CN206109421U - Cell network management of ventilating - Google Patents
Cell network management of ventilating Download PDFInfo
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- CN206109421U CN206109421U CN201621021167.7U CN201621021167U CN206109421U CN 206109421 U CN206109421 U CN 206109421U CN 201621021167 U CN201621021167 U CN 201621021167U CN 206109421 U CN206109421 U CN 206109421U
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- webmaster
- filter
- cell
- excircle
- mixing drum
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Abstract
The utility model discloses a cell network management of ventilating is located cell culture tank, including the stirring center, the stirring center is in including vertical (mixing) shaft and coaxial coupling the churn of (mixing) shaft bottom, first filter has been cup jointed to the bottom of churn, first filter include with fixed first end plate is cup jointed to the churn bottom first end plate top with the churn cup joints fixed first upper head plate, and connects the outer circumference of first upper head plate with a filter net pipe of the outer circumference of first end plate, the top of first filter be equipped with with the fixed stirring rake of the outer circumference of churn, the footpath centering at stirring center is equipped with the breather pipe, the breather pipe divide into the vertical breather pipe body that runs through the stirring center, and one end is connected the bottom of breather pipe body, one end is upwards followed in addition the bottom surface of first end plate is stretched into the hook portion of first filter bottom.
Description
Technical field
The utility model is related to a kind of cell ventilating net tube of medical device field.
Background technology
The cell culture of mammal is referred under the conditions in vitro of cell, and cell growth is maintained with propagation with nutrient solution
Technology.Microcarrier cell reactor generally includes culture tank and breather pipe.Nutrient solution and cell are held in culture tank, is cell
Growing environment is provided, breather pipe is used to convey gas in culture tank, and cell draws the nutrition in nutrient solution in incubation
With the dissolved oxygen in gas, for the needs of own growth.
The method of current zooblast microcarrier culture ventilation mainly has two kinds.One kind is to add stirring using top layer oxygen supply
Mode carrying out, the shortcoming of this method is：Gas-liquid specific surface is too little, and often oxygen-supplying amount is inadequate for top layer ventilation oxygen-supplying.It is another
The method of kind is to adopt deep ventilation, i.e., the bottom for inserting culture tank using a breather pipe is supplied, the oxygen supply of this method
Amount can meet the needs of cell growth, but cell can be captured during rising due to bubble, therefore when bubble is rising
During when rupturing, rupturing the shearing force for producing can cause cell death by cell drawing crack, so can cause entirely cultivating
Substantial amounts of cell is damaged in cycle.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, there is provided a kind of cell ventilating net tube, and it can be
While meeting bioreactor deep ventilation, infringement is not produced to cell, improve the survival rate of cell, and ensure cell growth
Uniformity.
A kind of technical scheme for realizing above-mentioned purpose is a kind of cell ventilating net tube, in bioreactor, including stirring
Mix center；
The mixing center includes vertical shaft and the coaxially connected mixing drum in the shaft bottom；
The bottom of the mixing drum is socketed with the first filter, and first filter includes being covered with the mixing drum bottom
The first fixed bottom plate is connect, the first upper head plate being fixed by socket with the mixing drum above first bottom plate, and
Connect the excircle of first upper head plate and the first filtration webmaster of the first bottom plate excircle；First screen pack
Particle diameter of the aperture of pipe excircle less than the bioreactor inner cell culture carrier；
The paddle fixed with the mixing drum excircle is provided with above first filter；
The radial center of the mixing center is provided with breather pipe；The breather pipe is divided into vertically through the ventilation of mixing center
Tube body, and the bottom of one end connection snorkel body, bottom surface of the other end upwards from first bottom plate is stretched
Enter the hook part of first filter bottom.
Further, described first filter webmaster by radially from the first outer layer webmaster for inwardly arranging successively outward, first
Middle level webmaster and the first internal layer webmaster are constituted, the first outer layer webmaster using 80～100 μm of aperture screen pack, described first
Middle level webmaster adopts the screen pack in 300～500 μm of aperture, and the first internal layer webmaster is by thickness 1mm, uniform 10 × 10mm square holes
Steel plate curling form.
Further, the second filter being also socketed with the mixing drum above the paddle, described second
Filter includes the second bottom plate being fixed by socket with the mixing drum and the second upper head plate, and connects second upper head plate
Excircle and the second bottom plate excircle second filtration webmaster；The top surface of second upper head plate is less than the cell
The liquid level of culture tank inner cell nutrient solution；Described second aperture for filtering webmaster excircle is less than the bioreactor inner cell
The particle diameter of culture carrier.
Further, aperture of the aperture of the second filter excircle less than the first filter excircle.
Further, described second filter webmaster by radially from the second outer layer webmaster for inwardly arranging successively outward, the
Two middle level webmasters and the second internal layer webmaster are constituted, and the second outer layer webmaster adopts the screen pack in 150～160 μm of aperture, described
Second middle level webmaster adopts the screen pack in 300～500 μm of aperture, and the second internal layer webmaster is by thickness 1mm, uniform 10 × 10mm
The steel plate curling of square hole is formed.
Further, connected by some communicating pipes between first filter and second filter.
Further, the communicating pipe and the paddle are uniform around the circle alternate of the mixing drum.
A kind of cell ventilating net tube of the present utility model is employed, in bioreactor, including mixing center；It is described
Mixing center includes vertical shaft and the coaxially connected mixing drum in the shaft bottom；The bottom of the mixing drum
The first filter is socketed with, first filter includes the first bottom plate being fixed by socket with the mixing drum bottom, in institute
The first upper head plate that the first bottom plate top is fixed by socket with the mixing drum is stated, and connects the cylindrical of first upper head plate
First filtration webmaster of all and described first bottom plate excircles；Described first aperture for filtering webmaster excircle is less than described thin
The particle diameter of born of the same parents' culture tank inner cell culture carrier；It is provided with above first filter and mixing drum excircle fixation
Paddle；The radial center of the mixing center is provided with breather pipe；The breather pipe is divided into vertically through the ventilation of mixing center
Tube body, and the bottom of one end connection snorkel body, bottom surface of the other end upwards from first bottom plate is stretched
Enter the hook part of first filter bottom.It has the technical effect that：It can while bioreactor deep ventilation is met,
Infringement is not produced to cell, improve the survival rate of cell, and ensure the uniformity of cell growth.
Description of the drawings
Fig. 1 is a kind of structural representation of cell ventilating net tube of the present utility model.
Fig. 2 is a kind of structural representation of the first filtration webmaster of cell ventilating net tube of the present utility model.
Fig. 3 is a kind of structural representation of the second filtration webmaster of cell ventilating net tube of the present utility model.
Fig. 4 is Section A-A schematic diagram in Fig. 1.
Specific embodiment
Refer to Fig. 1, inventor of the present utility model is in order to be able to preferably managing the technical solution of the utility model
Solution, below by specifically embodiment, and combines accompanying drawing and is described in detail：
Refer to Fig. 1, a kind of cell ventilating net tube of the present utility model, including mixing center 1, mixing center be divided into for
The shaft 11 that mixing center 1 is fixed with stirring motor, and it is coaxial with the bottom of shaft 11 in the lower section of shaft
The mixing drum 12 of connection.
The first filter 2 and the second filter 3 are socketed with mixing drum 12, the second filter 3 is located at the first filter 2
Surface.
First filter 2 by the first upper head plate 21, the first bottom plate 22, and connection the first upper head plate 21 excircle and
First filtration webmaster 23 of the excircle of the first bottom plate 22 is constituted, and the first upper head plate 21 and the first bottom plate 22 are and mixing drum
12 are fixed by socket.Wherein the first bottom plate 22 is fixed by socket with the bottom of mixing drum 12.First filter webmaster 23 by radially from
Outer the first outer layer webmaster 231 for inwardly arranging successively, the first middle level webmaster 232 and the first internal layer webmaster 233 are constituted.First outer layer
Webmaster 231 adopts the screen pack in 80～100 μm of aperture, the first middle level webmaster 232 to adopt the screen pack in 300～500 μm of aperture, the
One internal layer webmaster 233 is formed by thickness 1mm, the steel plate curling of uniform 10 × 10mm square holes.
Second filter 3 by the second upper head plate 31, the second bottom plate 32, and connection the second upper head plate 32 excircle and
Second filtration webmaster 23 of the excircle of the second bottom plate 32 is constituted, and the second upper head plate 31 and the second bottom plate 32 are and mixing drum
12 are fixed by socket.Liquid level of the top surface of the second upper head plate 32 less than bioreactor inner cell nutrient solution.Second filters webmaster 33
By radially from 333 groups of the second outer layer webmaster 331 for inwardly arranging successively outward, the second middle level webmaster 332 and the second internal layer webmaster
Into.Second outer layer webmaster 331 adopts the screen pack in 150～160 μm of aperture, the second middle level webmaster 332 to adopt the μ of aperture 300～500
The screen pack of m, the first internal layer webmaster 333 is formed by thickness 1mm, the steel plate curling of uniform 10 × 10mm square holes.
First filters webmaster 23 and second filters the aperture of the excircle of webmaster 33 less than bioreactor inner cell culture load
The particle diameter of body.First filtration webmaster 23 and second filters webmaster 33 all can prevent cell culture vector from blocking using three-decker
First filters webmaster 23 or second filters webmaster 33, and the first of the first filter 2 filters filtration webmaster outside the first of webmaster 23
231 aperture meets cell culture more than the second outer aperture for filtering webmaster 331 of the second filtration webmaster 33 of the second filter 3
The characteristics of carrier suspends in bioreactor.
Above-mentioned design can prevent the cell culture vector in bioreactor from entering the first filter 2 or the second filtration
Device 3, so as to prevent cell cultivation process in loss.
Mixing drum 12 is provided between the first filter 2 and the second filter 3, around the circumference uniform distribution of mixing drum 12
Paddle 13.Such design can strengthen the convection current of cell culture fluid, prevent the damage of cell, improve the survival of cell
Rate.
Connected by some connecting tubes 4 between first filter 2 and the second filter 3, and paddle 13 and communicating pipe 4 are
It is outer uniform around the circle alternate of mixing drum 12.
Meanwhile, the radial center of mixing center 1 is provided with breather pipe 5, and 5 points of breather pipe is for vertically through the logical of mixing center 1
Tracheae body 51, and the bottom of one end connection snorkel body 5, bottom surface of the other end upwards from the first bottom plate 22 is stretched into
The hook part 52 of the bottom of the first filter 2.
Such design, the bubble of ventilation is that, in the implosion of the first filter 2, and cell culture vector is isolated in
Outside one filter 2 and the second filter 3, therefore the rupture of bubble will not cause to damage to the cell on cell culture vector.
Therefore while deep ventilation in bioreactor is looked after, the damage to cell is reduced.Do not break in the first filter 2
The bubble for splitting, also can enter the second filter 3 by communicating pipe 4, and in the implosion of the second filter 3, equally not result in thin
The damage of cell on born of the same parents' culture tank inner cell culture carrier.Bubble oxygen after the first filter 2 or the implosion of the second filter 3
Molecule can filter webmaster 23 or second and filter webmaster 33 by first, support to the cell on cell culture vector.And the first mistake
The design of the filter 3 of filter 2 and second ensure that bioreactor inner cell support uniformity, it is ensured that cell growth it is consistent
Property.
Those of ordinary skill in the art is it should be appreciated that the embodiment of the above is intended merely to illustrate that this practicality is new
Type, and be not used as be to restriction of the present utility model, if in spirit of the present utility model, to the above
Change, the modification of embodiment all will fall in Claims scope of the present utility model.
Claims (7)
1. a kind of cell ventilating net tube, in bioreactor, including mixing center, it is characterised in that：
The mixing center includes vertical shaft and the coaxially connected mixing drum in the shaft bottom；
The bottom of the mixing drum is socketed with the first filter, and first filter includes solid with mixing drum bottom socket
The first fixed bottom plate, the first upper head plate being fixed by socket with the mixing drum above first bottom plate, and connection
First filtration webmaster of the excircle of first upper head plate and the first bottom plate excircle；Described first filters outside webmaster
Particle diameter of the aperture of circumference less than the bioreactor inner cell culture carrier；
The paddle fixed with the mixing drum excircle is provided with above first filter；
The radial center of the mixing center is provided with breather pipe；The breather pipe is divided into vertically through the breather pipe sheet of mixing center
Body, and the bottom of one end connection snorkel body, institute is stretched in bottom surface of the other end upwards from first bottom plate
State the hook part of the first filter bottom.
2. a kind of cell ventilating net tube according to claim 1, it is characterised in that：Described first filters webmaster by radially
From the first outer layer webmaster, the first middle level webmaster and the first internal layer webmaster composition that inwardly arrange successively outward, first outer layer net
Pipe using 80～100 μm of aperture screen pack, first middle level webmaster using 300～500 μm of aperture screen pack, described the
One internal layer webmaster is formed by thickness 1mm, the steel plate curling of uniform 10 × 10mm square holes.
3. a kind of cell ventilating net tube according to claim 1, it is characterised in that：Also it is socketed with the mixing drum and is located at
The second filter above the paddle, second filter includes the second bottom plate being fixed by socket with the mixing drum
With the second upper head plate, and connect the excircle of second upper head plate and the second screen pack of the second bottom plate excircle
Pipe；Liquid level of the top surface of second upper head plate less than the bioreactor inner cell nutrient solution；Described second filters webmaster
Particle diameter of the aperture of excircle less than the bioreactor inner cell culture carrier.
4. a kind of cell ventilating net tube according to claim 3, it is characterised in that：The hole of the second filter excircle
Aperture of the footpath less than the first filter excircle.
5. a kind of cell ventilating net tube according to claim 4, it is characterised in that：Described second filters webmaster by radially
From the second outer layer webmaster, the second middle level webmaster and the second internal layer webmaster composition that inwardly arrange successively outward, second outer layer net
Pipe adopts the screen pack in 150～160 μm of aperture, and second middle level webmaster adopts the screen pack in 300～500 μm of aperture, described
Second internal layer webmaster adopts thickness 1mm, the steel plate curling of uniform 10 × 10mm square holes.
6. a kind of cell ventilating net tube according to claim 3, it is characterised in that：First filter and described second
Connected by some communicating pipes between filter.
7. a kind of cell ventilating net tube according to claim 6, it is characterised in that：The communicating pipe and the paddle are
Circle alternate around the mixing drum is uniform.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621021167.7U CN206109421U (en) | 2016-08-31 | 2016-08-31 | Cell network management of ventilating |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621021167.7U CN206109421U (en) | 2016-08-31 | 2016-08-31 | Cell network management of ventilating |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206109421U true CN206109421U (en) | 2017-04-19 |
Family
ID=58516132
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621021167.7U Active CN206109421U (en) | 2016-08-31 | 2016-08-31 | Cell network management of ventilating |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206109421U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106119111A (en) * | 2016-08-31 | 2016-11-16 | 上海戈洛思生物科技有限公司 | A kind of cell ventilating net tube |
-
2016
- 2016-08-31 CN CN201621021167.7U patent/CN206109421U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106119111A (en) * | 2016-08-31 | 2016-11-16 | 上海戈洛思生物科技有限公司 | A kind of cell ventilating net tube |
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