CN204325379U - A kind of microcarrier and cell culture apparatus - Google Patents
A kind of microcarrier and cell culture apparatus Download PDFInfo
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- CN204325379U CN204325379U CN201420856587.1U CN201420856587U CN204325379U CN 204325379 U CN204325379 U CN 204325379U CN 201420856587 U CN201420856587 U CN 201420856587U CN 204325379 U CN204325379 U CN 204325379U
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- microcarrier
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- culture
- culture apparatus
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Abstract
The utility model relates to a kind of microcarrier and cell culture apparatus, and its inner culturing cell can repeatedly be changed, and saves production cost.This kind of microcarrier and cell culture apparatus, comprise cylindrical culture vessel, and the top of this culture vessel is provided with liquid outlet, and bottom is provided with fluid inlet, and this culture vessel is divided into separable two portions, is connected between these two portions by joint flange with fixture; The inner chamber of this culture vessel is provided with at least one deck screen plate.Preferably, described fixture is divided into four, and the circumference along described joint flange is evenly arranged.
Description
Technical field
The utility model relates to a kind of culture apparatus, particularly the culture apparatus of a kind of microcarrier and cell.
Background technology
At present, attached cell large scale culturing technology is vaccine expanding production core key technology.Microcarrier has high specific surface-area in all adherent culture containers, when forefathers use and live vaccine new generation vaccine more options microcarrier suspension culture technology.Microcarrier suspension culture technology has several difficult point to need to overcome: inoculating cell and microcarrier are adherent for the first time, owing to being suspended state, cell and microcarrier adherent rate instability, the cells on microcarriers often had is a lot, and the microcarrier yet had there is no cell, the microcarrier late stage of culture causing cell many does not have more area cell amplification altogether, and the microcarrier of blank terminates also do not have cell to cultivation.Cause inoculate uneven reason be suspension agitated conditions cell microcarrier contact and expansion time few, and stop stir, microcarrier first precipitates, and cell only contacts the microcarrier on upper strata.Device at present for microcarrier and cell cultures is a cylindrical vessel, and this container designs for integral type, and be respectively provided with liquid in-out mouth up and down, attached cell enters deep bed filter after internal tank mixes with microcarrier.This kind of device cannot be changed due to its inner cell, more can only renew container, cause to produce rising this increase after single use.In addition the cell of this internal tank can only be adherent, and often cause wall cell distribution uneven, Contact and the mixed effect of cell and microcarrier are bad.
Utility model content
For deficiency of the prior art, the utility model aims to provide the culture apparatus of a kind of detachable microcarrier and cell, and its inner culturing cell can repeatedly be changed, and saves production cost.
In order to solve the problem, this kind of microcarrier and cell culture apparatus, comprise cylindrical culture vessel, the top of this culture vessel is provided with liquid outlet, bottom is provided with fluid inlet, and this culture vessel is divided into separable two portions, is connected between these two portions by joint flange with fixture; The inner chamber of this culture vessel is provided with at least one deck screen plate.
Preferably, described fixture is divided into four, and the circumference along described joint flange is evenly arranged.
The beneficial effects of the utility model are: because culture vessel can be divided into two, replacing recycles culturing cell and microcarrier is called possibility, greatly save production cost, and make that screen plate is set at internal tank and become possibility, like this, cell not only can be attached on container inner wall, and can rest on screen plate, make cell distribution evenly, Contact and the mixed effect of cell and microcarrier are better.
Accompanying drawing explanation
Fig. 1 is the structural representation of microcarrier of the present utility model and cell culture apparatus;
Fig. 2 is the application structure schematic diagram of microcarrier of the present utility model and cell culture apparatus.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described further.
As shown in Figure 1, this kind of microcarrier and cell culture apparatus, comprise cylindrical culture vessel 1, the top of this culture vessel is provided with liquid outlet 1a, bottom is provided with fluid inlet 1b, this culture vessel 1 is divided into separable upper and lower two portions, is connected between two portions by respective joint flange 11 with four fixtures 12.This fixture can be evenly arranged along the circumference of joint flange 11.Because culture vessel can be divided into two, screen plate is set at internal tank and becomes possibility, at least one deck screen plate (not shown) is provided with at the inner chamber of culture vessel 1, cell not only can be attached on container inner wall, and can rest on screen plate, make cell distribution evenly, Contact and the mixed effect of cell and microcarrier are better.
As shown in Figure 2, nutrient solution enters into the culture vessel 1 of culture apparatus of the present utility model through filter by peristaltic pump 2, microcarrier and cytomixis enter aseptic pyrogen-free BIOSD depth filter, microcarrier and cell retention are on filter plate, fresh medium infiltrating cells and microcarrier, cell adherent stretching, extension on microcarrier and filter plate after one day, starts amplification.Because culture vessel has 40 liters of volume of culture, 5 kilograms of microcarriers can be held, be equivalent to the throughput of 500 liters of microcarrier suspension culture reactors.The density of microcarrier is 10 times of common suspension culture.The expansion of culture area, seed cell is static evenly adherent, and Later growth perfusion is cultivated, and cell yield and production of vaccine ability easily increase.Afterwards nutrient solution by liquid outlet and filter flow reversal in the medium-sized reservoir 3 of storage nutrient solution.
It should be noted that; embodiment is only explanation to technical solutions of the utility model and explanation; should not be understood as the restriction to technical solutions of the utility model; any employing the technical solution of the utility model and only do local change, must fall in protection domain of the present utility model.
Claims (2)
1. a microcarrier and cell culture apparatus, comprise cylindrical culture vessel (1), the top of this culture vessel is provided with liquid outlet (1a), bottom is provided with fluid inlet (1b), it is characterized in that: this culture vessel (1) is divided into separable two portions, be connected with fixture (12) by joint flange (11) between these two portions; The inner chamber of this culture vessel (1) is provided with at least one deck screen plate.
2. microcarrier according to claim 1 and cell culture apparatus, is characterized in that: described fixture (12) is divided into four, and the circumference along described joint flange (11) is evenly arranged.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420856587.1U CN204325379U (en) | 2014-12-30 | 2014-12-30 | A kind of microcarrier and cell culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420856587.1U CN204325379U (en) | 2014-12-30 | 2014-12-30 | A kind of microcarrier and cell culture apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN204325379U true CN204325379U (en) | 2015-05-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201420856587.1U Active CN204325379U (en) | 2014-12-30 | 2014-12-30 | A kind of microcarrier and cell culture apparatus |
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| CN (1) | CN204325379U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109943471A (en) * | 2017-12-21 | 2019-06-28 | 北京中原合聚经贸有限公司 | A kind of mescenchymal stem cell filtering perfusion culture preparation facilities |
| CN111433343A (en) * | 2017-11-29 | 2020-07-17 | 康宁股份有限公司 | Filtered cell culture cover and cell culture method |
| CN111979088A (en) * | 2019-05-23 | 2020-11-24 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
| CN114317395A (en) * | 2021-12-31 | 2022-04-12 | 中国肉类食品综合研究中心 | Adherent cell large-scale culture process |
-
2014
- 2014-12-30 CN CN201420856587.1U patent/CN204325379U/en active Active
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111433343A (en) * | 2017-11-29 | 2020-07-17 | 康宁股份有限公司 | Filtered cell culture cover and cell culture method |
| CN109943471A (en) * | 2017-12-21 | 2019-06-28 | 北京中原合聚经贸有限公司 | A kind of mescenchymal stem cell filtering perfusion culture preparation facilities |
| CN111979088A (en) * | 2019-05-23 | 2020-11-24 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
| CN111979088B (en) * | 2019-05-23 | 2022-08-26 | 比欧联科供应链管理(北京)有限公司 | Breathable bag forward and reverse perfusion cell culture device and culture method |
| CN114317395A (en) * | 2021-12-31 | 2022-04-12 | 中国肉类食品综合研究中心 | Adherent cell large-scale culture process |
| CN114317395B (en) * | 2021-12-31 | 2024-04-02 | 中国肉类食品综合研究中心 | Large-scale culture process for adherent cells |
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Address after: Room 308, Zone C, floor 3, Xinya building, No. 63, Xisihuan North Road, Haidian District, Beijing 100097 Patentee after: Beijing Zhongyuan Heju Biotechnology Co., Ltd. Address before: 100125 No. 11 Dongfang East Road, Beijing, Chaoyang District Patentee before: BEIJING ZHONGYUAN HEJU ECONOMIC AND TRADE CO., LTD. |
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| TR01 | Transfer of patent right |
Effective date of registration: 20220107 Address after: 101300 room 413-414, 4th floor, building 2, yard 4, Jinhang West Road, Shunyi District, Beijing (Tianzhu comprehensive free trade zone) Patentee after: BYUCO Supply Chain Management (Beijing) Co.,Ltd. Address before: Room 308, Zone C, third floor, Xinya building, No. 63, West Fourth Ring North Road, Haidian District, Beijing 100097 Patentee before: Beijing Zhongyuan Heju Biotechnology Co.,Ltd. |