CN103103119B - Continuous quantitative microbial inoculation device - Google Patents

Continuous quantitative microbial inoculation device Download PDF

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Publication number
CN103103119B
CN103103119B CN201310032714.6A CN201310032714A CN103103119B CN 103103119 B CN103103119 B CN 103103119B CN 201310032714 A CN201310032714 A CN 201310032714A CN 103103119 B CN103103119 B CN 103103119B
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hinge
inoculation
wing
head cavity
inoculation head
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CN201310032714.6A
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CN103103119A (en
Inventor
王保忠
谢桂林
裴殿兴
郝建华
谢桐品
丁峰
程波
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Changshu Zijin Intellectual Property Service Co.,Ltd.
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Changshu Nanjing Normal University Development Research Institute Co Ltd
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Abstract

The invention relates to a continuous quantitative microbial inoculation device. The continuous quantitative microbial inoculation device comprises an inoculation head, an inoculation neck, an air control valve, an air inlet valve, an injection pump, a push piston of the injection pump, a hinge wing rear edge, a head-neck connector, an inoculation head cavity rear opening, a closed wing fixing strip, a closed wing, an inoculation head cavity, an inoculation head cavity wall, an inoculation head cavity front opening, a hinge wing shaft, a hinge break joint, a hinge, a hinge wing, a hinge upper surface, a hinge upper surface rotary drum, a hinge lower surface rotary drum, a hinge upper surface rotary drum joint and a hinge lower surface, wherein the inoculation head is of a structure for scraping or sucking a strain and comprises an upper part and a lower part, the upper part and the lower part are connected together through the hinge wing shaft, and under the combined action of the hinge and the closed wing fixing strip, an outward bulge at the center, namely the inoculation head cavity wall can form the inoculation head cavity at the center of the inoculation head, and the hinge wing and the closed wing are formed on the two sides so as to increase the closing performance of the upper part and the lower part of the inoculation head cavity after the upper part and the lower part are closed. The inoculation device is simple to manufacture, strong in operability, low in cost and obvious in effects.

Description

Quantitatively continuous microbial inoculant device
Technical field
The present invention relates to a kind of quantitatively continuous microbial inoculant device, belong to medical treatment and laboratory equipment technical field.
Background technology
In microbiological research process, inoculator is one of experimental tool of operator's indispensability, traditional inoculator is to fix a thin wire an iron rod one end, sometimes iron wire end hook, there is the hour circle circlewise, thereby as much as possible bacterial classification is provoked, and then transfer to the object on another flat board.Such operation often can only be inoculated operation off and on, required time is longer, and inoculation environment tends to impact to people's health, various medicines and utensil also may damage human body, add that microorganism individuality is less, in the amount that with the naked eye can observe, just contain the microorganism of enormous quantity, so traditional inoculator can only operate off and on, large usage quantity, waste also greatly, and can not meet rapidly the quantitatively requirement of vaccinization.Design a kind of utensil can be rapidly quantitatively vaccinization become and be badly in need of a great problem that solves, so utilize syringe can be quantitatively, inoculation head a kind of quantitative vaccinization device of quantitative vaccinization rapidly of characteristic invention that can store relatively large bacterial classification is necessary.
Summary of the invention
Can not meet in microbiological research process rapidly a quantitatively difficult problem for the requirement of vaccinization in order to solve traditional inoculator, the invention provides a kind of quantitatively vaccinization device, this quantitative vaccinization device utilize syringe can be quantitatively, inoculation head can store the characteristic of relatively large bacterial classification, is able to rapidly the quantitatively object of vaccinization.
The technical solution adopted for the present invention to solve the technical problems is:
Quantitatively vaccinization device is by inoculating 1, inoculation neck 2, control valve 3, intake valve 4, injection pump 5, injection pump plunger 6, hinge wing trailing edge 7, neck connects 8, inoculation head cavity deutostoma 9, the solid bar 10 of the closed wing, the closed wing 11, inoculation head cavity 12, inoculation head cavity wall 13, inoculation head cavity prosopyle 14, hinge wing axle 15, hinge break joint 16, hinge 17, the hinge wing 18, above hinge 19, rotating cylinder 20 above hinge, rotating cylinder 21 below hinge, rotating cylinder seam 22 above hinge, 23 compositions below hinge, inoculation 1 is scraping or the structure of drawing bacterial classification, comprise upper and lower two portions, upper and lower two portions are linked together by hinge wing axle 15, under the acting in conjunction of hinge 17 and the solid bar 10 of the closed wing, projection outside mediad inoculates head cavity wall 13 can form inoculation head cavity 12 in inoculation 1 central authorities, both sides form the hinge wing 18 and the closed wing 11, to increase inoculation head cavity two portions airtight performance after closing up Shang Xia 12.
The tinplate sheet that described inoculation 1 is 1 millimeter by thickness is made, and length is 1-2 centimetre.
The edge of the described hinge wing 18 is surrounded by hinge break joint 16, hinge 17 and hinge wing trailing edge 7, be the hinge wing Shang Xia 18 two portions closely close up the engagement edge of rear formation.The structure of hinge 17 is with known hinge, comprise hinge wing axle 15, rotating cylinder 21 below rotating cylinder 20, hinge above hinge 19, above hinge, above hinge below rotating cylinder seam 22 and hinge 23, can guarantee inoculation 1 Shang Xia two portions freely separate and closure.
The solid bar 10 of the described closed wing is to be welded on the closed wing 11 strip iron plate of trailing edge below, length is 1 centimetre, and width is 2 millimeters, when inoculation is Shang Xia 1 after two portions closure, with tweezers, the closed wing is consolidated to bar 10 nests and above trailing edge, reach the object of the fixing closed wing 11 on the closed wing 11.
The circular open of described inoculation head cavity 12 front ends is inoculation head cavity prosopyle 14, and diameter is 1-5 millimeter, and the circular open of inoculation head cavity 12 rear ends is inoculation head cavity deutostoma 9, and diameter is 3-8 millimeter.
Described inoculation neck 2 is that length is that 10-15 centimetre, outside diameter are that 3-8 millimeter, interior diameter are the stainless steel tube of 1-5 millimeter, front end puts in after inoculation head cavity 121-3 millimeter, weld together with the inner side of inoculation head cavity wall 13 lower parts of inoculating head cavity deutostoma 9 places, form neck and connect 8.In the pipe of inoculation neck 2 rear end 2 centimeters, have a control valve 3, its structure, with known Non-return air valve, can allow gas to blow to front end and stop gas to be back to rear end from front end from rear end.Apart from the outwardly intake valve 4 of inoculation neck 2 rear end 1 centimeters, its structure is with the breather cheek valve of known intravenous injector.
Principle of work of the present invention is: in the time that injection pump plunger 6 fills gas to the outer twitch of injection pump 5 for injection pump 5 inner chambers, intake valve 4 is opened, and gas enters injection pump 5 thus; Injecting pump plunger 6 to the interior promotion of injection pump 5, when gas is blowed to inoculation neck 2, intake valve 4 closures, gas enters inoculation neck 2 thus, and then effect inoculation head cavity 12, the bacterial classification in inoculation head cavity 12 is blown to planar surface to be inoculated from inoculation head cavity prosopyle 14.Because the bacterial classification amount in inoculation head cavity 12 is more, on injection pump 5, have scale, so just quantitatively distance ground promotes injection pump plunger 6, and bacterial classification is quantitatively blown out to inoculation head cavity 12, and can be multiple platings to be inoculated continuously, reach the object of quantitative vaccinization.
Beneficial effect of the present invention is, quantitatively vaccinization device utilize syringe can be quantitatively, inoculation head can store the characteristic of relatively large bacterial classification, is able to the object of quantitative vaccinization rapidly.Quantitatively vaccinization device is made simply, and diverse in function is workable, with low cost, successful.
accompanying drawing explanation
Below in conjunction with accompanying drawing, the present invention is further described.
Fig. 1 is the one-piece construction schematic diagram of the quantitative vaccinization device of band of the present invention.
Fig. 2 is the vertical section structure schematic diagram of the quantitative vaccinization device of band of the present invention.
Fig. 3 is the inoculation header structure schematic diagram of the quantitative vaccinization device of band of the present invention.
Fig. 4 is the hinge structure schematic diagram of the inoculation head of the quantitative vaccinization device of band of the present invention.
1. inoculation heads, 2. inoculation neck, 3. control valve in figure, 4. intake valve, 5. injection pump, 6. injection pump plunger, 7. hinge wing trailing edge, 8. neck connects, 9. inoculation head cavity deutostoma, 10. the solid bar of the closed wing, the 11. closed wings, 12. inoculation head cavitys, 13. inoculation head cavity walls, 14. inoculation head cavity prosopyles, 15. hinge wing axles, 16. hinge break joints, 17. hinges, the 18. hinge wings, above 19. hinges, rotating cylinder above 20. hinges, rotating cylinder below 21. hinges, rotating cylinder seam above 22. hinges, below 23. hinges.
Embodiment
Embodiment mono-:
Quantitatively vaccinization device is by inoculating 1, inoculation neck 2, control valve 3, intake valve 4, injection pump 5, injection pump plunger 6, hinge wing trailing edge 7, neck connects 8, inoculation head cavity deutostoma 9, the solid bar 10 of the closed wing, the closed wing 11, inoculation head cavity 12, inoculation head cavity wall 13, inoculation head cavity prosopyle 14, hinge wing axle 15, hinge break joint 16, hinge 17, the hinge wing 18, above hinge 19, rotating cylinder 20 above hinge, rotating cylinder 21 below hinge, rotating cylinder seam 22 above hinge, 23 compositions below hinge, it is characterized in that inoculation 1 is the structure of scraping bacterial classification, the tinplate sheet that is 1 millimeter by thickness is made, length is 1 centimetre, comprise upper and lower two portions, upper and lower two portions are linked together by hinge wing axle 15, under the acting in conjunction of hinge 17 and the solid bar 10 of the closed wing, projection outside mediad inoculates head cavity wall 13 can form inoculation head cavity 12 in inoculation 1 central authorities, both sides form the hinge wing 18 and the closed wing 11, to increase inoculation head cavity two portions airtight performance after closing up Shang Xia 12.
The edge of the hinge wing 18 is surrounded by hinge break joint 16, hinge 17 and hinge wing trailing edge 7, be the hinge wing Shang Xia 18 two portions closely close up the engagement edge of rear formation.The structure of hinge 17 is with known hinge, comprise hinge wing axle 15, rotating cylinder 21 below rotating cylinder 20, hinge above hinge 19, above hinge, above hinge below rotating cylinder seam 22 and hinge 23, can guarantee inoculation 1 Shang Xia two portions freely separate and closure.
The solid bar 10 of the closed wing is to be welded on the closed wing 11 strip iron plate of trailing edge below, length is 1 centimetre, and width is 2 millimeters, when inoculation is Shang Xia 1 after two portions closure, with tweezers, the closed wing is consolidated to bar 10 nests and above trailing edge, reach the object of the fixing closed wing 11 on the closed wing 11.
The circular open of inoculation head cavity 12 front ends is inoculation head cavity prosopyle 14, and diameter is 1-5 millimeter, and the circular open of inoculation head cavity 12 rear ends is inoculation head cavity deutostoma 9, and diameter is 3-8 millimeter.
Inoculation neck 2 is that length is that 10-15 centimetre, outside diameter are that 3-8 millimeter, interior diameter are the stainless steel tube of 1-5 millimeter, front end puts in after inoculation head cavity 121-3 millimeter, weld together with the inner side of inoculation head cavity wall 13 lower parts of inoculating head cavity deutostoma 9 places, form neck and connect 8.In the pipe of inoculation neck 2 rear end 2 centimeters, have a control valve 3, its structure, with known Non-return air valve, can allow gas to blow to front end and stop gas to be back to rear end from front end from rear end.Apart from the outwardly intake valve 4 of inoculation neck 2 rear end 1 centimeters, its structure is with the breather cheek valve of known intravenous injector.
In the time that injection pump plunger 6 fills gas to the outer twitch of injection pump 5 for injection pump 5 inner chambers, intake valve 4 is opened, and gas enters injection pump 5 thus; Injecting pump plunger 6 to the interior promotion of injection pump 5, when gas is blowed to inoculation neck 2, intake valve 4 closures, gas enters inoculation neck 2 thus, and then effect inoculation head cavity 12, the bacterial classification in inoculation head cavity 12 is blown to planar surface to be inoculated from inoculation head cavity prosopyle 14.Because the bacterial classification amount in inoculation head cavity 12 is more, on injection pump 5, have scale, so just quantitatively distance ground promotes injection pump plunger 6, and bacterial classification is quantitatively blown out to inoculation head cavity 12, and can be multiple platings to be inoculated continuously, reach the object of quantitative vaccinization.
Embodiment bis-:
Quantitatively vaccinization device is by inoculating 1, inoculation neck 2, control valve 3, intake valve 4, injection pump 5, injection pump plunger 6, hinge wing trailing edge 7, neck connects 8, inoculation head cavity deutostoma 9, the solid bar 10 of the closed wing, the closed wing 11, inoculation head cavity 12, inoculation head cavity wall 13, inoculation head cavity prosopyle 14, hinge wing axle 15, hinge break joint 16, hinge 17, the hinge wing 18, above hinge 19, rotating cylinder 20 above hinge, rotating cylinder 21 below hinge, rotating cylinder seam 22 above hinge, 23 compositions below hinge, it is characterized in that inoculation 1 is the structure of drawing bacterial classification, the tinplate sheet that is 1 millimeter by thickness is made, length is 2 centimetres, comprise upper and lower two portions, upper and lower two portions are linked together by hinge wing axle 15, under the acting in conjunction of hinge 17 and the solid bar 10 of the closed wing, projection outside mediad inoculates head cavity wall 13 can form inoculation head cavity 12 in inoculation 1 central authorities, both sides form the hinge wing 18 and the closed wing 11, to increase inoculation head cavity two portions airtight performance after closing up Shang Xia 12.
The edge of the hinge wing 18 is surrounded by hinge break joint 16, hinge 17 and hinge wing trailing edge 7, be the hinge wing Shang Xia 18 two portions closely close up the engagement edge of rear formation.The structure of hinge 17 is with known hinge, comprise hinge wing axle 15, rotating cylinder 21 below rotating cylinder 20, hinge above hinge 19, above hinge, above hinge below rotating cylinder seam 22 and hinge 23, can guarantee inoculation 1 Shang Xia two portions freely separate and closure.
The solid bar 10 of the closed wing is to be welded on the closed wing 11 strip iron plate of trailing edge below, length is 1 centimetre, and width is 2 millimeters, when inoculation is Shang Xia 1 after two portions closure, with tweezers, the closed wing is consolidated to bar 10 nests and above trailing edge, reach the object of the fixing closed wing 11 on the closed wing 11.
The circular open of inoculation head cavity 12 front ends is inoculation head cavity prosopyle 14, and diameter is 1-5 millimeter, and the circular open of inoculation head cavity 12 rear ends is inoculation head cavity deutostoma 9, and diameter is 3-8 millimeter.
Inoculation neck 2 is that length is that 10-15 centimetre, outside diameter are that 3-8 millimeter, interior diameter are the stainless steel tube of 1-5 millimeter, front end puts in after inoculation head cavity 121-3 millimeter, weld together with the inner side of inoculation head cavity wall 13 lower parts of inoculating head cavity deutostoma 9 places, form neck and connect 8.In the pipe of inoculation neck 2 rear end 2 centimeters, have a control valve 3, its structure, with known Non-return air valve, can allow gas to blow to front end and stop gas to be back to rear end from front end from rear end.Apart from the outwardly intake valve 4 of inoculation neck 2 rear end 1 centimeters, its structure is with the breather cheek valve of known intravenous injector.
In the time that injection pump plunger 6 fills gas to the outer twitch of injection pump 5 for injection pump 5 inner chambers, intake valve 4 is opened, and gas enters injection pump 5 thus; Injecting pump plunger 6 to the interior promotion of injection pump 5, when gas is blowed to inoculation neck 2, intake valve 4 closures, gas enters inoculation neck 2 thus, and then effect inoculation head cavity 12, the bacterial classification in inoculation head cavity 12 is blown to planar surface to be inoculated from inoculation head cavity prosopyle 14.Because the bacterial classification amount in inoculation head cavity 12 is more, on injection pump 5, have scale, so just quantitatively distance ground promotes injection pump plunger 6, and bacterial classification is quantitatively blown out to inoculation head cavity 12, and can be multiple platings to be inoculated continuously, reach the object of quantitative vaccinization.
More than show and described ultimate principle of the present invention and principal character and advantage of the present invention.The technician of the industry should be appreciated that; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification sheets, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications; these changes and improvements all fall in the claimed scope of the invention, and the claimed scope of the present invention is defined by its equivalent of appending claims.

Claims (3)

1. quantitatively continuous microbial inoculant device is by inoculating head (1), inoculation neck (2), control valve (3), intake valve (4), injection pump (5), injection pump plunger (6), hinge wing trailing edge (7), neck connects (8), inoculation head cavity deutostoma (9), the solid bar (10) of the closed wing, the closed wing (11), inoculation head cavity (12), inoculation head cavity wall (13), inoculation head cavity prosopyle (14), hinge wing axle (15), hinge break joint (16), hinge (17), the hinge wing (18), above hinge (19), rotating cylinder above hinge (20), rotating cylinder below hinge (21), rotating cylinder seam (22) above hinge, (23) composition below hinge, it is characterized in that inoculation head (1) is scraping or the structure of drawing bacterial classification, comprise upper and lower two portions, upper and lower two portions are linked together by hinge wing axle (15), under the acting in conjunction of hinge (17) and the solid bar (10) of the closed wing, projection outside mediad is inoculated head cavity wall (13) and is formed inoculation head cavity (12) in inoculation head (1) central authorities, both sides form the hinge wing (18) and the closed wing (11), to increase the airtight performance of the upper and lower two portions of inoculation head cavity (12) after closing up, the edge of the hinge wing (18) is by hinge break joint (16), hinge (17) and hinge wing trailing edge (7) surround, and are that the upper and lower two portions of the hinge wing (18) closely close up the engagement edge of rear formation, hinge (17) comprises hinge wing axle (15), (19) above hinge, rotating cylinder (21) below rotating cylinder (20), hinge above hinge, above hinge below rotating cylinder seam (22) and hinge (23), can guarantee that the upper and lower two portions of inoculation head (1) freely separate and closure, the solid bar (10) of the closed wing is to be welded on the closed wing (11) the strip iron plate of trailing edge below, when after the upper and lower two portions closure of inoculation head (1), by solid closed wing bar (10) nest trailing edge top on the closed wing (11), reach the object of the fixing closed wing (11) with tweezers, the circular open of inoculation head cavity (12) front end is inoculation head cavity prosopyle (14), and the circular open of inoculation head cavity (12) rear end is inoculation head cavity deutostoma (9), inoculation neck (2) is stainless steel tube, and front end puts in inoculation head cavity (12), welds together with the inner side of inoculation head cavity wall (13) lower part located of inoculation head cavity deutostoma (9), forms neck and connects (8), in inoculation neck (2) rear end pipe, there is a control valve (3), inoculation neck (2) the outwardly intake valve in rear end (4).
2. quantitatively continuous microbial inoculant device according to claim 1, is characterized in that inoculating the tinplate sheet that head (1) is 1 millimeter by thickness and makes, and length is 1-2 centimetre.
3. quantitatively continuous microbial inoculant device according to claim 1, is characterized in that solid bar (10) length of the closed wing is 1 centimetre, and width is 2 millimeters.
CN201310032714.6A 2013-01-29 2013-01-29 Continuous quantitative microbial inoculation device Active CN103103119B (en)

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CN103103119B true CN103103119B (en) 2014-07-02

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115109688A (en) * 2020-12-15 2022-09-27 山东省农业科学院畜牧兽医研究所 Inoculation instrument for muscle cell culture

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2033588U (en) * 1988-03-22 1989-03-08 宋树财 Birds embryo inoculating implement
CN2121804U (en) * 1992-04-11 1992-11-18 何彦彪 Mushroom vaccinator
CN2142004Y (en) * 1993-03-15 1993-09-15 吴国钧 Disposable mini quantitative implement for vaccinating
CN201545838U (en) * 2009-12-08 2010-08-11 史国平 Rapid inoculator
CN101940124A (en) * 2009-07-04 2011-01-12 甘肃省科学院生物研究所 Continuous quantitative adjustable inoculation gun for edible fungi
CN201830689U (en) * 2010-10-20 2011-05-18 马二宗 Inoculation gun

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN2033588U (en) * 1988-03-22 1989-03-08 宋树财 Birds embryo inoculating implement
CN2121804U (en) * 1992-04-11 1992-11-18 何彦彪 Mushroom vaccinator
CN2142004Y (en) * 1993-03-15 1993-09-15 吴国钧 Disposable mini quantitative implement for vaccinating
CN101940124A (en) * 2009-07-04 2011-01-12 甘肃省科学院生物研究所 Continuous quantitative adjustable inoculation gun for edible fungi
CN201545838U (en) * 2009-12-08 2010-08-11 史国平 Rapid inoculator
CN201830689U (en) * 2010-10-20 2011-05-18 马二宗 Inoculation gun

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Inventor after: Xie Guilin

Inventor after: Pei Dianxing

Inventor after: Hao Jianhua

Inventor after: Xie Tongpin

Inventor after: Ding Feng

Inventor after: Cheng Bo

Inventor before: Wang Baozhong

Inventor before: Xie Guilin

Inventor before: Pei Dianxing

Inventor before: Hao Jianhua

Inventor before: Xie Tongpin

Inventor before: Ding Feng

Inventor before: Cheng Bo

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Effective date of registration: 20201222

Address after: No.3 building, No.88 Xianshi Road, Changshu high tech Industrial Development Zone, Suzhou City, Jiangsu Province

Patentee after: Changshu Zijin Intellectual Property Service Co.,Ltd.

Address before: 215500 No.1 complex building, No.8 Jindu Road, Southeast Economic Development Zone, Changshu City, Suzhou City, Jiangsu Province

Patentee before: CHANGSHU NANJING NORMAL UNIVERSITY DEVELOPMENT RESEARCH INSTITUTE Co.,Ltd.