CN217948152U

CN217948152U - Cell culture device

Info

Publication number: CN217948152U
Application number: CN202222032055.3U
Authority: CN
Inventors: 沈文华; 吴昊天; 王铜生
Original assignee: Nanjing Zilong Biotechnology Co ltd
Current assignee: Nanjing Zilong Biotechnology Co ltd
Priority date: 2022-08-03
Filing date: 2022-08-03
Publication date: 2022-12-02
Anticipated expiration: 2032-08-03

Abstract

The utility model discloses a cell culture device, the mounting bracket, the incubator, annotate liquid mechanism, cultivate mechanism and rotary mechanism, it includes the water pump to annotate liquid mechanism, annotate the liquid case, electric putter, the connecting pipe, annotate the liquid dish, solenoid valve and nozzle, fixed mounting has the incubator on the mounting bracket, incubator top end fixed mounting annotates the liquid case, it has the water pump to annotate liquid case right side fixed mounting, and the output and the incubator in-connection of water pump communicate, top end fixed mounting has the connecting pipe in the incubator, connecting pipe bottom fixed mounting annotates the liquid dish, the top and the notes liquid incasement intercommunication of connecting pipe, the bottom of connecting pipe and the interior intercommunication of annotating the liquid dish. This cell culture device can be according to the cell condition to single cell filling culture solution through annotating liquid mechanism, is convenient for add according to the different cell conditions to cell culture's success rate has further been improved, and can make the nozzle peg graft to corresponding culture chamber during the filling, when can avoiding filling, the culture solution splashes and causes the waste of resource.

Description

Cell culture device

Technical Field

The utility model relates to a cell culture technical field specifically is a cell culture device.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. The cell culture technology can make a single cell undergo mass culture to form a simple single cell or a multi-cell with little differentiation, which is an indispensable link of the cloning technology, and the cell culture is the cloning of the cell. Cell culture techniques are important and commonly used in cell biology research methods, and a large number of cells can be obtained through cell culture, and signal transduction, anabolism, growth and proliferation of cells and the like of the cells can be researched.

The cell culture device is needed to be used in the process of culturing cells, the common cell culture device is provided with a cell culture box, the cell culture box is an advanced instrument for culturing cells, tissues and bacteria, is key equipment necessary for developing immunology, oncology, genetics and bioengineering, and is widely applied to scientific research and production of microorganisms, agricultural science, YL experiments and the like.

The structure that is used for filling up the culture solution in current cell culture case is comparatively simple, can't be according to the needs of cultivateing filling up the culture solution in the single culture dish, consequently can't separately add according to the cell condition, leads to cell culture's success rate to be lower.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a cell culture device to solve the problem that proposes in the above-mentioned background art.

In order to achieve the above purpose, the utility model provides a following technical scheme: the utility model provides a cell culture device, includes mounting bracket, incubator, notes liquid mechanism, cultivation mechanism and rotary mechanism, it includes the water pump to annotate liquid mechanism, annotate liquid case, electric putter, connecting pipe, notes liquid dish, solenoid valve and nozzle to annotate liquid mechanism, fixed mounting has the incubator on the mounting bracket, incubator top end fixed mounting annotates the liquid case, annotate liquid case right side fixed mounting has the water pump, just the output and the incubator in-connection of water pump communicate, top end fixed mounting has the connecting pipe in the incubator, connecting pipe bottom end fixed mounting annotates the liquid dish, the top and the notes liquid incasement intercommunication of connecting pipe, the bottom and the interior intercommunication of notes liquid dish of connecting pipe, it has a plurality of nozzles to annotate liquid dish bottom end fixed mounting, and is a plurality of equal fixed mounting has the solenoid valve in the nozzle, the outside fixed mounting that lies in the connecting pipe on the notes liquid dish has a plurality of electric putter, and a plurality of the equal fixed mounting in incubator top in electric putter's top is annotated.

Optionally, the connecting pipe is arranged for a gooseneck, and the use of the device is facilitated by the telescopic characteristic of the gooseneck.

Optionally, rotary mechanism includes mount pad, roating seat, pivot, first bevel gear, second bevel gear and motor, bottom fixed mounting has the roating seat in the incubator, the roating seat internal rotation is connected with the pivot, the top fixed mounting of pivot has the mount pad, the bottom fixed mounting of pivot has first bevel gear, the right side fixed mounting that the mounting bracket bottom is located first bevel gear has the motor, fixed mounting has second bevel gear on the output of motor, second bevel gear meshing connects on first bevel gear, drives second bevel gear through motor work and rotates, and second bevel gear drives first bevel gear and rotates, and first bevel gear drives the pivot and rotates, and the pivot drives the mount pad and rotates, can make the culture solution of filling rock the distribution even, is favorable to the cultivation of cell.

Optionally, the top end of the rotating shaft extends out of the rotating seat, and the bottom end of the rotating shaft extends out of the bottom end of the mounting frame.

Optionally, cultivate the mechanism including cultivateing chamber, spacing ring, slide bar, spring, card strip, draw-in groove, cultivation dish, mounting groove and solid fixed ring, be provided with a plurality of mounting grooves on the mount pad, it is a plurality of equal sliding connection has the cultivation dish in the mounting groove, and is a plurality of the top of cultivateing the dish all is provided with cultivates the chamber, the bottom outside fixed mounting of mount pad has solid fixed ring, gu fixed ring's upper portion is provided with the spacing ring, gu fixed ring's top fixed mounting has a plurality of slide bars, and is a plurality of outside the spacing ring is all extended to the top of slide bar, it is a plurality of all the cover is equipped with the spring on the slide bar, and is a plurality of the equal fixed mounting in bottom of spring is on gu fixed ring, just the equal fixed mounting in top of spring just the inboard of spacing ring offsets with a plurality of cultivation dishes and sets up, through pulling the spacing ring downwards, cultivates the dish and loses spacingly this moment, and the cultivation dish that will take off pulls towards the outside for cultivate the dish and break away from the mounting groove, can take out the cell of cultivateing, and be convenient for the staff observes and studies.

Optionally, each equal fixed mounting in both sides of cultivateing the dish has the card strip, every the corresponding card strip department has all seted up the draw-in groove in the mounting groove, the card strip is pegged graft in the draw-in groove, and is spacing to cultivateing the dish through the card strip, is favorable to the use of this equipment.

Compared with the prior art, the beneficial effects of the utility model are that:

1. through mutually supporting of water pump, notes liquid case, electric putter, connecting pipe, notes liquid dish, solenoid valve and nozzle, can be convenient for add according to the different cell condition to single cell filling culture solution according to the cell condition to cell culture's success rate has further been improved, and can make the nozzle peg graft to the culture intracavity that corresponds when filling, when can avoiding filling, the culture solution splashes and causes the waste of resource.

2. Through cultivateing chamber, spacing ring, slide bar, spring, card strip, draw-in groove, cultivation dish, mounting groove and solid fixed ring mutually support for cultivate the dish and can with the mount pad between quick installation dismantlement, the staff of being convenient for takes out the cell observation experiment, avoided traditional cultivation in-process simultaneously need put the cell and cultivate on the diaphragm, because the diaphragm is inseparable with the laminating of container inner wall, lead to the cell to be difficult to take out, and cause the impaired problem of cell when taking out easily.

3. Through mutually supporting of mount pad, roating seat, pivot, first bevel gear, second bevel gear and motor for after filling the culture solution, can utilize the mount pad to rotate and make the culture solution rock evenly distributed in cultivateing the intracavity, be favorable to cell culture's use.

Drawings

FIG. 1 is a schematic view of the overall structure of a cell culture apparatus according to the present invention;

FIG. 2 is a rear view of a cell culture apparatus according to the present invention;

FIG. 3 is a cross-sectional view of a cell culture apparatus of the present invention;

FIG. 4 is a sectional view of the seed culture mechanism of the cell culture apparatus of the present invention.

In the figure: 1. a mounting frame; 2. an incubator; 3. a liquid injection mechanism; 301. a water pump; 302. injecting a liquid tank; 303. an electric push rod; 304. a connecting pipe; 305. a liquid injection tray; 306. an electromagnetic valve; 307. a nozzle; 4. a culture mechanism; 401. a culture chamber; 402. a limiting ring; 403. a slide bar; 404. a spring; 405. clamping the strip; 406. a card slot; 407. a culture tray; 408. mounting grooves; 409. a fixing ring; 5. a rotation mechanism; 501. a mounting seat; 502. a rotating base; 503. a rotating shaft; 504. a first bevel gear; 505. a second bevel gear; 506. an electric motor.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Please refer to fig. 1 to 4, the utility model provides a cell culture device, including mounting bracket 1, incubator 2, annotate liquid mechanism 3, culture mechanism 4 and rotary mechanism 5, annotate liquid mechanism 3 and include water pump 301, annotate liquid case 302, electric putter 303, connecting pipe 304, annotate liquid dish 305, solenoid valve 306 and nozzle 307, fixed mounting has incubator 2 on the

mounting bracket

1, 2 top fixed mounting of incubator has notes liquid case 302, annotate liquid case 302 right side fixed mounting have water pump 301, and water pump 301's output and 2 in-connection of incubator, top fixed mounting has connecting pipe 304 in incubator 2, connecting pipe 304 bottom fixed mounting has notes liquid dish 305, the top and the notes liquid case 302 in intercommunication of connecting pipe 304, the bottom and the notes liquid dish 305 in the intercommunication of connecting pipe 304, it has a plurality of nozzles 307 to annotate liquid dish 305 bottom fixed mounting, equal fixed mounting has solenoid valve 306 in a plurality of nozzles 307, it has a plurality of electric putter 303 to annotate the outside fixed mounting that lies in connecting pipe 304 on liquid dish 305, the equal fixed mounting in the top of a plurality of electric putter 303 is on incubator 2.

The connecting pipe 304 is a gooseneck, the use of the device is facilitated by utilizing the characteristic that the gooseneck can stretch, the rotating mechanism 5 comprises a mounting seat 501, a rotating seat 502, a rotating shaft 503, a first bevel gear 504, a second bevel gear 505 and a motor 506, the rotating seat 502 is fixedly mounted at the inner bottom end of the incubator 2, the rotating shaft 503 is rotatably connected with the rotating seat 502, the mounting seat 501 is fixedly mounted at the top end of the rotating shaft 503, the first bevel gear 504 is fixedly mounted at the bottom end of the rotating shaft 503, the motor 506 is fixedly mounted at the right side of the first bevel gear 504 at the bottom end of the mounting frame 1, the second bevel gear 505 is fixedly mounted at the output end of the motor 506, the second bevel gear 505 is meshed and connected with the first bevel gear 504, the second bevel gear 505 is driven to rotate by the motor 506, the first bevel gear 504 drives the rotating shaft 503 to rotate, the rotating shaft 503 drives the mounting seat 501 to rotate, namely, the injected culture solution can be evenly shaken and distributed, which is beneficial to the culture of cells, the top end of the rotating shaft 503 extends out of the rotating seat 502, the bottom end of the rotating shaft 503 extends out of the bottom end of the mounting frame 1, the use of the device is facilitated through the design, the culture mechanism 4 comprises a culture cavity 401, a limiting ring 402, sliding rods 403, springs 404, clamping strips 405, clamping grooves 406, a culture disc 407, mounting grooves 408 and fixing rings 409, a plurality of mounting grooves 408 are arranged on the mounting seat 501, the culture disc 407 is connected in the mounting grooves 408 in a sliding manner, the culture cavities 401 are arranged at the top ends of the culture discs 407, the fixing rings 409 are fixedly arranged at the outer sides of the bottom ends of the mounting seat 501, the limiting ring 402 is arranged at the upper part of the fixing rings 409, a plurality of sliding rods 403 are fixedly arranged at the top ends of the fixing rings 409, the top ends of the sliding rods 403 extend out of the limiting ring 402, the springs 404 are sleeved on the sliding rods 403, the equal fixed mounting in bottom of a plurality of springs 404 is on solid fixed ring 409, the equal fixed mounting in top of a plurality of springs 404 is on spacing ring 402, and the inboard of spacing ring 402 offsets with a plurality of cultivation dish 407 and sets up, through downwards pulling spacing ring 402, it is spacing to cultivate dish 407 this moment, the cultivation dish 407 that will take off is towards the outside pulling, make cultivation dish 407 break away from mounting groove 408, can take out the cell of cultivation, be convenient for the staff observes and studies, the equal fixed mounting in both sides of every cultivation dish 407 has card strip 405, card groove 406 has all been seted up to corresponding card strip 405 department in every mounting groove 408, card strip 405 is pegged graft in card groove 406, it is spacing to cultivateing dish 407 through card strip 405, be favorable to the use of this equipment.

The working principle is as follows: when the cell culture device is used, a switch of the water pump 301 is turned on, the water pump 301 conveys culture solution in the external storage box into the liquid injection box 302, the culture solution enters the liquid injection tray 305 through the connecting pipe 304, the electric push rod 303 drives the liquid injection tray 305 to descend, so that the nozzle 307 extends into the corresponding culture cavity 401, the electromagnetic valve 306 in the corresponding nozzle 307 is turned on as required, the culture solution can be added into the culture cavity 401 needing to be added with the culture solution, then the switch of the motor 506 is turned on, the motor 506 works to drive the second bevel gear 505 to rotate, the second bevel gear 505 drives the first bevel gear 504 to rotate, the first bevel gear 504 drives the rotating shaft 503 to rotate, the rotating shaft 503 drives the mounting seat 501 to rotate, the added culture solution can be uniformly shaken and distributed, when cultured cells need to be taken out for observation experiment, the limiting ring 402 is pulled downwards, the culture tray 407 loses the filling limit at the moment, the culture tray 407 is pulled towards the outer side, the culture tray 407 is separated from the mounting groove 408, the culture tray 407 can be taken out, the cultured cells can be taken out, the cell culture solution can be conveniently added according to different cell culture conditions, and the splashing culture solution can be added into the culture chamber 401, and the culture medium can be added, so that the culture solution can be added, and the waste of the culture solution can be further avoided;

through the mutual matching of the culture cavity 401, the limiting ring 402, the sliding rod 403, the spring 404, the clamping strip 405, the clamping groove 406, the culture disc 407, the mounting groove 408 and the fixing ring 409, the culture disc 407 and the mounting seat 501 can be quickly mounted and dismounted, so that a worker can conveniently take out cells to observe experiments, and meanwhile, the problems that cells need to be placed on a diaphragm for culture in the traditional culture process, the cells are difficult to take out due to the close fit between the diaphragm and the inner wall of a container, and the cells are easily damaged when the cells are taken out are solved;

through mutually supporting of mount pad 501, roating seat 502, pivot 503, first bevel gear 504, second bevel gear 505 and motor 506 for after filling the culture solution, can utilize mount pad 501 to rotate and make the culture solution rock evenly distributed in cultivateing chamber 401, be favorable to cell culture's use.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The cell culture device is characterized by comprising a mounting rack (1), an incubator (2), an injection mechanism (3), a culture mechanism (4) and a rotating mechanism (5), wherein the injection mechanism (3) comprises a water pump (301), an injection box (302), an electric push rod (303), a connecting pipe (304), an injection disc (305), an electromagnetic valve (306) and a nozzle (307), the incubator (2) is fixedly mounted on the mounting rack (1), the injection box (302) is fixedly mounted on the top end of the incubator (2), the water pump (301) is fixedly mounted on the right side of the injection box (302), the output end of the water pump (301) is communicated with the inside of the incubator (2), the connecting pipe (304) is fixedly mounted on the top end of the inside of the incubator (2), the injection disc (305) is fixedly mounted with the injection disc (304), the injection disc (305) is communicated with the inside of the injection box (302), the bottom end of the connecting pipe (304) is communicated with the inside of the injection disc (305), the injection disc (305) is fixedly mounted with a plurality of nozzles (307), the plurality of nozzles (307) are fixedly mounted on the outside of the injection disc (306), and the electric push rod (303) is mounted on the outside of the connecting pipe (304), the top ends of the electric push rods (303) are fixedly arranged at the top end in the incubator (2).

2. A cell culture device according to claim 1, wherein the connecting tube (304) is a gooseneck.

3. The cell culture device according to claim 1, wherein the rotating mechanism (5) comprises a mounting seat (501), a rotating seat (502), a rotating shaft (503), a first bevel gear (504), a second bevel gear (505) and a motor (506), the rotating seat (502) is fixedly mounted at the inner bottom end of the incubator (2), the rotating shaft (503) is rotatably connected to the rotating seat (502), the mounting seat (501) is fixedly mounted at the top end of the rotating shaft (503), the first bevel gear (504) is fixedly mounted at the bottom end of the rotating shaft (503), the motor (506) is fixedly mounted at the right side of the first bevel gear (504) at the bottom end of the mounting frame (1), the second bevel gear (505) is fixedly mounted at the output end of the motor (506), and the second bevel gear (505) is meshed with the first bevel gear (504).

4. A cell culture apparatus according to claim 3, wherein the top end of the rotary shaft (503) extends out of the rotary base (502), and the bottom end of the rotary shaft (503) extends out of the bottom end of the mounting frame (1).

5. The cell culture device according to claim 3, wherein the culture mechanism (4) comprises a culture cavity (401), a limiting ring (402), a plurality of sliding rods (403), springs (404), clamping strips (405), clamping grooves (406), a culture disc (407), a mounting groove (408) and a fixing ring (409), the mounting seat (501) is provided with a plurality of mounting grooves (408), the mounting groove (408) is internally and slidably connected with the culture disc (407), the top end of the culture disc (407) is provided with the culture cavity (401), the fixing ring (409) is fixedly mounted on the outer side of the bottom end of the mounting seat (501), the upper portion of the fixing ring (409) is provided with the limiting ring (402), the top end of the fixing ring (409) is fixedly provided with the plurality of sliding rods (403), the top ends of the plurality of sliding rods (403) extend out of the limiting ring (402), the plurality of sliding rods (403) are sleeved with the springs (404), the bottom ends of the plurality of the springs (404) are fixedly mounted on the fixing ring (409), the top ends of the plurality of the springs (404) are fixedly mounted on the fixing ring (409), and the limiting ring (402) is abutted against the inner side of the limiting ring (407).

6. The cell culture device according to claim 5, wherein clamping strips (405) are fixedly mounted on two sides of each culture disc (407), clamping grooves (406) are formed in the mounting grooves (408) corresponding to the clamping strips (405), and the clamping strips (405) are inserted into the clamping grooves (406).