CN108410734A - A kind of biological reactor for cell culture - Google Patents
A kind of biological reactor for cell culture Download PDFInfo
- Publication number
- CN108410734A CN108410734A CN201810475273.XA CN201810475273A CN108410734A CN 108410734 A CN108410734 A CN 108410734A CN 201810475273 A CN201810475273 A CN 201810475273A CN 108410734 A CN108410734 A CN 108410734A
- Authority
- CN
- China
- Prior art keywords
- ring
- cell culture
- agitating shaft
- tank body
- biological reactor
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The present invention provides a kind of biological reactor for cell culture, belong to technical field of cell culture, including tank body, the top of tank body is equipped with enterprising gas port, and lower section is equipped with lower air inlet, and tank base center is equipped with magnetic agitation mechanism, magnetic agitation mechanism is connected with the agitating shaft positioned at tank interior, the top of agitating shaft is equipped with upper blade, and agitating shaft is equipped with the first rabbling mechanism and the second rabbling mechanism to distribute alternately successively from top to bottom, and the length of the second rabbling mechanism is longer than the length of the first rabbling mechanism；Microvesicle ring is equipped with below agitating shaft, microvesicle ring includes the first air intake lever that hollow point of ring of the gradually smaller at least two of diameter and connection divide ring from top to bottom, it is connected with the second air intake lever between first air intake lever and lower air inlet, the lower surface of ring is divided to be equipped with several equally distributed stomatas.Present invention ventilation is uniform and efficient, and cell and nutriment are uniformly mixed, and disclosure satisfy that big volume tank body production, yield is high and product quality is uniform.
Description
Technical field
The invention belongs to technical field of cell culture, and in particular to a kind of biological reactor for cell culture.
Background technology
Bioreactor is the core equipment of mass cell suspension culture, can effectively increase cell-unit volume
Culture density is the mainstay of such product large-scale production.China be world's vaccine product largest production state and
Maximum uses state, and with annual 15% speed increase；In recent years, the development of antibody drug and the market demand also increase substantially.
To improve production efficiency and product quality, production cost is effectively reduced, is changed with the backwardness production technology of spinner culture cell, it is anxious
Need the animal cell culture reactor of the above production scale of kilolitre grade.
Existing cell culture reactor volume is generally less than normal, predominantly stays in 3000L at present hereinafter, low output, energy consumption
Height, labour demand is big, product quality is inhomogenous, pollution is difficult to control, therefore comprehensive performance and production efficiency is not high.
It is anti-that existing application for a patent for invention cell reactor (application No. is CN201710482335.5) discloses a kind of cell
Device is answered, its key points of the technical solution are that including tank body, the blender being set in tank body, the driving machine for driving the blender
Structure, the snorkel being provided on the tank body inside and outside connection tank body, the outer end of the snorkel is connected to air source, the snorkel
One end in tank body is connected with closed fibre pipe, and the fibre pipe is connected to the snorkel.The invention solves existing
Some cell reactors cause cellular damage due to ventilation leads to the problem of bubble, to achieve the effect that improve cell density,
But ventilation lack of homogeneity and efficiency it is low, cannot be satisfied the bioreactor of big volume.
Therefore it is uniform and efficient to be badly in need of a kind of ventilation, cell and nutriment are uniformly mixed, and disclosure satisfy that big volume tank
Body produces, the biological reactor for cell culture that yield is high and product quality is uniform.
Invention content
The object of the present invention is to provide a kind of biological reactor for cell culture, ventilate uniform and efficient, cell and nutrition
Material mixing is uniform, disclosure satisfy that big volume tank body production, yield is high and product quality is uniform.
The present invention provides the following technical solutions：
The top of a kind of biological reactor for cell culture, including tank body, the tank body is equipped with enterprising gas port, and lower section is equipped with down
Air inlet, the tank base center are equipped with magnetic agitation mechanism, and the magnetic agitation mechanism is connected in the tank body
The agitating shaft in portion, the top of the agitating shaft are equipped with upper blade, and the agitating shaft is equipped with the to distribute alternately successively from top to bottom
One rabbling mechanism and the second rabbling mechanism；
First rabbling mechanism includes the fixed ring for being fixed on the agitating shaft, and the fixed ring is connected with several first
Horizontal axis, the first horizontal the tip of the axis are equipped with the first blade；Second rabbling mechanism includes affixed with the agitating shaft
Two horizontal axis, the second horizontal the tip of the axis are equipped with transverse slat, and the transverse slat is connected with the second blade, and the length of second horizontal axis is long
In the length of first horizontal axis；
Microvesicle ring is equipped with below the agitating shaft, the microvesicle ring includes gradually smaller at least two of diameter from top to bottom
The first air intake lever for dividing ring described in hollow point of ring and connection, with the second air inlet between first air intake lever and the lower air inlet
Bar is connected, and the lower surface for dividing ring is equipped with several equally distributed stomatas.
Preferably, described that ring is divided to be with circular rings jaggy, two ends for being divided to ring are equipped with removably
Plug, convenient for dividing the cleaning and maintenance of ring.
Preferably, the plug divides the end of ring to be to be threadedly coupled with described, convenient disassembly.
Preferably, it is connected with silk mouth nut between first air intake lever and second air intake lever, convenient disassembly；Described
One air intake lever is in 30 degree of angles with interplanar, and effect is that microvesicle ring is placed in below rabbling mechanism, and does not have with rabbling mechanism
Scraping collision.
Preferably, the distance of a diameter of 0.1 millimeter of the stomata, the two neighboring stomata is 1 centimetre.
Preferably, the height of the enterprising gas port ensures surface layer air inlet not higher than 50 centimetres of liquid level in the tank body
Directly in piping and druming to cell culture liquid, more foam is prevented.
Preferably, the magnetic agitation mechanism includes the motor being sequentially connected, outer transmission shaft and intermediate solid drive shaft, the unofficial biography
Opposite outer magnetic assembly and interior magnetic assembly, the bottom of the intermediate solid drive shaft and the agitating shaft are equipped between moving axis and the intermediate solid drive shaft
End is connected, and power transmits the electromagnetic force for fully relying on magnet steel generation, and mixing effect is good, and is not easy microbiological contamination.
Preferably, the magnetic agitation mechanism and the joint of the tank body are equipped with separation sleeve, advantageously ensure that in tank body
Gnotobasis.
Preferably, the inner wall of the tank body is equipped with baffle, is matched with rabbling mechanism, realizes the height of low-shearing force off field
Imitate the uniform mixing of oxygen transfer, cell and nutriment.
The beneficial effects of the invention are as follows：
(1) the first rabbling mechanism and the second rabbling mechanism that agitating shaft of the invention is equipped with upper blade and distributes alternately,
Upper blade can be stirred the liquid above tank body, and the first rabbling mechanism can be stirred the liquid at tank body center, and second
Rabbling mechanism can be stirred the liquid near inner tank wall, to realize in tank body the uniform of cell everywhere and nutriment
Mixing.
(2) present invention is equipped with microvesicle ring, and microvesicle ring includes the gradually smaller several hollow point of rings of diameter from top to bottom, can
It ventilates from different height and all directions to the cell of tank body, stomata is set to the lower surface for dividing ring, and cell can be prevented broken
Air-hole blockage can be met the cell culture of 7500L large volume tank bodies by piece, to greatly improve yield, also be improved
Product quality.
(3) present invention hits exactly magnetic agitation using bottom, and sealing effect is good, reduces microbiological contamination risk, is conducive to cell
Smoothly culture.
Description of the drawings
Attached drawing is used to provide further understanding of the present invention, and a part for constitution instruction, the reality with the present invention
It applies example to be used to explain the present invention together, not be construed as limiting the invention.In the accompanying drawings：
Fig. 1 is the structural schematic diagram of the present invention；
Fig. 2 is the structural schematic diagram of the first rabbling mechanism；
Fig. 3 is the main structure diagram of microvesicle ring in embodiment；
Fig. 4 is the present invention looks up structural representation of microvesicle ring in embodiment；
Fig. 5 is the structural schematic diagram of magnetic agitation mechanism.
In figure label for：1, tank body；2, enterprising gas port；3, agitating shaft；4, upper blade；5, the first rabbling mechanism；51, fixed
Ring；52, the first horizontal axis；53, the first blade；6, the second horizontal axis；7, transverse slat；8, the second blade；9, baffle；10, lower air inlet；
11, the second air intake lever；12, the first air intake lever；13, upper ring；14, lower ring；15, magnetic agitation mechanism；151, motor；152, unofficial biography
Moving axis；153, interior magnetic assembly；154, outer magnetic assembly；155, separation sleeve；156, intermediate solid drive shaft；16, silk mouth nut；17, stomata；
18, plug.
Specific implementation mode
As shown in Figure 1, a kind of biological reactor for cell culture, including tank body 1, the top of tank body 1 are equipped with enterprising gas port 2,
Lower section is equipped with lower air inlet 10, and the height of enterprising gas port 2 ensures that surface layer air inlet is not straight higher than 50 centimetres of liquid level in tank body 1
It connects in piping and druming to cell culture liquid, prevents more foam.
As depicted in figs. 1 and 2,1 bottom center of tank body is equipped with magnetic agitation mechanism 15, and magnetic agitation mechanism 15 is connected with position
The top of agitating shaft 3 inside tank body 1, agitating shaft 3 is equipped with upper blade 4, and agitating shaft 3 is equipped with distributes alternately from top to bottom successively
The first rabbling mechanism 5 and the second rabbling mechanism.First rabbling mechanism 5 includes the fixed ring 51 for being fixed on agitating shaft 3, fixed ring
51 are connected with several first horizontal axis 52, and the end of the first horizontal axis 52 is equipped with the first blade 53；Second rabbling mechanism includes and stirring
The end of the second affixed horizontal axis 6 of axis 3, the second horizontal axis 6 is equipped with transverse slat 7, and transverse slat 7 is connected with the second blade 8, the second horizontal axis 6
Length is longer than the length of the first horizontal axis 52.The inner wall of tank body 1 is equipped with baffle 9, is matched with rabbling mechanism, realizes low-shearing force
The uniform mixing of efficient oxygen transfer, cell and nutriment off field.
Shown in as shown in Figure 1, Figure 3 and Figure 4,3 lower section of agitating shaft is equipped with microvesicle ring, and microvesicle ring gradually becomes including diameter from top to bottom
Hollow point of ring of small at least two, the present embodiment divides the quantity of ring to be 2, including upper ring 13 and lower ring 14, upper ring 13 and lower ring 14
Between with the first air intake lever 12 be connected, the first air intake lever is connected and is connected with the second air intake lever 11 between 12 and lower air inlet 10, upper ring 13
It is equipped with several equally distributed stomatas 17 with the lower surface of lower ring 14, a diameter of 0.1 millimeter of stomata 17, two neighboring stomata
17 distance is 1 centimetre.Upper ring 13 and lower ring 14 are with circular rings jaggy, and two ends are equipped with plug 18, block up
First 18 with divide ring end be is threadedly coupled, convenient disassembly, convenient for microvesicle ring cleaning and maintenance.First air intake lever 12 and second
It is connected with silk mouth nut 16 between air intake lever 11, convenient disassembly；First air intake lever 12 is in 30 degree angles with interplanar, act on be by
Microvesicle ring is placed in below rabbling mechanism, and does not have scraping collision with rabbling mechanism.
As shown in figure 5, magnetic agitation mechanism 15 includes motor 151, outer transmission shaft 152 and the intermediate solid drive shaft being sequentially connected
156, opposite outer magnetic assembly 154 and interior magnetic assembly 153, intermediate solid drive shaft 156 are equipped between outer transmission shaft 152 and intermediate solid drive shaft 156
It is connected with the bottom end of agitating shaft 3, power transmits the electromagnetic force for fully relying on magnet steel generation, and mixing effect is good, and is not easy microbiological contamination.
Magnetic agitation mechanism 15 and the joint of tank body 1 are equipped with separation sleeve 155, advantageously ensure that the gnotobasis in tank body 1.
The present invention advantage be：
(1) the first rabbling mechanism and the second rabbling mechanism that agitating shaft of the invention is equipped with upper blade and distributes alternately,
Upper blade can be stirred the liquid above tank body, and the first rabbling mechanism can be stirred the liquid at tank body center, and second
Rabbling mechanism can be stirred the liquid near inner tank wall, to realize in tank body the uniform of cell everywhere and nutriment
Mixing.
(2) present invention is equipped with microvesicle ring, and microvesicle ring includes the gradually smaller several hollow point of rings of diameter from top to bottom, can
It ventilates from different height and all directions to the cell of tank body, stomata is set to the lower surface for dividing ring, and cell can be prevented broken
Air-hole blockage can be met the cell culture of 7500L large volume tank bodies by piece, to greatly improve yield, also be improved
Product quality.
(3) present invention hits exactly magnetic agitation using bottom, and sealing effect is good, reduces microbiological contamination risk, is conducive to cell
Smoothly culture.
The foregoing is only a preferred embodiment of the present invention, is not intended to restrict the invention, although with reference to aforementioned reality
Applying example, invention is explained in detail, for those skilled in the art, still can be to aforementioned each implementation
Technical solution recorded in example is modified or equivalent replacement of some of the technical features.All essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (9)
1. a kind of biological reactor for cell culture, which is characterized in that including tank body, the top of the tank body is equipped with enterprising gas port,
Lower section is equipped with lower air inlet, and the tank base center is equipped with magnetic agitation mechanism, and the magnetic agitation mechanism, which is connected with, to be located at
The top of the agitating shaft of the tank interior, the agitating shaft is equipped with upper blade, and the agitating shaft is equipped with phase successively from top to bottom
Between the first rabbling mechanism and the second rabbling mechanism that are distributed；
First rabbling mechanism includes the fixed ring for being fixed on the agitating shaft, and it is horizontal that the fixed ring is connected with several first
Axis, the first horizontal the tip of the axis are equipped with the first blade；Second rabbling mechanism includes affixed with the agitating shaft second
Horizontal axis, the second horizontal the tip of the axis are equipped with transverse slat, and the transverse slat is connected with the second blade, and the length of second horizontal axis is longer than
The length of first horizontal axis；
Microvesicle ring is equipped with below the agitating shaft, the microvesicle ring is hollow including gradually smaller at least two of diameter from top to bottom
Divide the first air intake lever for dividing ring described in ring and connection, with the second air intake lever phase between first air intake lever and the lower air inlet
Connection, the lower surface for dividing ring are equipped with several equally distributed stomatas.
2. a kind of biological reactor for cell culture according to claim 1, which is characterized in that described that ring is divided to be with jagged
Circular rings, two ends for being divided to ring are equipped with dismountable plug.
3. a kind of biological reactor for cell culture according to claim 2, which is characterized in that the plug divides ring with described
End be threadedly coupled.
4. a kind of biological reactor for cell culture according to claim 1, which is characterized in that first air intake lever and institute
It states and is connected with silk mouth nut between the second air intake lever, first air intake lever is in 30 degree of angles with interplanar.
5. a kind of biological reactor for cell culture according to claim 1, which is characterized in that the stomata it is a diameter of
0.1 millimeter, the distance of the two neighboring stomata is 1 centimetre.
6. a kind of biological reactor for cell culture according to claim 1, which is characterized in that the height of the enterprising gas port
Higher than 50 centimetres of liquid level in the tank body.
7. a kind of biological reactor for cell culture according to claim 1, which is characterized in that magnetic agitation mechanism packet
The motor being sequentially connected, outer transmission shaft and intermediate solid drive shaft are included, is equipped between the outer transmission shaft and the intermediate solid drive shaft opposite outer
Magnetic assembly and interior magnetic assembly, the intermediate solid drive shaft are connected with the bottom end of the agitating shaft.
8. a kind of biological reactor for cell culture according to claim 1, which is characterized in that the magnetic agitation mechanism with
The joint of the tank body is equipped with separation sleeve.
9. a kind of biological reactor for cell culture according to claim 1, which is characterized in that set on the inner wall of the tank body
There is baffle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810475273.XA CN108410734A (en) | 2018-05-17 | 2018-05-17 | A kind of biological reactor for cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810475273.XA CN108410734A (en) | 2018-05-17 | 2018-05-17 | A kind of biological reactor for cell culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108410734A true CN108410734A (en) | 2018-08-17 |
Family
ID=63139908
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810475273.XA Pending CN108410734A (en) | 2018-05-17 | 2018-05-17 | A kind of biological reactor for cell culture |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115895887A (en) * | 2022-12-13 | 2023-04-04 | 成都康华生物制品股份有限公司 | Influenza vaccine production device and method and influenza vaccine |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110312087A1 (en) * | 2009-02-09 | 2011-12-22 | Mohsan Khan | Bioreactor for the cultivation of mammalian cells |
| JP2012044943A (en) * | 2010-08-27 | 2012-03-08 | Hitachi Plant Technologies Ltd | Culture vessel and culture device for biological cell |
| US20120070860A1 (en) * | 2009-06-05 | 2012-03-22 | Marco Jenzsch | Combination stirrer |
| CN203200277U (en) * | 2013-03-27 | 2013-09-18 | 南京江辉生物科技有限公司 | Biological reactor |
| CN206396254U (en) * | 2016-11-29 | 2017-08-11 | 肇庆绿健农业科技有限公司 | Asparagus liquid strain culture tank |
| CN107988074A (en) * | 2018-01-25 | 2018-05-04 | 吉林冠界生物技术有限公司 | The full suspension cell culture reactor of 6000L serum-frees |
| CN208362367U (en) * | 2018-05-17 | 2019-01-11 | 江苏弗洛瑞生物工程设备有限公司 | A kind of biological reactor for cell culture |
-
2018
- 2018-05-17 CN CN201810475273.XA patent/CN108410734A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110312087A1 (en) * | 2009-02-09 | 2011-12-22 | Mohsan Khan | Bioreactor for the cultivation of mammalian cells |
| US20120070860A1 (en) * | 2009-06-05 | 2012-03-22 | Marco Jenzsch | Combination stirrer |
| JP2012044943A (en) * | 2010-08-27 | 2012-03-08 | Hitachi Plant Technologies Ltd | Culture vessel and culture device for biological cell |
| CN203200277U (en) * | 2013-03-27 | 2013-09-18 | 南京江辉生物科技有限公司 | Biological reactor |
| CN206396254U (en) * | 2016-11-29 | 2017-08-11 | 肇庆绿健农业科技有限公司 | Asparagus liquid strain culture tank |
| CN107988074A (en) * | 2018-01-25 | 2018-05-04 | 吉林冠界生物技术有限公司 | The full suspension cell culture reactor of 6000L serum-frees |
| CN208362367U (en) * | 2018-05-17 | 2019-01-11 | 江苏弗洛瑞生物工程设备有限公司 | A kind of biological reactor for cell culture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115895887A (en) * | 2022-12-13 | 2023-04-04 | 成都康华生物制品股份有限公司 | Influenza vaccine production device and method and influenza vaccine |
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