CN105199993A - Photosynthetic bacteria culture medium and preparation method thereof - Google Patents
Photosynthetic bacteria culture medium and preparation method thereof Download PDFInfo
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- 239000001963 growth medium Substances 0.000 title claims abstract description 61
- 241000894006 Bacteria Species 0.000 title claims abstract description 58
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 53
- 238000002360 preparation method Methods 0.000 title claims abstract description 12
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000002609 medium Substances 0.000 claims abstract description 18
- BDKLKNJTMLIAFE-UHFFFAOYSA-N 2-(3-fluorophenyl)-1,3-oxazole-4-carbaldehyde Chemical compound FC1=CC=CC(C=2OC=C(C=O)N=2)=C1 BDKLKNJTMLIAFE-UHFFFAOYSA-N 0.000 claims abstract description 15
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims abstract description 15
- 235000011130 ammonium sulphate Nutrition 0.000 claims abstract description 15
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims abstract description 15
- 235000019341 magnesium sulphate Nutrition 0.000 claims abstract description 15
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims abstract description 15
- 235000019796 monopotassium phosphate Nutrition 0.000 claims abstract description 15
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 15
- 229940087562 sodium acetate trihydrate Drugs 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 12
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims abstract description 11
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 12
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 7
- 229940041514 candida albicans extract Drugs 0.000 abstract description 3
- 239000011550 stock solution Substances 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 239000012138 yeast extract Substances 0.000 abstract description 3
- 150000001875 compounds Chemical class 0.000 abstract description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 8
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 6
- 241000190932 Rhodopseudomonas Species 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 239000000306 component Substances 0.000 description 4
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 235000017557 sodium bicarbonate Nutrition 0.000 description 4
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 4
- 241000190967 Rhodospirillum Species 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000012533 medium component Substances 0.000 description 2
- 230000029553 photosynthesis Effects 0.000 description 2
- 238000010672 photosynthesis Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- 239000001301 oxygen Substances 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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- 239000010865 sewage Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
本发明公开了一种光合细菌培养基及其制备方法。每L所述光合细菌培养基的组成为：三水合乙酸钠4～10g，硫酸铵1～5g，碳酸氢钠1～5g，磷酸二氢钾0.2～0.8g，硫酸镁0.2～0.8g，以及余量的水。所述光合细菌培养基的pH值为5.5～8.5。配制所述培养基时，将所述组分溶解于水中，然后调配pH值后定容即可。本发明提供的光合细菌培养基，其组成简单，仅由常规的5种化合物组成，未选用现有的培养基中的酵母膏和T.M储液等高价格物质，因此降低了生产成本，如与RCVBN培养基相比，产出1千克菌体培养基的费用能节省最高达85％；本发明的配制工艺简单易行，可操作性强；采用本发明培养基培养，光合细菌浓度高，OD660高达2.2。The invention discloses a photosynthetic bacteria culture medium and a preparation method thereof. The composition of the photosynthetic bacteria medium per L is: 4-10 g of sodium acetate trihydrate, 1-5 g of ammonium sulfate, 1-5 g of sodium bicarbonate, 0.2-0.8 g of potassium dihydrogen phosphate, 0.2-0.8 g of magnesium sulfate, and remaining water. The pH value of the photosynthetic bacteria culture medium is 5.5-8.5. When preparing the culture medium, the components are dissolved in water, and then the pH value is adjusted and the volume is constant. The photosynthetic bacteria culture medium provided by the invention has simple composition, only consists of 5 conventional compounds, does not select high-priced substances such as yeast extract and TM stock solution in the existing culture medium, thus reducing production costs, such as with Compared with RCVBN culture medium, the cost of producing 1 kilogram of bacterial culture medium can save up to 85%; the preparation process of the present invention is simple and easy, and the operability is strong; the culture medium of the present invention has high concentration of photosynthetic bacteria and OD 660 up to 2.2.
Description
技术领域technical field
本发明涉及一种细菌培养基及其制备方法，具体涉及一种光合细菌培养基及其制备方法。The invention relates to a bacterial culture medium and a preparation method thereof, in particular to a photosynthetic bacteria culture medium and a preparation method thereof.
背景技术Background technique
光合细菌是一类能够进行光合作用而不产氧的特殊微生物群，广泛分布于海洋、河流、湖泊和土壤中，能在厌氧光照或好氧黑暗条件下利用自然界中的有机物、硫化物、氨等作为供氢体兼碳源进行光合作用，光合细菌富含蛋白质、生物高聚物、抗生素、类胡萝卜素、泛酸以及一些药用物质，现已广泛用于养殖业，种植业、污水处理等。基于以上优势，本应加大光合细菌推广力度，但由于用于培养光合细菌的培养基具有平均富集时间较长、培养基成分种类多以及成本较高等不足，限制了光合细菌在生产上的广泛应用。Photosynthetic bacteria are a special group of microorganisms that can carry out photosynthesis without producing oxygen. They are widely distributed in oceans, rivers, lakes and soils, and can utilize organic matter, sulfide, Ammonia, etc. are used as hydrogen donors and carbon sources for photosynthesis. Photosynthetic bacteria are rich in proteins, biopolymers, antibiotics, carotenoids, pantothenic acid, and some medicinal substances. They are now widely used in aquaculture, planting, and sewage treatment wait. Based on the above advantages, the promotion of photosynthetic bacteria should be increased. However, the medium used for cultivating photosynthetic bacteria has the disadvantages of long average enrichment time, various types of medium components, and high cost, which limits the use of photosynthetic bacteria in production. widely used.
根据研究现状表明，传统RCVBN培养基成分种类多，配制工艺复杂，光合细菌培养周期为3～5天，因此关于光合细菌培养基的研究逐渐受到重视，已有多位学者对其进行了优化，通过对培养基优化，减少了用量，提高了光合细菌的产量，如张伟研究出的光合细菌推荐培养基，操玉涛等研究出的改良71#培养基，刘军义等研究出的光合细菌计数培养基，刘昆等对光合细菌成产配方的优化研究等。但以上培养基成分种类仍较多，其中含有酵母膏和T.M储液等，生产成本高，不利于光合细菌的大规模工业化成产，因此亟需提供一种组成成分简单、成本低以及培养周期短的培养基。According to the research status, the traditional RCVBN culture medium has many kinds of ingredients, complicated preparation process, and the photosynthetic bacteria culture period is 3 to 5 days. Therefore, the research on the photosynthetic bacteria culture medium has gradually received attention, and many scholars have optimized it. By optimizing the culture medium, the amount of photosynthetic bacteria is reduced and the yield of photosynthetic bacteria is increased. For example, the recommended medium for photosynthetic bacteria developed by Zhang Wei, the improved 71# medium developed by Cao Yutao et al., and the count of photosynthetic bacteria developed by Liu Junyi et al. Culture medium, Liu Kun et al.'s optimization research on the production formula of photosynthetic bacteria, etc. However, there are still many kinds of medium components above, including yeast extract and T.M stock solution, etc., and the production cost is high, which is not conducive to the large-scale industrial production of photosynthetic bacteria. short medium.
发明内容Contents of the invention
本发明的目的是提供一种组成简单的光合细菌培养基，解决了现有光合细菌培养基存在现有光合细菌培养基平均富集时间较长、培养基成分种类多以及成本较高等技术问题。The purpose of the present invention is to provide a photosynthetic bacteria culture medium with simple composition, which solves the technical problems of the existing photosynthetic bacteria culture medium such as long average enrichment time, many types of culture medium components and high cost.
本发明所提供的光合细菌培养基，其组成为：The photosynthetic bacteria culture medium provided by the present invention consists of:
每L所述光合细菌培养基的组成为：The composition of the photosynthetic bacteria culture medium described in every L is:
三水合乙酸钠4～10g，硫酸铵1～5g，碳酸氢钠1～5g，磷酸二氢钾0.2～0.8g，硫酸镁0.2～0.8g，以及余量的水。4-10 g of sodium acetate trihydrate, 1-5 g of ammonium sulfate, 1-5 g of sodium bicarbonate, 0.2-0.8 g of potassium dihydrogen phosphate, 0.2-0.8 g of magnesium sulfate, and the rest of water.
进一步地，每L所述光合细菌培养基的组成可为：Further, the composition of the photosynthetic bacteria medium per L can be:
三水合乙酸钠6～10g，硫酸铵2～5g，碳酸氢钠2～5g，磷酸二氢钾0.2～0.8g，硫酸镁0.2～0.8g，以及余量的水。6-10 g of sodium acetate trihydrate, 2-5 g of ammonium sulfate, 2-5 g of sodium bicarbonate, 0.2-0.8 g of potassium dihydrogen phosphate, 0.2-0.8 g of magnesium sulfate, and the rest of water.
上述的光合细菌培养基的pH值可为5.5～8.5，具体可为7.5。The pH value of the above-mentioned photosynthetic bacteria culture medium may be 5.5-8.5, specifically 7.5.
上述的光合细菌培养基的具体组成为：The concrete composition of above-mentioned photosynthetic bacteria culture medium is:
每L所述光合细菌培养基的组成为：三水合乙酸钠6g，硫酸铵2g，碳酸氢钠2g，磷酸二氢钾0.4g，硫酸镁0.4g，以及余量的水；或，The composition of the photosynthetic bacteria culture medium per L is: sodium acetate trihydrate 6g, ammonium sulfate 2g, sodium bicarbonate 2g, potassium dihydrogen phosphate 0.4g, magnesium sulfate 0.4g, and the water of balance; Or,
每L所述光合细菌培养基的组成为：三水合乙酸钠10g，硫酸铵5g，碳酸氢钠5g，磷酸二氢钾0.8g，硫酸镁0.8g，以及余量的水。The composition of the photosynthetic bacteria culture medium per L is: sodium acetate trihydrate 10g, ammonium sulfate 5g, sodium bicarbonate 5g, potassium dihydrogen phosphate 0.8g, magnesium sulfate 0.8g, and the water of balance.
本发明光合细菌培养基可通过如下方法进行制备：Photosynthetic bacteria culture medium of the present invention can be prepared by following method:
将所述三水合乙酸钠、所述硫酸铵、所述碳酸氢钠、所述磷酸二氢钾和所述硫酸镁溶解于水中，经定容后即得所述培养基。The sodium acetate trihydrate, the ammonium sulfate, the sodium bicarbonate, the potassium dihydrogen phosphate and the magnesium sulfate are dissolved in water, and the culture medium is obtained after constant volume.
上述的制备方法中，还包括对所述培养基进行pH值调控的步骤。In the above preparation method, the step of adjusting the pH value of the culture medium is also included.
上述的制备方法中，利用氢氧化钠和盐酸调控所述培养基的pH值。In the above preparation method, sodium hydroxide and hydrochloric acid are used to regulate the pH value of the medium.
本发明提供的培养基可用于培养细菌，如光合细菌。The culture medium provided by the invention can be used for culturing bacteria, such as photosynthetic bacteria.
本发明提供的光合细菌培养基，其组成简单，仅由常规的5种化合物组成，未选用现有的培养基中的酵母膏和T.M储液等高价格物质，因此降低了生产成本，如与RCVBN培养基相比，产出1千克菌体培养基的费用能节省最高达85％；本发明的配制工艺简单易行，可操作性强；采用本发明培养基培养，光合细菌浓度高，OD660高达2.2。The photosynthetic bacteria culture medium provided by the invention has simple composition, only consists of 5 conventional compounds, does not select high-priced substances such as yeast extract and TM stock solution in the existing culture medium, thus reducing production costs, such as with Compared with RCVBN culture medium, the cost of producing 1 kilogram of bacterial culture medium can save up to 85%; the preparation process of the present invention is simple and easy, and the operability is strong; the culture medium of the present invention has high concentration of photosynthetic bacteria and OD 660 up to 2.2.
附图说明Description of drawings
图1为本发明实施例1培养基培养光合细菌时的菌体生长情况。Fig. 1 is the thalline growth situation when photosynthetic bacteria are cultivated in the culture medium of Example 1 of the present invention.
图2为本发明实施例2培养基培养光合细菌时的菌体生长情况。Fig. 2 is the thalline growth situation when photosynthetic bacteria are cultured in the culture medium of Example 2 of the present invention.
具体实施方式Detailed ways
下述实施例中所使用的实验方法如无特殊说明，均为常规方法。The experimental methods used in the following examples are conventional methods unless otherwise specified.
下述实施例中所用的材料、试剂等，如无特殊说明，均可从商业途径得到。The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
实施例1、制备光合细菌培养基Embodiment 1, prepare photosynthetic bacteria culture medium
称取如下组分：6g三水合乙酸钠、2g硫酸铵、2g碳酸氢钠、0.4g磷酸二氢钾、0.4g硫酸镁。Weigh the following components: 6g sodium acetate trihydrate, 2g ammonium sulfate, 2g sodium bicarbonate, 0.4g potassium dihydrogen phosphate, 0.4g magnesium sulfate.
按照如下方法进行配制：Prepare as follows:
量取约500ml去离子水，准确称量6g三水合乙酸钠加入水中溶解，准确称量2g硫酸铵加入水中溶解，准确称量2g碳酸氢钠加入水中溶解，准确称量0.4g磷酸二氢钾加入水中溶解，准确称量0.4g硫酸镁加入水中溶解。然后向溶液中补充去离子水至约990ml，搅拌均匀。使用氢氧化钠及盐酸调节溶液pH至7.5。最后将溶液定容至1L。Measure about 500ml of deionized water, accurately weigh 6g of sodium acetate trihydrate and add to water to dissolve, accurately weigh 2g of ammonium sulfate and add to water to dissolve, accurately weigh 2g of sodium bicarbonate and add to water to dissolve, accurately weigh 0.4g of potassium dihydrogen phosphate Add water to dissolve, accurately weigh 0.4g magnesium sulfate and add to water to dissolve. Then add deionized water to the solution to about 990ml, and stir evenly. The pH of the solution was adjusted to 7.5 using sodium hydroxide and hydrochloric acid. Finally, the solution was adjusted to 1 L.
本发明培养基与RCVBN培养基的成本比较，如表1和表2所示：The cost comparison of culture medium of the present invention and RCVBN culture medium, as shown in table 1 and table 2:
表1本发明实施例1培养基的成本Table 1 The cost of embodiment 1 culture medium of the present invention
表2RCVBN培养基的成本Table 2 Cost of RCVBN medium
对比表1和表2中的数据可以得出，本发明培养基显著降低了生产成本；与RCVBN培养基相比，产出1千克菌体培养基的费用能节省85％。Comparing the data in Table 1 and Table 2, it can be concluded that the culture medium of the present invention significantly reduces the production cost; compared with the RCVBN culture medium, the cost of producing 1 kg of bacterial culture medium can save 85%.
利用上述制备的培养基进行光合细菌的培养：Utilize the medium prepared above to carry out the cultivation of photosynthetic bacteria:
取一定体积配制好的培养基于锥形瓶中，按照30％光合细菌成品菌液(其中光合细菌为红螺菌、红假单胞菌、外硫沼泽红假单胞菌；购自北京渔经生物技术有限责任公司)：70％培养基(体积比)接种。。接种后使用透氧封口膜将锥形瓶封口，置于60W白炽灯光下静置或摇床振荡培养，光照强度控制在3000lux，温度控制在26～30℃。培养2～3天即能得到成品菌。Take a certain volume of prepared culture based on Erlenmeyer flask, according to 30% photosynthetic bacteria finished bacterial liquid (wherein the photosynthetic bacteria are Rhodospirillum, Rhodopseudomonas, and Rhodopseudomonas exosulfurs; purchased from Beijing Yujing Biotechnology Co., Ltd.): 70% medium (volume ratio) inoculation. . After inoculation, seal the Erlenmeyer flask with an oxygen-permeable sealing film, and place it under a 60W incandescent light to stand still or shake it on a shaker for cultivation. The light intensity is controlled at 3000lux, and the temperature is controlled at 26-30°C. The finished bacteria can be obtained after culturing for 2 to 3 days.
上述培养过程中，菌体生长情况如图1所示，由图1可以看出，本发明培养基培养的光合细菌的浓度高，OD660高达2.1，达到甚至超过RCVBN培养基所得的菌体浓度(为1.8左右，其培养方案与本发明培养基的培养方案相同)。In the above-mentioned cultivation process, the thalline growth situation is as shown in Figure 1, as can be seen from Figure 1, the concentration of photosynthetic bacteria cultivated in the medium of the present invention is high, and OD660 is up to 2.1, reaching or even surpassing the thalline concentration of RCVBN medium gained (being about 1.8, its culture scheme is identical with the culture scheme of culture medium of the present invention).
实施例2、制备光合细菌培养基Embodiment 2, prepare photosynthetic bacteria culture medium
称取如下组分：称取如下组分：10g三水合乙酸钠、5g硫酸铵、5g碳酸氢钠、0.8g磷酸二氢钾、0.8g硫酸镁。Weigh the following components: Weigh the following components: 10g sodium acetate trihydrate, 5g ammonium sulfate, 5g sodium bicarbonate, 0.8g potassium dihydrogen phosphate, 0.8g magnesium sulfate.
按照如下方法进行配制：Prepare as follows:
量取约500ml去离子水，准确称量10g三水合乙酸钠加入水中溶解，准确称量5g硫酸铵加入水中溶解，准确称量5g碳酸氢钠加入水中溶解，准确称量0.8g磷酸二氢钾加入水中溶解，准确称量0.8g硫酸镁加入水中溶解。然后向溶液中补充去离子水至约990ml，搅拌均匀。使用氢氧化钠及盐酸调节溶液pH至7.5。最后将溶液定容至1L。Measure about 500ml of deionized water, accurately weigh 10g of sodium acetate trihydrate and add to water to dissolve, accurately weigh 5g of ammonium sulfate and add to water to dissolve, accurately weigh 5g of sodium bicarbonate and add to water to dissolve, accurately weigh 0.8g of potassium dihydrogen phosphate Add water to dissolve, accurately weigh 0.8g of magnesium sulfate and add to water to dissolve. Then add deionized water to the solution to about 990ml, and stir evenly. The pH of the solution was adjusted to 7.5 using sodium hydroxide and hydrochloric acid. Finally, the solution was adjusted to 1 L.
本发明培养基与RCVBN培养基的成本比较，如表3和表2所示：The cost comparison of substratum of the present invention and RCVBN substratum, as shown in table 3 and table 2:
表3本发明实施例2培养基的成本Table 3 The cost of embodiment 2 culture medium of the present invention
对比表3和表2中的数据可以得出，本发明培养基显著降低了生产成本；与RCVBN培养基相比，产出1千克菌体培养基的费用能节省约70％。Comparing the data in Table 3 and Table 2, it can be concluded that the culture medium of the present invention significantly reduces the production cost; compared with the RCVBN culture medium, the cost of producing 1 kg of bacterial culture medium can save about 70%.
利用上述制备的培养基进行光合细菌的培养：Utilize the medium prepared above to carry out the cultivation of photosynthetic bacteria:
取一定体积配制好的培养基于锥形瓶中，按照30％光合细菌成品菌液(其中光合细菌为红螺菌、红假单胞菌、外硫沼泽红假单胞菌；购自北京渔经生物技术有限责任公司)：70％培养基(体积比)接种。其中光合细菌为红螺菌、红假单胞菌、外硫沼泽红假单胞菌。接种后使用透氧封口膜将锥形瓶封口，置于60W白炽灯光下静置或摇床振荡培养，光照强度控制在3000lux，温度控制在26～30℃。培养2～3天即能得到成品菌。Take a certain volume of prepared culture based on Erlenmeyer flask, according to 30% photosynthetic bacteria finished bacterial liquid (wherein the photosynthetic bacteria are Rhodospirillum, Rhodopseudomonas, Exosulfuropseudomonas Rhodopseudomonas; purchased from Beijing Yujing Biotechnology Co., Ltd.): 70% medium (volume ratio) inoculation. The photosynthetic bacteria are Rhodospirillum, Rhodopseudomonas, and Rhodopseudomonas exosulfur. After inoculation, seal the Erlenmeyer flask with an oxygen-permeable sealing film, and place it under a 60W incandescent light to stand still or shake it on a shaker for cultivation. The light intensity is controlled at 3000lux, and the temperature is controlled at 26-30°C. The finished bacteria can be obtained after culturing for 2 to 3 days.
上述培养过程中，菌体生长情况如图2所示，由图2可以看出，本发明培养基培养的光合细菌的浓度高，OD660高达2.2，达到甚至超过RCVBN培养基所得的菌体浓度(为1.8左右，其培养方案与本发明培养基的培养方案相同)。In the above-mentioned cultivation process, the thalline growth situation is as shown in Figure 2, as can be seen from Figure 2, the concentration of photosynthetic bacteria cultivated in the medium of the present invention is high, and OD660 is up to 2.2, reaching or even surpassing the thalline concentration of RCVBN medium gained (being about 1.8, its culture scheme is identical with the culture scheme of culture medium of the present invention).
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543146A (en) * | 2016-02-02 | 2016-05-04 | 田维熙 | Preparation method of photosynthetic bacterium water aqua and special culture medium of photosynthetic bacterium water aqua |
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN109810915A (en) * | 2018-04-19 | 2019-05-28 | 湛江恒兴养殖技术服务有限公司 | A kind of photosynthetic bacteria culture simplicity spreads cultivation culture medium and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706933A (en) * | 2004-06-11 | 2005-12-14 | 郭伟光 | Prepn process of high-concentration photosynthesis bacterial prepn |
| CN101113418A (en) * | 2007-07-06 | 2008-01-30 | 辽宁大学 | A medium for enriching photosynthetic bacteria |
| CN102911868A (en) * | 2012-09-28 | 2013-02-06 | 新奥科技发展有限公司 | Microorganism culture medium and microorganism culture method |
-
2015
- 2015-10-22 CN CN201510691293.7A patent/CN105199993A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706933A (en) * | 2004-06-11 | 2005-12-14 | 郭伟光 | Prepn process of high-concentration photosynthesis bacterial prepn |
| CN101113418A (en) * | 2007-07-06 | 2008-01-30 | 辽宁大学 | A medium for enriching photosynthetic bacteria |
| CN102911868A (en) * | 2012-09-28 | 2013-02-06 | 新奥科技发展有限公司 | Microorganism culture medium and microorganism culture method |
Non-Patent Citations (3)
| Title |
|---|
| 张红莲: "富集光合细菌培养基的优化研究", 《水产科学》 * |
| 李亚丽: "光合细菌菌落特性的研究现状", 《农业技术与装备》 * |
| 洪坚平等: "《应用微生物学》", 28 February 2011 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543146A (en) * | 2016-02-02 | 2016-05-04 | 田维熙 | Preparation method of photosynthetic bacterium water aqua and special culture medium of photosynthetic bacterium water aqua |
| CN105543146B (en) * | 2016-02-02 | 2018-11-13 | 田维熙 | A kind of preparation method and its special culture media of photosynthetic bacteria aqua |
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN108220203B (en) * | 2018-03-06 | 2021-03-02 | 上海海洋大学 | A kind of fermentation medium of Rhodobacter sphaeroides and its application in the fermentation production of Rhodobacter sphaeroides |
| CN109810915A (en) * | 2018-04-19 | 2019-05-28 | 湛江恒兴养殖技术服务有限公司 | A kind of photosynthetic bacteria culture simplicity spreads cultivation culture medium and preparation method thereof |
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