CN207193299U - One kind culture DC CIK cell blake bottles - Google Patents
One kind culture DC CIK cell blake bottles Download PDFInfo
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- CN207193299U CN207193299U CN201721151199.3U CN201721151199U CN207193299U CN 207193299 U CN207193299 U CN 207193299U CN 201721151199 U CN201721151199 U CN 201721151199U CN 207193299 U CN207193299 U CN 207193299U
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Abstract
One kind culture DC CIK cell blake bottles, bottle cap including bottle and air-permeable, hydrophobic, bottle includes horizontal part and rake, horizontal part is integrally formed with rake, the rake of bottle cap and bottle coordinates, horizontal baffle is provided with bottle, it is bottle horizontal part I and bottle horizontal part II by the horizontal component of bottle, horizontal baffle is fixedly connected with tilt clapboard, tilt clapboard is inclined upwardly and it has interval between one end of bottle cap and the bottom of bottle cap, and this interval forms the hybrid channel that bottle horizontal part I connects with bottle horizontal part II.The apparatus structure is simple, it is easy to operate, the horizontal component of bottle is bottle horizontal part I and bottle horizontal part II by horizontal baffle, it is respectively used to cultivate DC cells and CIK cell, tilt clapboard can avoid the cell culture fluid of bottle horizontal part I when in use from flowing out, and hybrid channel connects horizontal part I with bottle horizontal part II, after being easy to DC cells and CIK cell culture maturation, culture is blended together, avoids repeatedly changing blake bottle.
Description
Technical field
It the utility model is related to the technical field of cell culture, and in particular to one kind culture DC-CIK cell blake bottles.
Background technology
BMDC（That is DC cells）Energy identifying processing leukaemia, and the coherent signal transmission of leukaemia
To CIK cell, CIK cell is set specially to kill leukaemia, DC-CIK cell therapy leukaemia is the treatment of cell biological therapy
The milestone of leukaemia, therefore external DC-CIK Coculture technologies are arisen at the historic moment.The side of culture DC-CIK cells at present
Case is separately to cultivate DC cells and CIK cell with multiple blake bottles, finally will cultivate ripe DC cells again and CIK cell mixes
Culture, intermediate demand repeatedly change blake bottle, and process is comparatively laborious, and easily causes cell contamination.
Utility model content
The purpose of this utility model is：A kind of culture DC-CIK cell blake bottles, simple in construction, operation side are provided
Just, DC cells and CIK cell can be cultivated simultaneously, avoid repeatedly changing blake bottle.
The technical scheme that the utility model is taken is：
One kind culture DC-CIK cell blake bottles, including the bottle cap of bottle and air-permeable, hydrophobic, bottle include horizontal part and
Rake, horizontal part are integrally formed with rake, and the rake of bottle cap and bottle coordinates, and horizontal baffle is provided with bottle, will
The horizontal component of bottle is bottle horizontal part I and bottle horizontal part II, and horizontal baffle is fixedly connected with tilt clapboard, tilt clapboard
It is inclined upwardly and it has interval between one end of bottle cap and the bottom of bottle cap, this interval forms bottle horizontal part I and bottle
The hybrid channel that horizontal part II connects.
The rib that some sections are triangle is provided with the top of horizontal baffle.
Horizontal baffle is integrally formed with tilt clapboard.
Air-vent is provided with bottle cap, 0.2 μm -0.22 μm of hydrophobic membrane is provided with bottle cap.
The rake of bottle cap and bottle is coordinated by screw thread.
The utility model is simple in construction, easy to operate, and the horizontal component of bottle is bottle horizontal part I and bottle by horizontal baffle
Body horizontal part II, is respectively used to cultivate DC cells and CIK cell, and tilt clapboard can avoid bottle horizontal part I when in use
Cell culture fluid flows out, and hybrid channel connects horizontal part I with bottle horizontal part II, is easy to DC cells and CIK cell to be trained
After ripe, culture is blended together, avoids repeatedly changing blake bottle；Area at the top of the rib increase horizontal baffle of triangle,
The culture amount of increase culture cell。
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
As shown in figure 1, a kind of culture DC-CIK cell blake bottles, including the bottle cap 3 of bottle and air-permeable, hydrophobic, bottle bag
Horizontal part 1 and rake 2 are included, horizontal part 1 is integrally formed with rake 2, and the rake 2 of bottle cap 3 and bottle coordinates, in bottle
Provided with horizontal baffle 6, the horizontal part 1 of bottle is divided and fixed for bottle horizontal part I 11 and bottle horizontal part II 12, horizontal baffle 6
Tilt clapboard 5 is connected with, tilt clapboard 5 is inclined upwardly and it has interval between one end of bottle cap 3 and the bottom of bottle cap 3,
This interval forms the hybrid channel that bottle horizontal part I 11 connects with bottle horizontal part II 12.
The rib 7 that some sections are triangle, the face at the top of increase horizontal baffle 6 are provided with the top of horizontal baffle 6
Product, the culture amount of increase culture cell, while increase the intensity of dividing plate.
Horizontal baffle 6 is integrally formed with tilt clapboard 5, convenient processing, installation.
Air-vent 31 is provided with bottle cap 3,0.2 μm -0.22 μm of hydrophobic membrane 4 is provided with bottle cap 3, can provide and continue
And sterile gas exchanges, while the risk of pollution can be reduced to greatest extent so that bottle cap 3 can only breathe freely, and nutrient solution can not lead to
Cross.
The rake 2 of bottle cap 3 and bottle is coordinated by screw thread, easy to use.
The utility model is simple in construction, easy to operate, when in use, adds nutrient solution to be placed in the mononuclearcell separated
Cultivated in bottle horizontal part I 11（CIK cell and DC cells including original state）, CIK cell is present in culture after 2h
In liquid, DC cells are attached to the bottle wall of bottle horizontal part I 11 and the top of horizontal baffle 6, the culture in bottle horizontal part I 11
Liquid pours into bottle horizontal part II 12 and cultivates CIK cell, is used for cultivating DC cells in bottle horizontal part I 11 plus nutrient solution, treats
After DC cell maturations, DC cells are present with nutrient solution, nutrient solution is poured into bottle horizontal part II 12, DC cells and CIK
Mixing with cells, cultivates DC-CIK cells, above-mentioned to keep when topple over bottle cap 3 to be in hanging tight state.So avoid frequently
Change bottle caused to pollute, save consumptive material again.
Above-described embodiment be to explanation of the present utility model, be not to restriction of the present utility model, it is any new to this practicality
Scheme after type simple transformation belongs to the scope of protection of the utility model.
Claims (5)
1. one kind culture DC-CIK cell blake bottles, including the bottle cap of bottle and air-permeable, hydrophobic, bottle include horizontal part and inclined
Inclined portion, horizontal part are integrally formed with rake, and the rake of bottle cap and bottle coordinates, it is characterised in that：Water is provided with bottle
Flat partition board, be bottle horizontal part I and bottle horizontal part II by the horizontal component of bottle, horizontal baffle be fixedly connected with inclination every
Plate, tilt clapboard is inclined upwardly and it has interval between one end of bottle cap and the bottom of bottle cap, and this interval forms bottle water
The hybrid channel that flat portion I connects with bottle horizontal part II.
2. culture DC-CIK cell blake bottles as claimed in claim 1, it is characterised in that：It is provided with the top of horizontal baffle
Some sections are the rib of triangle.
3. culture DC-CIK cell blake bottles as claimed in claim 1, it is characterised in that：Horizontal baffle and tilt clapboard one
It is body formed.
4. culture DC-CIK cell blake bottles as claimed in claim 1, it is characterised in that：Air-vent is provided with bottle cap,
0.2 μm -0.22 μm of hydrophobic membrane is provided with bottle cap.
5. culture DC-CIK cell blake bottles as claimed in claim 4, it is characterised in that：The rake of bottle cap and bottle leads to
Cross screw thread cooperation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721151199.3U CN207193299U (en) | 2017-09-09 | 2017-09-09 | One kind culture DC CIK cell blake bottles |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721151199.3U CN207193299U (en) | 2017-09-09 | 2017-09-09 | One kind culture DC CIK cell blake bottles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207193299U true CN207193299U (en) | 2018-04-06 |
Family
ID=61792021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721151199.3U Active CN207193299U (en) | 2017-09-09 | 2017-09-09 | One kind culture DC CIK cell blake bottles |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207193299U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943485A (en) * | 2019-04-11 | 2019-06-28 | 广州赛泊泰生物技术有限公司 | Culture bacterium/cell shaking flask of active swap air |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
-
2017
- 2017-09-09 CN CN201721151199.3U patent/CN207193299U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943485A (en) * | 2019-04-11 | 2019-06-28 | 广州赛泊泰生物技术有限公司 | Culture bacterium/cell shaking flask of active swap air |
| CN109943485B (en) * | 2019-04-11 | 2023-11-21 | 江苏谱新生物医药有限公司 | Shaking bottle for actively exchanging air for culturing bacteria/cells |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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