CN209508287U - A kind of steril cell culture bottle - Google Patents
A kind of steril cell culture bottle Download PDFInfo
- Publication number
- CN209508287U CN209508287U CN201920057499.8U CN201920057499U CN209508287U CN 209508287 U CN209508287 U CN 209508287U CN 201920057499 U CN201920057499 U CN 201920057499U CN 209508287 U CN209508287 U CN 209508287U
- Authority
- CN
- China
- Prior art keywords
- bottle body
- cell culture
- bottle
- tube
- fixedly installed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 238000004113 cell culture Methods 0.000 title claims abstract description 51
- 239000013598 vector Substances 0.000 claims abstract description 16
- 239000011241 protective layer Substances 0.000 claims abstract description 13
- 239000000523 sample Substances 0.000 claims description 13
- 239000000463 material Substances 0.000 claims description 5
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000010410 layer Substances 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000000741 silica gel Substances 0.000 claims description 4
- 229910002027 silica gel Inorganic materials 0.000 claims description 4
- 239000007787 solid Substances 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 6
- 239000003344 environmental pollutant Substances 0.000 abstract description 3
- 231100000719 pollutant Toxicity 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000004500 asepsis Methods 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 238000012832 cell culture technique Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of steril cell culture bottles, including bottle body, the upper surface of the bottle body is fixedly installed with convex body, the bottom of the bottle body is equipped with groove corresponding with convex body, the surface of the bottle body is equipped with protective layer, and the inlet tube is equipped with connection parent in one end far from bottle body, and the other side of the bottle body is fixedly installed with outlet tube, the gas-guide tube is fixedly connected with miillpore filter device in one end far from bottle body, and the surface of the bottle body inner wall is fixedly installed with cell culture vector.The utility model is equipped with convex body and groove in the outside of bottle body, the inside of bottle body is equipped with several cell culture vectors, realize that single culture bottle can cultivate greater number of cell, pass through the grafting of convex body and groove, facilitate storage and places Tissue Culture Flask, it is equipped with connection parent in one end of inlet tube simultaneously, one end of gas-guide tube is equipped with miillpore filter device, prevents bacterium and pollutant from entering inside bottle body.
Description
Technical field
The utility model belongs to relevant cell culture technique field, and in particular to a kind of steril cell culture bottle.
Background technique
Cell Culture Cells culture technique is also cell clone technology, and the regular noun in biology is cell culture skill
Art.Though for entire biotechnology, or for one of them biological cloning technology, cell culture be all one must
Indispensable process, the extensive clone of cell culture inherently cell.Cell culture technology can be passed through by a cell
Mass propgation becomes many cells that are simple unicellular or seldom breaking up, this is the essential link of clone technology, Er Qiexi
The clone of born of the same parents' culture inherently cell.A large amount of cell or its metabolite are obtained by cell culture.Because of biological product
It is all to be got from cell, it is possible to say that cell culture technology is most crucial, most basic technology in biotechnology.Cell culture
The environment for focusing on asepsis pollution-free, avoid during the cultivation process cell be contaminated, but existing culture bottle single culture cell
Negligible amounts, and will use mass propgation bottle in mass propgation cell, can occupy larger space, inconvenience storage and place.
Utility model content
It is mentioned above in the background art to solve the purpose of this utility model is to provide a kind of steril cell culture bottle
Existing Tissue Culture Flask single culture cell quantity is less, and when mass propgation cell will use mass propgation bottle, inconvenience storage
And placement, and cell culture need to provide asepsis pollution-free environmental problem.
To achieve the above object, the utility model provides the following technical solutions: a kind of steril cell culture bottle, including bottle
Body, the upper surface of the bottle body are fixedly installed with convex body, and the bottom of the bottle body is equipped with groove corresponding with convex body, the bottle body
Surface be equipped with protective layer, the side of the bottle body is fixedly installed with inlet tube, and the inlet tube is set in one end far from bottle body
There is connection parent, the other side of the bottle body is fixedly installed with outlet tube, is fixedly installed with liquid-controlling valve above the outlet tube
, the surface immediately ahead of the bottle body is fixedly installed with gas-guide tube, and the gas-guide tube is fixedly connected in one end far from bottle body
Miillpore filter device, the surface of the bottle body are fixedly installed with probe tube, and the probe tube is equipped with sealing in one end far from bottle body
Lid, the surface of the bottle body inner wall is fixedly installed with cell culture vector.
Preferably, the number of the convex body is 2, is symmetrically mounted on the surface of bottle body, and the height of convex body and groove
It is highly identical.
Preferably, the quantity of the protective layer is 8, is located at the corner of bottle body, and protective layer uses silica gel material
Material.
Preferably, the inlet tube is located at the top of bottle body side, and outlet tube is located at the bottom end of the bottle body other side.
Preferably, the quantity of the probe tube is 2, is symmetrically and fixedly mounted to the two sides of gas-guide tube, and gas-guide tube is located at
The middle part on surface immediately ahead of bottle body.
Preferably, the quantity of the cell culture vector is several, is respectively symmetrically fixedly mounted on bottle body two sides inner wall
Surface, and the size of cell culture vector becomes smaller from top to bottom inside bottle body.
Compared with prior art, the utility model provides a kind of steril cell culture bottle, have it is following the utility model has the advantages that
1, the utility model is symmetrically arranged with convex body in the upper surface of bottle body, and the bottom of bottle body body is equipped with corresponding with convex body
Each culture bottle is packed together by groove in the case where Tissue Culture Flask is more by way of the clamping of convex block and groove,
Culture bottle occupied area is saved, facilitates storage and placement, convenient for safeguarding and management, while filtering equipped with micropore in one end of gas-guide tube
Diaphragm guarantees that bacterium does not enter inside bottle body in the case where guarantee provides enough air for cell culture, one end of inlet tube
Equipped with connection parent, to bottle body inside add culture solution when need to be attached with parent is connect, avoid bringing bacterium in bottle body into
Portion provides a gnotobasis for cell culture, cell is avoided to be contaminated during the cultivation process.
2, the utility model is symmetrically arranged with several cell culture vectors on the surface of bottle body inner wall, passes through cell culture
Carrier can carry out multi-layer cellular culture inside bottle body, can dramatically increase single cell culture quantity, reduce the use of culture bottle,
Convenient for unified management, while being all equipped with protective layer in each corner of bottle body, by protective layer can weaken bottle body when dropping by
The impact force arrived, avoids bottle body from breaking into pieces.
Detailed description of the invention
Attached drawing is used to provide a further understanding of the present invention, and constitutes part of specification, practical with this
Novel embodiment is used to explain the utility model together, does not constitute limitations of the present invention,
In the accompanying drawings:
Fig. 1 be the utility model proposes a kind of steril cell culture bottle structural schematic diagram；
Fig. 2 be the utility model proposes a kind of steril cell culture bottle top view；
Fig. 3 be the utility model proposes a kind of steril cell culture bottle bottle body schematic diagram of internal structure；
In figure: 1, bottle body；2, convex body；3, groove；4, protective layer；5, inlet tube；6, outlet tube；7, draining valve；8, it leads
Tracheae；9, miillpore filter device；10, probe tube；11, sealing cover；12, parent is connected；13, cell culture vector.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.Based on the embodiments of the present invention, those of ordinary skill in the art are without making creative work
Every other embodiment obtained, fall within the protection scope of the utility model.
In the description of the present invention, it should be noted that term " on ", "lower", "inner", "outside" " front end ", " after
The orientation or positional relationship of the instructions such as end ", " both ends ", " one end ", " other end " is that orientation based on the figure or position are closed
System, is merely for convenience of describing the present invention and simplifying the description, rather than the device or element of indication or suggestion meaning are necessary
It with specific orientation, is constructed and operated in a specific orientation, therefore should not be understood as limiting the present invention.In addition,
Term " first ", " second " are used for descriptive purposes only and cannot be understood as indicating or suggesting relative importance.
In the description of the present invention, it should be noted that unless otherwise clearly defined and limited, term " is pacified
Dress ", " being provided with ", " connection " etc., shall be understood in a broad sense, such as " connection ", may be a fixed connection, be also possible to detachably connect
It connects or integral type connects；It can be mechanical connection, be also possible to be electrically connected；It can be directly connected, intermediate matchmaker can also be passed through
Jie is indirectly connected, and can be the connection inside two elements.It for the ordinary skill in the art, can be with concrete condition
Understand the concrete meaning of above-mentioned term in the present invention.
Fig. 1-3 is please referred to, the utility model provides a kind of technical solution: a kind of steril cell culture bottle, including bottle body 1,
The upper surface of the bottle body 1 is fixedly installed with convex body 2, and the bottom of the bottle body 1 is equipped with groove 3 corresponding with convex body 2, by convex
Each culture bottle can be packed together by body 2 and the snap fit of groove 3, save occupied space, and the surface of the bottle body 1 is equipped with anti-
Sheath 4 can weaken the impact force suffered during dropping of bottle body 1, the fixed peace in the side of the bottle body 1 by protective layer 4
Equipped with inlet tube 5, the inlet tube 5 is equipped with connection parent 12 in one end far from bottle body 1, connects with parent 12 connect, can be by
Culture solution imports bottle body 1 by inlet tube 5, and the other side of the bottle body 1 is fixedly installed with outlet tube 6, can be incited somebody to action by outlet tube 6
Culture solution discharge inside bottle body 1, the top of the outlet tube 6 are fixedly installed with draining valve 7, and rotation draining valve 7 is controllable
The surface of the discharge of culture solution processed, 1 front of bottle body is fixedly installed with gas-guide tube 8, and the gas-guide tube 8 is far from bottle body 1
One end be fixedly connected with miillpore filter device 9, pass through gas-guide tube 8 guarantee 1 inside cell culture of bottle body needed for air, micropore filter
Diaphragm 9 can prevent bacterium and pollutant in air from entering inside bottle body 1, and the surface of the bottle body 1 is fixedly installed with probe tube
10, the probe tube 10 is equipped with sealing cover 11 in one end far from bottle body 1, and sampling equipment is inserted into probe tube 10, can be to bottle
Cell in body 1 is sampled, and the surface of 1 inner wall of bottle body is fixedly installed with cell culture vector 13, and cell is attached to carefully
It is cultivated on born of the same parents' culture carrier 13.
In the utility model, it is preferred that the number of the convex body 2 is 2, is symmetrically mounted on the surface of bottle body 1, and convex body
2 height is identical as the height of groove 3, and the guarantee culture bottle identical as 3 height of groove of convex body 2 is clamped more firm when stacking
Gu the quantity of the protective layer 4 is 8, it is located at the corner of bottle body 1, and protective layer 4 uses silica gel material, silica gel material
Expect it is cheap, elasticity preferably, the inlet tube 5 is located at the top of 1 side of bottle body, and outlet tube 6 is located at 1 other side of bottle body
Bottom end, culture solution pass through the bottom that inlet tube 5 flow to bottle body 1 from the top of bottle body 1 under gravity, and outlet tube 6 is located at
The bottom of bottle body 1 facilitates discharge culture solution, and the quantity of the probe tube 10 is 2, is symmetrically and fixedly mounted to the two of gas-guide tube 8
The probe tube 10 of side, different location can be sampled the cell culture vector 13 of 1 inside two sides of bottle body, and gas-guide tube 8 is located at
The quantity at the middle part on 1 front surface of bottle body, the cell culture vector 13 is several, is respectively symmetrically fixedly mounted on bottle body
The surface of 1 two sides inner wall, and the size of cell culture vector 13 becomes smaller from top to bottom inside bottle body 1, the bottom of bottle body 1 is equipped with
Groove 3,13 size of cell culture vector need to accordingly become smaller.
The working principle and process for using of the utility model: it after the utility model installs, is trained by several cells
It supports carrier 13 and forms multi-layer cellular culture layer in the inside of bottle body 1, significantly improve the cell culture quantity of Tissue Culture Flask, increase
The utilization rate of Tissue Culture Flask, cell are attached on cell culture vector 13 and are cultivated, with connect parent 12 connect, avoid by
Bacterium and pollutant are brought into inside bottle body 1, are imported culture solution inside bottle body 1 by inlet tube 5, under gravity, training
Nutrient solution flow to bottom from the top of bottle body 1, air needed for guaranteeing cell culture by gas-guide tube 8, the micropore of 8 one end of gas-guide tube
Filter membrane device 9 can prevent the bacterium in air from entering inside bottle body 1, and sterile environment is provided for cell culture, avoid cell by
Pollution can be sampled in sampling equipment insertion probe tube 10 cell of different location, carry out using mass propgation bottle
When cell culture, by the grafting of convex body 2 and groove 3, each Tissue Culture Flask can be packed together, save space, conveniently deposit
Storage and placement Tissue Culture Flask, when bottle body 1 is dropped, can be weakened suffered by bottle body 1 convenient for managing and maintaining by protective layer 4
Impact force, protect bottle body 1.
While there has been shown and described that the embodiments of the present invention, for the ordinary skill in the art,
It is understood that these embodiments can be carried out with a variety of variations in the case where not departing from the principles of the present invention and spirit, repaired
Change, replacement and variant, the scope of the utility model is defined by the appended claims and the equivalents thereof.
Claims (6)
1. a kind of steril cell culture bottle, including bottle body (1), it is characterised in that: the upper surface of the bottle body (1) is fixedly installed with
Convex body (2), the bottom of the bottle body (1) are equipped with groove (3) corresponding with convex body (2), and the surface of the bottle body (1) is equipped with protection
Layer (4), the side of the bottle body (1) are fixedly installed with inlet tube (5), and the inlet tube (5) sets in one end far from bottle body (1)
Have connection parent (12), the other side of the bottle body (1) is fixedly installed with outlet tube (6), and the top of the outlet tube (6) is fixed
It is equipped with draining valve (7), the surface immediately ahead of the bottle body (1) is fixedly installed with gas-guide tube (8), and the gas-guide tube (8) exists
One end far from bottle body (1) is fixedly connected with miillpore filter device (9), and the surface of the bottle body (1) is fixedly installed with probe tube
(10), the probe tube (10) is equipped with sealing cover (11) in one end far from bottle body (1), and the surface of bottle body (1) inner wall is solid
Dingan County is equipped with cell culture vector (13).
2. a kind of steril cell culture bottle according to claim 1, it is characterised in that: the number of the convex body (2) is 2
It is a, it is symmetrically mounted on the surface of bottle body (1), and the height of convex body (2) is identical as the height of groove (3).
3. a kind of steril cell culture bottle according to claim 1, it is characterised in that: the quantity of the protective layer (4) is 8
It is a, it is located at the corner of bottle body (1), and protective layer (4) uses silica gel material.
4. a kind of steril cell culture bottle according to claim 1, it is characterised in that: the inlet tube (5) is located at bottle body
(1) top of side, and outlet tube (6) is located at the bottom end of bottle body (1) other side.
5. a kind of steril cell culture bottle according to claim 1, it is characterised in that: the quantity of the probe tube (10) is
2, the two sides of gas-guide tube (8) are symmetrically and fixedly mounted to, and gas-guide tube (8) is located at the middle part on surface immediately ahead of bottle body (1).
6. a kind of steril cell culture bottle according to claim 1, it is characterised in that: the cell culture vector (13)
Quantity is several, is respectively symmetrically fixedly mounted on the surface of bottle body (1) two sides inner wall, and the size of cell culture vector (13)
Become smaller from top to bottom inside from bottle body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920057499.8U CN209508287U (en) | 2019-01-14 | 2019-01-14 | A kind of steril cell culture bottle |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920057499.8U CN209508287U (en) | 2019-01-14 | 2019-01-14 | A kind of steril cell culture bottle |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209508287U true CN209508287U (en) | 2019-10-18 |
Family
ID=68200669
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920057499.8U Expired - Fee Related CN209508287U (en) | 2019-01-14 | 2019-01-14 | A kind of steril cell culture bottle |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209508287U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
-
2019
- 2019-01-14 CN CN201920057499.8U patent/CN209508287U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112522104A (en) * | 2020-12-29 | 2021-03-19 | 弘康信达(江苏)医学研究有限公司 | Mesenchymal stem cell culture container and culture method |
| CN112522104B (en) * | 2020-12-29 | 2024-06-11 | 麦迪森(江苏)医学研究有限公司 | A mesenchymal stem cell culture container and culture method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191018 Termination date: 20210114 |