CN113462570B - High-capacity suspension cell culture bottle - Google Patents
High-capacity suspension cell culture bottle Download PDFInfo
- Publication number
- CN113462570B CN113462570B CN202110782370.5A CN202110782370A CN113462570B CN 113462570 B CN113462570 B CN 113462570B CN 202110782370 A CN202110782370 A CN 202110782370A CN 113462570 B CN113462570 B CN 113462570B
- Authority
- CN
- China
- Prior art keywords
- culture
- culture flask
- connecting pipe
- culture bottle
- bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004113 cell culture Methods 0.000 title claims abstract description 16
- 239000000725 suspension Substances 0.000 title claims abstract description 10
- 238000007789 sealing Methods 0.000 claims abstract description 9
- 239000012528 membrane Substances 0.000 claims description 14
- 238000009826 distribution Methods 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 3
- 238000009434 installation Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 13
- 230000008569 process Effects 0.000 abstract description 12
- 230000003068 static effect Effects 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 27
- 239000007788 liquid Substances 0.000 description 16
- 238000004519 manufacturing process Methods 0.000 description 7
- 230000001502 supplementing effect Effects 0.000 description 7
- 239000006228 supernatant Substances 0.000 description 6
- 230000004907 flux Effects 0.000 description 4
- 238000003306 harvesting Methods 0.000 description 4
- 238000005070 sampling Methods 0.000 description 4
- 238000012864 cross contamination Methods 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 210000002865 immune cell Anatomy 0.000 description 3
- 210000001503 joint Anatomy 0.000 description 3
- 238000012258 culturing Methods 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000010008 shearing Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000001890 transfection Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the technical field of biological pharmacy devices, in particular to a high-capacity suspension cell culture bottle which comprises a culture bottle body, wherein an upper breathable film body is arranged in the upper side of the culture bottle body, the base surface of the culture bottle body is provided with breathable film holes distributed circumferentially, a totally-enclosed bottle cap assembly is arranged in the middle of the upper side of the culture bottle body, a lower breathable film body is arranged on the inner wall of the lower side of the culture bottle body, a culture bottle bottom cap is arranged at the bottom of the culture bottle body, the totally-enclosed bottle cap assembly comprises a culture bottle cap, a sealing gasket, an upper connecting pipe, a lower connecting pipe and a joint, the sealing gasket is arranged in the culture bottle cap, and the totally-enclosed bottle cap assembly is provided with the upper connecting pipe, so that high cell culture activity and effective expansion speed are realized, and meanwhile, cell static culture and vibration culture are met, and process flexibility of different cell types are met.
Description
Technical Field
The invention relates to the technical field of biological pharmacy devices, in particular to a high-capacity suspension cell culture bottle.
Background
The preparation of biological medicine is in the rapid development stage at present, can use various blake bottles in the cultured cells, the large capacity blake bottle on the market at present carries out the gas exchange inefficiency at cell culture in-process, can bear the weight of cell density low, growth and transfection equivalent can be lower, need a large amount of blake bottles or blake bags in order to reach the production efficiency target, influence clinical cell production efficiency, traditional blake bottle generally need uncap the operation in operation, be difficult to satisfy new aseptic production's higher standard, need the blake bottle can realize airtight production's ability, traditional blake bottle blake bag generally is fit for static culture, because the structure restriction can form very big shearing force when shaking table culture, the cell can die rapidly under the influence of big shearing force.
There is a need for a high volume suspension cell culture flask that ameliorates the above-described problems.
Disclosure of Invention
The invention aims to provide a high-capacity suspension cell culture flask, which solves the problems in the prior art.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a high-capacity suspension cell culture bottle, includes the blake bottle body, the upside internally mounted of blake bottle body has upper portion ventilated membrane body, the base surface of blake bottle body has been seted up and has been the ventilated membrane hole of circumference distribution, the department installs totally closed bottle lid subassembly in the middle of the upside of blake bottle body, the downside inner wall of blake bottle body is installed the lower part ventilated membrane body, blake bottle bottom is installed to the bottom of blake bottle body.
As a preferable scheme of the invention, the fully-closed bottle cap assembly comprises a culture bottle cap, a sealing gasket, an upper connecting pipe, a lower connecting pipe and a joint, wherein the sealing gasket is arranged in the culture bottle cap, the upper connecting pipe is arranged on the fully-closed bottle cap assembly, the lower end part of the upper connecting pipe is arranged at the lower side of the culture bottle cap, the lower connecting pipe is arranged at the lower side of the culture bottle cap, and the joint is arranged at the upper end part of the upper connecting pipe.
As a preferable scheme of the invention, the diameters of the upper connecting pipe and the lower connecting pipe are the same, the upper connecting pipe and the lower connecting pipe can be selected to be proper in length, size and number according to the requirement, the connector is a closed luer connector, and the connector can be selected to be proper in number according to the requirement.
As a preferable scheme of the invention, the culture bottle body can be optimized into any proper size and shape according to the requirement, the connection mode between the culture bottle body and the fully-closed bottle cap component is threaded connection, and scale marks are etched on the outer wall of the culture bottle body.
As a preferable scheme of the invention, the upper connecting pipe and the lower connecting pipe can be connected with pump bodies in different forms such as peristaltic pumps through joints.
As a preferable scheme of the invention, the bottom cover of the culture bottle is provided with a bottom supporting boss array, and the lower side of the bottom cover of the culture bottle is provided with through holes distributed in a circumference manner.
As a preferable scheme of the invention, the upper breathable film body and the lower breathable film body can be designed to be attached to any proper position according to the requirement, and the upper breathable film body and the lower breathable film body can be designed to be any proper shape and size according to the requirement.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, when the culture bottle body is high in capacity and density, the high activity rate and the effective expansion speed of cell culture are realized, meanwhile, the static culture and the vibration culture of cells are satisfied, the process flexibility of different cell types is satisfied, the gas flux multiple level of the device is increased compared with that of the traditional culture bottle by the upper breathable film body and the lower breathable film body, the ventilation function of the upper surface and the lower surface of the culture bottle body is ensured, the effective growth of suspended cells after sedimentation is satisfied, and the internal and external pressure balance of the bottle body is realized.
2. According to the invention, the upper connecting pipe, the lower connecting pipe and the joint of the fully-closed bottle cap assembly form a closed pipeline, so that the device can realize the complete closed operation of liquid supplementing (changing), sampling and harvesting, meets the requirements of GMP production, simultaneously provides the closed connecting pipe joint, can be in butt joint with a semi-automatic unit and automatic equipment, can directly remove supernatant after the device is placed in a standing way, is convenient for liquid supplementing and liquid changing operation, eliminates the complicated centrifugal liquid changing operation and the like of the traditional culture bottle in the process, is designed as an integral culture bottle, reduces the risk of cross contamination, can realize the mutual replacement of a common bottle cap and the fully-closed connecting pipe bottle cap, can meet the requirements of static culture and vibration culture, has flexible process matching, and can meet the static culture of immune cells.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present invention;
FIG. 2 is a schematic view of the internal structure of the body of the culture flask according to the invention;
fig. 3 is a schematic structural view of the fully-enclosed bottle cap assembly of the present invention.
In the figure: 1. culturing the bottle body; 2. an upper breathable film body; 3. ventilation film holes; 4. a totally-enclosed bottle cap assembly; 5. a lower breathable film body; 6. a bottom cover of the culture bottle; 7. bottle caps of the culture bottles; 8. a sealing gasket; 9. an upper connecting pipe; 10. a lower connecting pipe; 11. and (3) a joint.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art without making any inventive effort based on the embodiments of the present invention are within the scope of protection of the present invention.
In order that the invention may be readily understood, several embodiments of the invention will be described more fully hereinafter with reference to the accompanying drawings, in which, however, the invention may be embodied in many different forms and is not limited to the embodiments described herein, but instead is provided for the purpose of providing a more thorough and complete disclosure of the invention.
It will be understood that when an element is referred to as being "mounted" on another element, it can be directly on the other element or intervening elements may also be present, and when an element is referred to as being "connected" to the other element, it may be directly connected to the other element or intervening elements may also be present, the terms "vertical", "horizontal", "left", "right" and the like are used herein for the purpose of illustration only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in this description of the invention are for the purpose of describing particular embodiments only and are not intended to be limiting of the invention, with the term "and/or" as used herein including any and all combinations of one or more of the associated listed items.
Referring to fig. 1-3, the present invention provides a technical solution:
the utility model provides a high-capacity suspension cell culture bottle, includes culture flask body 1, and upper portion air-permeable membrane body 2 is installed to the upside internally mounted of culture flask body 1, and air-permeable membrane hole 3 that is circumference distribution has been seted up to the base plane of culture flask body 1, and totally closed bottle lid subassembly 4 is installed to department in the middle of the upside of culture flask body 1, and lower part air-permeable membrane body 5 is installed to the downside inner wall of culture flask body 1, and culture flask bottom 6 is installed to the bottom of culture flask body 1.
Referring to fig. 1-3, the fully-closed bottle cap assembly 4 includes a bottle cap 7, a sealing gasket 8, an upper connecting tube 9, a lower connecting tube 10 and a connector 11, the sealing gasket 8 is installed inside the bottle cap 7, the upper connecting tube 9 is installed on the fully-closed bottle cap assembly 4, the lower end of the upper connecting tube 9 is installed below the bottle cap 7, the lower connecting tube 10 is installed at the upper end of the upper connecting tube 9, the connector 11 is installed at the upper end of the upper connecting tube 9, the diameters of the upper connecting tube 9 and the lower connecting tube 10 are the same, the upper connecting tube 9 and the lower connecting tube 10 can be selected to have proper lengths, sizes and numbers according to requirements, the connector 11 can be selected to have proper numbers according to requirements, the upper connecting tube 9 and the lower connecting tube 10 can be connected with pump bodies of different forms such as peristaltic pumps through the connector 11, the upper connecting pipe 9, the lower connecting pipe 10 and the joint 11 of the fully-closed bottle cap assembly 4 form a closed pipeline, so that the device can realize the complete closed operation of liquid supplementing (liquid changing), sampling and harvesting, meets the production requirement of GMP, simultaneously provides a closed connecting pipe joint, can be in butt joint with a semi-automatic unit and automatic equipment, can directly remove supernatant after the device is placed in a standing way, is convenient for liquid supplementing and liquid changing operation, eliminates the complicated centrifugal liquid changing and other operations of the traditional culture bottle in the process, is designed as an integral culture bottle, reduces the cross contamination risk, can realize the mutual replacement of a common bottle cap and a fully-closed connecting pipe bottle cap, can simultaneously meet the requirements of static culture and vibration culture, has flexible process matching, can meet the stationary culture of immune cells.
Referring to fig. 1 and 2, a bottom supporting boss array is provided on a bottom cover 6 of a culture bottle, through holes distributed circumferentially are provided on the lower side of the bottom cover 6 of the culture bottle, the body 1 of the culture bottle can be optimized into liquid volumes with different sizes and different effective expansion speeds according to requirements, 1000ml,2000ml,5000ml or other specifications and shapes, a connection mode between the body 1 of the culture bottle and a totally-enclosed bottle cover assembly 4 is threaded connection, scale marks are etched on the outer wall of the body 1 of the culture bottle, the upper breathable film body 2 and the lower breathable film body 5 can be attached to any suitable positions according to the requirements in a random shape, the upper breathable film body 2 and the lower breathable film body 5 can be designed into any suitable shapes and sizes according to the requirements, the high activity rate and the effective expansion speed of cell culture can be realized when the body 1 of the culture bottle is in large capacity and high density, meanwhile, the process flexibility of different cell types is met, the gas levels of the device are increased compared with that of a traditional culture bottle, the upper breathable film body 2 and the lower breathable film body 5 can be attached to any suitable shape, and the two lower breathable film bodies can have the functions of the same volume as the upper breathable film body 1 and the lower breathable film body are required to have the same volume, and the functions of the cell can be effectively and the cell can be suspended in the cell can be expanded, and the cell can be effectively and the cell can be suspended in the cell can have the cell can be in the cell can have the cell volume.
The working flow of the invention is as follows: placing proper amount of cells and culture medium into the culture flask body 1, screwing the common culture flask cap 7, performing stationary culture or shaking culture in the culture flask, during cell culture, without changing liquid, without adding culture medium again, after a period of time, harvesting grown cells or products in supernatant, and when another application is performed, placing proper amount of cells and culture medium into the culture flask body 1, inserting the upper connecting tube 9 and the lower connecting tube 10 into the culture flask cap 7, sampling at any time through the joint 11 at the end of the upper connecting tube 9, directly discharging supernatant after the cells effectively settle during culture, performing liquid changing and supplementing operation, extracting supernatant through the upper connecting tube 9, the lower connecting tube 10 and the joint 11, extracting cells, performing cell collecting operation without opening the culture flask cap 7, avoiding pollution, suspending cells in culture solution in shaking culture process, reducing gas exchange efficiency formed by direct contact of lower ventilated membrane 5 and cells, compensating whole gas exchange flux by increasing gas exchange flux on upper surface of liquid, effectively guaranteeing total culture amount, culturing bottle body 1 can ensure effective formation of vortex in shaking culture process, upper connecting pipe 9, lower connecting pipe 10 and joint 11 of totally-enclosed bottle cap assembly 4 form enclosed pipeline, making the device realize completely-enclosed operation of liquid supplementing (changing), sampling and harvesting, meeting GMP production requirement, providing enclosed connecting pipe joint, interfacing with semi-automatic unit and automatic equipment, also making the device capable of directly removing supernatant after standing and placing, facilitating liquid supplementing and changing operation, the device has the advantages that complicated operations such as centrifugal liquid exchange and the like in the process of a traditional culture bottle are eliminated, the integral culture bottle design is integrated, the cross contamination risk is reduced, the common bottle cap and the full-closed connecting tube bottle cap can be mutually replaced, meanwhile, static culture and vibration culture can be met, flexible process matching performance is realized, static culture of immune cells can be met, high activity rate and effective expansion speed of cell culture are realized when the culture bottle body 1 is high in capacity and high in density, static culture and vibration culture of cells are met, process flexibility of different cell types is met, the gas flux multiple level of the device is increased compared with that of the traditional culture bottle, the upper breathable film body 2 and the lower breathable film body 5 are guaranteed to have a breathable function, effective growth after sedimentation of suspended cells is met, and internal and external pressure balance of the bottle body is realized.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (2)
1. The utility model provides a high-capacity suspension cell culture bottle, includes blake bottle body (1), its characterized in that: the utility model discloses a culture flask body, including upper portion ventilated membrane body (2) of the inside installation of upside of culture flask body (1), base surface of culture flask body (1) has been seted up and is circumference distribution's ventilated membrane hole (3), the department installs totally closed bottle lid subassembly (4) in the middle of the upside of culture flask body (1), lower part ventilated membrane body (5) are installed to the downside inner wall of culture flask body (1), culture flask bottom (6) are installed to the bottom of culture flask body (1), totally closed bottle lid subassembly (4) include culture flask bottle lid (7), sealing washer (8), upper portion connecting pipe (9), lower part connecting pipe (10) and joint (11), sealing washer (8) are installed to the internally mounted of culture flask bottle lid (7), install upper portion connecting pipe (9) on totally closed bottle lid subassembly (4), the downside tip of upper portion connecting pipe (9) and the downside that is located culture flask lid (7) installs lower part (10), upper portion connecting pipe (11) are installed to the upside tip (9), lower part connecting pipe (10) and the diameter that need be selected by the same, and the tip (10) is big in order to select the diameter of connecting pipe (10) and the tip (11), and connect (11) can select suitable quantity according to the demand, culture flask body (1) can optimize into arbitrary suitable size and shape according to the demand, connected mode between culture flask body (1) and the totally closed bottle lid subassembly (4) is threaded connection, the outer wall sculpture of culture flask body (1) has the scale mark, be provided with bottom support boss array on culture flask bottom (6), and the through-hole that is circumference distribution has been seted up to the downside of culture flask bottom (6), upper portion ventilated membrane body (2) and lower part ventilated membrane body (5) can be according to the arbitrary suitable position of demand design arbitrary shape subsides, and upper portion ventilated membrane body (2) and lower part ventilated membrane body (5) can be according to the demand design arbitrary suitable shape and size.
2. A high volume suspension cell culture flask as claimed in claim 1 wherein: the upper connecting pipe (9) and the lower connecting pipe (10) can be connected with the peristaltic pump through a joint (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110782370.5A CN113462570B (en) | 2021-07-12 | 2021-07-12 | High-capacity suspension cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110782370.5A CN113462570B (en) | 2021-07-12 | 2021-07-12 | High-capacity suspension cell culture bottle |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113462570A CN113462570A (en) | 2021-10-01 |
| CN113462570B true CN113462570B (en) | 2024-02-09 |
Family
ID=77879869
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110782370.5A Active CN113462570B (en) | 2021-07-12 | 2021-07-12 | High-capacity suspension cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113462570B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206843507U (en) * | 2017-06-08 | 2018-01-05 | 北京焕生汇生物技术研究院 | A kind of suspension cell culture device |
| CN207793282U (en) * | 2017-11-15 | 2018-08-31 | 中国航天员科研训练中心 | Culture bottle for the automatic amplification cultivation of cell |
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
| CN112592824A (en) * | 2020-10-21 | 2021-04-02 | 英诺维尔智能科技（苏州）有限公司 | Reaction bottle for cell culture |
| CN112725176A (en) * | 2021-01-18 | 2021-04-30 | 英诺维尔智能科技（苏州）有限公司 | Full-automatic operation method for connecting luer connector with high-performance cell culture bottle |
-
2021
- 2021-07-12 CN CN202110782370.5A patent/CN113462570B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN206843507U (en) * | 2017-06-08 | 2018-01-05 | 北京焕生汇生物技术研究院 | A kind of suspension cell culture device |
| CN207793282U (en) * | 2017-11-15 | 2018-08-31 | 中国航天员科研训练中心 | Culture bottle for the automatic amplification cultivation of cell |
| CN109706077A (en) * | 2019-01-21 | 2019-05-03 | 英诺维尔智能科技（苏州）有限公司 | A kind of Novel breathable culture bottle |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
| CN112592824A (en) * | 2020-10-21 | 2021-04-02 | 英诺维尔智能科技（苏州）有限公司 | Reaction bottle for cell culture |
| CN112725176A (en) * | 2021-01-18 | 2021-04-30 | 英诺维尔智能科技（苏州）有限公司 | Full-automatic operation method for connecting luer connector with high-performance cell culture bottle |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113462570A (en) | 2021-10-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN218811806U (en) | Cell culture bag | |
| CN110835602A (en) | Three-dimensional cell culture bottle capable of realizing continuous perfusion | |
| CN113462570B (en) | High-capacity suspension cell culture bottle | |
| CN207468646U (en) | High efficiency cell culture bottle | |
| CN205045486U (en) | Ampoule injection vacuum chuck device | |
| CN213142019U (en) | Shake bottle and invert liquid and shift lid | |
| CN207560911U (en) | A kind of seedlings nursing plate convenient for culturing and transplanting seedlings | |
| CN203057904U (en) | Simple fermentation device for liquid strain submerged cultivation | |
| CN209508287U (en) | A kind of steril cell culture bottle | |
| CN205501326U (en) | Centrifugal sedimentation formula bioreactor for plant cell continuous culture | |
| CN204981891U (en) | Cell culture changes bottle | |
| CN205115494U (en) | Cell culture bottle | |
| CN215517462U (en) | Multifunctional stem cell culture plate | |
| CN211689106U (en) | Microcarrier cell culture bag | |
| CN217297834U (en) | High-efficient bacteria culture bottle | |
| CN215517362U (en) | Totally closed cell splendid attire cup | |
| CN204939471U (en) | Replacing formula foam collection device | |
| CN206163603U (en) | One -pipe lithium battery electrolyte solutions impregnator | |
| CN214694230U (en) | Cell culture bottle | |
| CN206089707U (en) | Ginseng cell culture device | |
| CN206109421U (en) | Cell network management of ventilating | |
| JP2021521826A (en) | Bioreactor | |
| CN210736790U (en) | A novel shake bottle for adherent cell culture | |
| CN214032534U (en) | Autoimmune cell culture bottle | |
| CN216585026U (en) | Vertical type cell culture bottle with pointed bottom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |