CN201737949U - Cell culturing device capable of periodically converting positive pressure and negative pressure - Google Patents
Cell culturing device capable of periodically converting positive pressure and negative pressure Download PDFInfo
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- CN201737949U CN201737949U CN2010202553887U CN201020255388U CN201737949U CN 201737949 U CN201737949 U CN 201737949U CN 2010202553887 U CN2010202553887 U CN 2010202553887U CN 201020255388 U CN201020255388 U CN 201020255388U CN 201737949 U CN201737949 U CN 201737949U
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Abstract
The utility model discloses a cell culturing device capable of periodically converting positive pressure and negative pressure. A culturing room is provided with a pressure increasing device, a pressure decreasing device, an air pressure sensor, an air pressure control unit and a semi-permeable membrane, wherein a culturing liquid containing cavity is formed between the inner bottom surfaces of the semi-permeable membrane and the culturing room. When the cell culturing device is in use, the culturing liquid is injected into the culturing liquid containing cavity, the cell is transplanted on the semi-permeable membrane, the air pressure sensor detects the air pressure in the culturing room and transmits a signal to the air pressure control unit after staring up a pressure adjusting component, and the control unit can send out a pressure-decreasing instruction or a pressure-increasing instruction to a pressure-decreasing electric control valve or a pressure-increasing electric control valve after the air pressure of the culturing room reaches a preset positive pressure value or a preset negative pressure valve to periodically convert the positive pressure and the negative pressure by increasing and decreasing the pressure. The cell culturing device can be used for inspecting the change of the biological behavior of the cell such as the multiplication speed, the growth status and the like when the positive pressure and the negative pressure are converted and researching the relevant diseases, and is simple in structure, convenient to use, and stable and reliable in experiment.
Description
Technical field
The utility model relates to a kind of medical auxiliary apparatus, and particularly a kind of positive/negative pressure is the transition cell culture apparatus periodically.
Background technology
Cyto-mechanics is one of basis of organizational project and cell engineering, and one of its important contents is exactly to study the influence of mechanical stimulation pair cell system change and growth.The cell internal tension interaction of generation constituted a mechanical system when interior each tissue of body and intercellular interaction force (stretching, shearing) and cytoskeleton changed in the power outside the organism, the life movement, was regulating and was growing and cytodifferentiation.Power can brought into play important effect by influencing in the cell genetic expression and proteinic synthesizing regulating cell function in cells physiological, pathologic process.
Recent studies have shown that: the cell physiological conditions under malleation or negative pressure condition respectively is different.Endothelial cell be can damage as the intraocular pressure that increases rapidly, corneal edema, muddiness caused.Mechanically stressed change is that bone makes up and the basic motive of reinventing, and has confirmed that persistence pressure has restraining effect to scleroblast.And negative pressure system can work to many dissimilar cell proliferations, epidermic cell, inoblast and endotheliocyte etc.
In order to study the influence of pressure load cell growth, a lot of scholars have designed device with the suffered biological power of analogue body inner cell.The general power that applies to cell mainly contains four kinds: hydrodynamic shear, static pressure, twin shaft are to distraction force, non axial distraction force.Previously the device weak point is: 1, pressure load is single, can't realize positive negative pressure conversion, promptly can't observe the change with the biological behaviour of a kind of cell under different pressures; 2, the pressure in the incubator keeps having difficulty; 3, gnotobasis is wayward.
Be necessary to overcome described defective, periodically transition cell culture apparatus of a kind of positive/negative pressure is provided, the cell culture environment that can provide positive/negative pressure periodically to change, the biological behaviour of cell in the positive/negative pressure conversion changes and relative disease research provides safeguard in order to investigate.
The utility model content
In view of this, the purpose of this utility model provide a kind of simple in structure, make periodically transition cell culture apparatus of easy, easy to operate positive/negative pressure, the cell culture environment that can provide positive/negative pressure periodically to change, biological behaviours such as the multiplication rate of cell in the positive/negative pressure conversion, growth conditions change and relative disease research provides safeguard in order to investigate, and simple in structure, make easy, easy to operate.
Positive/negative pressure of the present utility model is the transition cell culture apparatus periodically, comprises culturing room, and culturing room is provided with pressure adjusting part and cell cultures assembly;
Described pressure adjusting part comprises pressurizing device, reliever, air pressure probe and air-pressure controlling unit, the pressurization mouth of pressurizing device, the pressure-reduction outlet of reliever are communicated with culturing room respectively, the pressure pipeline of pressurizing device is provided with the pressurization electrically-controlled valve, and the relief line of reliever is provided with the decompression electrically-controlled valve;
Described air-pressure controlling unit is provided with pressure signal input terminus, pressurization instruction output end and decompression instruction output end, the signal output part of air pressure probe links to each other with the pressure signal input terminus, the pressurization instruction output end links to each other with the instruction input terminus of pressurization electrically-controlled valve, and the decompression instruction output end links to each other with the command reception end of decompression electrically-controlled valve;
Described cell cultures assembly comprises with removably and is horizontally placed on semi-permeable membranes in the culturing room, form the nutrient solution cavity volume between semi-permeable membranes and the culturing room's inner bottom surface, the top that is positioned at the nutrient solution cavity volume in the culturing room is provided with semi-permeable membranes and imports and exports, semi-permeable membranes is imported and exported the sealing cover that is provided with the transparent observing interface, corresponding fluid inlet and the liquid outlet of being provided with the nutrient solution cavity volume on culturing room's sidewall.
Further, also comprise the nutrient solution recirculation assembly, described nutrient solution recirculation assembly comprises the nutrient solution tank, the nutrient solution tank is provided with circulation fluid inlet, circulation fluid outlet and fluid infusion mouth, described liquid outlet is communicated with the circulation fluid inlet, described fluid inlet is communicated with the circulation fluid outlet, on the nutrient solution circulation line recycle pump is set;
Further, recycle pump comprises recycle pump I and recycle pump II, and liquid outlet is communicated with by recycle pump I with the circulation fluid inlet, and fluid inlet is communicated with by recycle pump II with the circulation fluid outlet, on the circulation line of the circulation line of recycle pump I and recycle pump II electrically-controlled valve is set respectively;
Described fluid inlet and liquid outlet are provided with fluid pressure sensor respectively, also comprise the recycle pump control unit, described recycle pump control unit is provided with fluid pressure signal input terminus, instruction output end I and instruction output end II, the signal output part of fluid pressure sensor links to each other with the fluid pressure signal input terminus of recycle pump control unit, instruction output end I links to each other with the electrically-controlled valve of recycle pump I, and instruction output end II links to each other with the electrically-controlled valve of recycle pump II;
Further, described air pressure probe is at least 3 and be distributed in the culturing room, and described pressurizing device is a force (forcing) pump, and reliever is a drawdown pump;
Further, the fluid infusion mouth of nutrient solution tank is provided with filter membrane.
The beneficial effects of the utility model: positive/negative pressure of the present utility model is the transition cell culture apparatus periodically, comprises culturing room, and culturing room is provided with pressure adjusting part and cell cultures assembly; The pressure adjusting part comprises pressurizing device, reliever, air pressure probe and air-pressure controlling unit, the cell cultures assembly comprises the semi-permeable membranes that is horizontally placed in the culturing room, form nutrient solution cavity volume, corresponding fluid inlet and the liquid outlet of being provided with the nutrient solution cavity volume on the sidewall of chamber between semi-permeable membranes and the culturing room's inner bottom surface; During use, to the nutrient solution cavity volume, inject nutrient solution from fluid inlet and touch semi-permeable membranes until nutrient solution, again cell is planted in the semi-permeable membranes upper surface, behind the trigger pressure adjusting part, air pressure probe detects the air pressure in the culturing room at any time and signal is passed to the air-pressure controlling unit, when culturing room's air pressure reaches predetermined malleation value, control unit sends the decompression instruction to the decompression electrically-controlled valve, the reliever operation also reduces culturing room's internal pressure, after air pressure is reduced to predetermined negative pressure value, control unit sends the pressurization instruction again, so circulation, and then the positive/negative pressure in the realization culturing room is periodically changed; Biological behaviours such as the multiplication rate of cell in the positive/negative pressure conversion, growth conditions change and relative disease research provides safeguard in order to investigate, and culturing room's internal pressure environment is easy to keep stable simultaneously, and is simple in structure, easy to use, the experimental implementation good reliability.
Description of drawings
Below in conjunction with the drawings and specific embodiments the utility model is further described.
Fig. 1 is a structural representation of the present utility model;
Fig. 2 is an A portion enlarged view among Fig. 1;
Fig. 3 is the utility model schematic diagram.
Embodiment
Fig. 1 is a structural representation of the present utility model; Fig. 2 is an A portion enlarged view among Fig. 1; Fig. 3 is the utility model schematic diagram.
As shown in the figure:
The positive/negative pressure of present embodiment is the transition cell culture apparatus periodically, comprises culturing room 1, and culturing room 1 is provided with pressure adjusting part and cell cultures assembly; Described pressure adjusting part comprises pressurizing device 2, reliever 3, air pressure probe 4 and air-pressure controlling unit 5, the pressurization mouth of pressurizing device, the pressure-reduction outlet of reliever are communicated with culturing room respectively, the pressure pipeline of pressurizing device is provided with pressurization electrically-controlled valve 21, and the relief line of reliever is provided with decompression electrically-controlled valve 31;
Described air-pressure controlling unit is provided with the pressure signal input terminus, pressurization instruction output end and decompression instruction output end, the signal output part of air pressure probe 4 links to each other with the pressure signal input terminus, the pressurization instruction output end links to each other with the instruction input terminus of pressurization electrically-controlled valve 21, the decompression instruction output end links to each other with the command reception end of decompression electrically-controlled valve 31, during concrete enforcement, air-pressure controlling unit 5 comprises analog to digital converter 51 and control treater 52, the signal output part of analog to digital converter links to each other with the signal input part of control treater 52, described pressure signal input terminus is arranged on the analog to digital converter, and described pressurization instruction output end and decompression instruction output end are arranged on the control treater;
Described cell cultures assembly comprises with removably and is horizontally placed on semi-permeable membranes 6 in the culturing room, embodiment is: clamp semi-permeable membranes 6 with annular clamping device 61, culturing room's inwall is laid cushion cap 62 along a ring clamping device circumferentially is set, clamping device is provided with drag hook 63, certainly, it also can be handle, the culturing room top is provided with uncovered to import and export as semi-permeable membranes, the user holds handle or drag hook, clamping there is the clamping device 61 of semi-permeable membranes carry on a shoulder pole straight down on cushion cap 62 from the semi-permeable membranes import and export, and then with the sealing cover 15 that is provided with the transparent observing interface semi-permeable membranes is imported and exported to close and get final product, being convenient to semi-permeable membranes inserts, take out and change, form nutrient solution cavity volume 7, corresponding fluid inlet 71 and the liquid outlet 72 of being provided with the nutrient solution cavity volume on culturing room's sidewall between semi-permeable membranes 6 and the culturing room's inner bottom surface; During use, to nutrient solution cavity volume 7, inject nutrient solution from fluid inlet 71 and touch semi-permeable membranes 6 until nutrient solution, again cell is planted in semi-permeable membranes 6 upper surfaces, behind the trigger pressure adjusting part, air pressure probe 4 detects the air pressure in the culturing room 1 at any time and signal is passed to air-pressure controlling unit 5, when culturing room's air pressure reaches predetermined malleation value, control unit sends the decompression instruction to the decompression electrically-controlled valve, the reliever operation also reduces culturing room's internal pressure, after air pressure is reduced to predetermined negative pressure value, control unit sends the pressurization instruction again, so circulation, and then the positive/negative pressure in the realization culturing room is periodically changed; Whole device can be used for investigating biological behaviours such as the multiplication rate of cell in the positive/negative pressure conversion, growth conditions and changes and relative disease research, and is simple in structure, easy to use, is easy to keep stable because of culturing room's internal pressure environment simultaneously, and experimental reliability is higher.In the present embodiment, culturing room and sealing cover are made by transparent material, are beneficial to the upgrowth situation of observation of cell, and the user also can be provided with thief hole as required on sealing cover or culturing room's locular wall, and thief hole is convenient to interim a small amount of application of sample or sampling by sealing of lid.
Further improvement as previous embodiment, also comprise the nutrient solution recirculation assembly, described nutrient solution recirculation assembly comprises nutrient solution tank 8, the nutrient solution tank is provided with circulation fluid inlet 81, circulation fluid outlet 82 and fluid infusion mouth 83, described liquid outlet 72 is communicated with circulation fluid inlet 81, described fluid inlet 71 is communicated with circulation fluid outlet 82, on the nutrient solution circulation line recycle pump is set; The nutrient solution tank can be used for the buffer memory of nutrient solution, by the nutrient solution circulation, improves utilization ratio of raw materials, saves cost, and the user also can inject fresh medium by the fluid infusion mouth in the nutrient solution tank, be convenient to carry out as required the replacement of nutrient solution.
Further improvement as previous embodiment, recycle pump comprises recycle pump I 9 and recycle pump II10, liquid outlet 72 and circulation fluid inlet 81 is communicated with by recycle pump I 9, fluid inlet 71 and circulation fluid outlet 82 is communicated with by recycle pump II 10, electrically-controlled valve 91 is set on the circulation line of recycle pump I 9, electrically-controlled valve 101 is set on the circulation line of recycle pump II 10; Fluid inlet 71 is provided with fluid pressure sensor 11, liquid outlet 72 is provided with fluid pressure sensor 12, also comprise recycle pump control unit 13, described recycle pump control unit is provided with fluid pressure signal input terminus, instruction output end I and instruction output end II, the signal output part of fluid pressure sensor 11 and fluid pressure sensor 12 links to each other with the fluid pressure signal input terminus of recycle pump control unit respectively, instruction output end I links to each other with the electrically-controlled valve 91 of recycle pump I 9, and instruction output end II links to each other with the electrically-controlled valve 101 of recycle pump II; Recycle pump I 9 and recycle pump II 10 can strengthen the circulation of nutrient solution, at fluid inlet and liquid outlet fluid pressure sensor is set respectively, can detect the hydrodynamicpressure at fluid inlet position and liquid outlet position, when two place's hydrodynamicpressures not simultaneously, the recycle pump control unit sends instruction to electrically-controlled valve 91 or electrically-controlled valve 101, by liquid feeding in the nutrient solution cavity volume, discharge opeing or adjusting nutrient solution flow velocity, make the hydrodynamicpressure at fluid inlet and leakage fluid dram position keep balance, guarantee the stable of cell culture environment, during concrete enforcement, recycle pump control unit 13 comprises analog to digital converter 131 and control treater 132, the analog to digital converter signal output part links to each other with control processor signal input terminus, the fluid pressure signal input terminus is arranged on the analog to digital converter 131, and instruction output end I and instruction output end II are arranged on the control treater 132; In the present embodiment, air-pressure controlling unit 5 and recycle pump control unit 13 are shared, as shown in Figure 2, are convenient to reduce cost, and improve utilization ratio of device.
As the further improvement of previous embodiment, described air pressure probe is at least 3 and be distributed in the culturing room; Embodiment is 3, and a plurality of air pressure probes can detect the air pressure of different positions respectively, and reliability is higher, and described pressurizing device is a force (forcing) pump, and reliever is drawdown pump, and is with low cost, is easy to obtain.
As the further improvement of previous embodiment, the fluid infusion mouth of nutrient solution tank is provided with filter membrane 14, but elimination impurity during fluid infusion reduces and disturbs.
Explanation is at last, above embodiment is only unrestricted in order to the explanation the technical solution of the utility model, although the utility model is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the technical solution of the utility model, and not breaking away from the aim and the scope of technical solutions of the utility model, it all should be encompassed in the middle of the claim scope of the present utility model.
Claims (5)
1. positive/negative pressure transition cell culture apparatus periodically, it is characterized in that: comprise culturing room (1), culturing room (1) is provided with pressure adjusting part and cell cultures assembly;
Described pressure adjusting part comprises pressurizing device (2), reliever (3), air pressure probe (4) and air-pressure controlling unit (5), the pressurization mouth of pressurizing device, the pressure-reduction outlet of reliever are communicated with culturing room (1) respectively, the pressure pipeline of pressurizing device (2) is provided with pressurization electrically-controlled valve (21), and the relief line of reliever (3) is provided with decompression electrically-controlled valve (31);
Described air-pressure controlling unit (5) is provided with pressure signal input terminus, pressurization instruction output end and decompression instruction output end, the signal output part of air pressure probe (4) links to each other with described pressure signal input terminus, described pressurization instruction output end links to each other with the instruction input terminus of pressurization electrically-controlled valve (21), and described decompression instruction output end links to each other with the command reception end of decompression electrically-controlled valve (31);
Described cell cultures assembly comprises with removably and is horizontally placed on semi-permeable membranes (6) in the culturing room, form nutrient solution cavity volume (7) between semi-permeable membranes (6) and the culturing room's inner bottom surface, the top that is positioned at the nutrient solution cavity volume in the culturing room is provided with semi-permeable membranes and imports and exports, semi-permeable membranes is imported and exported the sealing cover that is provided with the transparent observing interface, corresponding fluid inlet (71) and the liquid outlet (72) of being provided with the nutrient solution cavity volume on culturing room's sidewall.
2. positive/negative pressure according to claim 1 is the transition cell culture apparatus periodically, it is characterized in that: also comprise the nutrient solution recirculation assembly, described nutrient solution recirculation assembly comprises nutrient solution tank (8), the nutrient solution tank is provided with circulation fluid inlet (81), circulation fluid outlet (82) and fluid infusion mouth (83), described liquid outlet (72) is communicated with circulation fluid inlet (81), described fluid inlet (71) is communicated with circulation fluid outlet (82), on the nutrient solution circulation line recycle pump is set.
3. positive/negative pressure according to claim 2 is the transition cell culture apparatus periodically, it is characterized in that: recycle pump comprises recycle pump I (9) and recycle pump II (10), liquid outlet (72) is communicated with by recycle pump I (9) with circulation fluid inlet (81), fluid inlet (71) is communicated with by recycle pump II (10) with circulation fluid outlet (82), on the circulation line of the circulation line of recycle pump I (9) and recycle pump II (10) electrically-controlled valve is set respectively;
Described fluid inlet (71) and liquid outlet (72) are provided with fluid pressure sensor respectively, also comprise recycle pump control unit (13), described recycle pump control unit is provided with fluid pressure signal input terminus, instruction output end I and instruction output end II, the signal output part of fluid pressure sensor links to each other with the fluid pressure signal input terminus of recycle pump control unit, instruction output end I links to each other with the electrically-controlled valve of recycle pump I (9), and instruction output end II links to each other with the electrically-controlled valve of recycle pump II (10).
4. positive/negative pressure according to claim 3 is the transition cell culture apparatus periodically, it is characterized in that: described air pressure probe is at least 3 and be distributed in the culturing room, and described pressurizing device is a force (forcing) pump, and reliever is a drawdown pump.
5. positive/negative pressure according to claim 4 is the transition cell culture apparatus periodically, and it is characterized in that: the fluid infusion mouth of nutrient solution tank is provided with filter membrane (14).
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010202553887U CN201737949U (en) | 2010-07-12 | 2010-07-12 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
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| CN2010202553887U CN201737949U (en) | 2010-07-12 | 2010-07-12 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
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| CN201737949U true CN201737949U (en) | 2011-02-09 |
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| CN2010202553887U Expired - Fee Related CN201737949U (en) | 2010-07-12 | 2010-07-12 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
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| CN113444642A (en) * | 2021-07-29 | 2021-09-28 | 中国科学院长春光学精密机械与物理研究所 | Pneumatic system for cell loading and control method |
| CN116333882A (en) * | 2023-03-22 | 2023-06-27 | 浙江大学 | Soft cell culture dish capable of realizing radial pulling and pressing and out-of-plane bending |
| CN116333882B (en) * | 2023-03-22 | 2023-10-31 | 浙江大学 | Soft cell culture dish capable of realizing radial pulling and pressing and out-of-plane bending |
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