CN215404280U - Tumor cell culture device - Google Patents
Tumor cell culture device Download PDFInfo
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- CN215404280U CN215404280U CN202022589016.4U CN202022589016U CN215404280U CN 215404280 U CN215404280 U CN 215404280U CN 202022589016 U CN202022589016 U CN 202022589016U CN 215404280 U CN215404280 U CN 215404280U
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Abstract
The utility model discloses a tumor cell culture device, and relates to the technical field of cell culture devices. The utility model provides a tumor cell culture apparatus, including incubator and blake bottle, the lower part of incubator is equipped with magnetic field generating device, magnetic field generating device's top is equipped with the backup pad, it is connected with the erection column to rotate in the backup pad, be equipped with the draw-in groove on the erection column, the blake bottle is placed in the draw-in groove, the magnetism rotor has been placed in the blake bottle, the bottom outside of erection column is equipped with the sawtooth, the center of backup pad is equipped with the motor, the outside cover of motor has the protective housing, the protective housing surface is equipped with the electric plate, the lower extreme of motor is equipped with the pivot, the lower extreme of pivot is equipped with drive gear, drive gear meshes with the sawtooth on the erection column mutually, the top of incubator is equipped with sealed lid, the outer wall of blake box is equipped with control panel, the bottom of blake box is equipped with the universal wheel. The scheme of the utility model can meet the requirement of people on stable fixation and rotation of the tumor cell culture device and simultaneous culture of a plurality of samples.
Description
Technical Field
The utility model belongs to the technical field of cell culture devices, and relates to a tumor cell culture device.
Background
In modern society, cancer is a serious threat to human health and life, and is the first few causes of human death. Therefore, tumor cells play a central role in medical tissue culture, and tumor cell culture is an extremely important means for studying canceration mechanisms, anticancer drug detection and cancer molecular biology. In current tumour cell culture, often adopt tissue block culture method, the process of cultivateing needs to use devices such as blake bottle, and traditional blake bottle is inconvenient fixed, and because the reason of torticollis, the interval of not being convenient for places the tumour tissue fritter, and smearing of collagen thin layer is also very troublesome simultaneously.
In order to solve the above problems, the Chinese patent (publication No. CN207672074U) discloses a tumor cell culture device, which comprises a culture bottle, wherein the culture bottle body is cylindrical, the mouth of the culture bottle is fixedly communicated with an inclined neck, the outer end of the inclined neck is covered with a bottle mouth cover, the tumor cell culture device further comprises a rotary positioning support, the bottom of the culture bottle is arranged in an open manner, the bottom of the culture bottle is correspondingly provided with a push-pull rod, one end of the push-pull rod is fixed with a piston, an annular cotton ring is bonded and sleeved on the circumferential surface of the piston, the annular cotton ring is tightly plugged in the culture bottle, the other end of the push-pull rod is fixed with a bottle bottom cover, the rotary positioning support comprises a rubber block, a base, two shaft levers, two vertical plates, a first end gear, a second end gear and two pressure springs, a circular through groove is arranged in the center of the rubber block and used for plugging the culture bottle, the two shaft levers are horizontally and coaxially arranged, the rubber block is fixed between the two shaft levers, the two vertical plates are horizontally arranged in parallel, a round hole is correspondingly formed in the upper ends of the two vertical plates respectively, the outer ends of the two shaft levers are rotatably arranged in the two round holes respectively, a base is fixed at the lower ends of the two vertical plates, a first end gear and a second end gear are sleeved in the middle of the side wall of each shaft lever, each first end gear is meshed with the corresponding second end gear, each first end gear is fixedly connected with the corresponding shaft lever, each second end gear is slidably connected with the corresponding shaft lever, a pressure spring is further sleeved on the side wall of each shaft lever, each pressure spring is supported between the corresponding second end gear and the vertical plates, two limiting rod heads are vertically fixed at the upper end of the inner side surface of each vertical plate, each second end gear is provided with limiting holes corresponding to the two limiting rod heads one by one, and the outer ends of the two limiting rod heads of each vertical plate are inserted into the two limiting holes of the corresponding second end gears at intervals.
Although the above scheme solves the problems of fixing and rotating the culture bottle of the tumor cell culture device and placing the tumor tissue block, the method can only culture one sample, cannot simultaneously culture a plurality of samples, and performs contrast tests with different variables.
SUMMERY OF THE UTILITY MODEL
The utility model aims to provide a tumor cell culture device, which meets the requirement that the tumor cell culture device can be stably fixed and rotated and simultaneously culture a plurality of samples.
In order to solve the technical problems, the technical scheme of the utility model is as follows:
the utility model provides a tumor cell culture device, including incubator and blake bottle, the lower part of incubator is equipped with magnetic field generating device, magnetic field generating device's top is equipped with the backup pad, it has a plurality of round holes that the circumference was laid to open in the backup pad, the round hole internal rotation is connected with the erection column, the upper end of erection column is equipped with the draw-in groove, the blake bottle is placed in the draw-in groove, the magnetism rotor has been placed in the blake bottle, the bottom outside of erection column is equipped with the sawtooth, the center of backup pad is equipped with the motor, the outside cover of motor has the protective housing, the protective housing surface is equipped with the electric plate, the motor lower extreme is equipped with the pivot, the pivot lower extreme is equipped with drive gear, drive gear is located between group's magnetic field generating device and the backup pad, drive gear meshes with the sawtooth on the erection column mutually, the top of blake box is equipped with the sealed lid of rotation connection, the outer wall of incubator is equipped with control panel, the bottom of blake box is equipped with the universal wheel.
The principle and the effect of the technical scheme of the utility model are as follows:
during the use, move the device to the target location, the switch on, place the blake bottle in the recess, to adding nutrient solution in the blake bottle, material such as tumour cell tissue piece, then start the electric plate according to the demand through control panel, magnetic field generating device and motor, the electric plate provides the temperature environment that suits the growth for tumour cell, magnetic field generating device drives the magnetic rotor telemechanical through magnetic force, stir the material in the blake bottle, make its evenly distributed in the blake bottle, the motor drives drive gear and rotates, thereby make drive gear drive the rotation of the same speed of each blake bottle, and make the blake bottle rotate in-process thermally equivalent.
Compared with the end face gear of the tumor cell culture device in the prior art, the tumor cell culture device can carry out magnetic stirring without manual operation, and can culture a plurality of samples under the same condition, so that the result of a multi-group variable comparison experiment is more accurate, and the influence of objective and human factors on the experiment is greatly reduced.
Further, the culture bottle comprises a rectangular shell, a cover body in sliding insertion connection is arranged on the upper side of the shell, an inclined neck is fixedly communicated with one side of the shell, and an inclined neck cover is arranged at the outer end of the inclined neck.
The culture bottle designed in the way is convenient and practical, the cover body is opened, the tumor cell tissues and the culture solution are placed from the upper part of the shell, and the tumor cell tissues can be placed at intervals and are smeared more uniformly; after the cultivation is finished, when a tumor cell tissue sample is taken, the sample is taken from the inclined neck, so that the contact between the cell tissue and the outside can be reduced, and the pollution is avoided.
Furthermore, a vertical opening is formed in the position, corresponding to the inclined neck of the culture bottle, of the clamping groove, and a fixing rod is arranged at the upper end of the clamping groove.
The draw-in groove can carry out the block to the blake bottle like this, and the torticollis can be placed to vertical opening, and makes things convenient for getting of blake bottle to put, and the dead lever can be in the same place blake bottle and draw-in groove zonulae occludens, has fully guaranteed stability.
Furthermore, the outer side of the sealing cover is provided with an observation window corresponding to the mounting column.
Therefore, the culture condition of cells can be directly checked through the observation window, and the opening times of the sealing cover are reduced, so that the interference of heat dissipation and other external factors on cell culture is reduced.
Further, the inner walls of the incubator and the sealing cover are both provided with heat-insulating coatings.
Therefore, the heat in the incubator can be prevented from dissipating, the energy is saved, and the constant temperature effect is better achieved.
Drawings
FIG. 1 is a schematic structural diagram of a tumor cell culture apparatus according to the present invention;
FIG. 2 is a top view of a tumor cell culture apparatus according to the present invention;
FIG. 3 is a schematic structural diagram of a culture flask of the tumor cell culture apparatus according to the present invention;
FIG. 4 is a left side view of a culture flask of the tumor cell culture apparatus of the present invention;
the reference numbers in the figures are as follows: incubator 1, blake bottle 2, casing 21, lid 22, torticollis 23, torticollis lid 24, magnetic field generating device 3, backup pad 4, round hole 5, erection column 6, draw-in groove 61, sawtooth 62, vertical opening 63, dead lever 64, motor 7, protective housing 8, electric plate 9, pivot 10, drive gear 11, sealed lid 12, observation window 13, universal wheel 14.
Detailed Description
The utility model is described in further detail below with reference to the following figures and detailed description:
as shown in fig. 1 and fig. 2, a tumor cell culture device comprises an incubator 1 and a culture bottle 2, a magnetic field generating device 3 is arranged at the bottom of an inner cavity of the incubator 1, a support plate 4 is fixedly connected above the magnetic field generating device 3, six circular holes 5 arranged in a circle are formed in the support plate 4 in the embodiment, a mounting column 6 is rotatably connected in the circular holes 5, a clamping groove 61 is formed in the upper end surface of the mounting column 6, as shown in fig. 3 and fig. 4, the culture bottle 2 comprises a rectangular shell 21, a cover 22 inserted and slid is arranged on the upper side of the shell 21, an inclined neck 23 is fixedly communicated with the left side of the shell 21, an inclined neck cover 24 is arranged at the outer end of the inclined neck 23, a magnetic rotor is arranged in the culture bottle 2, the culture bottle 2 is arranged in the clamping groove 61, the clamping groove 61 is as large as the culture bottle 2, a vertical opening 63 is arranged at the position of the clamping groove 61 corresponding to the inclined neck of the culture bottle 2, a fixing rod 64 is arranged at the upper end of the clamping groove 61, the front end of a fixed rod 64 is rotationally connected with a clamping groove 61, the rear end of the fixed rod 64 is clamped with the clamping groove 61, the outer side of the bottom of a mounting column 6 is provided with saw teeth 62, the center of a supporting plate 4 is provided with a motor 7, the outer part of the motor 7 is sleeved with a protective shell 8, the surface of the protective shell 8 is provided with an electric heating plate 9, the lower end of the motor 7 is provided with a rotating shaft 10, the lower end of the rotating shaft 10 is provided with a driving gear 11, the driving gear 11 is positioned between a magnetic field generating device 3 and the supporting plate 4, the driving gear 11 is meshed with the saw teeth 62 on the mounting column 6, the top of the incubator 1 is provided with a sealing cover 12 which is rotationally connected, the outer side of the sealing cover 12 is provided with an observation window 13 corresponding to the mounting column 6, the observation window 13 is made of transparent materials, the inner walls of the incubator 1 and the sealing cover 12 are both provided with heat preservation coatings, the outer wall of the incubator 1 is provided with a control panel, and the control panel is in circuit connection with the magnetic field generating device 3, the motor 7 and the electric heating plate 8, the bottom of the incubator 1 is provided with universal wheels 14 with braking means.
When in use, the culture device is moved to a target position through the universal wheel 14 at the bottom of the culture device, the power is switched on, then the sealing cover 12 is opened, the culture bottle 2 is placed in the groove 61, the cover body 22 is taken down, a proper amount of rat tail collagen is dripped into the culture bottle 2 through the opening at the upper end of the shell 21, then the cover body 22 is closed, the fixing rod 64 is rotated to fix the culture bottle 2, the magnetic field generating device 3 is started through the control panel, the magnetic rotor is driven by the magnetic field generated by the magnetic field generating device 3 to rotate, so that the rat tail collagen is uniformly coated in the shell 21, then the cover body 22 is opened, tumor cell tissue blocks are placed in the shell 21 at intervals, nutrient solution is added, then the shell 21 is covered, the fixing rod 64 is used for fixing, the sealing cover 12 is closed, then the control panel is regulated and set, the electric heating plate 9, the magnetic field generating device 3 and the motor 7 are started as required, magnetic field generating device 3 drives the magnetic rotor telemechanical through magnetic force, stirs the material in the blake bottle 2, makes its evenly distributed in blake bottle 2, and motor 7 drives drive gear 11 and rotates, and drive gear 11 drives the sawtooth 62 rotation of 6 bottoms of erection column to make the rotation of the same speed of each blake bottle 2, after the tumour cell culture finishes, take out blake bottle 2, take off torticollis lid 24, from the opening part sample of torticollis 23.
The foregoing is merely an example of the present invention and common general knowledge of known specific structures and features of the embodiments is not described herein in any greater detail. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (5)
1. The utility model provides a tumour cell culture apparatus, includes incubator (1) and blake bottle (2), its characterized in that: the magnetic field generator (3) is arranged at the lower part of the incubator (1), a supporting plate (4) is arranged above the magnetic field generator (3), a plurality of circular holes (5) distributed in a circumferential mode are formed in the supporting plate (4), an installation column (6) is connected in the circular holes (5) in a rotating mode, a clamping groove (61) is formed in the upper end of the installation column (6), the culture bottle (2) is placed in the clamping groove (61), a magnetic rotor is placed in the culture bottle (2), sawteeth (62) are arranged on the outer side of the bottom of the installation column (6), a motor (7) is arranged at the center of the supporting plate (4), a protective shell (8) is sleeved outside the motor (7), an electric heating plate (9) is arranged on the surface of the protective shell (8), a rotating shaft (10) is arranged at the lower end of the motor (7), and a driving gear (11) is arranged at the lower end of the rotating shaft (10), drive gear (11) are located between magnetic field generating device (3) and backup pad (4), drive gear (11) and sawtooth (62) mesh mutually, the top of incubator (1) is equipped with sealed lid (12) of rotation connection, the outer wall of incubator (1) is equipped with control panel, the bottom of incubator (1) is equipped with universal wheel (14).
2. A tumor cell culture apparatus according to claim 1, wherein: the culture bottle (2) comprises a rectangular shell (21), a cover body (22) which is inserted in a sliding mode is arranged on the upper side of the shell (21), an inclined neck (23) is fixedly communicated with one side of the shell (21), and an inclined neck cover (24) is arranged at the outer end of the inclined neck (23).
3. A tumor cell culture apparatus according to claim 1, wherein: the position of the clamping groove (61) corresponding to the inclined neck (23) of the culture bottle (2) is provided with a vertical opening (63), and the upper end of the clamping groove (61) is provided with a fixing rod (64).
4. A tumor cell culture apparatus according to claim 1, wherein: and an observation window (13) corresponding to the mounting column (6) is arranged on the outer side of the sealing cover (12).
5. A tumor cell culture apparatus according to claim 1, wherein: and heat-insulating coatings are arranged on the inner walls of the incubator (1) and the sealing cover (12).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022589016.4U CN215404280U (en) | 2020-11-11 | 2020-11-11 | Tumor cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022589016.4U CN215404280U (en) | 2020-11-11 | 2020-11-11 | Tumor cell culture device |
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| Publication Number | Publication Date |
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| CN215404280U true CN215404280U (en) | 2022-01-04 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022589016.4U Active CN215404280U (en) | 2020-11-11 | 2020-11-11 | Tumor cell culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115992096A (en) * | 2023-02-22 | 2023-04-21 | 赛尔医学科技(山东)有限公司 | Tumor cell detection method |
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2020
- 2020-11-11 CN CN202022589016.4U patent/CN215404280U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115992096A (en) * | 2023-02-22 | 2023-04-21 | 赛尔医学科技(山东)有限公司 | Tumor cell detection method |
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