限公司 Gear driven type microorganism streak inoculation instrument
CN206266554U (en) * 2016-12-06 2017-06-20 邯郸学院 A kind of microbial inoculant device of knee-piece
CN206591120U (en) * 2017-03-13 2017-10-27 苏州金唯智生物科技有限公司 A kind of apparatus for coating
CN109694818A (en) * 2017-10-23 2019-04-30 四川农业大学 A kind of microculture plate apparatus for coating
CN209049287U (en) * 2018-10-19 2019-07-02 高鸿章 A kind of medical microbial dilution mixing device
CN209481670U (en) * 2018-10-29 2019-10-11 西北农林科技大学 The automatic bacterium solution spreader of use for laboratory
CN214612430U (en) * 2021-04-20 2021-11-05 西安文理学院 A simple and easy board auxiliary device that scribbles for molecular cloning experiment
CN216274105U (en) * 2021-08-02 2022-04-12 西北农林科技大学 Small semi-automatic bacteria liquid coating machine

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