CN207958352U - Microvesicle air-breather and system - Google Patents
Microvesicle air-breather and system Download PDFInfo
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- CN207958352U CN207958352U CN201820130740.0U CN201820130740U CN207958352U CN 207958352 U CN207958352 U CN 207958352U CN 201820130740 U CN201820130740 U CN 201820130740U CN 207958352 U CN207958352 U CN 207958352U
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Abstract
The utility model is related to zooblast bioreactor field of engineering technology, in particular to a kind of microvesicle air-breather and system.The microvesicle air-breather is with not closed circle ring jaggy, and there are one air inlet and several equally distributed micropores for setting on ring wall；The ring body inner hollow of the microvesicle air-breather, the micropore are communicated through ring wall with the hollow cavity of the ring body.The microvesicle aerating system includes bioreactor tank body, agitating device and microvesicle air-breather as described above.Since the country is before always without cultivating the precedent of cell using large capacity bioreactor (3000L~6000L), thus lack the optimization and adjustment for big volume bioreactor equipment in the prior art.Present utility model application people adapts to transformation stirring system and also reduces the dosage of oxygen to realize the uniform gas distribution of big volume bioreactor, improve the motility rate of cell by adjusting air-breather.
Description
Technical field
The utility model is related to zooblast bioreactor field of engineering technology, logical in particular to a kind of microvesicle
Device of air and system.
Background technology
With the continuous development of biovaccine industry for animals, the especially full suspension culture techniques of bioreactor cell are answered
With people start growing interest high cell densities, high quality, large volume culture.Thus in cell cultivation process gas supply
Studied major tasks are cultivated as cell amplification.Microvesicle air-breather is as traditional cell culture reactor inlet duct
It is widely used.
Existing microvesicle air-breather is mostly club shaped structure, and oxygen is broken into microvesicle by the way that porous structure is arranged, but
The device oxygen diffuser efficiency is low, and it is excessively high to be easy to cause oxygen dispersion unevenness, local oxygen concentration in reactor；In addition, porous
Structure is also unfavorable for dismantling and clean, and is easy to block after a period of use.
Stirring reactor persistently stirs evenly distribution nutrition, gas (oxygen etc.) and heat by agitating paddle, is
Object cell provides metastable growing environment.Although existing stirring reactor stirring structure is simple, oxygen is by stirring
After mixing soon will on float on reactor liquid level surface discharge, be unfavorable for making full use of for oxygen；Existing bioreactor
Stirring efficiency is low, is also unfavorable for nutrition and the dispersion of gas；In addition, big volume bioreactor (3000L~6000L) into
Row cell culture chamber, gas and nutriment are difficult to be uniformly mixed, thus need agitating paddle provide bigger rotating speed, but so with
Carrying out Cell viability can also be greatly affected.
In view of this, special propose the utility model.
Utility model content
The utility model is related to a kind of microvesicle air-breather, the microvesicle air-breather is with not closed circle jaggy
Ring, there are one air inlet and several equally distributed micropores for setting on ring wall；Inside the ring body of the microvesicle air-breather
Hollow, the micropore is communicated through ring wall with the hollow cavity of the ring body.
The microvesicle air-breather is easy to clean, is not easy to be blocked by cell fragment, and has better gas distribution effect.
Preferably, microvesicle air-breather as described above, the one of plane where the micropore is distributed in the circular rings
Side；
Preferably, the micropore is arranged along the ring wall at a row；It is furthermore preferred that the micropore is ventilated apart from the microvesicle
The distance in the device center of circle is equal.
Preferably, microvesicle air-breather as described above, the air inlet are oppositely arranged with the notch, the air inlet
It is fixed on bioreactor bottom by attachment device.
Preferably, microvesicle air-breather as described above, the air inlet are hollow by attachment device connection one
Connecting rod is fixed on bioreactor bottom；
Preferably, the connecting rod is the L-shaped structure of middle bent；It is furthermore preferred that the angle of the connecting rod middle bent
Degree is 120 °~160 °.
Preferably, the ring body of microvesicle air-breather as described above, the microvesicle air-breather is detachably two semi-rings；
Preferably, the ring body dismounting of the microvesicle air-breather is that be set to the air inlet attached for the detachment points of two semi-rings
Closely；It is furthermore preferred that the attachment device is three-way device, one end communicates with the connecting rod, in addition both ends then respectively with two
Semi-ring communicates, and is flexibly connected at least one of two semi-rings.
Preferably, microvesicle air-breather as described above, the ring body end of the microvesicle air-breather indentation, there are that can beat
The structure opened；It is furthermore preferred that the ring body end set of the microvesicle air-breather indentation, there has dismountable plug.
The utility model further relates to a kind of microvesicle aerating system comprising bioreactor tank body, agitating device and such as
The upper microvesicle air-breather.
The system can coordinate the microvesicle air-breather, further increase the dissolved oxygen amount in bioreactor, reduce oxygen
Gas consumption, gas distribution is more uniform, and cell can be made to keep 96% or more motility rate proper splitting proliferation, is very suitable for great Rong
The use of long-pending bioreactor.
Preferably, microvesicle aerating system as described above, the agitating device include agitating shaft and agitating paddle；
The agitating shaft is perpendicular to horizontal plane and is fixed on tank bottom, and the agitating paddle is set on the agitating shaft, described
The bottom of agitating shaft is connected with power plant；
The microvesicle air-breather is set to the underface of the agitating paddle, around the agitating shaft；Preferably, described micro-
The micropore of bubble air-breather is arranged downward.
Preferably, microvesicle aerating system as described above, there are three the agitating paddles, from the agitating shaft from top to bottom according to
Secondary is the first agitating paddle, the second agitating paddle and third agitating paddle；The second agitating paddle agitated liquid moves from top to bottom；Institute
The first and third agitating paddle agitated liquid is stated to move from bottom to top；
Preferably, the agitating paddle is turbine type agitating paddle or pusher agitating paddle；
It is furthermore preferred that the agitating paddle is four leaf push type agitating paddles.
Preferably, microvesicle aerating system as described above is additionally provided with baffle, the baffle inside the tank skin of the tank body
Perpendicular to horizontal plane；
Preferably, the tank body is cylinder；It is furthermore preferred that the baffle has 3~5, more preferably 4, and uniformly
It is distributed on the tank skin of the tank body；
Preferably, the baffle itself and 30 °~40 ° of Grain lines where the tank body tie point partially, and each baffle is inclined
It is consistent to turn direction.
Description of the drawings
It, below will be right in order to illustrate more clearly of specific embodiment of the present invention or technical solution in the prior art
Specific implementation mode or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, it is described below
In attached drawing be that some embodiments of the utility model are not paying creativeness for those of ordinary skill in the art
Under the premise of labour, other drawings may also be obtained based on these drawings.
The structural schematic diagram for the microvesicle air-breather that Fig. 1 is provided by the utility model one embodiment；
The schematic diagram for the microvesicle air-breather notch end amplification that Fig. 2 is provided by the utility model one embodiment；
The schematic diagram for the microvesicle air-breather inlet port amplification that Fig. 3 is provided by the utility model one embodiment；
The schematic diagram for the connecting rod that Fig. 4 is provided by the utility model one embodiment；
The bioreactor that Fig. 5 is provided by the utility model one embodiment；
The vertical view for the bioreactor that Fig. 6 is provided by the utility model one embodiment.
Reference numeral：
Bioreactor tank body A；
Microvesicle air-breather 1；Air admission hole 101；Micropore 102；Attachment device 103；Sticking department 1031；Protruding portion 1032；Even
Extension bar 104；Plug 105；
Agitating device 2；Agitating shaft 201；Agitating paddle 202；
Power plant 3；
Baffle 4.
Specific implementation mode
The technical solution of the utility model is clearly and completely retouched below in conjunction with the drawings and specific embodiments
It states, it will be appreciated by those skilled in the art that following described embodiment is the utility model a part of the embodiment, without
It is whole embodiment, is merely to illustrate the utility model, and is not construed as limitation the scope of the utility model.Based on this practicality
Embodiment in novel, every other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, shall fall within the protection scope of the present invention.The person that is not specified actual conditions in embodiment, according to normal condition or manufacturer
It is recommended that condition carry out.Reagents or instruments used without specified manufacturer is the routine that can be obtained by commercially available purchase
Product.
It is in the description of the present invention, it should be noted that term "center", "upper", "lower", "left", "right", " perpendicular
Directly ", the orientation or positional relationship of the instructions such as "horizontal", "inner", "outside" is to be based on the orientation or positional relationship shown in the drawings, and is only
The utility model and simplifying describes for ease of description, do not indicate or imply the indicated device or element must have it is specific
Orientation, with specific azimuth configuration and operation, therefore should not be understood as limiting the present invention.In addition, term " the
One ", " second ", " third " are used for description purposes only, and are not understood to indicate or imply relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " connected ", " connection " shall be understood in a broad sense, for example, it may be being fixedly connected, may be a detachable connection, or integrally
Connection；It can be mechanical connection, can also be electrical connection；Can be directly connected, can also indirectly connected through an intermediary,
It can be the connection inside two elements.For the ordinary skill in the art, it can understand above-mentioned art with concrete condition
The concrete meaning of language in the present invention.
The utility model is related to a kind of microvesicle air-breathers, as shown in Figure 1, the microvesicle air-breather 1 is with jagged
Not closed circle ring；On the ring wall of the microvesicle air-breather 1 setting there are one air inlet 101 and several be uniformly distributed
Micropore 102；The ring body inner hollow of the microvesicle air-breather, the micropore 102 are hollow through ring wall and the ring body
Cavity communicates.
Preferably, in the microvesicle air-breather 1 in use, the gas that the air inlet 101 is passed through is oxygen, dioxy
Change carbon, filtrated air or this field and carry out other common gases when cell culture, preferably above-mentioned gas is compressed gas.
The air being passed through constitutes the deep layer gas handling system of cell culture reactor, which, which is passed through mode, to prolong
The flow path of long air in the medium increases the transfer amount of carbon dioxide and ammonia.
Preferably, the pressure of compressed air is 0.02~0.06MPa, more preferably 0.04MPa, and the compressed air passes through
Cross the filtering of two-stage air filter.
It is carried out in the microvesicle air-breather 1 in use, being injected gas such as oxygen by the air admission hole 101, one
Under fixed air pressure, oxygen is full of entire microvesicle air-breather by the ring body of inner hollow, and is discharged by the micropore 102.The dress
Set simple in structure, thus micropore is not easy to plug, and is safeguarded also more convenient.The structure of annular increases gas distribution area, can more
It is even that oxygen is released in cell culture fluid.
Preferably, a diameter of 0.05mm~0.2mm of the micropore 102.
For the residence time that can increase using oxygen when the device in culture solution as much as possible, it is preferred that described micro-
The side of plane where hole 102 is distributed in the circular rings, in this way in use, can be by setting increases down where micropore 102
The advanced distance of oxygen floating.
According to ventilation demand needed for, the micropore 102 can according to S-shaped or it is Z-shaped arranged, may be set to be more
Row.In order to reduce technology difficulty, it is preferred that the micropore 102 is arranged along the ring wall at a row；It is furthermore preferred that the micropore
102 distance apart from 1 center of circle of microvesicle air-breather is equal.
Preferably, microvesicle air-breather as described above, the air inlet 101 are oppositely arranged with the notch, it is described into
Gas port 101 is fixed on bioreactor bottom by attachment device 103.
Preferably, microvesicle air-breather as described above, the air inlet 101 connect one by the attachment device 103
A hollow connecting rod 104 is fixed on bioreactor bottom；
Preferably, as shown in figure 3, the connecting rod 104 is the L-shaped structure of middle bent；It is furthermore preferred that the connecting rod
The angle of 104 middle bents is 120 °~160 °；It can also select 130 °~150 ° or 140 °.
The angle of 104 middle bent of connecting rod is 120 °~160 °, i.e., when one section is connected with the microvesicle air-breather,
The other end can horizontal plane upwarped in 20 °~60 ° angles, effect be by microvesicle air-breather as agitating paddle lower section, and not with stir
Mixing paddle has scraping collision.104 other side of connecting rod and reactor can silk mouth nut be connected, convenient disassembly.
The microvesicle air-breather 1 is cleaned for convenience, it is preferred that the ring body of the microvesicle air-breather 1 can
Dismounting is two semi-rings；Cleaning way can be to be cleaned by ultrasonic.
In order to preferably simplify structure, it is preferred that the ring body dismounting of the microvesicle air-breather 1 is two semi-rings
Detachment point is set near the air inlet；It is furthermore preferred that as shown in Fig. 2, the attachment device 103 be three-way device, one end
It is communicated with the connecting rod 104, in addition both ends are then communicated with two semi-rings respectively, and movable at least one of two semi-rings
Connection.Active connection place can be attached by a connector, as shown in Fig. 2, the connector is by sticking department 1031 and protrudes
Portion 1032 forms；The two can be connected by the form of lock, may be alternatively provided as magnetic connection.
Preferably, microvesicle air-breather as described above, the ring body end of 1 indentation, there of microvesicle air-breather are that can beat
The structure opened；It is furthermore preferred that as shown in figure 4, the ring body end set of the microvesicle air-breather indentation, there has dismountable block up
First 105.
Dismountable plug is also conducive to the cleaning of microvesicle air-breather.
In an embodiment of the utility model, the circle diameter of the microvesicle air-breather 1 is 570mm, notch
The length at place is 80mm, and 102 spacing of micropore has 2.5mm, 102 a diameter of 0.05mm~0.2mm of micropore.
The utility model further relates to a kind of microvesicle aerating system, as shown in Figure 5 comprising bioreactor tank body A, stirring
Device 2 and microvesicle air-breather 1 as described above.
Microvesicle air-breather provided by the utility model, due to the gas distribution area with bigger, thus it is preferred, it is described
Bioreactor is the bioreactor of big volume, the especially bioreactor of 3000L~6000L.
Preferably, microvesicle aerating system as described above, the agitating device 2 include agitating shaft 201 and agitating paddle 202；
The agitating shaft 201 is perpendicular to horizontal plane and is fixed on tank bottom, and the agitating paddle 202 is set to the agitating shaft
On 201, the bottom of the agitating shaft 201 is connected with power plant 3；
The microvesicle air-breather 1 is set to the underface of the agitating paddle 202, around the agitating shaft 201；It is preferred that
, the micropore 102 of the microvesicle air-breather 1 is arranged down.
Preferably, the power plant 3 is magnetic stirring apparatus.
Zooblast reactor is very strict to sterility requirements, operations and the mechanically-sealing apparatus such as charging, sterilizing and inoculation
Deng may all bring microbiological contamination risk, once leading to inside reactor microbiological contamination, huge economic loss is caused.The utility model is developed
There is the magnetic drives stirring system of completely isolated function, power for the bioreactor of 6000L animal cell cultures
The electromagnetic force for fully relying on the generation of magnetic cylinder is transmitted, the microbiological contamination problem caused by sealing has thoroughly been prevented.
The microvesicle air-breather 1 is inserted in by its notch around the agitating shaft 201.
Preferably, microvesicle aerating system as described above, there are three the agitating paddles 202, from the agitating shaft by up to
Under be followed successively by the first agitating paddle, the second agitating paddle and third agitating paddle, the second agitating paddle agitated liquid is transported from top to bottom
It is dynamic；The first and third agitating paddle agitated liquid moves from bottom to top.
By above-mentioned set-up mode, the oxygen discharged in microvesicle air-breather 1 can be confined between the first and third agitating paddle
(it is considered that spiral liquid stream is formd between three agitating paddles), it is resident in culture solution to extend bubbles of oxygen
Time increases the utilization rate of oxygen.
Preferably, the agitating paddle 202 is turbine type agitating paddle or pusher agitating paddle；
The blade of pusher agitating paddle has certain radian, and culture solution is enabled to form axial flowing, is stirred to mixing
It is more advantageous with suspension effect.
It is furthermore preferred that the agitating paddle 202 is four leaf push type agitating paddles.
Preferably, the rake angle degree of the agitating paddle is 40 °~60 °；More preferably 50 °；Since each agitating paddle makes
The direction of motion of liquid stream is different, thus the deflection of the deflection angle and first and third agitating paddle of preferred second agitating paddle
Angle mirror symmetry.
Preferably, the ratio of the paddle diameter of the agitating paddle and tank diameter is 0.2~0.7, more preferably 0.3~0.5.
Preferably, the rotating speed of the agitating paddle is 30~50rpm.
Preferably, microvesicle aerating system as described above is additionally provided with baffle 4, the gear inside the tank skin of the tank body A
Plate 4 is perpendicular to horizontal plane；
It is furthermore preferred that the bottom of the baffle 4 is concordant with the third agitating paddle, top is higher than the liquid level of cell culture fluid
Highly.
Preferably, the tank body is cylinder；It is furthermore preferred that the baffle 4 has 3~5, more preferably 4, and uniformly
It is distributed on the tank skin of the tank body；
Preferably, as shown in fig. 6, the baffle 4 deviate its with 30 ° of Grain lines where the tank body A tie points~
40 °, and each baffle deflection direction is consistent；
Preferably, the width of the baffle is 1/13~1/10 tank diameter.
It is furthermore preferred that each baffle deflection direction is consistent with the direction of rotation of the first and third agitating paddle；Such as described
One, the direction of rotation of three agitating paddles is counterclockwise, then the deflection direction of the baffle 4 is just as shown in fig. 6, along flow counterclockwise
It deflects in obtuse angle.
Due to second agitating paddle and first and third agitating paddle by the direction of aqueous agitation on the contrary, culture solution into
When row agitation liquid flow direction can than three agitating paddles equidirectional (upwards agitation) it is more chaotic, therefore, this agitation mode
Although dissolved oxygen amount can be greatly increased, it is also easier to, because the chaotic impact of liquid stream forms turbulent flow, it is broken in turn result in cell simultaneously
Broken death.Based on this reason, the utility model is provided with baffle 4, and its deflection angle is consistent with the direction of rotation of agitating paddle, gear
Plate 4 can play the role of water conservancy diversion, since cell culture fluid closes on flow velocity maximum at tank skin in bioreactor, thus on tank skin
Baffle 4 can slow down flow rate of liquid, guiding liquid flow direction herein, and then effectively avoid the generation of turbulent flow.
For example, in one embodiment, bioreactor tank body is 6000L tank bodies, microvesicle air-breather used is Fig. 1
Shown in circular air-breather.As shown in figure 5, bottom is provided with agitating device in tank, the agitating device includes stirring
Axis and agitating paddle, the agitating shaft is perpendicular to horizontal plane and is fixed on tank bottom, and the agitating paddle is set on the agitating shaft, institute
The bottom for stating agitating shaft is connected with power plant；Three agitating paddles are set on agitating shaft, and rotating speed is 40 turns/min, is the inverse time
Needle rotates.The second agitating paddle agitated liquid moves from top to bottom；The first and third agitating paddle agitated liquid is transported from bottom to top
It is dynamic.The rake angle degree of agitating paddle is 50 °, and the ratio of paddle diameter and tank diameter is 0.4.Four baffles are set on tank skin, baffle
It is to deviate itself and 35 ° of Grain lines where the tank body tie point counterclockwise to deflect direction, and the width of baffle is 1/12 tank diameter.
It sets this embodiment to experimental group, comparative example is set on this basis；
Comparative example 1：It is differed only in experimental group, removes baffle.
Comparative example 2：It is differed only in experimental group, baffle is arranged perpendicular to tank body.
Comparative example 3：It is differed only in experimental group, three agitating paddles stir culture solution upwards, and remove baffle.
Comparative example 4：It is differed only in experimental group, which is the cylinder of routine, and inside has porous
Reticular structure.
Cultivated cell is mdck cell, is after 1,800,000/ml is cultivated 5 days, to detect following data by cell culture density：
Note：Cell survival rate=(total number of cells-dead cell number) ÷ total number of cells × 100%, trypan blue staining meter
It calculates.
From comparative example 1 and 2 it is found that baffle plate setting mode it is incorrect or removal baffle, although flow of oxygen can be reduced,
More violent turbulent flow can be formed, Cell viability is caused to be greatly lowered.By comparative example 3 it is found that three agitating paddles will culture
When liquid stirs upwards, it is resident and is shortened in culture solution due to oxygen, thus oxygen utilization rate declines, oxygen consumption total amount is soaring apparent；
It is uniformly dispersed in addition, the change of aqueous agitation mode is also unfavorable for oxygen, it is possible to which the decline for causing local oxygen concentration causes
Cell viability reduces.By comparative example 4 it is found that routine microvesicle air-breather in the prior art, since gas distribution is uneven, same meeting
Cell viability is reduced, and since the decline of oxygen dissolved efficiency leads to the raising of flow of oxygen.Further, since the prior art is adopted
Conventional microvesicle air-breather not easy cleaning, can be blocked in the normal time using rear micropore, if increasing bleed pressure at this time,
It is too fast that the ventilation gas flow hole rate of outflow for causing not block is returned again, gas effciency is caused to reduce, and is especially reacted in 6000L
When device culture cell, under the maximum mixing speed for not damaging cell microvesicle air inlet can only supply the dissolved oxygen of culture 0-20%
Amount, is unable to reach the dissolved oxygen amount of the 30-50% needed for cell growth.
In conclusion becoming larger with overall volume, cell cloth oxygen difficulty skyrockets.Due to always not used before the country
The precedent of big volume bioreactor (3000L~6000L) culture cell, thus lack in the prior art for big volume biology
The optimization and adjustment of reactor apparatus.Present utility model application people adapts to transformation stirring system by adjusting air-breather, from
And the uniform gas distribution of big volume bioreactor is realized, the dosage of oxygen is also reduced, the survival rate of cell is improved.
Finally it should be noted that：The above various embodiments is only to illustrate the technical solution of the utility model, rather than limits it
System；Although the utility model has been described in detail with reference to the foregoing embodiments, but those skilled in the art answer
Work as understanding：It still can be with technical scheme described in the above embodiments is modified, either to which part or entirely
Portion's technical characteristic carries out equivalent replacement；And these modifications or replacements, this practicality that it does not separate the essence of the corresponding technical solution
The range of novel each embodiment technical solution.
Claims (10)
1. a kind of microvesicle air-breather, which is characterized in that the microvesicle air-breather is with not closed circle ring jaggy, ring
There are one air inlet and several equally distributed micropores for setting on wall；The ring body inner hollow of the microvesicle air-breather,
The micropore is communicated through ring wall with the hollow cavity of the ring body.
2. microvesicle air-breather according to claim 1, which is characterized in that the micropore is distributed in the circular rings institute
In the side of plane.
3. microvesicle air-breather according to claim 1, which is characterized in that the air inlet is opposite with the notch to be set
It sets, the air inlet is fixed on bioreactor bottom by attachment device.
4. microvesicle air-breather according to claim 3, which is characterized in that the air inlet is connected by the attachment device
It connects a hollow connecting rod and is fixed on bioreactor bottom.
5. microvesicle air-breather according to claim 4, which is characterized in that the ring body of the microvesicle air-breather is detachable
For two semi-rings.
6. microvesicle air-breather according to claim 1, which is characterized in that the ring body of the microvesicle air-breather indentation, there
End is openable structure；It is furthermore preferred that the ring body end set of the microvesicle air-breather indentation, there has dismountable block up
Head.
7. a kind of microvesicle aerating system comprising any one of bioreactor tank body, agitating device and claim 1~6 institute
The microvesicle air-breather stated.
8. microvesicle aerating system according to claim 7, which is characterized in that the agitating device includes agitating shaft and stirring
Paddle；
The agitating shaft is perpendicular to horizontal plane and is fixed on tank bottom, and the agitating paddle is set on the agitating shaft, the stirring
The bottom of axis is connected with power plant；
The microvesicle air-breather is set to the underface of the agitating paddle, around the agitating shaft；Preferably, the microvesicle is logical
The micropore of device of air is arranged downward.
9. microvesicle aerating system according to claim 8, which is characterized in that there are three the agitating paddles, from the stirring
Axis is from top to bottom followed successively by the first agitating paddle, the second agitating paddle and third agitating paddle；The second agitating paddle agitated liquid by
On move downward；The first and third agitating paddle agitated liquid moves from bottom to top.
10. according to claim 7~9 any one of them microvesicle aerating system, which is characterized in that inside the tank skin of the tank body
It is additionally provided with baffle, the baffle is perpendicular to horizontal plane；
The tank body is cylinder；The baffle has 3~5, and is uniformly distributed on the tank skin of the tank body；
The baffle deviates itself and 30 °~40 ° of Grain lines where the tank body tie point, and each baffle deflects direction one
It causes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820130740.0U CN207958352U (en) | 2018-01-25 | 2018-01-25 | Microvesicle air-breather and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201820130740.0U CN207958352U (en) | 2018-01-25 | 2018-01-25 | Microvesicle air-breather and system |
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| Publication Number | Publication Date |
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| CN207958352U true CN207958352U (en) | 2018-10-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034575A (en) * | 2018-01-25 | 2018-05-15 | 吉林冠界生物技术有限公司 | Microvesicle air-breather and system |
-
2018
- 2018-01-25 CN CN201820130740.0U patent/CN207958352U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108034575A (en) * | 2018-01-25 | 2018-05-15 | 吉林冠界生物技术有限公司 | Microvesicle air-breather and system |
| CN108034575B (en) * | 2018-01-25 | 2024-02-06 | 吉林冠界生物技术有限公司 | Microbubble ventilation device and system |
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