CN105420104A - Cell culture bag with three-dimensional structure - Google Patents
Cell culture bag with three-dimensional structure Download PDFInfo
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- CN105420104A CN105420104A CN201510906678.0A CN201510906678A CN105420104A CN 105420104 A CN105420104 A CN 105420104A CN 201510906678 A CN201510906678 A CN 201510906678A CN 105420104 A CN105420104 A CN 105420104A
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- Prior art keywords
- bag
- cell culture
- sampling
- culture bags
- dimensional arrangement
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to cell culture bag and aims to provide a cell culture bag with a three-dimensional structure, a higher space utilization rate is realized in a closed space, on-line detection sensors for the on-line cell state and cytology index can be mounted by using integrated sensor connectors, and on-line cell sampling is completed by the aid of a sampling bag. The cell culture bag comprises a reagent connector, a bag body, sensor connectors and a sampling bag, wherein the sampling bag is in a three-dimensional structure, and a plurality of sensors can be mounted; the reagent connector is mounted at the top of the bag body; the sensor connectors are mounted on one side of the bottom of the bag body, and the sampling bag is mounted on the other side of the bottom of the bag body. With the adoption of the technical scheme, the cell culture bag with the three-dimensional structure has the distinctive technical characteristics that 1, the sampling bag is in the three-dimensional structure so that the closed culture space can be effectively used; 2, the cell culture bag is equipped with the sampling bag so that on-line sampling can be realized.
Description
Technical field
The present invention relates to one and possesses cell culture bags, is particularly integrated with the three-dimensional arrangement cell culture bags of sensor interface and sampling bag.
Background technology
Current in cytobiology, life science and relevant medical research field, cell cultures requires to adopt cell culture bags sometimes.In current existing cell culture bags product, the cell culture bags of all specifications is flat structure.It is lower that the limitation that this cell culture bags exists is to use time space utilization ratio in enclosed space, and the applied environment that this cell culture bags has higher requirements for space availability ratio is just not too applicable.
Summary of the invention
The object of the invention is the cell culture bags developing a kind of three-dimensional arrangement, both have higher space availability ratio within the enclosed space; Its integrated sensor interface can be utilized again to install with realizing the detecting line sensor of online cell state and cytology index, and utilize sampling bag to complete the sampling of online cell sample.
Concrete technical scheme of the present invention for achieving the above object:
A kind of three-dimensional arrangement cell culture bags, three-dimensional arrangement cell culture bags, comprises reagent joint, bag, sensor connector and sampling bag, and described bag spatially structure, can install multiple sensor; Described reagent jiont treatment is at bag top; Described sensor connector is installed on side bottom bag, and described sampling bag is installed on opposite side bottom bag.
Further, the index quantity that sensor connector quantity can detect as requested increases and decreases.
Further, bag adopts ethylene/vinyl acetate copolymer (EVA) film thermal synthesis cylindrical shape.
Further, bag diameter is 60mm, high 90mm, and volume is 250ml.
Further, bag top reagent joint and sensor connector all adopt the medical self-sealing joint of standard, utilize heat seal mode to be connected to a bag end face, and wherein reagent joint is heat sealing at center, bag top, and sensor connector is heat sealing at side, bag bottom.
Further, sampling bag, by the machine-shaping of ethylene/vinyl acetate copolymer (EVA) film, is heat sealing at bag bottom opposite side.
Further, sampling bag is cylindric, volume 0.5ml.
After the present invention adopts technique scheme, possess following distinct technical characterstic:
1, cell culture bags spatially structure, can effectively utilize closed culture space;
2, cell culture bags is configured with sampling bag, can realize on-line period, both has higher space availability ratio within the enclosed space; Its integrated sensor interface can be utilized again to install with realizing the detecting line sensor of online cell state and cytology index, and utilize sampling bag to complete the sampling of online cell sample.
Above-mentioned explanation is only the general introduction of technical solution of the present invention, in order to better understand technique means of the present invention, and can be implemented according to the content of specification sheets, coordinates accompanying drawing to be described in detail as follows below with preferred embodiment of the present invention.
Accompanying drawing explanation
Fig. 1 is the structural representation of three-dimensional arrangement cell culture bags of the present invention.
In figure:
1. reagent joint; 2. bag; 3. sensor connector; 4. sampling bag.
Embodiment
Below in conjunction with drawings and Examples, the specific embodiment of the present invention is described in further detail.Following examples for illustration of the present invention, but are not used for limiting the scope of the invention.
See Fig. 1, three-dimensional arrangement cell culture bags of the present invention, comprises reagent joint 1, bag 2, sensor connector 3 and sampling bag 4; Bag 2 spatially structure, can install multiple sensor; Reagent joint 1 is arranged on bag top; Sensor connector 3 is installed on side bottom bag 2, and sampling bag 4 is installed on opposite side bottom bag 2.
Bag adopts ethylene/vinyl acetate copolymer (EVA) film thermal synthesis cylindrical shape, and diameter is 60mm, and high 90mm, is about 250ml.Bag top reagent joint and sensor connector all adopt the medical self-sealing joint of standard, heat seal mode is utilized to be connected to a bag end face, wherein 1 reagent joint is heat sealing at center, bag top, 2 sensor connector are heat sealing at side, bag bottom, sampling bag is also by the machine-shaping of ethylene/vinyl acetate copolymer (EVA) film, in cylindric, volume is about 0.5ml, is heat sealing at bag bottom opposite side.
Operating mode:
To be annotated in bag cell culture fluid by reagent joint with syringe, pull out joint automatic-sealed after syringe.The sensor of requirement of experiment is stretched in bag by sensor connector.The cell culture bags filling nutrient solution can empirically require to carry out cell cultures, in cell cultivation process, can carry out nutrient solution sampling on request with sampling bag.
The above is only the preferred embodiment of the present invention; be not limited to the present invention; should be understood that; for those skilled in the art; under the prerequisite not departing from the technology of the present invention principle; can also make some improvement and modification, these improve and modification also should be considered as protection scope of the present invention.
Claims (7)
1. three-dimensional arrangement cell culture bags, comprise reagent joint (1), bag (2), sensor connector (3) and sampling bag (4), it is characterized in that: described bag (2) spatially structure, multiple sensor can be installed; Described reagent joint (1) is arranged on bag (2) top; Described sensor connector (3) is installed on side, bag (2) bottom, and described sampling bag (4) is installed on bag (2) bottom opposite side.
2. three-dimensional arrangement cell culture bags according to claim 1, is characterized in that: bag (2) adopts ethylene/vinyl acetate copolymer film thermal synthesis cylindrical shape.
3. three-dimensional arrangement cell culture bags according to claim 1, it is characterized in that: described reagent joint (1) and sensor connector (3) all adopt the medical self-sealing joint of standard, heat seal mode is utilized to be connected to bag end face, wherein reagent joint (1) is heat sealing at center, bag top, and sensor connector (3) is heat sealing at side, bag bottom.
4. three-dimensional arrangement cell culture bags according to claim 1, is characterized in that: described sampling bag (4), by the machine-shaping of ethylene/vinyl acetate copolymer film, is heat sealing at bag (2) bottom opposite side.
5. three-dimensional arrangement cell culture bags according to claim 1, is characterized in that: described sampling bag (4) in cylindric, volume 0.5ml.
6. three-dimensional arrangement cell culture bags according to claim 1, is characterized in that: described bag (2) diameter is 60mm, high 90mm, and volume is 250ml.
7. three-dimensional arrangement cell culture bags according to claim 1, is characterized in that: the index quantity increase and decrease that described sensor connector (3) quantity can detect as requested.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510906678.0A CN105420104B (en) | 2015-12-09 | 2015-12-09 | Stereochemical structure cell culture bags |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510906678.0A CN105420104B (en) | 2015-12-09 | 2015-12-09 | Stereochemical structure cell culture bags |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105420104A true CN105420104A (en) | 2016-03-23 |
| CN105420104B CN105420104B (en) | 2018-04-10 |
Family
ID=55498648
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510906678.0A Active CN105420104B (en) | 2015-12-09 | 2015-12-09 | Stereochemical structure cell culture bags |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105420104B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202519255U (en) * | 2012-02-29 | 2012-11-07 | 天津美德太平洋科技有限公司 | Cell culture bag |
| CN203923208U (en) * | 2014-06-04 | 2014-11-05 | 奥星制药设备（石家庄）有限公司 | The supporting cell culture system of disposable sterilized cell culture bags |
| CN204198747U (en) * | 2014-10-22 | 2015-03-11 | 天津灏洋华科生物科技有限公司 | A kind of cell culture bags of convenient operation |
| CN204356343U (en) * | 2015-02-09 | 2015-05-27 | 杭州易文赛生物技术有限公司 | Cell culture bags |
-
2015
- 2015-12-09 CN CN201510906678.0A patent/CN105420104B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202519255U (en) * | 2012-02-29 | 2012-11-07 | 天津美德太平洋科技有限公司 | Cell culture bag |
| CN203923208U (en) * | 2014-06-04 | 2014-11-05 | 奥星制药设备（石家庄）有限公司 | The supporting cell culture system of disposable sterilized cell culture bags |
| CN204198747U (en) * | 2014-10-22 | 2015-03-11 | 天津灏洋华科生物科技有限公司 | A kind of cell culture bags of convenient operation |
| CN204356343U (en) * | 2015-02-09 | 2015-05-27 | 杭州易文赛生物技术有限公司 | Cell culture bags |
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| Publication number | Publication date |
|---|---|
| CN105420104B (en) | 2018-04-10 |
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