CN209947207U - Large-space-scale field portable micro-universe experimental device - Google Patents
Large-space-scale field portable micro-universe experimental device Download PDFInfo
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- CN209947207U CN209947207U CN201822035927.5U CN201822035927U CN209947207U CN 209947207 U CN209947207 U CN 209947207U CN 201822035927 U CN201822035927 U CN 201822035927U CN 209947207 U CN209947207 U CN 209947207U
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Abstract
The utility model provides an open-air portable microcosm experimental apparatus of big spatial dimension, including incubator, sealed lid and mount, the incubator includes flexible pipy pars contractilis and rather than the base of lower extreme sealing thread connection, is provided with the cultivation chamber in the base, and the top of pars contractilis is uncovered and is equipped with sealed lid, and sealed lid includes gas pocket and electrode hole, and gas pocket department is provided with the plug of gas pocket, and electrode hole department is provided with the plug of electrode hole, and the incubator passes through the vertical setting of mount. The device of the utility model is suitable for field microcosm experiments on large spatial scale, and can be used for observing and researching biological community structure succession, primary productivity change and the like; the device is simple to manufacture, low in price, small in occupied space, convenient to carry and sample, capable of conducting long-term culture and suitable for requirements of most scientific research teams on field micro-universe experiments.
Description
Technical Field
The utility model belongs to ecology and environmental science field, concretely relates to open-air portable micro universe experimental apparatus of big spatial dimension and method.
Background
In recent years, the influence of biodiversity on the stability of ecosystems has received increasing attention in the field of ecology. Some studies have shown that increased species diversity increases ecosystem stability, and some have shown that increased biodiversity decreases ecosystem stability. However, researchers have made a very strong debate from interpreting the results of these experiments, as there are two mechanisms that may lead to this result, one being niche complementation and the other being "sampling effect". Starting in the 90 s of the last century, scientists performed a series of experiments to examine the relationship between ecosystem function and biodiversity and attempted to reveal the mechanism of action of biodiversity on ecosystem function. Because of high controllability of the microcosm experiment, the microcosm experiment method is adopted in many researches on the relationship between the stability of an ecosystem and the biodiversity and the reliability of the ecosystem. Moreover, since the life cycle of microorganisms such as bacteria and algae is relatively short, the long-term effects of detecting species diversity in the microcosm system become feasible.
The spatial pattern of biodiversity is a central topic of ecology, however, the mechanisms driving these patterns are still unclear. Climatic factors, particularly temperature, are considered to be the primary driver of the diversity gradient on a broad spatial scale, and temperature variations may affect the abundance of species, as temperature is associated with primary productivity, limiting the range of species distribution and driving the rate of species formation. Elevated temperatures may be beneficial for greater species abundance, but may also result in the extinction of specific species in cooler areas, such as at high altitudes and latitudes. Human effects, such as increased concentrations of nutrient salts, have been identified as one of the major drivers of loss of biodiversity in recent decades. Higher temperatures and nutrient enrichment will increase the primary productivity of the ecosystem, which may further impact the abundance of species. In recent years, the influence of climate change and human activities on biodiversity is mainly used for research on plants and animals, and the research on microorganisms is less, so that the experiment of the field portable microcosm with large spatial scale still has a great vacancy, and a simple, portable and practical device is urgently needed to develop the field microcosm experiment.
SUMMERY OF THE UTILITY MODEL
The utility model discloses to a great deal of inconvenience that present big spatial dimension field portable microcosm experiment exists in field operation process, propose new portable experimental apparatus and experimental method, be used for carrying out the field microcosm experiment on the big spatial dimension. The device simple manufacture, the low price, shared space is little, portable and sample, can do long-term culture, applicable in most groups to the requirement of microorganism field culture.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a large-space-scale field portable micro universe experiment device comprises an incubator, a sealing cover, a fixing frame and a micropore sealing film, wherein the incubator comprises a telescopic part and a base, the telescopic part is in a telescopic pipe shape, the lower end of the telescopic part is in threaded sealing connection with the base, a culture cavity is arranged in the base, the top of the telescopic part is open, the sealing cover is matched with the opening,
the sealing cover comprises an air hole and an electrode hole, an air hole plug is arranged at the air hole, an electrode hole plug is arranged at the electrode hole,
the incubator is vertically arranged through the fixing frame.
Furthermore, the incubator is made of transparent materials.
Further, the telescopic part is in a corrugated pipe shape.
Further, the lateral wall middle part of pars contractilis still is provided with first thief hole, first thief hole is blockked up by first stopple and is sealed. The first plug is preferably made of transparent material.
Further, the base lateral wall still is provided with the second thief hole, the second thief hole is blockked up by the second stopple and is sealed. The second plug is preferably made of transparent material.
Further, the number of the electrode holes is 4.
Furthermore, the incubator is provided with a plurality of incubators, the fixing frame is a porous frame, and each incubator is vertically placed in one hole.
Furthermore, the fixing frame is a frame assembled by a hollow pipe and a connector. Is convenient to disassemble, assemble and carry.
Furthermore, the aperture of the micropore sealing membrane is 0.22 micron.
The utility model can adopt the general method known by the technicians in the field, adjust the specific using method according to the specific needs of the experiment, and can also adopt the following methods for use:
(1) the incubator is extended to the maximum through the telescopic part, then the incubator is vertically arranged in a field experiment area through a fixing frame, an experiment sample and water are placed into the incubator for cultivation, and the incubator is sealed through a micropore sealing film;
(2) after the culture reaches the middle stage, opening an electrode hole plug, inserting a test electrode into the electrode hole, removing a micropore sealing film, sealing the incubator by using a sealing cover, opening the gas hole plug, compressing the telescopic part to enable the electrode to contact the liquid level in the incubator, closing the gas hole plug, and recording data displayed by the electrode; aspirating a sample through the first plug; aspirating a sample through the second plug;
(3) removing the sealing cover, extending the telescopic part to the maximum, and sealing the incubator by using a microporous sealing film;
(4) after continuing culturing for a period of time, repeating or not repeating the steps (2) and (3) according to the experiment requirements;
(5) after the culture is finished, the telescopic part is separated from the base, and the sample remained in the base is taken out.
For the sealing cover provided with 4 electrode holes, a pH electrode, a temperature electrode, a dissolved oxygen electrode and a redox electrode can also penetrate through the electrode holes for detection.
The beneficial effects of the utility model reside in that:
the device of the utility model is suitable for field microcosm experiments on large spatial scale, and can be used for observing and researching biological community structure succession, primary productivity change and the like; the device is simple to manufacture, low in price, small in occupied space, convenient to carry and sample, capable of conducting long-term culture and suitable for requirements of most scientific research teams on field micro-universe experiments. For non-commercial applications, similar experimental devices may be fabricated or retrofitted in accordance with the teachings of the present invention.
Drawings
Fig. 1 is a schematic view of the overlooking structure of the large-space-scale field portable microcosm experimental device of the utility model.
Fig. 2 is the side view structure diagram of the large space scale field portable microcosm experimental device of the utility model.
FIG. 3 is a schematic view showing a state in which the incubator is compressed.
FIG. 4 is a schematic view showing the elongated state of the incubator.
Fig. 5 is a schematic view of the sealing plug.
FIG. 6 is a graph showing the gradient of the nutrient salt concentration obtained in example 1.
FIG. 7 is a graph of pH versus nutrient salt concentration gradient obtained in example 1.
In the figure, 1 is an incubator, 2 is a fixed frame, 3 is a sealing cover, 4 is an expansion part, 5 is a second hole plug, 6 is a base, 7 is a first hole plug, 8 is an air hole, and 9 is an electrode hole.
Detailed Description
As shown in figures 1-5, the large-space-scale field portable microcosm experimental device comprises an incubator, a sealing cover and a fixing frame, wherein the incubator comprises a telescopic part and a base, the telescopic part is in a telescopic pipe shape, the lower end of the telescopic part is in threaded sealing connection with the base, a culture cavity is arranged in the base, the top of the telescopic part is open, the sealing cover is adapted to the open,
the sealing cover comprises an air hole and an electrode hole, an air hole plug is arranged at the air hole, an electrode hole plug is arranged at the electrode hole,
the incubator is vertically arranged through the fixing frame.
The culture device is made of transparent materials.
The telescopic part is in a corrugated pipe shape.
The lateral wall middle part of pars contractilis still is provided with first thief hole, first thief hole is blockked up by first stopple and is sealed. The first hole plug is made of transparent materials.
The base lateral wall still is provided with the second thief hole, the second thief hole is blockked up by the second stopple and is sealed. The second hole plug is made of transparent materials.
The number of the electrode holes is 4.
The incubator is provided with a plurality of incubators, the fixing frame is a porous frame, and each incubator is vertically placed in one hole.
The fixing frame is a frame assembled by a hollow pipe and a connector. Is convenient to disassemble, assemble and carry.
The experimental device also comprises a micropore sealing film.
The aperture of the micropore sealing membrane is 0.22 micron.
Example 1
The device is adopted to carry out large-scale field micro-universe experiments, and comprises the following steps:
(1) after reaching the sampling point, building a fixed frame;
(2) the culture device is stretched to the maximum degree through the telescopic part and then is sequentially placed into the fixing frame.
(3) Taking off the sealing cover, and putting experimental articles such as water, sediments and the like into a culture device;
(4) a membrane with a pore size of 0.22 μm was placed on the open seal of the incubator.
(5) After the middle period of culture, the electrode hole plug is opened, the electrodes such as the universal electrode, the pH electrode, the temperature electrode and the like are inserted into the electrode hole, and the sealing cover is put back on the incubator.
(6) And (3) opening the air hole plug, shrinking the incubator downwards until each electrode can contact the liquid level in the incubator, closing the air hole plug, and recording data displayed by each electrode.
(7) The sealing cap and the electrode were removed, the incubator was stretched to the maximum extent, and if the water level in the incubator was too low, water was added to the incubator to continue the incubation, and the membrane with a pore size of 0.22 μm was sealed.
(8) And (3) using an injector, inserting the needle head into the first hole plug, extracting a water sample, filling the water sample into the sampling bottle, and covering the sampling bottle.
(9) And (4) when the culture is continued for a period of time, collecting the sample by adopting the method of the step (8).
(10) And (5) repeating the steps (5) and (6).
(11) And (4) collecting the sample by adopting the method in the step (8).
(12) After the cultivation, will the pars contractilis with the base separation, carefully pour out the water that persists in the base, then scrape the deposit of bottom with the spoon in the sampling bottle to cover the sampling bottle.
(13) The base of the incubator is placed back on the incubator, the incubator is compressed downwards to the minimum volume, the sealing cover is covered, and the incubator is collected.
(14) The fixing frame is disassembled into a hollow tube and a tee joint and is put into a packaging bag.
(15) And (6) counting the instrument accessories and finishing the experiment.
To illustrate the implementation of the device, the Taihu lake sediment is used for carrying out field microcosm experiments by using temperature changes caused by the elevation gradient of the south edge of the Qinghai-Tibet plateau, so as to determine the influence of temperature and nutrient salt concentration on the microbial community structure and biodiversity. Above-mentioned field experiment is because the concentration gradient and the control group of designing nutritive salt, so a set of sample need the incubator 30 at least, and the quantity and the volume of required incubator are all bigger for field experiment, and it is also relatively more troublesome to fix in the field at the incubator moreover, and the operation when taking a sample is also comparatively loaded down with trivial details, in addition, owing to need utilize the height above sea level gradient, so at the in-process that sets up the sample. The portability and the weight of the experimental equipment are also relatively high. The utility model discloses to above-mentioned difficult point, the design is simple portable, and portable's experimental apparatus provides one set of complete use scheme. With respect to the study and experimental design of the above experiments, we provide the following solutions and implementation methods, detailed below:
3 sampling points are arranged in the altitude range of 800-3600 meters in the Qinghai-Tibet plateau, and 30 cultivators, 40 hollow pipes, nutrient salts and sediment are prepared for each point. Firstly, quickly installing 40 hollow pipes into a fixing frame capable of placing 30 culture cavities, then stretching the culture devices to the maximum height, and placing the culture devices into the fixing frame, wherein each culture device is placed into a grid. And opening a sealing cover of the incubator, and adding in-situ water into the incubator, wherein the liquid level is 2cm below the highest position of the incubator. And then sequentially adding the Taihu lake sediment which is sterilized in advance and nutrient salt with concentration gradient into the culture device, wherein 10 concentration gradients are counted from 0 to 10, and because the experiment does not need a closed space and needs to be communicated with the outside, a layer of 0.22 micron membrane is arranged on the bottle mouth, so that outside bacteria can not enter. All 10 spots were set and cultured in situ for 30 days. In the middle-term detection, the membrane is taken down, then the sealing cover is covered, the air hole plug is opened, the incubator is contracted downwards until the sealing cover is in contact with the liquid level, then the air hole plug is covered, the electrode hole plug is opened, and the electrodes such as pH and temperature are inserted for detecting middle-term data. After the culture, the process of sampling is carried out, the sample is firstly placed at the place where each sample is placed, 20ml of water sample is taken from the upper part and is filled into a 20ml sampling bottle, then a needle cylinder is inserted into a first hole plug and a second hole plug, 20ml of water is pumped out from the incubator and is filled into the sampling bottle, then each incubator is carefully taken out, the taking-out process is carried out without shaking as much as possible, then the culture base is unscrewed from the lower part, after the residual water is poured out, bottom sediments are scraped into the 20ml sampling bottle by a small spoon, and the sampling can be completed. After sampling was completed, the incubator was compressed to a minimum height and the fixture system was disassembled into 40 hollow tubes for removal. Finally, the garbage can not be left in the field.
The results of our experiment are shown in fig. 6 and fig. 7, there is a clear green gradient from low concentration to high concentration of nutrient salt, and as the concentration of nutrient salt increases, the pH also gradually increases, which indicates that the increase of nutrient salt leads to the increase of biological community and further leads to the increase of photosynthesis, and the results are in line with the expected experimental conclusion.
Claims (10)
1. A large-space-scale field portable micro universe experiment device is characterized by comprising an incubator, a sealing cover, a fixing frame and a micropore sealing film, wherein the incubator comprises a telescopic part and a base, the telescopic part is in a telescopic pipe shape, the lower end of the telescopic part is in threaded sealing connection with the base, a culture cavity is arranged in the base, the top of the telescopic part is open, the sealing cover is matched with the open,
the sealing cover comprises an air hole and an electrode hole, an air hole plug is arranged at the air hole, an electrode hole plug is arranged at the electrode hole,
the incubator is vertically arranged through the fixing frame.
2. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein the incubator is transparent.
3. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein a first sampling hole is further disposed in the middle of the side wall of the expansion portion, and the first sampling hole is blocked and sealed by a first hole plug.
4. The large-spatial-scale field portable microcosm experimental apparatus according to claim 1, wherein the telescoping portion is bellows-shaped.
5. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein the base sidewall is further provided with a second sampling hole, and the second sampling hole is sealed by a second plug.
6. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein there are 4 electrode apertures.
7. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein the plurality of incubators are provided, the holder is a multi-hole frame, and each incubator is vertically placed in one hole.
8. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein the fixing frame is a frame assembled by hollow pipes and connectors.
9. The large-space-scale field portable microcosm experimental apparatus according to claim 1, wherein the pore size of the microporous sealing membrane is 0.22 μm.
10. The large-spatial-scale field portable microcosm experimental apparatus according to claim 1, wherein the telescoping portion is bellows-shaped.
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| CN201822035927.5U CN209947207U (en) | 2018-12-06 | 2018-12-06 | Large-space-scale field portable micro-universe experimental device |
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| CN201822035927.5U CN209947207U (en) | 2018-12-06 | 2018-12-06 | Large-space-scale field portable micro-universe experimental device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109410704A (en) * | 2018-12-06 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | Large spatial scale field portable microcosm experiment device and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109410704A (en) * | 2018-12-06 | 2019-03-01 | 中国科学院南京地理与湖泊研究所 | Large spatial scale field portable microcosm experiment device and method |
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