CN1706933A - Prepn process of high-concentration photosynthesis bacterial prepn - Google Patents
Prepn process of high-concentration photosynthesis bacterial prepn Download PDFInfo
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- CN1706933A CN1706933A CN 200410048678 CN200410048678A CN1706933A CN 1706933 A CN1706933 A CN 1706933A CN 200410048678 CN200410048678 CN 200410048678 CN 200410048678 A CN200410048678 A CN 200410048678A CN 1706933 A CN1706933 A CN 1706933A
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Abstract
The preparation process of high-concentration photosynthesis bacterial preparation includes the following steps: adding fermentation promoter and papaya extracting liquid into basic photosynthesis bacterial culture medium to prepare bacteria culture liquid, adding strain in 10-20 vol% of the culture liquid, setting into transparent container, and micro-aerobic culturing at temperature of 28-35 deg.c and illumination intensity of 1500-2500 LX for 72-120 hr to prepare photosynthesis bacterial preparation with bacteria content over 3 billion/ml. The micro-aerobic culturing condition is formed by means of stopping the mouth of the culture container with sterilized cotton stopper and shaking the container once every 5-8 hr. compared with common culturing process, the present invention has simple operation, short culture period, fast growth speed, high thallus yield, stable preparation quality and certain bacteriostasis.
Description
Technical field
The present invention relates to the preparation method of high density photosynthetic bacteria preparation.
Background technology
The preparation method of photosynthetic bacteria preparation is a lot, as publication number is that the method for the Chinese patent introduction of CN1104250A is: the mixed bacterial of the two strain bacterial strains of inoculation more than 30% in bacteria culture fluid, postvaccinal nutrient solution is 26-32 ℃ in temperature, intensity of illumination is 3000-5000LX, pH is 6-8, anaerobic condition was cultivated 48-72 hour down, then with the bacterium liquid of turning out in the system of opening wide, under the natural lighting, add once above carbon source according to the carbon source remaining quantity in the bacterium liquid in good time, in temperature is 20-35 ℃, aerobic venting condition is cultivated after 72-120 hour down, collect the packing of bacterium liquid, make the liquid microbe mixed preparation.Also have plenty of and in culturing process, carry out regularly stirring (is the Chinese patent of CN1400303A as publication number), as seen, most photosynthetic bacteria preparation preparation methods' focal point all is the control that concentrates on carbon source, intensity of illumination, temperature, oxygen level and pH value, carry out microbial inoculum production by adding several different methods such as carbon source, oxygenation, stirring, these methods one are to need to add carbon source aborning, charge into oxygen, operating process is loaded down with trivial details, and needs certain device; The 2nd, easy bacteria infection influences the microbial inoculum quality; The 3rd, incubation time is long, and thalline content is low, and quality is difficult to ensure.
Summary of the invention
The objective of the invention is to overcome above-mentioned the deficiencies in the prior art, a kind of fast growth, thalline content height are provided, have the preparation method of the high density photosynthetic bacteria preparation of bacteriostatic action.
The technical solution adopted in the present invention is: add leaven promoting agent and pawpaw extracting solution and make thalline production nutrient solution in the photosynthetic bacterium minimum medium, add the bacterial classification that accounts for bacterial culture fluid cumulative volume 10～20% again, pack into after mixing in the transparent vessel, little aerobic cultivation is 72～120 hours under the condition of 28～35 ℃ of temperature, intensity of illumination 1500～2500LX, makes the photosynthetic microbial inoculum of viable bacteria content more than 3,000,000,000/ml.
The equal buyable of photosynthetic bacterium bacterial classification described in the present invention adopts two or more bacterial strain to carry out mixed culture at least, and inoculum density is more than 3,000,000,000/ml.
Consisting of of described photosynthetic bacterium minimum medium: yeast extract paste 1～2g, potassium primary phosphate 0.4～0.6g, sal epsom 0.3～0.6g, sodium acetate 2～4g, water 1000g, pH value 6.5～7.5.
Leaven promoting agent described in the present invention is meant xanthohumic acid.The addition that xanthohumic acid is produced in the nutrient solution at thalline is 0.05～0.1%.
More than said pawpaw extracting solution be meant: after fresh pawpaw is rubbed, add the water of 3 times of weight, simmer in water and boil half an hour, the stoste that obtains after the filtration.The addition of pawpaw extracting solution is 0.5～1.5%.
More than said transparent vessel be meant transparent plastic containers or Glass Containers.
More than said little aerobic cultivation be meant that the yeast culture container seals with the sterilization tampon, shakes up once every 5～8 hours in the culturing process.
More than raw material that said bacterial culture fluid adopted, if be used for agricultural planting or domestic refuse, sewage disposal, then adopt industrial raw material; If be used for brutish raising, aquaculture, then adopt food grade materials; Water can adopt clean river, underground water, tap water etc., but every indexs such as its hardness, coliform count, BOD value, cl content all should satisfy the requirement of general microorganism growth.
The xanthohumic acid that is added among the present invention can obviously promote the speed of growth of photosynthetic bacterium, shortens the production time, improves the cell density in the nutrient solution; Contain effective constituents such as abundant VITAMIN, protein, organic acid, Fructus Chaenomelis ferment in the pawpaw extracting solution, these compositions all can be utilized by photosynthetic bacterium, for photosynthetic bacterium growth provides competent nutrient.In addition, the organic acid that contains in xanthohumic acid and the pawpaw is weak acid, can play shock absorption in nutrient solution, and the pH value is kept relative stability, and helps the growth of photosynthetic bacterium.In addition, Fructus Chaenomelis ferment can suppress varied bacteria growing, improves the activity of photosynthetic bacterium.
The present invention compares with general cultural method, and its advantage is: the one, need not in the process of growth to add carbon source and oxygenation, and simple to operate; The 2nd, incubation time shortens to 3～5 days, has improved production efficiency; The 3rd, thalline yield height, thalline content is more than 3 ‰ in the finished product; The 4th, there is not assorted bacterium substantially, certain bacteriostatic action is arranged.
Embodiment
The present invention is further illustrated below by embodiment.
Embodiment 1:
1. preparation bacterial classification: photosynthetic bacterium is adopted capsula Rhodopseudomonas Rhodopseudomonas capsulata and Rhodopseudomonas palustris Rps.Palustris, mixes obtaining mixed strains after these two bacterial strains are cultivated respectively in 1: 1 by volume;
2. preparation nutrient solution: take by weighing yeast extract paste 1.5kg, potassium primary phosphate 0.5kg, sal epsom 0.3kg, sodium acetate 2.5kg, xanthohumic acid 0.8kg is dissolved in respectively in the 1000kg water, adds pawpaw extracting solution 1kg again, and adjusting the pH value is 7.0;
3. cultivation bacterial classification: the 180kg mixed strains is added in the nutrient solution, mix, pack in the transparent plastic tank, tampon beyond the Great Wall, under the condition of 32～35 ℃ of temperature, intensity of illumination 2000LX, cultivated 3～5 days, shook up once every 5 hours in the culturing process,, make photosynthetic microbial inoculum when viable bacteria concentration in the bacterium liquid reaches 3,000,000,000/ml when above.
Embodiment 2:
1. preparation bacterial classification: photosynthetic bacterium is adopted capsula Rhodopseudomonas Rhodobacter capsulatus, Rhodopseudomonas palustris Rps.rutila and Rhodopseudomonas spheroides Rps.Globiformis.Mixed in 1: 1: 1 by volume after three bacterial strains are cultivated respectively and obtain mixed strains;
2. preparation nutrient solution: take by weighing yeast extract paste 2kg, potassium primary phosphate 0.5kg, sal epsom 0.5kg, sodium acetate 3kg, xanthohumic acid 0.5kg is dissolved in respectively in the 1000kg water, adds pawpaw extracting solution 1.5kg again, and adjusting the pH value is 7.0;
3. cultivation bacterial classification: the 200kg mixed strains is added in the nutrient solution, mix, pack in the transparent plastic tank, tampon beyond the Great Wall, under the condition of 32～35 ℃ of temperature, intensity of illumination 2500LX, cultivated 4 days, shook up once every 5 hours in the culturing process,, make photosynthetic microbial inoculum when viable bacteria concentration in the bacterium liquid reaches 3,000,000,000/ml when above.
Claims (5)
1. the preparation method of a high density photosynthetic bacteria preparation, it is characterized in that, in the photosynthetic bacterium minimum medium, add leaven promoting agent and pawpaw extracting solution and make thalline production nutrient solution, add the bacterial classification that accounts for bacterial culture fluid cumulative volume 10～20% again, pack into after mixing in the transparent vessel, little aerobic cultivation is 72～120 hours under the condition of 28～35 ℃ of temperature, intensity of illumination 1500～2500LX, makes the photosynthetic microbial inoculum of viable bacteria content more than 3,000,000,000/ml.
2. the preparation method of high density photosynthetic bacteria preparation according to claim 1 is characterized in that leaven promoting agent is meant xanthohumic acid, and its addition of producing in the nutrient solution at thalline is 0.05～0.1%.
3. the preparation method of high density photosynthetic bacteria preparation according to claim 1, it is characterized in that, after the pawpaw extracting solution is meant that fresh pawpaw is rubbed, the water that adds 3 times of weight, simmer in water and boil half an hour, the stoste that obtains after the filtration, its addition of producing in the nutrient solution at thalline is 0.5～1.5%.
4. the preparation method of high density photosynthetic bacteria preparation according to claim 1 is characterized in that, the consisting of of photosynthetic bacterium minimum medium: yeast extract paste 1～2g, potassium primary phosphate 0.4～0.6g, sal epsom 0.3～0.6g, sodium acetate 2～4g, water 1000g, pH value 6.5～7.5.
5. the preparation method of high density photosynthetic bacteria preparation according to claim 1 is characterized in that, little aerobic cultivation is meant that the yeast culture container seals with the sterilization tampon, shakes up once every 5～8 hours in the culturing process.
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| CN 200410048678 CN1706933A (en) | 2004-06-11 | 2004-06-11 | Prepn process of high-concentration photosynthesis bacterial prepn |
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| CN 200410048678 CN1706933A (en) | 2004-06-11 | 2004-06-11 | Prepn process of high-concentration photosynthesis bacterial prepn |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102061279A (en) * | 2010-11-22 | 2011-05-18 | 河北省微生物研究所 | Method for producing rhodopseudomonas palustris fermentation liquor by high-density fermentation |
| CN101748092B (en) * | 2010-02-05 | 2011-12-28 | 北京科技大学 | Method for efficiently culturing photosynthetic bacteria by utilizing molasses |
| CN105199993A (en) * | 2015-10-22 | 2015-12-30 | 中国人民大学 | Photosynthetic bacteria culture medium and preparation method thereof |
| CN106635928A (en) * | 2017-02-13 | 2017-05-10 | 天津市农业生物技术研究中心 | Culture method for continuous and scaled production of photosynthetic bacteria |
| CN106967642A (en) * | 2017-04-13 | 2017-07-21 | 北京柯林沃德科技有限公司 | A kind of water remediation method based on Composite Photosynthetic Bacteria preparation |
-
2004
- 2004-06-11 CN CN 200410048678 patent/CN1706933A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101748092B (en) * | 2010-02-05 | 2011-12-28 | 北京科技大学 | Method for efficiently culturing photosynthetic bacteria by utilizing molasses |
| CN102061279A (en) * | 2010-11-22 | 2011-05-18 | 河北省微生物研究所 | Method for producing rhodopseudomonas palustris fermentation liquor by high-density fermentation |
| CN105199993A (en) * | 2015-10-22 | 2015-12-30 | 中国人民大学 | Photosynthetic bacteria culture medium and preparation method thereof |
| CN106635928A (en) * | 2017-02-13 | 2017-05-10 | 天津市农业生物技术研究中心 | Culture method for continuous and scaled production of photosynthetic bacteria |
| CN106967642A (en) * | 2017-04-13 | 2017-07-21 | 北京柯林沃德科技有限公司 | A kind of water remediation method based on Composite Photosynthetic Bacteria preparation |
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