CN221235589U

CN221235589U - Cell culture tank convenient to change culture medium

Info

Publication number: CN221235589U
Application number: CN202322868337.1U
Authority: CN
Inventors: 罗健
Original assignee: Chengdu Huisheng Biotechnology Co ltd
Current assignee: Chengdu Huisheng Biotechnology Co ltd
Priority date: 2023-10-25
Filing date: 2023-10-25
Publication date: 2024-06-28
Anticipated expiration: 2033-10-25

Abstract

The utility model provides a cell culture tank convenient for replacing a culture medium, and belongs to the technical field of cell culture. Including the cell body, the horizontal rotation is provided with the carousel on the cell body diapire, has offered a plurality of first through-holes along circumference on the carousel, and first recess has been seted up to the one end that driving motor was kept away from to first through-hole, and first recess is embedded to be equipped with the mounting panel, and the carousel bottom is equipped with first drive assembly, and the cell body top is equipped with the apron, has seted up the second through-hole on the apron, has articulated sealed lid on the apron, establishes two limiting plates in the mounting panel both sides on the carousel, and two limiting plate heights are the same to the distance of apron with the carousel, still are equipped with second drive assembly in the mounting panel both sides on the carousel. In the embodiment of the utility model, the culture medium to be replaced can be transported out of the tank body only through the second through holes, and other culture mediums in the tank body are surrounded by the corresponding limiting plates, so that the possibility that other culture mediums are polluted by bacteria in the air is reduced, and the cell culture effect of the tank body is improved.

Description

Cell culture tank convenient to change culture medium

Technical Field

The utility model relates to the technical field of cell culture, in particular to a cell culture tank convenient for changing a culture medium.

Background

The culture medium is an artificially prepared nutrient for growth and maintenance of microorganisms, plant tissues and animal tissues, and in the fields of biology, medicine and the like, cell culture is carried out by using the culture medium, a plurality of culture mediums containing cells are placed in a grid of a culture tank at intervals, and culture is carried out for a period of time in an environment arranged in the culture tank.

After the cell culture is completed, the worker needs to take out the medium in the culture tank and replace it with a new medium to perform the cell culture of the next batch. In the conventional replacement mode, a worker is required to open the cover plate of the culture tank, and then scratch the culture medium in the grid of the culture tank by means of a tool. However, this causes other media in the culture tank to be directly exposed to the outside air, and the entry of impurities such as bacteria in the air into the culture tank tends to cause failure of cell culture on the other media.

Disclosure of utility model

In view of the above, the present utility model provides a cell culture tank that facilitates replacement of a culture medium.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

The utility model provides a cell culture tank convenient to change culture medium, which comprises a tank body, the cell body top is equipped with the opening, the horizontal rotation is provided with the carousel on the cell body diapire, the cell body bottom is provided with the driving motor who is used for driving the carousel pivoted, a plurality of first through-holes have been seted up along circumference interval run-through on the carousel, the first recess that the diameter is greater than first through-hole is seted up to the one end that driving motor was kept away from to first through-hole, first recess is embedded to be equipped with the mounting panel that is used for placing the culture medium, be equipped with on the cell body bottom diapire and be used for driving the mounting panel in the first through-hole to slide along vertical direction and advance/go out the first drive assembly of cell body, the opening part at cell body top can be dismantled and be connected with transparent apron, be equipped with the second through-hole that is used for supplying the mounting panel to pass on the apron, it has the sealed lid that is used for the shutoff second through-hole to articulate on the apron, two limiting plates that are used for limiting the culture medium slope in the carousel rotation in the process along the radial symmetry sliding connection of mounting panel, two limiting plates height are the same with the distance of carousel to apron, it is used for forming and being used for depositing the airtight chamber of culture medium to enclose on the mounting panel to slide on the mounting panel through two limiting plates, still be equipped with two limiting plates on the opposite direction drive the mounting panel and drive assembly.

Further, the first driving assembly comprises a first air cylinder, the cylinder body of the first air cylinder is connected with the bottom wall in the groove body, the piston rod of the first air cylinder is vertically upwards and is opposite to the first through hole on the turntable, and a push block with the size being matched with the first through hole is arranged at one end, close to the turntable, of the piston rod of the first air cylinder.

Further, a second groove with a size matched with the pushing block is formed in one side, close to the first cylinder, of the mounting plate, and a magnetic block used for being in magnetic attraction connection with the mounting plate is embedded in one surface, used for being in contact with the mounting plate, of the pushing block.

Further, the second driving assembly comprises two second air cylinders, the two second air cylinders are respectively and correspondingly arranged on one sides of the two limiting plates, the cylinder body of each second air cylinder is connected with the rotary table, and the piston rod of each second air cylinder is connected with the corresponding side wall of the corresponding limiting plate.

Further, a sealing ring is arranged on one surface of the sealing cover, which is used for being in contact with the second through hole, along the circumferential direction, and a third groove which is matched with the sealing ring is formed in the outer side of the second through hole on the cover plate.

Further, the cover plate is provided with a limiting ring with an inner diameter matched with the groove body along the circumferential direction.

The beneficial effects of the utility model are as follows: when one of them mounting panel on the carousel rotates to directly over the first cylinder, can drive two limiting plates on the mounting panel to the direction of keeping away from the mounting panel through the second cylinder and slide, open sealed lid afterwards, first cylinder can drive the mounting panel to vertically upwards move out the second through-hole on the apron after sealed lid is opened to the staff changes, has reduced other media by the bacterial contamination's in the air possibility, has the advantage that improves cell culture effect to the cell.

Drawings

FIG. 1 is a schematic diagram showing the overall structure of a cell culture tank for facilitating medium replacement according to an embodiment of the present application.

FIG. 2 is a schematic diagram showing the internal structure of a cell culture tank for facilitating the replacement of a culture medium according to an embodiment of the present application.

FIG. 3 is a schematic cross-sectional view of a cell culture vessel for facilitating medium exchange according to an embodiment of the present application.

Fig. 4 is an enlarged partial schematic view of the portion a in fig. 3.

Wherein, 1, a groove body; 2. a turntable; 21. a first through hole; 3. a driving motor; 4. a mounting plate; 41. a second groove; 5. a first drive assembly; 51. a first cylinder; 52. a pushing block; 53. a magnetic block; 6. a cover plate; 61. a second through hole; 62. a third groove; 63. a limiting ring; 7. sealing cover; 71. a seal ring; 8. a limiting plate; 9. a second drive assembly; 91. and a second cylinder.

Detailed Description

In order to better understand the above technical solutions, the following detailed description will refer to the accompanying drawings and specific embodiments.

The embodiment of the application discloses a cell culture tank convenient for replacing a culture medium, which comprises a tank body 1, wherein an opening is formed in the top of the tank body 1, a rotary table 2 is horizontally arranged on the inner bottom wall of the tank body 1 in a rotating mode, a mounting frame is connected with the bottom of the tank body 1 through bolts, a driving motor 3 is connected with the mounting frame through bolts, and a rotating shaft of the driving motor 3 vertically penetrates into the tank body 1 and is connected with the central position of the bottom of the rotary table 2 through bolts. A plurality of first through holes 21 are formed in the rotary table 2 at intervals along the circumferential direction, a first groove with the diameter larger than that of the first through holes 21 is formed in one end, away from the driving motor 3, of the first through holes 21, a mounting plate 4 for placing a culture medium is embedded in the first groove, and a first driving assembly 5 for driving the mounting plate 4 in the first through holes 21 to slide into/out of the tank body 1 along the vertical direction is mounted on the inner bottom wall of the tank body 1 at the bottom of the rotary table 2. The opening part at the top of the groove body 1 is provided with a transparent cover plate 6, the cover plate 6 is provided with a second through hole 61 for the mounting plate 4 to pass through, and the cover plate 6 is hinged with a sealing cover 7 for sealing the second through hole 61 through a hinge. Two limiting plates 8 used for limiting the inclination of the culture medium in the rotation process of the turntable 2 are connected to the two sides of the mounting plate 4 in a sliding manner along the radial symmetry direction of the mounting plate 4 on the turntable 2, the height of the two limiting plates 8 is the same as the distance between the turntable 2 and the cover plate 6, a closed storage cavity used for storing the culture medium can be formed on the mounting plate 4 by sliding and surrounding the two limiting plates 8, and a second driving assembly 9 used for driving the two limiting plates 8 to slide oppositely is further arranged on the turntable 2 on two sides of the mounting plate 4.

In the embodiment of the application, the turntable 2 is driven to rotate by the driving motor 3, one of the mounting plates 4 on the turntable 2 can be rotated to be right above the first driving assembly 5, then the two limiting plates 8 on the mounting plate 4 can be driven to slide away from the mounting plate 4 by the second driving assembly 9, then the sealing cover 7 is opened, and the first driving assembly 5 can drive the mounting plate 4 to vertically move upwards out of the second through hole 61 on the cover plate 6 after the sealing cover 7 is opened, so that the worker can replace the sealing cover. The culture medium to be replaced can be transported out of the tank body 1 only through the second through hole 61 on the cover plate 6, so that the air amount entering the tank body 1 during sampling is reduced. Meanwhile, as the height of the limiting plate 8 is the same as the distance from the rotary table 2 to the cover plate 6, other culture mediums in the tank body 1 are always surrounded in the storage cavity by the corresponding limiting plate 8, and the risk that other culture mediums in the tank body 1 are polluted by impurity bacteria in the air is further reduced.

Referring to fig. 4, the first driving assembly 5 includes a first cylinder 51, a cylinder body of the first cylinder 51 is bolted to the inner bottom wall of the tub 1, and a piston rod of the first cylinder 51 is vertically upward and faces the first through hole 21 of the turntable 2. The piston rod of the first cylinder 51 is bolted to the end of the turntable 2 close to the push block 52, which is sized to fit the first through hole 21. When one mounting plate 4 on the turntable 2 rotates to the position right above the first air cylinder 51, the sealing cover 7 is opened, and the first air cylinder 51 can drive the pushing block 52 to vertically move upwards so that the mounting plate 4 passes through the second through hole 61 on the cover plate 6, and therefore a worker can take out the culture medium on the mounting plate 4 of the tank body 1.

In order to prevent the mounting plate 4 from sliding and deviating relative to the push block 52 during vertical sliding, a second groove 41 with a size matched with that of the push block 52 is formed in one side, close to the first air cylinder 51, of the mounting plate 4, a mounting groove is formed in one surface, in contact with the mounting plate 4, of the push block 52, and a magnetic block 53 used for being in magnetic attraction connection with the mounting plate 4 is embedded in the mounting groove. The push block 52 is inserted into the second groove 41 on the mounting plate 4, and the magnetic block 53 on the push block 52 is magnetically fixed with the mounting plate 4, so that the stability of the mounting plate 4 in the process of vertically sliding into/out of the groove body 1 can be improved.

The second driving assembly 9 comprises two second air cylinders 91, and the two second air cylinders 91 are respectively and correspondingly arranged on one sides of the two limiting plates 8. The cylinder body of the second cylinder 91 is connected with the turntable 2 through bolts, and the piston rod of the second cylinder 91 is connected with the side wall of the corresponding limiting plate 8 through bolts. When the turntable 2 rotates so that one of the mounting plates 4 faces the first cylinder 51, the second cylinders 91 on both sides of the limiting plate 8 drive the corresponding limiting plate 8 to slide away from the mounting plate 4, so that the first cylinder 51 can drive the mounting plate 4 to move vertically upwards.

One surface of the sealing cover 7, which is used for being in contact with the second through hole 61, is connected with a sealing ring 71 through bolts along the circumferential direction, the sealing ring 71 can be made of flexible materials such as rubber, and a third groove 62 matched with the sealing ring 71 is formed in the cover plate 6 outside the second through hole 61. The sealing cover 7 is rotated to enable the sealing ring 71 on the sealing cover 7 to be inserted into the third groove 62, so that the tightness of the groove body 1 in cell culture is improved.

The apron 6 has set firmly the spacing ring 63 of internal diameter and cell body 1 adaptation along circumference an organic whole, in inserting spacing ring 63 through cell body 1, the inside wall of spacing ring 63 and the lateral wall contact of cell body 1 opening part can further improve the leakproofness of cell body 1.

The implementation principle of the cell culture tank convenient for replacing the culture medium in the embodiment of the application is as follows: in a use process of a cell culture tank convenient for replacing a culture medium, a driving motor 3 drives a turntable 2 to rotate, one mounting plate 4 on the turntable 2 can be rotated to be right above a first air cylinder 51, then two limiting plates 8 on the mounting plate 4 can be driven to slide in a direction away from the mounting plate 4 through a second air cylinder 91, then a sealing cover 7 is opened, and the first air cylinder 51 can drive the mounting plate 4 to vertically move out of a second through hole 61 on a cover plate 6 after the sealing cover 7 is opened, so that a worker can take the cell culture tank. Compared with the traditional replacement mode, the culture medium to be replaced can be transported out of the tank body 1 only through the second through hole 61 on the cover plate 6, so that the air amount entering the tank body 1 during sampling is reduced. Meanwhile, as the height of the limiting plate 8 is the same as the distance from the rotary table 2 to the cover plate 6, other culture mediums in the tank body 1 are always surrounded by the corresponding limiting plate 8, the risk that other culture mediums in the tank body 1 are polluted by impurity bacteria in the air is reduced, and the cell culture device has the advantage of improving the cell culture effect of the tank body 1.

It will be apparent to those skilled in the art that while preferred embodiments of the present utility model have been described, additional variations and modifications may be made to these embodiments once the basic inventive concepts are known to those skilled in the art. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the utility model. It will be apparent to those skilled in the art that various modifications and variations can be made to the present utility model without departing from the spirit or scope of the utility model. Thus, it is intended that the present utility model also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.

Claims

1. A cell culture tank convenient for changing culture medium, characterized in that: including cell body (1), cell body (1) top is equipped with the opening, horizontal rotation is provided with carousel (2) on the diapire in cell body (1), cell body (1) bottom is provided with and is used for driving carousel (2) pivoted driving motor (3), a plurality of first through-holes (21) have been seted up along circumference interval run-through on carousel (2), first through-hole (21) are kept away from driving motor (3) one end and are seted up the first recess that the diameter is greater than first through-hole (21), first recess is embedded to be equipped with mounting panel (4) that are used for placing the culture medium, carousel (2) bottom is equipped with on cell body (1) diapire and is used for driving mounting panel (4) in first through-hole (21) to slide into/go out of cell body (1) along vertical direction, the opening part detachably at cell body (1) top is connected with transparent apron (6), be equipped with on apron (6) and be used for supplying mounting panel (4) to pass's first recess, be equipped with mounting panel (4) on the apron (6) are used for two sides (4) to slide on two radial direction symmetry mounting panel (4) and are connected with mounting panel (8), the height of the two limiting plates (8) is the same as the distance from the rotary table (2) to the cover plate (6), the two limiting plates (8) slide and encircle on the mounting plate (4) to form a closed storage cavity for storing culture mediums, and the rotary table (2) is also provided with second driving assemblies (9) for driving the two limiting plates (8) to slide oppositely on two sides of the mounting plate (4).

2. A cell culture tank for facilitating medium replacement according to claim 1, wherein: the first driving assembly (5) comprises a first air cylinder (51), a cylinder body of the first air cylinder (51) is connected with the inner bottom wall of the groove body (1), a piston rod of the first air cylinder (51) is vertically upwards and is opposite to a first through hole (21) on the rotary table (2), and a push block (52) with the size matched with the first through hole (21) is arranged at one end, close to the rotary table (2), of the piston rod of the first air cylinder (51).

3. A cell culture tank for facilitating medium replacement according to claim 2, wherein: a second groove (41) with a size matched with that of the pushing block (52) is formed in one side, close to the first cylinder (51), of the mounting plate (4), and a magnetic block (53) used for being in magnetic attraction connection with the mounting plate (4) is embedded in one surface, used for being in contact with the mounting plate (4), of the pushing block (52).

4. A cell culture tank for facilitating medium replacement according to claim 1, wherein: the second driving assembly (9) comprises two second air cylinders (91), the two second air cylinders (91) are respectively and correspondingly arranged on one sides of the two limiting plates (8), the cylinder body of each second air cylinder (91) is connected with the rotary table (2), and the piston rod of each second air cylinder (91) is connected with the side wall of the corresponding limiting plate (8).

5. A cell culture tank for facilitating medium replacement according to claim 1, wherein: one surface of the sealing cover (7) used for being in contact with the second through hole (61) is provided with a sealing ring (71) along the circumferential direction, and a third groove (62) matched with the sealing ring (71) is formed in the outer side of the second through hole (61) on the cover plate (6).

6. A cell culture tank for facilitating medium replacement according to claim 1, wherein: and the cover plate (6) is circumferentially provided with a limiting ring (63) with the inner diameter matched with the groove body (1).