CN204356343U - Cell culture bags - Google Patents
Cell culture bags Download PDFInfo
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- CN204356343U CN204356343U CN201520090164.8U CN201520090164U CN204356343U CN 204356343 U CN204356343 U CN 204356343U CN 201520090164 U CN201520090164 U CN 201520090164U CN 204356343 U CN204356343 U CN 204356343U
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- inlet pipe
- culture bags
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Abstract
The utility model discloses a kind of cell culture bags, comprise bag (1), described cell culture bags also comprises the liquid-inlet pipe (2) for inputting enchylema and cell and the liquid flowing tube (3) for extracting enchylema and cell, described liquid-inlet pipe (2) and described liquid flowing tube (3) are sealedly connected on the relative side of described bag (1) respectively, and the mutual remotely interval of the fluid port of the feed liquor port of described liquid flowing tube (3) and described liquid-inlet pipe (2) is arranged.The cell culture bags provided due to the utility model is except having liquid-inlet pipe, also be provided with independently liquid flowing tube, and liquid flowing tube and liquid-inlet pipe port mutually away from interval arrange, therefore, feed liquor and the process of getting liquid can be carried out relatively independently, avoid mutual pollution.
Description
Technical field
The utility model relates to cell culture apparatus field, particularly, relates to a kind of cell culture bags.
Background technology
Cell culture technology, also known as cell clone technology, can maintain cells survival by cell cultures, realizes cell proliferation and products of cellular metabolism after obtaining cell proliferation.Cell cultures has now been widely used in the every field such as biology, medical science, new drug development, becomes technology basic, the most most crucial in biotechnology.
The culture apparatus that cell cultures is commonly used comprises Tissue Culture Dish, Tissue Culture Flask.But, in the process of carrying out fairly large cell cultures (obtaining products of cellular metabolism as amplification immunocyte carries out adoptive immunotherapy, extensive amplifying cells packaging virus and extensive amplifying cells), conventional Tissue Culture Dish, Tissue Culture Flask exist that bottleneck opening is comparatively large, stopping property is poor.When internal media is more, easily overflow, cause the probability of cell contamination to increase.And it is large that Tissue Culture Dish, Tissue Culture Flask need to take storage area.Especially for cultivation suspension cell, require high to the Air-cleaning technique in culture vessel, be statically placed in Tissue Culture Dish, Tissue Culture Flask the mode of carrying out cultivating and easily produce cell aggregation, problem that local cell density is large, thus be unfavorable for exchange of air, cause the decline of cell proliferation quantity.
At present, also there is a kind of cell culture bags using the macromolecule material of gas-permeable, liquid-impermeable to make, the exchange of bag increase and outside air can be utilized, and good airproof performance.But this cell culture bags, carrying out feed liquor by liquid-inlet pipe, getting the phenomenon that there will be pollution mutually when liquid operates, reduces culture effect.
Utility model content
The purpose of this utility model is to provide a kind of cell culture bags, and this cell culture bags effectively can be avoided feed liquor and get the mutual pollution in liquid process.
To achieve these goals, the utility model provides a kind of cell culture bags, comprise bag, described cell culture bags also comprises the liquid-inlet pipe for inputting enchylema and cell and the liquid flowing tube for extracting enchylema and cell, described liquid-inlet pipe and described liquid flowing tube are sealedly connected on the relative side of described bag respectively, and the mutual remotely interval of the fluid port of the feed liquor port of described liquid flowing tube and described liquid-inlet pipe is arranged.
Preferably, described bag is rectangular configuration, and described liquid flowing tube is positioned at the following of described rectangular configuration, and described liquid-inlet pipe is positioned at the top of described rectangular configuration.
Preferably, described liquid-inlet pipe is that multiple and along described bag interval, described top is arranged.
Preferably, described liquid-inlet pipe is two, and these two liquid-inlet pipes are arranged symmetrically with about the top mid point of described rectangular configuration, and described liquid flowing tube is one and is positioned on the following mid point of described rectangular configuration.
Preferably, the symmetry centre between described two liquid-inlet pipes is provided with suppending hole.
Preferably, the arc convex around described suppending hole is formed between described two liquid-inlet pipes.
Preferably, the bore of described liquid flowing tube is less than the bore of described liquid-inlet pipe.
Preferably, described bag is rectangular configuration, and the top mid point of this rectangular configuration is provided with suppending hole, and two following corners of this rectangular configuration are formed with fixed orifices.
Preferably, described bag is provided with containing mark line.
Preferably, the outside of described liquid-inlet pipe and described liquid flowing tube is formed with the ring-shaped attachment flange of be tightly connected infusion pipeline or seal cap respectively.
Pass through technique scheme, the cell culture bags provided due to the utility model is except having liquid-inlet pipe, also be provided with independently liquid flowing tube, and liquid flowing tube and liquid-inlet pipe port mutually away from interval arrange, therefore, feed liquor and the process of getting liquid can be carried out relatively independently, avoid mutual pollution.
Other feature and advantage of the present utility model are described in detail in embodiment part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to provide further understanding of the present utility model, and forms a part for specification sheets, is used from explanation the utility model, but does not form restriction of the present utility model with embodiment one below.In the accompanying drawings:
Fig. 1 is the structural representation of the cell culture bags that the utility model preferred implementation provides.
Description of reference numerals
1 bag 2 liquid-inlet pipe
3 liquid flowing tube 4 suppending holes
5 fixed orifices 6 containing mark lines
71 infusion pipeline 72 seal caps
8 ring-shaped attachment flange 9 arc convex
Embodiment
Below in conjunction with accompanying drawing, embodiment of the present utility model is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the utility model, is not limited to the utility model.
In the utility model, when not doing contrary explanation, the noun of locality of use such as " upper and lower " is that the cell culture bags provided at the utility model is in that to hang working order undefined, and " inside and outside " refers to the interior and outer of corresponding component profile.
As shown in Figure 1, the utility model provides a kind of cell culture bags, comprise bag 1, wherein, the bag 1 of this cell culture bags can adopt the macromolecular material of gas-permeable, liquid-impermeable to make, such as EVA (plastic, rubber) material hot synthetic type is made, EVA material is material well known in the art, i.e. ethylene-vinyl acetate copolymer, also known as plastic, rubber, be characterized in that there is good flexibility, elasticity as rubber, high temperature resistant, at-50 DEG C, still there is good pliability, the transparency and surface luster property, its chemical stability is good, anti-aging and resistance to ozone intensity is good, nontoxicity, and molding processibility is good.Further, this materials application is can O thoroughly in the advantage of the bag 1 of cell culture bags
2and CO
2but liquid-tight, and do not have biology, chemically reactive, can ultraviolet-transmitting, visible ray and infrared rays, and bag 1 can keep toughness in-198 DEG C of liquid nitrogen in addition, and carries out autoclaving at 200 DEG C.In addition, in order to the situation of clear display internal liquid, preferably, bag 1 is provided with containing mark line 6, thus accurately controls the feed liquor of enchylema and cell and get liquid and amass, easy to operate.
In order to realize the purpose of this utility model, the cell culture bags that the utility model provides also comprises the liquid-inlet pipe 2 for inputting enchylema and cell and the liquid flowing tube 3 for extracting enchylema and cell, liquid-inlet pipe 2 and liquid flowing tube 3 are sealedly connected on the relative side of bag 1 respectively, and the mutual remotely interval of the fluid port of the feed liquor port of liquid flowing tube 3 and liquid-inlet pipe 2 is arranged.Therefore, disconnect mutually remotely interval by the liquid flowing tube 3 of additional designs and by the fluid of its feed liquor port and liquid-inlet pipe 2 to arrange, can complete independently enchylema and cell feed liquor and get liquid operation, avoid the mutual pollution in two operating process, culture effect is good.
It should be noted that, the embodiment that can realize above-mentioned technical conceive has multiple, the quantity of such as liquid-inlet pipe 2 and liquid flowing tube 3, structure and decoration form etc., the utility model for convenience of description, preferred implementation is wherein introduced at this, this preferred implementation, only for illustration of the utility model, is not limited to the utility model.
As shown in Figure 1, in preferred implementation of the present utility model, bag 1 is rectangular configuration, and like this, conveniently get liquid and feed liquor operation, liquid flowing tube 3 is positioned at the following of rectangular configuration, and liquid-inlet pipe 2 is positioned at the top of rectangular configuration.Like this, the enchylema accumulated in bottom bag 1 and cell can be extracted convenient for taking liquid pipe 3, also facilitate enchylema and cell to enter bag 1 from top simultaneously.It should be noted that; rectangular configuration described in the utility model not only comprises the rectangular configuration of standard; also comprise the substantially rectangular structure with fillet as shown in Figure 1; in addition in other embodiments; bag 1 also can be other shapes; other polygonized structures such as such as circle, triangle, also should drop in protection domain of the present utility model for this type of distortion.
Further, in order to promote feed liquor efficiency, preferably, liquid-inlet pipe 2 is that multiple and along bag 1 interval, top is arranged.Like this except lifting feed liquor efficiency, at least one liquid-inlet pipe 2 wherein can also be made as the use of vapor pipe, also make enchylema and cell enter in bag 1 by other liquid-inlet pipes 2 smoothly, good operability.Wherein as a kind of preferred embodiment, liquid-inlet pipe 2 is two, and these two liquid-inlet pipes 2 are arranged symmetrically with about the top mid point of rectangular configuration, and liquid flowing tube 3 is one and is positioned on the following mid point of rectangular configuration.Thus the structure of the cell culture bags making the utility model provide is the most reasonable, be easy to processing.In other embodiments, liquid-inlet pipe 2 and liquid flowing tube 3 also have the distortion of various quantity and arranged direction, and these distortion all should drop in protection domain of the present utility model.
In addition, more preferably, the symmetry centre between two liquid-inlet pipes 2 is provided with suppending hole 4, thus suspends cell culture bags evenly by this suppending hole 4, facilitates feed liquor, gets liquid and fluid infusion operation, increase gas-liquid exchange rate, make the culture effect of cell better.Further, in order to increase the intensity at suppending hole 4 place, preferably, the arc convex 9 around suppending hole 4 is formed between two liquid-inlet pipes 2.Also can not take space in bag 1 by arranging this arc convex 9, and make liquid-inlet pipe 2 connection and reasonable arrangement of both sides, Stability Analysis of Structures.In addition, in order to ensure the stable suspension of bag 1, two corners that can also arrange following (namely the arranging the limit of liquid flowing tube 3) of the bag 1 being formed as rectangular configuration are formed with fixed orifices 5, bag 1 is fixed by mounting blocks such as tumbler pins, thus by the symmetrical suppending hole 4 of mid point in the top of rectangular configuration and the fixed orifices 5 of following two corners, bag 1 is placed on balance and stability ground.
In addition, in the utility model, conveniently flow is controlled getting in liquid process, preferably, the bore of liquid flowing tube 3 is less than the bore of liquid-inlet pipe 2, and more small-bore liquid flowing tube can conveniently carry out sampling detection by controlling flow, and larger liquid-inlet pipe 2 then can promote the effect of feed liquor.Wherein the ratio of the bore of liquid flowing tube 3 between liquid-inlet pipe 2 can be 1:1.2 ~ 1.8.
In addition, in order to ensure the sealing to the enchylema in bag 1 and cell, first liquid-inlet pipe 2 and liquid flowing tube 3 can be heat sealing at bag 1 respectively in the manufacturing processed of bag 1 edge on to ensure stopping property.In addition preferably, the outside (namely exposing the part of bag 1) of liquid-inlet pipe 2 and liquid flowing tube 3 is all formed by Hermetical connecting structure, to connect infusion pipeline 71 or seal cap 72, wherein infusion pipeline 71 may be used for joint detection peristaltic pump and carries out supplemental medium, also the liquid-inlet pipe that may be used for being vented or be communicated with another cell culture bags is to carry out the cultivation of extended volume, namely can be communicated with multiple cell culture bags by infusion pipeline 71, to carry out relatively large cell cultures simultaneously.Seal cap 72 then can by idle liquid-inlet pipe 2 or liquid flowing tube 3 capping to play sealing function.Particularly, Hermetical connecting structure can be formed as being tightly connected infusion pipeline 71 or the ring-shaped attachment flange 8 of seal cap 72.Like this, the liquid-inlet pipe 2 of (connecting seal cap 72) and the stopping property of liquid flowing tube 3 under (connect infusion pipeline 71) or off working state under can working order being ensured by the male-female engagement of resilient material, avoid enchylema and cell contaminated.
To sum up, namely the utility model can ensure the culture effect of cell, and enchylema and cell can be avoided again not contaminated, and structure is ingenious, has higher practicality and promotional value.
Below preferred implementation of the present utility model is described by reference to the accompanying drawings in detail; but; the utility model is not limited to the detail in above-mentioned embodiment; in technical conceive scope of the present utility model; can carry out multiple simple variant to the technical solution of the utility model, these simple variant all belong to protection domain of the present utility model.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the utility model illustrates no longer separately to various possible array mode.
In addition, also can carry out arbitrary combination between various different embodiment of the present utility model, as long as it is without prejudice to thought of the present utility model, it should be considered as content disclosed in the utility model equally.
Claims (10)
1. a cell culture bags, comprise bag (1), it is characterized in that, described cell culture bags also comprises the liquid-inlet pipe (2) for inputting enchylema and cell and the liquid flowing tube (3) for extracting enchylema and cell, described liquid-inlet pipe (2) and described liquid flowing tube (3) are sealedly connected on the relative side of described bag (1) respectively, and the mutual remotely interval of the fluid port of the feed liquor port of described liquid flowing tube (3) and described liquid-inlet pipe (2) is arranged.
2. cell culture bags according to claim 1, it is characterized in that, described bag (1) is rectangular configuration, and described liquid flowing tube (3) is positioned at the following of described rectangular configuration, and described liquid-inlet pipe (2) is positioned at the top of described rectangular configuration.
3. cell culture bags according to claim 2, is characterized in that, described liquid-inlet pipe (2) is arranged for multiple and along described bag (1) interval, described top.
4. cell culture bags according to claim 3, it is characterized in that, described liquid-inlet pipe (2) is two, these two liquid-inlet pipes (2) are arranged symmetrically with about the top mid point of described rectangular configuration, and described liquid flowing tube (3) is one and is positioned on the following mid point of described rectangular configuration.
5. cell culture bags according to claim 4, is characterized in that, the symmetry centre between described two liquid-inlet pipes (2) is provided with suppending hole (4).
6. cell culture bags according to claim 5, is characterized in that, is formed with the arc convex (9) around described suppending hole (4) between described two liquid-inlet pipes (2).
7. cell culture bags according to claim 1, is characterized in that, the bore of described liquid flowing tube (3) is less than the bore of described liquid-inlet pipe (2).
8. cell culture bags according to claim 1, it is characterized in that, described bag (1) is rectangular configuration, and the top mid point of this rectangular configuration is provided with suppending hole (4), and two following corners of this rectangular configuration are formed with fixed orifices (5).
9. cell culture bags according to claim 1, is characterized in that, described bag (1) is provided with containing mark line (6).
10. cell culture bags according to claim 1, it is characterized in that, the outside of described liquid-inlet pipe (2) and described liquid flowing tube (3) is formed with the ring-shaped attachment flange (8) of the infusion pipeline that is tightly connected (71) or seal cap (72) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520090164.8U CN204356343U (en) | 2015-02-09 | 2015-02-09 | Cell culture bags |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520090164.8U CN204356343U (en) | 2015-02-09 | 2015-02-09 | Cell culture bags |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204356343U true CN204356343U (en) | 2015-05-27 |
Family
ID=53257824
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520090164.8U Active CN204356343U (en) | 2015-02-09 | 2015-02-09 | Cell culture bags |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204356343U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385597A (en) * | 2015-12-23 | 2016-03-09 | 上海吉凯基因科技有限公司 | Cell culture bag and application thereof |
| CN105420104A (en) * | 2015-12-09 | 2016-03-23 | 中国航天员科研训练中心 | Cell culture bag with three-dimensional structure |
| US11136540B2 (en) | 2016-05-20 | 2021-10-05 | Corning Incorporated | Erlenmeyer flask assemblies with liners |
-
2015
- 2015-02-09 CN CN201520090164.8U patent/CN204356343U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420104A (en) * | 2015-12-09 | 2016-03-23 | 中国航天员科研训练中心 | Cell culture bag with three-dimensional structure |
| CN105385597A (en) * | 2015-12-23 | 2016-03-09 | 上海吉凯基因科技有限公司 | Cell culture bag and application thereof |
| US11136540B2 (en) | 2016-05-20 | 2021-10-05 | Corning Incorporated | Erlenmeyer flask assemblies with liners |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP02 | Change in the address of a patent holder | ||
| CP02 | Change in the address of a patent holder |
Address after: 311121 room 402, building 3, No. 1500, Wenyi West Road, Cangqian street, Yuhang District, Hangzhou City, Zhejiang Province Patentee after: HANGZHOU S-EVANS BIOSCIENCES, Ltd. Address before: 311121 No. 25, Fengling Road, Wuchang Street, Yuhang District, Hangzhou City, Zhejiang Province Patentee before: HANGZHOU S-EVANS BIOSCIENCES, Ltd. |