CN206502827U - A kind of NSC continuously changes liquid culture apparatus - Google Patents

Abstract

The utility model is related to a kind of NSC and continuously changes liquid culture apparatus, provided with Tissue Culture Flask, left side of the Tissue Culture Flask in bottom, right side is respectively arranged with left side drain pipe, right side liquid back pipe, the left side drain pipe, right side liquid back pipe passes through a woven hose, peristaltic pump is connected with the screen cloth pipe being arranged in grass tube, the woven hose is provided with left end enclosed switch, right-hand member enclosed switch, the screen cloth pipe is provided with the micropore that aperture is 50 microns, the Tissue Culture Flask adds mouth provided with new bottle nutrient solution is trained, the grass tube is a thicker container-like pipeline, the container-like pipeline passes through collecting peristaltic pump respectively at two ends, circulation pipe is collected with collector to be connected, the collector is provided with the new culture medium addition mouth of collector, screen cloth film is provided with the collector, the aperture of the screen cloth film is 10 microns.

Description

A kind of NSC continuously changes liquid culture apparatus

Technical field

The utility model belongs to a kind of cell culture apparatus, and particularly a kind of NSC is continuously cultivated and changes liquid culture Device.

Background technology

NSC：NSC (neural stem cell) is a kind of lifelong thin with self-renewal capacity Born of the same parents, its daughter cell can break up the various types of cells for producing nervous system, with limited self-renewal capacity, and Spontaneous Differentiation is produced Into neuronal cell and spongioblast etc., so as to generate neuron and Deiter's cells.After transplanting can host nerve Existence well in tissue, it is whole and and differentiation, the treatment of reparation and Other diseases for craniocerebral injury has a wide range of applications Prospect.

Current NSC in vitro culture mode mainly concentrates the culture that suspends in culture, and NSC can be in culture During can produce a large amount of cell residues and metabolic waste from unicellular slowly glomeration, and in incubation, to thin Born of the same parents' progress changes liquid and can remove the cell residue in nutrient solution and metabolic waste, so that cell proliferation plays a part of promotion.

The major way that liquid is changed NSC in laboratory is to stand blake bottle, with common pipette, extract culture medium Supernatant liquid carries out changing liquid removal.Current technological deficiency:The mode of current Culture of neural stem cells mainly concentrates outstanding in culture Floating culture, is suctioned out the old culture medium of 2/3 volume in blake bottle with pipette when changing liquid.Because NSC was being cultivated Integrating cell group is assembled in journey, bottom of bottle can be deposited to by larger, heavier mass cell mass by a part of volume by standing, but still have big Part cell mass does Brownian movement in culture concentration, can suspend in the medium, and draws upper strata currently with conventional pipette 2/3 culture medium is discarded, and the nozzle diameter of pipette is general in 1mm or so, much larger than cell mass diameter, therefore during liquid is changed Substantial amounts of loss cell is had, and the culture medium removed during liquid is changed is excessive, and nutritional ingredient is not yet consumed in culture medium, Culture medium is caused to waste.

Utility model content

The purpose of this utility model is that, in order to solve the above problems, a kind of NSC of proposition continuously changes liquid culture dress Put, the NSC continuously changes liquid culture apparatus and solved during Culture of neural stem cells changes liquid, NSC suspends Being replaced together during liquid is changed with cell residue in old culture medium causes to waste, while making media flow, makes nerve Ball suspension growth, prevents cell attachment from breaking up.

The purpose of this utility model is achieved through the following technical solutions：NSC continuously changes liquid culture dress Put, it is characterised in that：Provided with Tissue Culture Flask, the Tissue Culture Flask is respectively arranged with left side on left side, the right side of bottom and gone out Liquid pipe, right side liquid back pipe, the left side drain pipe, right side liquid back pipe is by a woven hose, peristaltic pump and is arranged on grass tube Interior screen cloth pipe is connected, and the woven hose is provided with left end enclosed switch, right-hand member enclosed switch, and the screen cloth pipe is provided with hole Footpath is 50 microns of micropore, and the Tissue Culture Flask is one thicker provided with new bottle nutrient solution addition mouth, the grass tube is trained Container-like pipeline, the container-like pipeline passes through collecting peristaltic pump at two ends, collects circulation pipe and collector phase respectively Connection, the collector is provided with the new culture medium addition mouth of collector, and the collector is interior to be provided with screen cloth film, the screen cloth film Aperture is 10 microns.

The characteristics of new utilization NSC of this reality is gathered into cell mass in incubation, according to cell mass and cell Residue volume difference in size be separated by filtration changing liquid；Filter carries out residue separation using bernoulli principle using misorientation stream Improve separative efficiency；It is collected using slipstream, prevents residue from blocking screen cloth and culture medium can be made to be followed again after following filtering Ring；Organic polymer plastic and glass is used for material, it is to avoid cytoadherence and convenient sterilizing；Closing seam, it is ensured that cell Germ-free condition during culture；Can carry out long lasting for residue separation change liquid, add new culture medium mixing, the residue that swaps out is mixed Liquid is closed, continuous pure medium improves NSC concentration in blake bottle；Culture medium is recycled in a device, is carried High culture medium service efficiency.

Brief description of the drawings

Fig. 1 is structural representation of the present utility model；

Embodiment

The utility model is further described with reference to the accompanying drawings and examples：

Embodiment：Referring to accompanying drawing 1, NSC continuously changes liquid culture apparatus, provided with Tissue Culture Flask 1, the cell Blake bottle is respectively arranged with left side drain pipe, right side liquid back pipe on left side, the right side of bottom, and the left side drain pipe, right side are returned Liquid pipe is connected by a woven hose, peristaltic pump 2 with the screen cloth pipe 3 being arranged in grass tube 4, and the woven hose is provided with a left side Enclosed switch 7, right-hand member enclosed switch 8 are held, the screen cloth pipe is provided with the micropore that aperture is 50 microns, the Tissue Culture Flask Provided with new bottle nutrient solution addition mouth 9 is trained, the grass tube is a thicker container-like pipeline, and the container-like pipeline is at two ends It is connected respectively by collecting peristaltic pump 10, collection circulation pipe with collector, the collector is newly trained provided with collector Support and screen cloth film 5 is provided with base addition mouth 6, the collector, the aperture of the screen cloth film is 10 microns.

Culture of neural stem cells early stage, NSC is in unicellular distribution, and cell is now seeded in the training shown in 1 Support in bottle, the switches of shown in figure 7 and 8 positions closed, makes cell quiescent culture in blake bottle, after three days cell aggregation into After ball, by new nutrient solution, two addition mouth injections from figure, open the switch of 7 and 8 positions, open two peristaltic pumps, make liquid Circulate and carry out according to the direction shown in figure, and two liquid in pipe flow velocity sizes are variant, it is ensured that the liquid of connection blake bottle Body flow rate of pipelines is less than the flow rate of liquid of the pipeline of connection filter, and the big pressure of flow velocity is small, when liquid travels from liquid intersection, The different misorientation stream of two pipeline formation flow velocitys, makes the less cell residue of diameter be gone out through membrane filtration, and larger god Then stay in blake bottle pipeline through ball to continue cycling through, misorientation, which flows through filter, can improve filter efficiency and rinse screen cloth holding cleaning, Cell residue after filtering enters collector with pipeline, and slipstream is formed at 5 filter membranes formed makes cell residue filtering removal, And ensure residue non clogging screen net, whole experimental provision dynamic circulation, it is ensured that neural ball, which suspends, to be cultivated, and it is residual to remove cell Slag, and culture medium is recycled, improve culture medium utilization ratio.

Finally it should be noted that：Obviously, above-described embodiment is only intended to clearly illustrate the utility model example, And the not restriction to embodiment.For those of ordinary skill in the field, may be used also on the basis of the above description To make other changes in different forms.There is no necessity and possibility to exhaust all the enbodiments.And thus Among the obvious changes or variations extended out is still in protection domain of the present utility model.

Claims (1)

1. a kind of NSC continuously changes liquid culture apparatus, it is characterised in that：Provided with Tissue Culture Flask (1), the cell training Support bottle and left side drain pipe, right side liquid back pipe are respectively arranged with left side, the right side of bottom, liquid is returned on the left side drain pipe, right side Pipe is connected by a woven hose, peristaltic pump (2) with the screen cloth pipe (3) being arranged in grass tube (4), is set on the woven hose There are left end enclosed switch (7), right-hand member enclosed switch (8), the screen cloth pipe is provided with the micropore that aperture is 50 microns, the cell Blake bottle is provided with new bottle nutrient solution addition mouth (9) is trained, and the grass tube is a thicker container-like pipeline, described container-like Pipeline is connected by collecting peristaltic pump (10), collection circulation pipe with collector respectively at two ends, is set on the collector Have and screen cloth film (5) is provided with the new culture medium addition mouth (6) of collector, the collector, the aperture of the screen cloth film is 10 microns.