CN115612615A

CN115612615A - Culture dish convenient for observation for biological cell culture

Info

Publication number: CN115612615A
Application number: CN202211045158.1A
Authority: CN
Inventors: 任语
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-08-30
Filing date: 2022-08-30
Publication date: 2023-01-17

Abstract

The application discloses be applied to culture dish field's a biological cell is cultivated with culture dish convenient to survey, this culture dish is through the setting of devices such as board that turns over, turn over the board under the initial condition and be in the horizontality, when cultivateing biological cell, place the inner wall on top at the bottom of the dish with the cell, then make the inboard of card at the bottom of the dish lid and putting into the mounting groove cultivate at the bottom of the dish, during the cultivation, if the culture state of cell at the bottom of the dish needs observation, then can the starter motor drive the lead screw rotatory and make rack rebound at the effect of connecting block, the effect drive through gear and bull stick turns over the board rotatory to the vertical state after the rack rebound, at this moment the staff will turn over the board and open the growth state of cell at the bottom of the dish with regard to permeable inner door observation, compare with traditional culture dish and incubator, the convenience of observing time has been improved, the inside of having avoided the incubator simultaneously exposes and causes the pollution to it in the air, stability when having improved cell culture.

Description

Culture dish convenient for observation for biological cell culture

Technical Field

The application relates to the field of culture dishes, in particular to a culture dish convenient for observation for biological cell cultivation.

Background

The culture dish is a laboratory vessel used for culturing microorganisms or cells, consists of a plane disc-shaped bottom and a cover, is generally made of glass or plastics, and is a box body device with controllable temperature and used for culturing microorganisms, plants and animal cells, and is widely applied to tests such as constant-temperature culture, constant-temperature reaction and the like.

The culture dish generally will be put and cultivate biological cell in the incubator, and the incubator generally is sealed environment, if want to observe biological cell at the growth state of different stages, then need open the incubator and take out the culture dish and observe, and the incubator is when opening, and miscellaneous fungus in the air can get into the inside of incubator and cause its pollution, simultaneously, if the culture dish quantity of placing is relatively more, need take out the culture dish one by one and observe and put back, the operation is inconvenient.

Disclosure of Invention

The utility model aims to solve the problem that a culture dish is inconvenient to observe in an incubator, compared with the prior art, the utility model provides a culture dish convenient to observe for biological cell cultivation, which comprises an incubator and a dish cover, the inner wall of the dish cover is clamped with a dish bottom, the outer wall of one side of the incubator is hinged with an outer door and an inner door, the inner door is positioned at the inner side of the outer door, the inner side of the incubator is provided with a plurality of uniformly distributed turning plates, the top surface of the turning plate is penetrated with a plurality of uniformly distributed laying grooves, the dish cover is positioned at the inner side of the laying grooves, a baffle is fixedly connected between the inner walls of the top end and the bottom end of the incubator, a rotating rod is rotatably connected between the side wall of one side of the baffle and the inner wall of the incubator, a rack is slidably connected between the side wall of the other side of the baffle and the inner wall of the incubator, the outer wall of the rotating rod is fixedly connected with one end of the turning plate, one end of the rotating rod penetrates through the baffle and is fixedly connected with a gear, the gear and the gear are meshed with the rack, the side wall of the rack is fixedly connected with a connecting block, a lead screw is rotatably connected between the inner walls of the top end of the incubator, a lead screw is penetrated through the connecting block and is connected with a motor, the top end of the lead screw is fixedly connected with the lead screw; the realization is compared with traditional culture dish and incubator, has improved the convenience of observing time measuring, has avoided the inside of incubator to expose in the air and to cause the pollution to it simultaneously, stability when having improved cell culture.

Optionally, the outer wall of the opening of the dish cover is fixedly connected with a middle ring, the outer wall of the bottom of the dish is fixedly connected with a top ring, the turning plate is fixedly connected with a bottom ring close to the outer wall of the placing groove, and the dish cover can be better connected with the placing groove through the arrangement.

Optionally, the inside fixedly connected with magnetic ring of zhonghuan, the equal fixedly connected with iron ring in inside of apical ring and bottom ring, the effect of magnetic ring accessible iron ring is with the position of adsorbing at the mounting groove at the bottom of ware lid and the ware, has avoided turning over the board and has broken away from the mounting groove at the bottom of ware lid and the ware when the upset.

Optionally, the inner door includes a carrier plate and a glass plate, and a plurality of observation grooves corresponding to the mounting grooves are formed through a side wall of the carrier plate.

Optionally, the lateral wall fixedly connected with lens membrane of support plate near the observation groove, the inside of observation groove is full of the pure water.

Optionally, the lens membrane is made for high transparent elastic material, and the lens membrane is protruding form, through above-mentioned setting, when observing the cell, can play the effect of enlargeing to the cell wall of cultivateing on the dish bottom, can more subtle observation cultivate the change of cell, the further convenience when having increased the observation.

Optionally, a through hole is formed between every two adjacent observation grooves, the side wall, far away from the carrier plate, of the glass plate is fixedly connected with an L rod, and the L rod is communicated with one of the observation grooves.

Optionally, the inner wall fixedly connected with of incubator bottom supplies to press pipe and electric telescopic link, and the expansion end of electric telescopic link extends to the inboard and fixedly connected with piston that supply to press the pipe, piston and the inner wall sliding connection who supplies to press the pipe.

It is optional, the bottom of L pole and supply to press to manage and keep away from between the one end of electric telescopic link flexible siphunculus, the inside of pressing the pipe is full of the pure water for the confession, through the aforesaid setting, if need change the magnification of lens membrane, then can start electric telescopic link and promote the piston and remove right, the pure water that supplies to press in the pipe this moment makes the lens membrane more protruding by the inside of extrusion to the observation tank, the protruding back focal length of lens membrane becomes long, the magnification increases, can be more clear observe the cell of cultivateing at the bottom of the ware.

Optionally, the lateral wall fixedly connected with control panel of incubator, control panel and electric telescopic link and motor electric connection, through the setting of control panel, the running state of control electric telescopic link and motor that can be better.

Compare in prior art, the advantage of this application lies in:

(1) This culture dish is through the setting of devices such as board that turns over, turn over the board under the initial condition and be in the horizontality, when cultivateing biological cells, place the inner wall on top at the bottom of the dish with the cell, then make the inboard of card at the bottom of the dish lid and putting into the mounting groove cultivate, during the cultivation, if the cultivation state of cell in the bottom of the dish needs to be observed, then can the starter motor drive the lead screw rotatory and make rack rebound at the effect of connecting block, the effect drive through gear and bull stick behind the rack rebound turns over board rotatory to the vertical state, at this moment the staff will turn over the board and open the growth state of cell at the bottom of just permeable inner door observation dish, compare with traditional culture dish and incubator, the convenience of observing time measuring has been improved, the inside of having avoided the incubator simultaneously exposes and causes the pollution to it in the air, stability when having improved cell culture.

(2) The outer wall of the opening of the dish cover is fixedly connected with a middle ring, the outer wall of the bottom of the dish is fixedly connected with a top ring, the outer wall of the turning plate close to the placing groove is fixedly connected with a bottom ring, and through the arrangement, the dish cover can be better connected with the placing groove.

(3) The lens membrane is made for high transparent elastic material, and the lens membrane is protruding form, through above-mentioned setting, when observing the cell, can play the effect of enlargeing to the cell wall of cultivateing on the dish bottom, can more subtle observation cultivate the change of cell, the further convenience when having increased the observation.

(4) The bottom of L pole and supply to press to manage and keep away from between the one end of electric telescopic link flexible siphunculus, the inside of pressing the pipe is full of the pure water to the supply, through the aforesaid setting, if the magnification of lens membrane needs to be changed, then can start electric telescopic link and promote the piston and remove right, supplies the pure water in the pressing pipe this moment to be extruded to the inside messenger lens membrane of observation tank more protruding, the protruding back focus of lens membrane is elongated, the magnification increases, the cell of cultivateing at the bottom of the dish of observation that can be more clear.

(5) The lateral wall fixedly connected with control panel of incubator, control panel and electric telescopic link and motor electric connection, through the setting of control panel, the running state of control electric telescopic link and motor that can be better.

Drawings

FIG. 1 is a state diagram of the present application with an open outer door;

FIG. 2 is a state diagram of the present application with the outer and inner doors open;

FIG. 3 is a front perspective view of the present application;

FIG. 4 is a schematic view of the present application with the flap in a vertical position;

FIG. 5 is a top perspective sectional view of an incubator according to the present application;

FIG. 6 is a side perspective sectional view of the incubator of the present application;

FIG. 7 is a perspective sectional view of the present application at the flap;

FIG. 8 is a perspective cross-sectional view of the capsule and the bottom of the capsule of the present application;

FIG. 9 is an exploded perspective view of the present application at the inner door;

fig. 10 is a front sectional view of a carrier according to the present application.

The reference numbers in the figures illustrate:

1. an incubator; 101. a partition plate; 2. an outer door; 3. an inner door; 301. a carrier plate; 302. an observation tank; 303. a glass plate; 304. a lens film; 305. a through hole; 4. turning over a plate; 401. a rotating rod; 402. a bottom ring; 403. a placing groove; 5. a gear; 501. a rack; 502. connecting blocks; 6. a screw rod; 7. a motor; 8. a dish cover; 801. a middle ring; 802. a magnetic coil; 9. a dish bottom; 901. a top ring; 10. a pressure supply pipe; 11. an electric telescopic rod; 12. an L-bar; 13. a flexible through pipe; 14. and (6) a control screen.

Detailed Description

The technical solutions in the embodiments of the present application will be described below clearly and completely with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments, and all other embodiments obtained by a person of ordinary skill in the art without making creative efforts based on the embodiments in the present application belong to the protection scope of the present application.

Example 1:

the application discloses a culture dish convenient for observation for biological cell cultivation, please refer to fig. 1-8, which comprises an incubator 1 and a dish cover 8, wherein a dish bottom 9 is clamped on the inner wall of the dish cover 8, and the culture dish is characterized in that the outer wall of one side of the incubator 1 is hinged with an outer door 2 and an inner door 3, the inner door 3 is positioned on the inner side of the outer door 2, a plurality of uniformly distributed turning plates 4 are arranged on the inner side of the incubator 1, a plurality of uniformly distributed placing grooves 403 are formed in the top surface of the turning plates 4 in a penetrating manner, the dish cover 8 is positioned on the inner side of the placing grooves 403, a partition plate 101 is fixedly connected between the inner walls of the top end and the bottom end of the incubator 1, a rotating rod 401 is rotatably connected between the side wall of one side of the partition plate 101 and the inner wall of the incubator 1, a rack 501 is slidably connected between the side wall of the other side of the partition plate 101 and the inner wall of the incubator 1, the outer wall of the rotating rod 401 is fixedly connected with one end of the turning plate 4, one end of the rotating rod 401 penetrates through the partition plate 101 and is fixedly connected with a gear 5, the gear 5 is meshed with the rack 501, the side wall of the rack 501 is fixedly connected with a connecting block 502, the side wall of the rack 501 is fixedly connected with a screw rod 6 connected with the inner wall of the connecting block 502, the connecting block 6 of the side of the incubator 1, the motor 502 is fixedly connected with the top end of a screw rod 6, and a motor 7 of the motor 502, and a screw rod 502 of the motor 502 is fixedly connected with the motor 502, and a screw rod 502, and a motor 7 of the motor, and a screw rod 502 is fixedly connected with the motor, and a screw rod 502; turning over board 4 under initial condition is in the horizontality, place the cell when cultivateing biological cell at the bottom of the dish the inner wall on 9 tops then make 9 cards at the bottom of the dish the inner wall of ware lid 8 and put into the inboard of mounting groove 403 and cultivate, during the cultivation, if need observe the culture state of cell at the bottom of the dish at the bottom of 9, then can starting motor 7 drive lead screw 6 rotatory and make rack 501 rebound at the effect of connecting block 502, rack 501 rebound back drives through gear 5 and bull stick 401 effect and turns over board 4 rotatory to the vertical state, at this moment the staff will turn over board 4 and open the growth state of cell at the bottom of the dish with regard to the permeable inner door 3 observation ware, compare with traditional culture dish and incubator, the convenience of observation time measuring has been improved, the inside of having avoided incubator 1 exposes in the air and causes the pollution to it simultaneously, stability when having improved the cell culture.

Referring to fig. 7-8, the outer wall of the opening of the dish cover 8 is fixedly connected with a middle ring 801, the outer wall of the dish bottom 9 is fixedly connected with a top ring 901, the turning plate 4 is close to the outer wall of the placing groove 403 and is fixedly connected with a bottom ring 402, through the arrangement, the dish cover 8 can be better connected to the placing groove 403, the inner portion of the middle ring 801 is fixedly connected with a magnetic ring 802, the inner portions of the top ring 901 and the bottom ring 402 are fixedly connected with iron rings, when the dish cover 8 and the dish bottom 9 are placed inside the placing groove 403, the magnetic ring 802 can adsorb the dish cover 8 and the dish bottom 9 to the placing groove 403 under the action of the iron rings, and the turning plate 4 is prevented from being separated from the placing groove 403 when the dish cover 8 and the dish bottom 9 are turned over.

Referring to fig. 1-5 and 9-10, the inner door 3 includes a carrier plate 301 and a glass plate 303, wherein a plurality of observation slots 302 corresponding to the placement slots 403 are formed through the side wall of the carrier plate 301; specifically, turn over board 4 when rotatory to the vertical state, observation groove 302 and mounting groove 403 one-to-one and observation groove 302 are located the positive side of mounting groove 403, support plate 301 is close to the lateral wall fixedly connected with lens membrane 304 of observation groove 302, the inside of observation groove 302 is full of the pure water, lens membrane 304 is made for high transparent elastic material, lens membrane 304 is protruding form, through the aforesaid setting, after turning over board 4 and rotatory to the vertical state, because lens membrane 304 is protruding state, when observing the cell, can play the effect of enlargeing to the cell wall of cultivateing on the dish bottom 9, can more subtle observation cultivate the change of cell, further increased the convenience when observing.

Referring to fig. 2-5 and 9-10, a through hole 305 is formed between every two adjacent observation grooves 302, the side wall of the glass plate 303 far from the support plate 301 is fixedly connected with an L rod 12, the L rod 12 is communicated with one of the observation grooves 302, the inner wall of the bottom end of the incubator 1 is fixedly connected with a pressure supply pipe 10 and an electric telescopic rod 11, the movable end of the electric telescopic rod 11 extends to the inner side of the pressure supply pipe 10 and is fixedly connected with a piston, the piston is slidably connected with the inner wall of the pressure supply pipe 10, a flexible through pipe 13 is communicated between the bottom end of the L rod 12 and one end of the pressure supply pipe 10 far from the electric telescopic rod 11, the interior of the pressure supply pipe 10 is filled with purified water, if the magnification of the lens film 304 needs to be changed, the electric telescopic rod 11 can be started to push the piston to move rightwards, the purified water in the pressure supply pipe 10 is squeezed into the observation grooves 302 to make the lens film 304 more convex, the focal length is increased after the lens film 304 is convex, and the magnification is increased, and cells cultured on the dish bottom 9 can be observed more clearly.

Referring to fig. 2, a control panel 14 is fixedly connected to a side wall of the incubator 1, the control panel 14 is electrically connected to the electric telescopic rod 11 and the motor 7, and the operation states of the electric telescopic rod 11 and the motor 7 can be better controlled by setting the control panel 14.

The above description is only for the preferred embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art should be considered to be within the scope of the present application, and all equivalent substitutions or changes according to the technical solutions and modifications of the present application should be covered by the scope of the present application.

Claims

1. The utility model provides a culture dish convenient to observe for biological cell is cultivated, includes incubator (1) and ware lid (8), the inner wall joint of ware lid (8) has the ware end (9), its characterized in that, the outer wall of incubator (1) one side articulates there are outer door (2) and inner door (3), inner door (3) are located the inboard of outer door (2), the inboard of incubator (1) is provided with a plurality of evenly distributed turns over board (4), the top surface that turns over board (4) runs through and sets up a plurality of evenly distributed mounting groove (403), ware lid (8) are located the inboard of mounting groove (403), fixedly connected with baffle (101) between the inner wall of incubator (1) top and bottom, rotate between the lateral wall of baffle (101) one side and the inside wall of incubator (1) and be connected with bull stick (401), sliding connection has rack (501) between the lateral wall of baffle (101) opposite side and the inside wall of incubator (1), the outer wall and the one end fixed connection of turning over board (4) of bull stick (401) are connected with rack (501) through gear (5), the one end fixed connection piece (501) and gear (5) and be connected with rack (501) and gear (5), the utility model discloses a cultivation box, including incubator (1), lead screw (6) through connection piece (502) and threaded connection with it, the top fixedly connected with motor (7) of incubator (1), the output of motor (7) and the top fixed connection of lead screw (6).

2. The culture dish convenient for observation in biological cell culture according to claim 1, wherein the outer wall of the opening of the dish cover (8) is fixedly connected with a middle ring (801), the outer wall of the dish bottom (9) is fixedly connected with a top ring (901), and the outer wall of the turning plate (4) close to the placing groove (403) is fixedly connected with a bottom ring (402).

3. A vessel for observing biological cells as claimed in claim 2, wherein said middle ring (801) has a magnetic ring (802) fixedly connected to the inside thereof, and said top ring (901) and said bottom ring (402) have iron rings fixedly connected to the inside thereof.

4. A vessel for observing a biological cell culture according to claim 1, wherein said inner door (3) comprises a carrier plate (301) and a glass plate (303), and a plurality of observation slots (302) corresponding to the receiving slots (403) are formed through the side wall of said carrier plate (301).

5. A vessel as claimed in claim 4, wherein the support plate (301) is fixed with a lens membrane (304) near the side wall of the observation slot (302), and the observation slot (302) is filled with purified water.

6. A vessel for observing biological cells as claimed in claim 5, wherein said lens membrane (304) is made of a highly transparent elastic material, and said lens membrane (304) is convex.

7. A culture dish for cultivating biological cells and facilitating observation according to claim 6, wherein a through hole (305) is formed between every two adjacent observation grooves (302), an L-shaped rod (12) is fixedly connected to the side wall of the glass plate (303) far away from the carrier plate (301), and the L-shaped rod (12) is communicated with one of the observation grooves (302).

8. A vessel for cultivating biological cells, which is convenient for observation according to claim 7, wherein the inner wall of the bottom end of the incubator (1) is fixedly connected with a pressure supply tube (10) and an electric telescopic rod (11), the movable end of the electric telescopic rod (11) extends to the inner side of the pressure supply tube (10) and is fixedly connected with a piston, and the piston is slidably connected with the inner wall of the pressure supply tube (10).

9. A vessel for observing biological cells as claimed in claim 8, wherein a flexible tube (13) is connected between the bottom end of the L-shaped rod (12) and the end of the pressure supply tube (10) far away from the electric telescopic rod (11), and the pressure supply tube (10) is filled with purified water.

10. The culture dish convenient for observation in biological cell culture is characterized in that a control screen (14) is fixedly connected to the side wall of the culture box (1), and the control screen (14) is electrically connected with the electric telescopic rod (11) and the motor (7).