Ventilating device for fermentation process and bioreactor
Technical Field
The utility model belongs to the technical field of biological fermentation, especially, relate to an aerating device and bioreactor that is arranged in fermentation process to gas demand height.
Background
In the prior art, the ventilation device in the fermentation process only leads the external sterile gas into the liquid in the tank body of the bioreactor directly through a straight-through pipeline, so that the gas easily forms larger bubbles in the liquid, the retention time is short, the utilization rate is low, the utilization rate is slow, the efficiency is low, and the gas can be maintained only by continuously increasing the gas flow rate along with the progress of the culture process so as to achieve the target culture parameter value. However, there are some problems with this, such as: great gas flow can lead to the fact the damage to the culture in the bioreactor to the bubble that produces is great more, and to some culture solution that produce the bubble easily, the bubble is more can bring unfavorable to the observation of cultivateing the in-process in the bioreactor jar body moreover, leads to the fact the influence to the cultivation, and in addition, more bubble still gives vent to anger the risk that the filter core blockked up with the jar body, leads to whole cultivation process's interruption failure easily.
In addition, in the prior art, the gas entering the ventilating device is not filtered, so that the gas entering the ventilating device easily contains impurities to influence the culture process.
SUMMERY OF THE UTILITY MODEL
In order to overcome current air-breather's gas utilization rate low and produce more bubble easily and to the influence etc. of cultivateing the process, the utility model provides an air-breather and bioreactor for among the fermentation process, the device can not only reduce the gas quantity and improve gas utilization rate, can effectively reduce the bubble that the fermentation cultivateed the in-process and produced moreover, filters the impurity among the gas.
Realize above-mentioned purpose the utility model discloses a following technical scheme does: a ventilation device used in a fermentation process comprises a straight-through air pipe and a circular ring pipe positioned below the straight-through air pipe, wherein one end of the straight-through air pipe is an air inlet, the other end of the straight-through air pipe is connected with the circular ring pipe to form a connecting part for connecting the straight-through air pipe and the circular ring pipe in a seamless mode, and the plane of the circular ring pipe is perpendicular to the straight-through air pipe; the circular ring pipe is provided with a plurality of air outlet holes which are uniformly distributed on the circumferential curved surface at the bottom of the circular ring pipe, and the air filled from the air inlet enters the circular ring pipe from the connecting part after passing through the straight air pipe and then is uniformly dispersed in the culture solution through the air outlet holes.
A more preferred scheme is as follows: the straight-through air pipe comprises a clamping position, the clamping position is arranged above the air inlet, and the clamping position is used for fixing the straight-through air pipe.
Furthermore, the straight-through air pipe comprises an air guide port, the air guide port is arranged in the connecting part, and air entering the straight-through air pipe enters the circular ring pipe through the air guide port.
Furthermore, the connecting part of the straight air pipe and the circular ring pipe in seamless connection adopts a welding mode.
Further, the straight air pipe and the circular ring pipe are made of stainless steel materials.
Furthermore, the bioreactor comprises a tank body, a tank opening, a fastening piece and an air inlet pipe, wherein the tank body is used for placing the ventilating device and the culture solution, the tank opening is formed in the upper end of the tank body, the fastening piece is arranged between the tank opening and the air inlet pipe, the fastening piece is used for fastening the clamping position of the ventilating device, and the fastening piece plays a role in fixing the ventilating device.
Furthermore, the tank body can also be provided with a culture solution for supplying microorganisms, plants or animals (or tissues) to grow and reproduce.
Furthermore, the air inlet pipe is provided with an air filtering piece for filtering the air impurities in the air inlet pipe.
Furthermore, the bioreactor is made of stainless steel materials and has the characteristics of firm materials, high chemical acid and alkali resistance, impact resistance, corrosion resistance and high temperature resistance.
Furthermore, one end of the circular ring pipe is provided with a circular shape, and the circular ring pipe has the characteristics of attractive appearance, smooth inner wall and small resistance.
The beneficial effects of the utility model reside in that:
(1) the utility model is provided with a straight-through air pipe and a circular ring pipe, the ventilation device comprises a straight-through air pipe and a circular ring pipe positioned below the straight-through air pipe, one end of the straight-through air pipe is an air inlet, the other end of the straight-through air pipe is connected with the circular ring pipe and forms a connecting part for seamlessly connecting the straight-through air pipe and the circular ring pipe, and the connecting part adopts a welding mode to prevent air leakage in the process that air enters the circular ring pipe from the straight-through air pipe;
(2) the plane of the circular ring pipe of the utility model is vertical to the straight-through air pipe, so that the ventilating device is convenient to be placed in the bioreactor, and the ventilating device is vertical to the plane, so that no other bending or turning occurs, and gas can be directly introduced into the circular ring pipe from the gas guide port, thereby the reaction of the culture solution is faster and the reaction efficiency is improved;
(3) the circular ring pipe is provided with the plurality of air outlet holes which are uniformly distributed on the circumferential curved surface at the bottom of the circular ring pipe, and the air holes are formed at the bottom of the circular ring pipe, so that the air can come out from the bottom end of the circular ring pipe, and the permeation between substances in the culture solution is promoted;
(4) the utility model discloses a plurality of ventholes homodisperse lets gas and culture solution intensive mixing in the culture solution, has improved the mixing efficiency of gas and culture solution, and the bubble that produces in the fermentation culture process of can effectual reduction simultaneously.
(5) Be equipped with gas filtration piece on the intake pipe among the bioreactor for filter the gaseous impurity of intake pipe, avoid gaseous impurity to cause the influence to the cultivation process.
Drawings
FIG. 1 is a schematic structural diagram of an aeration device and a bioreactor used in a fermentation process according to the present invention;
FIG. 2 is a schematic structural diagram of an aeration device used in a fermentation process according to the present invention;
FIG. 3 is a schematic view of another angle structure of the aeration device used in the fermentation process of the present invention;
fig. 4 is an enlarged schematic view of the structure of the straight-through air pipe of the present invention.
In the figure, the aeration device 1, the straight-through air pipe 11, the air inlet 111, the air guide port 112, the clamping position 113, the joining part 114, the circular ring pipe 12, a plurality of air outlet holes 121, the bioreactor 2, the tank opening 21, the tank body 22, the fastening part 23, the air inlet pipe 24 and the filtering piece 241.
Detailed Description
Referring to fig. 1-4, an aeration device 1 and a bioreactor 2 for fermentation process, including an aeration device 1 and a bioreactor 2, where the aeration device 1 includes a straight-through air pipe 11 and a circular loop pipe 12 located below the straight-through air pipe 11, one end of the straight-through air pipe 11 is an air inlet 111, and the other end is connected with the circular loop pipe 12 and forms a connection part 114 that connects the straight-through air pipe 11 and the circular loop pipe 12 without seams, the plane of the circular loop pipe 12 is perpendicular to the straight-through air pipe 11, the perpendicular arrangement is convenient for placing the aeration device 1 in the bioreactor 2, and the vertical design is vertical, there is no other bend or bend, the air can be directly introduced into the circular loop pipe 12 from an air guide port 112, the reaction of culture solution can be carried out more quickly, and the reaction efficiency is improved;
the ring body of the circular ring pipe 12 is hollow, the circular ring pipe 12 is provided with a plurality of air outlets 121, the air outlets 121 are uniformly distributed on the circumferential curved surface at the bottom of the circular ring pipe 12, and the reason that the air outlets are arranged at the bottom of the circular ring pipe 12 is as follows: because the gas is filled from the gas inlet 111 and enters the through gas pipe 11, then enters the interior of the ring body of the circular ring pipe 12 from the gas guide port 112, and then comes out from the plurality of gas outlet holes 121, because the density of the gas is smaller than that of the culture solution in the bioreactor 2, the gas rises, and the gas can promote the culture solution in the bioreactor 2 to perform substance permeation and reaction in the rising process, so that the permeation and reaction efficiency is improved, and as the time passes little by little, the bubbles can rise to the upper surface of the culture solution and can generate little bubbles, and because the gas outlet holes are arranged at the bottom of the circular ring pipe 12, the rising time of the gas is prolonged, and the quantity of the bubbles is favorably reduced.
The number of the air outlet holes 121 is 30, and the diameter of each air hole is 1 mm.
The air outlets 121 are located on the same plane, the intervals between the air holes are consistent, and the air outlets 121 are arranged along the circumferential curved surface at the bottom of the circular ring pipe 12 to form a centrosymmetric pattern, which is more attractive.
The straight air pipe 11 comprises a clamping position 113, the clamping position 113 is arranged above the air inlet 111, and the clamping position 113 is used for fixing the straight air pipe 11.
The air inlet 111 is fixedly connected with the clamping position 113.
The through air pipe 11 comprises an air guide port 112, the air guide port 112 is arranged in a connecting part 114, and air entering the through air pipe 11 enters the circular ring pipe 12 through the air guide port 112.
The connecting part 114 of the straight-through air pipe 11 and the circular ring pipe 12 which are connected in a seamless mode is welded.
The bioreactor 2 comprises a tank body 22, a tank opening 21, a fastening piece 23 and an air inlet pipe 24, wherein the tank body 22 is used for placing the air breather 1 and culture solution, the tank opening 21 is arranged at the upper end of the tank body 22, the fastening piece 23 is arranged between the tank opening 21 and the air inlet pipe 24, the fastening piece 23 is used for fastening the clamping position 113 of the air breather 1, and the fastening piece 23 plays a role in fixing the air breather 1.
The tank 22 may also contain a culture solution for the growth and reproduction of microorganisms, plants or animals (or tissues).
The air inlet pipe 24 is provided with an air filter 241 for filtering the air impurities in the air inlet pipe 24.
The straight gas pipe 11, the circular ring pipe 12 and the bioreactor 2 are made of stainless steel materials, and have the characteristics of firm materials, high chemical acid and alkali resistance, impact resistance, corrosion resistance and high temperature resistance.
The first embodiment is as follows: the utility model has the advantages that:
the utility model discloses an effect that ventilator 1 evenly scatters by a plurality of ventholes 121 except leading-in circular ring canal 12 back with the gas of direct trachea 11, ventilator 1 can also play the effect of stirring, can be arranged in the culture solution of bioreactor 2 to stir.
Example two: the utility model discloses an instruction for use:
firstly, culture solution required by fermentation is placed in a bioreactor 2, and a ventilation device 1 is buckled on the bioreactor 2 through a buckling piece 23;
secondly, introducing external gas required by the fermentation culture solution from the gas inlet pipe 24, filtering the gas by a gas filter 241 and then entering the straight-through gas pipe 11;
thirdly, the gas entering the straight-through gas pipe 11 enters the circular ring pipe 12 under the action of gas pressure;
then, the gas entering the circular ring pipe 12 is uniformly discharged through a plurality of gas outlet holes 121 under the action of the gas pressure;
finally, the gas is scattered through a plurality of small holes and discharged into the culture solution of the bioreactor 2, the gas dispersed in the bioreactor 2 forms a plurality of bubbles with small volume, the mixing efficiency of the gas and the culture solution is increased, and after the gas is dispersed and stays in the bioreactor 2 for a period of time, the bubbles formed when the gas is discharged out of the liquid surface are small and the number is small.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the scope of the invention, i.e. the present invention is intended to cover all equivalent variations and modifications within the scope of the present invention.