CN203399586U - Peony tissue culture flask - Google Patents
Peony tissue culture flask Download PDFInfo
- Publication number
- CN203399586U CN203399586U CN201320368867.3U CN201320368867U CN203399586U CN 203399586 U CN203399586 U CN 203399586U CN 201320368867 U CN201320368867 U CN 201320368867U CN 203399586 U CN203399586 U CN 203399586U
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- bottle
- tissue culture
- inlet
- tree peony
- row
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a peony tissue culture flask. The peony tissue culture flask comprises a flask body, a liquid discharging and feeding hole, a rock wool block, a rubber plug and a sealing film. The peony tissue culture flask disclosed by the utility model is simple to operate; peonies cultured by the peony tissue culture flask do not need to be transferred, so that labor force is saved, and the efficiency is high; therefore, the peony tissue culture flask can be widely applied to research and development institutions, factories and enterprises.
Description
Technical field
The utility model belongs to biological culture technigue field, is specifically related to a kind of tree peony tissue culture flasks.
background technology
The problems such as tree peony is candidate's national flower, and traditional modes of reproduction be take grafting as main, exists the cycle long, and reproduction coefficient is low.Utilize group culturation rapid propagating technology to produce tree peony seedling, have advantages of that speed is fast, seedling quality is neat.The following problem of existing tissue culture flasks ubiquity: (1) is in tree peony tissue culture procedures, researcher completes inoculation, the shoot proliferation of tree peony explant, the work such as take root in aseptic blake bottle, in this course, relate to the subculture switching of group training seedling.Conventionally each switching all will be changed blake bottle and culture fluid, all will carry out once sterilizing to blake bottle and the culture fluid changed, to operation, has brought very large inconvenience, has also increased cost.(2), in tree peony group training seedling switching process, easily cause brownization of group training seedling, undergrowth, even dead.(3) whole tree peony group training seedling switching process complex steps, wayward, easily pollutes.
utility model content
The utility model is in order to overcome the deficiencies in the prior art, a kind of efficient tree peony tissue culture flasks is provided, to simplify the switching step of tree peony shoot proliferation, improve the efficiency of tree peony switching, reduce brown rate, lethality and pollution rate in tree peony group training seedling switching process.
The utility model adopts following technical scheme:
A kind of tree peony tissue culture flasks that the utility model provides, comprises bottle, row's inlet, mineral wool block, sealed membrane, plug.The bottom that row's inlet is located at bottle is apart from 0.5-1cm place at the bottom of bottle.
Preferably, described bottle is that PC is that Merlon or PP are polypropylene material; Described rock wool is placed on a bottle inner bottom part, and it is irregularly shaped that rock wool is, and volume size is 0.1-1cm
3.Placement thickness is 1-2cm.
Preferably, described row's inlet is given prominence to bottle 0.5-1cm, and the bottom that described row's inlet is positioned at bottle is apart from 0.5-1cm place at the bottom of bottle, and described row's inlet is provided with plug.
Preferably, the bottleneck of described blake bottle is thread structure, and bottleneck seals with described sealed membrane, and described sealed membrane can be breathed freely.The outer gas exchange of bottle in Easy-used bottle is set like this, is conducive to a bottle interior tree peony seedling growth.
Tree peony tissue culture flasks described in the utility model, its advantage is: under (1) aseptic condition, the row's of opening inlet, effluent discharge, sterilization plug beyond the Great Wall after completing, then injects fresh medium from row's inlet, and it is convenient to change; (2) simplify switching step and improved the efficiency of tree peony switching, reduced brown rate, lethality and pollution rate in tree peony group training seedling switching process.Generally can reduce labour cost 30% left and right.(3) sealed membrane can filtered air, prevents that miscellaneous bacteria from entering in tissue culture flasks.Can prevent from polluting, can meet again bottle needs of interior tree peony group training seedling to air.Therefore, the utility model can be widely used in universities, R&D institution and factory, enterprise.
accompanying drawing explanation
Fig. 1 is the structural representation of the utility model culture medium preparing device preferred embodiment.
In figure: 1-bottle, 11-arranges inlet, 2-sealed membrane, the 21-sealed membrane filter paper of breathing freely, 3-mineral wool block, 4-feed liquor plug.
Embodiment
Below in conjunction with drawings and Examples, the utility model is done to one and describe in detail, but not as to restriction of the present utility model.
1 one kinds of tree peony tissue culture flasks of embodiment
Figure 1 shows that the structural representation of tree peony tissue culture flasks preferred embodiment of the present utility model, described culture medium preparing device comprises bottle 1, row's inlet 11, sealed membrane 2, rock wool 3, plug 4.Described row's inlet is located at bottle 1 sidewall apart from 0.5-2cm place at the bottom of bottle, and row inlet place is provided with plug 4.Described mineral wool block 3 is placed on bottle 1 inner bottom part, and it is irregularly shaped that mineral wool block is, and volume size is 0.1-1cm
3, placement thickness is 1-2cm.Described blake bottle bottle 1 for closing in PC be that Merlon or PP are polypropylene containers, bottle 1 uncovered outside, upper end is sealed by sealed membrane.
When the utility model is used in Plant Tissue Breeding laboratory, cultivation bottle 1, sealed membrane 2, mineral wool block 3 use newspapers are wrapped and carried out autoclaving, plug 4 is immersed in to thimerosal sterilizing.On aseptic superclean bench, pack mineral wool block 3 into mineral wool block 3 positions, then culture fluid is poured into and cultivated in bottle 1, by plug 4 row's of being installed to inlet 11 places.The tree peony bud clump that needs are cultivated is inoculated in mineral wool block, seals sealed membrane 2, is put on corresponding culturing rack and cultivates.Cultivate after a period of time, along with the nutrient component consumption in culture fluid, can not meet the needs of the interior tree peony group of bottle 1 training seedling growth.While changing nutrient solution, the plug 4 on only need the row of opening inlet 11, can discharge discarded culture fluid, arranges inlet 11 and plug 4 after discharging discarded culture fluid with 70% alcohol disinfecting.Culture fluid is injected in bottle 1 by row's inlet 11, and 70% alcohol disinfecting row inlet 11, installs plug 4.In changing the whole process of culture fluid, do not need to take out tree peony group training seedling, after too much culture, when tree peony group training seedling is looked prescribed level, in the time of need to again transferring, repeat above step.
Claims (4)
1. a tree peony tissue culture flasks, is characterized in that, comprises bottle, row inlet, mineral wool block, plug, sealed membrane, and the bottom that row's inlet is located at bottle is apart from 0.5-1cm place at the bottom of bottle.
2. tree peony tissue culture flasks as claimed in claim 1, is characterized in that, described bottle is that PC is that Merlon or PP are polypropylene material; Described mineral wool block is placed on a bottle inner bottom part, and it is irregularly shaped that mineral wool block is, and volume size is 0.1-1cm
3, placement thickness is 1-2cm.
3. tree peony tissue culture flasks as claimed in claim 1, is characterized in that, described row's inlet is given prominence to bottle 0.5-1cm, and the bottom that described row's inlet is positioned at bottle is apart from 0.5-1cm place at the bottom of bottle, and described row's inlet is provided with plug.
4. tree peony tissue culture flasks as claimed in claim 1, is characterized in that, described blake bottle bottleneck is thread structure, and bottleneck seals with described sealed membrane, and described sealed membrane can be breathed freely.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320368867.3U CN203399586U (en) | 2013-06-15 | 2013-06-15 | Peony tissue culture flask |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320368867.3U CN203399586U (en) | 2013-06-15 | 2013-06-15 | Peony tissue culture flask |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203399586U true CN203399586U (en) | 2014-01-22 |
Family
ID=49935536
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320368867.3U Expired - Fee Related CN203399586U (en) | 2013-06-15 | 2013-06-15 | Peony tissue culture flask |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203399586U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115530072A (en) * | 2022-09-02 | 2022-12-30 | 广西壮族自治区林业科学研究院 | Culture bottle for solving browning of African daisy culture material |
-
2013
- 2013-06-15 CN CN201320368867.3U patent/CN203399586U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115530072A (en) * | 2022-09-02 | 2022-12-30 | 广西壮族自治区林业科学研究院 | Culture bottle for solving browning of African daisy culture material |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140122 Termination date: 20140615 |
|
| EXPY | Termination of patent right or utility model |