CN202543192U - Cell reactor and lifting type stirrer thereof - Google Patents
Cell reactor and lifting type stirrer thereof Download PDFInfo
- Publication number
- CN202543192U CN202543192U CN 201220118127 CN201220118127U CN202543192U CN 202543192 U CN202543192 U CN 202543192U CN 201220118127 CN201220118127 CN 201220118127 CN 201220118127 U CN201220118127 U CN 201220118127U CN 202543192 U CN202543192 U CN 202543192U
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- CN
- China
- Prior art keywords
- delivery pipe
- riser tube
- whisking appliance
- hoisting type
- type whisking
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- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a lifting type stirrer of a cell reactor. The stirrer comprises a stirring shaft (1) and a lifting pipe (2), wherein the lifting pipe (2) is connected below the stirring shaft (1); the periphery of the lifting pipe (2) is provided with at least two discharge pipe layers (3); the discharge pipe layers (3) are axially arranged along the lifting pipe (2); and at least one discharge pipe (31) is arranged on each discharge pipe layer (3). By adoption of the structure, the requirement of stirring cultivation solutions with different volumes can be met, so that the cultivation solutions with different volumes can relatively uniformly flow circularly and are fully mixed; and therefore, a cell cultivation method that the cultivation solutions are added in batches is implemented, and the cultivation solutions in a cultivation tank are stirred uniformly. The utility model also provides the cell reactor which comprises the lifting stirrer and has the same advantages.
Description
Technical field
The utility model relates to biological technical field, relates in particular to a kind of hoisting type whisking appliance that is used for cell reactor.In addition, the utility model also relates to a kind of cell reactor that comprises above-mentioned hoisting type whisking appliance.
Background technology
Mammiferous cell cultures refers under the cells in vitro condition, keeps cell growth and the technology of breeding with nutrient solution.Cell reactor generally includes culture tank, ventpipe and hoisting type whisking appliance.Hold nutrient solution and cell in the culture tank, for cell provides growing environment, ventpipe is used for to the culture tank delivering gas, and cell is drawn the oxygen branch in nutrient solution and the gas in culturing process, for the needs of self growing.The hoisting type whisking appliance is used for the nutrient solution of culture tank is stirred, and is evenly distributed with the nutrient solution that guarantees cell and interpolation.
In the prior art, the hoisting type whisking appliance generally includes stir shaft, is connected in the riser tube of stir shaft below, and the delivery pipe of being located at the individual layer setting of riser tube periphery.After being added into nutrient solution in the culture tank; Driving the hoisting type whisking appliance rotates its stir shaft; The nutrient solution of riser tube bottom is absorbed into riser tube inside and moves upward, flows out through the delivery pipe of being located at the riser tube periphery then, thus the even stirring of the nutrient solution in the realization culture tank.
Along with the development of cell culture technology, gradually adopt the training method of a kind of " batch formula stream add " in the cell cultivation process, promptly be divided into the nutrient solution of in culture tank, annotating gradually of several time periods.For example, during beginning, only in culture tank, add less nutrient solution (for example 1/4 to 1/3 volume); After for some time (for example 1 day to 2 days), add certain solution amount again ... so carry out, up to total work volume of filling it up with culture tank.
Thus, if the hoisting type whisking appliance that still adopts above-mentioned individual layer to be provided with can't stir by the nutrient solution that capacity is less in whipping process, therefore the hoisting type whisking appliance of above-mentioned individual layer setting can't satisfy the production technique of " batch formula stream adds ".
In view of this, demand urgently the structure of existing hoisting type whisking appliance being carried out further optimization design, make it and can satisfy the training method of " batch formula stream adds ", the nutrient solution in the culture tank is stirred to above-mentioned technical problem.
The utility model content
The technical problem that the utility model will solve is that a kind of hoisting type whisking appliance that is used for cell reactor is provided, and this hoisting type whisking appliance can satisfy the cell culture processes of " batch formula stream adds ", and nutrient solution in the culture tank is stirred.Another technical problem that the utility model will solve is that a kind of cell reactor that comprises above-mentioned hoisting type whisking appliance is provided.
For solving the problems of the technologies described above, the utility model provides a kind of hoisting type whisking appliance that is used for cell reactor, comprises stir shaft and riser tube, and said riser tube is connected in said stir shaft below; The periphery of said riser tube is provided with at least two delivery pipe layers, the axial setting of at least two said riser tubes in said delivery pipe layer edge, and each delivery pipe layer is provided with at least one delivery pipe.
Preferably, the number of said delivery pipe layer is three, three said delivery pipe layers axially evenly being provided with along said riser tube.
Preferably, each said delivery pipe layer comprises three delivery pipes, and three said delivery pipes are symmetrically set in the periphery of said riser tube.
Preferably, the axis normal of each said delivery pipe is in the axis of riser tube.
Preferably, the axis of the said relatively delivery pipe in the outer face of each said delivery pipe is obliquely installed, and the sense of rotation of the said riser tube in edge, and the length of said delivery pipe is shortened by long.
Preferably, the angle that axis was of the outer face of said delivery pipe and said delivery pipe is 60 degree.
The utility model provides a kind of hoisting type whisking appliance that is used for cell reactor, and the periphery of its riser tube is provided with at least two delivery pipe layers, and at least two delivery pipe layers are along the axial setting of riser tube, and each delivery pipe layer is provided with at least one delivery pipe.In the concrete scheme, the number of above-mentioned delivery pipe layer can be three, three said delivery pipe layers axially evenly being provided with along said riser tube.Hoisting type whisking appliance with this structure is an example below, specifically introduces the working process of above-mentioned hoisting type whisking appliance.
Adopt this structure; In the training method of " batch formula stream adds ", when the liquid level that adds nutrient solution is positioned at place, I planimetric position, starts the hoisting type whisking appliance and make stir shaft rotate; At this moment the delivery pipe layer of riser tube bottom works; It is inner and move upward to make that nutrient solution gets into riser tube in rotary course, flows into each delivery pipe of the delivery pipe layer of being located at the riser tube bottom then, flows out from the outer end of each delivery pipe again.When the liquid level that adds for the second time nutrient solution is positioned at the II planimetric position; Starting the hoisting type whisking appliance makes stir shaft rotate; At this moment two delivery pipe layers at riser tube bottom and middle part work and make nutrient solution in rotary course, get into riser tube inside and move upward; Flow into each delivery pipe of two delivery pipe layers being located at riser tube bottom and middle part then respectively, flow out from the outer end of each delivery pipe again.When the liquid level that adds nutrient solution for the third time is positioned at the III planimetric position; Starting the hoisting type whisking appliance makes stir shaft rotate; At this moment three delivery pipe layers at riser tube bottom, middle part and top work simultaneously; It is inner and move upward to make that nutrient solution gets into riser tube in rotary course, flows into each delivery pipe of three delivery pipe layers being located at riser tube bottom, middle part and top then respectively, flows out from the outer end of each delivery pipe again.
This shows; The hoisting type whisking appliance of employing said structure can satisfy the stirring requirement of differing capacities; Make the nutrient solution of differing capacities all can circulate comparatively equably, thorough mixing, therefore; Above-mentioned hoisting type whisking appliance has satisfied the cell culture processes of " batch formula stream adds ", and nutrient solution in the culture tank is stirred.
The utility model also provides a kind of cell reactor, comprises culture tank; Also comprise aforesaid hoisting type whisking appliance, said hoisting type whisking appliance is installed in the said culture tank.
Because above-mentioned hoisting type whisking appliance has above-mentioned technique effect, therefore, comprises that the cell reactor of this hoisting type whisking appliance also should have identical technique effect, repeats no more at this.
Description of drawings
Fig. 1 provides the structural representation of a kind of embodiment of hoisting type whisking appliance for the utility model;
Fig. 2 is the vertical view of the delivery pipe layer among Fig. 1.
Wherein, Reference numeral among Fig. 1 to Fig. 2 and the corresponding relation between the component names are:
Stir shaft 1; Riser tube 2; Delivery pipe layer 3; Delivery pipe 31; Outer face 311.
Embodiment
The core of the utility model is that a kind of hoisting type whisking appliance that is used for cell reactor is provided, and this hoisting type whisking appliance can satisfy the cell culture processes of " batch formula stream adds ", and nutrient solution in the culture tank is stirred.Another core of the utility model is that a kind of cell reactor that comprises above-mentioned hoisting type whisking appliance is provided.
In order to make those skilled in the art understand the technical scheme of the utility model better, the utility model is done further to specify below in conjunction with accompanying drawing and specific embodiment.
Please refer to Fig. 1 and Fig. 2, Fig. 1 provides the structural representation of a kind of embodiment of hoisting type whisking appliance for the utility model; Fig. 2 is the vertical view of the delivery pipe layer among Fig. 1.
In a kind of embodiment, the hoisting type whisking appliance that is used for cell reactor that the utility model provided comprises stir shaft 1, riser tube 2 and at least two delivery pipe layers 3.Stir shaft 1 is the rotation axis of hoisting type whisking appliance, and it is connected with the PWR PLT of hoisting type whisking appliance usually; Riser tube 2 is generally hollow pipe, and it is connected in the below of stir shaft 1; At least two delivery pipe layers 3 are located at the periphery of riser tube 2, and along the axial setting of riser tube 2, each delivery pipe layer 3 is provided with at least one delivery pipe 31.
In the concrete scheme, as shown in Figure 1, the number of above-mentioned delivery pipe layer 3 can be three, three said delivery pipe layers 3 axially evenly being provided with along said riser tube 2.Hoisting type whisking appliance with this structure is an example below, specifically introduces the working process of above-mentioned hoisting type whisking appliance.
Adopt this structure, as shown in Figure 1, in the training method of " batch formula stream adds "; When the liquid level that adds nutrient solution is positioned at place, I planimetric position; Start the hoisting type whisking appliance and make stir shaft 1 rotate, the delivery pipe layer 3 that be located at riser tube 2 bottoms this moment works, and makes nutrient solution in rotary course, get into riser tube 2 inside and moves upward; Flow into each delivery pipe 31 of the delivery pipe layer 3 of being located at riser tube 2 bottoms then, flow out from the outer end of each delivery pipe 31 again.
When the liquid level that adds for the second time nutrient solution is positioned at the II planimetric position; Starting the hoisting type whisking appliance makes stir shaft 1 rotate; Two delivery pipe layers 3 that be located at riser tube 2 bottoms and middle part this moment work; It is inner and move upward to make that nutrient solution gets into riser tube 2 in rotary course, flows into each delivery pipe 31 of two delivery pipe layers 3 being located at riser tube 2 bottoms and middle part then respectively, again from the outer end outflow of each delivery pipe 31.
When the liquid level that adds nutrient solution for the third time is positioned at the III planimetric position; Starting the hoisting type whisking appliance makes stir shaft 1 rotate; Three delivery pipe layers 3 that be located at riser tube 2 bottoms, middle part and top this moment all work; It is inner and move upward to make that nutrient solution gets into riser tube 2 in rotary course, flows into each delivery pipe 31 of three delivery pipe layers 3 being located at riser tube 2 bottoms, middle part and top then respectively, flows out from the outer end of each delivery pipe 31 again.
This shows; The hoisting type whisking appliance of employing said structure can satisfy the stirring requirement of differing capacities; Make the nutrient solution of differing capacities all can circulate comparatively equably, thorough mixing, therefore; Above-mentioned hoisting type whisking appliance has satisfied the cell culture processes of " batch formula stream adds ", and nutrient solution in the culture tank is stirred.
Need to prove; Above-mentioned hoisting type whisking appliance is not limited in the structure that comprises three delivery pipe layers 3; Can also it be set to comprise the hoisting type whisking appliance of four delivery pipe layers 3 or two delivery pipe layers 3, the user can select according to the different batches of " batch formula stream adds " voluntarily.Certainly, above-mentioned embodiment does not specifically limit the number of the delivery pipe 31 that each delivery pipe layer 3 comprises, the hoisting type whisking appliance of every at least two delivery pipe layers 3 of axial setting at riser tube 2 all should be worked as in the protection domain that belongs to the utility model.
In addition, the noun of locality that occurs among this paper " outward " refers among Fig. 2 the xsect at riser tube 2, points to its perisporium direction by the center of circle of riser tube 2; " interior " refers among Fig. 2 the xsect at riser tube 2, pointed to the direction in its center of circle by the perisporium of riser tube 2; Should be appreciated that these nouns of locality are that benchmark is set up with the accompanying drawing, their appearance should not influence the protection domain of the utility model.
In another kind of embodiment, above-mentioned each delivery pipe layer 3 comprises that 31, three said delivery pipes 31 of three delivery pipes are symmetrically set in the periphery of said riser tube 2.Like this, in whipping process, be positioned at three delivery pipe 31 stress balances of same delivery pipe layer 3, the resistance of generation is less, and can produce bigger horizontal whipping force, can guarantee nutrient solution is stirred.In addition, adopt each delivery pipe layer 3 to comprise the structure of three delivery pipes 31, make that also the production cost of hoisting type whisking appliance is lower.Certainly, above-mentioned each delivery pipe layer 3 also can be provided with the delivery pipe 31 of other numbers, for example can also comprise four delivery pipes, six delivery pipes etc.
Further in the scheme, the axis of above-mentioned each delivery pipe 31 can be perpendicular to the axis of riser tube 2.Adopt this structure, when delivery pipe 31 under the drive of stir shaft 1 when the axis of riser tube 2 rotates, delivery pipe 31 is less in impact and shearing force that vertical direction receives, has reduced the stirring resistance, more helps nutrient solution is stirred.
In the scheme, the axis of the outer face 311 relative delivery pipes 31 of above-mentioned each delivery pipe 31 is obliquely installed more specifically, and along the sense of rotation of riser tube 2, the length of delivery pipe 31 is shortened by long.Adopt this structure, as shown in Figure 2, in whipping process, make the shearing force that in the process that the axis around riser tube 2 rotates, receives at delivery pipe 31 further reduce, promptly reduced the impact of delivery pipe 31 in horizontal plane, more help nutrient solution is stirred.
Preferably; The outer face 311 of above-mentioned delivery pipe 31 can be 60 degree with the angle that axis was of said delivery pipe 31; This angle is the structure that is suitable for above-mentioned three symmetrically arranged delivery pipes 31; Make the stress balance of three symmetrically arranged delivery pipes 31, further increase the job stability of hoisting type whisking appliance.Certainly, the angle between the axis of the outer face 311 of above-mentioned delivery pipe 31 and delivery pipe 31 also can adopt other angles.
In addition, the utility model also provides a kind of cell reactor, comprises culture tank, also comprises aforesaid hoisting type whisking appliance, and said hoisting type whisking appliance is installed in the said culture tank.
Because above-mentioned hoisting type whisking appliance has above-mentioned technique effect, therefore, comprises that the cell reactor of this hoisting type whisking appliance also should have identical technique effect, repeats no more at this.
More than a kind of cell reactor and hoisting type whisking appliance thereof that the utility model provided have been carried out detailed introduction.Used concrete example among this paper the principle and the embodiment of the utility model are set forth, the explanation of above embodiment just is used to help to understand the method and the core concept thereof of the utility model.Should be understood that; For those skilled in the art; Under the prerequisite that does not break away from the utility model principle, can also carry out some improvement and modification to the utility model, these improvement and modification also fall in the protection domain of the utility model claim.
Claims (7)
1. a hoisting type whisking appliance that is used for cell reactor comprises stir shaft (1) and riser tube (2), and said riser tube (2) is connected in said stir shaft (1) below; It is characterized in that the periphery of said riser tube (2) is provided with at least two delivery pipe layers (3), at least two said delivery pipe layers (3) are along the axial setting of said riser tube (2), and each delivery pipe layer (3) is provided with at least one delivery pipe (31).
2. the hoisting type whisking appliance that is used for cell reactor according to claim 1 is characterized in that the number of said delivery pipe layer (3) is three, and three said delivery pipe layers (3) axially evenly are provided with along said riser tube (2).
3. the hoisting type whisking appliance that is used for cell reactor according to claim 2 is characterized in that, each said delivery pipe layer (3) comprises three delivery pipes (31), and three said delivery pipes (31) are symmetrically distributed in the periphery of said riser tube (2).
4. the hoisting type whisking appliance that is used for cell reactor according to claim 3 is characterized in that the axis normal of each said delivery pipe (31) is in the axis of said riser tube (2).
5. the hoisting type whisking appliance that is used for cell reactor according to claim 4; It is characterized in that; The axis of the said relatively delivery pipe in outer face (311) (31) of each said delivery pipe (31) is obliquely installed; And along the sense of rotation of said riser tube (2), the length of said delivery pipe (31) is shortened by long.
6. the hoisting type whisking appliance that is used for cell reactor according to claim 5 is characterized in that, the outer face (311) of said delivery pipe (31) is 60 degree with the angle that axis was of said delivery pipe (31).
7. a cell reactor comprises culture tank; It is characterized in that also comprise each described hoisting type whisking appliance like claim 1-6, said hoisting type whisking appliance is installed in the said culture tank.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220118127 CN202543192U (en) | 2012-03-26 | 2012-03-26 | Cell reactor and lifting type stirrer thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220118127 CN202543192U (en) | 2012-03-26 | 2012-03-26 | Cell reactor and lifting type stirrer thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202543192U true CN202543192U (en) | 2012-11-21 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201220118127 Expired - Lifetime CN202543192U (en) | 2012-03-26 | 2012-03-26 | Cell reactor and lifting type stirrer thereof |
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| Country | Link |
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| CN (1) | CN202543192U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255733A (en) * | 2015-11-27 | 2016-01-20 | 上海日泰医药设备工程有限公司 | Stirring device for cell tank |
| CN105331538A (en) * | 2015-11-27 | 2016-02-17 | 上海日泰医药设备工程有限公司 | Cell tank |
| CN105331519A (en) * | 2015-11-27 | 2016-02-17 | 上海日泰医药设备工程有限公司 | Stirring device for fermentation tank |
| CN106834099A (en) * | 2017-03-29 | 2017-06-13 | 江苏丰泽生物工程设备制造有限公司 | Bioreactor low sheraing hoisting type aeration mixing device |
-
2012
- 2012-03-26 CN CN 201220118127 patent/CN202543192U/en not_active Expired - Lifetime
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105255733A (en) * | 2015-11-27 | 2016-01-20 | 上海日泰医药设备工程有限公司 | Stirring device for cell tank |
| CN105331538A (en) * | 2015-11-27 | 2016-02-17 | 上海日泰医药设备工程有限公司 | Cell tank |
| CN105331519A (en) * | 2015-11-27 | 2016-02-17 | 上海日泰医药设备工程有限公司 | Stirring device for fermentation tank |
| CN105331538B (en) * | 2015-11-27 | 2017-09-29 | 上海日泰医药设备工程有限公司 | A kind of cell tank |
| CN105255733B (en) * | 2015-11-27 | 2018-06-19 | 上海日泰医药设备工程有限公司 | A kind of cell tank agitating device |
| CN106834099A (en) * | 2017-03-29 | 2017-06-13 | 江苏丰泽生物工程设备制造有限公司 | Bioreactor low sheraing hoisting type aeration mixing device |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20121121 |
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| CX01 | Expiry of patent term |