CN101940124B - Continuous quantitative adjustable inoculation gun for edible fungi - Google Patents

Continuous quantitative adjustable inoculation gun for edible fungi Download PDF

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Publication number
CN101940124B
CN101940124B CN200910117351XA CN200910117351A CN101940124B CN 101940124 B CN101940124 B CN 101940124B CN 200910117351X A CN200910117351X A CN 200910117351XA CN 200910117351 A CN200910117351 A CN 200910117351A CN 101940124 B CN101940124 B CN 101940124B
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China
Prior art keywords
piston
core case
check valve
spring
valve body
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CN200910117351XA
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CN101940124A (en
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路等学
王龙
王秉峰
赵玉卉
张铎
秦鹏
高静梅
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Institute of Biology of Gansu Academy of Sciences
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Institute of Biology of Gansu Academy of Sciences
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Abstract

The invention discloses a continuous quantitative adjustable inoculation gun for edible fungi, which comprises a hollow shell, a piston and a handle, wherein the piston is positioned in the shell; the handle is connected with the shell; a through hole is arranged in the piston; the front end of the piston is provided with a seal groove; a seal ring is arranged in the seal groove; the back end of the piston is provided with a fungus liquid inlet device; the front end of the piston is provided with a fungus liquid outlet device; the back end of the piston is provided with a thread; and the side face of the tail of the piston is provided with scales. The invention has the advantages of simple and convenient operation, high inoculation speed, quantitative and adjustable inoculum size, no pollution, high efficiency and the like, obviously reduces the manual labor intensity, can integrally carry out high pressure steam sterilization, can satisfy the requirements for industrial production, and is suitable for family workshop type production.

Description

The adjustable inoculating gun of edible mushroom continuous and quantitative
Technical field
The present invention relates to a kind of edible fungi inoculation utensil, the adjustable inoculating gun of a kind of specifically edible fungi liquid strain continuous and quantitative.
Background technology
China's Edible Fungi mode has two kinds, and a kind of is the agricultural cultivation method, and its principal character is that bacterial classification all presents solid state from producing the cultivation process, and the shortcoming of Sheng Chaning is that labour intensity is big in this way, the expense height, the cycle is long, and it is slow to send out bacterium speed, cell age is inconsistent, waste grain, output instability; Another kind method is a solution fermentation, claims deep liquid culture technique (submerged fermentation technology) again, belongs to one of modern biotechnology, and is the same with the solid fermentation process, be with purebred microbial inoculant in the sterilising medium that contains the proper nutrition composition.
The application of liquid spawn in edible fungus culturing can be rated as the revolution of Edible Fungi, and it is representing the developing direction of Edible Fungi technology, and the popularization of liquid spawn will further promote the development of mushroom industry, this is that technology is easier because liquid spawn is compared with solid spawn, and bacterial classification is purer, cycle is shorter, and cost is low, and is easy and simple to handle, the cell age unanimity, fruiting is neat, is convenient to management, biology efficient is higher, spawn activity is strong, and it is fast to send out bacterium speed, characteristics such as suitable extensive entire-year production.
In order to adapt to China's Edible Fungi reality, adopt solution fermentation to carry out extensive bacterial classification production, will create prerequisite for the automation of Edible Fungi.As a whole, replacing the solid original seed to produce the solid state cultivation kind with liquid spawn, is practicable; And directly carry out artificial cultivation with liquid spawn, be tending towards perfect at present just gradually.Because liquid spawn is aging easily under normal temperature condition, not durable storage, production technology height, inoculation is strict, deferred assets are big, has limited its application in middle-size and small-size Edible Fungi unit.
Summary of the invention
The objective of the invention is to plant mode at the edible mushroom bag that China generally adopts at present, on traditional liquid-spawn inoculation method basis, but provide a kind of continuous inoculation easy and simple to handle, move and connect that bacterial classification can quantitatively be regulated, transfer needle is replaceable, speed is fast, connect the high adjustable inoculating gun of edible mushroom continuous and quantitative of bacterium success rate.
The present invention includes hollow housing, be positioned at the piston of housing, the handle that links to each other with housing, have through hole in the piston, piston nose is provided with seal groove, and sealing ring is housed in the seal groove, piston rear end is equipped with bacterium liquid inlet device, and piston nose is equipped with bacterium liquid outlet device.
Described bacterium liquid inlet device comprises first spring, adjusting nut, pressure rings, connecting rod, bearing pin, spanner, first valve core case, the import check valve body, first spool, second spring, piston rear end is with first spring outward, the housing afterbody is provided with adjusting nut, the front end of first spring contacts with the afterbody of housing, spring rear end contacts with adjusting nut, be with pressure rings on the adjusting nut, pressure rings links to each other with spanner by connecting rod, spanner is fixed on the spanner support by bearing pin, piston tail is connected with first valve core case by sealing gasket, first valve core case is connected with the import check valve body, the O RunddichtringO is installed in the import check valve body and the first valve core case contact-making surface, be provided with first spool in the import check valve body and first valve core case, first valve core upper sleeve has second spring.
Described bacterium liquid outlet device comprises the outlet check valve body, second valve core case, second spool, the 3rd spring, unidirectional outlet valve interface, the inoculation syringe needle, front end at housing connects the outlet check valve body by sealing gasket, be connected with second valve core case on the outlet check valve body, in the contact-making surface of the outlet check valve body and second valve core case, sealing ring is installed, in the outlet check valve body and second valve core case, be connected with second spool, second valve core upper sleeve has the 3rd spring, on second valve core case, be connected with unidirectional outlet valve interface, be connected with the inoculation syringe needle on the unidirectional outlet valve interface by sealing gasket.
Described piston rear end has screw thread, and the piston tail side is provided with scale.
The present invention is a kind of inoculating tool that is used to shift edible fungi liquid strain, can be widely used in the mushroom industry.It mainly is by piston moving axially in plunger shaft, liquid spawn is drawn and discharged to the volume that changes plunger shaft, when making the piston cavity space, moving axially of piston increase, the plunger shaft internal pressure reduces, because the action of pressure of external atmosphere pressure and cavity pressure, unidirectional import valve body by being attached thereto makes liquid spawn enter piston cavity; Reduce when moving axially of piston makes the piston cavity space, when the plunger shaft internal pressure increased, liquid spawn was discharged by the outlet check valve body that is attached thereto, and injected in the composts or fertilisers of cultivating through the inoculation syringe needle, finished the transfer of liquid spawn.Because the volume-variation in the plunger shaft is controlled by the distance that piston moves in the housing chamber, therefore the distance that moves by an adjusting nut control piston just can reach the purpose that the control liquid spawn shifts.
Beneficial effect of the present invention is:
1), design, the production of new inoculating tool.Cultivate for edible fungus liquid, aseptic technique is required height, and the easiest pollution of seeded process.In order to reach quick, pollution-free, to send out the fast purpose of bacterium, design, produce new practicality, cheapness, inoculating tool efficiently.
2), broken away from traditional connecing the bacterium chamber and connect bacterium case operating environment fully, greatly alleviated hand labor intensity, can under multiple occasion, use, flexible, adopt mechanization to replace each link of manual operations, have continuous inoculation, inoculum concentration is adjustable, and is easy and simple to handle, speed is fast, the efficient advantages of higher.
3), realize the supporting of liquid spawn and industrial cultivation technique, the application of edible fungi liquid strain and factory culture are the inexorable trends that edible mushroom is developed, both drive mutually, help the upgrading of mushroom industry.
4), can integral body carry out high pressure steam sterilization.
The present invention has easy and simple to handle, inoculation speed is fast, inoculum concentration can quantitatively be regulated, pollution-free, the efficient height significantly reduces hand labor intensity, the realization high efficiency is produced, and can integral body carry out advantages such as high pressure steam sterilization, and can satisfy batch production and produce use, also being fit to the family simultaneously is the workshop-based production use of unit.
Description of drawings
Fig. 1 is a structural representation of the present invention.
Embodiment
Following embodiment can better understand the present invention, but does not limit the present invention in any way.
The hollow circular cylinder handle 14 that removably is fixed together that the present invention includes hollow housing 1, is positioned at the piston 2 of housing 1, links to each other with housing 1, have manhole 22 in the piston 2, piston 2 front ends are provided with seal groove 23, in the seal groove 23 viton seal ring is housed, piston carries out dismountable the connection with housing, bacterium liquid inlet device is equipped with in piston 2 rear ends, and piston 2 front ends are equipped with bacterium liquid outlet device.
Bacterium liquid inlet device comprises first spring 3, adjusting nut 4, pressure rings 5, connecting rod 12, bearing pin 13, spanner 15, first valve core case 6, import check valve body 10, first spool 9, second spring 8, be with first spring 3 outside piston 2 rear ends, housing 1 afterbody is provided with adjusting nut 4, the front end of first spring 3 contacts with the afterbody of housing 1, restoring force is provided, spring rear end contacts with adjusting nut 4, be used to regulate the bacterial classification flow, be with pressure rings 5 on the adjusting nut 4, pressure rings 5 links to each other with spanner 15 by connecting rod 12, spanner 15 is fixed on the spanner support 26 by bearing pin 13, piston 2 afterbodys removably are connected with first valve core case 6 by sealing gasket 11, removably be connected with import check valve body 10 at first valve core case 6, O RunddichtringO 7 is installed in import check valve body 10 and first valve core case, 6 contact-making surfaces, and the another port of import check valve body 10 links to each other with the liquid spawn container by flexible pipe.In the import check valve body 10 and first valve core case 6, be provided with on first spool, 9, the first spools 9 and removably be with second spring 8.
Bacterium liquid outlet device comprises outlet check valve body 16, second valve core case 18, second spool 20, the 3rd spring 19, unidirectional outlet valve interface 17, inoculation syringe needle 21, front end at housing 1 removably connects outlet check valve body 16 by sealing gasket, removably be connected with second valve core case 18 on the outlet check valve body 16, in the contact-making surface of the outlet check valve body 16 and second valve core case 18, sealing ring is installed, in the outlet check valve body 16 and second valve core case 18, removably be connected with second spool 20, removably be with the 3rd spring 19 on second spool 20, on second valve core case 18, removably be connected with unidirectional outlet valve interface 17, removably be connected with inoculation syringe needle 21 on the unidirectional outlet valve interface 17 by sealing gasket.
There is screw thread piston 2 rear ends, and piston 2 afterbody sides are provided with scale 24.Can be used for quantitatively connecing the bacterium amount.
Operation principle of the present invention and using method are as follows:
When under normal circumstances inoculating, earlier adjusting nut 4 is adjusted to needed volume, hand-held inoculating gun is inwardly pulled spanner 15, spanner is at the effect lower edge of grip support motion, active force is passed to pressure rings 5 and finally by adjusting nut power passed to piston 2 by connecting rod 12, drive piston advances forward and oppress the returning spring compression simultaneously.After loosing one's grip, the restoring force of spring drives piston by adjusting nut 4 and pressure rings is moved backward, and restoring force is delivered to spanner by pressure rings by connecting rod again resets spanner.Piston has been finished in housing once and has been moved back and forth like this, is constantly pulling and is loosening in the spanner process, and piston will constantly move back and forth.
During work, the handle of holding with a firm grip is pulled spanner, piston advances forward, and the space diminishes in the housing, and pressure raises, and under pressure, the outlet check valve is opened, and inlet one-way valve is closed, and the bacterial classification medium is flowed out by the outlet check valve under the effect of pressure; When loosening spanner, piston moves backward, and the space becomes big in the housing, pressure reduces, the outlet closed check valve, outside bacterial classification medium is delivered to atmospheric pressure the import check valve and opens the import check valve, and outside bacterial classification medium replenishes into shell space under the effect of atmospheric pressure.
The present invention has broken away from traditional connecing the bacterium chamber and connect bacterium case operating environment fully, has greatly alleviated hand labor intensity, can use under multiple occasion.

Claims (2)

1. adjustable inoculating gun of edible mushroom continuous and quantitative, it is characterized in that: the handle (14) that it comprises hollow housing (1), is positioned at the piston (2) of housing (1), links to each other with housing (1), have through hole (22) in the piston (2), piston (2) front end is provided with seal groove (23), seal groove is equipped with sealing ring in (23), bacterium liquid inlet device is equipped with in piston (2) rear end, and piston (2) front end is equipped with bacterium liquid outlet device; Described bacterium liquid inlet device comprises first spring (3), adjusting nut (4), pressure rings (5), connecting rod (12), bearing pin (13), spanner (15), first valve core case (6), import check valve body (10), first spool (9), second spring (8), piston (2) is with first spring (3) outside the rear end, housing (1) afterbody is provided with adjusting nut (4), the front end of first spring (3) contacts with the afterbody of housing (1), spring rear end contacts with adjusting nut (4), be with pressure rings (5) on the adjusting nut (4), pressure rings (5) links to each other with spanner (15) by connecting rod (12), spanner (15) is fixed on the spanner support (26) by bearing pin (13), piston (2) afterbody is connected with first valve core case (6) by sealing gasket (11), first valve core case (6) is connected with import check valve body (10), O RunddichtringO (7) is installed in import check valve body (10) and first valve core case (6) contact-making surface, in import check valve body (10) and first valve core case (6), be provided with first spool (9), be with second spring (8) on first spool (9); Described bacterium liquid outlet device comprises outlet check valve body (16), second valve core case (18), second spool (20), the 3rd spring (19), unidirectional outlet valve interface (17), inoculation syringe needle (21), front end at housing (1) connects outlet check valve body (16) by sealing gasket, be connected with second valve core case (18) on the outlet check valve body (16), sealing ring is installed in the contact-making surface of outlet check valve body (16) and second valve core case (18), in outlet check valve body (16) and second valve core case (18), be connected with second spool (20), be with the 3rd spring (19) on second spool (20), upward be connected with unidirectional outlet valve interface (17) at second valve core case (18), be connected with inoculation syringe needle (21) on the unidirectional outlet valve interface (17) by sealing gasket.
2. the adjustable inoculating gun of edible mushroom continuous and quantitative according to claim 1 is characterized in that: there is screw thread described piston (2) rear end, and piston (2) afterbody side is provided with scale (24).
CN200910117351XA 2009-07-04 2009-07-04 Continuous quantitative adjustable inoculation gun for edible fungi Expired - Fee Related CN101940124B (en)

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CN101940124B true CN101940124B (en) 2011-08-10

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Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102550291A (en) * 2012-01-18 2012-07-11 山西强盛种业有限公司 Edible fungus liquid strain inoculation and injection gun
CN103103119B (en) * 2013-01-29 2014-07-02 常熟南师大发展研究院有限公司 Continuous quantitative microbial inoculation device
CN103704015B (en) * 2013-12-17 2015-09-09 福建农林大学 A kind of special inoculating gun of Cordceps militaris bacterial classification and making and use method thereof
CN106954463B (en) * 2017-04-24 2022-09-30 山东省蚕业研究所 Silkworm cordyceps inoculation device and application
CN113186081A (en) * 2021-05-22 2021-07-30 甘肃农业大学 Adjustable fungus device that connects of concentration

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2104539U (en) * 1991-10-15 1992-05-20 张从明 Jaw inoculator for solide edible mushroom vaccine
CN1066467A (en) * 1991-05-04 1992-11-25 吴锦扬 Inoculating gun for edible fungus in bags
CN2651256Y (en) * 2003-08-15 2004-10-27 连云港国鑫医药设备有限公司 Liquid culture transfer apparatus
CN2706475Y (en) * 2004-05-18 2005-06-29 沈阳大学 Ozone ion gun type inoculator
CN1800339A (en) * 2005-09-29 2006-07-12 江苏大学 Integral device for inoculating and culturing for edible mushroom liquid strain
CN201138955Y (en) * 2007-12-31 2008-10-29 郭宏旗 Glossy ganoderma mushroom deep layer positioning beads inoculator
JP4278026B2 (en) * 2002-08-27 2009-06-10 株式会社スリオンテック Flame-retardant adhesive tape and method for producing the same
JP4356135B2 (en) * 1998-04-20 2009-11-04 ソニー株式会社 Color display device
CN201550451U (en) * 2009-07-04 2010-08-18 甘肃省科学院生物研究所 Continuous quantitative adjustable edible fungi inoculating gun

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1066467A (en) * 1991-05-04 1992-11-25 吴锦扬 Inoculating gun for edible fungus in bags
CN2104539U (en) * 1991-10-15 1992-05-20 张从明 Jaw inoculator for solide edible mushroom vaccine
JP4356135B2 (en) * 1998-04-20 2009-11-04 ソニー株式会社 Color display device
JP4278026B2 (en) * 2002-08-27 2009-06-10 株式会社スリオンテック Flame-retardant adhesive tape and method for producing the same
CN2651256Y (en) * 2003-08-15 2004-10-27 连云港国鑫医药设备有限公司 Liquid culture transfer apparatus
CN2706475Y (en) * 2004-05-18 2005-06-29 沈阳大学 Ozone ion gun type inoculator
CN1800339A (en) * 2005-09-29 2006-07-12 江苏大学 Integral device for inoculating and culturing for edible mushroom liquid strain
CN201138955Y (en) * 2007-12-31 2008-10-29 郭宏旗 Glossy ganoderma mushroom deep layer positioning beads inoculator
CN201550451U (en) * 2009-07-04 2010-08-18 甘肃省科学院生物研究所 Continuous quantitative adjustable edible fungi inoculating gun

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