CN105199993A - Photosynthetic bacteria culture medium and preparation method thereof - Google Patents
Photosynthetic bacteria culture medium and preparation method thereof Download PDFInfo
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- CN105199993A CN105199993A CN201510691293.7A CN201510691293A CN105199993A CN 105199993 A CN105199993 A CN 105199993A CN 201510691293 A CN201510691293 A CN 201510691293A CN 105199993 A CN105199993 A CN 105199993A
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- culture medium
- photosynthetic bacteria
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Abstract
The invention discloses a photosynthetic bacteria culture medium and a preparation method thereof. Each liter of the photosynthetic bacteria culture medium is prepared from the following components: 4g to 10g of sodium acetate trihydrate, 1g to 5g of ammonium sulfate, 1g to 5g of sodium hydrogen carbonate, 0.2g to 0.8g of monopotassium phosphate, 0.2g to 0.8g of magnesium sulfate and the balance of water. The pH value of the photosynthetic bacteria culture medium is 5.5 to 8.5. When the culture medium is prepared, the components are dissolved into the water, the pH value is regulated and then the volume is made to be constant. The photosynthetic bacteria culture medium provided by the invention has simple components and is only prepared from five types of common compounds; high-price substances including yeast extract, T.M storage liquid and the like in an existing culture medium are not used so that the production cost is reduced; compared with an RCVBN culture medium, the cost for producing 1kg of a bacterium culture medium can be saved by a maximum of 85%; the preparation process is simple and feasible and the operability is strong; by utilizing the culture medium to culture, the concentration of photosynthetic bacteria is high and the content of OD660 is up to 2.2.
Description
Technical field
The present invention relates to a kind of bacteria culture medium and preparation method thereof, be specifically related to a kind of photosynthetic bacteria culture medium and preparation method thereof.
Background technology
Photosynthetic bacterium is the peculiar microorganism group that a class can carry out photosynthesis and non-oxygen-production, be distributed widely in ocean, river, lake and soil, the organism of occurring in nature, sulfide, ammonia etc. can be utilized under anaerobism illumination or aerobic dark condition to carry out photosynthesis as hydrogen donor carbon source of holding concurrently, photosynthetic bacterium rich in proteins, biopolymer, microbiotic, carotenoid, pantothenic acid and some medicinal substances, now be widely used in aquaculture, plant husbandry, sewage disposal etc.Based on above advantage, photosynthetic bacterium promotion efficiency should be strengthened, but due to the substratum for cultivating photosynthetic bacterium have that average enrichment time is longer, medium component kind mainly with and the more high deficiency of cost, limit the widespread use of photosynthetic bacterium on producing.
Show according to present Research, tradition RCVBN medium component kind is many, blending process is complicated, photosynthetic bacterium culture cycle is 3 ~ 5 days, therefore the research about photosynthetic bacteria culture medium comes into one's own gradually, existing multidigit scholar is optimized it, by to medium optimization, decrease consumption, improve the output of photosynthetic bacterium, the photosynthetic bacterium worked out as Zhang Wei recommends substratum, grasps the improvement 71# substratum that beautiful great waves etc. work out, the photosynthetic bacteria counting substratum that Liu Junyi etc. work out, Liu Kun etc. become to produce the optimizing research etc. of formula to photosynthetic bacterium.But above medium component kind is still more, wherein containing yeast extract paste and T.M liquid storage etc., production cost is high, is unfavorable for that the large-scale industry of photosynthetic bacterium changes into product, therefore needs badly and provides the substratum that a kind of moiety is simple, cost is low and culture cycle is short.
Summary of the invention
The object of this invention is to provide the simple photosynthetic bacteria culture medium of a kind of composition, solve existing photosynthetic bacteria culture medium and have that the average enrichment time of existing photosynthetic bacteria culture medium is longer, medium component kind mainly with and cost comparatively high-technology problem.
Photosynthetic bacteria culture medium provided by the present invention, it consists of:
Consisting of of photosynthetic bacteria culture medium described in every L:
Sodium acetate trihydrate 4 ~ 10g, ammonium sulfate 1 ~ 5g, sodium bicarbonate 1 ~ 5g, potassium primary phosphate 0.2 ~ 0.8g, magnesium sulfate 0.2 ~ 0.8g, and the water of surplus.
Further, described in every L, the composition of photosynthetic bacteria culture medium can be:
Sodium acetate trihydrate 6 ~ 10g, ammonium sulfate 2 ~ 5g, sodium bicarbonate 2 ~ 5g, potassium primary phosphate 0.2 ~ 0.8g, magnesium sulfate 0.2 ~ 0.8g, and the water of surplus.
The pH value of above-mentioned photosynthetic bacteria culture medium can be 5.5 ~ 8.5, specifically can be 7.5.
Specifically consisting of of above-mentioned photosynthetic bacteria culture medium:
Consisting of of photosynthetic bacteria culture medium described in every L: sodium acetate trihydrate 6g, ammonium sulfate 2g, sodium bicarbonate 2g, potassium primary phosphate 0.4g, magnesium sulfate 0.4g, and the water of surplus; Or,
Consisting of of photosynthetic bacteria culture medium described in every L: sodium acetate trihydrate 10g, ammonium sulfate 5g, sodium bicarbonate 5g, potassium primary phosphate 0.8g, magnesium sulfate 0.8g, and the water of surplus.
Photosynthetic bacteria culture medium of the present invention is prepared by following method:
Described sodium acetate trihydrate, described ammonium sulfate, described sodium bicarbonate, described potassium primary phosphate and described magnesium sulfate are dissolved in the water, after constant volume, namely obtain described substratum.
In above-mentioned preparation method, also comprise the step of described substratum being carried out to pH value regulation and control.
In above-mentioned preparation method, sodium hydroxide and hydrochloric acid is utilized to regulate and control the pH value of described substratum.
Substratum provided by the invention can be used for culturing bacterium, as photosynthetic bacterium.
Photosynthetic bacteria culture medium provided by the invention, its composition is simple, only be made up of 5 kinds of compounds of routine, do not select the high price material such as yeast extract paste and T.M liquid storage in existing substratum, because this reducing production cost, as compared with RCVBN substratum, the expense of output 1 kilogram of yeast culture base can be saved and is up to 85%; Blending process of the present invention is simple, workable; Adopt culture medium culturing of the present invention, photosynthetic bacterium concentration is high, OD
660up to 2.2.
Accompanying drawing explanation
Thalli growth situation when Fig. 1 is the embodiment of the present invention 1 culture medium culturing photosynthetic bacterium.
Thalli growth situation when Fig. 2 is the embodiment of the present invention 2 culture medium culturing photosynthetic bacterium.
Embodiment
The experimental technique used in following embodiment if no special instructions, is ordinary method.
Material used in following embodiment, reagent etc., if no special instructions, all can obtain from commercial channels.
Embodiment 1, prepare photosynthetic bacteria culture medium
Take following component: 6g sodium acetate trihydrate, 2g ammonium sulfate, 2g sodium bicarbonate, 0.4g potassium primary phosphate, 0.4g magnesium sulfate.
Prepare as follows:
Measure about 500ml deionized water, precise 6g sodium acetate trihydrate is added to the water dissolving, precise 2g ammonium sulfate is added to the water dissolving, precise 2g sodium bicarbonate is added to the water dissolving, precise 0.4g potassium primary phosphate is added to the water dissolving, and precise 0.4g magnesium sulfate is added to the water dissolving.Then in solution, supplement deionized water to about 990ml, stir.Use sodium hydroxide and hydrochloric acid conditioning solution pH to 7.5.Finally solution is settled to 1L.
The cost compare of substratum of the present invention and RCVBN substratum, as shown in Table 1 and Table 2:
The cost of table 1 embodiment of the present invention 1 substratum
The cost of table 2RCVBN substratum
Data in contrast table 1 and table 2 can draw, substratum of the present invention significantly reduces production cost; Compared with RCVBN substratum, the expense of output 1 kilogram of yeast culture base can save 85%.
The substratum of above-mentioned preparation is utilized to carry out the cultivation of photosynthetic bacterium:
Get cultivation that certain volume prepares based in Erlenmeyer flask, according to 30% photosynthetic bacterium finished product bacterium liquid, (wherein photosynthetic bacterium is rhodospirillum, rhodopseudomonas, outer sulphur Rhodopseudomonas palustris; Purchased from Beijing fishing through biotechnology limited liability company): 70% substratum (volume ratio) is inoculated.。Use oxygen flow sealed membrane to be sealed by Erlenmeyer flask after inoculation, leave standstill or shaking table shaking culture under being placed in 60W incandescent light, intensity of illumination controls at 3000lux, and temperature controls at 26 ~ 30 DEG C.Cultivate and can obtain finished product bacterium in 2 ~ 3 days.
In above-mentioned culturing process, as shown in Figure 1, as seen from Figure 1, the concentration of the photosynthetic bacterium of culture medium culturing of the present invention is high, OD for thalli growth situation
660up to 2.1, meet or exceed the cell concentration (be about 1.8, its culture scheme is identical with the culture scheme of substratum of the present invention) of RCVBN substratum gained.
Embodiment 2, prepare photosynthetic bacteria culture medium
Take following component: take following component: 10g sodium acetate trihydrate, 5g ammonium sulfate, 5g sodium bicarbonate, 0.8g potassium primary phosphate, 0.8g magnesium sulfate.
Prepare as follows:
Measure about 500ml deionized water, precise 10g sodium acetate trihydrate is added to the water dissolving, precise 5g ammonium sulfate is added to the water dissolving, precise 5g sodium bicarbonate is added to the water dissolving, precise 0.8g potassium primary phosphate is added to the water dissolving, and precise 0.8g magnesium sulfate is added to the water dissolving.Then in solution, supplement deionized water to about 990ml, stir.Use sodium hydroxide and hydrochloric acid conditioning solution pH to 7.5.Finally solution is settled to 1L.
The cost compare of substratum of the present invention and RCVBN substratum, as shown in table 3 and table 2:
The cost of table 3 embodiment of the present invention 2 substratum
Data in contrast table 3 and table 2 can draw, substratum of the present invention significantly reduces production cost; Compared with RCVBN substratum, the expense of output 1 kilogram of yeast culture base can save about 70%.
The substratum of above-mentioned preparation is utilized to carry out the cultivation of photosynthetic bacterium:
Get cultivation that certain volume prepares based in Erlenmeyer flask, according to 30% photosynthetic bacterium finished product bacterium liquid, (wherein photosynthetic bacterium is rhodospirillum, rhodopseudomonas, outer sulphur Rhodopseudomonas palustris; Purchased from Beijing fishing through biotechnology limited liability company): 70% substratum (volume ratio) is inoculated.Wherein photosynthetic bacterium is rhodospirillum, rhodopseudomonas, outer sulphur Rhodopseudomonas palustris.Use oxygen flow sealed membrane to be sealed by Erlenmeyer flask after inoculation, leave standstill or shaking table shaking culture under being placed in 60W incandescent light, intensity of illumination controls at 3000lux, and temperature controls at 26 ~ 30 DEG C.Cultivate and can obtain finished product bacterium in 2 ~ 3 days.
In above-mentioned culturing process, as shown in Figure 2, as seen from Figure 2, the concentration of the photosynthetic bacterium of culture medium culturing of the present invention is high, OD for thalli growth situation
660up to 2.2, meet or exceed the cell concentration (be about 1.8, its culture scheme is identical with the culture scheme of substratum of the present invention) of RCVBN substratum gained.
Claims (10)
1. a photosynthetic bacteria culture medium, is characterized in that: consisting of of photosynthetic bacteria culture medium described in every L:
Sodium acetate trihydrate 4 ~ 10g, ammonium sulfate 1 ~ 5g, sodium bicarbonate 1 ~ 5g, potassium primary phosphate 0.2 ~ 0.8g, magnesium sulfate 0.2 ~ 0.8g, and the water of surplus.
2. substratum according to claim 1, is characterized in that: consisting of of photosynthetic bacteria culture medium described in every L:
Sodium acetate trihydrate 6 ~ 10g, ammonium sulfate 2 ~ 5g, sodium bicarbonate 2 ~ 5g, potassium primary phosphate 0.4 ~ 0.8g, magnesium sulfate 0.4 ~ 0.8g, and the water of surplus.
3. substratum according to claim 1 and 2, is characterized in that: the pH value of described photosynthetic bacteria culture medium is 5.5 ~ 8.5.
4. the substratum according to Claims 2 or 3, is characterized in that: described in every L, photosynthetic bacteria culture medium consists of following 1) or 2):
1) sodium acetate trihydrate 6g, ammonium sulfate 2g, sodium bicarbonate 2g, potassium primary phosphate 0.4g, magnesium sulfate 0.4g, and the water of surplus;
2) sodium acetate trihydrate 10g, ammonium sulfate 5g, sodium bicarbonate 5g, potassium primary phosphate 0.8g, magnesium sulfate 0.8g, and the water of surplus.
5. the substratum according to any one of claim 1-4, is characterized in that: the pH value of described photosynthetic bacteria culture medium is 7.5.
6. the preparation method of substratum according to any one of claim 1-5, comprise the steps: described sodium acetate trihydrate, described ammonium sulfate, described sodium bicarbonate, described potassium primary phosphate and described magnesium sulfate to be dissolved in the water, after constant volume, namely obtain described substratum.
7. preparation method according to claim 5, is characterized in that: described method also comprises the step of described substratum being carried out to pH value regulation and control.
8. preparation method according to claim 7, is characterized in that: utilize sodium hydroxide and hydrochloric acid to regulate and control the pH value of described substratum.
9. the application of substratum according to any one of claim 1-5 in culturing bacterium.
10. application according to claim 9, is characterized in that: described bacterium is photosynthetic bacterium.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543146A (en) * | 2016-02-02 | 2016-05-04 | 田维熙 | Preparation method of photosynthetic bacterium water aqua and special culture medium of photosynthetic bacterium water aqua |
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN109810915A (en) * | 2018-04-19 | 2019-05-28 | 湛江恒兴养殖技术服务有限公司 | A kind of photosynthetic bacteria culture simplicity spreads cultivation culture medium and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706933A (en) * | 2004-06-11 | 2005-12-14 | 郭伟光 | Prepn process of high-concentration photosynthesis bacterial prepn |
| CN101113418A (en) * | 2007-07-06 | 2008-01-30 | 辽宁大学 | Photosynthetic bacterium enriched culture medium |
| CN102911868A (en) * | 2012-09-28 | 2013-02-06 | 新奥科技发展有限公司 | Microorganism culture medium and microorganism culture method |
-
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- 2015-10-22 CN CN201510691293.7A patent/CN105199993A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1706933A (en) * | 2004-06-11 | 2005-12-14 | 郭伟光 | Prepn process of high-concentration photosynthesis bacterial prepn |
| CN101113418A (en) * | 2007-07-06 | 2008-01-30 | 辽宁大学 | Photosynthetic bacterium enriched culture medium |
| CN102911868A (en) * | 2012-09-28 | 2013-02-06 | 新奥科技发展有限公司 | Microorganism culture medium and microorganism culture method |
Non-Patent Citations (3)
| Title |
|---|
| 张红莲: "富集光合细菌培养基的优化研究", 《水产科学》 * |
| 李亚丽: "光合细菌菌落特性的研究现状", 《农业技术与装备》 * |
| 洪坚平等: "《应用微生物学》", 28 February 2011 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105543146A (en) * | 2016-02-02 | 2016-05-04 | 田维熙 | Preparation method of photosynthetic bacterium water aqua and special culture medium of photosynthetic bacterium water aqua |
| CN105543146B (en) * | 2016-02-02 | 2018-11-13 | 田维熙 | A kind of preparation method and its special culture media of photosynthetic bacteria aqua |
| CN108220203A (en) * | 2018-03-06 | 2018-06-29 | 上海海洋大学 | A kind of fermentation medium of hydrogenlike silicon ion |
| CN108220203B (en) * | 2018-03-06 | 2021-03-02 | 上海海洋大学 | Fermentation medium of rhodobacter sphaeroides and application of fermentation medium in fermentation production of rhodobacter sphaeroides |
| CN109810915A (en) * | 2018-04-19 | 2019-05-28 | 湛江恒兴养殖技术服务有限公司 | A kind of photosynthetic bacteria culture simplicity spreads cultivation culture medium and preparation method thereof |
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Application publication date: 20151230 |