CN206089707U - Ginseng cell culture device - Google Patents
Ginseng cell culture device Download PDFInfo
- Publication number
- CN206089707U CN206089707U CN201621044065.7U CN201621044065U CN206089707U CN 206089707 U CN206089707 U CN 206089707U CN 201621044065 U CN201621044065 U CN 201621044065U CN 206089707 U CN206089707 U CN 206089707U
- Authority
- CN
- China
- Prior art keywords
- bottle
- culture
- cell
- culture medium
- ginseng
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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Abstract
The utility model discloses a ginseng cell culture device belongs to the cell culture technique field. This culture plate includes: the blake bottle, bottleneck department is equipped with bacterium filtration membrane and bottle lid, is equipped with gas pocket and blowdown pipe on the bottle lid, is equipped with netted hand -basket in the blake bottle, and netted hand -basket hangs in the blake bottle through the dead lever, and the bottle lid is stretched out to the upper end of dead lever, and the periphery of dead lever is equipped with the screw, and the dead lever passes through nut movable mounting on the bottle lid, and the well upper portion of blake bottle is equipped with the inlet, and the lower part is equipped with the liquid outlet, still is equipped with cycle wriggling agitating unit in the blake bottle, cycle wriggling agitating unit and the connection of air pressure machine. The utility model discloses a culture plate can be placed the cell of cultivateing can carry out the change of culture medium in netted hand -basket, need not subside, quick, safety, convenience. Wriggling agitating unit can guarantee that the growth conditions in the culture medium is even cycle, reduced the shearing force, avoided the cell impaired, protected the cell better.
Description
Technical field
This utility model is related to technical field of cell culture, and in particular to a kind of ginseng-cell incubator.
Background technology
Radix Ginseng is precious Chinese crude drug, and ginsenoside is the material base of Radix Ginseng physiologically active, and prior art can pass through
In vitro culture Radix Ginseng cell obtains ginsenoside.Ginseng-cell cultivation cycle is long, needs to regularly replace culture medium in incubation.
Carry out the replacing of culture medium after needing to settle cell when culture medium is changed in prior art again, the sedimentation time is generally two
My god, this process operation is bothered, and easily causes ginseng-cell damage.
Therefore design it is a kind of can easily and fast, the ginseng-cell incubator that safely changes culture medium there is very big reality
With value.
Utility model content
This utility model provides a kind of ginseng-cell incubator, can solve the problems referred to above of the prior art.
This utility model provides a kind of ginseng-cell incubator, including：Culture bottle, the bottle mouth position of the culture bottle are provided with
Bacteriological filter film and bottle cap, the bacteriological filter film are covered on bottleneck, and bottle cap is movably arranged on bottleneck, are set on the bottle cap
There are pore and blowdown pipe, the blowdown pipe is inserted in culture bottle from bottle cap, in the culture bottle, be provided with netted hand basket, it is described
Netted hand basket is suspended in culture bottle by fix bar, and bottle cap is stretched out in the upper end of the fix bar, and the periphery of fix bar is provided with spiral shell
Stricture of vagina, fix bar are movably arranged on bottle cap by nut, and the middle and upper part of the culture bottle is provided with inlet, and bottom is provided with out liquid
Mouthful, the inlet is connected with culture medium case by conduit, and the liquid outlet is connected with collecting box by conduit, on the conduit
Valve is provided with, periodic peristaltic agitating device, the periodic peristaltic agitating device and air press in the culture bottle, is additionally provided with
Connection.
It is preferred that the periodic peristaltic agitating device is straight tube-like or tubular ring.
It is preferred that sample tap is additionally provided with the culture bottle, the sample tap is connected with sample detection means.
Compared with prior art, the beneficial effects of the utility model are：Incubator of the present utility model will can be cultivated
Cell carries out the replacing of culture medium by being placed in netted hand basket, without being settled, quickly, conveniently, safely.Periodic peristaltic
Agitating device can ensure that the growth conditionss in culture medium are uniform, reduce shearing force, it is to avoid histiocyte is damaged, and preferably protects
Histiocyte.
Description of the drawings
A kind of structural representation of ginseng-cell incubator that Fig. 1 is provided for this utility model.
The structural representation of the periodic peristaltic agitating device that Fig. 2 is provided for this utility model.
Description of reference numerals：
1. culture bottle, 1-1. inlets, 1-2. liquid outlets, 2. bottle cap, 2-1. pores, 2-2. blowdown pipes, 3. netted hand basket,
4. fix bar, 5. nut, 6. culture medium case, 7. collecting box, 8. periodic peristaltic agitating device, 8-1. elastomeric materials, 8-2. cultures
Base, 8-3. gases, 8-4. gas motions direction, the point that 8-5. elastomeric materials are contacted with culture medium, 8-6. normal force sides
To.
Specific embodiment
Below in conjunction with the accompanying drawings 1, a specific embodiment of the present utility model is described in detail, it is to be understood that this
The protection domain of utility model is not limited by specific embodiment.Based on the embodiment in this utility model, this area is general
The every other embodiment obtained on the premise of creative work is not made by logical technical staff, belongs to this utility model
The scope of protection.
A kind of ginseng-cell incubator that this utility model is provided, including：Culture bottle 1, the bottle mouth position of the culture bottle 1 set
There are bacteriological filter film and bottle cap 2, the bacteriological filter film is covered on bottleneck, and bottle cap 2 is movably arranged on bottleneck, the bottle
Lid 2 is provided with pore 2-1 and blowdown pipe 2-2, and the blowdown pipe 2-2 is inserted in culture bottle 1 from bottle cap 2, the culture bottle 1
Netted hand basket 3 is provided with inside, the netted hand basket 3 is suspended in culture bottle 1 by fix bar 4, and the upper end of the fix bar 4 is stretched out
Bottle cap 2, the periphery of fix bar 4 are provided with screw thread, and fix bar 4 is movably arranged on bottle cap 2 by nut 5, in the culture bottle 1
Top is provided with inlet 1-1, and bottom is provided with liquid outlet 1-2, and the inlet 1-1 is connected with culture medium 8-2 case 6 by conduit,
The liquid outlet 1-2 is connected with collecting box 7 by conduit, and the conduit is provided with valve, is additionally provided with the cycle in the culture bottle 1
Wriggling agitating device 8, the periodic peristaltic agitating device 8 and air press connection.
During using ginseng-cell incubator of the present utility model, first explant is put in netted hand basket 3, is then injected into
Culture medium 8-2, control reaction condition carry out cell culture, using blowdown pipe 2-2 to the addition oxygen of culture bottle 1, carbon dioxide, second
The gases such as alkene.When culture medium 8-2 is changed, netted hand basket 3 can be lifted off into culture medium 8-2 as desired by fix bar 4,
Nut 5 is located at the top of bottle cap 2, after the height change of fix bar 4, its position is realized fixing with nut 5.From liquid outlet 1-2
Former culture medium 8-2 can be detached into culture bottle 1 using the pump housing, be collected with collecting box 7.Fresh culture is injected from inlet 1-1
Base 8-2.Whole process is quick, convenient, safety, time saving and energy saving.The lower end of fix bar 4 is provided with several poles, these poles
The upper end connection of one end and netted hand basket 3, so netted hand basket 3 are more stable during pull-up or decline.Netted hand basket 3
Arrange also so that the taking-up of later stage histiocyte etc. is more time saving and energy saving, and does not result in damage.Netted hand basket 3 is by acidproof resistance to
The material of alkali is made.Inlet 1-1, liquid outlet 1-2 and blowdown pipe 2-2 etc. are provided with filter, prevent bacterium infection etc..It is described
Conduit is provided with valve, when needing to extract out or add culture medium 8-2, opens valve, and the conduit at liquid outlet 1-2 can be with
Connected with collecting box 7 by the pump housing, quickly extract culture medium 8-2 out.
In addition, the periodic peristaltic agitating device 8 arranged in culture bottle 1 includes elastomeric material 8-1 (such as acid and alkali-resistances, nontoxic
Silicon rubber latex), the material of elastomeric material 8-1 expansion or shrinkage is can control inside device (such as gas 8-3, by air press
The air with certain pressure is provided for which), device is separated by elastomeric material 8-1 with culture medium 8-2, be can control inside device
The material of elastomeric material expansion or shrinkage can be along the axial direction periodic movement of elastomeric material, then elastomeric material is periodically swollen
Swollen or contraction, when elastomeric material expands, the point 8-5 contacted with elastomeric material will be moved to culture medium 8-2 direction, be produced and fortune
The vertical normal force 8-6 in dynamic direction acts on culture medium 8-2, and culture medium 8-2 will be transported toward normal force identical direction
It is dynamic, and when elastomeric material shrinks, then the motion of culture medium 8-2 is in opposite direction.Thus, through hole control elastomeric material is periodic
Expansion and contraction, culture medium 8-2 will alternately be wriggled under periodic normal force 8-6.Avoid the mechanical agitation of transmission
Shearing force destruction, enhance the heat and mass transfer in culture medium 8-2, be conducive to the growth of cell.
Further, the periodic peristaltic agitating device 8 is straight tube-like or tubular ring, if straight tube-like, can be arranged
Quantity can be two or more, be uniformly arranged in culture medium 8-2, if tubular ring, could be arranged to one, and level is put
It is placed in culture medium 8-2.
Further, sample tap is additionally provided with the culture bottle 1, the sample tap is connected with sample detection means.Pass through
Periodically or continuously sampling detection cell growth condition, takes corresponding measure, it is ensured that cell well grows in time.
Incubator of the present utility model can carry out culture medium more by the cell cultivated is placed in netted hand basket
Change, without being settled, quickly, conveniently, safely.Periodic peristaltic agitating device can ensure that the growth conditionss in culture medium are equal
It is even, reduce shearing force, it is to avoid cell damage, preferably protect cell.
Claims (3)
1. a kind of ginseng-cell incubator, including：Culture bottle (1), the bottle mouth position of the culture bottle (1) be provided with bacteriological filter film and
Bottle cap (2), the bacteriological filter film are covered on bottleneck, and bottle cap (2) is movably arranged on bottleneck, and the bottle cap (2) is provided with
Pore (2-1) and blowdown pipe (2-2), the blowdown pipe (2-2) are inserted in culture bottle (1) from from bottle cap (2), and its feature exists
In, netted hand basket (3) is provided with the culture bottle (1), the netted hand basket (3) is suspended on culture bottle (1) by fix bar (4)
Interior, bottle cap (2) is stretched out in the upper end of the fix bar (4), and the periphery of fix bar (4) is provided with screw thread, and fix bar (4) is by nut
(5) it is movably arranged on bottle cap (2), the middle and upper part of the culture bottle (1) is provided with inlet (1-1), and bottom is provided with liquid outlet (1-
2), the inlet (1-1) is connected with culture medium case (6) by conduit, and the liquid outlet (1-2) is by conduit and collecting box
(7) connect, the conduit is provided with valve, the culture bottle (1) and is additionally provided with periodic peristaltic agitating device (8), the cycle
Wriggling agitating device (8) and air press connection.
2. ginseng-cell incubator as claimed in claim 1, it is characterised in that the periodic peristaltic agitating device (8) is straight
Tubulose or tubular ring.
3. ginseng-cell incubator as claimed in claim 1, it is characterised in that be additionally provided with sample tap on the culture bottle (1),
The sample tap is connected with sample detection means.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621044065.7U CN206089707U (en) | 2016-09-08 | 2016-09-08 | Ginseng cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621044065.7U CN206089707U (en) | 2016-09-08 | 2016-09-08 | Ginseng cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206089707U true CN206089707U (en) | 2017-04-12 |
Family
ID=58476225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621044065.7U Expired - Fee Related CN206089707U (en) | 2016-09-08 | 2016-09-08 | Ginseng cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206089707U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401960A (en) * | 2017-08-16 | 2019-03-01 | 苏州米迪生物技术有限公司 | The high efficiency cell culture bottle of built-in disturbing flow device |
-
2016
- 2016-09-08 CN CN201621044065.7U patent/CN206089707U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109401960A (en) * | 2017-08-16 | 2019-03-01 | 苏州米迪生物技术有限公司 | The high efficiency cell culture bottle of built-in disturbing flow device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170412 Termination date: 20180908 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |