CN218710505U - A kind of shaker cultivation device for liquid fermentation of cicadae strain - Google Patents
A kind of shaker cultivation device for liquid fermentation of cicadae strain Download PDFInfo
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Abstract
Description
技术领域technical field
本实用新型涉及蝉花培养技术领域,具体为一种蝉花菌株液体发酵用摇床培养装置。The utility model relates to the technical field of cicadae cultivation, in particular to a shaking table cultivation device for liquid fermentation of cicadae strains.
背景技术Background technique
野外采集的蝉花标本可采用常规分离方法获得野生菌株,通过初筛和复筛两步筛选出菌丝生长速度快、生物量和目标活性物质产量高的优良菌株作为生产用菌株,根据生产和科研的需求,采用临时性保存(斜面菌种4℃保存以及蒸馏水室温保持)和永久性保存(液氮保存)的方法保存菌种,蝉花液体发酵和固体发酵各具利弊,生产上采取二者结合的双相发酵工艺,通过液体发酵生产菌丝体作为固体发酵的种子,而通过固体发酵来生产孢梗束或分生孢子等不同产品,或获得某些活性代谢物质。Cicada specimens collected in the field can be obtained by conventional isolation methods to obtain wild strains, and the excellent strains with fast mycelial growth rate, high biomass and high yield of target active substances are selected as production strains through the two steps of primary screening and secondary screening. For the needs of scientific research, the methods of temporary preservation (slant strains at 4°C and distilled water at room temperature) and permanent preservation (liquid nitrogen preservation) are used to preserve the strains. Liquid fermentation and solid fermentation of cicadae have their own advantages and disadvantages. Two methods are adopted in production. The combination of the two-phase fermentation process, the production of mycelia through liquid fermentation as the seeds of solid fermentation, and the production of different products such as spore bundles or conidia through solid fermentation, or to obtain certain active metabolites.
在蝉花培养的过程中,需要制备孢子悬浮液,即将母种块接种在瓶中的液体培养基中,然后将其放置在恒温摇床中培养得到菌悬液,再将液体进行发酵,把菌悬液按比例混合在培养基中放置在摇床中进行发酵,随着研究工作的深入,蝉花培养生产工艺不断得到改进和优良,但是由于蝉花喜光,现有的摇床培养装置主要为单面或双面的箱体结构,同时也不方便培养基的观察,且一次菌悬液的培养时间通常为6-7天,过程中若需要观察需要将培养瓶取出,导致污染问题,影响蝉花菌株液体的发酵,针对现有技术的不足,本实用新型提供了一种蝉花菌株液体发酵用摇床培养装置。In the process of cultivating cicadae, it is necessary to prepare spore suspension, that is, to inoculate the mother seed block in the liquid medium in the bottle, then place it in a constant temperature shaker to cultivate the bacterial suspension, and then ferment the liquid, and put The bacterial suspension is mixed in proportion in the culture medium and placed in a shaker for fermentation. With the deepening of research work, the production technology of cicadae cultivation has been continuously improved and excellent. However, due to the joy of cicadae, the existing shaker cultivation It is a single-sided or double-sided box structure, and it is not convenient to observe the culture medium, and the culture time of a bacterial suspension is usually 6-7 days. If observation is required during the process, the culture bottle needs to be taken out, resulting in pollution problems. Aiming at the deficiencies in the prior art, the utility model provides a shaker cultivation device for the liquid fermentation of the cicadae strain.
实用新型内容Utility model content
针对现有技术的不足,本实用新型提供了一种蝉花菌株液体发酵用摇床培养装置,解决了上述背景技术中提出的问题。Aiming at the deficiencies of the prior art, the utility model provides a shaker culture device for the liquid fermentation of the cicadae strain, which solves the problems raised in the above-mentioned background technology.
为实现以上目的,本实用新型通过以下技术方案予以实现:一种蝉花菌株液体发酵用摇床培养装置,包括箱体,所述箱体 的内部安装有转盘装置,所述转盘装置上设置有瓶架,所述箱体的顶部配置有温度湿度控制器,所述箱体的外部安装有挡板。In order to achieve the above object, the utility model is realized through the following technical solutions: a shaker culture device for the liquid fermentation of cicadae strains, comprising a casing, the inside of the casing is equipped with a turntable device, and the turntable device is provided with A bottle rack, a temperature and humidity controller is arranged on the top of the box, and a baffle is installed on the outside of the box.
可选的,所述箱体上设置有箱门,且箱门与箱体的接触面上连接有密封胶条,以及箱体内部的顶部活动连接有可自由转动的限定快,并且箱体的四周设置有透明板结构。Optionally, the box body is provided with a box door, and a sealing strip is connected to the contact surface between the box door and the box body, and the top inside the box body is movably connected with a freely rotatable limiter, and the box body A transparent board structure is arranged around.
可选的,所述转盘装置安装在箱体内部的底部,且转盘装置上设置有与瓶架对应的长条卡槽,以及转盘装置受箱体的控制。Optionally, the turntable device is installed at the bottom inside the box, and the turntable device is provided with a long slot corresponding to the bottle rack, and the turntable device is controlled by the box.
可选的,所述瓶架活动插接在转盘装置上,且瓶架的底部连接有插板,以及瓶架的上半部分连接有固定板,所述瓶架的中心轴上活动套接有固定架,固定架上开设有与瓶架底部凹槽一一对应的圆孔。Optionally, the bottle holder is movably plugged on the turntable device, and the bottom of the bottle holder is connected with a plugboard, and the upper part of the bottle holder is connected with a fixing plate, and the central axis of the bottle holder is movably socketed with a The fixing frame is provided with circular holes corresponding to the grooves at the bottom of the bottle holder.
可选的,所述挡板活动连接在箱体的外部,且挡板可转动。Optionally, the baffle is movably connected to the outside of the box, and the baffle can be rotated.
可选的,所述温度湿度控制器安装在箱体的顶部,且温度湿度控制器受箱体的控制。Optionally, the temperature and humidity controller is installed on the top of the box, and the temperature and humidity controller is controlled by the box.
本实用新型提供了一种蝉花菌株液体发酵用摇床培养装置,具备以下有益效果:The utility model provides a shaker culture device for liquid fermentation of cicadae strains, which has the following beneficial effects:
1、该蝉花菌株液体发酵用摇床培养装置,通过将瓶架顶部与底部与转盘设置为活动插接结构,能够方便瓶架的取出,也方便了瓶架的清理,同时也能够提高培养瓶的放置效率,同时通过将箱体四周设置为透明结构,能够方便培养的观察以及为蝉花菌株的生长提供对应的光照,提高培养效率。1. The shaker cultivation device for the liquid fermentation of the cicadae strain can facilitate the removal of the bottle holder and the cleaning of the bottle holder by setting the top and bottom of the bottle holder and the turntable as a movable plug-in structure, and can also improve the cultivation efficiency. At the same time, by setting the surroundings of the box as a transparent structure, it can facilitate the observation of cultivation and provide corresponding light for the growth of cicadae strains, improving the cultivation efficiency.
2、该蝉花菌株液体发酵用摇床培养装置,通过在瓶架的顶部与底部设置插块,能够实现瓶架在转盘上的活动连接,从而方便瓶架的取出,提高培养瓶的放置效率,通过将固定板活动套接在瓶架上,能够在放置好培养瓶后通过将固定板套接在培养瓶外部实现培养瓶的相对固定,从而使得该瓶架能够适应不同规格大小的培养瓶,提高该培养装置的适配性。2. The shaker culture device for the liquid fermentation of the cicadae strain can realize the flexible connection of the bottle rack on the turntable by setting inserts on the top and bottom of the bottle rack, thereby facilitating the removal of the bottle rack and improving the placement efficiency of the culture bottle , by connecting the fixed plate to the bottle rack, the relative fixation of the culture bottle can be achieved by connecting the fixed plate to the outside of the culture bottle after the culture bottle is placed, so that the bottle rack can adapt to culture bottles of different sizes , to improve the adaptability of the culture device.
3、该蝉花菌株液体发酵用摇床培养装置,通过将该培养装置的四周设置透明板,能够方便摇床培养装置工作过程中对培养瓶的观察,同时也能够为蝉花菌株的培养发酵提供对应的光照,达到了对发酵菌株进行实时查看的效果,从而能够在蝉花菌株发酵过程中对蝉花菌株的异常发酵及时作出反应,提高了蝉花菌株发酵的效率。3. The shaker culture device for the liquid fermentation of the cicadae strain liquid, by setting transparent plates around the culture device, can facilitate the observation of the culture bottle in the working process of the shaker culture device, and can also be used for the cultivation and fermentation of the cicadae strain. The corresponding light is provided to achieve the effect of real-time viewing of the fermentation strains, so that the abnormal fermentation of the cicadae strains can be reacted in time during the fermentation process of the cicadae strains, and the efficiency of the fermentation of the cicadae strains is improved.
4、总之,该蝉花菌株液体发酵用摇床培养装置,通过设置方便取出的瓶架,能够提高培养瓶的放置效率,方便瓶架的清理,通过设置活动式固定架,能够使该瓶架适应不同规格的培养瓶,提高培养瓶的固定效果以及稳定性,并且通过将箱体四周设置为透光结构,能够方便培养瓶培养过程中的观察,同时也能够为蝉花菌株的发酵提供对应的光照,提高该摇床培养装置对蝉花菌株发酵的培养效率。4. In a word, the shaker culture device for the liquid fermentation of the cicadae strain can improve the placement efficiency of the culture bottle and facilitate the cleaning of the bottle rack by setting a bottle rack that is convenient to take out. By setting a movable fixed frame, the bottle rack can be Adapt to culture bottles of different specifications, improve the fixing effect and stability of the culture bottle, and set the surrounding of the box as a light-transmitting structure, which can facilitate the observation of the culture bottle during the culture process, and can also provide corresponding support for the fermentation of the cicadae strain. The illumination can improve the culture efficiency of the shaker culture device for the fermentation of cicadae strains.
附图说明Description of drawings
图1为本实用新型结构示意图;Fig. 1 is the structural representation of the utility model;
图2为本实用新型的仰视结构示意图;Fig. 2 is the structural representation of looking up of the utility model;
图3为本实用新型内部的俯视结构示意图;Fig. 3 is a top view structural schematic diagram inside the utility model;
图4为本实用新型内部的仰视结构示意图;Fig. 4 is the structural schematic diagram of looking up inside the utility model;
图5为本实用新型瓶架的仰视结构示意图;Fig. 5 is the structural schematic diagram of looking up of the bottle holder of the present utility model;
图6为本实用新型瓶架的俯视结构示意图。Fig. 6 is a top view structural schematic diagram of the bottle rack of the present invention.
图中:1、箱体;2、转盘装置;3、瓶架;31、固定板;32、插板;33、固定架;4、挡板;5、温度湿度控制器。In the figure: 1. Box body; 2. Turntable device; 3. Bottle rack; 31. Fixed plate; 32. Insert plate; 33. Fixed frame; 4. Baffle plate; 5. Temperature and humidity controller.
具体实施方式Detailed ways
下面将结合本实用新型实施例中的附图,对本实用新型实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例仅仅是本实用新型一部分实施例,而不是全部的实施例。The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. example.
请参阅图2-6,本实用新型提供一种技术方案:箱体1内部的底部安装有转盘装置2,转盘装置2上设置有条状卡块,方便瓶架3的固定,转盘装置2受箱体1上控制器的控制,能够带动瓶架3进行匀速转动,转盘装置2上设置有瓶架3,瓶架3的底部连接有插板32,且瓶架3的顶部活动插接在箱体1顶部的转动机构上,使得瓶架3能够随着转盘装置2的驱动而进行转动,同时也方便瓶架3的取出,从而方便了培养瓶的放置以及该装置的清理,瓶架3的中部活动套接有固定架33,固定架33上设置有与瓶架3上位置一一对应的圆孔,且该圆孔比瓶架3上的培养瓶放置孔的内径小,用于固定培养瓶的瓶颈,放置培养瓶时,把固定架33提起,放置培养瓶完成后放下固定架33,使得固定架33上的圆孔一一套接在培养瓶瓶颈的外部,达到培养瓶固定的效果,使得瓶架3能够适配不同规格的培养瓶,提高了该装置的适配性,且瓶架3的上半部分连接有固定板31,用于人手的抓握,方便瓶架3的移动。Please refer to Figures 2-6, the utility model provides a technical solution: a
请参阅图1-4,箱体1的四周设置有透光板,能够方便菌株接受光照,同时使用者也可以对菌株进行实时观察,从而能够及时作出对应,提高蝉花菌株的培育效率,箱体1的顶部安装有温度湿度控制器5,能够对箱体1内部的温湿度进行控制,使得箱体1内部环境处于适合蝉花菌株发酵的状态,箱体1内部的顶部设置有与转盘装置2中心对应的转动块,该转动块用于瓶架3顶部的固定,能自由转动,箱体1上箱门的外部设置有挡板4,用于箱门的关闭,且箱体1上箱门的内侧连接有密封条,用于箱体1的密封。Please refer to Figure 1-4, there are light-transmitting panels around the
综上所述,该蝉花菌株液体发酵用摇床培养装置,使用时,白该装置放置在合适的位置,连接好电源,打开箱门,手握在固定板31的底部,把瓶架3从箱体1的内部取出,并将瓶架3放置在操作桌面上,把固定架33向上提拉,然后把装好培养基的培养瓶一一放置在瓶架3上,放置好后放下固定架33,使得固定架33上的圆孔一一套接在培养瓶的瓶颈上,然后手握在固定板31的底部,把瓶架3放回箱体1的内部,使得插板32插接在转盘装置2上,同时瓶架3的顶部也插接在箱体1内顶部的对应位置,关闭箱门,并把挡板4向上转动,完成箱门的锁合,通过箱体1上的操作面板进行设置,该装置启动后,转盘装置2根据设置进行匀速转动,温度湿度控制器5对箱体1内部的空气温度与湿度进行控制,使用者通过控制外部光照对培养基进行补光以及实时观察。In summary, the shaker cultivation device for the liquid fermentation of the cicadae strain is used. The device is placed in a suitable position, the power supply is connected, the door is opened, and the bottom of the
在本实用新型的描述中,需要说明的是,术语“中心”、“上”、“下”、“左”、“右”、“竖直”、“水平”、“内”、“外”等指示的方位或位置关系为基于附图所示的方位或位置关系,仅是为了便于描述本实用新型和简化描述,而不是指示或暗示所指的装置或元件必须具有特定的方位、以特定的方位构造和操作,因此不能理解为对本实用新型的限制;术语“第一”、“第二”、“第三”仅用于描述目的,而不能理解为指示或暗示相对重要性,此外,除非另有明确的规定和限定,术语“安装”、“相连”、“连接”应做广义理解,例如,可以是固定连接,也可以是可拆卸连接,或一体地连接;可以是机械连接,也可以是电连接;可以是直接相连,也可以通过中间媒介间接相连,可以是两个元件内部的连通。对于本领域的普通技术人员而言,可以具体情况理解上述术语在本实用新型中的具体含义。而且,术语“包括”、“包含”或者其任何其他变体意在涵盖非排他性的包含,从而使得包括一系列要素的过程、方法、物品或者设备不仅包括那些要素,而且还包括没有明确列出的其他要素,或者是还包括为这种过程、方法、物品或者设备所固有的要素。In the description of the present utility model, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer" The orientation or positional relationship indicated by etc. is based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, use a specific Therefore, it cannot be construed as a limitation on the present invention; the terms "first", "second", and "third" are only used for descriptive purposes, and cannot be interpreted as indicating or implying relative importance. In addition, Unless otherwise clearly specified and limited, the terms "installation", "connection" and "connection" should be interpreted in a broad sense, for example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection, It can also be an electrical connection; it can be a direct connection, or an indirect connection through an intermediary, or an internal communication between two components. Those of ordinary skill in the art can understand the specific meanings of the above terms in the present utility model in specific situations. Furthermore, the term "comprises", "comprises" or any other variation thereof is intended to cover a non-exclusive inclusion such that a process, method, article, or apparatus comprising a set of elements includes not only those elements, but also includes elements not expressly listed. other elements of or also include elements inherent in such a process, method, article, or device.
尽管已经示出和描述了本实用新型的实施例,对于本领域的普通技术人员而言,可以理解在不脱离本实用新型的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型,本实用新型的范围由所附权利要求及其等同物限定。Although the embodiments of the present invention have been shown and described, those skilled in the art can understand that various changes and modifications can be made to these embodiments without departing from the principle and spirit of the present invention , replacements and modifications, the scope of the present utility model is defined by the appended claims and their equivalents.
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| CN116396838A (en) * | 2023-06-01 | 2023-07-07 | 山东零点生物工程有限公司 | Composite microbial fermentation inoculant and extraction and culture equipment thereof |
| CN116396838B (en) * | 2023-06-01 | 2023-08-01 | 山东零点生物工程有限公司 | Composite microbial fermentation inoculant and extraction and culture equipment thereof |
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