CN111548938A - Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture - Google Patents
Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture Download PDFInfo
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- CN111548938A CN111548938A CN202010515547.0A CN202010515547A CN111548938A CN 111548938 A CN111548938 A CN 111548938A CN 202010515547 A CN202010515547 A CN 202010515547A CN 111548938 A CN111548938 A CN 111548938A
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- 230000001464 adherent effect Effects 0.000 title claims abstract description 44
- 239000000725 suspension Substances 0.000 title claims abstract description 40
- 238000004115 adherent culture Methods 0.000 claims abstract description 39
- 239000012528 membrane Substances 0.000 claims abstract description 10
- 238000004113 cell culture Methods 0.000 claims abstract description 9
- 238000009423 ventilation Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 5
- 239000003292 glue Substances 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 description 55
- 238000000034 method Methods 0.000 description 9
- 238000002659 cell therapy Methods 0.000 description 2
- 210000004405 cytokine-induced killer cell Anatomy 0.000 description 2
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 230000007195 antigen-presenting effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002648 combination therapy Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003631 expected effect Effects 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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Abstract
The invention discloses a novel high-performance culture bottle applicable to suspension and adherent cell mixed culture, which comprises a culture bottle body, wherein the culture bottle body comprises an upper culture bottle shell and an adherent cell culture plate, the upper culture bottle shell covers the top of the adherent cell culture plate, an upper breathable film is arranged at the center of the top wall of the upper culture bottle shell, one side of the upper culture bottle shell is provided with a bottle opening, the bottle opening is provided with a bottle cap, four corners at the top end of the adherent cell culture plate are provided with internal fixing pillars, the adherent cell culture plate is provided with a plurality of bottom breathable grooves arranged along the direction of the bottle opening, and the bottom of the adherent cell culture plate is provided with the bottom breathable film; adopt two-sided ventilative membrane body structure, and the structure of bottom wainscot air channel, improve the mass transfer efficiency among the cell culture process greatly, effectively improve cultivation efficiency.
Description
Technical Field
The invention relates to the technical field of cell culture bottles, in particular to a novel high-performance culture bottle suitable for suspension and adherent cell mixed culture.
Background
Various types of cell therapy drugs enter a high-speed development phase. Some types of cell therapy require multiple cells to be used in concert to produce the desired potency, such as widely used DC-CIK therapy, where CIK (suspension cells) is used as the actual killing potency and immunopotency cells, and DC (adherent cells) are used as antigen presenting cells to direct CIK cells to produce the desired potency. Suspension cells have high culture density and total volume, require higher flux of mass transfer (gas) exchange in the culture environment, and adherent cells require a smooth adherent surface for stable growth. In the traditional process, suspension cells and adherent cells usually adopt different culture bottles to meet the requirement of irregular culture, the operation is complex and tedious in the process, and simultaneously expected effects such as co-excitation among different cells cannot be effectively generated. For example, in CIK, the DC cells have weak antigen presenting effect on CIK cells in the early stage of the process of flask culture, and the efficiency of cell combination is reduced. The multi-cell combination therapy, especially the mixed product of suspension cells and adherent cells, requires a high-efficiency culture consumable with simple operation.
The main purposes of the invention are as follows:
1. realizing high-efficiency culture of suspension cells and adherent cells in mixed cell products;
2. the operation complexity in the process of mixing the suspension cells and the adherent cells is simplified;
3. improve the efficiency of intercellular co-excitation during mixed culture.
Disclosure of Invention
The invention aims to provide a novel high-performance culture bottle suitable for mixed culture of suspension and adherent cells, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme: the utility model provides a novel high performance blake bottle that applicable suspension and adherent cell mixed culture, includes the blake bottle body, the blake bottle body includes blake bottle casing and adherent cell culture board, it covers at the top of adherent cell culture board to go up the blake bottle casing, the roof center department of going up the blake bottle casing is equipped with upper portion ventilated membrane, one side of going up the blake bottle casing is equipped with the bottleneck, be equipped with the bottle lid on the bottleneck, four corners on adherent cell culture board top are equipped with the internal fixation pillar, be equipped with the ventilative groove in bottom that a plurality of set up along the bottleneck direction on the adherent cell culture board, adherent cell culture board bottom is equipped with the bottom ventilated membrane.
Furthermore, the bottle mouth is in threaded connection with the bottle cap, anti-skidding lines are arranged on the outer circumferential wall of the bottle cap, and a sealing gasket is arranged between the bottle mouth and the bottle cap.
Furthermore, the four inner fixing pillars are fixedly connected with the adherent cell culture plate.
Furthermore, the adherent cell culture plate and the bottom ventilation groove are integrally formed, the bottom ventilation groove is rectangular, and the shorter side of the bottom ventilation groove is arc-shaped.
Further, the bottom breathable film is bonded with the adherent cell culture plate through glue.
Furthermore, one side of the upper culture shell, which is far away from the bottle opening, is provided with three raised lines which are uniformly distributed and vertically arranged.
Furthermore, the upper breathable film is circular, and the upper breathable film is embedded in the top wall of the upper culture bottle shell.
Furthermore, the internal fixing support posts are attached to four corners of the inner wall of the upper culture bottle shell.
Furthermore, the top of the adherent cell culture plate is an adherent cell culture plane.
The invention has the beneficial effects that:
1. realizing high-efficiency culture of suspension cells and adherent cells in mixed cell products;
2. the operation complexity in the process of mixing the suspension cells and the adherent cells is simplified;
3. improve the efficiency of intercellular co-excitation during mixed culture.
In conclusion, the invention greatly simplifies the culture operation process of mixed cells (adherent cells and suspension), and solves the problem of complexity of the traditional multi-culture bottle; adopt two-sided ventilative membrane body structure, and the structure of bottom wainscot air channel, improve the mass transfer efficiency among the cell culture process greatly, effectively improve cultivation efficiency.
Drawings
FIG. 1 is a front perspective view of a high performance culture flask of the present invention that is suitable for mixed culture of suspension and adherent cells;
FIG. 2 is a perspective view of an adherent cell culture plate of the novel high-performance culture flask applicable to mixed culture of suspension and adherent cells;
FIG. 3 is a reverse perspective view of a high performance culture flask of the present invention that is suitable for the mixed culture of suspension and adherent cells.
The reference numerals are explained below:
1. a culture bottle body; 11. an upper flask housing; 111. an upper breathable membrane; 112. a convex strip; 12. adherent cell culture plates; 121. a bottom ventilation groove; 122. an inner fixed strut; 123. a bottom gas permeable membrane; 13. a bottle mouth; 131. and (7) a bottle cap.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Several embodiments of the invention are presented in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-3, the present invention provides a technical solution:
novel high-performance culture bottle suitable for suspension and adherent cell mixed culture, comprising a culture bottle body 1, wherein the culture bottle body 1 comprises an upper culture bottle shell 11 and an adherent cell culture plate 12, the upper culture bottle shell 11 covers the top of the adherent cell culture plate 12, an upper breathable film 111 is arranged at the center of the top wall of the upper culture bottle shell 11, a bottle mouth 13 is arranged on one side of the upper culture bottle shell 11, a bottle cap 131 is arranged on the bottle mouth 13, inner fixing pillars 121 are arranged at four corners at the top end of the adherent cell culture plate 12, a plurality of bottom breathable grooves 121 arranged along the direction of the bottle mouth 13 are arranged on the adherent cell culture plate 12, a bottom breathable film 123 is arranged at the bottom of the adherent cell culture plate 12, the mass transfer performance of a cell culture solution in the bottle is improved through the upper breathable film 111, the cell culture efficiency is effectively improved, and the culture requirement of adherent cells is met by, by arranging the bottom air-permeable membrane 123, the mass transfer performance of the cell culture solution in the bottle is improved, the cell culture efficiency is effectively improved, and the high growth rate of suspended cells is still maintained when the suspended cells sink to the bottom;
in order to further improve the use function of the novel high-performance culture bottle suitable for suspension and adherent cell mixed culture, the bottle mouth 13 is in threaded connection with the bottle cap 131, anti-skidding lines are arranged on the outer circumferential wall of the bottle cap 131, a sealing gasket is arranged between the bottle mouth 13 and the bottle cap 131, the bottle cap 131 with the sealing gasket guarantees strict sealing performance of the culture bottle body 1, and strict sterility of the culture bottle body 1 is guaranteed.
In order to further improve the use function of the novel high-performance culture bottle applicable to the mixed culture of suspension and adherent cells, the four internal fixing struts 122 are fixedly connected with the adherent cell culture plate 12.
In order to further improve the novel service function that can be applicable to suspension and adherent cell mixed culture's high performance blake bottle, adherent cell culture plate 12 and bottom ventilative groove 121 integrated into one piece, and bottom ventilative groove 121 is the rectangle, and the shorter limit of bottom ventilative groove 121 is convex, bottom ventilative groove 121: used for the adherent and settled suspension cells at the bottom of the bottle, and provides efficient gas exchange.
In order to further improve the use function of the novel high-performance culture bottle applicable to the mixed culture of suspension and adherent cells, the bottom breathable film 123 is adhered to the adherent cell culture plate 12 through glue.
In order to further improve the service function of the novel high-performance culture bottle applicable to suspension and adherent cell mixed culture, one side of the upper culture shell, which is far away from the bottle mouth 13, is provided with three raised lines 112 which are uniformly distributed and vertically arranged.
In order to further improve the use function of the novel high-performance culture bottle applicable to suspension and adherent cell mixed culture, the upper breathable film 111 is circular, and the upper breathable film 111 is embedded in the top wall of the upper culture bottle shell 11.
In order to further improve the service function of the novel high-performance culture bottle applicable to suspension and adherent cell mixed culture, the internal fixation support column 121 is attached to four corners of the inner wall of the upper culture bottle shell 11, and the fixed position of the adherent cell plate 12 in the bottle body is kept by arranging the internal fixation support column 121 in the culture bottle body 1 on the adherent cell culture plate 12.
In order to further improve the use function of the novel high-performance culture bottle applicable to the mixed culture of suspension and adherent cells, the top of the adherent cell culture plate 12 is an adherent cell culture plane. And the adherent cell culture plane is used for adsorbing the adherent cells so as to achieve high-efficiency growth efficiency.
The invention comprises the following steps:
1. realizing high-efficiency culture of suspension cells and adherent cells in mixed cell products;
2. the operation complexity in the process of mixing the suspension cells and the adherent cells is simplified;
3. improve the efficiency of intercellular co-excitation during mixed culture.
In conclusion, the invention greatly simplifies the culture operation process of mixed cells (adherent cells and suspension), and solves the problem of complexity of the traditional multi-culture bottle; adopt two-sided ventilative membrane body structure, and the structure of bottom wainscot air channel, improve the mass transfer efficiency among the cell culture process greatly, effectively improve cultivation efficiency.
The operation process comprises the following steps: when in use, the bottle cap 131 is opened, the cells to be cultured are poured into the bottle mouth 13, and the bottle cap 131 is closed.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (9)
1. Novel high performance blake bottle that can be applicable to suspension and adherent cell mixed culture, including blake bottle body (1), its characterized in that: the utility model discloses a wall cell culture plate, including the culture bottle (1) including last culture bottle casing (11) and adherent cell culture plate (12), go up the top that culture bottle casing (11) lid was at adherent cell culture plate (12), the roof center department of going up culture bottle casing (11) is equipped with upper portion ventilated membrane (111), the one side of going up culture bottle casing (11) is equipped with bottleneck (13), be equipped with bottle lid (131) on bottleneck (13), four corners on adherent cell culture plate (12) top are equipped with internal fixation pillar (121), be equipped with bottom ventilated groove (121) that a plurality of set up along bottleneck (13) direction on adherent cell culture plate (12), adherent cell culture plate (12) bottom is equipped with bottom ventilated membrane (123).
2. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the bottle mouth (13) is in threaded connection with the bottle cap (131), anti-slip lines are arranged on the outer circumferential wall of the bottle cap (131), and a sealing gasket is arranged between the bottle mouth (13) and the bottle cap (131).
3. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the four inner fixing struts (122) are fixedly connected with the adherent cell culture plate (12).
4. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the adherent cell culture plate (12) and the bottom ventilation groove (121) are integrally formed, the bottom ventilation groove (121) is rectangular, and the shorter side of the bottom ventilation groove (121) is arc-shaped.
5. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the bottom breathable film (123) is bonded with the adherent cell culture plate (12) through glue.
6. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: one side of the upper culture shell far away from the bottle mouth (13) is provided with three raised lines (112) which are uniformly distributed and vertically arranged.
7. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the upper breathable film (111) is circular, and the upper breathable film (111) is embedded in the top wall of the upper culture bottle shell (11).
8. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the inner fixing support columns (121) are attached to four corners of the inner wall of the upper culture bottle shell (11).
9. The novel high-performance culture flask applicable to mixed culture of suspension and adherent cells according to claim 1, wherein: the top of the adherent cell culture plate (12) is an adherent cell culture plane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010515547.0A CN111548938A (en) | 2020-06-09 | 2020-06-09 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010515547.0A CN111548938A (en) | 2020-06-09 | 2020-06-09 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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| Publication Number | Publication Date |
|---|---|
| CN111548938A true CN111548938A (en) | 2020-08-18 |
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| CN202010515547.0A Pending CN111548938A (en) | 2020-06-09 | 2020-06-09 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112410217A (en) * | 2020-11-06 | 2021-02-26 | 英诺维尔智能科技（苏州）有限公司 | Centrifugal culture bottle |
| CN113061521A (en) * | 2021-04-06 | 2021-07-02 | 英诺维尔智能科技（苏州）有限公司 | High-effect cell culture tube |
| CN113462570A (en) * | 2021-07-12 | 2021-10-01 | 英诺维尔智能科技（苏州）有限公司 | High-capacity suspension cell culture bottle |
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| CN109609379A (en) * | 2019-01-21 | 2019-04-12 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel bilateral vapor-permeable type culture bottle |
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2020
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| WO2002042419A2 (en) * | 2000-11-21 | 2002-05-30 | Biocrystal, Ltd. | Cell culture apparatus and methods of use |
| CN101381676A (en) * | 2008-10-23 | 2009-03-11 | 上海交通大学 | Cryopreservation in situ apparatus and method for adherent cell three-dimensional culture |
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| CN113462570A (en) * | 2021-07-12 | 2021-10-01 | 英诺维尔智能科技（苏州）有限公司 | High-capacity suspension cell culture bottle |
| CN113462570B (en) * | 2021-07-12 | 2024-02-09 | 英诺维尔智能科技（苏州）有限公司 | High-capacity suspension cell culture bottle |
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Application publication date: 20200818 |